id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-032598-i0jm3p1s	Hu, Jing	Direct imaging of antigen–antibody binding by atomic force microscopy	2020-09-24		.txt	text/plain	3133	170	45	In this study, the morphology of biotinylated antibody-specific Immunoglobulin E (IgE) immune complexes has been successfully imaged by atomic force microscopy (AFM) in the tapping-mode. The AFM images indicated that the individual immune complex was composed of an IgE and a biotinylated antibody. To the best of our knowledge, the biotinylated antibody-specific IgE immune complexes imaged by AFM have never been reported. Meanwhile, Fig. 6d , h and l would represent the biotinylated antibody-specific IgE immune complex adsorbed on the mica surface by the IgE fragment and the two Fab fragments of biotinylated antibody. In this paper, the morphology and length of IgE, biotinylated antibodies and biotinylated antibody-specific IgE immune complexes were analyzed by AFM, respectively. The results of AFM imaging demonstrated that the immune complexes exhibited various morphologies, and we were able to identify the IgE and biotinylated antibody based on the protein morphology in most cases.	./cache/cord-032598-i0jm3p1s.txt	./txt/cord-032598-i0jm3p1s.txt