id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-307320-fxs31d66	Ubah, Obinna	Monoclonal Antibodies and Antibody Like Fragments Derived from Immunised Phage Display Libraries	2018-03-17		.txt	text/plain	8292	365	30	However, by combining the power of immunisation with phage display, several high affinity monoclonal antibodies against "difficult" antigenic targets have been isolated from relative small antibody libraries and where traditional approaches have failed [33, 98] . The above study demonstrates the power of animal immunisation and phage display based selection strategies to isolate high affinity monoclonal antibodies towards non-antigenic targets which inherently lack properties like aromaticity and charge. Spleen samples from mice immunised with gamma inactivated Brucella melitensis strain 16 M bacteria was used to construct a phage display library and isolate monoclonal antibody fragments that specifically recognise Brucella species. Similarly high affinity neutralising antibody fragments against the protective antigen (PA) of anthrax toxin was isolated from a Macaca immunised phage display library. Lymphocytes from the bone marrow cells of two chimpanzees immunised with anthrax toxin PA, LF and Edema factor (EF) were used to construct scFv phage display libraries and neutralising antibodies were isolated against PA and LF proteins.	./cache/cord-307320-fxs31d66.txt	./txt/cord-307320-fxs31d66.txt