id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-255862-84u3c33m	Kim, Ji Won	Antiviral escin derivatives from the seeds of Aesculus turbinata Blume (Japanese horse chestnut)	2017-07-01		.txt	text/plain	2843	172	61	In this research, we investigated whether escin derivatives 1–7 (including new compounds 2, 3, 5 and 6), without the angeloyl or tigloyl groups and with modified glycosidic linkages by hydrolysis, have PEDV inhibitory effects with less cytotoxicity. Interestingly, compounds 1-7 isolated from the fraction with the two-step hydrolysis were evaluated to have much lower cytotoxic effects than compounds 8-10 from the n-BuOH part at concentration of 20 lM (Fig. S22) . As compounds 8-10 showed strong cytotoxic effects on Vero cells at 20 lM, their PEDV inhibitory activities were evaluated at a concentration of 2 lM. 33 To measure the expression level of viral RNA encoding nucleocapsid and spike proteins, compounds 4 and 6 were treated in Vero cells at a concentration of 40 lM and total RNA was extracted for reverse transcription followed by polymerase chain reaction using the primers for PEDV (STable 1 ).	./cache/cord-255862-84u3c33m.txt	./txt/cord-255862-84u3c33m.txt