id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-254395-tu4aqczj	Froggatt, Heather M.	Development of a Fluorescence-Based, High-Throughput SARS-CoV-2 3CL(pro) Reporter Assay	2020-10-27		.txt	text/plain	4200	254	50	This experimentally optimized reporter assay allows for antiviral drug screening in human cell culture at biosafety level 2 (BSL2) with high-throughput compatible protocols. This reporter-based assay allows for antiviral drug screening in human cell culture at biosafety level 2 (BSL2) with high-throughput compatible sample processing and analysis. With the aim of generating a protease reporter compatible with SARS-CoV-2 and other present and future coronaviruses to support viral inhibitor screening, we selected CoV 3CL pro as our protease target. (C) Quantification of fluorescence from 293T cells 48 h after transfection with each FlipGFP reporter and either the SARS-CoV-2 3CL pro or an influenza virus protein (A/PR8/1834 NP). To observe whether these FlipGFP constructs background fluoresced without CoV 3CL pro activity, we transfected cells with each reporter or a superfolder GFP (sfGFP) expression plasmid. Development of a FlipGFP CoV 3CL pro reporter-based assay for protease inhibitor screening in human cells.	./cache/cord-254395-tu4aqczj.txt	./txt/cord-254395-tu4aqczj.txt