id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-335311-l73hsik0	Chan, Conrad E. Z.	The role of phage display in therapeutic antibody discovery	2014-08-18		.txt	text/plain	7044	286	31	The defining attribute of all phage display libraries is the physical linking of antibody phenotype (specificity and affinity) with genotype (sequence) via the phage particle-this allows for in vitro selection on immobilized antigen or whole cells (Fig. 2) . In particular, a phage library constructed with the heavy chain CDR3 enriched for basic residues to improve binding to negatively charged carbohydrates produced anti-carbohydrate antibodies that had relatively high affinity (K D ≈ 50 nM) and excellent specificity (40) . Phage display is also likely to remain useful for discovery of antibodies against non-protein targets, evolution of dual-binding antibodies and for affinity maturation, due to the limitations of the natural immune system. Comparison of the efficiency of antibody selection from semi-synthetic scFv and non-immune Fab phage display libraries against protein targets for rapid development of diagnostic immunoassays The human combinatorial antibody library HuCAL GOLD combines diversification of all six CDRs according to the natural immune system with a novel display method for efficient selection of high-affinity antibodies	./cache/cord-335311-l73hsik0.txt	./txt/cord-335311-l73hsik0.txt