id sid tid token lemma pos cord-013178-li1x1m25 1 1 key key JJ cord-013178-li1x1m25 1 2 : : : cord-013178-li1x1m25 1 3 cord-013178-li1x1m25 cord-013178-li1x1m25 NNP cord-013178-li1x1m25 1 4 authors author NNS cord-013178-li1x1m25 1 5 : : : cord-013178-li1x1m25 2 1 Hung hang VBN cord-013178-li1x1m25 3 1 , , , cord-013178-li1x1m25 3 2 Ling Ling NNP cord-013178-li1x1m25 3 3 - - HYPH cord-013178-li1x1m25 3 4 Chu Chu NNP cord-013178-li1x1m25 3 5 title title NN cord-013178-li1x1m25 3 6 : : : cord-013178-li1x1m25 4 1 The the DT cord-013178-li1x1m25 4 2 Monoclonal Monoclonal NNP cord-013178-li1x1m25 4 3 Antibody Antibody NNP cord-013178-li1x1m25 4 4 Recognized recognize VBD cord-013178-li1x1m25 4 5 the the DT cord-013178-li1x1m25 4 6 Open open JJ cord-013178-li1x1m25 4 7 Reading reading NN cord-013178-li1x1m25 4 8 Frame Frame NNP cord-013178-li1x1m25 4 9 Protein Protein NNP cord-013178-li1x1m25 4 10 in in IN cord-013178-li1x1m25 4 11 Porcine Porcine NNP cord-013178-li1x1m25 4 12 Circovirus Circovirus NNP cord-013178-li1x1m25 4 13 Type Type NNP cord-013178-li1x1m25 4 14 2-Infected 2-Infected NNP cord-013178-li1x1m25 4 15 Peripheral Peripheral NNP cord-013178-li1x1m25 4 16 Blood Blood NNP cord-013178-li1x1m25 4 17 Mononuclear Mononuclear NNP cord-013178-li1x1m25 4 18 Cells Cells NNP cord-013178-li1x1m25 4 19 date date NN cord-013178-li1x1m25 4 20 : : : cord-013178-li1x1m25 4 21 2020 2020 CD cord-013178-li1x1m25 4 22 - - HYPH cord-013178-li1x1m25 4 23 08 08 CD cord-013178-li1x1m25 4 24 - - SYM cord-013178-li1x1m25 4 25 29 29 CD cord-013178-li1x1m25 4 26 journal journal NN cord-013178-li1x1m25 4 27 : : : cord-013178-li1x1m25 5 1 Viruses virus NNS cord-013178-li1x1m25 5 2 DOI DOI NNP cord-013178-li1x1m25 5 3 : : : cord-013178-li1x1m25 5 4 10.3390 10.3390 CD cord-013178-li1x1m25 5 5 / / SYM cord-013178-li1x1m25 5 6 v12090961 v12090961 VB cord-013178-li1x1m25 5 7 sha sha NNP cord-013178-li1x1m25 5 8 : : : cord-013178-li1x1m25 5 9 939e0611f01559978804290a17c60b95d4209ef0 939e0611f01559978804290a17c60b95d4209ef0 CD cord-013178-li1x1m25 5 10 doc_id doc_id CD cord-013178-li1x1m25 5 11 : : : cord-013178-li1x1m25 5 12 13178 13178 CD cord-013178-li1x1m25 5 13 cord_uid cord_uid NNS cord-013178-li1x1m25 5 14 : : : cord-013178-li1x1m25 6 1 li1x1m25 li1x1m25 NFP cord-013178-li1x1m25 6 2 The the DT cord-013178-li1x1m25 6 3 purpose purpose NN cord-013178-li1x1m25 6 4 of of IN cord-013178-li1x1m25 6 5 this this DT cord-013178-li1x1m25 6 6 study study NN cord-013178-li1x1m25 6 7 in in IN cord-013178-li1x1m25 6 8 the the DT cord-013178-li1x1m25 6 9 context context NN cord-013178-li1x1m25 6 10 of of IN cord-013178-li1x1m25 6 11 the the DT cord-013178-li1x1m25 6 12 open open JJ cord-013178-li1x1m25 6 13 reading reading NN cord-013178-li1x1m25 6 14 frame frame NN cord-013178-li1x1m25 6 15 3 3 CD cord-013178-li1x1m25 7 1 ( ( -LRB- cord-013178-li1x1m25 7 2 ORF3 ORF3 NNP cord-013178-li1x1m25 7 3 ) ) -RRB- cord-013178-li1x1m25 7 4 protein protein NN cord-013178-li1x1m25 7 5 of of IN cord-013178-li1x1m25 7 6 porcine porcine JJ cord-013178-li1x1m25 7 7 circovirus circovirus NNP cord-013178-li1x1m25 7 8 type type NN cord-013178-li1x1m25 7 9 2 2 CD cord-013178-li1x1m25 7 10 ( ( -LRB- cord-013178-li1x1m25 7 11 PCV2 PCV2 NNP cord-013178-li1x1m25 7 12 ) ) -RRB- cord-013178-li1x1m25 7 13 was be VBD cord-013178-li1x1m25 7 14 especially especially RB cord-013178-li1x1m25 7 15 its -PRON- PRP$ cord-013178-li1x1m25 7 16 location location NN cord-013178-li1x1m25 7 17 and and CC cord-013178-li1x1m25 7 18 its -PRON- PRP$ cord-013178-li1x1m25 7 19 relation relation NN cord-013178-li1x1m25 7 20 to to IN cord-013178-li1x1m25 7 21 the the DT cord-013178-li1x1m25 7 22 capsid capsid JJ cord-013178-li1x1m25 7 23 protein protein NN cord-013178-li1x1m25 7 24 and and CC cord-013178-li1x1m25 7 25 the the DT cord-013178-li1x1m25 7 26 apoptosis apoptosis NN cord-013178-li1x1m25 7 27 protein protein NN cord-013178-li1x1m25 7 28 in in IN cord-013178-li1x1m25 7 29 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 7 30 porcine porcine JJ cord-013178-li1x1m25 7 31 peripheral peripheral JJ cord-013178-li1x1m25 7 32 blood blood NN cord-013178-li1x1m25 7 33 mononuclear mononuclear NN cord-013178-li1x1m25 7 34 cells cell NNS cord-013178-li1x1m25 7 35 ( ( -LRB- cord-013178-li1x1m25 7 36 PBMCs pbmc NNS cord-013178-li1x1m25 7 37 ) ) -RRB- cord-013178-li1x1m25 7 38 . . . cord-013178-li1x1m25 8 1 To to TO cord-013178-li1x1m25 8 2 detect detect VB cord-013178-li1x1m25 8 3 the the DT cord-013178-li1x1m25 8 4 ORF3 ORF3 NNP cord-013178-li1x1m25 8 5 protein protein NN cord-013178-li1x1m25 8 6 , , , cord-013178-li1x1m25 8 7 monoclonal monoclonal JJ cord-013178-li1x1m25 8 8 antibodies antibody NNS cord-013178-li1x1m25 8 9 ( ( -LRB- cord-013178-li1x1m25 8 10 mAbs mAbs NNP cord-013178-li1x1m25 8 11 ) ) -RRB- cord-013178-li1x1m25 8 12 were be VBD cord-013178-li1x1m25 8 13 generated generate VBN cord-013178-li1x1m25 8 14 in in IN cord-013178-li1x1m25 8 15 this this DT cord-013178-li1x1m25 8 16 study study NN cord-013178-li1x1m25 8 17 . . . cord-013178-li1x1m25 9 1 The the DT cord-013178-li1x1m25 9 2 mAb mAb NNP cord-013178-li1x1m25 9 3 7D3 7D3 NNP cord-013178-li1x1m25 9 4 binds bind VBZ cord-013178-li1x1m25 9 5 to to IN cord-013178-li1x1m25 9 6 the the DT cord-013178-li1x1m25 9 7 ORF3 ORF3 NNP cord-013178-li1x1m25 9 8 peptide peptide NN cord-013178-li1x1m25 9 9 ( ( -LRB- cord-013178-li1x1m25 9 10 residues residue NNS cord-013178-li1x1m25 9 11 35–66 35–66 NNP cord-013178-li1x1m25 9 12 ) ) -RRB- cord-013178-li1x1m25 9 13 and and CC cord-013178-li1x1m25 9 14 the the DT cord-013178-li1x1m25 9 15 native native JJ cord-013178-li1x1m25 9 16 ORF3 ORF3 NNP cord-013178-li1x1m25 9 17 protein protein NN cord-013178-li1x1m25 9 18 in in IN cord-013178-li1x1m25 9 19 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 9 20 PBMCs pbmc NNS cord-013178-li1x1m25 9 21 , , , cord-013178-li1x1m25 9 22 as as IN cord-013178-li1x1m25 9 23 shown show VBN cord-013178-li1x1m25 9 24 by by IN cord-013178-li1x1m25 9 25 immunofluorescence immunofluorescence NN cord-013178-li1x1m25 9 26 assay assay NN cord-013178-li1x1m25 9 27 ( ( -LRB- cord-013178-li1x1m25 9 28 IFA IFA NNP cord-013178-li1x1m25 9 29 ) ) -RRB- cord-013178-li1x1m25 9 30 . . . cord-013178-li1x1m25 10 1 The the DT cord-013178-li1x1m25 10 2 data datum NNS cord-013178-li1x1m25 10 3 show show VBP cord-013178-li1x1m25 10 4 that that IN cord-013178-li1x1m25 10 5 3–5 3–5 CD cord-013178-li1x1m25 10 6 % % NN cord-013178-li1x1m25 10 7 of of IN cord-013178-li1x1m25 10 8 PBMCs pbmc NNS cord-013178-li1x1m25 10 9 were be VBD cord-013178-li1x1m25 10 10 positive positive JJ cord-013178-li1x1m25 10 11 for for IN cord-013178-li1x1m25 10 12 ORF3 ORF3 NNP cord-013178-li1x1m25 10 13 protein protein NN cord-013178-li1x1m25 10 14 or or CC cord-013178-li1x1m25 10 15 p53 p53 NN cord-013178-li1x1m25 10 16 protein protein NN cord-013178-li1x1m25 10 17 . . . cord-013178-li1x1m25 11 1 Further further RB cord-013178-li1x1m25 11 2 , , , cord-013178-li1x1m25 11 3 78–82 78–82 CD cord-013178-li1x1m25 11 4 % % NN cord-013178-li1x1m25 11 5 of of IN cord-013178-li1x1m25 11 6 PBMCs pbmc NNS cord-013178-li1x1m25 11 7 were be VBD cord-013178-li1x1m25 11 8 positive positive JJ cord-013178-li1x1m25 11 9 for for IN cord-013178-li1x1m25 11 10 the the DT cord-013178-li1x1m25 11 11 capsid capsid NN cord-013178-li1x1m25 11 12 . . . cord-013178-li1x1m25 12 1 This this DT cord-013178-li1x1m25 12 2 study study NN cord-013178-li1x1m25 12 3 confirmed confirm VBD cord-013178-li1x1m25 12 4 the the DT cord-013178-li1x1m25 12 5 ORF3 ORF3 NNP cord-013178-li1x1m25 12 6 protein protein NN cord-013178-li1x1m25 12 7 not not RB cord-013178-li1x1m25 12 8 only only RB cord-013178-li1x1m25 12 9 colocalized colocalize VBN cord-013178-li1x1m25 12 10 with with IN cord-013178-li1x1m25 12 11 the the DT cord-013178-li1x1m25 12 12 capsid capsid NN cord-013178-li1x1m25 12 13 protein protein NN cord-013178-li1x1m25 12 14 but but CC cord-013178-li1x1m25 12 15 also also RB cord-013178-li1x1m25 12 16 colocalized colocalize VBN cord-013178-li1x1m25 12 17 with with IN cord-013178-li1x1m25 12 18 the the DT cord-013178-li1x1m25 12 19 p53 p53 NN cord-013178-li1x1m25 12 20 protein protein NN cord-013178-li1x1m25 12 21 in in IN cord-013178-li1x1m25 12 22 PBMCs PBMCs NNP cord-013178-li1x1m25 12 23 . . . cord-013178-li1x1m25 13 1 Immunoassays Immunoassays NNP cord-013178-li1x1m25 13 2 were be VBD cord-013178-li1x1m25 13 3 conducted conduct VBN cord-013178-li1x1m25 13 4 in in IN cord-013178-li1x1m25 13 5 this this DT cord-013178-li1x1m25 13 6 study study NN cord-013178-li1x1m25 13 7 to to TO cord-013178-li1x1m25 13 8 detect detect VB cord-013178-li1x1m25 13 9 the the DT cord-013178-li1x1m25 13 10 capsid capsid NN cord-013178-li1x1m25 13 11 protein protein NN cord-013178-li1x1m25 13 12 , , , cord-013178-li1x1m25 13 13 the the DT cord-013178-li1x1m25 13 14 ORF3 ORF3 NNP cord-013178-li1x1m25 13 15 protein protein NN cord-013178-li1x1m25 13 16 , , , cord-013178-li1x1m25 13 17 anti anti JJ cord-013178-li1x1m25 13 18 - - JJ cord-013178-li1x1m25 13 19 capsid capsid JJ cord-013178-li1x1m25 13 20 IgG igg NN cord-013178-li1x1m25 13 21 , , , cord-013178-li1x1m25 13 22 and and CC cord-013178-li1x1m25 13 23 anti anti NNP cord-013178-li1x1m25 13 24 - - NNP cord-013178-li1x1m25 13 25 ORF3 ORF3 NNP cord-013178-li1x1m25 13 26 IgG. IgG. NNP cord-013178-li1x1m25 14 1 The the DT cord-013178-li1x1m25 14 2 data datum NNS cord-013178-li1x1m25 14 3 show show VBP cord-013178-li1x1m25 14 4 the the DT cord-013178-li1x1m25 14 5 correlation correlation NN cord-013178-li1x1m25 14 6 ( ( -LRB- cord-013178-li1x1m25 14 7 r r NN cord-013178-li1x1m25 14 8 = = SYM cord-013178-li1x1m25 14 9 0.758 0.758 CD cord-013178-li1x1m25 14 10 ) ) -RRB- cord-013178-li1x1m25 14 11 of of IN cord-013178-li1x1m25 14 12 the the DT cord-013178-li1x1m25 14 13 ORF3 ORF3 NNP cord-013178-li1x1m25 14 14 protein protein NN cord-013178-li1x1m25 14 15 and and CC cord-013178-li1x1m25 14 16 the the DT cord-013178-li1x1m25 14 17 capsid capsid NN cord-013178-li1x1m25 14 18 protein protein NN cord-013178-li1x1m25 14 19 in in IN cord-013178-li1x1m25 14 20 the the DT cord-013178-li1x1m25 14 21 blood blood NN cord-013178-li1x1m25 14 22 samples sample NNS cord-013178-li1x1m25 14 23 from from IN cord-013178-li1x1m25 14 24 the the DT cord-013178-li1x1m25 14 25 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 14 26 herd herd NN cord-013178-li1x1m25 14 27 . . . cord-013178-li1x1m25 15 1 However however RB cord-013178-li1x1m25 15 2 , , , cord-013178-li1x1m25 15 3 each each DT cord-013178-li1x1m25 15 4 anti anti JJ cord-013178-li1x1m25 15 5 - - JJ cord-013178-li1x1m25 15 6 viral viral JJ cord-013178-li1x1m25 15 7 protein protein NN cord-013178-li1x1m25 15 8 IgG IgG NNP cord-013178-li1x1m25 15 9 had have VBD cord-013178-li1x1m25 15 10 a a DT cord-013178-li1x1m25 15 11 different different JJ cord-013178-li1x1m25 15 12 curve curve NN cord-013178-li1x1m25 15 13 of of IN cord-013178-li1x1m25 15 14 the the DT cord-013178-li1x1m25 15 15 profile profile NN cord-013178-li1x1m25 15 16 in in IN cord-013178-li1x1m25 15 17 the the DT cord-013178-li1x1m25 15 18 same same JJ cord-013178-li1x1m25 15 19 herd herd NN cord-013178-li1x1m25 15 20 after after IN cord-013178-li1x1m25 15 21 vaccination vaccination NN cord-013178-li1x1m25 15 22 . . . cord-013178-li1x1m25 16 1 Overall overall RB cord-013178-li1x1m25 16 2 , , , cord-013178-li1x1m25 16 3 this this DT cord-013178-li1x1m25 16 4 study study NN cord-013178-li1x1m25 16 5 provides provide VBZ cord-013178-li1x1m25 16 6 a a DT cord-013178-li1x1m25 16 7 blueprint blueprint NN cord-013178-li1x1m25 16 8 to to TO cord-013178-li1x1m25 16 9 explore explore VB cord-013178-li1x1m25 16 10 the the DT cord-013178-li1x1m25 16 11 ORF3 ORF3 NNP cord-013178-li1x1m25 16 12 protein protein NN cord-013178-li1x1m25 16 13 in in IN cord-013178-li1x1m25 16 14 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 16 15 PBMCs pbmc NNS cord-013178-li1x1m25 16 16 . . . cord-013178-li1x1m25 17 1 The the DT cord-013178-li1x1m25 17 2 Porcine porcine JJ cord-013178-li1x1m25 17 3 circovirus circovirus NN cord-013178-li1x1m25 17 4 ( ( -LRB- cord-013178-li1x1m25 17 5 PCV PCV NNP cord-013178-li1x1m25 17 6 ) ) -RRB- cord-013178-li1x1m25 17 7 is be VBZ cord-013178-li1x1m25 17 8 a a DT cord-013178-li1x1m25 17 9 small small JJ cord-013178-li1x1m25 17 10 virus virus NN cord-013178-li1x1m25 17 11 and and CC cord-013178-li1x1m25 17 12 contains contain VBZ cord-013178-li1x1m25 17 13 closed closed JJ cord-013178-li1x1m25 17 14 circular circular JJ cord-013178-li1x1m25 17 15 single single RB cord-013178-li1x1m25 17 16 - - HYPH cord-013178-li1x1m25 17 17 stranded strand VBN cord-013178-li1x1m25 17 18 DNA dna NN cord-013178-li1x1m25 17 19 [ [ -LRB- cord-013178-li1x1m25 17 20 1 1 CD cord-013178-li1x1m25 17 21 ] ] -RRB- cord-013178-li1x1m25 17 22 . . . cord-013178-li1x1m25 18 1 Previous previous JJ cord-013178-li1x1m25 18 2 studies study NNS cord-013178-li1x1m25 18 3 indicated indicate VBD cord-013178-li1x1m25 18 4 that that IN cord-013178-li1x1m25 18 5 porcine porcine JJ cord-013178-li1x1m25 18 6 circovirus circovirus NN cord-013178-li1x1m25 18 7 type type NN cord-013178-li1x1m25 18 8 1 1 CD cord-013178-li1x1m25 18 9 ( ( -LRB- cord-013178-li1x1m25 18 10 PCV1 PCV1 NNP cord-013178-li1x1m25 18 11 ) ) -RRB- cord-013178-li1x1m25 18 12 is be VBZ cord-013178-li1x1m25 18 13 non non JJ cord-013178-li1x1m25 18 14 - - JJ cord-013178-li1x1m25 18 15 pathogenic pathogenic JJ cord-013178-li1x1m25 18 16 to to IN cord-013178-li1x1m25 18 17 porcine porcine JJ cord-013178-li1x1m25 18 18 herd herd NN cord-013178-li1x1m25 18 19 [ [ -LRB- cord-013178-li1x1m25 18 20 2 2 CD cord-013178-li1x1m25 18 21 , , , cord-013178-li1x1m25 18 22 3 3 CD cord-013178-li1x1m25 18 23 ] ] -RRB- cord-013178-li1x1m25 18 24 . . . cord-013178-li1x1m25 19 1 However however RB cord-013178-li1x1m25 19 2 , , , cord-013178-li1x1m25 19 3 porcine porcine JJ cord-013178-li1x1m25 19 4 circovirus circovirus NN cord-013178-li1x1m25 19 5 type type NN cord-013178-li1x1m25 19 6 2 2 CD cord-013178-li1x1m25 19 7 ( ( -LRB- cord-013178-li1x1m25 19 8 PCV2)-infected pcv2)-infecte VBN cord-013178-li1x1m25 19 9 pigs pig NNS cord-013178-li1x1m25 19 10 showed show VBD cord-013178-li1x1m25 19 11 dullness dullness NN cord-013178-li1x1m25 19 12 , , , cord-013178-li1x1m25 19 13 thinness thinness NN cord-013178-li1x1m25 19 14 , , , cord-013178-li1x1m25 19 15 lymphadenopathy lymphadenopathy JJ cord-013178-li1x1m25 19 16 , , , cord-013178-li1x1m25 19 17 jaundice jaundice NN cord-013178-li1x1m25 19 18 , , , cord-013178-li1x1m25 19 19 hepatomegaly hepatomegaly NNP cord-013178-li1x1m25 19 20 , , , cord-013178-li1x1m25 19 21 and and CC cord-013178-li1x1m25 19 22 other other JJ cord-013178-li1x1m25 19 23 manifestations manifestation NNS cord-013178-li1x1m25 19 24 [ [ -LRB- cord-013178-li1x1m25 19 25 4 4 CD cord-013178-li1x1m25 19 26 ] ] -RRB- cord-013178-li1x1m25 19 27 [ [ -LRB- cord-013178-li1x1m25 19 28 5 5 CD cord-013178-li1x1m25 19 29 ] ] -RRB- cord-013178-li1x1m25 19 30 [ [ -LRB- cord-013178-li1x1m25 19 31 6 6 CD cord-013178-li1x1m25 19 32 ] ] -RRB- cord-013178-li1x1m25 19 33 [ [ -LRB- cord-013178-li1x1m25 19 34 7 7 CD cord-013178-li1x1m25 19 35 ] ] -RRB- cord-013178-li1x1m25 19 36 [ [ -LRB- cord-013178-li1x1m25 19 37 8 8 CD cord-013178-li1x1m25 19 38 ] ] -RRB- cord-013178-li1x1m25 19 39 . . . cord-013178-li1x1m25 20 1 Lymphocyte lymphocyte NN cord-013178-li1x1m25 20 2 depletion depletion NN cord-013178-li1x1m25 20 3 and and CC cord-013178-li1x1m25 20 4 apoptosis apoptosis NN cord-013178-li1x1m25 20 5 of of IN cord-013178-li1x1m25 20 6 lymphocytes lymphocyte NNS cord-013178-li1x1m25 20 7 were be VBD cord-013178-li1x1m25 20 8 the the DT cord-013178-li1x1m25 20 9 significant significant JJ cord-013178-li1x1m25 20 10 histopathological histopathological JJ cord-013178-li1x1m25 20 11 lesions lesion NNS cord-013178-li1x1m25 20 12 in in IN cord-013178-li1x1m25 20 13 lymphoid lymphoid JJ cord-013178-li1x1m25 20 14 tissues tissue NNS cord-013178-li1x1m25 20 15 of of IN cord-013178-li1x1m25 20 16 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 20 17 pigs pig NNS cord-013178-li1x1m25 20 18 [ [ -LRB- cord-013178-li1x1m25 20 19 9 9 CD cord-013178-li1x1m25 20 20 , , , cord-013178-li1x1m25 20 21 10 10 CD cord-013178-li1x1m25 20 22 ] ] -RRB- cord-013178-li1x1m25 20 23 . . . cord-013178-li1x1m25 21 1 PCV2 pcv2 JJ cord-013178-li1x1m25 21 2 infection infection NN cord-013178-li1x1m25 21 3 causes cause VBZ cord-013178-li1x1m25 21 4 a a DT cord-013178-li1x1m25 21 5 reduction reduction NN cord-013178-li1x1m25 21 6 of of IN cord-013178-li1x1m25 21 7 T t NN cord-013178-li1x1m25 21 8 - - HYPH cord-013178-li1x1m25 21 9 and and CC cord-013178-li1x1m25 21 10 B b NN cord-013178-li1x1m25 21 11 - - HYPH cord-013178-li1x1m25 21 12 lymphocytes lymphocyte NNS cord-013178-li1x1m25 21 13 in in IN cord-013178-li1x1m25 21 14 the the DT cord-013178-li1x1m25 21 15 PBMC PBMC NNP cord-013178-li1x1m25 21 16 [ [ -LRB- cord-013178-li1x1m25 21 17 11 11 CD cord-013178-li1x1m25 21 18 ] ] -RRB- cord-013178-li1x1m25 21 19 . . . cord-013178-li1x1m25 22 1 Likewise likewise RB cord-013178-li1x1m25 22 2 , , , cord-013178-li1x1m25 22 3 PCV2-associated pcv2-associated JJ cord-013178-li1x1m25 22 4 disease disease NN cord-013178-li1x1m25 22 5 ( ( -LRB- cord-013178-li1x1m25 22 6 PCVAD PCVAD NNP cord-013178-li1x1m25 22 7 ) ) -RRB- cord-013178-li1x1m25 22 8 manifested manifest VBD cord-013178-li1x1m25 22 9 as as IN cord-013178-li1x1m25 22 10 severe severe JJ cord-013178-li1x1m25 22 11 swine swine NN cord-013178-li1x1m25 22 12 herd herd NN cord-013178-li1x1m25 22 13 problems problem NNS cord-013178-li1x1m25 22 14 [ [ -LRB- cord-013178-li1x1m25 22 15 4 4 CD cord-013178-li1x1m25 22 16 , , , cord-013178-li1x1m25 22 17 [ [ -LRB- cord-013178-li1x1m25 22 18 6 6 CD cord-013178-li1x1m25 22 19 ] ] -RRB- cord-013178-li1x1m25 22 20 [ [ -LRB- cord-013178-li1x1m25 22 21 7 7 CD cord-013178-li1x1m25 22 22 ] ] -RRB- cord-013178-li1x1m25 22 23 [ [ -LRB- cord-013178-li1x1m25 22 24 8 8 CD cord-013178-li1x1m25 22 25 ] ] -RRB- cord-013178-li1x1m25 22 26 . . . cord-013178-li1x1m25 23 1 Recently recently RB cord-013178-li1x1m25 23 2 , , , cord-013178-li1x1m25 23 3 PCVAD PCVAD NNP cord-013178-li1x1m25 23 4 has have VBZ cord-013178-li1x1m25 23 5 become become VBN cord-013178-li1x1m25 23 6 one one CD cord-013178-li1x1m25 23 7 of of IN cord-013178-li1x1m25 23 8 the the DT cord-013178-li1x1m25 23 9 significant significant JJ cord-013178-li1x1m25 23 10 swine swine NN cord-013178-li1x1m25 23 11 diseases disease NNS cord-013178-li1x1m25 23 12 worldwide worldwide RB cord-013178-li1x1m25 23 13 , , , cord-013178-li1x1m25 23 14 and and CC cord-013178-li1x1m25 23 15 it -PRON- PRP cord-013178-li1x1m25 23 16 impacts impact VBZ cord-013178-li1x1m25 23 17 pork pork NN cord-013178-li1x1m25 23 18 production production NN cord-013178-li1x1m25 23 19 [ [ -LRB- cord-013178-li1x1m25 23 20 7 7 CD cord-013178-li1x1m25 23 21 ] ] -RRB- cord-013178-li1x1m25 23 22 . . . cord-013178-li1x1m25 24 1 At at RB cord-013178-li1x1m25 24 2 least least RBS cord-013178-li1x1m25 24 3 four four CD cord-013178-li1x1m25 24 4 commercial commercial JJ cord-013178-li1x1m25 24 5 PCV2a PCV2a NNP cord-013178-li1x1m25 24 6 vaccines vaccine NNS cord-013178-li1x1m25 24 7 have have VBP cord-013178-li1x1m25 24 8 been be VBN cord-013178-li1x1m25 24 9 widely widely RB cord-013178-li1x1m25 24 10 used use VBN cord-013178-li1x1m25 24 11 to to TO cord-013178-li1x1m25 24 12 reduce reduce VB cord-013178-li1x1m25 24 13 viremia viremia NN cord-013178-li1x1m25 24 14 and and CC cord-013178-li1x1m25 24 15 PCV2 pcv2 JJ cord-013178-li1x1m25 24 16 infectious infectious JJ cord-013178-li1x1m25 24 17 pressure pressure NN cord-013178-li1x1m25 24 18 in in IN cord-013178-li1x1m25 24 19 swine swine NN cord-013178-li1x1m25 24 20 herds herd NNS cord-013178-li1x1m25 24 21 [ [ -LRB- cord-013178-li1x1m25 24 22 12 12 CD cord-013178-li1x1m25 24 23 ] ] -RRB- cord-013178-li1x1m25 25 1 [ [ -LRB- cord-013178-li1x1m25 25 2 13 13 CD cord-013178-li1x1m25 25 3 ] ] -RRB- cord-013178-li1x1m25 25 4 [ [ -LRB- cord-013178-li1x1m25 25 5 14 14 CD cord-013178-li1x1m25 25 6 ] ] -RRB- cord-013178-li1x1m25 25 7 [ [ -LRB- cord-013178-li1x1m25 25 8 15 15 CD cord-013178-li1x1m25 25 9 ] ] -RRB- cord-013178-li1x1m25 25 10 . . . cord-013178-li1x1m25 26 1 That that DT cord-013178-li1x1m25 26 2 might may MD cord-013178-li1x1m25 26 3 have have VB cord-013178-li1x1m25 26 4 caused cause VBN cord-013178-li1x1m25 26 5 the the DT cord-013178-li1x1m25 26 6 global global JJ cord-013178-li1x1m25 26 7 trend trend NN cord-013178-li1x1m25 26 8 shift shift NN cord-013178-li1x1m25 26 9 from from IN cord-013178-li1x1m25 26 10 PCV2a PCV2a NNP cord-013178-li1x1m25 26 11 to to IN cord-013178-li1x1m25 26 12 PCV2b PCV2b NNP cord-013178-li1x1m25 26 13 first first RB cord-013178-li1x1m25 26 14 [ [ -LRB- cord-013178-li1x1m25 26 15 16 16 CD cord-013178-li1x1m25 26 16 ] ] -RRB- cord-013178-li1x1m25 26 17 [ [ -LRB- cord-013178-li1x1m25 26 18 17 17 CD cord-013178-li1x1m25 26 19 ] ] -RRB- cord-013178-li1x1m25 26 20 [ [ -LRB- cord-013178-li1x1m25 26 21 18 18 CD cord-013178-li1x1m25 26 22 ] ] -RRB- cord-013178-li1x1m25 26 23 [ [ -LRB- cord-013178-li1x1m25 26 24 19 19 CD cord-013178-li1x1m25 26 25 ] ] -RRB- cord-013178-li1x1m25 26 26 , , , cord-013178-li1x1m25 26 27 and and CC cord-013178-li1x1m25 26 28 then then RB cord-013178-li1x1m25 26 29 PCV2d PCV2d NNP cord-013178-li1x1m25 26 30 ( ( -LRB- cord-013178-li1x1m25 26 31 PCV2b PCV2b NNP cord-013178-li1x1m25 26 32 mutant mutant JJ cord-013178-li1x1m25 26 33 strain strain NN cord-013178-li1x1m25 26 34 ) ) -RRB- cord-013178-li1x1m25 26 35 has have VBZ cord-013178-li1x1m25 26 36 become become VBN cord-013178-li1x1m25 26 37 a a DT cord-013178-li1x1m25 26 38 predominant predominant JJ cord-013178-li1x1m25 26 39 genotype genotype NN cord-013178-li1x1m25 26 40 globally globally RB cord-013178-li1x1m25 26 41 [ [ -LRB- cord-013178-li1x1m25 26 42 20 20 CD cord-013178-li1x1m25 26 43 ] ] -RRB- cord-013178-li1x1m25 26 44 [ [ -LRB- cord-013178-li1x1m25 26 45 21 21 CD cord-013178-li1x1m25 26 46 ] ] -RRB- cord-013178-li1x1m25 26 47 [ [ -LRB- cord-013178-li1x1m25 26 48 22 22 CD cord-013178-li1x1m25 26 49 ] ] -RRB- cord-013178-li1x1m25 26 50 [ [ -LRB- cord-013178-li1x1m25 26 51 23 23 CD cord-013178-li1x1m25 26 52 ] ] -RRB- cord-013178-li1x1m25 26 53 [ [ -LRB- cord-013178-li1x1m25 26 54 24 24 CD cord-013178-li1x1m25 26 55 ] ] -RRB- cord-013178-li1x1m25 26 56 [ [ -LRB- cord-013178-li1x1m25 26 57 25 25 CD cord-013178-li1x1m25 26 58 ] ] -RRB- cord-013178-li1x1m25 26 59 [ [ -LRB- cord-013178-li1x1m25 26 60 26 26 CD cord-013178-li1x1m25 26 61 ] ] -RRB- cord-013178-li1x1m25 26 62 [ [ -LRB- cord-013178-li1x1m25 26 63 27 27 CD cord-013178-li1x1m25 26 64 ] ] -RRB- cord-013178-li1x1m25 26 65 . . . cord-013178-li1x1m25 27 1 The the DT cord-013178-li1x1m25 27 2 open open JJ cord-013178-li1x1m25 27 3 reading reading NN cord-013178-li1x1m25 27 4 frame frame NN cord-013178-li1x1m25 27 5 1 1 CD cord-013178-li1x1m25 27 6 ( ( -LRB- cord-013178-li1x1m25 27 7 ORF1 ORF1 NNP cord-013178-li1x1m25 27 8 ) ) -RRB- cord-013178-li1x1m25 27 9 , , , cord-013178-li1x1m25 27 10 open open JJ cord-013178-li1x1m25 27 11 reading reading NN cord-013178-li1x1m25 27 12 frame frame NN cord-013178-li1x1m25 27 13 2 2 CD cord-013178-li1x1m25 27 14 ( ( -LRB- cord-013178-li1x1m25 27 15 ORF2 ORF2 NNP cord-013178-li1x1m25 27 16 ) ) -RRB- cord-013178-li1x1m25 27 17 , , , cord-013178-li1x1m25 27 18 and and CC cord-013178-li1x1m25 27 19 open open JJ cord-013178-li1x1m25 27 20 reading reading NN cord-013178-li1x1m25 27 21 frame frame NN cord-013178-li1x1m25 27 22 3 3 CD cord-013178-li1x1m25 28 1 ( ( -LRB- cord-013178-li1x1m25 28 2 ORF3 ORF3 NNP cord-013178-li1x1m25 28 3 ) ) -RRB- cord-013178-li1x1m25 28 4 genes gene NNS cord-013178-li1x1m25 28 5 are be VBP cord-013178-li1x1m25 28 6 the the DT cord-013178-li1x1m25 28 7 three three CD cord-013178-li1x1m25 28 8 principal principal NN cord-013178-li1x1m25 28 9 ORFs orfs NN cord-013178-li1x1m25 28 10 among among IN cord-013178-li1x1m25 28 11 11 11 CD cord-013178-li1x1m25 28 12 ORFs orf NNS cord-013178-li1x1m25 28 13 in in IN cord-013178-li1x1m25 28 14 PCVs pcv NNS cord-013178-li1x1m25 28 15 [ [ -LRB- cord-013178-li1x1m25 28 16 28 28 CD cord-013178-li1x1m25 28 17 ] ] -RRB- cord-013178-li1x1m25 28 18 . . . cord-013178-li1x1m25 29 1 The the DT cord-013178-li1x1m25 29 2 ORF1 ORF1 NNP cord-013178-li1x1m25 29 3 encodes encode VBZ cord-013178-li1x1m25 29 4 for for IN cord-013178-li1x1m25 29 5 the the DT cord-013178-li1x1m25 29 6 replicase replicase NN cord-013178-li1x1m25 29 7 Peptide Peptide NNP cord-013178-li1x1m25 29 8 C3 C3 NNP cord-013178-li1x1m25 29 9 and and CC cord-013178-li1x1m25 29 10 peptide peptide JJ cord-013178-li1x1m25 29 11 N1 n1 NN cord-013178-li1x1m25 29 12 were be VBD cord-013178-li1x1m25 29 13 appended append VBN cord-013178-li1x1m25 29 14 with with IN cord-013178-li1x1m25 29 15 an an DT cord-013178-li1x1m25 29 16 N n CD cord-013178-li1x1m25 29 17 - - HYPH cord-013178-li1x1m25 29 18 terminal terminal NN cord-013178-li1x1m25 29 19 cysteine cysteine NN cord-013178-li1x1m25 29 20 during during IN cord-013178-li1x1m25 29 21 synthesis synthesis NN cord-013178-li1x1m25 29 22 , , , cord-013178-li1x1m25 29 23 which which WDT cord-013178-li1x1m25 29 24 was be VBD cord-013178-li1x1m25 29 25 required require VBN cord-013178-li1x1m25 29 26 for for IN cord-013178-li1x1m25 29 27 conjugation conjugation NN cord-013178-li1x1m25 29 28 with with IN cord-013178-li1x1m25 29 29 maleimide maleimide NN cord-013178-li1x1m25 29 30 - - HYPH cord-013178-li1x1m25 29 31 activated activate VBN cord-013178-li1x1m25 29 32 carriers carrier NNS cord-013178-li1x1m25 29 33 . . . cord-013178-li1x1m25 30 1 Animal animal NN cord-013178-li1x1m25 30 2 experiments experiment NNS cord-013178-li1x1m25 30 3 in in IN cord-013178-li1x1m25 30 4 this this DT cord-013178-li1x1m25 30 5 study study NN cord-013178-li1x1m25 30 6 were be VBD cord-013178-li1x1m25 30 7 performed perform VBN cord-013178-li1x1m25 30 8 following follow VBG cord-013178-li1x1m25 30 9 the the DT cord-013178-li1x1m25 30 10 current current JJ cord-013178-li1x1m25 30 11 legislation legislation NN cord-013178-li1x1m25 30 12 on on IN cord-013178-li1x1m25 30 13 ethical ethical JJ cord-013178-li1x1m25 30 14 and and CC cord-013178-li1x1m25 30 15 welfare welfare NN cord-013178-li1x1m25 30 16 recommendations recommendation NNS cord-013178-li1x1m25 30 17 . . . cord-013178-li1x1m25 31 1 The the DT cord-013178-li1x1m25 31 2 experiments experiment NNS cord-013178-li1x1m25 31 3 followed follow VBD cord-013178-li1x1m25 31 4 the the DT cord-013178-li1x1m25 31 5 standards standard NNS cord-013178-li1x1m25 31 6 of of IN cord-013178-li1x1m25 31 7 the the DT cord-013178-li1x1m25 31 8 Guide Guide NNP cord-013178-li1x1m25 31 9 of of IN cord-013178-li1x1m25 31 10 the the DT cord-013178-li1x1m25 31 11 Care Care NNP cord-013178-li1x1m25 31 12 and and CC cord-013178-li1x1m25 31 13 Use Use NNP cord-013178-li1x1m25 31 14 of of IN cord-013178-li1x1m25 31 15 Laboratory Laboratory NNP cord-013178-li1x1m25 31 16 Animals Animals NNPS cord-013178-li1x1m25 31 17 . . . cord-013178-li1x1m25 32 1 All all DT cord-013178-li1x1m25 32 2 animal animal NN cord-013178-li1x1m25 32 3 work work NN cord-013178-li1x1m25 32 4 was be VBD cord-013178-li1x1m25 32 5 approved approve VBN cord-013178-li1x1m25 32 6 by by IN cord-013178-li1x1m25 32 7 the the DT cord-013178-li1x1m25 32 8 Institutional Institutional NNP cord-013178-li1x1m25 32 9 Animal Animal NNP cord-013178-li1x1m25 32 10 Care Care NNP cord-013178-li1x1m25 32 11 and and CC cord-013178-li1x1m25 32 12 Use Use NNP cord-013178-li1x1m25 32 13 Committee Committee NNP cord-013178-li1x1m25 32 14 ( ( -LRB- cord-013178-li1x1m25 32 15 IACUC IACUC NNP cord-013178-li1x1m25 32 16 ) ) -RRB- cord-013178-li1x1m25 32 17 of of IN cord-013178-li1x1m25 32 18 the the DT cord-013178-li1x1m25 32 19 Livestock Livestock NNP cord-013178-li1x1m25 32 20 Research Research NNP cord-013178-li1x1m25 32 21 Institute Institute NNP cord-013178-li1x1m25 32 22 , , , cord-013178-li1x1m25 32 23 and and CC cord-013178-li1x1m25 32 24 the the DT cord-013178-li1x1m25 32 25 IACUC IACUC NNP cord-013178-li1x1m25 32 26 of of IN cord-013178-li1x1m25 32 27 the the DT cord-013178-li1x1m25 32 28 Animal Animal NNP cord-013178-li1x1m25 32 29 Health Health NNP cord-013178-li1x1m25 32 30 Research Research NNP cord-013178-li1x1m25 32 31 Institute Institute NNP cord-013178-li1x1m25 32 32 . . . cord-013178-li1x1m25 33 1 The the DT cord-013178-li1x1m25 33 2 IACUC IACUC NNP cord-013178-li1x1m25 33 3 approval approval NN cord-013178-li1x1m25 33 4 numbers number NNS cord-013178-li1x1m25 33 5 LRIIACUC100 LRIIACUC100 NNP cord-013178-li1x1m25 33 6 - - HYPH cord-013178-li1x1m25 33 7 33 33 CD cord-013178-li1x1m25 34 1 ( ( -LRB- cord-013178-li1x1m25 34 2 for for IN cord-013178-li1x1m25 34 3 swine swine NN cord-013178-li1x1m25 34 4 and and CC cord-013178-li1x1m25 34 5 murine murine JJ cord-013178-li1x1m25 34 6 experiments experiment NNS cord-013178-li1x1m25 34 7 ) ) -RRB- cord-013178-li1x1m25 34 8 , , , cord-013178-li1x1m25 34 9 A00027 A00027 NNP cord-013178-li1x1m25 34 10 ( ( -LRB- cord-013178-li1x1m25 34 11 for for IN cord-013178-li1x1m25 34 12 murine murine JJ cord-013178-li1x1m25 34 13 and and CC cord-013178-li1x1m25 34 14 rabbit rabbit NN cord-013178-li1x1m25 34 15 experiments experiment NNS cord-013178-li1x1m25 34 16 ) ) -RRB- cord-013178-li1x1m25 34 17 , , , cord-013178-li1x1m25 34 18 and and CC cord-013178-li1x1m25 34 19 A02023 A02023 NNP cord-013178-li1x1m25 34 20 ( ( -LRB- cord-013178-li1x1m25 34 21 for for IN cord-013178-li1x1m25 34 22 murine murine JJ cord-013178-li1x1m25 34 23 and and CC cord-013178-li1x1m25 34 24 rabbit rabbit NN cord-013178-li1x1m25 34 25 experiments experiment NNS cord-013178-li1x1m25 34 26 ) ) -RRB- cord-013178-li1x1m25 34 27 were be VBD cord-013178-li1x1m25 34 28 given give VBN cord-013178-li1x1m25 34 29 in in IN cord-013178-li1x1m25 34 30 this this DT cord-013178-li1x1m25 34 31 study study NN cord-013178-li1x1m25 34 32 . . . cord-013178-li1x1m25 35 1 Antisera Antisera NNP cord-013178-li1x1m25 35 2 against against IN cord-013178-li1x1m25 35 3 PCV2 PCV2 NNPS cord-013178-li1x1m25 35 4 were be VBD cord-013178-li1x1m25 35 5 generated generate VBN cord-013178-li1x1m25 35 6 by by IN cord-013178-li1x1m25 35 7 immunizing immunize VBG cord-013178-li1x1m25 35 8 New New NNP cord-013178-li1x1m25 35 9 Zealand Zealand NNP cord-013178-li1x1m25 35 10 white white JJ cord-013178-li1x1m25 35 11 rabbits rabbit NNS cord-013178-li1x1m25 35 12 ( ( -LRB- cord-013178-li1x1m25 35 13 from from IN cord-013178-li1x1m25 35 14 Livestock Livestock NNP cord-013178-li1x1m25 35 15 Research Research NNP cord-013178-li1x1m25 35 16 Institute Institute NNP cord-013178-li1x1m25 35 17 , , , cord-013178-li1x1m25 35 18 Council Council NNP cord-013178-li1x1m25 35 19 of of IN cord-013178-li1x1m25 35 20 Agriculture Agriculture NNP cord-013178-li1x1m25 35 21 , , , cord-013178-li1x1m25 35 22 Taiwan Taiwan NNP cord-013178-li1x1m25 35 23 ) ) -RRB- cord-013178-li1x1m25 35 24 . . . cord-013178-li1x1m25 36 1 Rabbits rabbit NNS cord-013178-li1x1m25 36 2 were be VBD cord-013178-li1x1m25 36 3 maintained maintain VBN cord-013178-li1x1m25 36 4 in in IN cord-013178-li1x1m25 36 5 isolation isolation NN cord-013178-li1x1m25 36 6 rooms room NNS cord-013178-li1x1m25 36 7 , , , cord-013178-li1x1m25 36 8 and and CC cord-013178-li1x1m25 36 9 the the DT cord-013178-li1x1m25 36 10 room room NN cord-013178-li1x1m25 36 11 temperature temperature NN cord-013178-li1x1m25 36 12 was be VBD cord-013178-li1x1m25 36 13 at at IN cord-013178-li1x1m25 36 14 20 20 CD cord-013178-li1x1m25 36 15 - - SYM cord-013178-li1x1m25 36 16 26 26 CD cord-013178-li1x1m25 37 1 • • NNP cord-013178-li1x1m25 38 1 C. C. NNP cord-013178-li1x1m25 38 2 They -PRON- PRP cord-013178-li1x1m25 38 3 were be VBD cord-013178-li1x1m25 38 4 fed feed VBN cord-013178-li1x1m25 38 5 with with IN cord-013178-li1x1m25 38 6 a a DT cord-013178-li1x1m25 38 7 commercially commercially RB cord-013178-li1x1m25 38 8 pelleted pellete VBN cord-013178-li1x1m25 38 9 diet diet NN cord-013178-li1x1m25 38 10 ( ( -LRB- cord-013178-li1x1m25 38 11 Fwusow Fwusow NNP cord-013178-li1x1m25 38 12 In In NNP cord-013178-li1x1m25 38 13 . . . cord-013178-li1x1m25 38 14 , , , cord-013178-li1x1m25 38 15 Taiwan Taiwan NNP cord-013178-li1x1m25 38 16 ) ) -RRB- cord-013178-li1x1m25 38 17 , , , cord-013178-li1x1m25 38 18 and and CC cord-013178-li1x1m25 38 19 pure pure JJ cord-013178-li1x1m25 38 20 water water NN cord-013178-li1x1m25 38 21 was be VBD cord-013178-li1x1m25 38 22 available available JJ cord-013178-li1x1m25 38 23 ad ad NN cord-013178-li1x1m25 38 24 libitum libitum NN cord-013178-li1x1m25 38 25 . . . cord-013178-li1x1m25 39 1 Then then RB cord-013178-li1x1m25 39 2 , , , cord-013178-li1x1m25 39 3 animals animal NNS cord-013178-li1x1m25 39 4 were be VBD cord-013178-li1x1m25 39 5 immunized immunize VBN cord-013178-li1x1m25 39 6 with with IN cord-013178-li1x1m25 39 7 peptide peptide NN cord-013178-li1x1m25 39 8 immunogen immunogen NN cord-013178-li1x1m25 39 9 ( ( -LRB- cord-013178-li1x1m25 39 10 the the DT cord-013178-li1x1m25 39 11 peptide peptide NN cord-013178-li1x1m25 39 12 N1 n1 NN cord-013178-li1x1m25 39 13 conjugated conjugate VBN cord-013178-li1x1m25 39 14 with with IN cord-013178-li1x1m25 39 15 KLH KLH NNP cord-013178-li1x1m25 39 16 ) ) -RRB- cord-013178-li1x1m25 39 17 or or CC cord-013178-li1x1m25 39 18 commercial commercial JJ cord-013178-li1x1m25 39 19 vaccine vaccine NN cord-013178-li1x1m25 39 20 five five CD cord-013178-li1x1m25 39 21 times time NNS cord-013178-li1x1m25 39 22 at at IN cord-013178-li1x1m25 39 23 two two CD cord-013178-li1x1m25 39 24 - - HYPH cord-013178-li1x1m25 39 25 week week NN cord-013178-li1x1m25 39 26 intervals interval NNS cord-013178-li1x1m25 39 27 . . . cord-013178-li1x1m25 40 1 For for IN cord-013178-li1x1m25 40 2 peptide peptide JJ cord-013178-li1x1m25 40 3 immunization immunization NN cord-013178-li1x1m25 40 4 , , , cord-013178-li1x1m25 40 5 an an DT cord-013178-li1x1m25 40 6 initial initial JJ cord-013178-li1x1m25 40 7 dose dose NN cord-013178-li1x1m25 40 8 of of IN cord-013178-li1x1m25 40 9 150 150 CD cord-013178-li1x1m25 40 10 µg µg NNS cord-013178-li1x1m25 40 11 of of IN cord-013178-li1x1m25 40 12 the the DT cord-013178-li1x1m25 40 13 conjugated conjugated JJ cord-013178-li1x1m25 40 14 peptide peptide NN cord-013178-li1x1m25 40 15 was be VBD cord-013178-li1x1m25 40 16 mixed mix VBN cord-013178-li1x1m25 40 17 with with IN cord-013178-li1x1m25 40 18 complete complete JJ cord-013178-li1x1m25 40 19 Freund Freund NNP cord-013178-li1x1m25 40 20 's 's POS cord-013178-li1x1m25 40 21 adjuvant adjuvant NN cord-013178-li1x1m25 40 22 ( ( -LRB- cord-013178-li1x1m25 40 23 Sigma Sigma NNP cord-013178-li1x1m25 40 24 - - HYPH cord-013178-li1x1m25 40 25 Aldrich Aldrich NNP cord-013178-li1x1m25 40 26 , , , cord-013178-li1x1m25 40 27 St. St. NNP cord-013178-li1x1m25 40 28 Louis Louis NNP cord-013178-li1x1m25 40 29 , , , cord-013178-li1x1m25 40 30 MO MO NNP cord-013178-li1x1m25 40 31 , , , cord-013178-li1x1m25 40 32 USA USA NNP cord-013178-li1x1m25 40 33 ) ) -RRB- cord-013178-li1x1m25 40 34 at at IN cord-013178-li1x1m25 40 35 the the DT cord-013178-li1x1m25 40 36 first first JJ cord-013178-li1x1m25 40 37 injection injection NN cord-013178-li1x1m25 40 38 . . . cord-013178-li1x1m25 41 1 Each each DT cord-013178-li1x1m25 41 2 rabbit rabbit NN cord-013178-li1x1m25 41 3 was be VBD cord-013178-li1x1m25 41 4 re re VBN cord-013178-li1x1m25 41 5 - - VBN cord-013178-li1x1m25 41 6 immunized immunize VBN cord-013178-li1x1m25 41 7 with with IN cord-013178-li1x1m25 41 8 the the DT cord-013178-li1x1m25 