id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-346245-o9hvuwvq	Harvey, David J.	Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2009–2010	2014-05-26		.txt	text/plain	62449	3625	40	Advantages and drawbacks of this approach are critically (Song et al., 2009d) Bacillus anthracis tetrasaccharide with thiol linker MALDI For attachment to a maleimide functionalized Microarray to study of carbohydrate-antibody interactions (Oberli et al., 2010) Glycodendrimers with N 3 group terminating in α-Man, β-GlcNAc or β-Gal TOF Immobilized on an acetylenyl-terminated gold substrate via click chemistry High-mannose glycans -Oxime linked TOF Used to probe binding to malectin MUC1 Glycopeptides TOF Synthesis on an amine-reactive hydrogelcoated microarray glass surface. The non-commercial CGE-on-a-chip assay allowed electrophoretic separation of proteins in the MW range from 14 kDa to 1 MDa. MW assignment was limited to 500 kDa in the case of SDS-PAGE but with the proper matrix (THAP for most glycoproteins, sinapinic acid for a2-macroglobulin) and sample preparation, analysis with a standard MALDI-TOF-MS provided accurate MWs for all high MW proteins up to 1 MDa. Three methods for N-glycan characterization, namely MALDI-MS of glycopeptides from tryptic digestion, negativeion ESI-MS/MS of released N-glycans, and normal-phase HPLC of fluorescently labeled glycans, in combination with exoglycosidase sequencing, have been evaluated for glycan identification using monoclonal antibodies expressed in tobacco plants as model compounds (Triguero et al., 2010) .	./cache/cord-346245-o9hvuwvq.txt	./txt/cord-346245-o9hvuwvq.txt