id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000884-zq8kqf6h	Shen, Hsin-Hui	Reconstitution of Membrane Proteins into Model Membranes: Seeking Better Ways to Retain Protein Activities	2013-01-14		.txt	text/plain	6861	355	39	This review will discuss the reconstitution of membrane protein activities in four different types of model membrane—monolayers, supported lipid bilayers, liposomes and nanodiscs, comparing their advantages in membrane protein reconstitution. To simplify cell membrane systems, model membranes such as monolayers, bilayers, liposomes and nanodiscs have been developed, enabling detailed investigation of membrane protein structure in lipid membranes. There are a number of approaches used to create a model membrane in order to mimic properties of the native cell membrane, and we will review these various approaches for reconstituting membrane proteins into different types of model membrane-monolayers [5] , supported planar lipid bilayer [6] and liposomes [7] as shown in Figure 1A -C. By using this approach, it appears that both PLA2 and PLC are active at the monolayer model membrane, indicating that the kinetics of phospholipid hydrolysis at the air-water interface can be monitored by biophysical characterization techniques in situ such as PM-IRRAS and infrared reflection adsorption spectroscopy [22] .	./cache/cord-000884-zq8kqf6h.txt	./txt/cord-000884-zq8kqf6h.txt