id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-298910-2601n7a9	Leu, Sy-Jye	Generation and characterization of anti-α-enolase single-chain antibodies in chicken	2010-10-15		.txt	text/plain	5157	278	48	The expressed heavy and light chain variable genes (VH and VL) were isolated from spleen B cells and amplified to construct phage antibody libraries containing scFv molecules. After four rounds of panning selection, the scFv antibodies of randomly chosen clones were expressed and their binding specificity to α-enolase protein was verified using competitive ELISA, flow cytometry and immunofluorescence staining. Accordingly, in the present study, we generated and characterized the anti-␣-enolase polyclonal IgY antibodies in chicken and monoclonal scFv antibodies by a phage display system using ELISA, Western blotting, flow cytometry and immunofluorescence staining. EnL2 and EnL5 scFv antibodies purified as a single band on SDS-PAGE (data not shown) were able to detect ␣-enolase protein in PE089 cells, and the binding signal is comparable to that of commercially available rabbit polyclonal antibodies specific for ␣-enolase as demonstrated in Fig. 7 .	./cache/cord-298910-2601n7a9.txt	./txt/cord-298910-2601n7a9.txt