41 9 same same JJ cord-013178-li1x1m25 41 10 amount amount NN cord-013178-li1x1m25 41 11 of of IN cord-013178-li1x1m25 41 12 immunogenic immunogenic JJ cord-013178-li1x1m25 41 13 mixture mixture NN cord-013178-li1x1m25 41 14 with with IN cord-013178-li1x1m25 41 15 incomplete incomplete JJ cord-013178-li1x1m25 41 16 Freund Freund NNP cord-013178-li1x1m25 41 17 s s POS cord-013178-li1x1m25 41 18 adjuvant adjuvant NN cord-013178-li1x1m25 41 19 ( ( -LRB- cord-013178-li1x1m25 41 20 Sigma Sigma NNP cord-013178-li1x1m25 41 21 - - HYPH cord-013178-li1x1m25 41 22 Aldrich Aldrich NNP cord-013178-li1x1m25 41 23 , , , cord-013178-li1x1m25 41 24 St. St. NNP cord-013178-li1x1m25 41 25 Louis Louis NNP cord-013178-li1x1m25 41 26 , , , cord-013178-li1x1m25 41 27 MO MO NNP cord-013178-li1x1m25 41 28 , , , cord-013178-li1x1m25 41 29 USA USA NNP cord-013178-li1x1m25 41 30 ) ) -RRB- cord-013178-li1x1m25 41 31 . . . cord-013178-li1x1m25 42 1 For for IN cord-013178-li1x1m25 42 2 virus virus NN cord-013178-li1x1m25 42 3 - - HYPH cord-013178-li1x1m25 42 4 like like JJ cord-013178-li1x1m25 42 5 particles particle NNS cord-013178-li1x1m25 42 6 ( ( -LRB- cord-013178-li1x1m25 42 7 VLP VLP NNP cord-013178-li1x1m25 42 8 ) ) -RRB- cord-013178-li1x1m25 42 9 of of IN cord-013178-li1x1m25 42 10 PCV2 pcv2 JJ cord-013178-li1x1m25 42 11 immunization immunization NN cord-013178-li1x1m25 42 12 , , , cord-013178-li1x1m25 42 13 the the DT cord-013178-li1x1m25 42 14 rabbit rabbit NN cord-013178-li1x1m25 42 15 was be VBD cord-013178-li1x1m25 42 16 injected inject VBN cord-013178-li1x1m25 42 17 intramuscularly intramuscularly RB cord-013178-li1x1m25 42 18 in in IN cord-013178-li1x1m25 42 19 the the DT cord-013178-li1x1m25 42 20 legs leg NNS cord-013178-li1x1m25 42 21 with with IN cord-013178-li1x1m25 42 22 0.5 0.5 CD cord-013178-li1x1m25 42 23 mL ml NN cord-013178-li1x1m25 42 24 of of IN cord-013178-li1x1m25 42 25 the the DT cord-013178-li1x1m25 42 26 PCV2 PCV2 NNP cord-013178-li1x1m25 42 27 vaccine vaccine NN cord-013178-li1x1m25 42 28 ( ( -LRB- cord-013178-li1x1m25 42 29 CircoFLEX circoflex NN cord-013178-li1x1m25 42 30 ® ® NNP cord-013178-li1x1m25 42 31 , , , cord-013178-li1x1m25 42 32 Boehringer Boehringer NNP cord-013178-li1x1m25 42 33 Ingelheim Ingelheim NNP cord-013178-li1x1m25 42 34 ) ) -RRB- cord-013178-li1x1m25 42 35 . . . cord-013178-li1x1m25 43 1 The the DT cord-013178-li1x1m25 43 2 blood blood NN cord-013178-li1x1m25 43 3 was be VBD cord-013178-li1x1m25 43 4 harvested harvest VBN cord-013178-li1x1m25 43 5 two two CD cord-013178-li1x1m25 43 6 weeks week NNS cord-013178-li1x1m25 43 7 after after IN cord-013178-li1x1m25 43 8 the the DT cord-013178-li1x1m25 43 9 last last JJ cord-013178-li1x1m25 43 10 injection injection NN cord-013178-li1x1m25 43 11 . . . cord-013178-li1x1m25 44 1 The the DT cord-013178-li1x1m25 44 2 antibody antibody NN cord-013178-li1x1m25 44 3 titers titer NNS cord-013178-li1x1m25 44 4 were be VBD cord-013178-li1x1m25 44 5 measured measure VBN cord-013178-li1x1m25 44 6 by by IN cord-013178-li1x1m25 44 7 indirect indirect JJ cord-013178-li1x1m25 44 8 enzyme enzyme NN cord-013178-li1x1m25 44 9 - - HYPH cord-013178-li1x1m25 44 10 linked link VBN cord-013178-li1x1m25 44 11 immunosorbent immunosorbent JJ cord-013178-li1x1m25 44 12 assay assay NN cord-013178-li1x1m25 44 13 ( ( -LRB- cord-013178-li1x1m25 44 14 iELISA iELISA NNP cord-013178-li1x1m25 44 15 ) ) -RRB- cord-013178-li1x1m25 44 16 [ [ -LRB- cord-013178-li1x1m25 44 17 48 48 CD cord-013178-li1x1m25 44 18 ] ] -RRB- cord-013178-li1x1m25 44 19 ( ( -LRB- cord-013178-li1x1m25 44 20 performed perform VBN cord-013178-li1x1m25 44 21 as as IN cord-013178-li1x1m25 44 22 described describe VBN cord-013178-li1x1m25 44 23 previously previously RB cord-013178-li1x1m25 44 24 for for IN cord-013178-li1x1m25 44 25 mouse mouse NN cord-013178-li1x1m25 44 26 anti anti JJ cord-013178-li1x1m25 44 27 - - JJ cord-013178-li1x1m25 44 28 PCV2 pcv2 JJ cord-013178-li1x1m25 44 29 ELISA ELISA NNP cord-013178-li1x1m25 44 30 , , , cord-013178-li1x1m25 44 31 with with IN cord-013178-li1x1m25 44 32 the the DT cord-013178-li1x1m25 44 33 exception exception NN cord-013178-li1x1m25 44 34 that that IN cord-013178-li1x1m25 44 35 peroxidase peroxidase NN cord-013178-li1x1m25 44 36 - - HYPH cord-013178-li1x1m25 44 37 conjugated conjugate VBN cord-013178-li1x1m25 44 38 donkey donkey NN cord-013178-li1x1m25 44 39 anti anti JJ cord-013178-li1x1m25 44 40 - - JJ cord-013178-li1x1m25 44 41 rabbit rabbit JJ cord-013178-li1x1m25 44 42 IgG igg NN cord-013178-li1x1m25 44 43 ( ( -LRB- cord-013178-li1x1m25 44 44 H H NNP cord-013178-li1x1m25 44 45 + + CC cord-013178-li1x1m25 44 46 L L NNP cord-013178-li1x1m25 44 47 , , , cord-013178-li1x1m25 44 48 Jackson Jackson NNP cord-013178-li1x1m25 44 49 ImmunoResearch ImmunoResearch NNP cord-013178-li1x1m25 44 50 , , , cord-013178-li1x1m25 44 51 West West NNP cord-013178-li1x1m25 44 52 Grove Grove NNP cord-013178-li1x1m25 44 53 , , , cord-013178-li1x1m25 44 54 PA PA NNP cord-013178-li1x1m25 44 55 , , , cord-013178-li1x1m25 44 56 USA USA NNP cord-013178-li1x1m25 44 57 ) ) -RRB- cord-013178-li1x1m25 44 58 secondary secondary JJ cord-013178-li1x1m25 44 59 antibody antibody NN cord-013178-li1x1m25 44 60 was be VBD cord-013178-li1x1m25 44 61 used use VBN cord-013178-li1x1m25 44 62 ) ) -RRB- cord-013178-li1x1m25 44 63 . . . cord-013178-li1x1m25 45 1 Hybridomas Hybridomas NNP cord-013178-li1x1m25 45 2 were be VBD cord-013178-li1x1m25 45 3 generated generate VBN cord-013178-li1x1m25 45 4 following follow VBG cord-013178-li1x1m25 45 5 established establish VBN cord-013178-li1x1m25 45 6 methods method NNS cord-013178-li1x1m25 45 7 , , , cord-013178-li1x1m25 45 8 as as IN cord-013178-li1x1m25 45 9 previously previously RB cord-013178-li1x1m25 45 10 described describe VBN cord-013178-li1x1m25 45 11 [ [ -LRB- cord-013178-li1x1m25 45 12 49 49 CD cord-013178-li1x1m25 45 13 ] ] -RRB- cord-013178-li1x1m25 45 14 . . . cord-013178-li1x1m25 46 1 Briefly briefly RB cord-013178-li1x1m25 46 2 , , , cord-013178-li1x1m25 46 3 four four CD cord-013178-li1x1m25 46 4 five five CD cord-013178-li1x1m25 46 5 - - HYPH cord-013178-li1x1m25 46 6 week week NN cord-013178-li1x1m25 46 7 - - HYPH cord-013178-li1x1m25 46 8 old old JJ cord-013178-li1x1m25 46 9 , , , cord-013178-li1x1m25 46 10 female female JJ cord-013178-li1x1m25 46 11 , , , cord-013178-li1x1m25 46 12 BALB/ BALB/ NNP cord-013178-li1x1m25 47 1 cByJNarl cbyjnarl CD cord-013178-li1x1m25 48 1 mice mouse NNS cord-013178-li1x1m25 48 2 were be VBD cord-013178-li1x1m25 48 3 purchased purchase VBN cord-013178-li1x1m25 48 4 from from IN cord-013178-li1x1m25 48 5 a a DT cord-013178-li1x1m25 48 6 specific specific JJ cord-013178-li1x1m25 48 7 pathogen pathogen NN cord-013178-li1x1m25 48 8 - - HYPH cord-013178-li1x1m25 48 9 free free JJ cord-013178-li1x1m25 48 10 ( ( -LRB- cord-013178-li1x1m25 48 11 SPF SPF NNP cord-013178-li1x1m25 48 12 ) ) -RRB- cord-013178-li1x1m25 48 13 colony colony NN cord-013178-li1x1m25 48 14 ( ( -LRB- cord-013178-li1x1m25 48 15 the the DT cord-013178-li1x1m25 48 16 National National NNP cord-013178-li1x1m25 48 17 Applied Applied NNP cord-013178-li1x1m25 48 18 Research Research NNP cord-013178-li1x1m25 48 19 Laboratories Laboratories NNPS cord-013178-li1x1m25 48 20 , , , cord-013178-li1x1m25 48 21 Taiwan Taiwan NNP cord-013178-li1x1m25 48 22 ) ) -RRB- cord-013178-li1x1m25 48 23 . . . cord-013178-li1x1m25 49 1 The the DT cord-013178-li1x1m25 49 2 mice mouse NNS cord-013178-li1x1m25 49 3 were be VBD cord-013178-li1x1m25 49 4 maintained maintain VBN cord-013178-li1x1m25 49 5 in in IN cord-013178-li1x1m25 49 6 isolation isolation NN cord-013178-li1x1m25 49 7 rooms room NNS cord-013178-li1x1m25 49 8 in in IN cord-013178-li1x1m25 49 9 filtertop filtertop NNP cord-013178-li1x1m25 49 10 cages cage NNS cord-013178-li1x1m25 49 11 , , , cord-013178-li1x1m25 49 12 and and CC cord-013178-li1x1m25 49 13 the the DT cord-013178-li1x1m25 49 14 room room NN cord-013178-li1x1m25 49 15 temperature temperature NN cord-013178-li1x1m25 49 16 was be VBD cord-013178-li1x1m25 49 17 at at IN cord-013178-li1x1m25 49 18 20 20 CD cord-013178-li1x1m25 49 19 - - SYM cord-013178-li1x1m25 49 20 26 26 CD cord-013178-li1x1m25 50 1 • • NNP cord-013178-li1x1m25 51 1 C. C. NNP cord-013178-li1x1m25 51 2 Mice Mice NNP cord-013178-li1x1m25 51 3 were be VBD cord-013178-li1x1m25 51 4 fed feed VBN cord-013178-li1x1m25 51 5 with with IN cord-013178-li1x1m25 51 6 a a DT cord-013178-li1x1m25 51 7 commercially commercially RB cord-013178-li1x1m25 51 8 pelleted pellete VBN cord-013178-li1x1m25 51 9 diet diet NN cord-013178-li1x1m25 51 10 ( ( -LRB- cord-013178-li1x1m25 51 11 LabDiet LabDiet NNP cord-013178-li1x1m25 51 12 , , , cord-013178-li1x1m25 51 13 5002 5002 CD cord-013178-li1x1m25 51 14 , , , cord-013178-li1x1m25 51 15 St. St. NNP cord-013178-li1x1m25 51 16 Louis Louis NNP cord-013178-li1x1m25 51 17 , , , cord-013178-li1x1m25 51 18 MO MO NNP cord-013178-li1x1m25 51 19 , , , cord-013178-li1x1m25 51 20 USA USA NNP cord-013178-li1x1m25 51 21 ) ) -RRB- cord-013178-li1x1m25 51 22 , , , cord-013178-li1x1m25 51 23 and and CC cord-013178-li1x1m25 51 24 pure pure JJ cord-013178-li1x1m25 51 25 water water NN cord-013178-li1x1m25 51 26 was be VBD cord-013178-li1x1m25 51 27 available available JJ cord-013178-li1x1m25 51 28 ad ad NN cord-013178-li1x1m25 51 29 libitum libitum NN cord-013178-li1x1m25 51 30 . . . cord-013178-li1x1m25 52 1 Mice mouse NNS cord-013178-li1x1m25 52 2 were be VBD cord-013178-li1x1m25 52 3 inoculated inoculate VBN cord-013178-li1x1m25 52 4 with with IN cord-013178-li1x1m25 52 5 65 65 CD cord-013178-li1x1m25 52 6 µg µg NN cord-013178-li1x1m25 52 7 of of IN cord-013178-li1x1m25 52 8 immunogen immunogen NN cord-013178-li1x1m25 52 9 ( ( -LRB- cord-013178-li1x1m25 52 10 the the DT cord-013178-li1x1m25 52 11 peptide peptide NN cord-013178-li1x1m25 52 12 N1 n1 NN cord-013178-li1x1m25 52 13 conjugated conjugate VBN cord-013178-li1x1m25 52 14 with with IN cord-013178-li1x1m25 52 15 KLH KLH NNP cord-013178-li1x1m25 52 16 ) ) -RRB- cord-013178-li1x1m25 52 17 emulsified emulsify VBN cord-013178-li1x1m25 52 18 in in IN cord-013178-li1x1m25 52 19 Freund Freund NNP cord-013178-li1x1m25 52 20 's 's POS cord-013178-li1x1m25 52 21 adjuvant adjuvant NN cord-013178-li1x1m25 52 22 . . . cord-013178-li1x1m25 53 1 Subsequently subsequently RB cord-013178-li1x1m25 53 2 , , , cord-013178-li1x1m25 53 3 mice mouse NNS cord-013178-li1x1m25 53 4 were be VBD cord-013178-li1x1m25 53 5 boosted boost VBN cord-013178-li1x1m25 53 6 fortnightly fortnightly RB cord-013178-li1x1m25 53 7 with with IN cord-013178-li1x1m25 53 8 the the DT cord-013178-li1x1m25 53 9 same same JJ cord-013178-li1x1m25 53 10 amount amount NN cord-013178-li1x1m25 53 11 of of IN cord-013178-li1x1m25 53 12 immunogen immunogen NN cord-013178-li1x1m25 53 13 . . . cord-013178-li1x1m25 54 1 Three three CD cord-013178-li1x1m25 54 2 days day NNS cord-013178-li1x1m25 54 3 after after IN cord-013178-li1x1m25 54 4 the the DT cord-013178-li1x1m25 54 5 final final JJ cord-013178-li1x1m25 54 6 booster booster NN cord-013178-li1x1m25 54 7 , , , cord-013178-li1x1m25 54 8 the the DT cord-013178-li1x1m25 54 9 spleens spleen NNS cord-013178-li1x1m25 54 10 of of IN cord-013178-li1x1m25 54 11 the the DT cord-013178-li1x1m25 54 12 mice mouse NNS cord-013178-li1x1m25 54 13 were be VBD cord-013178-li1x1m25 54 14 harvested harvest VBN cord-013178-li1x1m25 54 15 for for IN cord-013178-li1x1m25 54 16 hybridoma hybridoma NN cord-013178-li1x1m25 54 17 generation generation NN cord-013178-li1x1m25 54 18 . . . cord-013178-li1x1m25 55 1 Hybridomas Hybridomas NNP cord-013178-li1x1m25 55 2 were be VBD cord-013178-li1x1m25 55 3 screened screen VBN cord-013178-li1x1m25 55 4 for for IN cord-013178-li1x1m25 55 5 the the DT cord-013178-li1x1m25 55 6 secretion secretion NN cord-013178-li1x1m25 55 7 of of IN cord-013178-li1x1m25 55 8 anti anti JJ cord-013178-li1x1m25 55 9 - - JJ cord-013178-li1x1m25 55 10 ORF3-specific orf3-specific JJ cord-013178-li1x1m25 55 11 mAbs mAbs NNP cord-013178-li1x1m25 55 12 by by IN cord-013178-li1x1m25 55 13 iELISA iELISA NNP cord-013178-li1x1m25 55 14 [ [ -LRB- cord-013178-li1x1m25 55 15 49 49 CD cord-013178-li1x1m25 55 16 ] ] -RRB- cord-013178-li1x1m25 55 17 using use VBG cord-013178-li1x1m25 55 18 the the DT cord-013178-li1x1m25 55 19 peptide peptide NN cord-013178-li1x1m25 55 20 N1 n1 NN cord-013178-li1x1m25 55 21 as as IN cord-013178-li1x1m25 55 22 a a DT cord-013178-li1x1m25 55 23 coating coating NN cord-013178-li1x1m25 55 24 antigen antigen NN cord-013178-li1x1m25 55 25 . . . cord-013178-li1x1m25 56 1 That that DT cord-013178-li1x1m25 56 2 secondary secondary JJ cord-013178-li1x1m25 56 3 antibody antibody NN cord-013178-li1x1m25 56 4 solution solution NN cord-013178-li1x1m25 56 5 ( ( -LRB- cord-013178-li1x1m25 56 6 HRP HRP NNP cord-013178-li1x1m25 56 7 - - HYPH cord-013178-li1x1m25 56 8 conjugated conjugate VBN cord-013178-li1x1m25 56 9 goat goat NN cord-013178-li1x1m25 56 10 anti anti JJ cord-013178-li1x1m25 56 11 - - JJ cord-013178-li1x1m25 56 12 mouse mouse NNP cord-013178-li1x1m25 56 13 IgA IgA NNP cord-013178-li1x1m25 56 14 / / SYM cord-013178-li1x1m25 56 15 IgG IgG NNP cord-013178-li1x1m25 56 16 / / SYM cord-013178-li1x1m25 56 17 IgM IgM NNP cord-013178-li1x1m25 56 18 , , , cord-013178-li1x1m25 57 1 H H NNP cord-013178-li1x1m25 57 2 / / SYM cord-013178-li1x1m25 57 3 L L NNP cord-013178-li1x1m25 57 4 chain chain NN cord-013178-li1x1m25 57 5 ( ( -LRB- cord-013178-li1x1m25 57 6 Novus Novus NNP cord-013178-li1x1m25 57 7 , , , cord-013178-li1x1m25 57 8 Saint Saint NNP cord-013178-li1x1m25 57 9 Charles Charles NNP cord-013178-li1x1m25 57 10 , , , cord-013178-li1x1m25 57 11 MO MO NNP cord-013178-li1x1m25 57 12 , , , cord-013178-li1x1m25 57 13 USA USA NNP cord-013178-li1x1m25 57 14 ) ) -RRB- cord-013178-li1x1m25 57 15 was be VBD cord-013178-li1x1m25 57 16 used use VBN cord-013178-li1x1m25 57 17 at at IN cord-013178-li1x1m25 57 18 1:2500 1:2500 CD cord-013178-li1x1m25 57 19 dilution dilution NN cord-013178-li1x1m25 57 20 for for IN cord-013178-li1x1m25 57 21 hybridoma hybridoma NN cord-013178-li1x1m25 57 22 screening screening NN cord-013178-li1x1m25 57 23 . . . cord-013178-li1x1m25 58 1 The the DT cord-013178-li1x1m25 58 2 hybridomas hybridomas NN cord-013178-li1x1m25 58 3 that that WDT cord-013178-li1x1m25 58 4 secreted secrete VBD cord-013178-li1x1m25 58 5 anti anti NNP cord-013178-li1x1m25 58 6 - - NNP cord-013178-li1x1m25 58 7 N1 N1 NNP cord-013178-li1x1m25 58 8 mAbs mAbs NNP cord-013178-li1x1m25 58 9 were be VBD cord-013178-li1x1m25 58 10 subcloned subclone VBN cord-013178-li1x1m25 58 11 by by IN cord-013178-li1x1m25 58 12 limited limited JJ cord-013178-li1x1m25 58 13 dilution dilution NN cord-013178-li1x1m25 58 14 of of IN cord-013178-li1x1m25 58 15 the the DT cord-013178-li1x1m25 58 16 cells cell NNS cord-013178-li1x1m25 58 17 . . . cord-013178-li1x1m25 59 1 Then then RB cord-013178-li1x1m25 59 2 , , , cord-013178-li1x1m25 59 3 ascites ascites NN cord-013178-li1x1m25 59 4 containing contain VBG cord-013178-li1x1m25 59 5 mAbs mAbs NNP cord-013178-li1x1m25 59 6 were be VBD cord-013178-li1x1m25 59 7 collected collect VBN cord-013178-li1x1m25 59 8 as as IN cord-013178-li1x1m25 59 9 previously previously RB cord-013178-li1x1m25 59 10 described describe VBN cord-013178-li1x1m25 59 11 [ [ -LRB- cord-013178-li1x1m25 59 12 49 49 CD cord-013178-li1x1m25 59 13 ] ] -RRB- cord-013178-li1x1m25 59 14 . . . cord-013178-li1x1m25 60 1 Subsequently subsequently RB cord-013178-li1x1m25 60 2 , , , cord-013178-li1x1m25 60 3 the the DT cord-013178-li1x1m25 60 4 mAbs mabs NN cord-013178-li1x1m25 60 5 were be VBD cord-013178-li1x1m25 60 6 purified purify VBN cord-013178-li1x1m25 60 7 from from IN cord-013178-li1x1m25 60 8 the the DT cord-013178-li1x1m25 60 9 ascites ascites NN cord-013178-li1x1m25 60 10 by by IN cord-013178-li1x1m25 60 11 using use VBG cord-013178-li1x1m25 60 12 the the DT cord-013178-li1x1m25 60 13 NAb NAb NNP cord-013178-li1x1m25 60 14 Protein Protein NNP cord-013178-li1x1m25 60 15 G G NNP cord-013178-li1x1m25 60 16 Spin Spin NNP cord-013178-li1x1m25 60 17 Column column NN cord-013178-li1x1m25 60 18 ( ( -LRB- cord-013178-li1x1m25 60 19 Thermo Thermo NNP cord-013178-li1x1m25 60 20 Scientific Scientific NNP cord-013178-li1x1m25 60 21 , , , cord-013178-li1x1m25 60 22 Rockford Rockford NNP cord-013178-li1x1m25 60 23 , , , cord-013178-li1x1m25 60 24 IL IL NNP cord-013178-li1x1m25 60 25 , , , cord-013178-li1x1m25 60 26 USA USA NNP cord-013178-li1x1m25 60 27 ) ) -RRB- cord-013178-li1x1m25 60 28 , , , cord-013178-li1x1m25 60 29 then then RB cord-013178-li1x1m25 60 30 collecting collect VBG cord-013178-li1x1m25 60 31 solutions solution NNS cord-013178-li1x1m25 60 32 were be VBD cord-013178-li1x1m25 60 33 exchanged exchange VBN cord-013178-li1x1m25 60 34 for for IN cord-013178-li1x1m25 60 35 the the DT cord-013178-li1x1m25 60 36 buffer buffer NN cord-013178-li1x1m25 60 37 and and CC cord-013178-li1x1m25 60 38 concentrated concentrate VBD cord-013178-li1x1m25 60 39 using use VBG cord-013178-li1x1m25 60 40 the the DT cord-013178-li1x1m25 60 41 Amicon Amicon NNP cord-013178-li1x1m25 60 42 Ultra Ultra NNP cord-013178-li1x1m25 60 43 50 50 CD cord-013178-li1x1m25 60 44 K K NNP cord-013178-li1x1m25 60 45 ( ( -LRB- cord-013178-li1x1m25 60 46 Millipore Millipore NNP cord-013178-li1x1m25 60 47 , , , cord-013178-li1x1m25 60 48 Burlington Burlington NNP cord-013178-li1x1m25 60 49 , , , cord-013178-li1x1m25 60 50 MA MA NNP cord-013178-li1x1m25 60 51 , , , cord-013178-li1x1m25 60 52 USA USA NNP cord-013178-li1x1m25 60 53 ) ) -RRB- cord-013178-li1x1m25 60 54 . . . cord-013178-li1x1m25 61 1 The the DT cord-013178-li1x1m25 61 2 concentration concentration NN cord-013178-li1x1m25 61 3 of of IN cord-013178-li1x1m25 61 4 mAbs mAbs NNP cord-013178-li1x1m25 61 5 was be VBD cord-013178-li1x1m25 61 6 determined determine VBN cord-013178-li1x1m25 61 7 using use VBG cord-013178-li1x1m25 61 8 the the DT cord-013178-li1x1m25 61 9 Nano Nano NNP cord-013178-li1x1m25 61 10 Photometer Photometer NNP cord-013178-li1x1m25 62 1 ® ® LS cord-013178-li1x1m25 62 2 ( ( -LRB- cord-013178-li1x1m25 62 3 Implen Implen NNP cord-013178-li1x1m25 62 4 , , , cord-013178-li1x1m25 62 5 Munich Munich NNP cord-013178-li1x1m25 62 6 , , , cord-013178-li1x1m25 62 7 Germany Germany NNP cord-013178-li1x1m25 62 8 ) ) -RRB- cord-013178-li1x1m25 62 9 . . . cord-013178-li1x1m25 63 1 The the DT cord-013178-li1x1m25 63 2 heavy heavy JJ cord-013178-li1x1m25 63 3 chain chain NN cord-013178-li1x1m25 63 4 and and CC cord-013178-li1x1m25 63 5 the the DT cord-013178-li1x1m25 63 6 light light JJ cord-013178-li1x1m25 63 7 chain chain NN cord-013178-li1x1m25 63 8 of of IN cord-013178-li1x1m25 63 9 the the DT cord-013178-li1x1m25 63 10 two two CD cord-013178-li1x1m25 63 11 mAbs mabs NN cord-013178-li1x1m25 63 12 secreted secrete VBN cord-013178-li1x1m25 63 13 by by IN cord-013178-li1x1m25 63 14 each each DT cord-013178-li1x1m25 63 15 hybridoma hybridoma NN cord-013178-li1x1m25 63 16 were be VBD cord-013178-li1x1m25 63 17 determined determine VBN cord-013178-li1x1m25 63 18 by by IN cord-013178-li1x1m25 63 19 using use VBG cord-013178-li1x1m25 63 20 an an DT cord-013178-li1x1m25 63 21 SBA SBA NNP cord-013178-li1x1m25 63 22 Clonotyping Clonotyping NNP cord-013178-li1x1m25 63 23 System System NNP cord-013178-li1x1m25 63 24 / / SYM cord-013178-li1x1m25 63 25 HRP HRP NNP cord-013178-li1x1m25 63 26 ( ( -LRB- cord-013178-li1x1m25 63 27 Southern Southern NNP cord-013178-li1x1m25 63 28 Biotechnology Biotechnology NNP cord-013178-li1x1m25 63 29 , , , cord-013178-li1x1m25 63 30 Birmingham Birmingham NNP cord-013178-li1x1m25 63 31 , , , cord-013178-li1x1m25 63 32 AL AL NNP cord-013178-li1x1m25 63 33 , , , cord-013178-li1x1m25 63 34 USA USA NNP cord-013178-li1x1m25 63 35 ) ) -RRB- cord-013178-li1x1m25 63 36 . . . cord-013178-li1x1m25 64 1 It -PRON- PRP cord-013178-li1x1m25 64 2 was be VBD cord-013178-li1x1m25 64 3 performed perform VBN cord-013178-li1x1m25 64 4 according accord VBG cord-013178-li1x1m25 64 5 to to IN cord-013178-li1x1m25 64 6 the the DT cord-013178-li1x1m25 64 7 manufacturer manufacturer NN cord-013178-li1x1m25 64 8 's 's POS cord-013178-li1x1m25 64 9 instructions instruction NNS cord-013178-li1x1m25 64 10 and and CC cord-013178-li1x1m25 64 11 a a DT cord-013178-li1x1m25 64 12 previously previously RB cord-013178-li1x1m25 64 13 published publish VBN cord-013178-li1x1m25 64 14 method method NN cord-013178-li1x1m25 64 15 [ [ -LRB- cord-013178-li1x1m25 64 16 49 49 CD cord-013178-li1x1m25 64 17 ] ] -RRB- cord-013178-li1x1m25 64 18 . . . cord-013178-li1x1m25 65 1 Briefly briefly RB cord-013178-li1x1m25 65 2 , , , cord-013178-li1x1m25 65 3 the the DT cord-013178-li1x1m25 65 4 Nunc Nunc NNP cord-013178-li1x1m25 65 5 MaxiSorp MaxiSorp NNP cord-013178-li1x1m25 65 6 flat flat JJ cord-013178-li1x1m25 65 7 - - HYPH cord-013178-li1x1m25 65 8 bottom bottom JJ cord-013178-li1x1m25 65 9 96-well 96-well CD cord-013178-li1x1m25 65 10 plate plate NN cord-013178-li1x1m25 65 11 ( ( -LRB- cord-013178-li1x1m25 65 12 Thermo Thermo NNP cord-013178-li1x1m25 65 13 Fisher Fisher NNP cord-013178-li1x1m25 65 14 Scientific Scientific NNP cord-013178-li1x1m25 65 15 , , , cord-013178-li1x1m25 65 16 Inc. Inc. NNP cord-013178-li1x1m25 65 17 , , , cord-013178-li1x1m25 65 18 Waltham Waltham NNP cord-013178-li1x1m25 65 19 , , , cord-013178-li1x1m25 65 20 MA MA NNP cord-013178-li1x1m25 65 21 , , , cord-013178-li1x1m25 65 22 USA USA NNP cord-013178-li1x1m25 65 23 ) ) -RRB- cord-013178-li1x1m25 65 24 was be VBD cord-013178-li1x1m25 65 25 coated coat VBN cord-013178-li1x1m25 65 26 with with IN cord-013178-li1x1m25 65 27 the the DT cord-013178-li1x1m25 65 28 capture capture NN cord-013178-li1x1m25 65 29 antibody antibody NN cord-013178-li1x1m25 65 30 ( ( -LRB- cord-013178-li1x1m25 65 31 goat goat NNP cord-013178-li1x1m25 65 32 anti anti JJ cord-013178-li1x1m25 65 33 - - JJ cord-013178-li1x1m25 65 34 mouse mouse NN cord-013178-li1x1m25 65 35 Ig Ig NNP cord-013178-li1x1m25 65 36 , , , cord-013178-li1x1m25 65 37 5 5 CD cord-013178-li1x1m25 65 38 µg µg NN cord-013178-li1x1m25 65 39 / / SYM cord-013178-li1x1m25 65 40 mL ml NN cord-013178-li1x1m25 65 41 ) ) -RRB- cord-013178-li1x1m25 65 42 in in IN cord-013178-li1x1m25 65 43 0.05-M 0.05-m CD cord-013178-li1x1m25 65 44 bicarbonate bicarbonate NN cord-013178-li1x1m25 65 45 buffer buffer NN cord-013178-li1x1m25 65 46 ( ( -LRB- cord-013178-li1x1m25 65 47 Sigma Sigma NNP cord-013178-li1x1m25 65 48 - - HYPH cord-013178-li1x1m25 65 49 Aldrich Aldrich NNP cord-013178-li1x1m25 65 50 , , , cord-013178-li1x1m25 65 51 St. St. NNP cord-013178-li1x1m25 65 52 Louis Louis NNP cord-013178-li1x1m25 65 53 , , , cord-013178-li1x1m25 65 54 MO MO NNP cord-013178-li1x1m25 65 55 , , , cord-013178-li1x1m25 65 56 USA USA NNP cord-013178-li1x1m25 65 57 ) ) -RRB- cord-013178-li1x1m25 65 58 at at IN cord-013178-li1x1m25 65 59 4 4 CD cord-013178-li1x1m25 65 60 • • NN cord-013178-li1x1m25 65 61 C C NNP cord-013178-li1x1m25 65 62 overnight overnight RB cord-013178-li1x1m25 65 63 . . . cord-013178-li1x1m25 66 1 After after IN cord-013178-li1x1m25 66 2 three three CD cord-013178-li1x1m25 66 3 washes wash NNS cord-013178-li1x1m25 66 4 with with IN cord-013178-li1x1m25 66 5 PBS PBS NNP cord-013178-li1x1m25 66 6 containing contain VBG cord-013178-li1x1m25 66 7 0.05 0.05 CD cord-013178-li1x1m25 66 8 % % NN cord-013178-li1x1m25 66 9 Tween Tween NNP cord-013178-li1x1m25 66 10 20 20 CD cord-013178-li1x1m25 66 11 ( ( -LRB- cord-013178-li1x1m25 66 12 PBST PBST NNP cord-013178-li1x1m25 66 13 ) ) -RRB- cord-013178-li1x1m25 66 14 , , , cord-013178-li1x1m25 66 15 the the DT cord-013178-li1x1m25 66 16 plate plate NN cord-013178-li1x1m25 66 17 was be VBD cord-013178-li1x1m25 66 18 blocked block VBN cord-013178-li1x1m25 66 19 with with IN cord-013178-li1x1m25 66 20 the the DT cord-013178-li1x1m25 66 21 blocking block VBG cord-013178-li1x1m25 66 22 buffer buffer NN cord-013178-li1x1m25 66 23 ( ( -LRB- cord-013178-li1x1m25 66 24 PBST PBST NNP cord-013178-li1x1m25 66 25 containing contain VBG cord-013178-li1x1m25 66 26 5 5 CD cord-013178-li1x1m25 66 27 % % NN cord-013178-li1x1m25 66 28 casein casein JJ cord-013178-li1x1m25 66 29 hydrolysate hydrolysate NN cord-013178-li1x1m25 66 30 ) ) -RRB- cord-013178-li1x1m25 66 31 at at IN cord-013178-li1x1m25 66 32 37 37 CD cord-013178-li1x1m25 67 1 • • NNP cord-013178-li1x1m25 67 2 C C NNP cord-013178-li1x1m25 67 3 for for IN cord-013178-li1x1m25 67 4 30 30 CD cord-013178-li1x1m25 67 5 min min NN cord-013178-li1x1m25 67 6 . . . cord-013178-li1x1m25 68 1 After after IN cord-013178-li1x1m25 68 2 washing washing NN cord-013178-li1x1m25 68 3 , , , cord-013178-li1x1m25 68 4 100 100 CD cord-013178-li1x1m25 68 5 µL µl CD cord-013178-li1x1m25 68 6 of of IN cord-013178-li1x1m25 68 7 each each DT cord-013178-li1x1m25 68 8 culture culture NN cord-013178-li1x1m25 68 9 supernatant supernatant VBN cord-013178-li1x1m25 68 10 of of IN cord-013178-li1x1m25 68 11 hybridoma hybridoma NN cord-013178-li1x1m25 68 12 were be VBD cord-013178-li1x1m25 68 13 added add VBN cord-013178-li1x1m25 68 14 sequentially sequentially RB cord-013178-li1x1m25 68 15 , , , cord-013178-li1x1m25 68 16 and and CC cord-013178-li1x1m25 68 17 the the DT cord-013178-li1x1m25 68 18 plate plate NN cord-013178-li1x1m25 68 19 was be VBD cord-013178-li1x1m25 68 20 incubated incubate VBN cord-013178-li1x1m25 68 21 at at IN cord-013178-li1x1m25 68 22 37 37 CD cord-013178-li1x1m25 68 23 • • NN cord-013178-li1x1m25 68 24 C c NN cord-013178-li1x1m25 68 25 for for IN cord-013178-li1x1m25 68 26 2 2 CD cord-013178-li1x1m25 68 27 h. h. NN cord-013178-li1x1m25 69 1 After after IN cord-013178-li1x1m25 69 2 washing washing NN cord-013178-li1x1m25 69 3 , , , cord-013178-li1x1m25 69 4 100 100 CD cord-013178-li1x1m25 69 5 µL µl CD cord-013178-li1x1m25 69 6 of of IN cord-013178-li1x1m25 69 7 HRP HRP NNP cord-013178-li1x1m25 69 8 labeled label VBN cord-013178-li1x1m25 69 9 goat goat NN cord-013178-li1x1m25 69 10 anti anti JJ cord-013178-li1x1m25 69 11 - - JJ cord-013178-li1x1m25 69 12 mouse mouse JJ cord-013178-li1x1m25 69 13 IgG1 igg1 NN cord-013178-li1x1m25 69 14 , , , cord-013178-li1x1m25 69 15 -IgG2a -igg2a JJ cord-013178-li1x1m25 69 16 , , , cord-013178-li1x1m25 69 17 -IgG2b -igg2b CC cord-013178-li1x1m25 69 18 , , , cord-013178-li1x1m25 69 19 -IgG3 -IgG3 NNP cord-013178-li1x1m25 69 20 , , , cord-013178-li1x1m25 69 21 -IgA -iga CD cord-013178-li1x1m25 69 22 , , , cord-013178-li1x1m25 69 23 -IgM -igm ADD cord-013178-li1x1m25 69 24 , , , cord-013178-li1x1m25 69 25 -κ -κ JJS cord-013178-li1x1m25 69 26 light light NN cord-013178-li1x1m25 69 27 chain chain NN cord-013178-li1x1m25 69 28 , , , cord-013178-li1x1m25 69 29 and and CC cord-013178-li1x1m25 69 30 -λ -λ WDT cord-013178-li1x1m25 69 31 light light JJ cord-013178-li1x1m25 69 32 chain chain NN cord-013178-li1x1m25 69 33 were be VBD cord-013178-li1x1m25 69 34 added add VBN cord-013178-li1x1m25 69 35 to to IN cord-013178-li1x1m25 69 36 appropriate appropriate JJ cord-013178-li1x1m25 69 37 wells well NNS cord-013178-li1x1m25 69 38 of of IN cord-013178-li1x1m25 69 39 the the DT cord-013178-li1x1m25 69 40 plate plate NN cord-013178-li1x1m25 69 41 . . . cord-013178-li1x1m25 70 1 PBST PBST NNP cord-013178-li1x1m25 70 2 served serve VBD cord-013178-li1x1m25 70 3 as as IN cord-013178-li1x1m25 70 4 background background NN cord-013178-li1x1m25 70 5 . . . cord-013178-li1x1m25 71 1 The the DT cord-013178-li1x1m25 71 2 plate plate NN cord-013178-li1x1m25 71 3 was be VBD cord-013178-li1x1m25 71 4 then then RB cord-013178-li1x1m25 71 5 incubated incubate VBN cord-013178-li1x1m25 71 6 at at IN cord-013178-li1x1m25 71 7 37 37 CD cord-013178-li1x1m25 71 8 • • NN cord-013178-li1x1m25 71 9 C c NN cord-013178-li1x1m25 71 10 for for IN cord-013178-li1x1m25 71 11 1 1 CD cord-013178-li1x1m25 71 12 h h NN cord-013178-li1x1m25 71 13 and and CC cord-013178-li1x1m25 71 14 after after IN cord-013178-li1x1m25 71 15 that that DT cord-013178-li1x1m25 71 16 washed wash VBD cord-013178-li1x1m25 71 17 with with IN cord-013178-li1x1m25 71 18 PBST PBST NNP cord-013178-li1x1m25 71 19 . . . cord-013178-li1x1m25 72 1 Finally finally RB cord-013178-li1x1m25 72 2 , , , cord-013178-li1x1m25 72 3 100 100 CD cord-013178-li1x1m25 72 4 µL µl CD cord-013178-li1x1m25 72 5 of of IN cord-013178-li1x1m25 72 6 2 2 CD cord-013178-li1x1m25 72 7 , , , cord-013178-li1x1m25 72 8 2 2 CD cord-013178-li1x1m25 72 9 -azino -azino SYM cord-013178-li1x1m25 72 10 - - HYPH cord-013178-li1x1m25 72 11 bis bis NN cord-013178-li1x1m25 72 12 ( ( -LRB- cord-013178-li1x1m25 72 13 3-ethylbenzothiazoline-6-sulfonic 3-ethylbenzothiazoline-6-sulfonic CD cord-013178-li1x1m25 72 14 acid acid NN cord-013178-li1x1m25 72 15 ) ) -RRB- cord-013178-li1x1m25 72 16 diammonium diammonium NN cord-013178-li1x1m25 72 17 salt salt NN cord-013178-li1x1m25 72 18 ( ( -LRB- cord-013178-li1x1m25 72 19 ABTS ABTS NNP cord-013178-li1x1m25 72 20 , , , cord-013178-li1x1m25 72 21 Sigma Sigma NNP cord-013178-li1x1m25 72 22 - - HYPH cord-013178-li1x1m25 72 23 Aldrich Aldrich NNP cord-013178-li1x1m25 72 24 , , , cord-013178-li1x1m25 72 25 St. St. NNP cord-013178-li1x1m25 72 26 Louis Louis NNP cord-013178-li1x1m25 72 27 , , , cord-013178-li1x1m25 72 28 MO MO NNP cord-013178-li1x1m25 72 29 , , , cord-013178-li1x1m25 72 30 USA USA NNP cord-013178-li1x1m25 72 31 ) ) -RRB- cord-013178-li1x1m25 72 32 buffer buffer NN cord-013178-li1x1m25 72 33 were be VBD cord-013178-li1x1m25 72 34 added add VBN cord-013178-li1x1m25 72 35 to to IN cord-013178-li1x1m25 72 36 each each DT cord-013178-li1x1m25 72 37 well well NN cord-013178-li1x1m25 72 38 of of IN cord-013178-li1x1m25 72 39 the the DT cord-013178-li1x1m25 72 40 plate plate NN cord-013178-li1x1m25 72 41 . . . cord-013178-li1x1m25 73 1 After after IN cord-013178-li1x1m25 73 2 30 30 CD cord-013178-li1x1m25 73 3 min min NN cord-013178-li1x1m25 73 4 , , , cord-013178-li1x1m25 73 5 the the DT cord-013178-li1x1m25 73 6 optical optical JJ cord-013178-li1x1m25 73 7 density density NN cord-013178-li1x1m25 73 8 ( ( -LRB- cord-013178-li1x1m25 73 9 OD OD NNP cord-013178-li1x1m25 73 10 ) ) -RRB- cord-013178-li1x1m25 73 11 of of IN cord-013178-li1x1m25 73 12 each each DT cord-013178-li1x1m25 73 13 well well NN cord-013178-li1x1m25 73 14 was be VBD cord-013178-li1x1m25 73 15 measured measure VBN cord-013178-li1x1m25 73 16 at at IN cord-013178-li1x1m25 73 17 405 405 CD cord-013178-li1x1m25 73 18 nm nm NN cord-013178-li1x1m25 73 19 using use VBG cord-013178-li1x1m25 73 20 a a DT cord-013178-li1x1m25 73 21 SpectraMax SpectraMax NNP cord-013178-li1x1m25 73 22 M5 M5 NNP cord-013178-li1x1m25 73 23 microplate microplate NN cord-013178-li1x1m25 73 24 reader reader NN cord-013178-li1x1m25 73 25 ( ( -LRB- cord-013178-li1x1m25 73 26 Molecular Molecular NNP cord-013178-li1x1m25 73 27 Devices Devices NNPS cord-013178-li1x1m25 73 28 , , , cord-013178-li1x1m25 73 29 San San NNP cord-013178-li1x1m25 73 30 Jose Jose NNP cord-013178-li1x1m25 73 31 , , , cord-013178-li1x1m25 73 32 CA CA NNP cord-013178-li1x1m25 73 33 , , , cord-013178-li1x1m25 73 34 USA USA NNP cord-013178-li1x1m25 73 35 ) ) -RRB- cord-013178-li1x1m25 73 36 . . . cord-013178-li1x1m25 73 37 _SP cord-013178-li1x1m25 74 1 The the DT cord-013178-li1x1m25 74 2 epitope epitope NN cord-013178-li1x1m25 74 3 mapping mapping NN cord-013178-li1x1m25 74 4 of of IN cord-013178-li1x1m25 74 5 these these DT cord-013178-li1x1m25 74 6 mAbs mAbs NNP cord-013178-li1x1m25 74 7 was be VBD cord-013178-li1x1m25 74 8 performed perform VBN cord-013178-li1x1m25 74 9 by by IN cord-013178-li1x1m25 74 10 iELISA iELISA NNP cord-013178-li1x1m25 74 11 [ [ -LRB- cord-013178-li1x1m25 74 12 49 49 CD cord-013178-li1x1m25 74 13 ] ] -RRB- cord-013178-li1x1m25 74 14 using use VBG cord-013178-li1x1m25 74 15 truncated truncate VBN cord-013178-li1x1m25 74 16 peptides peptide NNS cord-013178-li1x1m25 74 17 ( ( -LRB- cord-013178-li1x1m25 74 18 as as IN cord-013178-li1x1m25 74 19 shown show VBN cord-013178-li1x1m25 74 20 in in IN cord-013178-li1x1m25 74 21 Table Table NNP cord-013178-li1x1m25 74 22 1 1 CD cord-013178-li1x1m25 74 23 ) ) -RRB- cord-013178-li1x1m25 74 24 as as IN cord-013178-li1x1m25 74 25 coating coating NN cord-013178-li1x1m25 74 26 antigens antigen NNS cord-013178-li1x1m25 74 27 . . . cord-013178-li1x1m25 75 1 Briefly briefly RB cord-013178-li1x1m25 75 2 , , , cord-013178-li1x1m25 75 3 peptides peptide NNS cord-013178-li1x1m25 75 4 contained contain VBD cord-013178-li1x1m25 75 5 the the DT cord-013178-li1x1m25 75 6 sequence sequence NN cord-013178-li1x1m25 75 7 of of IN cord-013178-li1x1m25 75 8 ORF3 ORF3 NNP cord-013178-li1x1m25 75 9 protein protein NN cord-013178-li1x1m25 75 10 of of IN cord-013178-li1x1m25 75 11 PCV2b pcv2b NN cord-013178-li1x1m25 75 12 between between IN cord-013178-li1x1m25 75 13 residues residue NNS cord-013178-li1x1m25 75 14 35 35 CD cord-013178-li1x1m25 75 15 and and CC cord-013178-li1x1m25 75 16 66 66 CD cord-013178-li1x1m25 75 17 , , , cord-013178-li1x1m25 75 18 associated associated JJ cord-013178-li1x1m25 75 19 10-mer 10-mer CD cord-013178-li1x1m25 75 20 peptides peptide NNS cord-013178-li1x1m25 75 21 , , , cord-013178-li1x1m25 75 22 truncated truncated JJ cord-013178-li1x1m25 75 23 derivatives derivative NNS cord-013178-li1x1m25 75 24 , , , cord-013178-li1x1m25 75 25 and and CC cord-013178-li1x1m25 75 26 the the DT cord-013178-li1x1m25 75 27 control control NN cord-013178-li1x1m25 75 28 ( ( -LRB- cord-013178-li1x1m25 75 29 peptide peptide NN cord-013178-li1x1m25 75 30 C3 C3 NNP cord-013178-li1x1m25 75 31 ) ) -RRB- cord-013178-li1x1m25 75 32 . . . cord-013178-li1x1m25 76 1 Ninety ninety CD cord-013178-li1x1m25 76 2 - - HYPH cord-013178-li1x1m25 76 3 six six CD cord-013178-li1x1m25 76 4 - - HYPH cord-013178-li1x1m25 76 5 well well RB cord-013178-li1x1m25 76 6 Maxisorp Maxisorp NNP cord-013178-li1x1m25 76 7 plates plate NNS cord-013178-li1x1m25 76 8 were be VBD cord-013178-li1x1m25 76 9 coated coat VBN cord-013178-li1x1m25 76 10 with with IN cord-013178-li1x1m25 76 11 each each DT cord-013178-li1x1m25 76 12 peptide peptide NN cord-013178-li1x1m25 76 13 ( ( -LRB- cord-013178-li1x1m25 76 14 5 5 CD cord-013178-li1x1m25 76 15 µg µg NN cord-013178-li1x1m25 76 16 / / SYM cord-013178-li1x1m25 76 17 mL ml NN cord-013178-li1x1m25 76 18 ) ) -RRB- cord-013178-li1x1m25 76 19 in in IN cord-013178-li1x1m25 76 20 bicarbonate bicarbonate NN cord-013178-li1x1m25 76 21 buffer buffer NN cord-013178-li1x1m25 76 22 and and CC cord-013178-li1x1m25 76 23 incubated incubate VBN cord-013178-li1x1m25 76 24 at at IN cord-013178-li1x1m25 76 25 4 4 CD cord-013178-li1x1m25 76 26 • • NN cord-013178-li1x1m25 76 27 C C NNP cord-013178-li1x1m25 76 28 overnight overnight RB cord-013178-li1x1m25 76 29 . . . cord-013178-li1x1m25 77 1 After after IN cord-013178-li1x1m25 77 2 three three CD cord-013178-li1x1m25 77 3 washes wash NNS cord-013178-li1x1m25 77 4 in in IN cord-013178-li1x1m25 77 5 PBST PBST NNP cord-013178-li1x1m25 77 6 , , , cord-013178-li1x1m25 77 7 the the DT cord-013178-li1x1m25 77 8 plates plate NNS cord-013178-li1x1m25 77 9 were be VBD cord-013178-li1x1m25 77 10 blocked block VBN cord-013178-li1x1m25 77 11 with with IN cord-013178-li1x1m25 77 12 the the DT cord-013178-li1x1m25 77 13 blocking block VBG cord-013178-li1x1m25 77 14 buffer buffer NN cord-013178-li1x1m25 77 15 at at IN cord-013178-li1x1m25 77 16 37 37 CD cord-013178-li1x1m25 78 1 • • NNP cord-013178-li1x1m25 78 2 C C NNP cord-013178-li1x1m25 78 3 for for IN cord-013178-li1x1m25 78 4 30 30 CD cord-013178-li1x1m25 78 5 min min NN cord-013178-li1x1m25 78 6 . . . cord-013178-li1x1m25 79 1 After after IN cord-013178-li1x1m25 79 2 washing washing NN cord-013178-li1x1m25 79 3 , , , cord-013178-li1x1m25 79 4 the