id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-336280-tsx409e3	Byrne, Barry	Antibody-Based Sensors: Principles, Problems and Potential for Detection of Pathogens and Associated Toxins	2009-06-05		.txt	text/plain	12263	613	38	Particular emphasis is placed on the detection of foodborne bacterial pathogens, such as Escherichia coli and Listeria monocytogenes, and additional examples relating to the monitoring of fungal pathogens, viruses, mycotoxins, marine toxins and parasites are also provided. It describes the development of electrochemical, potentiometric, piezoelectric and optical platforms for the monitoring of foodborne bacterial pathogens by incorporating monoclonal, polyclonal or recombinant antibodies in a variety of different assay formats. Polyclonal antibodies (pAb) are typically raised in rabbits, goats or sheep [29] , and their popularity is illustrated by the fact that they are frequently selected in immunosensor-based assays for pathogen detection. Hence, the selection of a highly-specific epitope on the pathogen is a key consideration, since many bacterial strains share homologues of surface-presented proteins which can lead to the detection of multiple cell-types by a single antibody. Muhammad-Tahir and Alocilja [74] developed a conductimetric biosensor incorporating a polyclonal antibody-based sandwich assay format in which the detection antibody was labelled with polyaniline.	./cache/cord-336280-tsx409e3.txt	./txt/cord-336280-tsx409e3.txt