id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-295727-s63lffi8	Lima, Luciana	Trypanosoma livingstonei: a new species from African bats supports the bat seeding hypothesis for the Trypanosoma cruzi clade	2013-08-03		.txt	text/plain	7191	371	46	Barcoding, phylogenetic analyses and genetic distances based on SSU rRNA and gGAPDH supported these trypanosomes as a new species, which we named Trypanosoma livingstonei n. In this study, we isolated and characterised 14 new trypanosomes from African bats captured in Mozambique, southeast Africa, by inferring phylogenetic relationships using ribosomal SSU rRNA, gGAPDH and SL genes. In this study, barcoding using V7V8 SSU rRNA revealed that all new isolates from African bats shared high sequence similarity; 2-3 cloned sequences were determined for each isolate, and they tightly clustered together and were virtually identical (~0.2% of divergence) and different from any previously reported trypanosome species. Here, the new bat isolates were initially positioned using independent gGAPDH ( Figure 3 ) and SSU rRNA (data not shown) sequences in phylogenetic trees comprising representative species of all major trypanosome clades. In all phylogenetic trees, the new bat isolates formed a well-supported clade close to Australian trypanosomes (10% divergence) and basal to the T.	./cache/cord-295727-s63lffi8.txt	./txt/cord-295727-s63lffi8.txt