id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-255936-hfa9w2dg	Jin, Junyeong	The level of decoy epitope in PCV2 vaccine affects the neutralizing activity of sera in the immunized animals	2018-02-12		.txt	text/plain	3479	208	47	In this study, we tested this hypothesis by generating an antibody specific to the decoy epitope on the capsid protein of porcine circovirus type 2 (PCV2). To monitor the reactivity of sera to the CP 169e180 epitope, enzyme immunoassays, using BSA-conjugated CP 169e180 peptides and HRPconjugated anti-guinea pig IgG antibodies (Bethyl Laboratories), were performed as described previously. In a competition enzyme immunoassay employing a microtiter plate coated with BSA-conjugated CP 169e180 peptide and HRP-conjugated anti-porcine IgG antibody, the recombinant anti-CP 169e180 antibody almost completely inhibited the binding of naturally occurring anti-CP 169e180 antibody to the peptide in a PCV2-infected pig's sera (Fig. 2) . In an enzyme immunoassay employing a microtiter plate coated with BSA-conjugated CP 169e180 peptide, sera from animals immunized with vaccine A had a significantly higher antibody titer than vaccine B-vaccinated animals (p < 0.01) (Fig. 3D) .	./cache/cord-255936-hfa9w2dg.txt	./txt/cord-255936-hfa9w2dg.txt