id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-296362-9vi8xwu7	Wang, Jian-Min	Construction of a non-infectious SARS coronavirus replicon for application in drug screening and analysis of viral protein function	2008-09-12		.txt	text/plain	3490	199	57	title: Construction of a non-infectious SARS coronavirus replicon for application in drug screening and analysis of viral protein function Based on the infectious cDNA clone of rSARS-CoV-DE, in which the E gene has been deleted, a safe non-infectious replicon was constructed by replacing the S gene with the enhanced green fluorescent protein (eGFP) gene. Based on the infectious cDNA clone of rSARS-CoV-DE, in which the E gene has been deleted, a safe non-infectious replicon was constructed by replacing the S gene with the enhanced green fluorescent protein (eGFP) gene. For in-depth study of functions of different viral proteins and regulatory sequence elements by reverse genetics, full-length infectious cDNA clones have been established using various techniques including bacterial artificial chromosome (BAC) vector [23] [24] [25] and SARS-CoV replicon cell lines [26] , which can also be used for antiviral drug screening. Construction of a severe acute respiratory syndrome coronavirus infectious cDNA clone and a replicon to study coronavirus RNA synthesis	./cache/cord-296362-9vi8xwu7.txt	./txt/cord-296362-9vi8xwu7.txt