id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001017-4qfhltg4	Chatterjee, Dhriti	Microglia Play a Major Role in Direct Viral-Induced Demyelination	2013-06-20		.txt	text/plain	6654	312	42	Previous studies demonstrated that microglia can be persistently infected by neurotropic mouse hepatitis virus (MHV) which causes meningoencephalitis, myelitis with subsequent axonal loss, and demyelination and serve as a virus-induced model of human neurological disease multiple sclerosis (MS). In our current studies, we have used RSA59 infection in vivo, in vitro, and ex vivo as a model to understand whether MHV can directly infect CNS resident microglia and the mechanism of microglial activation in the induction of chronic demyelination. To confirm the RSA59-induced CNS inflammation, brain and spinal cord sections from day 7 (peak of inflammation) and day 30 (peak of demyelination) postinfected mice were stained with H&E or LFB and examined. While Iba1 immunofluorescence was observed in both gray and white matter, double fluorescence/immunofluorescence demonstrated dual labelling of EGFP (viral antigen) positive Iba1 positive microglia/macrophages were present only in the white matter of RSA59 infected mice (Figure 1 ).	./cache/cord-001017-4qfhltg4.txt	./txt/cord-001017-4qfhltg4.txt