id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-333675-vkk2frnf	Hamada, Manabu	Synthesis and broad spectrum antiviral evaluation of bis(POM) prodrugs of novel acyclic nucleosides	2013-07-04		.txt	text/plain	4256	234	56	A series of seventeen hitherto unknown ANP analogs bearing the (E)-but-2-enyl aliphatic side chain and modified heterocyclic base such as cytosine and 5-fluorocytosine, 2-pyrazinecarboxamide, 1,2,4-triazole-3-carboxamide or 4-substituted-1,2,3-triazoles were prepared in a straight approach through an olefin acyclic cross metathesis as key synthetic step. N 1crotyl-1,2,4-triazole-3-bis-Boc-carboxamide 19 was then subjected to the olefin cross metathesis reaction, with bis(POM)-allylphosphonate in CH 2 Cl 2 to obtain the desired compound 20 in 16% yield. The title bis(POM) (E)-4-phosphono-but-2-en-1-yl acyclic nucleosides were subjected to an in vitro antiviral screening using a wide spectrum of viruses, in MDKC cell cultures for anti-influenza virus activity, in Vero cell cultures for an antiviral activity against Para-influenza3 shown) only compound 31 showed activity at an EC 50 of w10 mM (AD-169 strain) with no observed cytotoxicity at 100 mM ( Table 2) .	./cache/cord-333675-vkk2frnf.txt	./txt/cord-333675-vkk2frnf.txt