the DT cord-013178-li1x1m25 79 5 culture culture NN cord-013178-li1x1m25 79 6 supernatant supernatant VBN cord-013178-li1x1m25 79 7 of of IN cord-013178-li1x1m25 79 8 hybridoma hybridoma NN cord-013178-li1x1m25 79 9 was be VBD cord-013178-li1x1m25 79 10 added add VBN cord-013178-li1x1m25 79 11 , , , cord-013178-li1x1m25 79 12 and and CC cord-013178-li1x1m25 79 13 plates plate NNS cord-013178-li1x1m25 79 14 were be VBD cord-013178-li1x1m25 79 15 again again RB cord-013178-li1x1m25 79 16 incubated incubate VBN cord-013178-li1x1m25 79 17 at at IN cord-013178-li1x1m25 79 18 37 37 CD cord-013178-li1x1m25 79 19 • • NN cord-013178-li1x1m25 79 20 C c NN cord-013178-li1x1m25 79 21 for for IN cord-013178-li1x1m25 79 22 2 2 CD cord-013178-li1x1m25 79 23 h. h. NN cord-013178-li1x1m25 80 1 The the DT cord-013178-li1x1m25 80 2 anti anti JJ cord-013178-li1x1m25 80 3 - - JJ cord-013178-li1x1m25 80 4 peptide peptide JJ cord-013178-li1x1m25 80 5 N1 N1 NNP cord-013178-li1x1m25 80 6 mouse mouse NN cord-013178-li1x1m25 80 7 serum serum NN cord-013178-li1x1m25 80 8 ( ( -LRB- cord-013178-li1x1m25 80 9 at at IN cord-013178-li1x1m25 80 10 a a DT cord-013178-li1x1m25 80 11 1:1000 1:1000 CD cord-013178-li1x1m25 80 12 dilution dilution NN cord-013178-li1x1m25 80 13 ) ) -RRB- cord-013178-li1x1m25 80 14 was be VBD cord-013178-li1x1m25 80 15 used use VBN cord-013178-li1x1m25 80 16 as as IN cord-013178-li1x1m25 80 17 the the DT cord-013178-li1x1m25 80 18 positive positive JJ cord-013178-li1x1m25 80 19 control control NN cord-013178-li1x1m25 80 20 , , , cord-013178-li1x1m25 80 21 and and CC cord-013178-li1x1m25 80 22 the the DT cord-013178-li1x1m25 80 23 anti anti JJ cord-013178-li1x1m25 80 24 - - JJ cord-013178-li1x1m25 80 25 ORF2 orf2 JJ cord-013178-li1x1m25 80 26 ( ( -LRB- cord-013178-li1x1m25 80 27 peptide peptide NN cord-013178-li1x1m25 80 28 C3 c3 NN cord-013178-li1x1m25 80 29 ) ) -RRB- cord-013178-li1x1m25 80 30 mAb mab NN cord-013178-li1x1m25 80 31 ( ( -LRB- cord-013178-li1x1m25 80 32 1H3 1h3 CD cord-013178-li1x1m25 80 33 ) ) -RRB- cord-013178-li1x1m25 80 34 was be VBD cord-013178-li1x1m25 80 35 served serve VBN cord-013178-li1x1m25 80 36 as as IN cord-013178-li1x1m25 80 37 the the DT cord-013178-li1x1m25 80 38 negative negative JJ cord-013178-li1x1m25 80 39 control control NN cord-013178-li1x1m25 80 40 . . . cord-013178-li1x1m25 81 1 After after IN cord-013178-li1x1m25 81 2 rinsing rinse VBG cord-013178-li1x1m25 81 3 three three CD cord-013178-li1x1m25 81 4 times time NNS cord-013178-li1x1m25 81 5 with with IN cord-013178-li1x1m25 81 6 PBST PBST NNP cord-013178-li1x1m25 81 7 , , , cord-013178-li1x1m25 81 8 the the DT cord-013178-li1x1m25 81 9 secondary secondary JJ cord-013178-li1x1m25 81 10 antibody antibody NN cord-013178-li1x1m25 81 11 solution solution NN cord-013178-li1x1m25 81 12 was be VBD cord-013178-li1x1m25 81 13 applied apply VBN cord-013178-li1x1m25 81 14 to to IN cord-013178-li1x1m25 81 15 the the DT cord-013178-li1x1m25 81 16 appropriate appropriate JJ cord-013178-li1x1m25 81 17 wells well NNS cord-013178-li1x1m25 81 18 . . . cord-013178-li1x1m25 82 1 Peroxidase peroxidase NN cord-013178-li1x1m25 82 2 - - HYPH cord-013178-li1x1m25 82 3 conjugated conjugate VBN cord-013178-li1x1m25 82 4 goat goat NN cord-013178-li1x1m25 82 5 anti anti JJ cord-013178-li1x1m25 82 6 - - JJ cord-013178-li1x1m25 82 7 mouse mouse JJ cord-013178-li1x1m25 82 8 IgG IgG NNP cord-013178-li1x1m25 82 9 ( ( -LRB- cord-013178-li1x1m25 82 10 subclasses subclass NNS cord-013178-li1x1m25 82 11 1 1 CD cord-013178-li1x1m25 82 12 + + SYM cord-013178-li1x1m25 82 13 2a 2a CD cord-013178-li1x1m25 82 14 + + SYM cord-013178-li1x1m25 82 15 2b 2b CD cord-013178-li1x1m25 83 1 + + CC cord-013178-li1x1m25 83 2 3 3 CD cord-013178-li1x1m25 84 1 , , , cord-013178-li1x1m25 84 2 Fcγ Fcγ NNP cord-013178-li1x1m25 84 3 , , , cord-013178-li1x1m25 84 4 Jackson Jackson NNP cord-013178-li1x1m25 84 5 ImmunoResearch ImmunoResearch NNP cord-013178-li1x1m25 84 6 , , , cord-013178-li1x1m25 84 7 West West NNP cord-013178-li1x1m25 84 8 Grove Grove NNP cord-013178-li1x1m25 84 9 , , , cord-013178-li1x1m25 84 10 PA PA NNP cord-013178-li1x1m25 84 11 , , , cord-013178-li1x1m25 84 12 USA USA NNP cord-013178-li1x1m25 84 13 ) ) -RRB- cord-013178-li1x1m25 84 14 was be VBD cord-013178-li1x1m25 84 15 used use VBN cord-013178-li1x1m25 84 16 at at IN cord-013178-li1x1m25 84 17 a a DT cord-013178-li1x1m25 84 18 1:2500 1:2500 CD cord-013178-li1x1m25 84 19 dilution dilution NN cord-013178-li1x1m25 84 20 for for IN cord-013178-li1x1m25 84 21 binding bind VBG cord-013178-li1x1m25 84 22 to to IN cord-013178-li1x1m25 84 23 the the DT cord-013178-li1x1m25 84 24 mAb mAb NNP cord-013178-li1x1m25 84 25 7D3 7D3 NNP cord-013178-li1x1m25 84 26 . . . cord-013178-li1x1m25 85 1 HRP HRP NNP cord-013178-li1x1m25 85 2 - - HYPH cord-013178-li1x1m25 85 3 conjugated conjugate VBN cord-013178-li1x1m25 85 4 goat goat NN cord-013178-li1x1m25 85 5 anti anti JJ cord-013178-li1x1m25 85 6 - - JJ cord-013178-li1x1m25 85 7 mouse mouse JJ cord-013178-li1x1m25 85 8 lambda lambda NN cord-013178-li1x1m25 85 9 light light NN cord-013178-li1x1m25 85 10 chain chain NN cord-013178-li1x1m25 85 11 ( ( -LRB- cord-013178-li1x1m25 85 12 Novus Novus NNP cord-013178-li1x1m25 85 13 , , , cord-013178-li1x1m25 85 14 Saint Saint NNP cord-013178-li1x1m25 85 15 Charles Charles NNP cord-013178-li1x1m25 85 16 , , , cord-013178-li1x1m25 85 17 MO MO NNP cord-013178-li1x1m25 85 18 , , , cord-013178-li1x1m25 85 19 USA USA NNP cord-013178-li1x1m25 85 20 ) ) -RRB- cord-013178-li1x1m25 85 21 was be VBD cord-013178-li1x1m25 85 22 used use VBN cord-013178-li1x1m25 85 23 at at IN cord-013178-li1x1m25 85 24 a a DT cord-013178-li1x1m25 85 25 1:5000 1:5000 CD cord-013178-li1x1m25 85 26 dilution dilution NN cord-013178-li1x1m25 85 27 for for IN cord-013178-li1x1m25 85 28 binding bind VBG cord-013178-li1x1m25 85 29 to to IN cord-013178-li1x1m25 85 30 the the DT cord-013178-li1x1m25 85 31 mAb mAb NNP cord-013178-li1x1m25 85 32 6D10 6d10 CD cord-013178-li1x1m25 85 33 . . . cord-013178-li1x1m25 86 1 After after IN cord-013178-li1x1m25 86 2 1 1 CD cord-013178-li1x1m25 86 3 h h NN cord-013178-li1x1m25 86 4 , , , cord-013178-li1x1m25 86 5 the the DT cord-013178-li1x1m25 86 6 plates plate NNS cord-013178-li1x1m25 86 7 were be VBD cord-013178-li1x1m25 86 8 washed wash VBN cord-013178-li1x1m25 86 9 three three CD cord-013178-li1x1m25 86 10 times time NNS cord-013178-li1x1m25 86 11 . . . cord-013178-li1x1m25 87 1 The the DT cord-013178-li1x1m25 87 2 colorimetric colorimetric JJ cord-013178-li1x1m25 87 3 reaction reaction NN cord-013178-li1x1m25 87 4 was be VBD cord-013178-li1x1m25 87 5 developed develop VBN cord-013178-li1x1m25 87 6 by by IN cord-013178-li1x1m25 87 7 using use VBG cord-013178-li1x1m25 87 8 the the DT cord-013178-li1x1m25 87 9 ABTS ABTS NNP cord-013178-li1x1m25 87 10 solution solution NN cord-013178-li1x1m25 87 11 . . . cord-013178-li1x1m25 88 1 Following follow VBG cord-013178-li1x1m25 88 2 a a DT cord-013178-li1x1m25 88 3 30-min 30-min CD cord-013178-li1x1m25 88 4 incubation incubation NN cord-013178-li1x1m25 88 5 , , , cord-013178-li1x1m25 88 6 the the DT cord-013178-li1x1m25 88 7 OD OD NNP cord-013178-li1x1m25 88 8 was be VBD cord-013178-li1x1m25 88 9 measured measure VBN cord-013178-li1x1m25 88 10 at at IN cord-013178-li1x1m25 88 11 405 405 CD cord-013178-li1x1m25 88 12 nm nm NN cord-013178-li1x1m25 88 13 . . . cord-013178-li1x1m25 89 1 Both both DT cord-013178-li1x1m25 89 2 of of IN cord-013178-li1x1m25 89 3 the the DT cord-013178-li1x1m25 89 4 isotype isotype NN cord-013178-li1x1m25 89 5 and and CC cord-013178-li1x1m25 89 6 molecular molecular JJ cord-013178-li1x1m25 89 7 weight weight NN cord-013178-li1x1m25 89 8 of of IN cord-013178-li1x1m25 89 9 the the DT cord-013178-li1x1m25 89 10 mAb mAb NNS cord-013178-li1x1m25 89 11 6D10 6d10 CD cord-013178-li1x1m25 89 12 were be VBD cord-013178-li1x1m25 89 13 determined determine VBN cord-013178-li1x1m25 89 14 by by IN cord-013178-li1x1m25 89 15 Western western JJ cord-013178-li1x1m25 89 16 blotting blotting NN cord-013178-li1x1m25 89 17 . . . cord-013178-li1x1m25 90 1 The the DT cord-013178-li1x1m25 90 2 culture culture NN cord-013178-li1x1m25 90 3 supernatant supernatant JJ cord-013178-li1x1m25 90 4 of of IN cord-013178-li1x1m25 90 5 the the DT cord-013178-li1x1m25 90 6 hybridoma hybridoma NN cord-013178-li1x1m25 90 7 ( ( -LRB- cord-013178-li1x1m25 90 8 clone clone NN cord-013178-li1x1m25 90 9 6D10 6d10 CD cord-013178-li1x1m25 90 10 ) ) -RRB- cord-013178-li1x1m25 90 11 was be VBD cord-013178-li1x1m25 90 12 separated separate VBN cord-013178-li1x1m25 90 13 by by IN cord-013178-li1x1m25 90 14 BoltTM BoltTM NNP cord-013178-li1x1m25 90 15 Bis Bis NNP cord-013178-li1x1m25 90 16 - - HYPH cord-013178-li1x1m25 90 17 Tris Tris NNP cord-013178-li1x1m25 90 18 Plus plus CC cord-013178-li1x1m25 90 19 Gel Gel NNP cord-013178-li1x1m25 90 20 ( ( -LRB- cord-013178-li1x1m25 90 21 Invitrogen Invitrogen NNP cord-013178-li1x1m25 90 22 , , , cord-013178-li1x1m25 90 23 Carlsbad Carlsbad NNP cord-013178-li1x1m25 90 24 , , , cord-013178-li1x1m25 90 25 CA CA NNP cord-013178-li1x1m25 90 26 , , , cord-013178-li1x1m25 90 27 USA USA NNP cord-013178-li1x1m25 90 28 ) ) -RRB- cord-013178-li1x1m25 90 29 . . . cord-013178-li1x1m25 91 1 One one CD cord-013178-li1x1m25 91 2 gel gel NN cord-013178-li1x1m25 91 3 was be VBD cord-013178-li1x1m25 91 4 stained stain VBN cord-013178-li1x1m25 91 5 with with IN cord-013178-li1x1m25 91 6 FaStain FaStain NNP cord-013178-li1x1m25 91 7 Protein Protein NNP cord-013178-li1x1m25 91 8 Staining Staining NNP cord-013178-li1x1m25 91 9 Solution Solution NNP cord-013178-li1x1m25 91 10 ( ( -LRB- cord-013178-li1x1m25 91 11 Yeastern Yeastern NNP cord-013178-li1x1m25 91 12 Biotech Biotech NNP cord-013178-li1x1m25 91 13 , , , cord-013178-li1x1m25 91 14 Taiwan Taiwan NNP cord-013178-li1x1m25 91 15 ) ) -RRB- cord-013178-li1x1m25 91 16 . . . cord-013178-li1x1m25 92 1 The the DT cord-013178-li1x1m25 92 2 other other JJ cord-013178-li1x1m25 92 3 gel gel NN cord-013178-li1x1m25 92 4 was be VBD cord-013178-li1x1m25 92 5 transferred transfer VBN cord-013178-li1x1m25 92 6 to to IN cord-013178-li1x1m25 92 7 the the DT cord-013178-li1x1m25 92 8 polyvinylidene polyvinylidene NN cord-013178-li1x1m25 92 9 difluoride difluoride NN cord-013178-li1x1m25 92 10 ( ( -LRB- cord-013178-li1x1m25 92 11 PVDF PVDF NNP cord-013178-li1x1m25 92 12 ) ) -RRB- cord-013178-li1x1m25 92 13 membranes membrane NNS cord-013178-li1x1m25 92 14 ( ( -LRB- cord-013178-li1x1m25 92 15 Millipore Millipore NNP cord-013178-li1x1m25 92 16 , , , cord-013178-li1x1m25 92 17 Burlington Burlington NNP cord-013178-li1x1m25 92 18 , , , cord-013178-li1x1m25 92 19 MA MA NNP cord-013178-li1x1m25 92 20 , , , cord-013178-li1x1m25 92 21 USA USA NNP cord-013178-li1x1m25 92 22 ) ) -RRB- cord-013178-li1x1m25 92 23 with with IN cord-013178-li1x1m25 92 24 Fast fast JJ cord-013178-li1x1m25 92 25 Semi Semi NNP cord-013178-li1x1m25 92 26 - - HYPH cord-013178-li1x1m25 92 27 Dry dry JJ cord-013178-li1x1m25 92 28 transfer transfer NN cord-013178-li1x1m25 92 29 buffer buffer NN cord-013178-li1x1m25 92 30 ( ( -LRB- cord-013178-li1x1m25 92 31 Thermo Thermo NNP cord-013178-li1x1m25 92 32 , , , cord-013178-li1x1m25 92 33 Waltham Waltham NNP cord-013178-li1x1m25 92 34 , , , cord-013178-li1x1m25 92 35 MA MA NNP cord-013178-li1x1m25 92 36 , , , cord-013178-li1x1m25 92 37 USA USA NNP cord-013178-li1x1m25 92 38 ) ) -RRB- cord-013178-li1x1m25 92 39 by by IN cord-013178-li1x1m25 92 40 using use VBG cord-013178-li1x1m25 92 41 a a DT cord-013178-li1x1m25 92 42 Yrdimes Yrdimes NNP cord-013178-li1x1m25 92 43 Semi semi JJ cord-013178-li1x1m25 92 44 - - JJ cord-013178-li1x1m25 92 45 dry dry JJ cord-013178-li1x1m25 92 46 transfer transfer NN cord-013178-li1x1m25 92 47 system system NN cord-013178-li1x1m25 92 48 ( ( -LRB- cord-013178-li1x1m25 92 49 Wealtec Wealtec NNP cord-013178-li1x1m25 92 50 , , , cord-013178-li1x1m25 92 51 New New NNP cord-013178-li1x1m25 92 52 Taipei Taipei NNP cord-013178-li1x1m25 92 53 City City NNP cord-013178-li1x1m25 92 54 , , , cord-013178-li1x1m25 92 55 Taiwan Taiwan NNP cord-013178-li1x1m25 92 56 ) ) -RRB- cord-013178-li1x1m25 92 57 . . . cord-013178-li1x1m25 93 1 The the DT cord-013178-li1x1m25 93 2 membrane membrane NN cord-013178-li1x1m25 93 3 was be VBD cord-013178-li1x1m25 93 4 blocked block VBN cord-013178-li1x1m25 93 5 with with IN cord-013178-li1x1m25 93 6 the the DT cord-013178-li1x1m25 93 7 blocking block VBG cord-013178-li1x1m25 93 8 buffer buffer NN cord-013178-li1x1m25 93 9 for for IN cord-013178-li1x1m25 93 10 30 30 CD cord-013178-li1x1m25 93 11 min min NN cord-013178-li1x1m25 93 12 at at IN cord-013178-li1x1m25 93 13 37 37 CD cord-013178-li1x1m25 93 14 • • NN cord-013178-li1x1m25 93 15 C. C. NNP cord-013178-li1x1m25 93 16 After after IN cord-013178-li1x1m25 93 17 three three CD cord-013178-li1x1m25 93 18 washes wash NNS cord-013178-li1x1m25 93 19 in in IN cord-013178-li1x1m25 93 20 PBST PBST NNP cord-013178-li1x1m25 93 21 , , , cord-013178-li1x1m25 93 22 each each DT cord-013178-li1x1m25 93 23 strip strip NN cord-013178-li1x1m25 93 24 of of IN cord-013178-li1x1m25 93 25 the the DT cord-013178-li1x1m25 93 26 membrane membrane NN cord-013178-li1x1m25 93 27 was be VBD cord-013178-li1x1m25 93 28 incubated incubate VBN cord-013178-li1x1m25 93 29 with with IN cord-013178-li1x1m25 93 30 one one CD cord-013178-li1x1m25 93 31 appropriate appropriate JJ cord-013178-li1x1m25 93 32 horseradish horseradish NN cord-013178-li1x1m25 93 33 peroxidase peroxidase NN cord-013178-li1x1m25 93 34 ( ( -LRB- cord-013178-li1x1m25 93 35 HRP)-conjugated hrp)-conjugate VBN cord-013178-li1x1m25 93 36 antibody antibody NN cord-013178-li1x1m25 93 37 to to TO cord-013178-li1x1m25 93 38 detect detect VB cord-013178-li1x1m25 93 39 mAb mab NN cord-013178-li1x1m25 93 40 at at IN cord-013178-li1x1m25 93 41 4 4 CD cord-013178-li1x1m25 93 42 • • NN cord-013178-li1x1m25 93 43 C c NN cord-013178-li1x1m25 93 44 overnight overnight RB cord-013178-li1x1m25 94 1 and and CC cord-013178-li1x1m25 94 2 then then RB cord-013178-li1x1m25 94 3 at at IN cord-013178-li1x1m25 94 4 room room NN cord-013178-li1x1m25 94 5 temperature temperature NN cord-013178-li1x1m25 94 6 for for IN cord-013178-li1x1m25 94 7 2 2 CD cord-013178-li1x1m25 94 8 h. h. NNP cord-013178-li1x1m25 94 9 Peroxidase peroxidase NN cord-013178-li1x1m25 94 10 - - HYPH cord-013178-li1x1m25 94 11 conjugated conjugate VBN cord-013178-li1x1m25 94 12 goat goat NN cord-013178-li1x1m25 94 13 anti anti JJ cord-013178-li1x1m25 94 14 - - JJ cord-013178-li1x1m25 94 15 mouse mouse NNP cord-013178-li1x1m25 94 16 IgA IgA NNP cord-013178-li1x1m25 94 17 / / SYM cord-013178-li1x1m25 94 18 IgG IgG NNP cord-013178-li1x1m25 94 19 / / SYM cord-013178-li1x1m25 94 20 IgM IgM NNP cord-013178-li1x1m25 94 21 , , , cord-013178-li1x1m25 94 22 H H NNP cord-013178-li1x1m25 94 23 / / SYM cord-013178-li1x1m25 94 24 L L NNP cord-013178-li1x1m25 94 25 chain chain NN cord-013178-li1x1m25 94 26 ( ( -LRB- cord-013178-li1x1m25 94 27 Novus Novus NNP cord-013178-li1x1m25 94 28 , , , cord-013178-li1x1m25 94 29 Saint Saint NNP cord-013178-li1x1m25 94 30 Charles Charles NNP cord-013178-li1x1m25 94 31 , , , cord-013178-li1x1m25 94 32 MO MO NNP cord-013178-li1x1m25 94 33 , , , cord-013178-li1x1m25 94 34 USA USA NNP cord-013178-li1x1m25 94 35 ) ) -RRB- cord-013178-li1x1m25 94 36 at at IN cord-013178-li1x1m25 94 37 1:2500 1:2500 CD cord-013178-li1x1m25 95 1 , , , cord-013178-li1x1m25 95 2 HRP HRP NNP cord-013178-li1x1m25 95 3 - - HYPH cord-013178-li1x1m25 95 4 conjugated conjugate VBN cord-013178-li1x1m25 95 5 goat goat NN cord-013178-li1x1m25 95 6 anti anti JJ cord-013178-li1x1m25 95 7 - - JJ cord-013178-li1x1m25 95 8 mouse mouse JJ cord-013178-li1x1m25 95 9 IgA IgA NNP cord-013178-li1x1m25 95 10 ( ( -LRB- cord-013178-li1x1m25 95 11 Southern Southern NNP cord-013178-li1x1m25 95 12 Biotechnology Biotechnology NNP cord-013178-li1x1m25 95 13 , , , cord-013178-li1x1m25 95 14 Birmingham Birmingham NNP cord-013178-li1x1m25 95 15 , , , cord-013178-li1x1m25 95 16 AL AL NNP cord-013178-li1x1m25 95 17 , , , cord-013178-li1x1m25 95 18 USA USA NNP cord-013178-li1x1m25 95 19 ) ) -RRB- cord-013178-li1x1m25 95 20 at at IN cord-013178-li1x1m25 95 21 1:2500 1:2500 CD cord-013178-li1x1m25 95 22 , , , cord-013178-li1x1m25 95 23 HRP HRP NNP cord-013178-li1x1m25 95 24 - - HYPH cord-013178-li1x1m25 95 25 conjugated conjugate VBN cord-013178-li1x1m25 95 26 goat goat NN cord-013178-li1x1m25 95 27 anti anti JJ cord-013178-li1x1m25 95 28 - - JJ cord-013178-li1x1m25 95 29 mouse mouse JJ cord-013178-li1x1m25 95 30 lambda lambda NN cord-013178-li1x1m25 95 31 light light NN cord-013178-li1x1m25 95 32 chain chain NN cord-013178-li1x1m25 95 33 ( ( -LRB- cord-013178-li1x1m25 95 34 Novus Novus NNP cord-013178-li1x1m25 95 35 , , , cord-013178-li1x1m25 95 36 Saint Saint NNP cord-013178-li1x1m25 95 37 Charles Charles NNP cord-013178-li1x1m25 95 38 , , , cord-013178-li1x1m25 95 39 MO MO NNP cord-013178-li1x1m25 95 40 , , , cord-013178-li1x1m25 95 41 USA USA NNP cord-013178-li1x1m25 95 42 ) ) -RRB- cord-013178-li1x1m25 95 43 at at IN cord-013178-li1x1m25 95 44 1:5000 1:5000 NNPS cord-013178-li1x1m25 95 45 , , , cord-013178-li1x1m25 95 46 HRP HRP NNP cord-013178-li1x1m25 95 47 - - HYPH cord-013178-li1x1m25 95 48 conjugated conjugate VBN cord-013178-li1x1m25 95 49 goat goat NN cord-013178-li1x1m25 95 50 anti anti JJ cord-013178-li1x1m25 95 51 - - JJ cord-013178-li1x1m25 95 52 mouse mouse JJ cord-013178-li1x1m25 95 53 IgM IgM NNP cord-013178-li1x1m25 95 54 , , , cord-013178-li1x1m25 95 55 µ µ NNP cord-013178-li1x1m25 95 56 chain chain NN cord-013178-li1x1m25 96 1 ( ( -LRB- cord-013178-li1x1m25 96 2 Jackson Jackson NNP cord-013178-li1x1m25 96 3 Immunoresearch Immunoresearch NNP cord-013178-li1x1m25 96 4 , , , cord-013178-li1x1m25 96 5 West West NNP cord-013178-li1x1m25 96 6 Grove Grove NNP cord-013178-li1x1m25 96 7 , , , cord-013178-li1x1m25 96 8 PA PA NNP cord-013178-li1x1m25 96 9 , , , cord-013178-li1x1m25 96 10 USA USA NNP cord-013178-li1x1m25 96 11 ) ) -RRB- cord-013178-li1x1m25 96 12 at at IN cord-013178-li1x1m25 96 13 1:5000 1:5000 NNPS cord-013178-li1x1m25 96 14 , , , cord-013178-li1x1m25 96 15 and and CC cord-013178-li1x1m25 96 16 HRP HRP NNP cord-013178-li1x1m25 96 17 - - HYPH cord-013178-li1x1m25 96 18 conjugated conjugate VBN cord-013178-li1x1m25 96 19 goat goat NN cord-013178-li1x1m25 96 20 anti anti JJ cord-013178-li1x1m25 96 21 - - JJ cord-013178-li1x1m25 96 22 mouse mouse JJ cord-013178-li1x1m25 96 23 IgG IgG NNP cord-013178-li1x1m25 96 24 ( ( -LRB- cord-013178-li1x1m25 96 25 subclasses subclass NNS cord-013178-li1x1m25 96 26 1 1 CD cord-013178-li1x1m25 96 27 + + SYM cord-013178-li1x1m25 96 28 2a 2a CD cord-013178-li1x1m25 96 29 + + SYM cord-013178-li1x1m25 96 30 2b 2b CD cord-013178-li1x1m25 97 1 + + SYM cord-013178-li1x1m25 97 2 3 3 CD cord-013178-li1x1m25 97 3 , , , cord-013178-li1x1m25 97 4 Fcγ Fcγ NNP cord-013178-li1x1m25 97 5 , , , cord-013178-li1x1m25 97 6 Jackson Jackson NNP cord-013178-li1x1m25 97 7 Immunoresearch Immunoresearch NNP cord-013178-li1x1m25 97 8 , , , cord-013178-li1x1m25 97 9 West West NNP cord-013178-li1x1m25 97 10 Grove Grove NNP cord-013178-li1x1m25 97 11 , , , cord-013178-li1x1m25 97 12 PA PA NNP cord-013178-li1x1m25 97 13 , , , cord-013178-li1x1m25 97 14 USA USA NNP cord-013178-li1x1m25 97 15 ) ) -RRB- cord-013178-li1x1m25 97 16 at at IN cord-013178-li1x1m25 97 17 1:2500 1:2500 CD cord-013178-li1x1m25 97 18 were be VBD cord-013178-li1x1m25 97 19 used use VBN cord-013178-li1x1m25 97 20 in in IN cord-013178-li1x1m25 97 21 this this DT cord-013178-li1x1m25 97 22 assay assay NN cord-013178-li1x1m25 97 23 . . . cord-013178-li1x1m25 98 1 Then then RB cord-013178-li1x1m25 98 2 , , , cord-013178-li1x1m25 98 3 they -PRON- PRP cord-013178-li1x1m25 98 4 were be VBD cord-013178-li1x1m25 98 5 washed wash VBN cord-013178-li1x1m25 98 6 three three CD cord-013178-li1x1m25 98 7 times time NNS cord-013178-li1x1m25 98 8 with with IN cord-013178-li1x1m25 98 9 PBST PBST NNP cord-013178-li1x1m25 98 10 , , , cord-013178-li1x1m25 98 11 5 5 CD cord-013178-li1x1m25 98 12 min min NN cord-013178-li1x1m25 98 13 each each DT cord-013178-li1x1m25 98 14 wash wash NN cord-013178-li1x1m25 98 15 . . . cord-013178-li1x1m25 99 1 Each each DT cord-013178-li1x1m25 99 2 strip strip NN cord-013178-li1x1m25 99 3 was be VBD cord-013178-li1x1m25 99 4 incubated incubate VBN cord-013178-li1x1m25 99 5 with with IN cord-013178-li1x1m25 99 6 3 3 CD cord-013178-li1x1m25 99 7 , , , cord-013178-li1x1m25 99 8 3 3 CD cord-013178-li1x1m25 99 9 -diaminobenzidine -diaminobenzidine JJ cord-013178-li1x1m25 99 10 ( ( -LRB- cord-013178-li1x1m25 99 11 DAB dab NN cord-013178-li1x1m25 99 12 , , , cord-013178-li1x1m25 99 13 horseradish horseradish NN cord-013178-li1x1m25 99 14 peroxidase peroxidase NN cord-013178-li1x1m25 99 15 substrate substrate NN cord-013178-li1x1m25 99 16 , , , cord-013178-li1x1m25 99 17 Millipore Millipore NNP cord-013178-li1x1m25 99 18 , , , cord-013178-li1x1m25 99 19 Burlington Burlington NNP cord-013178-li1x1m25 99 20 , , , cord-013178-li1x1m25 99 21 MA MA NNP cord-013178-li1x1m25 99 22 , , , cord-013178-li1x1m25 99 23 USA USA NNP cord-013178-li1x1m25 99 24 ) ) -RRB- cord-013178-li1x1m25 99 25 buffer buffer NN cord-013178-li1x1m25 99 26 for for IN cord-013178-li1x1m25 99 27 5 5 CD cord-013178-li1x1m25 99 28 min min NN cord-013178-li1x1m25 99 29 . . . cord-013178-li1x1m25 100 1 Protein protein NN cord-013178-li1x1m25 100 2 molecular molecular JJ cord-013178-li1x1m25 100 3 weight weight NN cord-013178-li1x1m25 100 4 markers marker NNS cord-013178-li1x1m25 100 5 were be VBD cord-013178-li1x1m25 100 6 included include VBN cord-013178-li1x1m25 100 7 in in IN cord-013178-li1x1m25 100 8 each each DT cord-013178-li1x1m25 100 9 Western western JJ cord-013178-li1x1m25 100 10 blot blot NN cord-013178-li1x1m25 100 11 analysis analysis NN cord-013178-li1x1m25 100 12 . . . cord-013178-li1x1m25 101 1 After after IN cord-013178-li1x1m25 101 2 revelation revelation NN cord-013178-li1x1m25 101 3 with with IN cord-013178-li1x1m25 101 4 the the DT cord-013178-li1x1m25 101 5 DAB dab NN cord-013178-li1x1m25 101 6 substrate substrate NN cord-013178-li1x1m25 101 7 , , , cord-013178-li1x1m25 101 8 the the DT cord-013178-li1x1m25 101 9 target target NN cord-013178-li1x1m25 101 10 protein protein NN cord-013178-li1x1m25 101 11 was be VBD cord-013178-li1x1m25 101 12 visualized visualize VBN cord-013178-li1x1m25 101 13 and and CC cord-013178-li1x1m25 101 14 calculated calculate VBD cord-013178-li1x1m25 101 15 the the DT cord-013178-li1x1m25 101 16 molecular molecular JJ cord-013178-li1x1m25 101 17 weight weight NN cord-013178-li1x1m25 101 18 by by IN cord-013178-li1x1m25 101 19 using use VBG cord-013178-li1x1m25 101 20 molecular molecular JJ cord-013178-li1x1m25 101 21 weight weight NN cord-013178-li1x1m25 101 22 markers marker NNS cord-013178-li1x1m25 101 23 . . . cord-013178-li1x1m25 102 1 Homemade homemade JJ cord-013178-li1x1m25 102 2 immunofluorescence immunofluorescence NN cord-013178-li1x1m25 102 3 assay assay NN cord-013178-li1x1m25 102 4 ( ( -LRB- cord-013178-li1x1m25 102 5 IFA IFA NNP cord-013178-li1x1m25 102 6 ) ) -RRB- cord-013178-li1x1m25 102 7 slides slide NNS cord-013178-li1x1m25 102 8 were be VBD cord-013178-li1x1m25 102 9 performed perform VBN cord-013178-li1x1m25 102 10 as as IN cord-013178-li1x1m25 102 11 previously previously RB cord-013178-li1x1m25 102 12 described describe VBN cord-013178-li1x1m25 102 13 [ [ -LRB- cord-013178-li1x1m25 102 14 49 49 CD cord-013178-li1x1m25 102 15 ] ] -RRB- cord-013178-li1x1m25 102 16 . . . cord-013178-li1x1m25 103 1 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 103 2 peripheral peripheral JJ cord-013178-li1x1m25 103 3 blood blood NN cord-013178-li1x1m25 103 4 mononuclear mononuclear NN cord-013178-li1x1m25 103 5 cells cell NNS cord-013178-li1x1m25 103 6 ( ( -LRB- cord-013178-li1x1m25 103 7 PBMCs pbmc NNS cord-013178-li1x1m25 103 8 ) ) -RRB- cord-013178-li1x1m25 103 9 were be VBD cord-013178-li1x1m25 103 10 collected collect VBN cord-013178-li1x1m25 103 11 from from IN cord-013178-li1x1m25 103 12 the the DT cord-013178-li1x1m25 103 13 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 103 14 conventional conventional JJ cord-013178-li1x1m25 103 15 piglet piglet NN cord-013178-li1x1m25 103 16 . . . cord-013178-li1x1m25 104 1 The the DT cord-013178-li1x1m25 104 2 pig pig NN cord-013178-li1x1m25 104 3 serum serum NN cord-013178-li1x1m25 104 4 was be VBD cord-013178-li1x1m25 104 5 detected detect VBN cord-013178-li1x1m25 104 6 as as IN cord-013178-li1x1m25 104 7 seropositive seropositive JJ cord-013178-li1x1m25 104 8 for for IN cord-013178-li1x1m25 104 9 PCV2 PCV2 NNP cord-013178-li1x1m25 104 10 by by IN cord-013178-li1x1m25 104 11 anti anti JJ cord-013178-li1x1m25 104 12 - - JJ cord-013178-li1x1m25 104 13 capsid capsid JJ cord-013178-li1x1m25 104 14 peptide peptide NN cord-013178-li1x1m25 104 15 ( ( -LRB- cord-013178-li1x1m25 104 16 C3 c3 NN cord-013178-li1x1m25 104 17 ) ) -RRB- cord-013178-li1x1m25 104 18 specific specific JJ cord-013178-li1x1m25 104 19 immunoassay immunoassay XX cord-013178-li1x1m25 104 20 [ [ -LRB- cord-013178-li1x1m25 104 21 48 48 CD cord-013178-li1x1m25 104 22 ] ] -RRB- cord-013178-li1x1m25 104 23 . . . cord-013178-li1x1m25 105 1 The the DT cord-013178-li1x1m25 105 2 whole whole JJ cord-013178-li1x1m25 105 3 blood blood NN cord-013178-li1x1m25 105 4 sample sample NN cord-013178-li1x1m25 105 5 was be VBD cord-013178-li1x1m25 105 6 confirmed confirm VBN cord-013178-li1x1m25 105 7 as as IN cord-013178-li1x1m25 105 8 positive positive JJ cord-013178-li1x1m25 105 9 for for IN cord-013178-li1x1m25 105 10 PCV2 pcv2 JJ cord-013178-li1x1m25 105 11 antigen antigen NN cord-013178-li1x1m25 105 12 by by IN cord-013178-li1x1m25 105 13 using use VBG cord-013178-li1x1m25 105 14 the the DT cord-013178-li1x1m25 105 15 INGEZIM INGEZIM NNP cord-013178-li1x1m25 105 16 PCV PCV NNP cord-013178-li1x1m25 105 17 DAS DAS NNP cord-013178-li1x1m25 105 18 kit kit NN cord-013178-li1x1m25 105 19 ( ( -LRB- cord-013178-li1x1m25 105 20 Ingenasa Ingenasa NNP cord-013178-li1x1m25 105 21 , , , cord-013178-li1x1m25 105 22 Madrid Madrid NNP cord-013178-li1x1m25 105 23 , , , cord-013178-li1x1m25 105 24 Spain Spain NNP cord-013178-li1x1m25 105 25 ) ) -RRB- cord-013178-li1x1m25 105 26 . . . cord-013178-li1x1m25 106 1 Briefly briefly RB cord-013178-li1x1m25 106 2 , , , cord-013178-li1x1m25 106 3 PBMCs pbmc NNS cord-013178-li1x1m25 106 4 were be VBD cord-013178-li1x1m25 106 5 isolated isolate VBN cord-013178-li1x1m25 106 6 from from IN cord-013178-li1x1m25 106 7 ethylenediaminetetraacetic ethylenediaminetetraacetic JJ cord-013178-li1x1m25 106 8 acid acid NN cord-013178-li1x1m25 106 9 ( ( -LRB- cord-013178-li1x1m25 106 10 EDTA)-treated edta)-treated JJ cord-013178-li1x1m25 106 11 whole whole JJ cord-013178-li1x1m25 106 12 blood blood NN cord-013178-li1x1m25 106 13 samples sample NNS cord-013178-li1x1m25 106 14 by by IN cord-013178-li1x1m25 107 1 Ficoll Ficoll NNP cord-013178-li1x1m25 107 2 - - HYPH cord-013178-li1x1m25 107 3 Paque Paque NNP cord-013178-li1x1m25 107 4 Plus Plus NNP cord-013178-li1x1m25 107 5 ( ( -LRB- cord-013178-li1x1m25 107 6 GE GE NNP cord-013178-li1x1m25 107 7 Healthcare Healthcare NNP cord-013178-li1x1m25 107 8 Bio Bio NNP cord-013178-li1x1m25 107 9 - - HYPH cord-013178-li1x1m25 107 10 Sciences Sciences NNPS cord-013178-li1x1m25 107 11 , , , cord-013178-li1x1m25 107 12 Uppsala Uppsala NNP cord-013178-li1x1m25 107 13 , , , cord-013178-li1x1m25 107 14 Sweden Sweden NNP cord-013178-li1x1m25 107 15 ) ) -RRB- cord-013178-li1x1m25 107 16 and and CC cord-013178-li1x1m25 107 17 according accord VBG cord-013178-li1x1m25 107 18 to to IN cord-013178-li1x1m25 107 19 the the DT cord-013178-li1x1m25 107 20 manufacturer manufacturer NN cord-013178-li1x1m25 107 21 's 's POS cord-013178-li1x1m25 107 22 instructions instruction NNS cord-013178-li1x1m25 107 23 . . . cord-013178-li1x1m25 108 1 Then then RB cord-013178-li1x1m25 108 2 , , , cord-013178-li1x1m25 108 3 mononuclear mononuclear NN cord-013178-li1x1m25 108 4 cells cell NNS cord-013178-li1x1m25 108 5 were be VBD cord-013178-li1x1m25 108 6 drawn draw VBN cord-013178-li1x1m25 108 7 from from IN cord-013178-li1x1m25 108 8 the the DT cord-013178-li1x1m25 108 9 interface interface NN cord-013178-li1x1m25 108 10 of of IN cord-013178-li1x1m25 108 11 the the DT cord-013178-li1x1m25 108 12 Ficoll Ficoll NNP cord-013178-li1x1m25 108 13 - - HYPH cord-013178-li1x1m25 108 14 Paque Paque NNP cord-013178-li1x1m25 108 15 Plus Plus NNP cord-013178-li1x1m25 108 16 - - HYPH cord-013178-li1x1m25 108 17 containing contain VBG cord-013178-li1x1m25 108 18 tube tube NN cord-013178-li1x1m25 108 19 and and CC cord-013178-li1x1m25 108 20 resuspended resuspend VBD cord-013178-li1x1m25 108 21 in in IN cord-013178-li1x1m25 108 22 45 45 CD cord-013178-li1x1m25 108 23 mL ml NN cord-013178-li1x1m25 108 24 PBS PBS NNP cord-013178-li1x1m25 108 25 for for IN cord-013178-li1x1m25 108 26 washing washing NN cord-013178-li1x1m25 108 27 and and CC cord-013178-li1x1m25 108 28 centrifugation centrifugation NN cord-013178-li1x1m25 108 29 ( ( -LRB- cord-013178-li1x1m25 108 30 100× 100× CD cord-013178-li1x1m25 108 31 g g NN cord-013178-li1x1m25 108 32 for for IN cord-013178-li1x1m25 108 33 10 10 CD cord-013178-li1x1m25 108 34 min min NN cord-013178-li1x1m25 108 35 at at IN cord-013178-li1x1m25 108 36 4 4 CD cord-013178-li1x1m25 108 37 • • NN cord-013178-li1x1m25 108 38 C C NNP cord-013178-li1x1m25 108 39 ) ) -RRB- cord-013178-li1x1m25 108 40 . . . cord-013178-li1x1m25 109 1 After after IN cord-013178-li1x1m25 109 2 washing wash VBG cord-013178-li1x1m25 109 3 three three CD cord-013178-li1x1m25 109 4 times time NNS cord-013178-li1x1m25 109 5 with with IN cord-013178-li1x1m25 109 6 PBS PBS NNP cord-013178-li1x1m25 109 7 , , , cord-013178-li1x1m25 109 8 the the DT cord-013178-li1x1m25 109 9 cell cell NN cord-013178-li1x1m25 109 10 pellet pellet NN cord-013178-li1x1m25 109 11 was be VBD cord-013178-li1x1m25 109 12 resuspended resuspend VBN cord-013178-li1x1m25 109 13 in in IN cord-013178-li1x1m25 109 14 0.5 0.5 CD cord-013178-li1x1m25 109 15 mL ml NN cord-013178-li1x1m25 109 16 of of IN cord-013178-li1x1m25 109 17 fetal fetal JJ cord-013178-li1x1m25 109 18 bovine bovine JJ cord-013178-li1x1m25 109 19 serum serum NN cord-013178-li1x1m25 109 20 . . . cord-013178-li1x1m25 110 1 An an DT cord-013178-li1x1m25 110 2 aliquot aliquot NN cord-013178-li1x1m25 110 3 ( ( -LRB- cord-013178-li1x1m25 110 4 25 25 CD cord-013178-li1x1m25 110 5 µL µL NNP cord-013178-li1x1m25 110 6 ) ) -RRB- cord-013178-li1x1m25 110 7 of of IN cord-013178-li1x1m25 110 8 cell cell NN cord-013178-li1x1m25 110 9 suspension suspension NN cord-013178-li1x1m25 110 10 was be VBD cord-013178-li1x1m25 110 11 loaded load VBN cord-013178-li1x1m25 110 12 onto onto IN cord-013178-li1x1m25 110 13 a a DT cord-013178-li1x1m25 110 14 glass glass NN cord-013178-li1x1m25 110 15 slide slide NN cord-013178-li1x1m25 110 16 for for IN cord-013178-li1x1m25 110 17 the the DT cord-013178-li1x1m25 110 18 cell cell NN cord-013178-li1x1m25 110 19 smear smear NN cord-013178-li1x1m25 110 20 . . . cord-013178-li1x1m25 111 1 After after IN cord-013178-li1x1m25 111 2 the the DT cord-013178-li1x1m25 111 3 cell cell NN cord-013178-li1x1m25 111 4 smear smear NN cord-013178-li1x1m25 111 5 , , , cord-013178-li1x1m25 111 6 the the DT cord-013178-li1x1m25 111 7 slides slide NNS cord-013178-li1x1m25 111 8 were be VBD cord-013178-li1x1m25 111 9 air air NN cord-013178-li1x1m25 111 10 - - HYPH cord-013178-li1x1m25 111 11 dried dry VBN cord-013178-li1x1m25 111 12 at at IN cord-013178-li1x1m25 111 13 room room NN cord-013178-li1x1m25 111 14 temperature temperature NN cord-013178-li1x1m25 111 15 . . . cord-013178-li1x1m25 112 1 Thereby thereby RB cord-013178-li1x1m25 112 2 , , , cord-013178-li1x1m25 112 3 the the DT cord-013178-li1x1m25 112 4 PBMCs pbmc NNS cord-013178-li1x1m25 112 5 were be VBD cord-013178-li1x1m25 112 6 stuck stick VBN cord-013178-li1x1m25 112 7 on on IN cord-013178-li1x1m25 112 8 slides slide NNS cord-013178-li1x1m25 112 9 . . . cord-013178-li1x1m25 113 1 These these DT cord-013178-li1x1m25 113 2 slides slide NNS cord-013178-li1x1m25 113 3 were be VBD cord-013178-li1x1m25 113 4 fixed fix VBN cord-013178-li1x1m25 113 5 with with IN cord-013178-li1x1m25 113 6 PBS PBS NNP cord-013178-li1x1m25 113 7 containing contain VBG cord-013178-li1x1m25 113 8 2 2 CD cord-013178-li1x1m25 113 9 % % NN cord-013178-li1x1m25 113 10 paraformaldehyde paraformaldehyde NNP cord-013178-li1x1m25 113 11 at at IN cord-013178-li1x1m25 113 12 4 4 CD cord-013178-li1x1m25 113 13 • • NN cord-013178-li1x1m25 113 14 C C NNP cord-013178-li1x1m25 113 15 for for IN cord-013178-li1x1m25 113 16 10 10 CD cord-013178-li1x1m25 113 17 min min NN cord-013178-li1x1m25 113 18 and and CC cord-013178-li1x1m25 113 19 then then RB cord-013178-li1x1m25 113 20 washed wash VBD cord-013178-li1x1m25 113 21 three three CD cord-013178-li1x1m25 113 22 times time NNS cord-013178-li1x1m25 113 23 with with IN cord-013178-li1x1m25 113 24 PBS PBS NNP cord-013178-li1x1m25 113 25 . . . cord-013178-li1x1m25 114 1 Following follow VBG cord-013178-li1x1m25 114 2 that that IN cord-013178-li1x1m25 114 3 , , , cord-013178-li1x1m25 114 4 the the DT cord-013178-li1x1m25 114 5 slides slide NNS cord-013178-li1x1m25 114 6 were be VBD cord-013178-li1x1m25 114 7 soaked soak VBN cord-013178-li1x1m25 114 8 in in IN cord-013178-li1x1m25 114 9 PBS PBS NNP cord-013178-li1x1m25 114 10 containing contain VBG cord-013178-li1x1m25 115 1 0.1 0.1 CD cord-013178-li1x1m25 115 2 % % NN cord-013178-li1x1m25 115 3 Triton Triton NNP cord-013178-li1x1m25 115 4 X-100 x-100 RB cord-013178-li1x1m25 115 5 at at IN cord-013178-li1x1m25 115 6 4 4 CD cord-013178-li1x1m25 115 7 • • NN cord-013178-li1x1m25 116 1 C C NNP cord-013178-li1x1m25 116 2 for for IN cord-013178-li1x1m25 116 3 3 3 CD cord-013178-li1x1m25 116 4 min min NN cord-013178-li1x1m25 116 5 . . . cord-013178-li1x1m25 117 1 Subsequently subsequently RB cord-013178-li1x1m25 117 2 , , , cord-013178-li1x1m25 117 3 slides slide NNS cord-013178-li1x1m25 117 4 were be VBD cord-013178-li1x1m25 117 5 washed wash VBN cord-013178-li1x1m25 117 6 with with IN cord-013178-li1x1m25 117 7 PBS PBS NNP cord-013178-li1x1m25 117 8 and and CC cord-013178-li1x1m25 117 9 prepared prepare VBN cord-013178-li1x1m25 117 10 for for IN cord-013178-li1x1m25 117 11 IFA IFA NNP cord-013178-li1x1m25 117 12 . . . cord-013178-li1x1m25 118 1 Two two CD cord-013178-li1x1m25 118 2 anti anti JJ cord-013178-li1x1m25 118 3 - - JJ cord-013178-li1x1m25 118 4 capsid capsid JJ cord-013178-li1x1m25 118 5 ( ( -LRB- cord-013178-li1x1m25 118 6 the the DT cord-013178-li1x1m25 118 7 peptide peptide NN cord-013178-li1x1m25 118 8 C3 c3 NN cord-013178-li1x1m25 118 9 ) ) -RRB- cord-013178-li1x1m25 118 10 mAbs mAbs NNP cord-013178-li1x1m25 118 11 ( ( -LRB- cord-013178-li1x1m25 118 12 1H3 1h3 CD cord-013178-li1x1m25 118 13 and and CC cord-013178-li1x1m25 118 14 6B8 6b8 LS cord-013178-li1x1m25 118 15 ) ) -RRB- cord-013178-li1x1m25 118 16 [ [ -LRB- cord-013178-li1x1m25 118 17 49 49 CD cord-013178-li1x1m25 118 18 ] ] -RRB- cord-013178-li1x1m25 118 19 , , , cord-013178-li1x1m25 118 20 one one CD cord-013178-li1x1m25 118 21 anti anti JJ cord-013178-li1x1m25 118 22 - - JJ cord-013178-li1x1m25 118 23 PCV2 pcv2 JJ cord-013178-li1x1m25 118 24 mAb mab NN cord-013178-li1x1m25 118 25 36A9 36a9 XX cord-013178-li1x1m25 118 26 ( ( -LRB- cord-013178-li1x1m25 118 27 Ingenasa Ingenasa NNP cord-013178-li1x1m25 118 28 , , , cord-013178-li1x1m25 118 29 Madrid Madrid NNP cord-013178-li1x1m25 118 30 , , , cord-013178-li1x1m25 118 31 Spain Spain NNP cord-013178-li1x1m25 