id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-342242-cynpob7b	Godakova, Svetlana A.	Camelid VHHs Fused to Human Fc Fragments Provide Long Term Protection Against Botulinum Neurotoxin A in Mice	2019-08-07		.txt	text/plain	7485	386	54	Based on the analysis of B11-Fc and G3-Fc clones' circulation time in the serum (presence of antibodies 14 days after injection), we decided to conduct an experiment on the survival of these mice, which previously received a single injection of the VHHs with the Fc fragment, with a repeated administration of only the lethal toxin dose 14 days after the original administration. Overall, we obtained numerous clones after two rounds of biopanning; we selected 15 clones for initial analysis based on their CDR3s, chose two clones (B11 and G3) with the best pre-mixed results in phage form in vivo, produced them in protein form, and modified their structure and characteristics by dimerization via a (Gly4Ser) 3 linker and fusion to a human IgG Fc fragment to enhance their protective activity.	./cache/cord-342242-cynpob7b.txt	./txt/cord-342242-cynpob7b.txt