id author title date pages extension mime words sentences flesch summary cache txt cord-012354-2f5oq9e5 Holtkötter, Hannah Forensic differentiation between peripheral and menstrual blood in cases of alleged sexual assault—validating an immunochromatographic multiplex assay for simultaneous detection of human hemoglobin and D-dimer 2017-10-23 .txt text/plain 4604 220 53 Moreover, we aimed to evaluate (1) the sensitivity of the PMB test by considering serial dilutions of peripheral blood and menstrual fluid, (2) possible influences of and cross-reactivity with other biological secretions, and blood from various species, (3) possible false-positive results on blood from aged donors and deceased's peripheral blood, (4) the viability of DNA extraction and amplification from the residual blood diluted on the remaining buffer, (5) a case example involving a historical ethnological artifact, and (6) the performance of the test on blood samples from ten cases of alleged sexual assault. Best results, with clear, balanced signals for both hemoglobin and D-dimer presence, were received with a concentration of 360 nl of menstrual fluid (3 μl in 1000 μl buffer), Fig. 2 Heat maps of a signals for D-dimer presence with the 300 and 400 ng/ml cut-off (donor 1 to 16 = dried blood, donor 17 = liquid blood), b the sensitivity study, c the mixtures and cross-reactivity testing, d blood collected from aged donors, and e postmortem blood samples. ./cache/cord-012354-2f5oq9e5.txt ./txt/cord-012354-2f5oq9e5.txt