id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-258239-7xyqlz0u	Gärtner, Fabian	Application of an Activity-Based Probe to Determine Proteolytic Activity of Cell Surface Cathepsin G by Mass Cytometry Data Acquisition	2020-10-19		.txt	text/plain	2548	141	51	To this end, we established an approach to determine the proteolytic activity of CatG on the cell surface of neutrophils, NK cells, B cells, and T cells in peripheral blood mononuclear cells (PBMCs) by combining the activity-based probe MARS116-Bt and the antibiotin-150 Nd antibody with CyTOF analysis. Therefore, MARS116-Bt-anti-biotin-150 Nd antibodies are useful to profile a vast panel of different cell subsets in order to evaluate catalytically active CatG, its regulation, or inhibition on the cell surface. The next-generation flow cytometry CyTOF setup in combination with MARS116-Bt-anti-biotin-150 Nd antibodies allows us to analyze and gate distinct cell subsets and simultaneously determine the proteolytic activity of CatG. Furthermore, MARS116-Bt-anti-biotin-150 Nd antibodies can be added to a vast panel of activation markers in CyTOF analysis to characterize immune cells during inflammation, inducing a pathologic effect, in homeostasis, or when stimulated with different substances.	./cache/cord-258239-7xyqlz0u.txt	./txt/cord-258239-7xyqlz0u.txt