118 32 ) ) -RRB- cord-013178-li1x1m25 118 33 , , , cord-013178-li1x1m25 118 34 one one CD cord-013178-li1x1m25 118 35 anti anti JJ cord-013178-li1x1m25 118 36 - - JJ cord-013178-li1x1m25 118 37 p53 p53 JJ cord-013178-li1x1m25 118 38 protein protein NN cord-013178-li1x1m25 118 39 ( ( -LRB- cord-013178-li1x1m25 118 40 wt wt NN cord-013178-li1x1m25 118 41 - - HYPH cord-013178-li1x1m25 118 42 p53 p53 CD cord-013178-li1x1m25 118 43 ) ) -RRB- cord-013178-li1x1m25 118 44 rabbit rabbit NN cord-013178-li1x1m25 118 45 polyclonal polyclonal JJ cord-013178-li1x1m25 118 46 antibody antibody NN cord-013178-li1x1m25 118 47 ( ( -LRB- cord-013178-li1x1m25 118 48 Bioss Bioss NNP cord-013178-li1x1m25 118 49 , , , cord-013178-li1x1m25 118 50 Woburn Woburn NNP cord-013178-li1x1m25 118 51 , , , cord-013178-li1x1m25 118 52 MA MA NNP cord-013178-li1x1m25 118 53 , , , cord-013178-li1x1m25 118 54 USA USA NNP cord-013178-li1x1m25 118 55 ) ) -RRB- cord-013178-li1x1m25 118 56 , , , cord-013178-li1x1m25 118 57 the the DT cord-013178-li1x1m25 118 58 homemade homemade JJ cord-013178-li1x1m25 118 59 anti anti NNP cord-013178-li1x1m25 118 60 - - NNP cord-013178-li1x1m25 118 61 ORF3 ORF3 NNP cord-013178-li1x1m25 118 62 peptide peptide NN cord-013178-li1x1m25 118 63 ( ( -LRB- cord-013178-li1x1m25 118 64 N1 N1 NNP cord-013178-li1x1m25 118 65 ) ) -RRB- cord-013178-li1x1m25 118 66 mAb mAb . cord-013178-li1x1m25 118 67 ( ( -LRB- cord-013178-li1x1m25 118 68 7D3 7d3 CD cord-013178-li1x1m25 118 69 ) ) -RRB- cord-013178-li1x1m25 118 70 , , , cord-013178-li1x1m25 118 71 and and CC cord-013178-li1x1m25 118 72 rabbit rabbit NN cord-013178-li1x1m25 118 73 antisera antisera NNP cord-013178-li1x1m25 118 74 ( ( -LRB- cord-013178-li1x1m25 118 75 as as IN cord-013178-li1x1m25 118 76 the the DT cord-013178-li1x1m25 118 77 method method NN cord-013178-li1x1m25 118 78 mentioned mention VBN cord-013178-li1x1m25 118 79 above above RB cord-013178-li1x1m25 118 80 ) ) -RRB- cord-013178-li1x1m25 118 81 were be VBD cord-013178-li1x1m25 118 82 used use VBN cord-013178-li1x1m25 118 83 in in IN cord-013178-li1x1m25 118 84 this this DT cord-013178-li1x1m25 118 85 study study NN cord-013178-li1x1m25 118 86 . . . cord-013178-li1x1m25 119 1 The the DT cord-013178-li1x1m25 119 2 anti anti JJ cord-013178-li1x1m25 119 3 - - JJ cord-013178-li1x1m25 119 4 capsid capsid JJ cord-013178-li1x1m25 119 5 peptide peptide JJ cord-013178-li1x1m25 119 6 mAbs mAbs NNP cord-013178-li1x1m25 119 7 ( ( -LRB- cord-013178-li1x1m25 119 8 1H3 1h3 CD cord-013178-li1x1m25 119 9 and and CC cord-013178-li1x1m25 119 10 6B8 6b8 LS cord-013178-li1x1m25 119 11 ) ) -RRB- cord-013178-li1x1m25 119 12 recognized recognize VBD cord-013178-li1x1m25 119 13 native native JJ cord-013178-li1x1m25 119 14 capsid capsid NN cord-013178-li1x1m25 119 15 proteins protein NNS cord-013178-li1x1m25 119 16 and and CC cord-013178-li1x1m25 119 17 reacted react VBD cord-013178-li1x1m25 119 18 with with IN cord-013178-li1x1m25 119 19 core core NN cord-013178-li1x1m25 119 20 peptides peptide NNS cord-013178-li1x1m25 119 21 ( ( -LRB- cord-013178-li1x1m25 119 22 P59 P59 NNP cord-013178-li1x1m25 119 23 , , , cord-013178-li1x1m25 119 24 227 227 CD cord-013178-li1x1m25 119 25 KDPPLNP KDPPLNP NNP cord-013178-li1x1m25 119 26 233 233 CD cord-013178-li1x1m25 119 27 ) ) -RRB- cord-013178-li1x1m25 119 28 ; ; : cord-013178-li1x1m25 119 29 however however RB cord-013178-li1x1m25 119 30 , , , cord-013178-li1x1m25 119 31 the the DT cord-013178-li1x1m25 119 32 mAb mAb NNP cord-013178-li1x1m25 119 33 1H3 1H3 NNP cord-013178-li1x1m25 119 34 bound bind VBD cord-013178-li1x1m25 119 35 the the DT cord-013178-li1x1m25 119 36 three three CD cord-013178-li1x1m25 119 37 minimal minimal JJ cord-013178-li1x1m25 119 38 linear linear JJ cord-013178-li1x1m25 119 39 epitopes epitope NNS cord-013178-li1x1m25 119 40 ( ( -LRB- cord-013178-li1x1m25 119 41 DPPLNP DPPLNP NNP cord-013178-li1x1m25 119 42 , , , cord-013178-li1x1m25 119 43 DPPLNPK DPPLNPK NNP cord-013178-li1x1m25 119 44 , , , cord-013178-li1x1m25 119 45 and and CC cord-013178-li1x1m25 119 46 LKDPPLKP LKDPPLKP NNP cord-013178-li1x1m25 119 47 ) ) -RRB- cord-013178-li1x1m25 119 48 and and CC cord-013178-li1x1m25 119 49 the the DT cord-013178-li1x1m25 119 50 mAb mAb NNS cord-013178-li1x1m25 119 51 6B8 6b8 RB cord-013178-li1x1m25 119 52 bound bind VBD cord-013178-li1x1m25 119 53 two two CD cord-013178-li1x1m25 119 54 minimal minimal JJ cord-013178-li1x1m25 119 55 linear linear JJ cord-013178-li1x1m25 119 56 epitopes epitope NNS cord-013178-li1x1m25 119 57 ( ( -LRB- cord-013178-li1x1m25 119 58 KDPPLNP KDPPLNP NNP cord-013178-li1x1m25 119 59 and and CC cord-013178-li1x1m25 119 60 KDPPLNPK KDPPLNPK NNP cord-013178-li1x1m25 119 61 ) ) -RRB- cord-013178-li1x1m25 119 62 . . . cord-013178-li1x1m25 120 1 These these DT cord-013178-li1x1m25 120 2 mAbs mabs NN cord-013178-li1x1m25 120 3 produced produce VBD cord-013178-li1x1m25 120 4 a a DT cord-013178-li1x1m25 120 5 variable variable JJ cord-013178-li1x1m25 120 6 definite definite JJ cord-013178-li1x1m25 120 7 staining staining NN cord-013178-li1x1m25 120 8 pattern pattern NN cord-013178-li1x1m25 120 9 in in IN cord-013178-li1x1m25 120 10 PBMCs pbmc NNS cord-013178-li1x1m25 120 11 by by IN cord-013178-li1x1m25 120 12 IFA IFA NNP cord-013178-li1x1m25 120 13 [ [ -LRB- cord-013178-li1x1m25 120 14 49 49 CD cord-013178-li1x1m25 120 15 ] ] -RRB- cord-013178-li1x1m25 120 16 . . . cord-013178-li1x1m25 121 1 The the DT cord-013178-li1x1m25 121 2 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 121 3 PBMCs pbmc NNS cord-013178-li1x1m25 121 4 slides slide NNS cord-013178-li1x1m25 121 5 were be VBD cord-013178-li1x1m25 121 6 incubated incubate VBN cord-013178-li1x1m25 121 7 with with IN cord-013178-li1x1m25 121 8 a a DT cord-013178-li1x1m25 121 9 1:100 1:100 CD cord-013178-li1x1m25 121 10 dilution dilution NN cord-013178-li1x1m25 121 11 of of IN cord-013178-li1x1m25 121 12 rabbit rabbit NN cord-013178-li1x1m25 121 13 antiserum antiserum NN cord-013178-li1x1m25 121 14 and and CC cord-013178-li1x1m25 121 15 a a DT cord-013178-li1x1m25 121 16 1:100 1:100 CD cord-013178-li1x1m25 121 17 dilution dilution NN cord-013178-li1x1m25 121 18 of of IN cord-013178-li1x1m25 121 19 mAb mab NN cord-013178-li1x1m25 121 20 ( ( -LRB- cord-013178-li1x1m25 121 21 ascites ascites NN cord-013178-li1x1m25 121 22 ) ) -RRB- cord-013178-li1x1m25 121 23 . . . cord-013178-li1x1m25 122 1 After after IN cord-013178-li1x1m25 122 2 incubation incubation NN cord-013178-li1x1m25 122 3 at at IN cord-013178-li1x1m25 122 4 37 37 CD cord-013178-li1x1m25 122 5 • • NN cord-013178-li1x1m25 122 6 C C NNP cord-013178-li1x1m25 122 7 for for IN cord-013178-li1x1m25 122 8 1 1 CD cord-013178-li1x1m25 122 9 h h NN cord-013178-li1x1m25 122 10 , , , cord-013178-li1x1m25 122 11 the the DT cord-013178-li1x1m25 122 12 slides slide NNS cord-013178-li1x1m25 122 13 were be VBD cord-013178-li1x1m25 122 14 gently gently RB cord-013178-li1x1m25 122 15 rinsed rinse VBN cord-013178-li1x1m25 122 16 briefly briefly NN cord-013178-li1x1m25 122 17 in in IN cord-013178-li1x1m25 122 18 PBS PBS NNP cord-013178-li1x1m25 122 19 and and CC cord-013178-li1x1m25 122 20 then then RB cord-013178-li1x1m25 122 21 soaked soak VBD cord-013178-li1x1m25 122 22 for for IN cord-013178-li1x1m25 122 23 15 15 CD cord-013178-li1x1m25 122 24 min min NN cord-013178-li1x1m25 122 25 in in IN cord-013178-li1x1m25 122 26 PBS PBS NNP cord-013178-li1x1m25 122 27 at at IN cord-013178-li1x1m25 122 28 4 4 CD cord-013178-li1x1m25 122 29 • • NN cord-013178-li1x1m25 122 30 C. c. NN cord-013178-li1x1m25 123 1 The the DT cord-013178-li1x1m25 123 2 slides slide NNS cord-013178-li1x1m25 123 3 were be VBD cord-013178-li1x1m25 123 4 then then RB cord-013178-li1x1m25 123 5 incubated incubate VBN cord-013178-li1x1m25 123 6 at at IN cord-013178-li1x1m25 123 7 37 37 CD cord-013178-li1x1m25 123 8 • • NN cord-013178-li1x1m25 123 9 C c NN cord-013178-li1x1m25 123 10 with with IN cord-013178-li1x1m25 123 11 TRITC tritc NN cord-013178-li1x1m25 123 12 - - HYPH cord-013178-li1x1m25 123 13 conjugated conjugate VBN cord-013178-li1x1m25 123 14 goat goat NN cord-013178-li1x1m25 123 15 anti anti JJ cord-013178-li1x1m25 123 16 - - JJ cord-013178-li1x1m25 123 17 mouse mouse JJ cord-013178-li1x1m25 123 18 IgG IgG NNP cord-013178-li1x1m25 123 19 ( ( -LRB- cord-013178-li1x1m25 123 20 subclasses subclass NNS cord-013178-li1x1m25 123 21 1 1 CD cord-013178-li1x1m25 123 22 + + SYM cord-013178-li1x1m25 123 23 2a 2a CD cord-013178-li1x1m25 123 24 + + SYM cord-013178-li1x1m25 123 25 2b 2b CD cord-013178-li1x1m25 124 1 + + SYM cord-013178-li1x1m25 124 2 3 3 CD cord-013178-li1x1m25 124 3 , , , cord-013178-li1x1m25 124 4 Fcγ Fcγ NNP cord-013178-li1x1m25 124 5 ) ) -RRB- cord-013178-li1x1m25 124 6 at at IN cord-013178-li1x1m25 124 7 a a DT cord-013178-li1x1m25 124 8 1:100 1:100 CD cord-013178-li1x1m25 124 9 dilution dilution NN cord-013178-li1x1m25 124 10 and and CC cord-013178-li1x1m25 124 11 FITC FITC NNP cord-013178-li1x1m25 124 12 - - HYPH cord-013178-li1x1m25 124 13 conjugated conjugate VBN cord-013178-li1x1m25 124 14 goat goat NN cord-013178-li1x1m25 124 15 anti anti JJ cord-013178-li1x1m25 124 16 - - JJ cord-013178-li1x1m25 124 17 rabbit rabbit JJ cord-013178-li1x1m25 124 18 IgG igg NN cord-013178-li1x1m25 124 19 ( ( -LRB- cord-013178-li1x1m25 124 20 H H NNP cord-013178-li1x1m25 124 21 + + CC cord-013178-li1x1m25 124 22 L l NN cord-013178-li1x1m25 124 23 ) ) -RRB- cord-013178-li1x1m25 125 1 ( ( -LRB- cord-013178-li1x1m25 125 2 all all DT cord-013178-li1x1m25 125 3 from from IN cord-013178-li1x1m25 125 4 Jackson Jackson NNP cord-013178-li1x1m25 125 5 ImmunoResearch ImmunoResearch NNP cord-013178-li1x1m25 125 6 , , , cord-013178-li1x1m25 125 7 West West NNP cord-013178-li1x1m25 125 8 Grove Grove NNP cord-013178-li1x1m25 125 9 , , , cord-013178-li1x1m25 125 10 PA PA NNP cord-013178-li1x1m25 125 11 , , , cord-013178-li1x1m25 125 12 USA USA NNP cord-013178-li1x1m25 125 13 , , , cord-013178-li1x1m25 125 14 and and CC cord-013178-li1x1m25 125 15 each each DT cord-013178-li1x1m25 125 16 minimal minimal JJ cord-013178-li1x1m25 125 17 antibody antibody NN cord-013178-li1x1m25 125 18 cross cross NN cord-013178-li1x1m25 125 19 - - NN cord-013178-li1x1m25 125 20 reaction reaction NN cord-013178-li1x1m25 125 21 to to IN cord-013178-li1x1m25 125 22 other other JJ cord-013178-li1x1m25 125 23 animal animal NN cord-013178-li1x1m25 125 24 's 's POS cord-013178-li1x1m25 125 25 serum serum NN cord-013178-li1x1m25 125 26 protein protein NN cord-013178-li1x1m25 125 27 ) ) -RRB- cord-013178-li1x1m25 125 28 at at IN cord-013178-li1x1m25 125 29 a a DT cord-013178-li1x1m25 125 30 1:100 1:100 CD cord-013178-li1x1m25 125 31 dilution dilution NN cord-013178-li1x1m25 125 32 . . . cord-013178-li1x1m25 126 1 After after IN cord-013178-li1x1m25 126 2 30 30 CD cord-013178-li1x1m25 126 3 min min NN cord-013178-li1x1m25 126 4 of of IN cord-013178-li1x1m25 126 5 incubation incubation NN cord-013178-li1x1m25 126 6 , , , cord-013178-li1x1m25 126 7 the the DT cord-013178-li1x1m25 126 8 slides slide NNS cord-013178-li1x1m25 126 9 were be VBD cord-013178-li1x1m25 126 10 washed wash VBN cord-013178-li1x1m25 126 11 with with IN cord-013178-li1x1m25 126 12 PBS PBS NNP cord-013178-li1x1m25 126 13 and and CC cord-013178-li1x1m25 126 14 then then RB cord-013178-li1x1m25 126 15 incubated incubate VBD cord-013178-li1x1m25 126 16 with with IN cord-013178-li1x1m25 126 17 4 4 CD cord-013178-li1x1m25 126 18 , , , cord-013178-li1x1m25 126 19 6-Diamidino-2-phenylindole 6-diamidino-2-phenylindole CD cord-013178-li1x1m25 126 20 , , , cord-013178-li1x1m25 126 21 dihydrochloride dihydrochloride NNP cord-013178-li1x1m25 126 22 ( ( -LRB- cord-013178-li1x1m25 126 23 DAPI dapi XX cord-013178-li1x1m25 126 24 , , , cord-013178-li1x1m25 126 25 AAT AAT NNP cord-013178-li1x1m25 126 26 Bioquest Bioquest NNP cord-013178-li1x1m25 126 27 , , , cord-013178-li1x1m25 126 28 Sunnyvale Sunnyvale NNP cord-013178-li1x1m25 126 29 , , , cord-013178-li1x1m25 126 30 CA CA NNP cord-013178-li1x1m25 126 31 , , , cord-013178-li1x1m25 126 32 USA USA NNP cord-013178-li1x1m25 126 33 ) ) -RRB- cord-013178-li1x1m25 126 34 at at IN cord-013178-li1x1m25 126 35 a a DT cord-013178-li1x1m25 126 36 1:2300 1:2300 CD cord-013178-li1x1m25 126 37 dilution dilution NN cord-013178-li1x1m25 126 38 in in IN cord-013178-li1x1m25 126 39 PBS PBS NNP cord-013178-li1x1m25 126 40 at at IN cord-013178-li1x1m25 126 41 room room NN cord-013178-li1x1m25 126 42 temperature temperature NN cord-013178-li1x1m25 126 43 for for IN cord-013178-li1x1m25 126 44 15 15 CD cord-013178-li1x1m25 126 45 min min NN cord-013178-li1x1m25 126 46 . . . cord-013178-li1x1m25 127 1 The the DT cord-013178-li1x1m25 127 2 slides slide NNS cord-013178-li1x1m25 127 3 were be VBD cord-013178-li1x1m25 127 4 mounted mount VBN cord-013178-li1x1m25 127 5 under under IN cord-013178-li1x1m25 127 6 50 50 CD cord-013178-li1x1m25 127 7 % % NN cord-013178-li1x1m25 127 8 glycerol glycerol NN cord-013178-li1x1m25 127 9 and and CC cord-013178-li1x1m25 127 10 observed observe VBD cord-013178-li1x1m25 127 11 with with IN cord-013178-li1x1m25 127 12 an an DT cord-013178-li1x1m25 127 13 Olympus Olympus NNP cord-013178-li1x1m25 127 14 BX51 BX51 NNP cord-013178-li1x1m25 127 15 fluorescence fluorescence NN cord-013178-li1x1m25 127 16 microscope microscope NN cord-013178-li1x1m25 127 17 and and CC cord-013178-li1x1m25 127 18 SPOT SPOT NNP cord-013178-li1x1m25 127 19 Flex flex JJ cord-013178-li1x1m25 127 20 camera camera NN cord-013178-li1x1m25 127 21 ( ( -LRB- cord-013178-li1x1m25 127 22 Diagnostic Diagnostic NNP cord-013178-li1x1m25 127 23 Instrument Instrument NNP cord-013178-li1x1m25 127 24 , , , cord-013178-li1x1m25 127 25 Model Model NNP cord-013178-li1x1m25 127 26 15.2 15.2 CD cord-013178-li1x1m25 127 27 64MP 64mp CD cord-013178-li1x1m25 127 28 , , , cord-013178-li1x1m25 127 29 USA USA NNP cord-013178-li1x1m25 127 30 ) ) -RRB- cord-013178-li1x1m25 127 31 . . . cord-013178-li1x1m25 128 1 Terminal terminal JJ cord-013178-li1x1m25 128 2 deoxynucleotidyl deoxynucleotidyl NNP cord-013178-li1x1m25 128 3 transferase transferase NNP cord-013178-li1x1m25 128 4 dUTP dUTP NNP cord-013178-li1x1m25 128 5 nick nick NNP cord-013178-li1x1m25 128 6 end end NNP cord-013178-li1x1m25 128 7 labeling label VBG cord-013178-li1x1m25 128 8 ( ( -LRB- cord-013178-li1x1m25 128 9 TUNEL TUNEL NNP cord-013178-li1x1m25 128 10 ) ) -RRB- cord-013178-li1x1m25 128 11 assay assay NN cord-013178-li1x1m25 128 12 was be VBD cord-013178-li1x1m25 128 13 performed perform VBN cord-013178-li1x1m25 128 14 according accord VBG cord-013178-li1x1m25 128 15 to to IN cord-013178-li1x1m25 128 16 the the DT cord-013178-li1x1m25 128 17 manufacturer manufacturer NN cord-013178-li1x1m25 128 18 's 's POS cord-013178-li1x1m25 128 19 instructions instruction NNS cord-013178-li1x1m25 128 20 ( ( -LRB- cord-013178-li1x1m25 128 21 The the DT cord-013178-li1x1m25 128 22 Cell Cell NNP cord-013178-li1x1m25 128 23 Meter Meter NNP cord-013178-li1x1m25 128 24 ™ ™ NN cord-013178-li1x1m25 128 25 TUNEL TUNEL NNP cord-013178-li1x1m25 128 26 Apoptosis Apoptosis NNP cord-013178-li1x1m25 128 27 Assay Assay NNP cord-013178-li1x1m25 128 28 kit kit NN cord-013178-li1x1m25 128 29 , , , cord-013178-li1x1m25 128 30 AAT AAT NNP cord-013178-li1x1m25 128 31 Bioquest Bioquest NNP cord-013178-li1x1m25 128 32 ® ® NNP cord-013178-li1x1m25 128 33 , , , cord-013178-li1x1m25 128 34 Inc. Inc. NNP cord-013178-li1x1m25 128 35 , , , cord-013178-li1x1m25 128 36 Sunnyvale Sunnyvale NNP cord-013178-li1x1m25 128 37 , , , cord-013178-li1x1m25 128 38 CA CA NNP cord-013178-li1x1m25 128 39 , , , cord-013178-li1x1m25 128 40 USA USA NNP cord-013178-li1x1m25 128 41 ) ) -RRB- cord-013178-li1x1m25 128 42 to to TO cord-013178-li1x1m25 128 43 detect detect VB cord-013178-li1x1m25 128 44 excessive excessive JJ cord-013178-li1x1m25 128 45 DNA dna NN cord-013178-li1x1m25 128 46 breakage breakage NN cord-013178-li1x1m25 128 47 in in IN cord-013178-li1x1m25 128 48 the the DT cord-013178-li1x1m25 128 49 PBMC PBMC NNP cord-013178-li1x1m25 128 50 . . . cord-013178-li1x1m25 129 1 Briefly briefly RB cord-013178-li1x1m25 129 2 , , , cord-013178-li1x1m25 129 3 the the DT cord-013178-li1x1m25 129 4 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 129 5 PBMCs pbmcs JJ cord-013178-li1x1m25 129 6 slides slide NNS cord-013178-li1x1m25 129 7 were be VBD cord-013178-li1x1m25 129 8 incubated incubate VBN cord-013178-li1x1m25 129 9 with with IN cord-013178-li1x1m25 129 10 the the DT cord-013178-li1x1m25 129 11 TF3-dUTP TF3-dUTP NNP cord-013178-li1x1m25 129 12 / / SYM cord-013178-li1x1m25 129 13 reaction reaction NN cord-013178-li1x1m25 129 14 buffer buffer NN cord-013178-li1x1m25 129 15 . . . cord-013178-li1x1m25 130 1 After after IN cord-013178-li1x1m25 130 2 60 60 CD cord-013178-li1x1m25 130 3 min min NN cord-013178-li1x1m25 130 4 of of IN cord-013178-li1x1m25 130 5 incubation incubation NN cord-013178-li1x1m25 130 6 , , , cord-013178-li1x1m25 130 7 the the DT cord-013178-li1x1m25 130 8 slides slide NNS cord-013178-li1x1m25 130 9 were be VBD cord-013178-li1x1m25 130 10 washed wash VBN cord-013178-li1x1m25 130 11 with with IN cord-013178-li1x1m25 130 12 PBS PBS NNP cord-013178-li1x1m25 130 13 . . . cord-013178-li1x1m25 131 1 They -PRON- PRP cord-013178-li1x1m25 131 2 were be VBD cord-013178-li1x1m25 131 3 then then RB cord-013178-li1x1m25 131 4 incubated incubate VBN cord-013178-li1x1m25 131 5 with with IN cord-013178-li1x1m25 131 6 Hoechst Hoechst NNP cord-013178-li1x1m25 131 7 solution solution NN cord-013178-li1x1m25 131 8 at at IN cord-013178-li1x1m25 131 9 room room NN cord-013178-li1x1m25 131 10 temperature temperature NN cord-013178-li1x1m25 131 11 for for IN cord-013178-li1x1m25 131 12 13 13 CD cord-013178-li1x1m25 131 13 min min NN cord-013178-li1x1m25 131 14 . . . cord-013178-li1x1m25 132 1 The the DT cord-013178-li1x1m25 132 2 slides slide NNS cord-013178-li1x1m25 132 3 were be VBD cord-013178-li1x1m25 132 4 mounted mount VBN cord-013178-li1x1m25 132 5 under under IN cord-013178-li1x1m25 132 6 50 50 CD cord-013178-li1x1m25 132 7 % % NN cord-013178-li1x1m25 132 8 glycerol glycerol NN cord-013178-li1x1m25 132 9 and and CC cord-013178-li1x1m25 132 10 observed observe VBD cord-013178-li1x1m25 132 11 with with IN cord-013178-li1x1m25 132 12 an an DT cord-013178-li1x1m25 132 13 Olympus Olympus NNP cord-013178-li1x1m25 132 14 BX51 BX51 NNP cord-013178-li1x1m25 132 15 fluorescence fluorescence NN cord-013178-li1x1m25 132 16 microscope microscope NN cord-013178-li1x1m25 132 17 and and CC cord-013178-li1x1m25 132 18 SPOT SPOT NNP cord-013178-li1x1m25 132 19 Flex flex JJ cord-013178-li1x1m25 132 20 camera camera NN cord-013178-li1x1m25 132 21 . . . cord-013178-li1x1m25 133 1 Blood blood NN cord-013178-li1x1m25 133 2 samples sample NNS cord-013178-li1x1m25 133 3 were be VBD cord-013178-li1x1m25 133 4 collected collect VBN cord-013178-li1x1m25 133 5 from from IN cord-013178-li1x1m25 133 6 the the DT cord-013178-li1x1m25 133 7 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 133 8 herd herd NN cord-013178-li1x1m25 133 9 with with IN cord-013178-li1x1m25 133 10 the the DT cord-013178-li1x1m25 133 11 following follow VBG cord-013178-li1x1m25 133 12 vaccination vaccination NN cord-013178-li1x1m25 133 13 at at IN cord-013178-li1x1m25 133 14 two two CD cord-013178-li1x1m25 133 15 weeks week NNS cord-013178-li1x1m25 133 16 of of IN cord-013178-li1x1m25 133 17 age age NN cord-013178-li1x1m25 133 18 with with IN cord-013178-li1x1m25 133 19 a a DT cord-013178-li1x1m25 133 20 PCV2 PCV2 NNP cord-013178-li1x1m25 133 21 vaccine vaccine NN cord-013178-li1x1m25 133 22 ( ( -LRB- cord-013178-li1x1m25 133 23 2 2 CD cord-013178-li1x1m25 133 24 mL ml NN cord-013178-li1x1m25 133 25 , , , cord-013178-li1x1m25 133 26 Porcilis Porcilis NNP cord-013178-li1x1m25 133 27 ® ® VBP cord-013178-li1x1m25 133 28 PCV PCV NNP cord-013178-li1x1m25 133 29 , , , cord-013178-li1x1m25 133 30 MSD MSD NNP cord-013178-li1x1m25 133 31 Animal Animal NNP cord-013178-li1x1m25 133 32 Health Health NNP cord-013178-li1x1m25 133 33 ) ) -RRB- cord-013178-li1x1m25 133 34 in in IN cord-013178-li1x1m25 133 35 the the DT cord-013178-li1x1m25 133 36 conventional conventional JJ cord-013178-li1x1m25 133 37 pig pig NN cord-013178-li1x1m25 133 38 farm farm NN cord-013178-li1x1m25 133 39 with with IN cord-013178-li1x1m25 133 40 the the DT cord-013178-li1x1m25 133 41 farrow farrow NN cord-013178-li1x1m25 133 42 - - HYPH cord-013178-li1x1m25 133 43 to to IN cord-013178-li1x1m25 133 44 - - HYPH cord-013178-li1x1m25 133 45 finish finish NN cord-013178-li1x1m25 133 46 operation operation NN cord-013178-li1x1m25 133 47 . . . cord-013178-li1x1m25 134 1 Pigs pig NNS cord-013178-li1x1m25 134 2 were be VBD cord-013178-li1x1m25 134 3 selected select VBN cord-013178-li1x1m25 134 4 from from IN cord-013178-li1x1m25 134 5 animals animal NNS cord-013178-li1x1m25 134 6 of of IN cord-013178-li1x1m25 134 7 different different JJ cord-013178-li1x1m25 134 8 ages age NNS cord-013178-li1x1m25 134 9 ( ( -LRB- cord-013178-li1x1m25 134 10 1 1 CD cord-013178-li1x1m25 134 11 , , , cord-013178-li1x1m25 134 12 3 3 CD cord-013178-li1x1m25 134 13 , , , cord-013178-li1x1m25 134 14 6 6 CD cord-013178-li1x1m25 134 15 , , , cord-013178-li1x1m25 134 16 9 9 CD cord-013178-li1x1m25 134 17 , , , cord-013178-li1x1m25 134 18 12 12 CD cord-013178-li1x1m25 134 19 , , , cord-013178-li1x1m25 134 20 15 15 CD cord-013178-li1x1m25 134 21 , , , cord-013178-li1x1m25 134 22 18 18 CD cord-013178-li1x1m25 134 23 , , , cord-013178-li1x1m25 134 24 21 21 CD cord-013178-li1x1m25 134 25 , , , cord-013178-li1x1m25 134 26 24 24 CD cord-013178-li1x1m25 134 27 , , , cord-013178-li1x1m25 134 28 and and CC cord-013178-li1x1m25 134 29 ≥34 ≥34 NNP cord-013178-li1x1m25 134 30 weeks week NNS cord-013178-li1x1m25 134 31 of of IN cord-013178-li1x1m25 134 32 age age NN cord-013178-li1x1m25 134 33 ) ) -RRB- cord-013178-li1x1m25 134 34 at at IN cord-013178-li1x1m25 134 35 one one CD cord-013178-li1x1m25 134 36 time time NN cord-013178-li1x1m25 134 37 . . . cord-013178-li1x1m25 135 1 Then then RB cord-013178-li1x1m25 135 2 , , , cord-013178-li1x1m25 135 3 four four CD cord-013178-li1x1m25 135 4 samples sample NNS cord-013178-li1x1m25 135 5 were be VBD cord-013178-li1x1m25 135 6 randomly randomly RB cord-013178-li1x1m25 135 7 chosen choose VBN cord-013178-li1x1m25 135 8 per per IN cord-013178-li1x1m25 135 9 age age NN cord-013178-li1x1m25 135 10 group group NN cord-013178-li1x1m25 135 11 . . . cord-013178-li1x1m25 136 1 Blood blood NN cord-013178-li1x1m25 136 2 was be VBD cord-013178-li1x1m25 136 3 collected collect VBN cord-013178-li1x1m25 136 4 in in IN cord-013178-li1x1m25 136 5 the the DT cord-013178-li1x1m25 136 6 Vacutainer Vacutainer NNP cord-013178-li1x1m25 136 7 ® ® HYPH cord-013178-li1x1m25 136 8 EDTA EDTA NNP cord-013178-li1x1m25 136 9 tubes tube NNS cord-013178-li1x1m25 136 10 ( ( -LRB- cord-013178-li1x1m25 136 11 Becton Becton NNP cord-013178-li1x1m25 136 12 and and CC cord-013178-li1x1m25 136 13 Dickinson Dickinson NNP cord-013178-li1x1m25 136 14 BV BV NNP cord-013178-li1x1m25 136 15 ) ) -RRB- cord-013178-li1x1m25 136 16 and and CC cord-013178-li1x1m25 136 17 sored sore VBD cord-013178-li1x1m25 136 18 at at IN cord-013178-li1x1m25 136 19 −20 −20 NNP cord-013178-li1x1m25 136 20 • • NNP cord-013178-li1x1m25 136 21 C. C. NNP cord-013178-li1x1m25 137 1 All all DT cord-013178-li1x1m25 137 2 whole whole JJ cord-013178-li1x1m25 137 3 - - HYPH cord-013178-li1x1m25 137 4 blood blood NN cord-013178-li1x1m25 137 5 samples sample NNS cord-013178-li1x1m25 137 6 had have VBD cord-013178-li1x1m25 137 7 been be VBN cord-013178-li1x1m25 137 8 treated treat VBN cord-013178-li1x1m25 137 9 by by IN cord-013178-li1x1m25 137 10 the the DT cord-013178-li1x1m25 137 11 freeze freeze NN cord-013178-li1x1m25 137 12 - - HYPH cord-013178-li1x1m25 137 13 thaw thaw VB cord-013178-li1x1m25 137 14 process process NN cord-013178-li1x1m25 137 15 twice twice RB cord-013178-li1x1m25 137 16 before before IN cord-013178-li1x1m25 137 17 the the DT cord-013178-li1x1m25 137 18 test test NN cord-013178-li1x1m25 137 19 . . . cord-013178-li1x1m25 138 1 The the DT cord-013178-li1x1m25 138 2 capsid capsid JJ cord-013178-li1x1m25 138 3 protein protein NN cord-013178-li1x1m25 138 4 was be VBD cord-013178-li1x1m25 138 5 detected detect VBN cord-013178-li1x1m25 138 6 from from IN cord-013178-li1x1m25 138 7 50 50 CD cord-013178-li1x1m25 138 8 µL µl CD cord-013178-li1x1m25 138 9 of of IN cord-013178-li1x1m25 138 10 whole whole JJ cord-013178-li1x1m25 138 11 - - HYPH cord-013178-li1x1m25 138 12 blood blood NN cord-013178-li1x1m25 138 13 sample sample NN cord-013178-li1x1m25 138 14 by by IN cord-013178-li1x1m25 138 15 using use VBG cord-013178-li1x1m25 138 16 the the DT cord-013178-li1x1m25 138 17 INGEZIM INGEZIM NNP cord-013178-li1x1m25 138 18 PCV PCV NNP cord-013178-li1x1m25 138 19 DAS DAS NNP cord-013178-li1x1m25 138 20 kit kit NN cord-013178-li1x1m25 138 21 ( ( -LRB- cord-013178-li1x1m25 138 22 Ingenasa Ingenasa NNP cord-013178-li1x1m25 138 23 , , , cord-013178-li1x1m25 138 24 Madrid Madrid NNP cord-013178-li1x1m25 138 25 , , , cord-013178-li1x1m25 138 26 Spain Spain NNP cord-013178-li1x1m25 138 27 ) ) -RRB- cord-013178-li1x1m25 138 28 according accord VBG cord-013178-li1x1m25 138 29 to to IN cord-013178-li1x1m25 138 30 the the DT cord-013178-li1x1m25 138 31 manufacturer manufacturer NN cord-013178-li1x1m25 138 32 's 's POS cord-013178-li1x1m25 138 33 instructions instruction NNS cord-013178-li1x1m25 138 34 , , , cord-013178-li1x1m25 138 35 and and CC cord-013178-li1x1m25 138 36 the the DT cord-013178-li1x1m25 138 37 absorbance absorbance NN cord-013178-li1x1m25 138 38 of of IN cord-013178-li1x1m25 138 39 each each DT cord-013178-li1x1m25 138 40 well well NN cord-013178-li1x1m25 138 41 was be VBD cord-013178-li1x1m25 138 42 measured measure VBN cord-013178-li1x1m25 138 43 at at IN cord-013178-li1x1m25 138 44 450 450 CD cord-013178-li1x1m25 138 45 nm nm NN cord-013178-li1x1m25 138 46 . . . cord-013178-li1x1m25 139 1 Meanwhile meanwhile RB cord-013178-li1x1m25 139 2 , , , cord-013178-li1x1m25 139 3 the the DT cord-013178-li1x1m25 139 4 homemade homemade JJ cord-013178-li1x1m25 139 5 antigen antigen NN cord-013178-li1x1m25 139 6 - - HYPH cord-013178-li1x1m25 139 7 ELISA ELISA NNP cord-013178-li1x1m25 139 8 was be VBD cord-013178-li1x1m25 139 9 conducted conduct VBN cord-013178-li1x1m25 139 10 in in IN cord-013178-li1x1m25 139 11 this this DT cord-013178-li1x1m25 139 12 study study NN cord-013178-li1x1m25 139 13 for for IN cord-013178-li1x1m25 139 14 detecting detect VBG cord-013178-li1x1m25 139 15 the the DT cord-013178-li1x1m25 139 16 ORF3 ORF3 NNP cord-013178-li1x1m25 139 17 protein protein NN cord-013178-li1x1m25 139 18 in in IN cord-013178-li1x1m25 139 19 whole whole JJ cord-013178-li1x1m25 139 20 - - HYPH cord-013178-li1x1m25 139 21 blood blood NN cord-013178-li1x1m25 139 22 specimens specimen NNS cord-013178-li1x1m25 139 23 . . . cord-013178-li1x1m25 140 1 It -PRON- PRP cord-013178-li1x1m25 140 2 was be VBD cord-013178-li1x1m25 140 3 based base VBN cord-013178-li1x1m25 140 4 on on IN cord-013178-li1x1m25 140 5 the the DT cord-013178-li1x1m25 140 6 double double JJ cord-013178-li1x1m25 140 7 - - HYPH cord-013178-li1x1m25 140 8 antibody antibody NN cord-013178-li1x1m25 140 9 - - HYPH cord-013178-li1x1m25 140 10 sandwich sandwich NN cord-013178-li1x1m25 140 11 principle principle NN cord-013178-li1x1m25 140 12 . . . cord-013178-li1x1m25 141 1 Ninety ninety CD cord-013178-li1x1m25 141 2 - - HYPH cord-013178-li1x1m25 141 3 six six CD cord-013178-li1x1m25 141 4 - - HYPH cord-013178-li1x1m25 141 5 well well RB cord-013178-li1x1m25 141 6 Maxisorp Maxisorp NNP cord-013178-li1x1m25 141 7 plates plate NNS cord-013178-li1x1m25 141 8 were be VBD cord-013178-li1x1m25 141 9 coated coat VBN cord-013178-li1x1m25 141 10 with with IN cord-013178-li1x1m25 141 11 1 1 CD cord-013178-li1x1m25 141 12 µL µl XX cord-013178-li1x1m25 142 1 /mL /mL . cord-013178-li1x1m25 142 2 ( ( -LRB- cord-013178-li1x1m25 142 3 100 100 CD cord-013178-li1x1m25 142 4 µL µl XX cord-013178-li1x1m25 142 5 per per IN cord-013178-li1x1m25 142 6 well well RB cord-013178-li1x1m25 142 7 ) ) -RRB- cord-013178-li1x1m25 142 8 of of IN cord-013178-li1x1m25 142 9 the the DT cord-013178-li1x1m25 142 10 trapping trap VBG cord-013178-li1x1m25 142 11 sera sera NNP cord-013178-li1x1m25 142 12 ( ( -LRB- cord-013178-li1x1m25 142 13 rabbit rabbit NNP cord-013178-li1x1m25 142 14 anti anti NNP cord-013178-li1x1m25 142 15 - - NNP cord-013178-li1x1m25 142 16 ORF3 ORF3 NNP cord-013178-li1x1m25 142 17 peptide peptide NN cord-013178-li1x1m25 142 18 ) ) -RRB- cord-013178-li1x1m25 142 19 in in IN cord-013178-li1x1m25 142 20 bicarbonate bicarbonate NN cord-013178-li1x1m25 142 21 buffer buffer NN cord-013178-li1x1m25 142 22 . . . cord-013178-li1x1m25 143 1 The the DT cord-013178-li1x1m25 143 2 plates plate NNS cord-013178-li1x1m25 143 3 were be VBD cord-013178-li1x1m25 143 4 incubated incubate VBN cord-013178-li1x1m25 143 5 at at IN cord-013178-li1x1m25 143 6 4 4 CD cord-013178-li1x1m25 143 7 • • NN cord-013178-li1x1m25 143 8 C C NNP cord-013178-li1x1m25 143 9 overnight overnight RB cord-013178-li1x1m25 143 10 and and CC cord-013178-li1x1m25 143 11 washed wash VBD cord-013178-li1x1m25 143 12 three three CD cord-013178-li1x1m25 143 13 times time NNS cord-013178-li1x1m25 143 14 with with IN cord-013178-li1x1m25 143 15 PBST PBST NNP cord-013178-li1x1m25 143 16 . . . cord-013178-li1x1m25 144 1 Then then RB cord-013178-li1x1m25 144 2 , , , cord-013178-li1x1m25 144 3 the the DT cord-013178-li1x1m25 144 4 plates plate NNS cord-013178-li1x1m25 144 5 were be VBD cord-013178-li1x1m25 144 6 blocked block VBN cord-013178-li1x1m25 144 7 with with IN cord-013178-li1x1m25 144 8 Viruses virus NNS cord-013178-li1x1m25 144 9 2020 2020 CD cord-013178-li1x1m25 144 10 , , , cord-013178-li1x1m25 144 11 12 12 CD cord-013178-li1x1m25 144 12 , , , cord-013178-li1x1m25 144 13 961 961 CD cord-013178-li1x1m25 144 14 7 7 CD cord-013178-li1x1m25 144 15 of of IN cord-013178-li1x1m25 144 16 22 22 CD cord-013178-li1x1m25 144 17 the the DT cord-013178-li1x1m25 144 18 blocking block VBG cord-013178-li1x1m25 144 19 buffer buffer NN cord-013178-li1x1m25 144 20 at at IN cord-013178-li1x1m25 144 21 37 37 CD cord-013178-li1x1m25 145 1 • • NNP cord-013178-li1x1m25 145 2 C C NNP cord-013178-li1x1m25 145 3 for for IN cord-013178-li1x1m25 145 4 30 30 CD cord-013178-li1x1m25 145 5 min min NN cord-013178-li1x1m25 145 6 . . . cord-013178-li1x1m25 146 1 After after IN cord-013178-li1x1m25 146 2 the the DT cord-013178-li1x1m25 146 3 plates plate NNS cord-013178-li1x1m25 146 4 were be VBD cord-013178-li1x1m25 146 5 washed wash VBN cord-013178-li1x1m25 146 6 , , , cord-013178-li1x1m25 146 7 50 50 CD cord-013178-li1x1m25 146 8 µL µl CD cord-013178-li1x1m25 146 9 of of IN cord-013178-li1x1m25 146 10 PBST PBST NNP cord-013178-li1x1m25 146 11 and and CC cord-013178-li1x1m25 147 1 50 50 CD cord-013178-li1x1m25 147 2 µL µl CD cord-013178-li1x1m25 147 3 of of IN cord-013178-li1x1m25 147 4 whole whole JJ cord-013178-li1x1m25 147 5 - - HYPH cord-013178-li1x1m25 147 6 blood blood NN cord-013178-li1x1m25 147 7 samples sample NNS cord-013178-li1x1m25 147 8 were be VBD cord-013178-li1x1m25 147 9 mixed mix VBN cord-013178-li1x1m25 147 10 and and CC cord-013178-li1x1m25 147 11 added add VBN cord-013178-li1x1m25 147 12 to to IN cord-013178-li1x1m25 147 13 the the DT cord-013178-li1x1m25 147 14 well well NN cord-013178-li1x1m25 147 15 . . . cord-013178-li1x1m25 148 1 The the DT cord-013178-li1x1m25 148 2 plates plate NNS cord-013178-li1x1m25 148 3 were be VBD cord-013178-li1x1m25 148 4 incubated incubate VBN cord-013178-li1x1m25 148 5 at at IN cord-013178-li1x1m25 148 6 37 37 CD cord-013178-li1x1m25 148 7 • • NN cord-013178-li1x1m25 148 8 C c NN cord-013178-li1x1m25 148 9 for for IN cord-013178-li1x1m25 148 10 2 2 CD cord-013178-li1x1m25 148 11 h h NN cord-013178-li1x1m25 148 12 and and CC cord-013178-li1x1m25 148 13 then then RB cord-013178-li1x1m25 148 14 washed wash VBD cord-013178-li1x1m25 148 15 in in IN cord-013178-li1x1m25 148 16 PBST PBST NNP cord-013178-li1x1m25 148 17 . . . cord-013178-li1x1m25 149 1 Then then RB cord-013178-li1x1m25 149 2 , , , cord-013178-li1x1m25 149 3 100 100 CD cord-013178-li1x1m25 149 4 µL/ µl/ CD cord-013178-li1x1m25 150 1 well well UH cord-013178-li1x1m25 150 2 of of IN cord-013178-li1x1m25 150 3 mAb mAb NNP cord-013178-li1x1m25 150 4 7D3 7d3 CD cord-013178-li1x1m25 150 5 at at IN cord-013178-li1x1m25 151 1 1 1 CD cord-013178-li1x1m25 151 2 µg µg NN cord-013178-li1x1m25 151 3 / / SYM cord-013178-li1x1m25 151 4 mL mL NNP cord-013178-li1x1m25 151 5 were be VBD cord-013178-li1x1m25 151 6 added add VBN cord-013178-li1x1m25 151 7 to to IN cord-013178-li1x1m25 151 8 the the DT cord-013178-li1x1m25 151 9 plates plate NNS cord-013178-li1x1m25 151 10 , , , cord-013178-li1x1m25 151 11 and and CC cord-013178-li1x1m25 151 12 the the DT cord-013178-li1x1m25 151 13 plates plate NNS cord-013178-li1x1m25 151 14 were be VBD cord-013178-li1x1m25 151 15 incubated incubate VBN cord-013178-li1x1m25 151 16 at at IN cord-013178-li1x1m25 151 17 37 37 CD cord-013178-li1x1m25 151 18 • • NN cord-013178-li1x1m25 151 19 C c NN cord-013178-li1x1m25 151 20 for for IN cord-013178-li1x1m25 151 21 1 1 CD cord-013178-li1x1m25 151 22 h. h. NN cord-013178-li1x1m25 152 1 After after IN cord-013178-li1x1m25 152 2 the the DT cord-013178-li1x1m25 152 3 plates plate NNS cord-013178-li1x1m25 152 4 were be VBD cord-013178-li1x1m25 152 5 washed wash VBN cord-013178-li1x1m25 152 6 , , , cord-013178-li1x1m25 152 7 100 100 CD cord-013178-li1x1m25 152 8 µL/ µl/ NN cord-013178-li1x1m25 153 1 well well UH cord-013178-li1x1m25 153 2 of of IN cord-013178-li1x1m25 153 3 peroxidase peroxidase NN cord-013178-li1x1m25 153 4 - - HYPH cord-013178-li1x1m25 153 5 conjugated conjugate VBN cord-013178-li1x1m25 153 6 goat goat NN cord-013178-li1x1m25 153 7 anti anti JJ cord-013178-li1x1m25 153 8 - - JJ cord-013178-li1x1m25 153 9 mouse mouse JJ cord-013178-li1x1m25 153 10 IgG igg NN cord-013178-li1x1m25 153 11 Fcγ Fcγ NNP cord-013178-li1x1m25 153 12 at at IN cord-013178-li1x1m25 153 13 a a DT cord-013178-li1x1m25 153 14 1:2500 1:2500 CD cord-013178-li1x1m25 153 15 dilution dilution NN cord-013178-li1x1m25 153 16 were be VBD cord-013178-li1x1m25 153 17 added add VBN cord-013178-li1x1m25 153 18 , , , cord-013178-li1x1m25 153 19 and and CC cord-013178-li1x1m25 153 20 the the DT cord-013178-li1x1m25 153 21 plates plate NNS cord-013178-li1x1m25 153 22 were be VBD cord-013178-li1x1m25 153 23 incubated incubate VBN cord-013178-li1x1m25 153 24 at at IN cord-013178-li1x1m25 153 25 37 37 CD cord-013178-li1x1m25 153 26 • • NN cord-013178-li1x1m25 153 27 C c NN cord-013178-li1x1m25 153 28 for for IN cord-013178-li1x1m25 153 29 1 1 CD cord-013178-li1x1m25 153 30 h. h. NN cord-013178-li1x1m25 154 1 After after IN cord-013178-li1x1m25 154 2 washing washing NN cord-013178-li1x1m25 154 3 , , , cord-013178-li1x1m25 154 4 ABTS ABTS NNP cord-013178-li1x1m25 154 5 buffer buffer NN cord-013178-li1x1m25 154 6 was be VBD cord-013178-li1x1m25 154 7 added add VBN cord-013178-li1x1m25 154 8 to to IN cord-013178-li1x1m25 154 9 each each DT cord-013178-li1x1m25 154 10 well well RB cord-013178-li1x1m25 154 11 . . . cord-013178-li1x1m25 155 1 Following follow VBG cord-013178-li1x1m25 155 2 a a DT cord-013178-li1x1m25 155 3 30-min 30-min CD cord-013178-li1x1m25 155 4 incubation incubation NN cord-013178-li1x1m25 155 5 , , , cord-013178-li1x1m25 155 6 the the DT cord-013178-li1x1m25 155 7 OD OD NNP cord-013178-li1x1m25 155 8 was be VBD cord-013178-li1x1m25 155 9 measured measure VBN cord-013178-li1x1m25 155 10 at at IN