id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-277541-ieex2xwx	Sun, Jian	Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv “White Cloud Bean”	2010-06-14		.txt	text/plain	3588	210	46	title: Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv "White Cloud Bean" A purification protocol that comprised ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose, and gel filtration by FPLC on Superdex 75 was complied to isolate two trypsin inhibitors from Phaseolus vulgaris cv "White Cloud Bean". They were lacking in activity toward lymphoma MBL2 cells and inhibitory effect on HIV-1 reverse transcriptase and fungal growth when tested up to 100 μM. The objective of the present study was to isolate and characterize proteins with protease inhibitory activity from white cloud beans. The present study disclosed the production of two trypsin inhibitors with closely related N-terminal sequences chromatographic behavior and bioactivities by the white cloud bean variety of Phaseolus vulgaris. White cloud bean trypsin inhibitors demonstrate antiproliferative activity against tumor cells but do no inhibit mycelial growth or HIV-1 reverse transcriptase.	./cache/cord-277541-ieex2xwx.txt	./txt/cord-277541-ieex2xwx.txt