id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-354325-r73datur	Berger, Mitchell	Therapeutic Applications of Monoclonal Antibodies	2002-07-31		.txt	text/plain	12331	666	41	Attempts to use mouse myeloma cells to create hybrids and derive human MAbs led to the loss of human chromosomes and the inability to make human Igs. 13 Unfortunately, in vitro immunization is limited by its inability to produce a secondary response and by the absence of the affinity maturation process that occurs in vivo. In these transgenic mouse models, human antibodies with high affinity to an immunized antigen are naturally selected by the murine immune system via an affinity maturation process, and thereby show increased diversity of the MAbs. Transgenic mice may be a suitable alternative to chimeric or humanized antibody production or the use of phage display systems to create less immunogenic or novel antibodies. [43] [44] [45] Humanizing Monoclonal Antibodies Rodent MAbs with excellent affinities and specificities have been generated using conventional hybridoma technology, but their use in clinical medicine is limited due to the immune responses they elicit in humans.	./cache/cord-354325-r73datur.txt	./txt/cord-354325-r73datur.txt