id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-048360-n9sih438	Villard, Viviane	Rapid Identification of Malaria Vaccine Candidates Based on α-Helical Coiled Coil Protein Motif	2007-07-25		.txt	text/plain	4794	228	45	To identify malaria antigens for vaccine development, we selected α-helical coiled coil domains of proteins predicted to be present in the parasite erythrocytic stage. Here, we focus on the Pf parasite erythrocytic stage, a target of protective antibodies and describe a straightforward, rapid procedure based on bioinformatic analysis of a-helical coiled-coil motifs and peptide synthesis. With regard to the structural features and cellular location prediction of the proteins corresponding to the peptides selected for ADCI assays ( Table 1) , 15 of the proteins contain a pentapeptide conforming to the PEXEL consensus [19, 20; 21, 22] , but that none of these have a position within the amino acid sequence that conforms to the location of known active PEXEL motifs (see Materials and Methods and membrane segments, and none of them has a GPI anchor. In conclusion, an approach combining a genome-wide search by bioinformatics of a-helical coiled coil protein motifs and chemical synthesis can lead to the rapid identification and development of new malaria vaccine candidates.	./cache/cord-048360-n9sih438.txt	./txt/cord-048360-n9sih438.txt