id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-348547-wmvqvbqi	Desmons, Aurore	Post-mortem diagnosis, of cytomegalovirus and varicella zoster virus co-infection by combined histology and tissue molecular biology, in a sudden unexplained infant death	2013-10-31		.txt	text/plain	2317	130	45	title: Post-mortem diagnosis, of cytomegalovirus and varicella zoster virus co-infection by combined histology and tissue molecular biology, in a sudden unexplained infant death Objectives To describe post-mortem combined histological and tissue molecular biological techniques for the diagnosis of cytomegalovirus and varicella zoster virus co-infection as a cause of death. Study design Real-time quantitative PCR and RT-PCR assays for Herpesviruses, respiratory viruses, Adenovirus, Enterovirus and Parvovirus B19 were performed on multi-organ frozen samples and paraffin-embedded tissues in combination with histology. Conclusions This case shows that thorough quantitative virological investigations on frozen tissues must be performed in combination with histology and immunohistochemistry for the determination of the cause of a sudden unexplained infant death. Post-mortem virological quantitative molecular analyses performed on frozen tissue samples revealed cytomegalovirus (CMV) and varicella zoster virus (VZV), mainly in the lungs. However, pulmonary necrotizing inflammatory lesions, immunohistochemistry, and molecular detection of CMV and VZV in multi-organs samples demonstrated the role of the co-infection in the death.	./cache/cord-348547-wmvqvbqi.txt	./txt/cord-348547-wmvqvbqi.txt