id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-337464-otwps68u	Parray, Hilal Ahmed	Hybridoma technology a versatile method for isolation of monoclonal antibodies, its applicability across species, limitations, advancement and future perspectives	2020-05-27		.txt	text/plain	12204	606	38	Even with the recent development of high throughput mAb generation technologies, hybridoma is the most favoured method due to its indigenous nature to preserve natural cognate antibody pairing information and preserves innate functions of immune cells. This review also summarizes the challenges and recent progress associated with hybridoma development, and how it has been overcome in these years to provide new insights for the isolation of mAbs. Antibodies are the glycoproteins produced by the B-cells also known as immunoglobulins, which are present in higher eukaryotes. The mice hybridoma technology is a multi-step process that takes advantage of a host animal's natural ability to produce highly specific, high-affinity and fully functional mAbs. It involves the development and optimization of specific immunogenic antigen (Ag).	./cache/cord-337464-otwps68u.txt	./txt/cord-337464-otwps68u.txt