id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-259603-bh198xgl	Snijder, E.J.	The Nonstructural Proteins Directing Coronavirus RNA Synthesis and Processing	2016-09-14		.txt	text/plain	24187	1090	50	Reverse-genetics studies targeting specific residues in SARS-CoV nsp7 confirmed the protein's importance for virus replication (Subissi et al., 2014b) , although the impact of single point mutations was smaller than anticipated on the basis of the biochemical characterization of the RNA-binding properties of nsp7-containing protein complexes in vitro (see later). The large number of viral subunits in these complexes (Subissi et al., 2014a) , the likely requirement for host factors (van Hemert et al., 2008) , and the concept of RNA synthesis occurring in a dedicated microenvironment in the infected cell (Knoops et al., 2008; V'Kovski et al., 2015) complicate the straightforward characterization of the CoV RdRp. To reconstitute the enzyme's activities in vitro, purified recombinant nsp12 is a key reagent but, for many years, such studies were hampered by poor nsp12 expression in Escherichia coli.	./cache/cord-259603-bh198xgl.txt	./txt/cord-259603-bh198xgl.txt