cord-013178-li1x1m25 155 11 405 405 CD cord-013178-li1x1m25 155 12 nm nm NN cord-013178-li1x1m25 155 13 . . . cord-013178-li1x1m25 156 1 2.9 2.9 CD cord-013178-li1x1m25 156 2 . . . cord-013178-li1x1m25 157 1 Detection detection NN cord-013178-li1x1m25 157 2 of of IN cord-013178-li1x1m25 157 3 the the DT cord-013178-li1x1m25 157 4 Specific specific JJ cord-013178-li1x1m25 157 5 Antibodies Antibodies NNP cord-013178-li1x1m25 157 6 Against against IN cord-013178-li1x1m25 157 7 the the DT cord-013178-li1x1m25 157 8 Capsid Capsid NNP cord-013178-li1x1m25 157 9 Protein Protein NNP cord-013178-li1x1m25 157 10 or or CC cord-013178-li1x1m25 157 11 the the DT cord-013178-li1x1m25 157 12 ORF3 ORF3 NNP cord-013178-li1x1m25 157 13 Protein Protein NNP cord-013178-li1x1m25 157 14 in in IN cord-013178-li1x1m25 157 15 Plasm Plasm NNP cord-013178-li1x1m25 157 16 Samples Samples NNPS cord-013178-li1x1m25 157 17 from from IN cord-013178-li1x1m25 157 18 the the DT cord-013178-li1x1m25 157 19 PCV2-Infected PCV2-Infected NNP cord-013178-li1x1m25 157 20 Herd Herd NNP cord-013178-li1x1m25 157 21 with with IN cord-013178-li1x1m25 157 22 a a DT cord-013178-li1x1m25 157 23 PCV2 PCV2 NNP cord-013178-li1x1m25 157 24 Vaccine Vaccine NNP cord-013178-li1x1m25 157 25 Plasm Plasm NNP cord-013178-li1x1m25 157 26 samples sample NNS cord-013178-li1x1m25 157 27 were be VBD cord-013178-li1x1m25 157 28 separated separate VBN cord-013178-li1x1m25 157 29 from from IN cord-013178-li1x1m25 157 30 the the DT cord-013178-li1x1m25 157 31 aforementioned aforementioned JJ cord-013178-li1x1m25 157 32 fresh fresh JJ cord-013178-li1x1m25 157 33 whole whole JJ cord-013178-li1x1m25 157 34 - - HYPH cord-013178-li1x1m25 157 35 blood blood NN cord-013178-li1x1m25 157 36 samples sample NNS cord-013178-li1x1m25 157 37 and and CC cord-013178-li1x1m25 157 38 sored sore VBD cord-013178-li1x1m25 157 39 at−20 at−20 . cord-013178-li1x1m25 158 1 • • NNP cord-013178-li1x1m25 159 1 C. C. NNP cord-013178-li1x1m25 160 1 The the DT cord-013178-li1x1m25 160 2 antibody antibody NN cord-013178-li1x1m25 160 3 titers titer NNS cord-013178-li1x1m25 160 4 were be VBD cord-013178-li1x1m25 160 5 measured measure VBN cord-013178-li1x1m25 160 6 by by IN cord-013178-li1x1m25 160 7 iELISA iELISA NNP cord-013178-li1x1m25 160 8 [ [ -LRB- cord-013178-li1x1m25 160 9 48 48 CD cord-013178-li1x1m25 160 10 ] ] -RRB- cord-013178-li1x1m25 160 11 ( ( -LRB- cord-013178-li1x1m25 160 12 performed perform VBN cord-013178-li1x1m25 160 13 as as IN cord-013178-li1x1m25 160 14 described describe VBN cord-013178-li1x1m25 160 15 previously previously RB cord-013178-li1x1m25 160 16 for for IN cord-013178-li1x1m25 160 17 pig pig NN cord-013178-li1x1m25 160 18 anti anti JJ cord-013178-li1x1m25 160 19 - - JJ cord-013178-li1x1m25 160 20 peptide peptide JJ cord-013178-li1x1m25 160 21 ELISA ELISA NNP cord-013178-li1x1m25 160 22 , , , cord-013178-li1x1m25 160 23 with with IN cord-013178-li1x1m25 160 24 the the DT cord-013178-li1x1m25 160 25 exception exception NN cord-013178-li1x1m25 160 26 that that IN cord-013178-li1x1m25 160 27 the the DT cord-013178-li1x1m25 160 28 capsid capsid NN cord-013178-li1x1m25 160 29 protein protein NN cord-013178-li1x1m25 160 30 ( ( -LRB- cord-013178-li1x1m25 160 31 CircoFLEX CircoFLEX NNP cord-013178-li1x1m25 160 32 ® ® NNP cord-013178-li1x1m25 160 33 , , , cord-013178-li1x1m25 160 34 Boehringer Boehringer NNP cord-013178-li1x1m25 160 35 Ingelheim Ingelheim NNP cord-013178-li1x1m25 160 36 ) ) -RRB- cord-013178-li1x1m25 160 37 or or CC cord-013178-li1x1m25 160 38 peptide peptide JJ cord-013178-li1x1m25 160 39 P126 P126 NNP cord-013178-li1x1m25 160 40 was be VBD cord-013178-li1x1m25 160 41 used use VBN cord-013178-li1x1m25 160 42 as as IN cord-013178-li1x1m25 160 43 the the DT cord-013178-li1x1m25 160 44 coating coating NN cord-013178-li1x1m25 160 45 antigen antigen NN cord-013178-li1x1m25 160 46 , , , cord-013178-li1x1m25 160 47 and and CC cord-013178-li1x1m25 160 48 pig pig NN cord-013178-li1x1m25 160 49 plasm plasm NN cord-013178-li1x1m25 160 50 at at IN cord-013178-li1x1m25 160 51 a a DT cord-013178-li1x1m25 160 52 1:200 1:200 CD cord-013178-li1x1m25 160 53 dilution dilution NN cord-013178-li1x1m25 160 54 was be VBD cord-013178-li1x1m25 160 55 used use VBN cord-013178-li1x1m25 160 56 ) ) -RRB- cord-013178-li1x1m25 160 57 . . . cord-013178-li1x1m25 161 1 The the DT cord-013178-li1x1m25 161 2 subsequent subsequent JJ cord-013178-li1x1m25 161 3 procedures procedure NNS cord-013178-li1x1m25 161 4 of of IN cord-013178-li1x1m25 161 5 blocking blocking NN cord-013178-li1x1m25 161 6 , , , cord-013178-li1x1m25 161 7 pig pig NN cord-013178-li1x1m25 161 8 antibody antibody NN cord-013178-li1x1m25 161 9 , , , cord-013178-li1x1m25 161 10 washing washing NN cord-013178-li1x1m25 161 11 , , , cord-013178-li1x1m25 161 12 secondary secondary JJ cord-013178-li1x1m25 161 13 antibody antibody NN cord-013178-li1x1m25 161 14 , , , cord-013178-li1x1m25 161 15 substrate substrate NN cord-013178-li1x1m25 161 16 , , , cord-013178-li1x1m25 161 17 and and CC cord-013178-li1x1m25 161 18 the the DT cord-013178-li1x1m25 161 19 OD OD NNP cord-013178-li1x1m25 161 20 reading reading NN cord-013178-li1x1m25 161 21 , , , cord-013178-li1x1m25 161 22 were be VBD cord-013178-li1x1m25 161 23 the the DT cord-013178-li1x1m25 161 24 same same JJ cord-013178-li1x1m25 161 25 as as IN cord-013178-li1x1m25 161 26 described describe VBN cord-013178-li1x1m25 161 27 previously previously RB cord-013178-li1x1m25 161 28 . . . cord-013178-li1x1m25 162 1 The the DT cord-013178-li1x1m25 162 2 data datum NNS cord-013178-li1x1m25 162 3 were be VBD cord-013178-li1x1m25 162 4 analyzed analyze VBN cord-013178-li1x1m25 162 5 by by IN cord-013178-li1x1m25 162 6 using use VBG cord-013178-li1x1m25 162 7 one one CD cord-013178-li1x1m25 162 8 - - HYPH cord-013178-li1x1m25 162 9 way way NN cord-013178-li1x1m25 162 10 analysis analysis NN cord-013178-li1x1m25 162 11 of of IN cord-013178-li1x1m25 162 12 variance variance NN cord-013178-li1x1m25 162 13 ( ( -LRB- cord-013178-li1x1m25 162 14 ANOVA ANOVA NNP cord-013178-li1x1m25 162 15 ) ) -RRB- cord-013178-li1x1m25 162 16 and and CC cord-013178-li1x1m25 162 17 Tukey Tukey NNP cord-013178-li1x1m25 162 18 's 's POS cord-013178-li1x1m25 162 19 Studentized Studentized NNP cord-013178-li1x1m25 162 20 Range Range NNP cord-013178-li1x1m25 162 21 ( ( -LRB- cord-013178-li1x1m25 162 22 HSD HSD NNP cord-013178-li1x1m25 162 23 , , , cord-013178-li1x1m25 162 24 honestly honestly RB cord-013178-li1x1m25 162 25 significant significant JJ cord-013178-li1x1m25 162 26 difference difference NN cord-013178-li1x1m25 162 27 ) ) -RRB- cord-013178-li1x1m25 163 1 multiple multiple JJ cord-013178-li1x1m25 163 2 comparisons comparison NNS cord-013178-li1x1m25 163 3 test test VBP cord-013178-li1x1m25 163 4 using use VBG cord-013178-li1x1m25 163 5 the the DT cord-013178-li1x1m25 163 6 SAS SAS NNP cord-013178-li1x1m25 163 7 Enterprise Enterprise NNP cord-013178-li1x1m25 163 8 Guide Guide NNP cord-013178-li1x1m25 163 9 7.1 7.1 CD cord-013178-li1x1m25 163 10 ® ® NN cord-013178-li1x1m25 163 11 software software NN cord-013178-li1x1m25 164 1 ( ( -LRB- cord-013178-li1x1m25 164 2 SAS SAS NNP cord-013178-li1x1m25 164 3 Institute Institute NNP cord-013178-li1x1m25 164 4 Inc. Inc. NNP cord-013178-li1x1m25 164 5 , , , cord-013178-li1x1m25 164 6 Cary Cary NNP cord-013178-li1x1m25 164 7 , , , cord-013178-li1x1m25 164 8 NC NC NNP cord-013178-li1x1m25 164 9 , , , cord-013178-li1x1m25 164 10 USA USA NNP cord-013178-li1x1m25 164 11 ) ) -RRB- cord-013178-li1x1m25 164 12 . . . cord-013178-li1x1m25 165 1 A a DT cord-013178-li1x1m25 165 2 p p NN cord-013178-li1x1m25 165 3 - - HYPH cord-013178-li1x1m25 165 4 value value NN cord-013178-li1x1m25 165 5 < < XX cord-013178-li1x1m25 165 6 0.05 0.05 CD cord-013178-li1x1m25 165 7 was be VBD cord-013178-li1x1m25 165 8 considered consider VBN cord-013178-li1x1m25 165 9 significant significant JJ cord-013178-li1x1m25 165 10 . . . cord-013178-li1x1m25 166 1 The the DT cord-013178-li1x1m25 166 2 ORF3 ORF3 NNP cord-013178-li1x1m25 166 3 peptide peptide NN cord-013178-li1x1m25 166 4 N1 n1 NN cord-013178-li1x1m25 166 5 or or CC cord-013178-li1x1m25 166 6 VLP VLP NNP cord-013178-li1x1m25 166 7 of of IN cord-013178-li1x1m25 166 8 PCV2 PCV2 NNP cord-013178-li1x1m25 166 9 was be VBD cord-013178-li1x1m25 166 10 capable capable JJ cord-013178-li1x1m25 166 11 of of IN cord-013178-li1x1m25 166 12 inducing induce VBG cord-013178-li1x1m25 166 13 each each DT cord-013178-li1x1m25 166 14 specific specific JJ cord-013178-li1x1m25 166 15 antibody antibody NN cord-013178-li1x1m25 166 16 . . . cord-013178-li1x1m25 167 1 The the DT cord-013178-li1x1m25 167 2 antibody antibody NN cord-013178-li1x1m25 167 3 titer titer NN cord-013178-li1x1m25 167 4 of of IN cord-013178-li1x1m25 167 5 each each DT cord-013178-li1x1m25 167 6 post post JJ cord-013178-li1x1m25 167 7 - - JJ cord-013178-li1x1m25 167 8 immune immune JJ cord-013178-li1x1m25 167 9 serum serum NN cord-013178-li1x1m25 167 10 ( ( -LRB- cord-013178-li1x1m25 167 11 two two CD cord-013178-li1x1m25 167 12 weeks week NNS cord-013178-li1x1m25 167 13 after after IN cord-013178-li1x1m25 167 14 the the DT cord-013178-li1x1m25 167 15 fourth fourth JJ cord-013178-li1x1m25 167 16 booster booster NN cord-013178-li1x1m25 167 17 ) ) -RRB- cord-013178-li1x1m25 167 18 was be VBD cord-013178-li1x1m25 167 19 detected detect VBN cord-013178-li1x1m25 167 20 at at IN cord-013178-li1x1m25 167 21 the the DT cord-013178-li1x1m25 167 22 dilution dilution NN cord-013178-li1x1m25 167 23 1:10000 1:10000 . cord-013178-li1x1m25 167 24 ( ( -LRB- cord-013178-li1x1m25 167 25 Figure Figure NNP cord-013178-li1x1m25 167 26 S1 S1 NNP cord-013178-li1x1m25 167 27 ) ) -RRB- cord-013178-li1x1m25 167 28 . . . cord-013178-li1x1m25 168 1 The the DT cord-013178-li1x1m25 168 2 binding binding NN cord-013178-li1x1m25 168 3 of of IN cord-013178-li1x1m25 168 4 the the DT cord-013178-li1x1m25 168 5 rabbit rabbit NN cord-013178-li1x1m25 168 6 antiserum antiserum NN cord-013178-li1x1m25 168 7 to to IN cord-013178-li1x1m25 168 8 the the DT cord-013178-li1x1m25 168 9 virus virus NN cord-013178-li1x1m25 168 10 was be VBD cord-013178-li1x1m25 168 11 confirmed confirm VBN cord-013178-li1x1m25 168 12 by by IN cord-013178-li1x1m25 168 13 using use VBG cord-013178-li1x1m25 168 14 an an DT cord-013178-li1x1m25 168 15 immunofluorescence immunofluorescence NN cord-013178-li1x1m25 168 16 assay assay NN cord-013178-li1x1m25 168 17 ( ( -LRB- cord-013178-li1x1m25 168 18 IFA IFA NNP cord-013178-li1x1m25 168 19 ) ) -RRB- cord-013178-li1x1m25 168 20 ( ( -LRB- cord-013178-li1x1m25 168 21 Figure figure NN cord-013178-li1x1m25 168 22 S2 s2 NN cord-013178-li1x1m25 168 23 ) ) -RRB- cord-013178-li1x1m25 168 24 . . . cord-013178-li1x1m25 169 1 Although although IN cord-013178-li1x1m25 169 2 pre pre JJ cord-013178-li1x1m25 169 3 - - JJ cord-013178-li1x1m25 169 4 immune immune JJ cord-013178-li1x1m25 169 5 serum serum NN cord-013178-li1x1m25 169 6 had have VBD cord-013178-li1x1m25 169 7 a a DT cord-013178-li1x1m25 169 8 low low JJ cord-013178-li1x1m25 169 9 background background NN cord-013178-li1x1m25 169 10 ( ( -LRB- cord-013178-li1x1m25 169 11 OD OD NNP cord-013178-li1x1m25 169 12 405 405 CD cord-013178-li1x1m25 169 13 value value NN cord-013178-li1x1m25 169 14 less less JJR cord-013178-li1x1m25 169 15 than than IN cord-013178-li1x1m25 169 16 0.4 0.4 CD cord-013178-li1x1m25 169 17 ) ) -RRB- cord-013178-li1x1m25 169 18 at at IN cord-013178-li1x1m25 169 19 a a DT cord-013178-li1x1m25 169 20 dilution dilution NN cord-013178-li1x1m25 169 21 of of IN cord-013178-li1x1m25 169 22 1:100 1:100 CD cord-013178-li1x1m25 169 23 , , , cord-013178-li1x1m25 169 24 the the DT cord-013178-li1x1m25 169 25 pre pre JJ cord-013178-li1x1m25 169 26 - - JJ cord-013178-li1x1m25 169 27 immune immune JJ cord-013178-li1x1m25 169 28 serum serum NN cord-013178-li1x1m25 169 29 did do VBD cord-013178-li1x1m25 169 30 not not RB cord-013178-li1x1m25 169 31 bind bind VB cord-013178-li1x1m25 169 32 the the DT cord-013178-li1x1m25 169 33 virus virus NN cord-013178-li1x1m25 169 34 as as IN cord-013178-li1x1m25 169 35 detected detect VBN cord-013178-li1x1m25 169 36 by by IN cord-013178-li1x1m25 169 37 IFA IFA NNP cord-013178-li1x1m25 169 38 ( ( -LRB- cord-013178-li1x1m25 169 39 data datum NNS cord-013178-li1x1m25 169 40 not not RB cord-013178-li1x1m25 169 41 shown show VBN cord-013178-li1x1m25 169 42 ) ) -RRB- cord-013178-li1x1m25 169 43 . . . cord-013178-li1x1m25 170 1 The the DT cord-013178-li1x1m25 170 2 author author NN cord-013178-li1x1m25 170 3 checked check VBD cord-013178-li1x1m25 170 4 for for IN cord-013178-li1x1m25 170 5 the the DT cord-013178-li1x1m25 170 6 cross cross NN cord-013178-li1x1m25 170 7 - - NN cord-013178-li1x1m25 170 8 reactivity reactivity NN cord-013178-li1x1m25 170 9 of of IN cord-013178-li1x1m25 170 10 post post JJ cord-013178-li1x1m25 170 11 - - JJ cord-013178-li1x1m25 170 12 immune immune JJ cord-013178-li1x1m25 170 13 antiserum antiserum NN cord-013178-li1x1m25 170 14 to to IN cord-013178-li1x1m25 170 15 other other JJ cord-013178-li1x1m25 170 16 antigens antigen NNS cord-013178-li1x1m25 170 17 . . . cord-013178-li1x1m25 171 1 The the DT cord-013178-li1x1m25 171 2 result result NN cord-013178-li1x1m25 171 3 shows show VBZ cord-013178-li1x1m25 171 4 they -PRON- PRP cord-013178-li1x1m25 171 5 have have VBP cord-013178-li1x1m25 171 6 a a DT cord-013178-li1x1m25 171 7 minor minor JJ cord-013178-li1x1m25 171 8 cross cross JJ cord-013178-li1x1m25 171 9 - - NN cord-013178-li1x1m25 171 10 reaction reaction NN cord-013178-li1x1m25 171 11 at at IN cord-013178-li1x1m25 171 12 a a DT cord-013178-li1x1m25 171 13 dilution dilution NN cord-013178-li1x1m25 171 14 of of IN cord-013178-li1x1m25 171 15 1:100 1:100 CD cord-013178-li1x1m25 171 16 ( ( -LRB- cord-013178-li1x1m25 171 17 Figure Figure NNP cord-013178-li1x1m25 171 18 S1 S1 NNP cord-013178-li1x1m25 171 19 ) ) -RRB- cord-013178-li1x1m25 171 20 , , , cord-013178-li1x1m25 171 21 but but CC cord-013178-li1x1m25 171 22 the the DT cord-013178-li1x1m25 171 23 OD OD NNP cord-013178-li1x1m25 171 24 405 405 CD cord-013178-li1x1m25 171 25 value value NN cord-013178-li1x1m25 171 26 of of IN cord-013178-li1x1m25 171 27 cross cross JJ cord-013178-li1x1m25 171 28 - - NN cord-013178-li1x1m25 171 29 reaction reaction NN cord-013178-li1x1m25 171 30 has have VBZ cord-013178-li1x1m25 171 31 not not RB cord-013178-li1x1m25 171 32 significantly significantly RB cord-013178-li1x1m25 171 33 higher high JJR cord-013178-li1x1m25 171 34 than than IN cord-013178-li1x1m25 171 35 that that DT cord-013178-li1x1m25 171 36 of of IN cord-013178-li1x1m25 171 37 the the DT cord-013178-li1x1m25 171 38 negative negative JJ cord-013178-li1x1m25 171 39 control control NN cord-013178-li1x1m25 171 40 serum serum NN cord-013178-li1x1m25 171 41 ( ( -LRB- cord-013178-li1x1m25 171 42 p p NN cord-013178-li1x1m25 171 43 ≥ ≥ CD cord-013178-li1x1m25 171 44 0.05 0.05 CD cord-013178-li1x1m25 171 45 ) ) -RRB- cord-013178-li1x1m25 172 1 ( ( -LRB- cord-013178-li1x1m25 172 2 Figure Figure NNP cord-013178-li1x1m25 172 3 S1 S1 NNP cord-013178-li1x1m25 172 4 ) ) -RRB- cord-013178-li1x1m25 172 5 . . . cord-013178-li1x1m25 173 1 Following follow VBG cord-013178-li1x1m25 173 2 the the DT cord-013178-li1x1m25 173 3 fusions fusion NNS cord-013178-li1x1m25 173 4 , , , cord-013178-li1x1m25 173 5 hybridoma hybridoma NN cord-013178-li1x1m25 173 6 supernatants supernatant NNS cord-013178-li1x1m25 173 7 were be VBD cord-013178-li1x1m25 173 8 screened screen VBN cord-013178-li1x1m25 173 9 for for IN cord-013178-li1x1m25 173 10 the the DT cord-013178-li1x1m25 173 11 presence presence NN cord-013178-li1x1m25 173 12 of of IN cord-013178-li1x1m25 173 13 anti anti NNP cord-013178-li1x1m25 173 14 - - NNP cord-013178-li1x1m25 173 15 ORF3 ORF3 NNP cord-013178-li1x1m25 173 16 ( ( -LRB- cord-013178-li1x1m25 173 17 peptide peptide JJ cord-013178-li1x1m25 173 18 N1 n1 NN cord-013178-li1x1m25 173 19 ) ) -RRB- cord-013178-li1x1m25 173 20 specific specific JJ cord-013178-li1x1m25 173 21 antibodies antibody NNS cord-013178-li1x1m25 173 22 by by IN cord-013178-li1x1m25 173 23 iELISA iELISA NNP cord-013178-li1x1m25 173 24 . . . cord-013178-li1x1m25 174 1 Among among IN cord-013178-li1x1m25 174 2 them -PRON- PRP cord-013178-li1x1m25 174 3 , , , cord-013178-li1x1m25 174 4 34 34 CD cord-013178-li1x1m25 174 5 hybridomas hybridomas NN cord-013178-li1x1m25 174 6 supernatants supernatant NNS cord-013178-li1x1m25 174 7 reacted react VBD cord-013178-li1x1m25 174 8 with with IN cord-013178-li1x1m25 174 9 the the DT cord-013178-li1x1m25 174 10 peptide peptide NN cord-013178-li1x1m25 174 11 N1 n1 NN cord-013178-li1x1m25 174 12 at at IN cord-013178-li1x1m25 174 13 the the DT cord-013178-li1x1m25 174 14 first first JJ cord-013178-li1x1m25 174 15 screening screening NN cord-013178-li1x1m25 174 16 ( ( -LRB- cord-013178-li1x1m25 174 17 data datum NNS cord-013178-li1x1m25 174 18 not not RB cord-013178-li1x1m25 174 19 shown show VBN cord-013178-li1x1m25 174 20 ) ) -RRB- cord-013178-li1x1m25 174 21 . . . cord-013178-li1x1m25 175 1 After after IN cord-013178-li1x1m25 175 2 repeatedly repeatedly RB cord-013178-li1x1m25 175 3 subcloning subclone VBG cord-013178-li1x1m25 175 4 by by IN cord-013178-li1x1m25 175 5 the the DT cord-013178-li1x1m25 175 6 limiting limit VBG cord-013178-li1x1m25 175 7 dilution dilution NN cord-013178-li1x1m25 175 8 and and CC cord-013178-li1x1m25 175 9 selection selection NN cord-013178-li1x1m25 175 10 , , , cord-013178-li1x1m25 175 11 two two CD cord-013178-li1x1m25 175 12 stable stable JJ cord-013178-li1x1m25 175 13 hybridomas hybridomas NN cord-013178-li1x1m25 175 14 secreting secrete VBG cord-013178-li1x1m25 175 15 anti anti NNP cord-013178-li1x1m25 175 16 - - NNP cord-013178-li1x1m25 175 17 ORF3 ORF3 NNP cord-013178-li1x1m25 175 18 mAbs mAbs NNP cord-013178-li1x1m25 175 19 ( ( -LRB- cord-013178-li1x1m25 175 20 7D3 7d3 CD cord-013178-li1x1m25 175 21 and and CC cord-013178-li1x1m25 175 22 6D10 6d10 CD cord-013178-li1x1m25 175 23 ) ) -RRB- cord-013178-li1x1m25 175 24 were be VBD cord-013178-li1x1m25 175 25 obtained obtain VBN cord-013178-li1x1m25 175 26 . . . cord-013178-li1x1m25 176 1 The the DT cord-013178-li1x1m25 176 2 hybridoma hybridoma NN cord-013178-li1x1m25 176 3 produced produce VBD cord-013178-li1x1m25 176 4 IgG1 igg1 NN cord-013178-li1x1m25 176 5 mAb mAb NNP cord-013178-li1x1m25 176 6 ( ( -LRB- cord-013178-li1x1m25 176 7 7D3 7d3 CD cord-013178-li1x1m25 176 8 ) ) -RRB- cord-013178-li1x1m25 176 9 with with IN cord-013178-li1x1m25 176 10 kappa kappa NN cord-013178-li1x1m25 176 11 - - HYPH cord-013178-li1x1m25 176 12 light light JJ cord-013178-li1x1m25 176 13 chains chain NNS cord-013178-li1x1m25 176 14 . . . cord-013178-li1x1m25 177 1 The the DT cord-013178-li1x1m25 177 2 other other JJ cord-013178-li1x1m25 177 3 produced produce VBN cord-013178-li1x1m25 177 4 mAb mAb NNS cord-013178-li1x1m25 177 5 ( ( -LRB- cord-013178-li1x1m25 177 6 6D10 6d10 CD cord-013178-li1x1m25 177 7 ) ) -RRB- cord-013178-li1x1m25 177 8 with with IN cord-013178-li1x1m25 177 9 a a DT cord-013178-li1x1m25 177 10 lambda lambda NN cord-013178-li1x1m25 177 11 - - HYPH cord-013178-li1x1m25 177 12 light light JJ cord-013178-li1x1m25 177 13 chain chain NN cord-013178-li1x1m25 177 14 ( ( -LRB- cord-013178-li1x1m25 177 15 Figure figure NN cord-013178-li1x1m25 177 16 1A 1a NN cord-013178-li1x1m25 177 17 ) ) -RRB- cord-013178-li1x1m25 177 18 ; ; : cord-013178-li1x1m25 177 19 however however RB cord-013178-li1x1m25 177 20 , , , cord-013178-li1x1m25 177 21 no no DT cord-013178-li1x1m25 177 22 heavy heavy JJ cord-013178-li1x1m25 177 23 chain chain NN cord-013178-li1x1m25 177 24 was be VBD cord-013178-li1x1m25 177 25 detected detect VBN cord-013178-li1x1m25 177 26 in in IN cord-013178-li1x1m25 177 27 the the DT cord-013178-li1x1m25 177 28 supernatant supernatant NN cord-013178-li1x1m25 177 29 of of IN cord-013178-li1x1m25 177 30 clone clone NN cord-013178-li1x1m25 177 31 6D10 6d10 CD cord-013178-li1x1m25 177 32 by by IN cord-013178-li1x1m25 177 33 SBA SBA NNP cord-013178-li1x1m25 177 34 Clonotyping Clonotyping NNP cord-013178-li1x1m25 177 35 System System NNP cord-013178-li1x1m25 177 36 / / SYM cord-013178-li1x1m25 177 37 HRP HRP NNP cord-013178-li1x1m25 177 38 assay assay NN cord-013178-li1x1m25 177 39 . . . cord-013178-li1x1m25 178 1 The the DT cord-013178-li1x1m25 178 2 supernatant supernatant NN cord-013178-li1x1m25 178 3 of of IN cord-013178-li1x1m25 178 4 a a DT cord-013178-li1x1m25 178 5 hybridoma hybridoma NN cord-013178-li1x1m25 178 6 ( ( -LRB- cord-013178-li1x1m25 178 7 clone clone NN cord-013178-li1x1m25 178 8 6D10 6d10 CD cord-013178-li1x1m25 178 9 ) ) -RRB- cord-013178-li1x1m25 178 10 was be VBD cord-013178-li1x1m25 178 11 separated separate VBN cord-013178-li1x1m25 178 12 directly directly RB cord-013178-li1x1m25 178 13 by by IN cord-013178-li1x1m25 178 14 BoltTM BoltTM NNP cord-013178-li1x1m25 178 15 Bis Bis NNP cord-013178-li1x1m25 178 16 - - HYPH cord-013178-li1x1m25 178 17 Tris Tris NNP cord-013178-li1x1m25 178 18 Plus plus CC cord-013178-li1x1m25 178 19 Gel Gel NNP cord-013178-li1x1m25 178 20 to to TO cord-013178-li1x1m25 178 21 confirm confirm VB cord-013178-li1x1m25 178 22 the the DT cord-013178-li1x1m25 178 23 heavy heavy JJ cord-013178-li1x1m25 178 24 chain chain NN cord-013178-li1x1m25 178 25 of of IN cord-013178-li1x1m25 178 26 this this DT cord-013178-li1x1m25 178 27 mAb mab NN cord-013178-li1x1m25 178 28 . . . cord-013178-li1x1m25 179 1 The the DT cord-013178-li1x1m25 179 2 gel gel NN cord-013178-li1x1m25 179 3 showed show VBD cord-013178-li1x1m25 179 4 a a DT cord-013178-li1x1m25 179 5 broad broad JJ cord-013178-li1x1m25 179 6 staining staining NN cord-013178-li1x1m25 179 7 region region NN cord-013178-li1x1m25 179 8 within within IN cord-013178-li1x1m25 179 9 40 40 CD cord-013178-li1x1m25 179 10 - - SYM cord-013178-li1x1m25 179 11 98 98 CD cord-013178-li1x1m25 179 12 kDa kDa NNS cord-013178-li1x1m25 179 13 . . . cord-013178-li1x1m25 180 1 The the DT cord-013178-li1x1m25 180 2 author author NN cord-013178-li1x1m25 180 3 speculated speculate VBD cord-013178-li1x1m25 180 4 that that IN cord-013178-li1x1m25 180 5 the the DT cord-013178-li1x1m25 180 6 supernatant supernatant NN cord-013178-li1x1m25 180 7 of of IN cord-013178-li1x1m25 180 8 a a DT cord-013178-li1x1m25 180 9 hybridoma hybridoma NN cord-013178-li1x1m25 180 10 contains contain VBZ cord-013178-li1x1m25 180 11 abundant abundant JJ cord-013178-li1x1m25 180 12 serum serum NN cord-013178-li1x1m25 180 13 proteins protein NNS cord-013178-li1x1m25 180 14 from from IN cord-013178-li1x1m25 180 15 the the DT cord-013178-li1x1m25 180 16 fetal fetal JJ cord-013178-li1x1m25 180 17 bovine bovine JJ cord-013178-li1x1m25 180 18 serum serum NN cord-013178-li1x1m25 180 19 - - HYPH cord-013178-li1x1m25 180 20 containing contain VBG cord-013178-li1x1m25 180 21 medium medium NN cord-013178-li1x1m25 180 22 . . . cord-013178-li1x1m25 181 1 That that DT cord-013178-li1x1m25 181 2 is be VBZ cord-013178-li1x1m25 181 3 to to TO cord-013178-li1x1m25 181 4 say say VB cord-013178-li1x1m25 181 5 , , , cord-013178-li1x1m25 181 6 the the DT cord-013178-li1x1m25 181 7 supernatant supernatant NN cord-013178-li1x1m25 181 8 of of IN cord-013178-li1x1m25 181 9 a a DT cord-013178-li1x1m25 181 10 hybridoma hybridoma NN cord-013178-li1x1m25 181 11 ( ( -LRB- cord-013178-li1x1m25 181 12 clone clone NN cord-013178-li1x1m25 181 13 6D10 6d10 CD cord-013178-li1x1m25 181 14 ) ) -RRB- cord-013178-li1x1m25 181 15 contains contain VBZ cord-013178-li1x1m25 181 16 a a DT cord-013178-li1x1m25 181 17 little little JJ cord-013178-li1x1m25 181 18 mAb mAb NNS cord-013178-li1x1m25 181 19 6D10 6d10 CD cord-013178-li1x1m25 181 20 ( ( -LRB- cord-013178-li1x1m25 181 21 light light JJ cord-013178-li1x1m25 181 22 chain chain NN cord-013178-li1x1m25 181 23 ) ) -RRB- cord-013178-li1x1m25 181 24 . . . cord-013178-li1x1m25 182 1 Further further RB cord-013178-li1x1m25 182 2 , , , cord-013178-li1x1m25 182 3 the the DT cord-013178-li1x1m25 182 4 Western western JJ cord-013178-li1x1m25 182 5 blotting blotting NN cord-013178-li1x1m25 182 6 was be VBD cord-013178-li1x1m25 182 7 used use VBN cord-013178-li1x1m25 182 8 to to TO cord-013178-li1x1m25 182 9 confirm confirm VB cord-013178-li1x1m25 182 10 the the DT cord-013178-li1x1m25 182 11 heavy heavy JJ cord-013178-li1x1m25 182 12 chain chain NN cord-013178-li1x1m25 182 13 of of IN cord-013178-li1x1m25 182 14 mAb mab $ cord-013178-li1x1m25 182 15 6D10 6d10 CD cord-013178-li1x1m25 182 16 and and CC cord-013178-li1x1m25 182 17 its -PRON- PRP$ cord-013178-li1x1m25 182 18 molecular molecular JJ cord-013178-li1x1m25 182 19 weight weight NN cord-013178-li1x1m25 182 20 . . . cord-013178-li1x1m25 183 1 The the DT cord-013178-li1x1m25 183 2 HRP HRP NNP cord-013178-li1x1m25 183 3 - - HYPH cord-013178-li1x1m25 183 4 conjugated conjugate VBN cord-013178-li1x1m25 183 5 antibodies antibody NNS cord-013178-li1x1m25 183 6 ( ( -LRB- cord-013178-li1x1m25 183 7 goat goat NNP cord-013178-li1x1m25 183 8 anti anti JJ cord-013178-li1x1m25 183 9 - - JJ cord-013178-li1x1m25 183 10 mouse mouse NNP cord-013178-li1x1m25 183 11 IgA IgA NNP cord-013178-li1x1m25 183 12 / / SYM cord-013178-li1x1m25 183 13 IgG IgG NNP cord-013178-li1x1m25 183 14 / / SYM cord-013178-li1x1m25 183 15 IgM IgM NNP cord-013178-li1x1m25 183 16 , , , cord-013178-li1x1m25 183 17 H H NNP cord-013178-li1x1m25 183 18 / / SYM cord-013178-li1x1m25 183 19 L L NNP cord-013178-li1x1m25 183 20 chain chain NN cord-013178-li1x1m25 183 21 , , , cord-013178-li1x1m25 183 22 and and CC cord-013178-li1x1m25 183 23 goat goat NN cord-013178-li1x1m25 183 24 anti anti JJ cord-013178-li1x1m25 183 25 - - JJ cord-013178-li1x1m25 183 26 mouse mouse JJ cord-013178-li1x1m25 183 27 lambda lambda NN cord-013178-li1x1m25 183 28 light light NN cord-013178-li1x1m25 183 29 chain chain NN cord-013178-li1x1m25 183 30 ) ) -RRB- cord-013178-li1x1m25 183 31 gave give VBD cord-013178-li1x1m25 183 32 specific specific JJ cord-013178-li1x1m25 183 33 reactions reaction NNS cord-013178-li1x1m25 183 34 with with IN cord-013178-li1x1m25 183 35 the the DT cord-013178-li1x1m25 183 36 supernatant supernatant NN cord-013178-li1x1m25 183 37 . . . cord-013178-li1x1m25 184 1 Two two CD cord-013178-li1x1m25 184 2 bands band NNS cord-013178-li1x1m25 184 3 were be VBD cord-013178-li1x1m25 184 4 observed observe VBN cord-013178-li1x1m25 184 5 at at IN cord-013178-li1x1m25 184 6 about about RB cord-013178-li1x1m25 184 7 33 33 CD cord-013178-li1x1m25 184 8 ( ( -LRB- cord-013178-li1x1m25 184 9 between between IN cord-013178-li1x1m25 184 10 28 28 CD cord-013178-li1x1m25 184 11 and and CC cord-013178-li1x1m25 184 12 38 38 CD cord-013178-li1x1m25 184 13 ) ) -RRB- cord-013178-li1x1m25 184 14 and and CC cord-013178-li1x1m25 184 15 6 6 CD cord-013178-li1x1m25 184 16 kDa kDa NNS cord-013178-li1x1m25 184 17 , , , cord-013178-li1x1m25 184 18 respectively respectively RB cord-013178-li1x1m25 184 19 ( ( -LRB- cord-013178-li1x1m25 184 20 Figure figure NN cord-013178-li1x1m25 184 21 1 1 CD cord-013178-li1x1m25 184 22 ) ) -RRB- cord-013178-li1x1m25 184 23 . . . cord-013178-li1x1m25 185 1 The the DT cord-013178-li1x1m25 185 2 lower low JJR cord-013178-li1x1m25 185 3 band band NN cord-013178-li1x1m25 185 4 ( ( -LRB- cord-013178-li1x1m25 185 5 6 6 CD cord-013178-li1x1m25 185 6 kDa kDa NNPS cord-013178-li1x1m25 185 7 ) ) -RRB- cord-013178-li1x1m25 185 8 implied imply VBD cord-013178-li1x1m25 185 9 that that IN cord-013178-li1x1m25 185 10 some some DT cord-013178-li1x1m25 185 11 degradation degradation NN cord-013178-li1x1m25 185 12 of of IN cord-013178-li1x1m25 185 13 this this DT cord-013178-li1x1m25 185 14 mAb mab NN cord-013178-li1x1m25 185 15 . . . cord-013178-li1x1m25 186 1 However however RB cord-013178-li1x1m25 186 2 , , , cord-013178-li1x1m25 186 3 other other JJ cord-013178-li1x1m25 186 4 anti anti JJ cord-013178-li1x1m25 186 5 - - JJ cord-013178-li1x1m25 186 6 Ig ig JJ cord-013178-li1x1m25 186 7 antibodies antibody NNS cord-013178-li1x1m25 186 8 ( ( -LRB- cord-013178-li1x1m25 186 9 goat goat NNP cord-013178-li1x1m25 186 10 anti anti JJ cord-013178-li1x1m25 186 11 - - JJ cord-013178-li1x1m25 186 12 mouse mouse NN cord-013178-li1x1m25 186 13 Ig Ig NNP cord-013178-li1x1m25 186 14 A A NNP cord-013178-li1x1m25 186 15 ( ( -LRB- cord-013178-li1x1m25 186 16 α α NN cord-013178-li1x1m25 186 17 chain chain NN cord-013178-li1x1m25 186 18 ) ) -RRB- cord-013178-li1x1m25 186 19 , , , cord-013178-li1x1m25 186 20 goat goat NNP cord-013178-li1x1m25 186 21 anti anti JJ cord-013178-li1x1m25 186 22 - - JJ cord-013178-li1x1m25 186 23 mouse mouse JJ cord-013178-li1x1m25 186 24 IgM IgM NNP cord-013178-li1x1m25 186 25 ( ( -LRB- cord-013178-li1x1m25 186 26 µ µ NNP cord-013178-li1x1m25 186 27 chain chain NN cord-013178-li1x1m25 186 28 ) ) -RRB- cord-013178-li1x1m25 186 29 , , , cord-013178-li1x1m25 186 30 and and CC cord-013178-li1x1m25 186 31 goat goat NN cord-013178-li1x1m25 186 32 anti anti JJ cord-013178-li1x1m25 186 33 - - JJ cord-013178-li1x1m25 186 34 mouse mouse JJ cord-013178-li1x1m25 186 35 IgG igg NN cord-013178-li1x1m25 186 36 ( ( -LRB- cord-013178-li1x1m25 186 37 Fcγ fcγ NN cord-013178-li1x1m25 186 38 ) ) -RRB- cord-013178-li1x1m25 186 39 ) ) -RRB- cord-013178-li1x1m25 186 40 gave give VBD cord-013178-li1x1m25 186 41 negative negative JJ cord-013178-li1x1m25 186 42 reactions reaction NNS cord-013178-li1x1m25 186 43 with with IN cord-013178-li1x1m25 186 44 the the DT cord-013178-li1x1m25 186 45 supernatant supernatant NN cord-013178-li1x1m25 186 46 . . . cord-013178-li1x1m25 187 1 This this DT cord-013178-li1x1m25 187 2 study study NN cord-013178-li1x1m25 187 3 confirmed confirm VBD cord-013178-li1x1m25 187 4 mAb mAb NNS cord-013178-li1x1m25 187 5 6D10 6d10 CD cord-013178-li1x1m25 187 6 contains contain VBZ cord-013178-li1x1m25 187 7 a a DT cord-013178-li1x1m25 187 8 lambda lambda NN cord-013178-li1x1m25 187 9 light light NN cord-013178-li1x1m25 187 10 chain chain NN cord-013178-li1x1m25 187 11 , , , cord-013178-li1x1m25 187 12 but but CC cord-013178-li1x1m25 187 13 it -PRON- PRP cord-013178-li1x1m25 187 14 does do VBZ cord-013178-li1x1m25 187 15 not not RB cord-013178-li1x1m25 187 16 include include VB cord-013178-li1x1m25 187 17 any any DT cord-013178-li1x1m25 187 18 intact intact JJ cord-013178-li1x1m25 187 19 heavy heavy JJ cord-013178-li1x1m25 187 20 chain chain NN cord-013178-li1x1m25 187 21 . . . cord-013178-li1x1m25 188 1 Since since IN cord-013178-li1x1m25 188 2 the the DT cord-013178-li1x1m25 188 3 molecular molecular JJ cord-013178-li1x1m25 188 4 weight weight NN cord-013178-li1x1m25 188 5 of of IN cord-013178-li1x1m25 188 6 the the DT cord-013178-li1x1m25 188 7 light light JJ cord-013178-li1x1m25 188 8 chain chain NN cord-013178-li1x1m25 188 9 was be VBD cord-013178-li1x1m25 188 10 25 25 CD cord-013178-li1x1m25 188 11 kDa kDa NNS cord-013178-li1x1m25 188 12 , , , cord-013178-li1x1m25 188 13 it -PRON- PRP cord-013178-li1x1m25 188 14 may may MD cord-013178-li1x1m25 188 15 be be VB cord-013178-li1x1m25 188 16 assumed assume VBN cord-013178-li1x1m25 188 17 that that IN cord-013178-li1x1m25 188 18 the the DT cord-013178-li1x1m25 188 19 mAb mAb NNS cord-013178-li1x1m25 188 20 6D10 6d10 CD cord-013178-li1x1m25 188 21 might may MD cord-013178-li1x1m25 188 22 contain contain VB cord-013178-li1x1m25 188 23 a a DT cord-013178-li1x1m25 188 24 short short JJ cord-013178-li1x1m25 188 25 fragment fragment NN cord-013178-li1x1m25 188 26 of of IN cord-013178-li1x1m25 188 27 a a DT cord-013178-li1x1m25 188 28 protein protein NN cord-013178-li1x1m25 188 29 . . . cord-013178-li1x1m25 188 30 _SP cord-013178-li1x1m25 189 1 A a DT cord-013178-li1x1m25 189 2 peptide peptide JJ cord-013178-li1x1m25 189 3 scan scan NN cord-013178-li1x1m25 189 4 analysis analysis NN cord-013178-li1x1m25 189 5 was be VBD cord-013178-li1x1m25 189 6 performed perform VBN cord-013178-li1x1m25 189 7 to to TO cord-013178-li1x1m25 189 8 determine determine VB cord-013178-li1x1m25 189 9 the the DT cord-013178-li1x1m25 189 10 binding binding NN cord-013178-li1x1m25 189 11 site site NN cord-013178-li1x1m25 189 12 for for IN cord-013178-li1x1m25 189 13 each each DT cord-013178-li1x1m25 189 14 mAb mAb NNS cord-013178-li1x1m25 189 15 ( ( -LRB- cord-013178-li1x1m25 189 16 Figure figure NN cord-013178-li1x1m25 189 17 2 2 CD cord-013178-li1x1m25 189 18 ) ) -RRB- cord-013178-li1x1m25 189 19 . . . cord-013178-li1x1m25 190 1 The the DT cord-013178-li1x1m25 190 2 mAb mAb NNP cord-013178-li1x1m25 190 3 7D3 7D3 NNP cord-013178-li1x1m25 190 4 bound bind VBN cord-013178-li1x1m25 190 5 to to IN cord-013178-li1x1m25 190 6 two two CD cord-013178-li1x1m25 190 7 linear linear JJ cord-013178-li1x1m25 190 8 peptides peptide NNS cord-013178-li1x1m25 190 9 ( ( -LRB- cord-013178-li1x1m25 190 10 peptide peptide JJ cord-013178-li1x1m25 190 11 N1 N1 NNP cord-013178-li1x1m25 190 12 and and CC cord-013178-li1x1m25 190 13 P126 P126 NNP cord-013178-li1x1m25 190 14 ) ) -RRB- cord-013178-li1x1m25 190 15 . . . cord-013178-li1x1m25 191 1 The the DT cord-013178-li1x1m25 191 2 peptide peptide NN cord-013178-li1x1m25 191 3 N1 n1 NN cord-013178-li1x1m25 191 4 was be VBD cord-013178-li1x1m25 191 5 appended append VBN cord-013178-li1x1m25 191 6 with with IN cord-013178-li1x1m25 191 7 a a DT cord-013178-li1x1m25 191 8 cysteine cysteine NN cord-013178-li1x1m25 191 9 residue residue NN cord-013178-li1x1m25 191 10 at at IN cord-013178-li1x1m25 191 11 the the DT cord-013178-li1x1m25 191 12 N n CD cord-013178-li1x1m25 191 13 - - HYPH cord-013178-li1x1m25 191 14 terminal terminal NN cord-013178-li1x1m25 191 15 of of IN cord-013178-li1x1m25 191 16 the the DT cord-013178-li1x1m25 191 17 ORF3 ORF3 NNP cord-013178-li1x1m25 191 18 peptide peptide NN cord-013178-li1x1m25 191 19 ( ( -LRB- cord-013178-li1x1m25 191 20 residues residue NNS cord-013178-li1x1m25 191 21 35 35 CD cord-013178-li1x1m25 191 22 - - SYM cord-013178-li1x1m25 191 23 65 65 CD cord-013178-li1x1m25 191 24 , , , cord-013178-li1x1m25 191 25 P126 p126 CD cord-013178-li1x1m25 191 26 ) ) -RRB- cord-013178-li1x1m25 191 27 . . . cord-013178-li1x1m25 192 1 Both both DT cord-013178-li1x1m25 192 2 of of IN cord-013178-li1x1m25 192 3 them -PRON- PRP cord-013178-li1x1m25 192 4 contained contain VBD cord-013178-li1x1m25 192 5 the the DT cord-013178-li1x1m25 192 6 sequence sequence NN cord-013178-li1x1m25 192 7 of of IN cord-013178-li1x1m25 192 8 the the DT cord-013178-li1x1m25 192 9 ORF3 ORF3 NNP cord-013178-li1x1m25 192 10 protein protein NN cord-013178-li1x1m25 192 11 of of IN cord-013178-li1x1m25 192 12 PCV2b pcv2b NN cord-013178-li1x1m25 192 13 between between IN cord-013178-li1x1m25 192 14 residues residue NNS cord-013178-li1x1m25 192 15 35 35 CD cord-013178-li1x1m25 192 16 and and CC cord-013178-li1x1m25 192 17 66 66 CD cord-013178-li1x1m25 192 18 . . . cord-013178-li1x1m25 193 1 However however RB cord-013178-li1x1m25 193 2 , , , cord-013178-li1x1m25 193 3 the the DT cord-013178-li1x1m25 193 4 non non JJ cord-013178-li1x1m25 193 5 - - JJ cord-013178-li1x1m25 193 6 IgG igg JJ cord-013178-li1x1m25 193 7 mAb mAb NNS cord-013178-li1x1m25 193 8 ( ( -LRB- cord-013178-li1x1m25 193 9 6D10 6d10 CD cord-013178-li1x1m25 193 10 ) ) -RRB- cord-013178-li1x1m25 193 11 not not RB cord-013178-li1x1m25 193 12 only only RB cord-013178-li1x1m25 193 13 reacted react VBD cord-013178-li1x1m25 193 14 with with IN cord-013178-li1x1m25 193 15 peptide peptide NN cord-013178-li1x1m25 193 16 N1 n1 NN cord-013178-li1x1m25 193 17 but but CC cord-013178-li1x1m25 193 18 also also RB cord-013178-li1x1m25 193 19 showed show VBD cord-013178-li1x1m25 193 20 minor minor JJ cord-013178-li1x1m25 193 21 reactions reaction NNS cord-013178-li1x1m25 193 22 against against IN