id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-310058-tp42rgmv	Zhu, Yuan-Shou	Multiplex and visual detection of African Swine Fever Virus (ASFV) based on Hive-Chip and direct loop-mediated isothermal amplification	2020-10-08		.txt	text/plain	5608	287	55	title: Multiplex and visual detection of African Swine Fever Virus (ASFV) based on Hive-Chip and direct loop-mediated isothermal amplification On-chip LAMP showed the limits of detection (LOD) of ASFV synthetic DNAs and mock samples are 30 and 50 copies per microliter, respectively, and there is no cross-reaction among the target genes. Multiplex detection of real samples of ASFV and other swine viruses further demonstrates the high sensitivity and specificity of Hive-Chip. Last but not least, all methods aforementioned are usually focused on detecting only one gene target at a time, such as B646L encoding viral protein P72 (VP72) [24] [25] [26] [27] [28] [29] , 9GL encoding sulfhydryl oxidase [20] , P1192R encoding topoisomerase II [22] , or K78R encoding DNA binding protein P10 [23] , which may lead to false-negative results because of possible mutations on the complex, highly variable genomes of ASFV.	./cache/cord-310058-tp42rgmv.txt	./txt/cord-310058-tp42rgmv.txt