id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-296928-wu14k7u9	Hofmann, Tim	Greatest Hits—Innovative Technologies for High Throughput Identification of Bispecific Antibodies	2020-09-08		.txt	text/plain	7388	326	29	In this review, we provide an overview of the most relevant methodologies for bispecific screening purposes—the DuoBody concept, paired light chain single cell production approaches, Sortase A and Transglutaminase, the SpyTag/SpyCatcher system, and inteins—and elaborate on the benefits as well as drawbacks of the different technologies. In order to overcome this bottleneck, technologies such as controlled Fab-arm exchange used for DuoBodies [22] , paired light chain single cell production approaches [23] , microbial transglutaminase [24] or Sortase A [25] mediated bioconjugation, the SpyTag/SpyCatcher system [26, 27] , or split inteins [28] were adapted to enable broad bispecific antibody screening ( Figure 1 ). In order to overcome this bottleneck, technologies such as controlled Fab-arm exchange used for DuoBodies [22] , paired light chain single cell production approaches [23] , microbial transglutaminase [24] or Sortase A [25] mediated bioconjugation, the SpyTag/SpyCatcher system [26, 27] , or split inteins [28] were adapted to enable broad bispecific antibody screening ( Figure 1 ).	./cache/cord-296928-wu14k7u9.txt	./txt/cord-296928-wu14k7u9.txt