cord-013178-li1x1m25 193 23 other other JJ cord-013178-li1x1m25 193 24 peptides peptide NNS cord-013178-li1x1m25 193 25 , , , cord-013178-li1x1m25 193 26 which which WDT cord-013178-li1x1m25 193 27 compared compare VBN cord-013178-li1x1m25 193 28 with with IN cord-013178-li1x1m25 193 29 the the DT cord-013178-li1x1m25 193 30 mAb mAb NNP cord-013178-li1x1m25 193 31 7D3 7d3 CD cord-013178-li1x1m25 193 32 in in IN cord-013178-li1x1m25 193 33 the the DT cord-013178-li1x1m25 193 34 ELISA ELISA NNP cord-013178-li1x1m25 193 35 test test NN cord-013178-li1x1m25 193 36 ( ( -LRB- cord-013178-li1x1m25 193 37 Figure figure NN cord-013178-li1x1m25 193 38 2 2 CD cord-013178-li1x1m25 193 39 ) ) -RRB- cord-013178-li1x1m25 193 40 . . . cord-013178-li1x1m25 194 1 Interestingly interestingly RB cord-013178-li1x1m25 194 2 , , , cord-013178-li1x1m25 194 3 the the DT cord-013178-li1x1m25 194 4 anti anti JJ cord-013178-li1x1m25 194 5 - - JJ cord-013178-li1x1m25 194 6 peptide peptide JJ cord-013178-li1x1m25 194 7 N1 N1 NNP cord-013178-li1x1m25 194 8 mouse mouse NN cord-013178-li1x1m25 194 9 serum serum NN cord-013178-li1x1m25 194 10 reacted react VBD cord-013178-li1x1m25 194 11 all all DT cord-013178-li1x1m25 194 12 test test NN cord-013178-li1x1m25 194 13 peptides peptide NNS cord-013178-li1x1m25 194 14 and and CC cord-013178-li1x1m25 194 15 bound bind VBN cord-013178-li1x1m25 194 16 firmly firmly RB cord-013178-li1x1m25 194 17 to to IN cord-013178-li1x1m25 194 18 six six CD cord-013178-li1x1m25 194 19 linear linear JJ cord-013178-li1x1m25 194 20 peptides peptide NNS cord-013178-li1x1m25 194 21 ( ( -LRB- cord-013178-li1x1m25 194 22 peptide peptide JJ cord-013178-li1x1m25 194 23 N1 n1 NN cord-013178-li1x1m25 194 24 , , , cord-013178-li1x1m25 194 25 P32 P32 NNP cord-013178-li1x1m25 194 26 , , , cord-013178-li1x1m25 194 27 P33 P33 NNP cord-013178-li1x1m25 194 28 , , , cord-013178-li1x1m25 194 29 P69 P69 NNP cord-013178-li1x1m25 194 30 , , , cord-013178-li1x1m25 194 31 and and CC cord-013178-li1x1m25 194 32 P126 p126 CD cord-013178-li1x1m25 194 33 ) ) -RRB- cord-013178-li1x1m25 194 34 . . . cord-013178-li1x1m25 195 1 Notably notably RB cord-013178-li1x1m25 195 2 , , , cord-013178-li1x1m25 195 3 anti anti JJ cord-013178-li1x1m25 195 4 - - JJ cord-013178-li1x1m25 195 5 N1 N1 NNP cord-013178-li1x1m25 195 6 poly poly NNP cord-013178-li1x1m25 195 7 antibodies antibody NNS cord-013178-li1x1m25 195 8 reacted react VBD cord-013178-li1x1m25 195 9 with with IN cord-013178-li1x1m25 195 10 these these DT cord-013178-li1x1m25 195 11 peptides peptide NNS cord-013178-li1x1m25 195 12 ( ( -LRB- cord-013178-li1x1m25 195 13 expect expect VBP cord-013178-li1x1m25 195 14 P33 P33 NNP cord-013178-li1x1m25 195 15 ) ) -RRB- cord-013178-li1x1m25 195 16 which which WDT cord-013178-li1x1m25 195 17 contained contain VBD cord-013178-li1x1m25 195 18 the the DT cord-013178-li1x1m25 195 19 common common JJ cord-013178-li1x1m25 195 20 core core NN cord-013178-li1x1m25 195 21 peptide peptide NN cord-013178-li1x1m25 195 22 ( ( -LRB- cord-013178-li1x1m25 195 23 P32 P32 NNP cord-013178-li1x1m25 195 24 and and CC cord-013178-li1x1m25 195 25 45 45 CD cord-013178-li1x1m25 195 26 TLLHFPAHFQ TLLHFPAHFQ NNP cord-013178-li1x1m25 195 27 54 54 CD cord-013178-li1x1m25 195 28 ) ) -RRB- cord-013178-li1x1m25 195 29 . . . cord-013178-li1x1m25 196 1 Nevertheless nevertheless RB cord-013178-li1x1m25 196 2 , , , cord-013178-li1x1m25 196 3 this this DT cord-013178-li1x1m25 196 4 study study NN cord-013178-li1x1m25 196 5 did do VBD cord-013178-li1x1m25 196 6 not not RB cord-013178-li1x1m25 196 7 get get VB cord-013178-li1x1m25 196 8 any any DT cord-013178-li1x1m25 196 9 mAb mab NN cord-013178-li1x1m25 196 10 which which WDT cord-013178-li1x1m25 196 11 bound bind VBN cord-013178-li1x1m25 196 12 firmly firmly RB cord-013178-li1x1m25 196 13 to to IN cord-013178-li1x1m25 196 14 the the DT cord-013178-li1x1m25 196 15 peptide peptide NN cord-013178-li1x1m25 196 16 P32 P32 NNP cord-013178-li1x1m25 196 17 . . . cord-013178-li1x1m25 197 1 Viruses virus NNS cord-013178-li1x1m25 197 2 2020 2020 CD cord-013178-li1x1m25 197 3 , , , cord-013178-li1x1m25 197 4 12 12 CD cord-013178-li1x1m25 197 5 , , , cord-013178-li1x1m25 197 6 x x NN cord-013178-li1x1m25 197 7 FOR for IN cord-013178-li1x1m25 197 8 PEER peer NN cord-013178-li1x1m25 197 9 REVIEW REVIEW NNP cord-013178-li1x1m25 197 10 9 9 CD cord-013178-li1x1m25 197 11 of of IN cord-013178-li1x1m25 197 12 21 21 CD cord-013178-li1x1m25 197 13 A a DT cord-013178-li1x1m25 197 14 peptide peptide JJ cord-013178-li1x1m25 197 15 scan scan NN cord-013178-li1x1m25 197 16 analysis analysis NN cord-013178-li1x1m25 197 17 was be VBD cord-013178-li1x1m25 197 18 performed perform VBN cord-013178-li1x1m25 197 19 to to TO cord-013178-li1x1m25 197 20 determine determine VB cord-013178-li1x1m25 197 21 the the DT cord-013178-li1x1m25 197 22 binding binding NN cord-013178-li1x1m25 197 23 site site NN cord-013178-li1x1m25 197 24 for for IN cord-013178-li1x1m25 197 25 each each DT cord-013178-li1x1m25 197 26 mAb mAb NNS cord-013178-li1x1m25 197 27 ( ( -LRB- cord-013178-li1x1m25 197 28 Figure figure NN cord-013178-li1x1m25 197 29 2 2 CD cord-013178-li1x1m25 197 30 ) ) -RRB- cord-013178-li1x1m25 197 31 . . . cord-013178-li1x1m25 198 1 The the DT cord-013178-li1x1m25 198 2 mAb mAb NNP cord-013178-li1x1m25 198 3 7D3 7D3 NNP cord-013178-li1x1m25 198 4 bound bind VBN cord-013178-li1x1m25 198 5 to to IN cord-013178-li1x1m25 198 6 two two CD cord-013178-li1x1m25 198 7 linear linear JJ cord-013178-li1x1m25 198 8 peptides peptide NNS cord-013178-li1x1m25 198 9 ( ( -LRB- cord-013178-li1x1m25 198 10 peptide peptide JJ cord-013178-li1x1m25 198 11 N1 N1 NNP cord-013178-li1x1m25 198 12 and and CC cord-013178-li1x1m25 198 13 P126 P126 NNP cord-013178-li1x1m25 198 14 ) ) -RRB- cord-013178-li1x1m25 198 15 . . . cord-013178-li1x1m25 199 1 The the DT cord-013178-li1x1m25 199 2 peptide peptide NN cord-013178-li1x1m25 199 3 N1 n1 NN cord-013178-li1x1m25 199 4 was be VBD cord-013178-li1x1m25 199 5 appended append VBN cord-013178-li1x1m25 199 6 with with IN cord-013178-li1x1m25 199 7 a a DT cord-013178-li1x1m25 199 8 cysteine cysteine NN cord-013178-li1x1m25 199 9 residue residue NN cord-013178-li1x1m25 199 10 at at IN cord-013178-li1x1m25 199 11 the the DT cord-013178-li1x1m25 199 12 N n CD cord-013178-li1x1m25 199 13 - - HYPH cord-013178-li1x1m25 199 14 terminal terminal NN cord-013178-li1x1m25 199 15 of of IN cord-013178-li1x1m25 199 16 the the DT cord-013178-li1x1m25 199 17 ORF3 ORF3 NNP cord-013178-li1x1m25 199 18 peptide peptide NN cord-013178-li1x1m25 199 19 ( ( -LRB- cord-013178-li1x1m25 199 20 residues residue NNS cord-013178-li1x1m25 199 21 35 35 CD cord-013178-li1x1m25 199 22 - - SYM cord-013178-li1x1m25 199 23 65 65 CD cord-013178-li1x1m25 199 24 , , , cord-013178-li1x1m25 199 25 P126 p126 CD cord-013178-li1x1m25 199 26 ) ) -RRB- cord-013178-li1x1m25 199 27 . . . cord-013178-li1x1m25 200 1 Both both DT cord-013178-li1x1m25 200 2 of of IN cord-013178-li1x1m25 200 3 them -PRON- PRP cord-013178-li1x1m25 200 4 contained contain VBD cord-013178-li1x1m25 200 5 the the DT cord-013178-li1x1m25 200 6 sequence sequence NN cord-013178-li1x1m25 200 7 of of IN cord-013178-li1x1m25 200 8 the the DT cord-013178-li1x1m25 200 9 ORF3 ORF3 NNP cord-013178-li1x1m25 200 10 protein protein NN cord-013178-li1x1m25 200 11 of of IN cord-013178-li1x1m25 200 12 PCV2b pcv2b NN cord-013178-li1x1m25 200 13 between between IN cord-013178-li1x1m25 200 14 residues residue NNS cord-013178-li1x1m25 200 15 35 35 CD cord-013178-li1x1m25 200 16 and and CC cord-013178-li1x1m25 200 17 66 66 CD cord-013178-li1x1m25 200 18 . . . cord-013178-li1x1m25 201 1 However however RB cord-013178-li1x1m25 201 2 , , , cord-013178-li1x1m25 201 3 the the DT cord-013178-li1x1m25 201 4 non non JJ cord-013178-li1x1m25 201 5 - - JJ cord-013178-li1x1m25 201 6 IgG igg JJ cord-013178-li1x1m25 201 7 mAb mAb NNS cord-013178-li1x1m25 201 8 ( ( -LRB- cord-013178-li1x1m25 201 9 6D10 6d10 CD cord-013178-li1x1m25 201 10 ) ) -RRB- cord-013178-li1x1m25 201 11 not not RB cord-013178-li1x1m25 201 12 only only RB cord-013178-li1x1m25 201 13 reacted react VBD cord-013178-li1x1m25 201 14 with with IN cord-013178-li1x1m25 201 15 peptide peptide NN cord-013178-li1x1m25 201 16 N1 n1 NN cord-013178-li1x1m25 201 17 but but CC cord-013178-li1x1m25 201 18 also also RB cord-013178-li1x1m25 201 19 showed show VBD cord-013178-li1x1m25 201 20 minor minor JJ cord-013178-li1x1m25 201 21 reactions reaction NNS cord-013178-li1x1m25 201 22 against against IN cord-013178-li1x1m25 201 23 other other JJ cord-013178-li1x1m25 201 24 peptides peptide NNS cord-013178-li1x1m25 201 25 , , , cord-013178-li1x1m25 201 26 which which WDT cord-013178-li1x1m25 201 27 compared compare VBN cord-013178-li1x1m25 201 28 with with IN cord-013178-li1x1m25 201 29 the the DT cord-013178-li1x1m25 201 30 mAb mAb NNP cord-013178-li1x1m25 201 31 7D3 7d3 CD cord-013178-li1x1m25 201 32 in in IN cord-013178-li1x1m25 201 33 the the DT cord-013178-li1x1m25 201 34 ELISA ELISA NNP cord-013178-li1x1m25 201 35 test test NN cord-013178-li1x1m25 201 36 ( ( -LRB- cord-013178-li1x1m25 201 37 Figure figure NN cord-013178-li1x1m25 201 38 2 2 CD cord-013178-li1x1m25 201 39 ) ) -RRB- cord-013178-li1x1m25 201 40 . . . cord-013178-li1x1m25 202 1 Interestingly interestingly RB cord-013178-li1x1m25 202 2 , , , cord-013178-li1x1m25 202 3 the the DT cord-013178-li1x1m25 202 4 antipeptide antipeptide NNP cord-013178-li1x1m25 202 5 N1 N1 NNP cord-013178-li1x1m25 202 6 mouse mouse NN cord-013178-li1x1m25 202 7 serum serum NN cord-013178-li1x1m25 202 8 reacted react VBD cord-013178-li1x1m25 202 9 all all DT cord-013178-li1x1m25 202 10 test test NN cord-013178-li1x1m25 202 11 peptides peptide NNS cord-013178-li1x1m25 202 12 and and CC cord-013178-li1x1m25 202 13 bound bind VBN cord-013178-li1x1m25 202 14 firmly firmly RB cord-013178-li1x1m25 202 15 to to IN cord-013178-li1x1m25 202 16 six six CD cord-013178-li1x1m25 202 17 linear linear JJ cord-013178-li1x1m25 202 18 peptides peptide NNS cord-013178-li1x1m25 202 19 ( ( -LRB- cord-013178-li1x1m25 202 20 peptide peptide JJ cord-013178-li1x1m25 202 21 N1 n1 NN cord-013178-li1x1m25 202 22 , , , cord-013178-li1x1m25 202 23 P32 P32 NNP cord-013178-li1x1m25 202 24 , , , cord-013178-li1x1m25 202 25 P33 P33 NNP cord-013178-li1x1m25 202 26 , , , cord-013178-li1x1m25 202 27 P69 P69 NNP cord-013178-li1x1m25 202 28 , , , cord-013178-li1x1m25 202 29 and and CC cord-013178-li1x1m25 202 30 P126 p126 CD cord-013178-li1x1m25 202 31 ) ) -RRB- cord-013178-li1x1m25 202 32 . . . cord-013178-li1x1m25 203 1 Notably notably RB cord-013178-li1x1m25 203 2 , , , cord-013178-li1x1m25 203 3 anti anti JJ cord-013178-li1x1m25 203 4 - - JJ cord-013178-li1x1m25 203 5 N1 N1 NNP cord-013178-li1x1m25 203 6 poly poly NNP cord-013178-li1x1m25 203 7 antibodies antibody NNS cord-013178-li1x1m25 203 8 reacted react VBD cord-013178-li1x1m25 203 9 with with IN cord-013178-li1x1m25 203 10 these these DT cord-013178-li1x1m25 203 11 peptides peptide NNS cord-013178-li1x1m25 203 12 ( ( -LRB- cord-013178-li1x1m25 203 13 expect expect VBP cord-013178-li1x1m25 203 14 P33 P33 NNP cord-013178-li1x1m25 203 15 ) ) -RRB- cord-013178-li1x1m25 203 16 which which WDT cord-013178-li1x1m25 203 17 contained contain VBD cord-013178-li1x1m25 203 18 the the DT cord-013178-li1x1m25 203 19 common common JJ cord-013178-li1x1m25 203 20 core core NN cord-013178-li1x1m25 203 21 peptide peptide NN cord-013178-li1x1m25 203 22 ( ( -LRB- cord-013178-li1x1m25 203 23 P32 P32 NNP cord-013178-li1x1m25 203 24 and and CC cord-013178-li1x1m25 203 25 45TLLHFPAHFQ54 45tllhfpahfq54 CD cord-013178-li1x1m25 203 26 ) ) -RRB- cord-013178-li1x1m25 203 27 . . . cord-013178-li1x1m25 204 1 Nevertheless nevertheless RB cord-013178-li1x1m25 204 2 , , , cord-013178-li1x1m25 204 3 this this DT cord-013178-li1x1m25 204 4 study study NN cord-013178-li1x1m25 204 5 did do VBD cord-013178-li1x1m25 204 6 not not RB cord-013178-li1x1m25 204 7 get get VB cord-013178-li1x1m25 204 8 any any DT cord-013178-li1x1m25 204 9 mAb mab NN cord-013178-li1x1m25 204 10 which which WDT cord-013178-li1x1m25 204 11 bound bind VBN cord-013178-li1x1m25 204 12 firmly firmly RB cord-013178-li1x1m25 204 13 to to IN cord-013178-li1x1m25 204 14 the the DT cord-013178-li1x1m25 204 15 peptide peptide NN cord-013178-li1x1m25 204 16 P32 P32 NNP cord-013178-li1x1m25 204 17 . . . cord-013178-li1x1m25 205 1 Anti Anti NNP cord-013178-li1x1m25 205 2 - - NNP cord-013178-li1x1m25 205 3 ORF3 ORF3 NNP cord-013178-li1x1m25 205 4 mAbs mAbs NNP cord-013178-li1x1m25 205 5 ( ( -LRB- cord-013178-li1x1m25 205 6 7D3 7d3 CD cord-013178-li1x1m25 205 7 and and CC cord-013178-li1x1m25 205 8 6D10 6d10 CD cord-013178-li1x1m25 205 9 ) ) -RRB- cord-013178-li1x1m25 205 10 bound bind VBD cord-013178-li1x1m25 205 11 the the DT cord-013178-li1x1m25 205 12 linear linear JJ cord-013178-li1x1m25 205 13 peptide peptide NN cord-013178-li1x1m25 205 14 spanning span VBG cord-013178-li1x1m25 205 15 from from IN cord-013178-li1x1m25 205 16 residues residue NNS cord-013178-li1x1m25 205 17 35 35 CD cord-013178-li1x1m25 205 18 to to IN cord-013178-li1x1m25 205 19 66 66 CD cord-013178-li1x1m25 205 20 . . . cord-013178-li1x1m25 206 1 The the DT cord-013178-li1x1m25 206 2 antipeptide antipeptide JJ cord-013178-li1x1m25 206 3 N1 N1 NNP cord-013178-li1x1m25 206 4 mouse mouse NN cord-013178-li1x1m25 206 5 serum serum NN cord-013178-li1x1m25 206 6 was be VBD cord-013178-li1x1m25 206 7 used use VBN cord-013178-li1x1m25 206 8 as as IN cord-013178-li1x1m25 206 9 the the DT cord-013178-li1x1m25 206 10 positive positive JJ cord-013178-li1x1m25 206 11 control control NN cord-013178-li1x1m25 206 12 , , , cord-013178-li1x1m25 206 13 and and CC cord-013178-li1x1m25 206 14 the the DT cord-013178-li1x1m25 206 15 anti anti JJ cord-013178-li1x1m25 206 16 - - JJ cord-013178-li1x1m25 206 17 ORF2 orf2 JJ cord-013178-li1x1m25 206 18 ( ( -LRB- cord-013178-li1x1m25 206 19 peptide peptide NN cord-013178-li1x1m25 206 20 C3 c3 NN cord-013178-li1x1m25 206 21 ) ) -RRB- cord-013178-li1x1m25 206 22 mAb mab NN cord-013178-li1x1m25 206 23 ( ( -LRB- cord-013178-li1x1m25 206 24 1H3 1h3 CD cord-013178-li1x1m25 206 25 ) ) -RRB- cord-013178-li1x1m25 206 26 served serve VBD cord-013178-li1x1m25 206 27 as as IN cord-013178-li1x1m25 206 28 the the DT cord-013178-li1x1m25 206 29 negative negative JJ cord-013178-li1x1m25 206 30 control control NN cord-013178-li1x1m25 206 31 . . . cord-013178-li1x1m25 207 1 The the DT cord-013178-li1x1m25 207 2 mAb mab NN cord-013178-li1x1m25 207 3 binding binding NN cord-013178-li1x1m25 207 4 was be VBD cord-013178-li1x1m25 207 5 tested test VBN cord-013178-li1x1m25 207 6 by by IN cord-013178-li1x1m25 207 7 using use VBG cord-013178-li1x1m25 207 8 an an DT cord-013178-li1x1m25 207 9 iELISA ielisa NN cord-013178-li1x1m25 207 10 . . . cord-013178-li1x1m25 208 1 Peptides peptide NNS cord-013178-li1x1m25 208 2 contained contain VBD cord-013178-li1x1m25 208 3 the the DT cord-013178-li1x1m25 208 4 sequence sequence NN cord-013178-li1x1m25 208 5 of of IN cord-013178-li1x1m25 208 6 the the DT cord-013178-li1x1m25 208 7 ORF3 ORF3 NNP cord-013178-li1x1m25 208 8 protein protein NN cord-013178-li1x1m25 208 9 between between IN cord-013178-li1x1m25 208 10 residues residue NNS cord-013178-li1x1m25 208 11 35 35 CD cord-013178-li1x1m25 208 12 and and CC cord-013178-li1x1m25 208 13 66 66 CD cord-013178-li1x1m25 208 14 , , , cord-013178-li1x1m25 208 15 associated associated JJ cord-013178-li1x1m25 208 16 10-mer 10-mer CD cord-013178-li1x1m25 208 17 peptides peptide NNS cord-013178-li1x1m25 208 18 , , , cord-013178-li1x1m25 208 19 truncated truncated JJ cord-013178-li1x1m25 208 20 derivatives derivative NNS cord-013178-li1x1m25 208 21 , , , cord-013178-li1x1m25 208 22 and and CC cord-013178-li1x1m25 208 23 the the DT cord-013178-li1x1m25 208 24 control control NN cord-013178-li1x1m25 208 25 ( ( -LRB- cord-013178-li1x1m25 208 26 peptide peptide NN cord-013178-li1x1m25 208 27 C3 c3 NN cord-013178-li1x1m25 208 28 ) ) -RRB- cord-013178-li1x1m25 208 29 ( ( -LRB- cord-013178-li1x1m25 208 30 as as IN cord-013178-li1x1m25 208 31 shown show VBN cord-013178-li1x1m25 208 32 in in IN cord-013178-li1x1m25 208 33 Table Table NNP cord-013178-li1x1m25 208 34 1 1 CD cord-013178-li1x1m25 208 35 ) ) -RRB- cord-013178-li1x1m25 208 36 . . . cord-013178-li1x1m25 209 1 The the DT cord-013178-li1x1m25 209 2 experiments experiment NNS cord-013178-li1x1m25 209 3 were be VBD cord-013178-li1x1m25 209 4 performed perform VBN cord-013178-li1x1m25 209 5 three three CD cord-013178-li1x1m25 209 6 times time NNS cord-013178-li1x1m25 209 7 . . . cord-013178-li1x1m25 210 1 Data datum NNS cord-013178-li1x1m25 210 2 represent represent VBP cord-013178-li1x1m25 210 3 the the DT cord-013178-li1x1m25 210 4 mean mean JJ cord-013178-li1x1m25 210 5 ± ± NNP cord-013178-li1x1m25 210 6 standard standard JJ cord-013178-li1x1m25 210 7 error error NN cord-013178-li1x1m25 210 8 ( ( -LRB- cord-013178-li1x1m25 210 9 SE SE NNP cord-013178-li1x1m25 210 10 ) ) -RRB- cord-013178-li1x1m25 210 11 . . . cord-013178-li1x1m25 211 1 For for IN cord-013178-li1x1m25 211 2 these these DT cord-013178-li1x1m25 211 3 homemade homemade JJ cord-013178-li1x1m25 211 4 PBMCs pbmc NNS cord-013178-li1x1m25 211 5 slides slide NNS cord-013178-li1x1m25 211 6 , , , cord-013178-li1x1m25 211 7 all all DT cord-013178-li1x1m25 211 8 slides slide NNS cord-013178-li1x1m25 211 9 were be VBD cord-013178-li1x1m25 211 10 made make VBN cord-013178-li1x1m25 211 11 from from IN cord-013178-li1x1m25 211 12 the the DT cord-013178-li1x1m25 211 13 same same JJ cord-013178-li1x1m25 211 14 batch batch NN cord-013178-li1x1m25 211 15 and and CC cord-013178-li1x1m25 211 16 contained contain VBD cord-013178-li1x1m25 211 17 the the DT cord-013178-li1x1m25 211 18 same same JJ cord-013178-li1x1m25 211 19 proportion proportion NN cord-013178-li1x1m25 211 20 of of IN cord-013178-li1x1m25 211 21 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 211 22 cells cell NNS cord-013178-li1x1m25 211 23 . . . cord-013178-li1x1m25 212 1 All all DT cord-013178-li1x1m25 212 2 wells well NNS cord-013178-li1x1m25 212 3 contained contain VBD cord-013178-li1x1m25 212 4 both both DT cord-013178-li1x1m25 212 5 negative negative JJ cord-013178-li1x1m25 212 6 and and CC cord-013178-li1x1m25 212 7 positive positive JJ cord-013178-li1x1m25 212 8 cells cell NNS cord-013178-li1x1m25 212 9 ( ( -LRB- cord-013178-li1x1m25 212 10 PCV2infected pcv2infected JJ cord-013178-li1x1m25 212 11 cells cell NNS cord-013178-li1x1m25 212 12 ) ) -RRB- cord-013178-li1x1m25 212 13 confirmed confirm VBN cord-013178-li1x1m25 212 14 by by IN cord-013178-li1x1m25 212 15 IFA IFA NNP cord-013178-li1x1m25 212 16 , , , cord-013178-li1x1m25 212 17 as as IN cord-013178-li1x1m25 212 18 previously previously RB cord-013178-li1x1m25 212 19 noted note VBN cord-013178-li1x1m25 212 20 in in IN cord-013178-li1x1m25 212 21 a a DT cord-013178-li1x1m25 212 22 similar similar JJ cord-013178-li1x1m25 212 23 study study NN cord-013178-li1x1m25 212 24 [ [ -LRB- cord-013178-li1x1m25 212 25 49 49 CD cord-013178-li1x1m25 212 26 ] ] -RRB- cord-013178-li1x1m25 212 27 . . . cord-013178-li1x1m25 213 1 In in IN cord-013178-li1x1m25 213 2 this this DT cord-013178-li1x1m25 213 3 study study NN cord-013178-li1x1m25 213 4 , , , cord-013178-li1x1m25 213 5 PCV2 pcv2 JJ cord-013178-li1x1m25 213 6 viral viral JJ cord-013178-li1x1m25 213 7 protein protein NN cord-013178-li1x1m25 213 8 ( ( -LRB- cord-013178-li1x1m25 213 9 ORF3 ORF3 NNP cord-013178-li1x1m25 213 10 protein protein NN cord-013178-li1x1m25 213 11 and and CC cord-013178-li1x1m25 213 12 capsid capsid NN cord-013178-li1x1m25 213 13 protein protein NN cord-013178-li1x1m25 213 14 ) ) -RRB- cord-013178-li1x1m25 213 15 locations location NNS cord-013178-li1x1m25 213 16 were be VBD cord-013178-li1x1m25 213 17 initially initially RB cord-013178-li1x1m25 213 18 assessed assess VBN cord-013178-li1x1m25 213 19 by by IN cord-013178-li1x1m25 213 20 IFA IFA NNP cord-013178-li1x1m25 213 21 . . . cord-013178-li1x1m25 214 1 The the DT cord-013178-li1x1m25 214 2 previous previous JJ cord-013178-li1x1m25 214 3 study study NN cord-013178-li1x1m25 214 4 indicated indicate VBD cord-013178-li1x1m25 214 5 that that IN cord-013178-li1x1m25 214 6 the the DT cord-013178-li1x1m25 214 7 mAb mAb NNS cord-013178-li1x1m25 214 8 1H3 1H3 NNP cord-013178-li1x1m25 214 9 bound bind VBD cord-013178-li1x1m25 214 10 the the DT cord-013178-li1x1m25 214 11 three three CD cord-013178-li1x1m25 214 12 minimal minimal JJ cord-013178-li1x1m25 214 13 linear linear JJ cord-013178-li1x1m25 214 14 epitopes epitope NNS cord-013178-li1x1m25 214 15 ( ( -LRB- cord-013178-li1x1m25 214 16 DPPLNP DPPLNP NNP cord-013178-li1x1m25 214 17 , , , cord-013178-li1x1m25 214 18 DPPLNPK DPPLNPK NNP cord-013178-li1x1m25 214 19 , , , cord-013178-li1x1m25 214 20 and and CC cord-013178-li1x1m25 214 21 LKDPPLKP LKDPPLKP NNP cord-013178-li1x1m25 214 22 ) ) -RRB- cord-013178-li1x1m25 214 23 , , , cord-013178-li1x1m25 214 24 which which WDT cord-013178-li1x1m25 214 25 were be VBD cord-013178-li1x1m25 214 26 located locate VBN cord-013178-li1x1m25 214 27 at at IN cord-013178-li1x1m25 214 28 carboxyl carboxyl NN cord-013178-li1x1m25 214 29 - - HYPH cord-013178-li1x1m25 214 30 terminus terminus NN cord-013178-li1x1m25 214 31 ( ( -LRB- cord-013178-li1x1m25 214 32 Cterminus Cterminus NNP cord-013178-li1x1m25 214 33 ) ) -RRB- cord-013178-li1x1m25 214 34 of of IN cord-013178-li1x1m25 214 35 the the DT cord-013178-li1x1m25 214 36 capsid capsid NN cord-013178-li1x1m25 214 37 proteins protein NNS cord-013178-li1x1m25 214 38 of of IN cord-013178-li1x1m25 214 39 PCV2b pcv2b NN cord-013178-li1x1m25 214 40 , , , cord-013178-li1x1m25 214 41 PCV2d PCV2d NNP cord-013178-li1x1m25 214 42 , , , cord-013178-li1x1m25 214 43 and and CC cord-013178-li1x1m25 214 44 PCV2a PCV2a NNP cord-013178-li1x1m25 214 45 , , , cord-013178-li1x1m25 214 46 respectively respectively RB cord-013178-li1x1m25 214 47 [ [ -LRB- cord-013178-li1x1m25 214 48 49 49 CD cord-013178-li1x1m25 214 49 ] ] -RRB- cord-013178-li1x1m25 214 50 . . . cord-013178-li1x1m25 215 1 Likewise likewise RB cord-013178-li1x1m25 215 2 , , , cord-013178-li1x1m25 215 3 the the DT cord-013178-li1x1m25 215 4 mAb mAb NNS cord-013178-li1x1m25 215 5 6B8 6b8 RB cord-013178-li1x1m25 215 6 bound bind VBD cord-013178-li1x1m25 215 7 two two CD cord-013178-li1x1m25 215 8 minimal minimal JJ cord-013178-li1x1m25 215 9 linear linear JJ cord-013178-li1x1m25 215 10 epitopes epitope NNS cord-013178-li1x1m25 215 11 ( ( -LRB- cord-013178-li1x1m25 215 12 KDPPLNP KDPPLNP NNP cord-013178-li1x1m25 215 13 and and CC cord-013178-li1x1m25 215 14 KDPPLNPK KDPPLNPK NNP cord-013178-li1x1m25 215 15 ) ) -RRB- cord-013178-li1x1m25 215 16 , , , cord-013178-li1x1m25 215 17 which which WDT cord-013178-li1x1m25 215 18 were be VBD cord-013178-li1x1m25 215 19 located locate VBN cord-013178-li1x1m25 215 20 at at IN cord-013178-li1x1m25 215 21 Cterminus Cterminus NNP cord-013178-li1x1m25 215 22 of of IN cord-013178-li1x1m25 215 23 the the DT cord-013178-li1x1m25 215 24 capsid capsid NN cord-013178-li1x1m25 215 25 proteins protein NNS cord-013178-li1x1m25 215 26 of of IN cord-013178-li1x1m25 215 27 PCV2b pcv2b NN cord-013178-li1x1m25 215 28 , , , cord-013178-li1x1m25 215 29 and and CC cord-013178-li1x1m25 215 30 PCV2d PCV2d NNS cord-013178-li1x1m25 215 31 , , , cord-013178-li1x1m25 215 32 respectively respectively RB cord-013178-li1x1m25 215 33 [ [ -LRB- cord-013178-li1x1m25 215 34 49 49 CD cord-013178-li1x1m25 215 35 ] ] -RRB- cord-013178-li1x1m25 215 36 . . . cord-013178-li1x1m25 216 1 First first RB cord-013178-li1x1m25 216 2 , , , cord-013178-li1x1m25 216 3 the the DT cord-013178-li1x1m25 216 4 percentage percentage NN cord-013178-li1x1m25 216 5 was be VBD cord-013178-li1x1m25 216 6 determined determine VBN cord-013178-li1x1m25 216 7 by by IN cord-013178-li1x1m25 216 8 counting count VBG cord-013178-li1x1m25 216 9 the the DT cord-013178-li1x1m25 216 10 number number NN cord-013178-li1x1m25 216 11 of of IN cord-013178-li1x1m25 216 12 positive positive JJ cord-013178-li1x1m25 216 13 cells cell NNS cord-013178-li1x1m25 216 14 per per IN cord-013178-li1x1m25 216 15 100 100 CD cord-013178-li1x1m25 216 16 nuclei nucleus NNS cord-013178-li1x1m25 216 17 in in IN cord-013178-li1x1m25 216 18 an an DT cord-013178-li1x1m25 216 19 IFA IFA NNP cord-013178-li1x1m25 216 20 slide slide NN cord-013178-li1x1m25 216 21 . . . cord-013178-li1x1m25 217 1 The the DT cord-013178-li1x1m25 217 2 data datum NNS cord-013178-li1x1m25 217 3 show show VBP cord-013178-li1x1m25 217 4 that that IN cord-013178-li1x1m25 217 5 78 78 CD cord-013178-li1x1m25 217 6 - - SYM cord-013178-li1x1m25 217 7 82 82 CD cord-013178-li1x1m25 217 8 % % NN cord-013178-li1x1m25 217 9 of of IN cord-013178-li1x1m25 217 10 PBMCs pbmc NNS cord-013178-li1x1m25 217 11 were be VBD cord-013178-li1x1m25 217 12 positive positive JJ cord-013178-li1x1m25 217 13 for for IN cord-013178-li1x1m25 217 14 anti anti JJ cord-013178-li1x1m25 217 15 - - JJ cord-013178-li1x1m25 217 16 capsid capsid JJ cord-013178-li1x1m25 217 17 peptide peptide NN cord-013178-li1x1m25 217 18 mAb mAb NNS cord-013178-li1x1m25 217 19 ( ( -LRB- cord-013178-li1x1m25 217 20 1H3 1h3 CD cord-013178-li1x1m25 217 21 ) ) -RRB- cord-013178-li1x1m25 217 22 staining staining NN cord-013178-li1x1m25 217 23 ( ( -LRB- cord-013178-li1x1m25 218 1 PCV2a- PCV2a- NNP cord-013178-li1x1m25 218 2 , , , cord-013178-li1x1m25 219 1 PCV2b- PCV2b- NNP cord-013178-li1x1m25 219 2 , , , cord-013178-li1x1m25 219 3 The the DT cord-013178-li1x1m25 219 4 anti anti JJ cord-013178-li1x1m25 219 5 - - JJ cord-013178-li1x1m25 219 6 peptide peptide JJ cord-013178-li1x1m25 219 7 N1 N1 NNP cord-013178-li1x1m25 219 8 mouse mouse NN cord-013178-li1x1m25 219 9 serum serum NN cord-013178-li1x1m25 219 10 was be VBD cord-013178-li1x1m25 219 11 used use VBN cord-013178-li1x1m25 219 12 as as IN cord-013178-li1x1m25 219 13 the the DT cord-013178-li1x1m25 219 14 positive positive JJ cord-013178-li1x1m25 219 15 control control NN cord-013178-li1x1m25 219 16 , , , cord-013178-li1x1m25 219 17 and and CC cord-013178-li1x1m25 219 18 the the DT cord-013178-li1x1m25 219 19 anti anti JJ cord-013178-li1x1m25 219 20 - - JJ cord-013178-li1x1m25 219 21 ORF2 orf2 JJ cord-013178-li1x1m25 219 22 ( ( -LRB- cord-013178-li1x1m25 219 23 peptide peptide NN cord-013178-li1x1m25 219 24 C3 c3 NN cord-013178-li1x1m25 219 25 ) ) -RRB- cord-013178-li1x1m25 219 26 mAb mab NN cord-013178-li1x1m25 219 27 ( ( -LRB- cord-013178-li1x1m25 219 28 1H3 1h3 CD cord-013178-li1x1m25 219 29 ) ) -RRB- cord-013178-li1x1m25 219 30 served serve VBD cord-013178-li1x1m25 219 31 as as IN cord-013178-li1x1m25 219 32 the the DT cord-013178-li1x1m25 219 33 negative negative JJ cord-013178-li1x1m25 219 34 control control NN cord-013178-li1x1m25 219 35 . . . cord-013178-li1x1m25 220 1 The the DT cord-013178-li1x1m25 220 2 mAb mab NN cord-013178-li1x1m25 220 3 binding binding NN cord-013178-li1x1m25 220 4 was be VBD cord-013178-li1x1m25 220 5 tested test VBN cord-013178-li1x1m25 220 6 by by IN cord-013178-li1x1m25 220 7 using use VBG cord-013178-li1x1m25 220 8 an an DT cord-013178-li1x1m25 220 9 iELISA ielisa NN cord-013178-li1x1m25 220 10 . . . cord-013178-li1x1m25 221 1 Peptides peptide NNS cord-013178-li1x1m25 221 2 contained contain VBD cord-013178-li1x1m25 221 3 the the DT cord-013178-li1x1m25 221 4 sequence sequence NN cord-013178-li1x1m25 221 5 of of IN cord-013178-li1x1m25 221 6 the the DT cord-013178-li1x1m25 221 7 ORF3 ORF3 NNP cord-013178-li1x1m25 221 8 protein protein NN cord-013178-li1x1m25 221 9 between between IN cord-013178-li1x1m25 221 10 residues residue NNS cord-013178-li1x1m25 221 11 35 35 CD cord-013178-li1x1m25 221 12 and and CC cord-013178-li1x1m25 221 13 66 66 CD cord-013178-li1x1m25 221 14 , , , cord-013178-li1x1m25 221 15 associated associated JJ cord-013178-li1x1m25 221 16 10-mer 10-mer CD cord-013178-li1x1m25 221 17 peptides peptide NNS cord-013178-li1x1m25 221 18 , , , cord-013178-li1x1m25 221 19 truncated truncated JJ cord-013178-li1x1m25 221 20 derivatives derivative NNS cord-013178-li1x1m25 221 21 , , , cord-013178-li1x1m25 221 22 and and CC cord-013178-li1x1m25 221 23 the the DT cord-013178-li1x1m25 221 24 control control NN cord-013178-li1x1m25 221 25 ( ( -LRB- cord-013178-li1x1m25 221 26 peptide peptide NN cord-013178-li1x1m25 221 27 C3 c3 NN cord-013178-li1x1m25 221 28 ) ) -RRB- cord-013178-li1x1m25 221 29 ( ( -LRB- cord-013178-li1x1m25 221 30 as as IN cord-013178-li1x1m25 221 31 shown show VBN cord-013178-li1x1m25 221 32 in in IN cord-013178-li1x1m25 221 33 Table Table NNP cord-013178-li1x1m25 221 34 1 1 CD cord-013178-li1x1m25 221 35 ) ) -RRB- cord-013178-li1x1m25 221 36 . . . cord-013178-li1x1m25 222 1 The the DT cord-013178-li1x1m25 222 2 experiments experiment NNS cord-013178-li1x1m25 222 3 were be VBD cord-013178-li1x1m25 222 4 performed perform VBN cord-013178-li1x1m25 222 5 three three CD cord-013178-li1x1m25 222 6 times time NNS cord-013178-li1x1m25 222 7 . . . cord-013178-li1x1m25 223 1 Data datum NNS cord-013178-li1x1m25 223 2 represent represent VBP cord-013178-li1x1m25 223 3 the the DT cord-013178-li1x1m25 223 4 mean mean JJ cord-013178-li1x1m25 223 5 ± ± NNP cord-013178-li1x1m25 223 6 standard standard JJ cord-013178-li1x1m25 223 7 error error NN cord-013178-li1x1m25 223 8 ( ( -LRB- cord-013178-li1x1m25 223 9 SE SE NNP cord-013178-li1x1m25 223 10 ) ) -RRB- cord-013178-li1x1m25 223 11 . . . cord-013178-li1x1m25 224 1 For for IN cord-013178-li1x1m25 224 2 these these DT cord-013178-li1x1m25 224 3 homemade homemade JJ cord-013178-li1x1m25 224 4 PBMCs pbmc NNS cord-013178-li1x1m25 224 5 slides slide NNS cord-013178-li1x1m25 224 6 , , , cord-013178-li1x1m25 224 7 all all DT cord-013178-li1x1m25 224 8 slides slide NNS cord-013178-li1x1m25 224 9 were be VBD cord-013178-li1x1m25 224 10 made make VBN cord-013178-li1x1m25 224 11 from from IN cord-013178-li1x1m25 224 12 the the DT cord-013178-li1x1m25 224 13 same same JJ cord-013178-li1x1m25 224 14 batch batch NN cord-013178-li1x1m25 224 15 and and CC cord-013178-li1x1m25 224 16 contained contain VBD cord-013178-li1x1m25 224 17 the the DT cord-013178-li1x1m25 224 18 same same JJ cord-013178-li1x1m25 224 19 proportion proportion NN cord-013178-li1x1m25 224 20 of of IN cord-013178-li1x1m25 224 21 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 224 22 cells cell NNS cord-013178-li1x1m25 224 23 . . . cord-013178-li1x1m25 225 1 All all DT cord-013178-li1x1m25 225 2 wells well NNS cord-013178-li1x1m25 225 3 contained contain VBD cord-013178-li1x1m25 225 4 both both DT cord-013178-li1x1m25 225 5 negative negative JJ cord-013178-li1x1m25 225 6 and and CC cord-013178-li1x1m25 225 7 positive positive JJ cord-013178-li1x1m25 225 8 cells cell NNS cord-013178-li1x1m25 225 9 ( ( -LRB- cord-013178-li1x1m25 225 10 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 225 11 cells cell NNS cord-013178-li1x1m25 225 12 ) ) -RRB- cord-013178-li1x1m25 225 13 confirmed confirm VBN cord-013178-li1x1m25 225 14 by by IN cord-013178-li1x1m25 225 15 IFA IFA NNP cord-013178-li1x1m25 225 16 , , , cord-013178-li1x1m25 225 17 as as IN cord-013178-li1x1m25 225 18 previously previously RB cord-013178-li1x1m25 225 19 noted note VBN cord-013178-li1x1m25 225 20 in in IN cord-013178-li1x1m25 225 21 a a DT cord-013178-li1x1m25 225 22 similar similar JJ cord-013178-li1x1m25 225 23 study study NN cord-013178-li1x1m25 225 24 [ [ -LRB- cord-013178-li1x1m25 225 25 49 49 CD cord-013178-li1x1m25 225 26 ] ] -RRB- cord-013178-li1x1m25 225 27 . . . cord-013178-li1x1m25 226 1 In in IN cord-013178-li1x1m25 226 2 this this DT cord-013178-li1x1m25 226 3 study study NN cord-013178-li1x1m25 226 4 , , , cord-013178-li1x1m25 226 5 PCV2 pcv2 JJ cord-013178-li1x1m25 226 6 viral viral JJ cord-013178-li1x1m25 226 7 protein protein NN cord-013178-li1x1m25 226 8 ( ( -LRB- cord-013178-li1x1m25 226 9 ORF3 ORF3 NNP cord-013178-li1x1m25 226 10 protein protein NN cord-013178-li1x1m25 226 11 and and CC cord-013178-li1x1m25 226 12 capsid capsid NN cord-013178-li1x1m25 226 13 protein protein NN cord-013178-li1x1m25 226 14 ) ) -RRB- cord-013178-li1x1m25 226 15 locations location NNS cord-013178-li1x1m25 226 16 were be VBD cord-013178-li1x1m25 226 17 initially initially RB cord-013178-li1x1m25 226 18 assessed assess VBN cord-013178-li1x1m25 226 19 by by IN cord-013178-li1x1m25 226 20 IFA IFA NNP cord-013178-li1x1m25 226 21 . . . cord-013178-li1x1m25 227 1 The the DT cord-013178-li1x1m25 227 2 previous previous JJ cord-013178-li1x1m25 227 3 study study NN cord-013178-li1x1m25 227 4 indicated indicate VBD cord-013178-li1x1m25 227 5 that that IN cord-013178-li1x1m25 227 6 the the DT cord-013178-li1x1m25 227 7 mAb mAb NNS cord-013178-li1x1m25 227 8 1H3 1H3 NNP cord-013178-li1x1m25 227 9 bound bind VBD cord-013178-li1x1m25 227 10 the the DT cord-013178-li1x1m25 227 11 three three CD cord-013178-li1x1m25 227 12 minimal minimal JJ cord-013178-li1x1m25 227 13 linear linear JJ cord-013178-li1x1m25 227 14 epitopes epitope NNS cord-013178-li1x1m25 227 15 ( ( -LRB- cord-013178-li1x1m25 227 16 DPPLNP DPPLNP NNP cord-013178-li1x1m25 227 17 , , , cord-013178-li1x1m25 227 18 DPPLNPK DPPLNPK NNP cord-013178-li1x1m25 227 19 , , , cord-013178-li1x1m25 227 20 and and CC cord-013178-li1x1m25 227 21 LKDPPLKP LKDPPLKP NNP cord-013178-li1x1m25 227 22 ) ) -RRB- cord-013178-li1x1m25 227 23 , , , cord-013178-li1x1m25 227 24 which which WDT cord-013178-li1x1m25 227 25 were be VBD cord-013178-li1x1m25 227 26 located locate VBN cord-013178-li1x1m25 227 27 at at IN cord-013178-li1x1m25 227 28 carboxyl carboxyl NN cord-013178-li1x1m25 227 29 - - HYPH cord-013178-li1x1m25 227 30 terminus terminus NN cord-013178-li1x1m25 227 31 ( ( -LRB- cord-013178-li1x1m25 227 32 Cterminus Cterminus NNP cord-013178-li1x1m25 227 33 ) ) -RRB- cord-013178-li1x1m25 227 34 of of IN cord-013178-li1x1m25 227 35 the the DT cord-013178-li1x1m25 227 36 capsid capsid NN cord-013178-li1x1m25 227 37 proteins protein NNS cord-013178-li1x1m25 227 38 of of IN cord-013178-li1x1m25 227 39 PCV2b pcv2b NN cord-013178-li1x1m25 227 40 , , , cord-013178-li1x1m25 227 41 PCV2d PCV2d NNP cord-013178-li1x1m25 227 42 , , , cord-013178-li1x1m25 227 43 and and CC cord-013178-li1x1m25 227 44 PCV2a PCV2a NNP cord-013178-li1x1m25 227 45 , , , cord-013178-li1x1m25 227 46 respectively respectively RB cord-013178-li1x1m25 227 47 [ [ -LRB- cord-013178-li1x1m25 227 48 49 49 CD cord-013178-li1x1m25 227 49 ] ] -RRB- cord-013178-li1x1m25 227 50 . . . cord-013178-li1x1m25 228 1 Likewise likewise RB cord-013178-li1x1m25 228 2 , , , cord-013178-li1x1m25 228 3 the the DT cord-013178-li1x1m25 228 4 mAb mAb NNS cord-013178-li1x1m25 228 5 6B8 6b8 RB cord-013178-li1x1m25 228 6 bound bind VBD cord-013178-li1x1m25 228 7 two two CD cord-013178-li1x1m25 228 8 minimal minimal JJ cord-013178-li1x1m25 228 9 linear linear JJ cord-013178-li1x1m25 228 10 epitopes epitope NNS cord-013178-li1x1m25 228 11 ( ( -LRB- cord-013178-li1x1m25 228 12 KDPPLNP KDPPLNP NNP cord-013178-li1x1m25 228 13 and and CC cord-013178-li1x1m25 228 14 KDPPLNPK KDPPLNPK NNP cord-013178-li1x1m25 228 15 ) ) -RRB- cord-013178-li1x1m25 228 16 , , , cord-013178-li1x1m25 228 17 which which WDT cord-013178-li1x1m25 228 18 were be VBD cord-013178-li1x1m25 228 19 located locate VBN cord-013178-li1x1m25 228 20 at at IN cord-013178-li1x1m25 228 21 Cterminus Cterminus NNP cord-013178-li1x1m25 228 22 of of IN cord-013178-li1x1m25 228 23 the the DT cord-013178-li1x1m25 228 24 capsid capsid NN cord-013178-li1x1m25 228 25 proteins protein NNS cord-013178-li1x1m25 228 26 of of IN cord-013178-li1x1m25 228 27 PCV2b pcv2b NN cord-013178-li1x1m25 228 28 , , , cord-013178-li1x1m25 228 29 and and