id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-267736-rya9w6sh	Kang, Xiaoping	Development of an ELISA-array for simultaneous detection of five encephalitis viruses	2012-02-27		.txt	text/plain	2990	178	47	The ELISA-array assay is based on a "sandwich" ELISA format and consists of viral antibodies printed directly on 96-well microtiter plates, allowing for direct detection of 5 viruses. The developed ELISA-array proved to have similar specificity and higher sensitivity compared with the conventional ELISAs. This method was validated by different viral cultures and three chicken eggs inoculated with infected patient serum. The results demonstrated that this method combined the advantage of ELISA and protein array (multiplex and ease of use) and has potential for the identification of clinical encephalitis virus. When spotting, different spotting buffers and concentrations of capture monoclonal antibodies were evaluated to optimize the ELISA-array assay. To verify the results tested by ELISA-array, RNA extracted from chicken eggs was applied to a real time-RT-PCR assay using primers and probes targeting TBEV. In this study, we developed a multiplex ELISA-based method in a double-antibody sandwich format for the simultaneous detection of five encephalitis-associated viral pathogens.	./cache/cord-267736-rya9w6sh.txt	./txt/cord-267736-rya9w6sh.txt