id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-287748-co9j3uig	Kobayashi, Tomoya	Detection of bat hepatitis E virus RNA in microbats in Japan	2018-05-29		.txt	text/plain	1388	70	55	Several recent studies have reported that various bat species harbor bat hepatitis E viruses (BatHEV) belonging to the family Hepeviridae, which also contains human hepatitis E virus (HEV). Here, we collected and screened 81 bat fecal samples from nine bat species in Japan to detect BatHEV RNA by RT-PCR using HEV-specific primers, and detected three positive samples. These data support the first detection of BatHEVs in Japanese microbats, indicating their wide geographical distribution among multiple bat species. BLAST analysis indicated that BtHEV-Ej1/-Ej2 showed the highest sequence identities to BatHEV/BS7, a German strain detected from the Serotine bat (Eptesicus serotinus), among strains previously reported in other countries. The closely related BatHEVs (BtHEV-Ej1/-Ej2 and Bat HEV/BS7) have been detected in different species of Eptesicus bats (E. PCR amplifications were performed using the KOD FX Neo (Toyobo) with consensus HEV primer sets (PanHEV F and R), which were designed in this study to amplify a 191-bp fragment of the RNA-dependent into Orthohepevirus D, in Japanese bats, suggesting wide geographical distribution of BatHEV among multiple bat species.	./cache/cord-287748-co9j3uig.txt	./txt/cord-287748-co9j3uig.txt