CC cord-013178-li1x1m25 228 30 PCV2d PCV2d NNS cord-013178-li1x1m25 228 31 , , , cord-013178-li1x1m25 228 32 respectively respectively RB cord-013178-li1x1m25 228 33 [ [ -LRB- cord-013178-li1x1m25 228 34 49 49 CD cord-013178-li1x1m25 228 35 ] ] -RRB- cord-013178-li1x1m25 228 36 . . . cord-013178-li1x1m25 229 1 of of IN cord-013178-li1x1m25 229 2 PBMCs pbmc NNS cord-013178-li1x1m25 229 3 were be VBD cord-013178-li1x1m25 229 4 positive positive JJ cord-013178-li1x1m25 229 5 for for IN cord-013178-li1x1m25 229 6 anti anti JJ cord-013178-li1x1m25 229 7 - - JJ cord-013178-li1x1m25 229 8 ORF3 ORF3 NNP cord-013178-li1x1m25 229 9 mAb mAb . cord-013178-li1x1m25 229 10 ( ( -LRB- cord-013178-li1x1m25 229 11 7D3 7d3 CD cord-013178-li1x1m25 229 12 ) ) -RRB- cord-013178-li1x1m25 229 13 staining staining NN cord-013178-li1x1m25 229 14 ( ( -LRB- cord-013178-li1x1m25 229 15 Figure figure NN cord-013178-li1x1m25 229 16 S3 S3 NNP cord-013178-li1x1m25 229 17 ) ) -RRB- cord-013178-li1x1m25 229 18 . . . cord-013178-li1x1m25 230 1 It -PRON- PRP cord-013178-li1x1m25 230 2 might may MD cord-013178-li1x1m25 230 3 be be VB cord-013178-li1x1m25 230 4 due due JJ cord-013178-li1x1m25 230 5 to to IN cord-013178-li1x1m25 230 6 different different JJ cord-013178-li1x1m25 230 7 abundance abundance NN cord-013178-li1x1m25 230 8 of of IN cord-013178-li1x1m25 230 9 the the DT cord-013178-li1x1m25 230 10 protein protein NN cord-013178-li1x1m25 230 11 , , , cord-013178-li1x1m25 230 12 the the DT cord-013178-li1x1m25 230 13 degradation degradation NN cord-013178-li1x1m25 230 14 of of IN cord-013178-li1x1m25 230 15 the the DT cord-013178-li1x1m25 230 16 protein protein NN cord-013178-li1x1m25 230 17 , , , cord-013178-li1x1m25 230 18 different different JJ cord-013178-li1x1m25 230 19 PCV2 pcv2 JJ cord-013178-li1x1m25 230 20 strains strain NNS cord-013178-li1x1m25 230 21 , , , cord-013178-li1x1m25 230 22 conformational conformational JJ cord-013178-li1x1m25 230 23 epitopes epitope NNS cord-013178-li1x1m25 230 24 , , , cord-013178-li1x1m25 230 25 or or CC cord-013178-li1x1m25 230 26 linear linear JJ cord-013178-li1x1m25 230 27 epitopes epitope NNS cord-013178-li1x1m25 230 28 , , , cord-013178-li1x1m25 230 29 which which WDT cord-013178-li1x1m25 230 30 interacted interact VBD cord-013178-li1x1m25 230 31 with with IN cord-013178-li1x1m25 230 32 the the DT cord-013178-li1x1m25 230 33 antibody antibody NN cord-013178-li1x1m25 230 34 . . . cord-013178-li1x1m25 231 1 Interestingly interestingly RB cord-013178-li1x1m25 231 2 , , , cord-013178-li1x1m25 231 3 the the DT cord-013178-li1x1m25 231 4 anti anti JJ cord-013178-li1x1m25 231 5 - - JJ cord-013178-li1x1m25 231 6 VLP VLP NNP cord-013178-li1x1m25 231 7 of of IN cord-013178-li1x1m25 231 8 PCV2 pcv2 JJ cord-013178-li1x1m25 231 9 rabbit rabbit NN cord-013178-li1x1m25 231 10 serum serum NN cord-013178-li1x1m25 231 11 produced produce VBD cord-013178-li1x1m25 231 12 cytoplasmic cytoplasmic JJ cord-013178-li1x1m25 231 13 staining staining NN cord-013178-li1x1m25 231 14 in in IN cord-013178-li1x1m25 231 15 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 231 16 PBMCs pbmc NNS cord-013178-li1x1m25 231 17 ( ( -LRB- cord-013178-li1x1m25 231 18 Figure figure NN cord-013178-li1x1m25 231 19 3B 3b NN cord-013178-li1x1m25 231 20 , , , cord-013178-li1x1m25 231 21 F F NNP cord-013178-li1x1m25 231 22 ) ) -RRB- cord-013178-li1x1m25 231 23 . . . cord-013178-li1x1m25 232 1 When when WRB cord-013178-li1x1m25 232 2 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 232 3 PBMCs pbmc NNS cord-013178-li1x1m25 232 4 were be VBD cord-013178-li1x1m25 232 5 co co JJ cord-013178-li1x1m25 232 6 - - JJ cord-013178-li1x1m25 232 7 stained stain VBN cord-013178-li1x1m25 232 8 with with IN cord-013178-li1x1m25 232 9 anti anti JJ cord-013178-li1x1m25 232 10 - - JJ cord-013178-li1x1m25 232 11 VLP VLP NNP cord-013178-li1x1m25 232 12 of of IN cord-013178-li1x1m25 232 13 PCV2 pcv2 JJ cord-013178-li1x1m25 232 14 rabbit rabbit NN cord-013178-li1x1m25 232 15 serum serum NN cord-013178-li1x1m25 232 16 and and CC cord-013178-li1x1m25 232 17 anti anti JJ cord-013178-li1x1m25 232 18 - - JJ cord-013178-li1x1m25 232 19 capsid capsid JJ cord-013178-li1x1m25 232 20 peptide peptide JJ cord-013178-li1x1m25 232 21 mAbs mAbs NNP cord-013178-li1x1m25 232 22 ( ( -LRB- cord-013178-li1x1m25 232 23 6B8 6b8 CD cord-013178-li1x1m25 232 24 ) ) -RRB- cord-013178-li1x1m25 232 25 , , , cord-013178-li1x1m25 232 26 some some DT cord-013178-li1x1m25 232 27 VLP VLP NNP cord-013178-li1x1m25 232 28 / / SYM cord-013178-li1x1m25 232 29 capsid capsid NN cord-013178-li1x1m25 232 30 peptide peptide JJ cord-013178-li1x1m25 232 31 dual dual JJ cord-013178-li1x1m25 232 32 - - HYPH cord-013178-li1x1m25 232 33 positive positive JJ cord-013178-li1x1m25 232 34 cells cell NNS cord-013178-li1x1m25 232 35 were be VBD cord-013178-li1x1m25 232 36 shown show VBN cord-013178-li1x1m25 232 37 ( ( -LRB- cord-013178-li1x1m25 232 38 Figure Figure NNP cord-013178-li1x1m25 232 39 3D 3d NN cord-013178-li1x1m25 232 40 ) ) -RRB- cord-013178-li1x1m25 232 41 . . . cord-013178-li1x1m25 233 1 The the DT cord-013178-li1x1m25 233 2 overlap overlap NN cord-013178-li1x1m25 233 3 between between IN cord-013178-li1x1m25 233 4 the the DT cord-013178-li1x1m25 233 5 anti anti JJ cord-013178-li1x1m25 233 6 - - JJ cord-013178-li1x1m25 233 7 VLP vlp JJ cord-013178-li1x1m25 233 8 rabbit rabbit NN cord-013178-li1x1m25 233 9 serum serum NN cord-013178-li1x1m25 233 10 staining staining NN cord-013178-li1x1m25 233 11 and and CC cord-013178-li1x1m25 233 12 the the DT cord-013178-li1x1m25 233 13 anti anti JJ cord-013178-li1x1m25 233 14 - - NNP cord-013178-li1x1m25 233 15 ORF3 ORF3 NNP cord-013178-li1x1m25 233 16 mAb mAb . cord-013178-li1x1m25 233 17 ( ( -LRB- cord-013178-li1x1m25 233 18 7D3 7d3 CD cord-013178-li1x1m25 233 19 ) ) -RRB- cord-013178-li1x1m25 233 20 staining staining NN cord-013178-li1x1m25 233 21 is be VBZ cord-013178-li1x1m25 233 22 presented present VBN cord-013178-li1x1m25 233 23 in in IN cord-013178-li1x1m25 233 24 Figure figure NN cord-013178-li1x1m25 233 25 3H 3H NNP cord-013178-li1x1m25 233 26 , , , cord-013178-li1x1m25 233 27 and and CC cord-013178-li1x1m25 233 28 the the DT cord-013178-li1x1m25 233 29 anti anti JJ cord-013178-li1x1m25 233 30 - - JJ cord-013178-li1x1m25 233 31 ORF3 orf3 JJ cord-013178-li1x1m25 233 32 polyclonal polyclonal JJ cord-013178-li1x1m25 233 33 antibody antibody NN cord-013178-li1x1m25 233 34 and and CC cord-013178-li1x1m25 233 35 anti anti JJ cord-013178-li1x1m25 233 36 - - JJ cord-013178-li1x1m25 233 37 capsid capsid JJ cord-013178-li1x1m25 233 38 mAb mAb NNS cord-013178-li1x1m25 233 39 ( ( -LRB- cord-013178-li1x1m25 233 40 36A9 36a9 CD cord-013178-li1x1m25 233 41 ) ) -RRB- cord-013178-li1x1m25 233 42 overlapped overlap VBD cord-013178-li1x1m25 233 43 the the DT cord-013178-li1x1m25 233 44 same same JJ cord-013178-li1x1m25 233 45 in in IN cord-013178-li1x1m25 233 46 Figure figure NN cord-013178-li1x1m25 233 47 3P 3p CD cord-013178-li1x1m25 233 48 ; ; : cord-013178-li1x1m25 233 49 however however RB cord-013178-li1x1m25 233 50 , , , cord-013178-li1x1m25 233 51 the the DT cord-013178-li1x1m25 233 52 polyclonal polyclonal JJ cord-013178-li1x1m25 233 53 antibody antibody NN cord-013178-li1x1m25 233 54 staining staining NN cord-013178-li1x1m25 233 55 was be VBD cord-013178-li1x1m25 233 56 brighter bright JJR cord-013178-li1x1m25 233 57 than than IN cord-013178-li1x1m25 233 58 the the DT cord-013178-li1x1m25 233 59 mAb mab NN cord-013178-li1x1m25 233 60 staining staining NN cord-013178-li1x1m25 233 61 . . . cord-013178-li1x1m25 234 1 This this DT cord-013178-li1x1m25 234 2 result result NN cord-013178-li1x1m25 234 3 shows show VBZ cord-013178-li1x1m25 234 4 ORF3 ORF3 NNP cord-013178-li1x1m25 234 5 protein protein NN cord-013178-li1x1m25 234 6 overlaps overlap VBZ cord-013178-li1x1m25 234 7 the the DT cord-013178-li1x1m25 234 8 capsid capsid NN cord-013178-li1x1m25 234 9 protein protein NN cord-013178-li1x1m25 234 10 ( ( -LRB- cord-013178-li1x1m25 234 11 or or CC cord-013178-li1x1m25 234 12 VLP VLP NNP cord-013178-li1x1m25 234 13 ) ) -RRB- cord-013178-li1x1m25 234 14 of of IN cord-013178-li1x1m25 234 15 PCV2 PCV2 NNP cord-013178-li1x1m25 234 16 ( ( -LRB- cord-013178-li1x1m25 234 17 Figure figure NN cord-013178-li1x1m25 234 18 3H 3h NN cord-013178-li1x1m25 234 19 , , , cord-013178-li1x1m25 234 20 P p NN cord-013178-li1x1m25 234 21 ) ) -RRB- cord-013178-li1x1m25 234 22 . . . cord-013178-li1x1m25 235 1 Both both DT cord-013178-li1x1m25 235 2 the the DT cord-013178-li1x1m25 235 3 capsid capsid NN cord-013178-li1x1m25 235 4 proteins protein NNS cord-013178-li1x1m25 235 5 and and CC cord-013178-li1x1m25 235 6 the the DT cord-013178-li1x1m25 235 7 ORF3 ORF3 NNP cord-013178-li1x1m25 235 8 protein protein NN cord-013178-li1x1m25 235 9 were be VBD cord-013178-li1x1m25 235 10 detected detect VBN cord-013178-li1x1m25 235 11 mainly mainly RB cord-013178-li1x1m25 235 12 in in IN cord-013178-li1x1m25 235 13 the the DT cord-013178-li1x1m25 235 14 cytoplasm cytoplasm NN cord-013178-li1x1m25 235 15 . . . cord-013178-li1x1m25 236 1 According accord VBG cord-013178-li1x1m25 236 2 to to IN cord-013178-li1x1m25 236 3 the the DT cord-013178-li1x1m25 236 4 previous previous JJ cord-013178-li1x1m25 236 5 study study NN cord-013178-li1x1m25 236 6 , , , cord-013178-li1x1m25 236 7 the the DT cord-013178-li1x1m25 236 8 N n CD cord-013178-li1x1m25 236 9 - - HYPH cord-013178-li1x1m25 236 10 terminal terminal JJ cord-013178-li1x1m25 236 11 half half NN cord-013178-li1x1m25 236 12 of of IN cord-013178-li1x1m25 236 13 ORF3 ORF3 NNP cord-013178-li1x1m25 236 14 protein protein NN cord-013178-li1x1m25 236 15 ( ( -LRB- cord-013178-li1x1m25 236 16 residues residue NNS cord-013178-li1x1m25 236 17 1 1 CD cord-013178-li1x1m25 236 18 - - SYM cord-013178-li1x1m25 236 19 53 53 CD cord-013178-li1x1m25 236 20 ) ) -RRB- cord-013178-li1x1m25 236 21 was be VBD cord-013178-li1x1m25 236 22 discovered discover VBN cord-013178-li1x1m25 236 23 in in IN cord-013178-li1x1m25 236 24 the the DT cord-013178-li1x1m25 236 25 cytoplasm cytoplasm NN cord-013178-li1x1m25 236 26 , , , cord-013178-li1x1m25 236 27 and and CC cord-013178-li1x1m25 236 28 the the DT cord-013178-li1x1m25 236 29 C C NNP cord-013178-li1x1m25 236 30 - - HYPH cord-013178-li1x1m25 236 31 terminal terminal NN cord-013178-li1x1m25 236 32 half half NN cord-013178-li1x1m25 236 33 of of IN cord-013178-li1x1m25 236 34 ORF3 ORF3 NNP cord-013178-li1x1m25 236 35 protein protein NN cord-013178-li1x1m25 236 36 ( ( -LRB- cord-013178-li1x1m25 236 37 residues residue NNS cord-013178-li1x1m25 236 38 53 53 CD cord-013178-li1x1m25 236 39 - - SYM cord-013178-li1x1m25 236 40 104 104 CD cord-013178-li1x1m25 236 41 ) ) -RRB- cord-013178-li1x1m25 236 42 was be VBD cord-013178-li1x1m25 236 43 majorly majorly RB cord-013178-li1x1m25 236 44 located locate VBN cord-013178-li1x1m25 236 45 in in IN cord-013178-li1x1m25 236 46 the the DT cord-013178-li1x1m25 236 47 nucleus nucleus NN cord-013178-li1x1m25 236 48 [ [ -LRB- cord-013178-li1x1m25 236 49 50 50 CD cord-013178-li1x1m25 236 50 ] ] -RRB- cord-013178-li1x1m25 236 51 . . . cord-013178-li1x1m25 237 1 The the DT cord-013178-li1x1m25 237 2 medial medial JJ cord-013178-li1x1m25 237 3 region region NN cord-013178-li1x1m25 237 4 of of IN cord-013178-li1x1m25 237 5 ORF3 ORF3 NNP cord-013178-li1x1m25 237 6 protein protein NN cord-013178-li1x1m25 237 7 ( ( -LRB- cord-013178-li1x1m25 237 8 residues residue NNS cord-013178-li1x1m25 237 9 35 35 CD cord-013178-li1x1m25 237 10 - - SYM cord-013178-li1x1m25 237 11 66 66 CD cord-013178-li1x1m25 237 12 ) ) -RRB- cord-013178-li1x1m25 237 13 might may MD cord-013178-li1x1m25 237 14 be be VB cord-013178-li1x1m25 237 15 primarily primarily RB cord-013178-li1x1m25 237 16 located locate VBN cord-013178-li1x1m25 237 17 in in IN cord-013178-li1x1m25 237 18 the the DT cord-013178-li1x1m25 237 19 cytoplasm cytoplasm NN cord-013178-li1x1m25 237 20 in in IN cord-013178-li1x1m25 237 21 this this DT cord-013178-li1x1m25 237 22 study study NN cord-013178-li1x1m25 237 23 . . . cord-013178-li1x1m25 238 1 of of IN cord-013178-li1x1m25 238 2 PCV2 PCV2 NNP cord-013178-li1x1m25 239 1 ( ( -LRB- cord-013178-li1x1m25 239 2 Figure figure NN cord-013178-li1x1m25 239 3 3H 3h NN cord-013178-li1x1m25 239 4 , , , cord-013178-li1x1m25 239 5 P p NN cord-013178-li1x1m25 239 6 ) ) -RRB- cord-013178-li1x1m25 239 7 . . . cord-013178-li1x1m25 240 1 Both both DT cord-013178-li1x1m25 240 2 the the DT cord-013178-li1x1m25 240 3 capsid capsid NN cord-013178-li1x1m25 240 4 proteins protein NNS cord-013178-li1x1m25 240 5 and and CC cord-013178-li1x1m25 240 6 the the DT cord-013178-li1x1m25 240 7 ORF3 ORF3 NNP cord-013178-li1x1m25 240 8 protein protein NN cord-013178-li1x1m25 240 9 were be VBD cord-013178-li1x1m25 240 10 detected detect VBN cord-013178-li1x1m25 240 11 mainly mainly RB cord-013178-li1x1m25 240 12 in in IN cord-013178-li1x1m25 240 13 the the DT cord-013178-li1x1m25 240 14 cytoplasm cytoplasm NN cord-013178-li1x1m25 240 15 . . . cord-013178-li1x1m25 241 1 According accord VBG cord-013178-li1x1m25 241 2 to to IN cord-013178-li1x1m25 241 3 the the DT cord-013178-li1x1m25 241 4 previous previous JJ cord-013178-li1x1m25 241 5 study study NN cord-013178-li1x1m25 241 6 , , , cord-013178-li1x1m25 241 7 the the DT cord-013178-li1x1m25 241 8 N n CD cord-013178-li1x1m25 241 9 - - HYPH cord-013178-li1x1m25 241 10 terminal terminal JJ cord-013178-li1x1m25 241 11 half half NN cord-013178-li1x1m25 241 12 of of IN cord-013178-li1x1m25 241 13 ORF3 ORF3 NNP cord-013178-li1x1m25 241 14 protein protein NN cord-013178-li1x1m25 241 15 ( ( -LRB- cord-013178-li1x1m25 241 16 residues residue NNS cord-013178-li1x1m25 241 17 1 1 CD cord-013178-li1x1m25 241 18 - - SYM cord-013178-li1x1m25 241 19 53 53 CD cord-013178-li1x1m25 241 20 ) ) -RRB- cord-013178-li1x1m25 241 21 was be VBD cord-013178-li1x1m25 241 22 discovered discover VBN cord-013178-li1x1m25 241 23 in in IN cord-013178-li1x1m25 241 24 the the DT cord-013178-li1x1m25 241 25 cytoplasm cytoplasm NN cord-013178-li1x1m25 241 26 , , , cord-013178-li1x1m25 241 27 and and CC cord-013178-li1x1m25 241 28 the the DT cord-013178-li1x1m25 241 29 C C NNP cord-013178-li1x1m25 241 30 - - HYPH cord-013178-li1x1m25 241 31 terminal terminal NN cord-013178-li1x1m25 241 32 half half NN cord-013178-li1x1m25 241 33 of of IN cord-013178-li1x1m25 241 34 ORF3 ORF3 NNP cord-013178-li1x1m25 241 35 protein protein NN cord-013178-li1x1m25 241 36 ( ( -LRB- cord-013178-li1x1m25 241 37 residues residue NNS cord-013178-li1x1m25 241 38 53 53 CD cord-013178-li1x1m25 241 39 - - SYM cord-013178-li1x1m25 241 40 104 104 CD cord-013178-li1x1m25 241 41 ) ) -RRB- cord-013178-li1x1m25 241 42 was be VBD cord-013178-li1x1m25 241 43 majorly majorly RB cord-013178-li1x1m25 241 44 located locate VBN cord-013178-li1x1m25 241 45 in in IN cord-013178-li1x1m25 241 46 the the DT cord-013178-li1x1m25 241 47 nucleus nucleus NN cord-013178-li1x1m25 241 48 [ [ -LRB- cord-013178-li1x1m25 241 49 50 50 CD cord-013178-li1x1m25 241 50 ] ] -RRB- cord-013178-li1x1m25 241 51 . . . cord-013178-li1x1m25 242 1 The the DT cord-013178-li1x1m25 242 2 medial medial JJ cord-013178-li1x1m25 242 3 region region NN cord-013178-li1x1m25 242 4 of of IN cord-013178-li1x1m25 242 5 ORF3 ORF3 NNP cord-013178-li1x1m25 242 6 protein protein NN cord-013178-li1x1m25 242 7 ( ( -LRB- cord-013178-li1x1m25 242 8 residues residue NNS cord-013178-li1x1m25 242 9 35 35 CD cord-013178-li1x1m25 242 10 - - SYM cord-013178-li1x1m25 242 11 66 66 CD cord-013178-li1x1m25 242 12 ) ) -RRB- cord-013178-li1x1m25 242 13 might may MD cord-013178-li1x1m25 242 14 be be VB cord-013178-li1x1m25 242 15 primarily primarily RB cord-013178-li1x1m25 242 16 located locate VBN cord-013178-li1x1m25 242 17 in in IN cord-013178-li1x1m25 242 18 the the DT cord-013178-li1x1m25 242 19 cytoplasm cytoplasm NN cord-013178-li1x1m25 242 20 in in IN cord-013178-li1x1m25 242 21 this this DT cord-013178-li1x1m25 242 22 study study NN cord-013178-li1x1m25 242 23 . . . cord-013178-li1x1m25 243 1 A a DT cord-013178-li1x1m25 243 2 previous previous JJ cord-013178-li1x1m25 243 3 study study NN cord-013178-li1x1m25 243 4 suggested suggest VBD cord-013178-li1x1m25 243 5 that that IN cord-013178-li1x1m25 243 6 ORF3 ORF3 NNP cord-013178-li1x1m25 243 7 protein protein NN cord-013178-li1x1m25 243 8 led lead VBD cord-013178-li1x1m25 243 9 to to IN cord-013178-li1x1m25 243 10 increased increase VBN cord-013178-li1x1m25 243 11 p53 p53 NN cord-013178-li1x1m25 243 12 protein protein NN cord-013178-li1x1m25 243 13 levels level NNS cord-013178-li1x1m25 243 14 and and CC cord-013178-li1x1m25 243 15 apoptosis apoptosis NN cord-013178-li1x1m25 243 16 of of IN cord-013178-li1x1m25 243 17 the the DT cord-013178-li1x1m25 243 18 infected infected JJ cord-013178-li1x1m25 243 19 cells cell NNS cord-013178-li1x1m25 243 20 [ [ -LRB- cord-013178-li1x1m25 243 21 42 42 CD cord-013178-li1x1m25 243 22 ] ] -RRB- cord-013178-li1x1m25 243 23 . . . cord-013178-li1x1m25 244 1 To to TO cord-013178-li1x1m25 244 2 identify identify VB cord-013178-li1x1m25 244 3 the the DT cord-013178-li1x1m25 244 4 subcellular subcellular JJ cord-013178-li1x1m25 244 5 location location NN cord-013178-li1x1m25 244 6 of of IN cord-013178-li1x1m25 244 7 the the DT cord-013178-li1x1m25 244 8 ORF3 ORF3 NNP cord-013178-li1x1m25 244 9 protein protein NN cord-013178-li1x1m25 244 10 in in IN cord-013178-li1x1m25 244 11 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 244 12 PBMCs PBMCs NNP cord-013178-li1x1m25 244 13 , , , cord-013178-li1x1m25 244 14 anti anti JJ cord-013178-li1x1m25 244 15 - - JJ cord-013178-li1x1m25 244 16 capsid capsid JJ cord-013178-li1x1m25 244 17 , , , cord-013178-li1x1m25 244 18 anti anti NNP cord-013178-li1x1m25 244 19 - - NNP cord-013178-li1x1m25 244 20 ORF3 ORF3 NNP cord-013178-li1x1m25 244 21 , , , cord-013178-li1x1m25 244 22 and and CC cord-013178-li1x1m25 244 23 anti anti JJ cord-013178-li1x1m25 244 24 - - JJ cord-013178-li1x1m25 244 25 p53 p53 JJ cord-013178-li1x1m25 244 26 antibodies antibody NNS cord-013178-li1x1m25 244 27 were be VBD cord-013178-li1x1m25 244 28 used use VBN cord-013178-li1x1m25 244 29 . . . cord-013178-li1x1m25 245 1 The the DT cord-013178-li1x1m25 245 2 p53 p53 NN cord-013178-li1x1m25 245 3 protein protein NN cord-013178-li1x1m25 245 4 also also RB cord-013178-li1x1m25 245 5 colocalized colocalize VBD cord-013178-li1x1m25 245 6 with with IN cord-013178-li1x1m25 245 7 capsid capsid NN cord-013178-li1x1m25 245 8 peptide peptide NN cord-013178-li1x1m25 245 9 ( ( -LRB- cord-013178-li1x1m25 245 10 Figure figure NN cord-013178-li1x1m25 245 11 3L 3l CD cord-013178-li1x1m25 245 12 ) ) -RRB- cord-013178-li1x1m25 245 13 , , , cord-013178-li1x1m25 245 14 and and CC cord-013178-li1x1m25 245 15 the the DT cord-013178-li1x1m25 245 16 ORF3 ORF3 NNP cord-013178-li1x1m25 245 17 peptide peptide NN cord-013178-li1x1m25 245 18 ( ( -LRB- cord-013178-li1x1m25 245 19 Figure figure NN cord-013178-li1x1m25 245 20 3X 3x CD cord-013178-li1x1m25 245 21 ) ) -RRB- cord-013178-li1x1m25 245 22 . . . cord-013178-li1x1m25 246 1 The the DT cord-013178-li1x1m25 246 2 p53 p53 NN cord-013178-li1x1m25 246 3 protein protein NN cord-013178-li1x1m25 246 4 was be VBD cord-013178-li1x1m25 246 5 mainly mainly RB cord-013178-li1x1m25 246 6 distributed distribute VBN cord-013178-li1x1m25 246 7 in in IN cord-013178-li1x1m25 246 8 the the DT cord-013178-li1x1m25 246 9 cytoplasm cytoplasm NN cord-013178-li1x1m25 246 10 and and CC cord-013178-li1x1m25 246 11 around around IN cord-013178-li1x1m25 246 12 the the DT cord-013178-li1x1m25 246 13 nucleus nucleus NN cord-013178-li1x1m25 246 14 ( ( -LRB- cord-013178-li1x1m25 246 15 Figure Figure NNP cord-013178-li1x1m25 246 16 3J 3j NN cord-013178-li1x1m25 246 17 , , , cord-013178-li1x1m25 246 18 V v NN cord-013178-li1x1m25 246 19 ) ) -RRB- cord-013178-li1x1m25 246 20 . . . cord-013178-li1x1m25 247 1 The the DT cord-013178-li1x1m25 247 2 ORF3 ORF3 NNP cord-013178-li1x1m25 247 3 protein protein NN cord-013178-li1x1m25 247 4 , , , cord-013178-li1x1m25 247 5 as as IN cord-013178-li1x1m25 247 6 detected detect VBN cord-013178-li1x1m25 247 7 with with IN cord-013178-li1x1m25 247 8 mAb mAb NNP cord-013178-li1x1m25 247 9 7D3 7d3 CD cord-013178-li1x1m25 247 10 , , , cord-013178-li1x1m25 247 11 seemed seem VBD cord-013178-li1x1m25 247 12 to to TO cord-013178-li1x1m25 247 13 colocalize colocalize VB cord-013178-li1x1m25 247 14 quite quite RB cord-013178-li1x1m25 247 15 nicely nicely RB cord-013178-li1x1m25 247 16 with with IN cord-013178-li1x1m25 247 17 the the DT cord-013178-li1x1m25 247 18 p53 p53 NN cord-013178-li1x1m25 247 19 protein protein NN cord-013178-li1x1m25 247 20 ( ( -LRB- cord-013178-li1x1m25 247 21 Figure figure NN cord-013178-li1x1m25 247 22 3X 3x CD cord-013178-li1x1m25 247 23 ) ) -RRB- cord-013178-li1x1m25 247 24 . . . cord-013178-li1x1m25 248 1 The the DT cord-013178-li1x1m25 248 2 p53 p53 NN cord-013178-li1x1m25 248 3 / / SYM cord-013178-li1x1m25 248 4 ORF3 ORF3 NNP cord-013178-li1x1m25 248 5 dual dual JJ cord-013178-li1x1m25 248 6 - - HYPH cord-013178-li1x1m25 248 7 positive positive JJ cord-013178-li1x1m25 248 8 cell cell NN cord-013178-li1x1m25 248 9 presented present VBD cord-013178-li1x1m25 248 10 a a DT cord-013178-li1x1m25 248 11 segmented segmented JJ cord-013178-li1x1m25 248 12 nucleus nucleus NN cord-013178-li1x1m25 248 13 ( ( -LRB- cord-013178-li1x1m25 248 14 Figure Figure NNP cord-013178-li1x1m25 248 15 3W 3w CD cord-013178-li1x1m25 248 16 , , , cord-013178-li1x1m25 248 17 Y Y NNP cord-013178-li1x1m25 248 18 ) ) -RRB- cord-013178-li1x1m25 248 19 . . . cord-013178-li1x1m25 249 1 The the DT cord-013178-li1x1m25 249 2 nuclei nucleus NNS cord-013178-li1x1m25 249 3 of of IN cord-013178-li1x1m25 249 4 negative negative JJ cord-013178-li1x1m25 249 5 cells cell NNS cord-013178-li1x1m25 249 6 were be VBD cord-013178-li1x1m25 249 7 round round JJ cord-013178-li1x1m25 249 8 or or CC cord-013178-li1x1m25 249 9 oval oval JJ cord-013178-li1x1m25 249 10 , , , cord-013178-li1x1m25 249 11 and and CC cord-013178-li1x1m25 249 12 nuclear nuclear JJ cord-013178-li1x1m25 249 13 segmentation segmentation NN cord-013178-li1x1m25 249 14 was be VBD cord-013178-li1x1m25 249 15 very very RB cord-013178-li1x1m25 249 16 rare rare JJ cord-013178-li1x1m25 249 17 in in IN cord-013178-li1x1m25 249 18 negative negative JJ cord-013178-li1x1m25 249 19 cells cell NNS cord-013178-li1x1m25 249 20 ( ( -LRB- cord-013178-li1x1m25 249 21 Figure figure NN cord-013178-li1x1m25 249 22 3Y 3y NN cord-013178-li1x1m25 249 23 ) ) -RRB- cord-013178-li1x1m25 249 24 . . . cord-013178-li1x1m25 250 1 The the DT cord-013178-li1x1m25 250 2 apoptosis apoptosis NN cord-013178-li1x1m25 250 3 in in IN cord-013178-li1x1m25 250 4 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 250 5 PBMCs pbmc NNS cord-013178-li1x1m25 250 6 was be VBD cord-013178-li1x1m25 250 7 observed observe VBN cord-013178-li1x1m25 250 8 by by IN cord-013178-li1x1m25 250 9 using use VBG cord-013178-li1x1m25 250 10 a a DT cord-013178-li1x1m25 250 11 terminal terminal JJ cord-013178-li1x1m25 250 12 deoxynucleotidyl deoxynucleotidyl NNP cord-013178-li1x1m25 250 13 transferase transferase NNP cord-013178-li1x1m25 250 14 dUTP dUTP NNP cord-013178-li1x1m25 250 15 nick nick NNP cord-013178-li1x1m25 250 16 end end NNP cord-013178-li1x1m25 250 17 labeling label VBG cord-013178-li1x1m25 250 18 ( ( -LRB- cord-013178-li1x1m25 250 19 TUNEL TUNEL NNP cord-013178-li1x1m25 250 20 ) ) -RRB- cord-013178-li1x1m25 250 21 assay assay NN cord-013178-li1x1m25 250 22 ( ( -LRB- cord-013178-li1x1m25 250 23 Figures figure NNS cord-013178-li1x1m25 250 24 4 4 CD cord-013178-li1x1m25 250 25 and and CC cord-013178-li1x1m25 250 26 S4 S4 NNP cord-013178-li1x1m25 250 27 ) ) -RRB- cord-013178-li1x1m25 250 28 . . . cord-013178-li1x1m25 251 1 The the DT cord-013178-li1x1m25 251 2 data datum NNS cord-013178-li1x1m25 251 3 show show VBP cord-013178-li1x1m25 251 4 that that IN cord-013178-li1x1m25 251 5 3 3 CD cord-013178-li1x1m25 251 6 - - SYM cord-013178-li1x1m25 251 7 4 4 CD cord-013178-li1x1m25 251 8 % % NN cord-013178-li1x1m25 251 9 PBMCs pbmc NNS cord-013178-li1x1m25 251 10 were be VBD cord-013178-li1x1m25 251 11 positive positive JJ cord-013178-li1x1m25 251 12 for for IN cord-013178-li1x1m25 251 13 TUNEL TUNEL NNP cord-013178-li1x1m25 251 14 staining staining NN cord-013178-li1x1m25 251 15 . . . cord-013178-li1x1m25 252 1 It -PRON- PRP cord-013178-li1x1m25 252 2 is be VBZ cord-013178-li1x1m25 252 3 very very RB cord-013178-li1x1m25 252 4 similar similar JJ cord-013178-li1x1m25 252 5 to to IN cord-013178-li1x1m25 252 6 the the DT cord-013178-li1x1m25 252 7 percentage percentage NN cord-013178-li1x1m25 252 8 of of IN cord-013178-li1x1m25 252 9 anti anti JJ cord-013178-li1x1m25 252 10 - - JJ cord-013178-li1x1m25 252 11 ORF3 ORF3 NNP cord-013178-li1x1m25 252 12 mAb mAb . cord-013178-li1x1m25 252 13 ( ( -LRB- cord-013178-li1x1m25 252 14 7D3 7d3 CD cord-013178-li1x1m25 252 15 ) ) -RRB- cord-013178-li1x1m25 252 16 staining staining NN cord-013178-li1x1m25 252 17 . . . cord-013178-li1x1m25 253 1 However however RB cord-013178-li1x1m25 253 2 , , , cord-013178-li1x1m25 253 3 these these DT cord-013178-li1x1m25 253 4 similar similar JJ cord-013178-li1x1m25 253 5 percentages percentage NNS cord-013178-li1x1m25 253 6 are be VBP cord-013178-li1x1m25 253 7 not not RB cord-013178-li1x1m25 253 8 sufficient sufficient JJ cord-013178-li1x1m25 253 9 proof proof NN cord-013178-li1x1m25 253 10 to to TO cord-013178-li1x1m25 253 11 show show VB cord-013178-li1x1m25 253 12 that that IN cord-013178-li1x1m25 253 13 the the DT cord-013178-li1x1m25 253 14 ORF3 ORF3 NNP cord-013178-li1x1m25 253 15 protein protein NN cord-013178-li1x1m25 253 16 of of IN cord-013178-li1x1m25 253 17 PCV2 PCV2 NNP cord-013178-li1x1m25 253 18 causes cause VBZ cord-013178-li1x1m25 253 19 DNA dna NN cord-013178-li1x1m25 253 20 damage damage NN cord-013178-li1x1m25 253 21 . . . cord-013178-li1x1m25 254 1 A a DT cord-013178-li1x1m25 254 2 more more RBR cord-013178-li1x1m25 254 3 careful careful JJ cord-013178-li1x1m25 254 4 analysis analysis NN cord-013178-li1x1m25 254 5 would would MD cord-013178-li1x1m25 254 6 be be VB cord-013178-li1x1m25 254 7 needed need VBN cord-013178-li1x1m25 254 8 to to TO cord-013178-li1x1m25 254 9 reveal reveal VB cord-013178-li1x1m25 254 10 whether whether IN cord-013178-li1x1m25 254 11 PCV2 pcv2 JJ cord-013178-li1x1m25 254 12 infection infection NN cord-013178-li1x1m25 254 13 causes cause VBZ cord-013178-li1x1m25 254 14 DNA dna NN cord-013178-li1x1m25 254 15 damage damage NN cord-013178-li1x1m25 254 16 . . . cord-013178-li1x1m25 255 1 Unfortunately unfortunately RB cord-013178-li1x1m25 255 2 , , , cord-013178-li1x1m25 255 3 it -PRON- PRP cord-013178-li1x1m25 255 4 was be VBD cord-013178-li1x1m25 255 5 not not RB cord-013178-li1x1m25 255 6 easy easy JJ cord-013178-li1x1m25 255 7 to to TO cord-013178-li1x1m25 255 8 label label VB cord-013178-li1x1m25 255 9 both both CC cord-013178-li1x1m25 255 10 the the DT cord-013178-li1x1m25 255 11 ORF3 ORF3 NNP cord-013178-li1x1m25 255 12 protein protein NN cord-013178-li1x1m25 255 13 and and CC cord-013178-li1x1m25 255 14 the the DT cord-013178-li1x1m25 255 15 signals signal NNS cord-013178-li1x1m25 255 16 of of IN cord-013178-li1x1m25 255 17 DNA dna NN cord-013178-li1x1m25 255 18 breakage breakage NN cord-013178-li1x1m25 255 19 simultaneously simultaneously RB cord-013178-li1x1m25 255 20 by by IN cord-013178-li1x1m25 255 21 IFA IFA NNP cord-013178-li1x1m25 255 22 and and CC cord-013178-li1x1m25 255 23 TUNEL TUNEL NNP cord-013178-li1x1m25 255 24 assay assay NN cord-013178-li1x1m25 255 25 ( ( -LRB- cord-013178-li1x1m25 255 26 data datum NNS cord-013178-li1x1m25 255 27 not not RB cord-013178-li1x1m25 255 28 shown show VBN cord-013178-li1x1m25 255 29 ) ) -RRB- cord-013178-li1x1m25 255 30 . . . cord-013178-li1x1m25 256 1 Further further JJ cord-013178-li1x1m25 256 2 research research NN cord-013178-li1x1m25 256 3 would would MD cord-013178-li1x1m25 256 4 be be VB cord-013178-li1x1m25 256 5 needed need VBN cord-013178-li1x1m25 256 6 to to TO cord-013178-li1x1m25 256 7 determine determine VB cord-013178-li1x1m25 256 8 whether whether IN cord-013178-li1x1m25 256 9 the the DT cord-013178-li1x1m25 256 10 ORF3 ORF3 NNP cord-013178-li1x1m25 256 11 protein protein NN cord-013178-li1x1m25 256 12 colocalizes colocalize VBZ cord-013178-li1x1m25 256 13 with with IN cord-013178-li1x1m25 256 14 the the DT cord-013178-li1x1m25 256 15 signals signal NNS cord-013178-li1x1m25 256 16 of of IN cord-013178-li1x1m25 256 17 TUNEL TUNEL NNP cord-013178-li1x1m25 256 18 . . . cord-013178-li1x1m25 257 1 A a DT cord-013178-li1x1m25 257 2 previous previous JJ cord-013178-li1x1m25 257 3 study study NN cord-013178-li1x1m25 257 4 suggested suggest VBD cord-013178-li1x1m25 257 5 that that IN cord-013178-li1x1m25 257 6 ORF3 ORF3 NNP cord-013178-li1x1m25 257 7 protein protein NN cord-013178-li1x1m25 257 8 led lead VBD cord-013178-li1x1m25 257 9 to to IN cord-013178-li1x1m25 257 10 increased increase VBN cord-013178-li1x1m25 257 11 p53 p53 NN cord-013178-li1x1m25 257 12 protein protein NN cord-013178-li1x1m25 257 13 levels level NNS cord-013178-li1x1m25 257 14 and and CC cord-013178-li1x1m25 257 15 apoptosis apoptosis NN cord-013178-li1x1m25 257 16 of of IN cord-013178-li1x1m25 257 17 the the DT cord-013178-li1x1m25 257 18 infected infected JJ cord-013178-li1x1m25 257 19 cells cell NNS cord-013178-li1x1m25 257 20 [ [ -LRB- cord-013178-li1x1m25 257 21 42 42 CD cord-013178-li1x1m25 257 22 ] ] -RRB- cord-013178-li1x1m25 257 23 . . . cord-013178-li1x1m25 258 1 To to TO cord-013178-li1x1m25 258 2 identify identify VB cord-013178-li1x1m25 258 3 the the DT cord-013178-li1x1m25 258 4 subcellular subcellular JJ cord-013178-li1x1m25 258 5 location location NN cord-013178-li1x1m25 258 6 of of IN cord-013178-li1x1m25 258 7 the the DT cord-013178-li1x1m25 258 8 ORF3 ORF3 NNP cord-013178-li1x1m25 258 9 protein protein NN cord-013178-li1x1m25 258 10 in in IN cord-013178-li1x1m25 258 11 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 258 12 PBMCs PBMCs NNP cord-013178-li1x1m25 258 13 , , , cord-013178-li1x1m25 258 14 anti anti JJ cord-013178-li1x1m25 258 15 - - JJ cord-013178-li1x1m25 258 16 capsid capsid JJ cord-013178-li1x1m25 258 17 , , , cord-013178-li1x1m25 258 18 anti anti NNP cord-013178-li1x1m25 258 19 - - NNP cord-013178-li1x1m25 258 20 ORF3 ORF3 NNP cord-013178-li1x1m25 258 21 , , , cord-013178-li1x1m25 258 22 and and CC cord-013178-li1x1m25 258 23 anti anti JJ cord-013178-li1x1m25 258 24 - - JJ cord-013178-li1x1m25 258 25 p53 p53 JJ cord-013178-li1x1m25 258 26 antibodies antibody NNS cord-013178-li1x1m25 258 27 were be VBD cord-013178-li1x1m25 258 28 used use VBN cord-013178-li1x1m25 258 29 . . . cord-013178-li1x1m25 259 1 The the DT cord-013178-li1x1m25 259 2 p53 p53 NN cord-013178-li1x1m25 259 3 protein protein NN cord-013178-li1x1m25 259 4 also also RB cord-013178-li1x1m25 259 5 colocalized colocalize VBD cord-013178-li1x1m25 259 6 with with IN cord-013178-li1x1m25 259 7 capsid capsid NN cord-013178-li1x1m25 259 8 peptide peptide NN cord-013178-li1x1m25 259 9 ( ( -LRB- cord-013178-li1x1m25 259 10 Figure figure NN cord-013178-li1x1m25 259 11 3L 3l CD cord-013178-li1x1m25 259 12 ) ) -RRB- cord-013178-li1x1m25 259 13 , , , cord-013178-li1x1m25 259 14 and and CC cord-013178-li1x1m25 259 15 the the DT cord-013178-li1x1m25 259 16 ORF3 ORF3 NNP cord-013178-li1x1m25 259 17 peptide peptide NN cord-013178-li1x1m25 259 18 ( ( -LRB- cord-013178-li1x1m25 259 19 Figure figure NN cord-013178-li1x1m25 259 20 3X 3x CD cord-013178-li1x1m25 259 21 ) ) -RRB- cord-013178-li1x1m25 259 22 . . . cord-013178-li1x1m25 260 1 The the DT cord-013178-li1x1m25 260 2 p53 p53 NN cord-013178-li1x1m25 260 3 protein protein NN cord-013178-li1x1m25 260 4 was be VBD cord-013178-li1x1m25 260 5 mainly mainly RB cord-013178-li1x1m25 260 6 distributed distribute VBN cord-013178-li1x1m25 260 7 in in IN cord-013178-li1x1m25 260 8 the the DT cord-013178-li1x1m25 260 9 cytoplasm cytoplasm NN cord-013178-li1x1m25 260 10 and and CC cord-013178-li1x1m25 260 11 around around IN cord-013178-li1x1m25 260 12 the the DT cord-013178-li1x1m25 260 13 nucleus nucleus NN cord-013178-li1x1m25 260 14 ( ( -LRB- cord-013178-li1x1m25 260 15 Figure Figure NNP cord-013178-li1x1m25 260 16 3J 3j NN cord-013178-li1x1m25 260 17 , , , cord-013178-li1x1m25 260 18 V v NN cord-013178-li1x1m25 260 19 ) ) -RRB- cord-013178-li1x1m25 260 20 . . . cord-013178-li1x1m25 261 1 The the DT cord-013178-li1x1m25 261 2 ORF3 ORF3 NNP cord-013178-li1x1m25 261 3 protein protein NN cord-013178-li1x1m25 261 4 , , , cord-013178-li1x1m25 261 5 as as IN cord-013178-li1x1m25 261 6 detected detect VBN cord-013178-li1x1m25 261 7 with with IN cord-013178-li1x1m25 261 8 mAb mAb NNP cord-013178-li1x1m25 261 9 7D3 7d3 CD cord-013178-li1x1m25 261 10 , , , cord-013178-li1x1m25 261 11 seemed seem VBD cord-013178-li1x1m25 261 12 to to TO cord-013178-li1x1m25 261 13 colocalize colocalize VB cord-013178-li1x1m25 261 14 quite quite RB cord-013178-li1x1m25 261 15 nicely nicely RB cord-013178-li1x1m25 261 16 with with IN cord-013178-li1x1m25 261 17 the the DT cord-013178-li1x1m25 261 18 p53 p53 NN cord-013178-li1x1m25 261 19 protein protein NN cord-013178-li1x1m25 261 20 ( ( -LRB- cord-013178-li1x1m25 261 21 Figure figure NN cord-013178-li1x1m25 261 22 3X 3x CD cord-013178-li1x1m25 261 23 ) ) -RRB- cord-013178-li1x1m25 261 24 . . . cord-013178-li1x1m25 262 1 The the DT cord-013178-li1x1m25 262 2 p53 p53 NN cord-013178-li1x1m25 262 3 / / SYM cord-013178-li1x1m25 262 4 ORF3 ORF3 NNP cord-013178-li1x1m25 262 5 dual dual JJ cord-013178-li1x1m25 262 6 - - HYPH cord-013178-li1x1m25 262 7 positive positive JJ cord-013178-li1x1m25 262 8 cell cell NN cord-013178-li1x1m25 262 9 presented present VBD cord-013178-li1x1m25 262 10 a a DT cord-013178-li1x1m25 262 11 segmented segmented JJ cord-013178-li1x1m25 262 12 nucleus nucleus NN cord-013178-li1x1m25 262 13 ( ( -LRB- cord-013178-li1x1m25 262 14 Figure Figure NNP cord-013178-li1x1m25 262 15 3W 3w CD cord-013178-li1x1m25 262 16 , , , cord-013178-li1x1m25 262 17 Y Y NNP cord-013178-li1x1m25 262 18 ) ) -RRB- cord-013178-li1x1m25 262 19 . . . cord-013178-li1x1m25 263 1 The the DT cord-013178-li1x1m25 263 2 nuclei nucleus NNS cord-013178-li1x1m25 263 3 of of IN cord-013178-li1x1m25 263 4 negative negative JJ cord-013178-li1x1m25 263 5 cells cell NNS cord-013178-li1x1m25 263 6 were be VBD cord-013178-li1x1m25 263 7 round round JJ cord-013178-li1x1m25 263 8 or or CC cord-013178-li1x1m25 263 9 oval oval JJ cord-013178-li1x1m25 263 10 , , , cord-013178-li1x1m25 263 11 and and CC cord-013178-li1x1m25 263 12 nuclear nuclear JJ cord-013178-li1x1m25 263 13 segmentation segmentation NN cord-013178-li1x1m25 263 14 was be VBD cord-013178-li1x1m25 263 15 very very RB cord-013178-li1x1m25 263 16 rare rare JJ cord-013178-li1x1m25 263 17 in in IN cord-013178-li1x1m25 263 18 negative negative JJ cord-013178-li1x1m25 263 19 cells cell NNS cord-013178-li1x1m25 263 20 ( ( -LRB- cord-013178-li1x1m25 263 21 Figure figure NN cord-013178-li1x1m25 263 22 3Y 3y NN cord-013178-li1x1m25 263 23 ) ) -RRB- cord-013178-li1x1m25 263 24 . . . cord-013178-li1x1m25 264 1 The the DT cord-013178-li1x1m25 264 2 apoptosis apoptosis NN cord-013178-li1x1m25 264 3 in in IN cord-013178-li1x1m25 264 4 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 264 5 PBMCs pbmc NNS cord-013178-li1x1m25 264 6 was be VBD cord-013178-li1x1m25 264 7 observed observe VBN cord-013178-li1x1m25 264 8 by by IN cord-013178-li1x1m25 264 9 using use VBG cord-013178-li1x1m25 264 10 a a DT cord-013178-li1x1m25 264 11 terminal terminal JJ cord-013178-li1x1m25 264 12 deoxynucleotidyl deoxynucleotidyl NNP cord-013178-li1x1m25 264 13 transferase transferase NNP cord-013178-li1x1m25 264 14 dUTP dUTP NNP cord-013178-li1x1m25 264 15 nick nick NNP cord-013178-li1x1m25 264 16 end end NNP cord-013178-li1x1m25 264 17 labeling label VBG cord-013178-li1x1m25 264 18 ( ( -LRB- cord-013178-li1x1m25 264 19 TUNEL TUNEL NNP cord-013178-li1x1m25 264 20 ) ) -RRB- cord-013178-li1x1m25 264 21 assay assay NN cord-013178-li1x1m25 264 22 ( ( -LRB- cord-013178-li1x1m25 264 23 Figure figure NN cord-013178-li1x1m25 264 24 4 4 CD cord-013178-li1x1m25 264 25 and and CC cord-013178-li1x1m25 264 26 Figure Figure NNP cord-013178-li1x1m25 264 27 S4 S4 NNP cord-013178-li1x1m25 264 28 ) ) -RRB- cord-013178-li1x1m25 264 29 . . . cord-013178-li1x1m25 265 1 The the DT cord-013178-li1x1m25 265 2 data datum NNS cord-013178-li1x1m25 265 3 show show VBP cord-013178-li1x1m25 265 4 that that IN cord-013178-li1x1m25 265 5 3 3 CD cord-013178-li1x1m25 265 6 - - SYM cord-013178-li1x1m25 265 7 4 4 CD cord-013178-li1x1m25 265 8 % % NN cord-013178-li1x1m25 265 9 PBMCs pbmc NNS cord-013178-li1x1m25 265 10 were be VBD cord-013178-li1x1m25 265 11 positive positive JJ cord-013178-li1x1m25 265 12 for for IN cord-013178-li1x1m25 265 13 TUNEL TUNEL NNP cord-013178-li1x1m25 265 14 staining staining NN cord-013178-li1x1m25 265 15 . . . cord-013178-li1x1m25 266 1 It -PRON- PRP cord-013178-li1x1m25 266 2 is be VBZ cord-013178-li1x1m25 266 3 very very RB cord-013178-li1x1m25 266 4 similar similar JJ cord-013178-li1x1m25 266 5 to to IN cord-013178-li1x1m25 266 6 the the DT cord-013178-li1x1m25 266 7 percentage percentage NN cord-013178-li1x1m25 266 8 of of IN cord-013178-li1x1m25 266 9 anti anti JJ cord-013178-li1x1m25 266 10 - - JJ cord-013178-li1x1m25 266 11 ORF3 ORF3 NNP cord-013178-li1x1m25 266 12 mAb mAb . cord-013178-li1x1m25 266 13 ( ( -LRB- cord-013178-li1x1m25 266 14 7D3 7d3 CD cord-013178-li1x1m25 266 15 ) ) -RRB- cord-013178-li1x1m25 266 16 staining staining NN cord-013178-li1x1m25 266 17 . . . cord-013178-li1x1m25 267 1 However however RB cord-013178-li1x1m25 267 2 , , , cord-013178-li1x1m25 267 3 these these DT cord-013178-li1x1m25 267 4 similar similar JJ cord-013178-li1x1m25 267 5 percentages percentage NNS cord-013178-li1x1m25 267 6 are be VBP cord-013178-li1x1m25 267 7 not not RB cord-013178-li1x1m25 267 8 sufficient sufficient JJ cord-013178-li1x1m25 267 9 proof proof NN cord-013178-li1x1m25 267 10 to to TO cord-013178-li1x1m25 267 11 show show VB cord-013178-li1x1m25 267 12 that that IN cord-013178-li1x1m25 267 13 the the DT cord-013178-li1x1m25 267 14 ORF3 ORF3 NNP cord-013178-li1x1m25 267 15 protein protein NN cord-013178-li1x1m25 267 16 of of IN cord-013178-li1x1m25 267 17 PCV2 PCV2 NNP cord-013178-li1x1m25 267 18 causes cause VBZ cord-013178-li1x1m25 267 19 DNA dna NN cord-013178-li1x1m25 267 20 damage damage NN cord-013178-li1x1m25 267 21 . . . cord-013178-li1x1m25 268 1 A a DT cord-013178-li1x1m25 268 2 more more RBR cord-013178-li1x1m25 268 3 careful careful JJ cord-013178-li1x1m25 268 4 analysis analysis NN cord-013178-li1x1m25 268 5 would would MD cord-013178-li1x1m25 268 6 be be VB cord-013178-li1x1m25 268 7 needed need VBN cord-013178-li1x1m25 268 8 to to TO cord-013178-li1x1m25 268 9 reveal reveal VB cord-013178-li1x1m25 268 10 whether whether IN cord-013178-li1x1m25 268 11 PCV2 pcv2 JJ cord-013178-li1x1m25 268 12 infection infection NN cord-013178-li1x1m25 268 13 causes cause VBZ cord-013178-li1x1m25 268 14 DNA dna NN cord-013178-li1x1m25 268 15 damage damage NN cord-013178-li1x1m25 268 16 . . . cord-013178-li1x1m25 269 1 Unfortunately unfortunately RB cord-013178-li1x1m25 269 2 , , , cord-013178-li1x1m25 269 3 it -PRON- PRP cord-013178-li1x1m25 269 4 was be VBD cord-013178-li1x1m25 269 5 not not RB cord-013178-li1x1m25 269 6 easy easy JJ cord-013178-li1x1m25 269 7 to to TO cord-013178-li1x1m25 269 8 label label VB cord-013178-li1x1m25 269 9 both both CC cord-013178-li1x1m25 269 10 the the DT cord-013178-li1x1m25 269 11 ORF3 ORF3 NNP cord-013178-li1x1m25 269 12 protein protein NN cord-013178-li1x1m25 269 13 and and CC cord-013178-li1x1m25 269 14 the the DT cord-013178-li1x1m25 269 15 signals signal NNS cord-013178-li1x1m25 269 16 of of IN cord-013178-li1x1m25 269 17 DNA dna NN cord-013178-li1x1m25 269 18 breakage breakage NN cord-013178-li1x1m25 269 19 simultaneously simultaneously RB cord-013178-li1x1m25 269 20 by by IN cord-013178-li1x1m25 269 21 IFA IFA NNP cord-013178-li1x1m25 269 22 and and CC cord-013178-li1x1m25 269 23 TUNEL TUNEL NNP cord-013178-li1x1m25 269 24 assay assay NN cord-013178-li1x1m25 269 25 ( ( -LRB- cord-013178-li1x1m25 269 26 data datum NNS cord-013178-li1x1m25 269 27 not not RB cord-013178-li1x1m25 269 28 shown show VBN cord-013178-li1x1m25 269 29 ) ) -RRB- cord-013178-li1x1m25 269 30 . . . cord-013178-li1x1m25 270 1 Further further JJ cord-013178-li1x1m25 270 2 research research NN cord-013178-li1x1m25 270 3 would would MD cord-013178-li1x1m25 270 4 be be VB cord-013178-li1x1m25 270 5 needed need VBN cord-013178-li1x1m25 270 6 to to TO cord-013178-li1x1m25 270 7 determine determine VB cord-013178-li1x1m25 270 8 whether whether IN cord-013178-li1x1m25 270 9 the the DT cord-013178-li1x1m25 270 10 ORF3 ORF3 NNP cord-013178-li1x1m25 270 11 protein protein NN cord-013178-li1x1m25 270 12 colocalizes colocalize VBZ cord-013178-li1x1m25 270 13 with with IN cord-013178-li1x1m25 270 14 the the DT cord-013178-li1x1m25 270 15 signals signal NNS cord-013178-li1x1m25 270 16 of of IN cord-013178-li1x1m25 270 17 TUNEL TUNEL NNP cord-013178-li1x1m25 270 18 . . . cord-013178-li1x1m25 271 1 6B8 6b8 LS cord-013178-li1x1m25 272 1 ( ( -LRB- cord-013178-li1x1m25 272 2 A a NN cord-013178-li1x1m25 272 3 ) ) -RRB- cord-013178-li1x1m25 272 4 ; ; : cord-013178-li1x1m25 273 1 mAb mab NN cord-013178-li1x1m25 273 2 1H3 1h3 CD cord-013178-li1x1m25 273 3 ( ( -LRB- cord-013178-li1x1m25 273 4 I i NN cord-013178-li1x1m25 273 5 ) ) -RRB- cord-013178-li1x1m25 273 6 ; ; : cord-013178-li1x1m25 273 7 and and CC cord-013178-li1x1m25 273 8 mAb mAb NNS cord-013178-li1x1m25 273 9 36A9 36a9 CD cord-013178-li1x1m25 273 10 ( ( -LRB- cord-013178-li1x1m25 273 11 M M NNP cord-013178-li1x1m25 273 12 ) ) -RRB- cord-013178-li1x1m25 273 13 ) ) -RRB- cord-013178-li1x1m25 273 14 , , , cord-013178-li1x1m25 273 15 and and CC cord-013178-li1x1m25 273 16 the the DT cord-013178-li1x1m25 273 17 ORF3 ORF3 NNP cord-013178-li1x1m25 273 18 peptide peptide NN cord-013178-li1x1m25 273 19 of of IN cord-013178-li1x1m25 273 20 PCV2 pcv2 JJ cord-013178-li1x1m25 273 21 ( ( -LRB- cord-013178-li1x1m25 273 22 mAb7D3 mAb7D3 NNP cord-013178-li1x1m25 273 23 ( ( -LRB- cord-013178-li1x1m25 273 24 E e UH cord-013178-li1x1m25 273 25 , , , cord-013178-li1x1m25 273 26 U U NNP cord-013178-li1x1m25 273 27 ) ) -RRB- cord-013178-li1x1m25 273 28 ) ) -RRB- cord-013178-li1x1m25 273 29 . . . cord-013178-li1x1m25 274 1 Staining stain VBG cord-013178-li1x1m25 274 2 with with IN cord-013178-li1x1m25 274 3 SPF SPF NNP cord-013178-li1x1m25 274 4 mouse mouse NN cord-013178-li1x1m25 274 5 serum serum NN cord-013178-li1x1m25 274 6 was be VBD cord-013178-li1x1m25 274 7 used use VBN cord-013178-li1x1m25 274 8 as as IN cord-013178-li1x1m25 274 9 the the DT cord-013178-li1x1m25 274 10 negative negative JJ cord-013178-li1x1m25 274 11 control control NN cord-013178-li1x1m25 274 12 ( ( -LRB- cord-013178-li1x1m25 274 13 Q Q NNP cord-013178-li1x1m25 274 14 ) ) -RRB- cord-013178-li1x1m25 274 15 . . . cord-013178-li1x1m25 275 1 ( ( -LRB- cord-013178-li1x1m25 275 2 B B NNP cord-013178-li1x1m25 275 3 , , , cord-013178-li1x1m25 275 4 F F NNP cord-013178-li1x1m25 275 5 , , , cord-013178-li1x1m25 275 6 J J NNP cord-013178-li1x1m25 275 7 , , , cord-013178-li1x1m25 275 8 N N NNP cord-013178-li1x1m25 275 9 , , , cord-013178-li1x1m25 275 10 V v NN cord-013178-li1x1m25 275 11 ) ) -RRB- cord-013178-li1x1m25 275 12 Staining stain VBG cord-013178-li1x1m25 275 13 with with IN cord-013178-li1x1m25 275 14 rabbit rabbit NN cord-013178-li1x1m25 275 15 antiserum antiserum NN cord-013178-li1x1m25 275 16 ( ( -LRB- cord-013178-li1x1m25 275 17 green green NNP cord-013178-li1x1m25 275 18 ) ) -RRB- cord-013178-li1x1m25 275 19 was be VBD cord-013178-li1x1m25 275 20 used use VBN cord-013178-li1x1m25 275 21 to to TO cord-013178-li1x1m25 275 22 identify identify VB cord-013178-li1x1m25 275 23 the the DT cord-013178-li1x1m25 275 24 VLP VLP NNP cord-013178-li1x1m25 275 25 of of IN cord-013178-li1x1m25 275 26 PCV2 PCV2 NNP cord-013178-li1x1m25 275 27 ( ( -LRB- cord-013178-li1x1m25 275 28 B B NNP cord-013178-li1x1m25 275 29 , , , cord-013178-li1x1m25 275 30 F F NNP cord-013178-li1x1m25 275 31 ) ) -RRB- cord-013178-li1x1m25 275 32 , , , cord-013178-li1x1m25 275 33 the the DT cord-013178-li1x1m25 275 34 p53 p53 NN cord-013178-li1x1m25 275 35 ( ( -LRB- cord-013178-li1x1m25 275 36 J J NNP cord-013178-li1x1m25 275 37 , , , cord-013178-li1x1m25 275 38 V V NNP cord-013178-li1x1m25 275 39 ) ) -RRB- cord-013178-li1x1m25 275 40 , , , cord-013178-li1x1m25 275 41 and and CC cord-013178-li1x1m25 275 42 the the DT cord-013178-li1x1m25 275 43 ORF3 ORF3 NNP cord-013178-li1x1m25 275 44 peptide peptide NN cord-013178-li1x1m25 275 45 of of IN cord-013178-li1x1m25 275 46 PCV2 PCV2 NNP cord-013178-li1x1m25 275 47 ( ( -LRB- cord-013178-li1x1m25 275 48 N N NNP cord-013178-li1x1m25 275 49 ) ) -RRB- cord-013178-li1x1m25 275 50 . . . cord-013178-li1x1m25 276 1 ( ( -LRB- cord-013178-li1x1m25 276 2 C C NNP cord-013178-li1x1m25 276 3 , , , cord-013178-li1x1m25 276 4 G G NNP cord-013178-li1x1m25 276 5 , , , cord-013178-li1x1m25 276 6 K K NNP cord-013178-li1x1m25 276 7 , , , cord-013178-li1x1m25 276 8 O o UH cord-013178-li1x1m25 276 9 , , , cord-013178-li1x1m25 276 10 S S NNP cord-013178-li1x1m25 276 11 , , , cord-013178-li1x1m25 276 12 W W NNP cord-013178-li1x1m25 276 13 , , , cord-013178-li1x1m25 276 14 Y Y NNP cord-013178-li1x1m25 276 15 ) ) -RRB- cord-013178-li1x1m25 276 16 Nuclei nucleus NNS cord-013178-li1x1m25 276 17 were be VBD cord-013178-li1x1m25 276 18 stained stain VBN cord-013178-li1x1m25 276 19 with with IN cord-013178-li1x1m25 276 20 DAPI dapi FW cord-013178-li1x1m25 276 21 ( ( -LRB- cord-013178-li1x1m25 276 22 blue blue JJ cord-013178-li1x1m25 276 23 ) ) -RRB- cord-013178-li1x1m25 276 24 . . . cord-013178-li1x1m25 277 1 The the DT cord-013178-li1x1m25 277 2 arrow arrow NN cord-013178-li1x1m25 277 3 points point VBZ cord-013178-li1x1m25 277 4 to to IN cord-013178-li1x1m25 277 5 the the DT cord-013178-li1x1m25 277 6 positive positive JJ cord-013178-li1x1m25 277 7 staining staining NN cord-013178-li1x1m25 277 8 cell cell NN cord-013178-li1x1m25 277 9 ( ( -LRB- cord-013178-li1x1m25 277 10 Z Z NNP cord-013178-li1x1m25 277 11 ) ) -RRB- cord-013178-li1x1m25 277 12 and and CC cord-013178-li1x1m25 277 13 its -PRON- PRP$ cord-013178-li1x1m25 277 14 irregular irregular JJ cord-013178-li1x1m25 277 15 shaped shaped JJ cord-013178-li1x1m25 277 16 nucleus nucleus NN cord-013178-li1x1m25 277 17 ( ( -LRB- cord-013178-li1x1m25 277 18 Y Y NNP cord-013178-li1x1m25 277 19 ) ) -RRB- cord-013178-li1x1m25 277 20 , , , cord-013178-li1x1m25 277 21 and and CC cord-013178-li1x1m25 277 22 the the DT cord-013178-li1x1m25 277 23 enlarged enlarged JJ cord-013178-li1x1m25 277 24 image image NN cord-013178-li1x1m25 277 25 in in IN cord-013178-li1x1m25 277 26 the the DT cord-013178-li1x1m25 277 27 sixth sixth JJ cord-013178-li1x1m25 277 28 row row NN cord-013178-li1x1m25 277 29 ( ( -LRB- cord-013178-li1x1m25 277 30 X X NNP cord-013178-li1x1m25 277 31 , , , cord-013178-li1x1m25 277 32 W W NNP cord-013178-li1x1m25 277 33 ) ) -RRB- cord-013178-li1x1m25 277 34 . . . cord-013178-li1x1m25 278 1 ( ( -LRB- cord-013178-li1x1m25 278 2 D D NNP cord-013178-li1x1m25 278 3 , , , cord-013178-li1x1m25 278 4 H H NNP cord-013178-li1x1m25 278 5 , , , cord-013178-li1x1m25 278 6 L L NNP cord-013178-li1x1m25 278 7 , , , cord-013178-li1x1m25 278 8 P P NNP cord-013178-li1x1m25 278 9 , , , cord-013178-li1x1m25 278 10 T T NNP cord-013178-li1x1m25 278 11 , , , cord-013178-li1x1m25 278 12 X X NNP cord-013178-li1x1m25 278 13 , , , cord-013178-li1x1m25 278 14 Z z NN cord-013178-li1x1m25 278 15 ) ) -RRB- cord-013178-li1x1m25 279 1 The the DT cord-013178-li1x1m25 279 2 merged merged JJ cord-013178-li1x1m25 279 3 images image NNS cord-013178-li1x1m25 279 4 are be VBP cord-013178-li1x1m25 279 5 also also RB cord-013178-li1x1m25 279 6 shown show VBN cord-013178-li1x1m25 279 7 . . . cord-013178-li1x1m25 280 1 The the DT cord-013178-li1x1m25 280 2 scale scale NN cord-013178-li1x1m25 280 3 bar bar NN cord-013178-li1x1m25 280 4 is be VBZ cord-013178-li1x1m25 280 5 10 10 CD cord-013178-li1x1m25 280 6 μm μm NN cord-013178-li1x1m25 280 7 . . . cord-013178-li1x1m25 280 8 _SP cord-013178-li1x1m25 281 1 The the DT cord-013178-li1x1m25 281 2 viral viral JJ cord-013178-li1x1m25 281 3 proteins protein NNS cord-013178-li1x1m25 281 4 were be VBD cord-013178-li1x1m25 281 5 detected detect VBN cord-013178-li1x1m25 281 6 in in IN cord-013178-li1x1m25 281 7 the the DT cord-013178-li1x1m25 281 8 whole whole JJ cord-013178-li1x1m25 281 9 - - HYPH cord-013178-li1x1m25 281 10 blood blood NN cord-013178-li1x1m25 281 11 samples sample NNS cord-013178-li1x1m25 281 12 using use VBG cord-013178-li1x1m25 281 13 antigen antigen NN cord-013178-li1x1m25 281 14 - - HYPH cord-013178-li1x1m25 281 15 ELISA ELISA NNP cord-013178-li1x1m25 281 16 techniques technique NNS cord-013178-li1x1m25 281 17 . . . cord-013178-li1x1m25 282 1 The the DT cord-013178-li1x1m25 282 2 cut cut VB cord-013178-li1x1m25 282 3 - - HYPH cord-013178-li1x1m25 282 4 off off RP cord-013178-li1x1m25 282 5 value value NN cord-013178-li1x1m25 282 6 in in IN cord-013178-li1x1m25 282 7 the the DT cord-013178-li1x1m25 282 8 commercial commercial JJ cord-013178-li1x1m25 282 9 PCV2 PCV2 NNP cord-013178-li1x1m25 282 10 capsid capsid NN cord-013178-li1x1m25 282 11 ELISA ELISA NNP cord-013178-li1x1m25 282 12 and and CC cord-013178-li1x1m25 282 13 the the DT cord-013178-li1x1m25 282 14 ORF3 ORF3 NNP cord-013178-li1x1m25 282 15 protein protein NN cord-013178-li1x1m25 282 16 ELISA ELISA NNP cord-013178-li1x1m25 282 17 were be VBD cord-013178-li1x1m25 282 18 0.15 0.15 CD cord-013178-li1x1m25 282 19 and and CC cord-013178-li1x1m25 282 20 0.22 0.22 CD cord-013178-li1x1m25 282 21 , , , cord-013178-li1x1m25 282 22 respectively respectively RB cord-013178-li1x1m25 282 23 . . . cord-013178-li1x1m25 283 1 The the DT cord-013178-li1x1m25 283 2 capsid capsid JJ cord-013178-li1x1m25 283 3 protein protein NN cord-013178-li1x1m25 283 4 of of IN cord-013178-li1x1m25 283 5 PCV2 PCV2 NNP cord-013178-li1x1m25 283 6 was be VBD cord-013178-li1x1m25 283 7 mainly mainly RB cord-013178-li1x1m25 283 8 detected detect VBN cord-013178-li1x1m25 283 9 in in IN cord-013178-li1x1m25 283 10 pigs pig NNS cord-013178-li1x1m25 283 11 at at IN cord-013178-li1x1m25 283 12 1 1 CD cord-013178-li1x1m25 283 13 , , , cord-013178-li1x1m25 283 14 3 3 CD cord-013178-li1x1m25 283 15 , , , cord-013178-li1x1m25 283 16 6 6 CD cord-013178-li1x1m25 283 17 , , , cord-013178-li1x1m25 283 18 and and CC cord-013178-li1x1m25 283 19 ≥34 ≥34 NNP cord-013178-li1x1m25 283 20 weeks week NNS cord-013178-li1x1m25 283 21 of of IN cord-013178-li1x1m25 283 22 age age NN cord-013178-li1x1m25 283 23 in in IN cord-013178-li1x1m25 283 24 this this DT cord-013178-li1x1m25 283 25 study study NN cord-013178-li1x1m25 283 26 farm farm NN cord-013178-li1x1m25 283 27 ( ( -LRB- cord-013178-li1x1m25 283 28 Figure figure NN cord-013178-li1x1m25 283 29 5A 5a NN cord-013178-li1x1m25 283 30 ) ) -RRB- cord-013178-li1x1m25 283 31 . . . cord-013178-li1x1m25 284 1 Noticeably noticeably RB cord-013178-li1x1m25 284 2 , , , cord-013178-li1x1m25 284 3 these these DT cord-013178-li1x1m25 284 4 groups group NNS cord-013178-li1x1m25 284 5 ( ( -LRB- cord-013178-li1x1m25 284 6 at at IN cord-013178-li1x1m25 284 7 1 1 CD cord-013178-li1x1m25 284 8 , , , cord-013178-li1x1m25 284 9 3 3 CD cord-013178-li1x1m25 284 10 , , , cord-013178-li1x1m25 284 11 6 6 CD cord-013178-li1x1m25 284 12 , , , cord-013178-li1x1m25 284 13 and and CC cord-013178-li1x1m25 284 14 ≥34 ≥34 NNP cord-013178-li1x1m25 284 15 weeks week NNS cord-013178-li1x1m25 284 16 of of IN cord-013178-li1x1m25 284 17 age age NN cord-013178-li1x1m25 284 18 ) ) -RRB- cord-013178-li1x1m25 284 19 showed show VBD cord-013178-li1x1m25 284 20 more more RBR cord-013178-li1x1m25 284 21 elevated elevated JJ cord-013178-li1x1m25 284 22 standard standard JJ cord-013178-li1x1m25 284 23 error error NN cord-013178-li1x1m25 284 24 of of IN cord-013178-li1x1m25 284 25 OD OD NNP cord-013178-li1x1m25 284 26 405 405 CD cord-013178-li1x1m25 284 27 value value NN cord-013178-li1x1m25 284 28 than than IN cord-013178-li1x1m25 284 29 other other JJ cord-013178-li1x1m25 284 30 groups group NNS cord-013178-li1x1m25 284 31 in in IN cord-013178-li1x1m25 284 32 this this DT cord-013178-li1x1m25 284 33 statistical statistical JJ cord-013178-li1x1m25 284 34 analysis analysis NN cord-013178-li1x1m25 284 35 . . . cord-013178-li1x1m25 285 1 This this DT cord-013178-li1x1m25 285 2 result result NN cord-013178-li1x1m25 285 3 implies imply VBZ cord-013178-li1x1m25 285 4 that that IN cord-013178-li1x1m25 285 5 pigs pig NNS cord-013178-li1x1m25 285 6 at at IN cord-013178-li1x1m25 285 7 that that DT cord-013178-li1x1m25 285 8 age age NN cord-013178-li1x1m25 285 9 would would MD cord-013178-li1x1m25 285 10 be be VB cord-013178-li1x1m25 285 11 susceptible susceptible JJ cord-013178-li1x1m25 285 12 to to IN cord-013178-li1x1m25 285 13 PCV2 pcv2 JJ cord-013178-li1x1m25 285 14 infection infection NN cord-013178-li1x1m25 285 15 . . . cord-013178-li1x1m25 286 1 Interestingly interestingly RB cord-013178-li1x1m25 286 2 , , , cord-013178-li1x1m25 286 3 non non JJ cord-013178-li1x1m25 286 4 - - JJ cord-013178-li1x1m25 286 5 vaccinated vaccinated JJ cord-013178-li1x1m25 286 6 piglets piglet NNS cord-013178-li1x1m25 286 7 showed show VBD cord-013178-li1x1m25 286 8 the the DT cord-013178-li1x1m25 286 9 highest high JJS cord-013178-li1x1m25 286 10 OD OD NNP cord-013178-li1x1m25 286 11 450 450 CD cord-013178-li1x1m25 286 12 value value NN cord-013178-li1x1m25 286 13 of of IN cord-013178-li1x1m25 286 14 capsid capsid NN cord-013178-li1x1m25 286 15 protein protein NN cord-013178-li1x1m25 286 16 at at IN cord-013178-li1x1m25 286 17 one one CD cord-013178-li1x1m25 286 18 week week NN cord-013178-li1x1m25 286 19 of of IN cord-013178-li1x1m25 286 20 age age NN cord-013178-li1x1m25 286 21 . . . cord-013178-li1x1m25 287 1 After after IN cord-013178-li1x1m25 287 2 piglets piglet NNS cord-013178-li1x1m25 287 3 were be VBD cord-013178-li1x1m25 287 4 inoculated inoculate VBN cord-013178-li1x1m25 287 5 with with IN cord-013178-li1x1m25 287 6 the the DT cord-013178-li1x1m25 287 7 PCV2 PCV2 NNP cord-013178-li1x1m25 287 8 vaccine vaccine NN cord-013178-li1x1m25 287 9 at at IN cord-013178-li1x1m25 287 10 two two CD cord-013178-li1x1m25 287 11 weeks week NNS cord-013178-li1x1m25 287 12 of of IN cord-013178-li1x1m25 287 13 age age NN cord-013178-li1x1m25 287 14 , , , cord-013178-li1x1m25 287 15 the the DT cord-013178-li1x1m25 287 16 OD OD NNP cord-013178-li1x1m25 287 17 450 450 CD cord-013178-li1x1m25 287 18 value value NN cord-013178-li1x1m25 287 19 of of IN cord-013178-li1x1m25 287 20 capsid capsid NN cord-013178-li1x1m25 287 21 protein protein NN cord-013178-li1x1m25 287 22 gradually gradually RB cord-013178-li1x1m25 287 23 decreased decrease VBD cord-013178-li1x1m25 287 24 until until IN cord-013178-li1x1m25 287 25 24 24 CD cord-013178-li1x1m25 287 26 weeks week NNS cord-013178-li1x1m25 287 27 of of IN cord-013178-li1x1m25 287 28 age age NN cord-013178-li1x1m25 287 29 . . . cord-013178-li1x1m25 288 1 However however RB cord-013178-li1x1m25 288 2 , , , cord-013178-li1x1m25 288 3 the the DT cord-013178-li1x1m25 288 4 OD OD NNP cord-013178-li1x1m25 288 5 450 450 CD cord-013178-li1x1m25 288 6 value value NN cord-013178-li1x1m25 288 7 of of IN cord-013178-li1x1m25 288 8 capsid capsid NN cord-013178-li1x1m25 288 9 protein protein NN cord-013178-li1x1m25 288 10 was be VBD cord-013178-li1x1m25 288 11 raised raise VBN cord-013178-li1x1m25 288 12 at at IN cord-013178-li1x1m25 288 13 ≥34 ≥34 NNP cord-013178-li1x1m25 288 14 weeks week NNS cord-013178-li1x1m25 288 15 of of IN cord-013178-li1x1m25 288 16 age age NN cord-013178-li1x1m25 288 17 ( ( -LRB- cord-013178-li1x1m25 288 18 two two CD cord-013178-li1x1m25 288 19 gilts gilt NNS cord-013178-li1x1m25 288 20 and and CC cord-013178-li1x1m25 288 21 two two CD cord-013178-li1x1m25 288 22 sows sow NNS cord-013178-li1x1m25 288 23 ) ) -RRB- cord-013178-li1x1m25 288 24 . . . cord-013178-li1x1m25 289 1 There there EX cord-013178-li1x1m25 289 2 is be VBZ cord-013178-li1x1m25 289 3 evidence evidence NN cord-013178-li1x1m25 289 4 to to TO cord-013178-li1x1m25 289 5 suggest suggest VB cord-013178-li1x1m25 289 6 that that IN cord-013178-li1x1m25 289 7 the the DT cord-013178-li1x1m25 289 8 vaccine vaccine NN cord-013178-li1x1m25 289 9 could could MD cord-013178-li1x1m25 289 10 not not RB cord-013178-li1x1m25 289 11 protect protect VB cord-013178-li1x1m25 289 12 pigs pig NNS cord-013178-li1x1m25 289 13 against against IN cord-013178-li1x1m25 289 14 PCV2 pcv2 JJ cord-013178-li1x1m25 289 15 infection infection NN cord-013178-li1x1m25 289 16 at at IN cord-013178-li1x1m25 289 17 32 32 CD cord-013178-li1x1m25 289 18 weeks week NNS cord-013178-li1x1m25 289 19 postimmunization postimmunization NN cord-013178-li1x1m25 289 20 . . . cord-013178-li1x1m25 290 1 The the DT cord-013178-li1x1m25 290 2 ORF3 ORF3 NNP cord-013178-li1x1m25 290 3 protein protein NN cord-013178-li1x1m25 290 4 of of IN cord-013178-li1x1m25 290 5 PCV2 PCV2 NNPS cord-013178-li1x1m25 290 6 showed show VBD cord-013178-li1x1m25 290 7 the the DT cord-013178-li1x1m25 290 8 same same JJ cord-013178-li1x1m25 290 9 result result NN cord-013178-li1x1m25 290 10 except except IN cord-013178-li1x1m25 290 11 for for IN cord-013178-li1x1m25 290 12 OD OD NNP cord-013178-li1x1m25 290 13 values value NNS cord-013178-li1x1m25 290 14 of of IN cord-013178-li1x1m25 290 15 the the DT cord-013178-li1x1m25 290 16 ORF3 ORF3 NNP cord-013178-li1x1m25 290 17 protein protein NN cord-013178-li1x1m25 290 18 were be VBD cord-013178-li1x1m25 290 19 lower low JJR cord-013178-li1x1m25 290 20 than than IN cord-013178-li1x1m25 290 21 the the DT cord-013178-li1x1m25 290 22 capsid capsid NN cord-013178-li1x1m25 290 23 protein protein NN cord-013178-li1x1m25 290 24 . . . cord-013178-li1x1m25 291 1 The the DT cord-013178-li1x1m25 291 2 correlation correlation NN cord-013178-li1x1m25 291 3 coefficient coefficient NN cord-013178-li1x1m25 291 4 ( ( -LRB- cord-013178-li1x1m25 291 5 r r NN cord-013178-li1x1m25 291 6 ) ) -RRB- cord-013178-li1x1m25 291 7 was be VBD cord-013178-li1x1m25 291 8 0.7583 0.7583 CD cord-013178-li1x1m25 291 9 . . . cord-013178-li1x1m25 292 1 The the DT cord-013178-li1x1m25 292 2 mean mean JJ cord-013178-li1x1m25 292 3 OD OD NNP cord-013178-li1x1m25 292 4 405 405 CD cord-013178-li1x1m25 292 5 values value NNS cord-013178-li1x1m25 292 6 of of IN cord-013178-li1x1m25 292 7 the the DT cord-013178-li1x1m25 292 8 ORF3 ORF3 NNP cord-013178-li1x1m25 292 9 protein protein NN cord-013178-li1x1m25 292 10 were be VBD cord-013178-li1x1m25 292 11 significantly significantly RB cord-013178-li1x1m25 292 12 higher high JJR cord-013178-li1x1m25 292 13 for for IN cord-013178-li1x1m25 292 14 suckers sucker NNS cord-013178-li1x1m25 292 15 at at IN cord-013178-li1x1m25 292 16 one one CD cord-013178-li1x1m25 292 17 week week NN cord-013178-li1x1m25 292 18 of of IN cord-013178-li1x1m25 292 19 age age NN cord-013178-li1x1m25 292 20 than than IN cord-013178-li1x1m25 292 21 postweaning postweane VBG cord-013178-li1x1m25 292 22 ones one NNS cord-013178-li1x1m25 292 23 at at IN cord-013178-li1x1m25 292 24 9 9 CD cord-013178-li1x1m25 292 25 - - SYM cord-013178-li1x1m25 292 26 24 24 CD cord-013178-li1x1m25 292 27 weeks week NNS cord-013178-li1x1m25 292 28 of of IN cord-013178-li1x1m25 292 29 age age NN cord-013178-li1x1m25 292 30 ( ( -LRB- cord-013178-li1x1m25 292 31 p p NN cord-013178-li1x1m25 292 32 < < XX cord-013178-li1x1m25 292 33 0.05 0.05 CD cord-013178-li1x1m25 292 34 ; ; : cord-013178-li1x1m25 292 35 Figure figure NN cord-013178-li1x1m25 292 36 5B 5b NN cord-013178-li1x1m25 292 37 ) ) -RRB- cord-013178-li1x1m25 292 38 . . . cord-013178-li1x1m25 293 1 There there EX cord-013178-li1x1m25 293 2 is be VBZ cord-013178-li1x1m25 293 3 an an DT cord-013178-li1x1m25 293 4 apparent apparent JJ cord-013178-li1x1m25 293 5 probability probability NN cord-013178-li1x1m25 293 6 that that IN cord-013178-li1x1m25 293 7 the the DT cord-013178-li1x1m25 293 8 amount amount NN cord-013178-li1x1m25 293 9 of of IN cord-013178-li1x1m25 293 10 ORF3 ORF3 NNP cord-013178-li1x1m25 293 11 protein protein NN cord-013178-li1x1m25 293 12 was be VBD cord-013178-li1x1m25 293 13 fewer few JJR cord-013178-li1x1m25 293 14 than than IN cord-013178-li1x1m25 293 15 the the DT cord-013178-li1x1m25 293 16 amount amount NN cord-013178-li1x1m25 293 17 of of IN cord-013178-li1x1m25 293 18 capsid capsid NN cord-013178-li1x1m25 293 19 protein protein NN cord-013178-li1x1m25 293 20 in in IN cord-013178-li1x1m25 293 21 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 293 22 blood blood NN cord-013178-li1x1m25 293 23 from from IN cord-013178-li1x1m25 293 24 suckers sucker NNS cord-013178-li1x1m25 293 25 at at IN cord-013178-li1x1m25 293 26 one one CD cord-013178-li1x1m25 293 27 week week NN cord-013178-li1x1m25 293 28 of of IN cord-013178-li1x1m25 293 29 age age NN cord-013178-li1x1m25 293 30 . . . cord-013178-li1x1m25 294 1 protect protect VB cord-013178-li1x1m25 294 2 pigs pig NNS cord-013178-li1x1m25 294 3 against against IN cord-013178-li1x1m25 294 4 PCV2 pcv2 JJ cord-013178-li1x1m25 294 5 infection infection NN cord-013178-li1x1m25 294 6 at at IN cord-013178-li1x1m25 294 7 32 32 CD cord-013178-li1x1m25 294 8 weeks week NNS cord-013178-li1x1m25 294 9 postimmunization postimmunization NN cord-013178-li1x1m25 294 10 . . . cord-013178-li1x1m25 295 1 The the DT cord-013178-li1x1m25 295 2 ORF3 ORF3 NNP cord-013178-li1x1m25 295 3 protein protein NN cord-013178-li1x1m25 295 4 of of IN cord-013178-li1x1m25 295 5 PCV2 PCV2 NNPS cord-013178-li1x1m25 295 6 showed show VBD cord-013178-li1x1m25 295 7 the the DT cord-013178-li1x1m25 295 8 same same JJ cord-013178-li1x1m25 295 9 result result NN cord-013178-li1x1m25 295 10 except except IN cord-013178-li1x1m25 295 11 for for IN cord-013178-li1x1m25 295 12 OD OD NNP cord-013178-li1x1m25 295 13 values value NNS cord-013178-li1x1m25 295 14 of of IN cord-013178-li1x1m25 295 15 the the DT cord-013178-li1x1m25 295 16 ORF3 ORF3 NNP cord-013178-li1x1m25 295 17 protein protein NN cord-013178-li1x1m25 295 18 were be VBD cord-013178-li1x1m25 295 19 lower low JJR cord-013178-li1x1m25 295 20 than than IN cord-013178-li1x1m25 295 21 the the DT cord-013178-li1x1m25 295 22 capsid capsid NN cord-013178-li1x1m25 295 23 protein protein NN cord-013178-li1x1m25 295 24 . . . cord-013178-li1x1m25 296 1 The the DT cord-013178-li1x1m25 296 2 correlation correlation NN cord-013178-li1x1m25 296 3 coefficient coefficient NN cord-013178-li1x1m25 296 4 ( ( -LRB- cord-013178-li1x1m25 296 5 r r NN cord-013178-li1x1m25 296 6 ) ) -RRB- cord-013178-li1x1m25 296 7 was be VBD cord-013178-li1x1m25 296 8 0.7583 0.7583 CD cord-013178-li1x1m25 296 9 . . . cord-013178-li1x1m25 297 1 The the DT cord-013178-li1x1m25 297 2 mean mean NN cord-013178-li1x1m25 297 3 OD405 od405 JJ cord-013178-li1x1m25 297 4 values value NNS cord-013178-li1x1m25 297 5 of of IN cord-013178-li1x1m25 297 6 the the DT cord-013178-li1x1m25 297 7 ORF3 ORF3 NNP cord-013178-li1x1m25 297 8 protein protein NN cord-013178-li1x1m25 297 9 were be VBD cord-013178-li1x1m25 297 10 significantly significantly RB cord-013178-li1x1m25 297 11 higher high JJR cord-013178-li1x1m25 297 12 for for IN cord-013178-li1x1m25 297 13 suckers sucker NNS cord-013178-li1x1m25 297 14 at at IN cord-013178-li1x1m25 297 15 one one CD cord-013178-li1x1m25 297 16 week week NN cord-013178-li1x1m25 297 17 of of IN cord-013178-li1x1m25 297 18 age age NN cord-013178-li1x1m25 297 19 than than IN cord-013178-li1x1m25 297 20 postweaning postweane VBG cord-013178-li1x1m25 297 21 ones one NNS cord-013178-li1x1m25 297 22 at at IN cord-013178-li1x1m25 297 23 9 9 CD cord-013178-li1x1m25 297 24 - - SYM cord-013178-li1x1m25 297 25 24 24 CD cord-013178-li1x1m25 297 26 weeks week NNS cord-013178-li1x1m25 297 27 of of IN cord-013178-li1x1m25 297 28 age age NN cord-013178-li1x1m25 297 29 ( ( -LRB- cord-013178-li1x1m25 297 30 p p NN cord-013178-li1x1m25 297 31 < < XX cord-013178-li1x1m25 297 32 0.05 0.05 CD cord-013178-li1x1m25 297 33 ; ; : cord-013178-li1x1m25 297 34 Figure figure NN cord-013178-li1x1m25 297 35 5B 5b NN cord-013178-li1x1m25 297 36 ) ) -RRB- cord-013178-li1x1m25 297 37 . . . cord-013178-li1x1m25 298 1 There there EX cord-013178-li1x1m25 298 2 is be VBZ cord-013178-li1x1m25 298 3 an an DT cord-013178-li1x1m25 298 4 apparent apparent JJ cord-013178-li1x1m25 298 5 probability probability NN cord-013178-li1x1m25 298 6 that that IN cord-013178-li1x1m25 298 7 the the DT cord-013178-li1x1m25 298 8 amount amount NN cord-013178-li1x1m25 298 9 of of IN cord-013178-li1x1m25 298 10 ORF3 ORF3 NNP cord-013178-li1x1m25 298 11 protein protein NN cord-013178-li1x1m25 298 12 was be VBD cord-013178-li1x1m25 298 13 fewer few JJR cord-013178-li1x1m25 298 14 than than IN cord-013178-li1x1m25 298 15 the the DT cord-013178-li1x1m25 298 16 amount amount NN cord-013178-li1x1m25 298 17 of of IN cord-013178-li1x1m25 298 18 capsid capsid NN cord-013178-li1x1m25 298 19 protein protein NN cord-013178-li1x1m25 298 20 in in IN cord-013178-li1x1m25 298 21 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 298 22 blood blood NN cord-013178-li1x1m25 298 23 from from IN cord-013178-li1x1m25 298 24 suckers sucker NNS cord-013178-li1x1m25 298 25 at at IN cord-013178-li1x1m25 298 26 one one CD cord-013178-li1x1m25 298 27 week week NN cord-013178-li1x1m25 298 28 of of IN cord-013178-li1x1m25 298 29 age age NN cord-013178-li1x1m25 298 30 . . . cord-013178-li1x1m25 299 1 Figure figure NN cord-013178-li1x1m25 299 2 5 5 CD cord-013178-li1x1m25 299 3 . . . cord-013178-li1x1m25 300 1 Assessment assessment NN cord-013178-li1x1m25 300 2 of of IN cord-013178-li1x1m25 300 3 the the DT cord-013178-li1x1m25 300 4 capsid capsid NN cord-013178-li1x1m25 300 5 protein protein NN cord-013178-li1x1m25 300 6 and and CC cord-013178-li1x1m25 300 7 the the DT cord-013178-li1x1m25 300 8 ORF3 ORF3 NNP cord-013178-li1x1m25 300 9 protein protein NN cord-013178-li1x1m25 300 10 in in IN cord-013178-li1x1m25 300 11 the the DT cord-013178-li1x1m25 300 12 whole whole JJ cord-013178-li1x1m25 300 13 - - HYPH cord-013178-li1x1m25 300 14 blood blood NN cord-013178-li1x1m25 300 15 samples sample NNS cord-013178-li1x1m25 300 16 from from IN cord-013178-li1x1m25 300 17 pigs pig NNS cord-013178-li1x1m25 300 18 of of IN cord-013178-li1x1m25 300 19 different different JJ cord-013178-li1x1m25 300 20 age age NN cord-013178-li1x1m25 300 21 groups group NNS cord-013178-li1x1m25 300 22 . . . cord-013178-li1x1m25 301 1 This this DT cord-013178-li1x1m25 301 2 study study NN cord-013178-li1x1m25 301 3 used use VBD cord-013178-li1x1m25 301 4 the the DT cord-013178-li1x1m25 301 5 commercial commercial JJ cord-013178-li1x1m25 301 6 capsid capsid NN cord-013178-li1x1m25 301 7 kit kit NN cord-013178-li1x1m25 301 8 ( ( -LRB- cord-013178-li1x1m25 301 9 A a NN cord-013178-li1x1m25 301 10 ) ) -RRB- cord-013178-li1x1m25 301 11 and and CC cord-013178-li1x1m25 301 12 the the DT cord-013178-li1x1m25 301 13 ORF3 ORF3 NNP cord-013178-li1x1m25 301 14 protein protein NN cord-013178-li1x1m25 301 15 ELISA ELISA NNP cord-013178-li1x1m25 301 16 ( ( -LRB- cord-013178-li1x1m25 301 17 B b NN cord-013178-li1x1m25 301 18 ) ) -RRB- cord-013178-li1x1m25 301 19 to to TO cord-013178-li1x1m25 301 20 measure measure VB cord-013178-li1x1m25 301 21 each each DT cord-013178-li1x1m25 301 22 specimen specimen NN cord-013178-li1x1m25 301 23 , , , cord-013178-li1x1m25 301 24 and and CC cord-013178-li1x1m25 301 25 the the DT cord-013178-li1x1m25 301 26 absorbance absorbance NN cord-013178-li1x1m25 301 27 was be VBD cord-013178-li1x1m25 301 28 measured measure VBN cord-013178-li1x1m25 301 29 at at IN cord-013178-li1x1m25 301 30 450 450 CD cord-013178-li1x1m25 301 31 or or CC cord-013178-li1x1m25 301 32 405 405 CD cord-013178-li1x1m25 301 33 nm nm NN cord-013178-li1x1m25 301 34 , , , cord-013178-li1x1m25 301 35 respectively respectively RB cord-013178-li1x1m25 301 36 . . . cord-013178-li1x1m25 302 1 The the DT cord-013178-li1x1m25 302 2 peptide peptide NN cord-013178-li1x1m25 302 3 N1 n1 NN cord-013178-li1x1m25 302 4 and and CC cord-013178-li1x1m25 302 5 PSBT PSBT NNP cord-013178-li1x1m25 302 6 were be VBD cord-013178-li1x1m25 302 7 used use VBN cord-013178-li1x1m25 302 8 as as IN cord-013178-li1x1m25 302 9 a a DT cord-013178-li1x1m25 302 10 positive positive JJ cord-013178-li1x1m25 302 11 ( ( -LRB- cord-013178-li1x1m25 302 12 pos pos NNP cord-013178-li1x1m25 302 13 ) ) -RRB- cord-013178-li1x1m25 302 14 control control NN cord-013178-li1x1m25 302 15 and and CC cord-013178-li1x1m25 302 16 a a DT cord-013178-li1x1m25 302 17 negative negative JJ cord-013178-li1x1m25 302 18 ( ( -LRB- cord-013178-li1x1m25 302 19 neg neg NNP cord-013178-li1x1m25 302 20 ) ) -RRB- cord-013178-li1x1m25 302 21 control control NN cord-013178-li1x1m25 302 22 , , , cord-013178-li1x1m25 302 23 respectively respectively RB cord-013178-li1x1m25 302 24 , , , cord-013178-li1x1m25 302 25 in in IN cord-013178-li1x1m25 302 26 the the DT cord-013178-li1x1m25 302 27 ORF3 ORF3 NNP cord-013178-li1x1m25 302 28 protein protein NN cord-013178-li1x1m25 302 29 ELISA ELISA NNP cord-013178-li1x1m25 302 30 . . . cord-013178-li1x1m25 303 1 The the DT cord-013178-li1x1m25 303 2 data datum NNS cord-013178-li1x1m25 303 3 represent represent VBP cord-013178-li1x1m25 303 4 the the DT cord-013178-li1x1m25 303 5 mean mean JJ cord-013178-li1x1m25 303 6 ± ± NNP cord-013178-li1x1m25 303 7 SE SE NNP cord-013178-li1x1m25 303 8 . . . cord-013178-li1x1m25 304 1 Treatments treatment NNS cord-013178-li1x1m25 304 2 with with IN cord-013178-li1x1m25 304 3 different different JJ cord-013178-li1x1m25 304 4 letters letter NNS cord-013178-li1x1m25 304 5 have have VBP cord-013178-li1x1m25 304 6 statistically statistically RB cord-013178-li1x1m25 304 7 significant significant JJ cord-013178-li1x1m25 304 8 differences difference NNS cord-013178-li1x1m25 304 9 ( ( -LRB- cord-013178-li1x1m25 304 10 p p NN cord-013178-li1x1m25 304 11 < < XX cord-013178-li1x1m25 304 12 0.05 0.05 CD cord-013178-li1x1m25 304 13 ) ) -RRB- cord-013178-li1x1m25 304 14 . . . cord-013178-li1x1m25 305 1 Figure figure NN cord-013178-li1x1m25 305 2 5 5 CD cord-013178-li1x1m25 305 3 . . . cord-013178-li1x1m25 306 1 Assessment assessment NN cord-013178-li1x1m25 306 2 of of IN cord-013178-li1x1m25 306 3 the the DT cord-013178-li1x1m25 306 4 capsid capsid NN cord-013178-li1x1m25 306 5 protein protein NN cord-013178-li1x1m25 306 6 and and CC cord-013178-li1x1m25 306 7 the the DT cord-013178-li1x1m25 306 8 ORF3 ORF3 NNP cord-013178-li1x1m25 306 9 protein protein NN cord-013178-li1x1m25 306 10 in in IN cord-013178-li1x1m25 306 11 the the DT cord-013178-li1x1m25 306 12 whole whole JJ cord-013178-li1x1m25 306 13 - - HYPH cord-013178-li1x1m25 306 14 blood blood NN cord-013178-li1x1m25 306 15 samples sample NNS cord-013178-li1x1m25 306 16 from from IN cord-013178-li1x1m25 306 17 pigs pig NNS cord-013178-li1x1m25 306 18 of of IN cord-013178-li1x1m25 306 19 different different JJ cord-013178-li1x1m25 306 20 age age NN cord-013178-li1x1m25 306 21 groups group NNS cord-013178-li1x1m25 306 22 . . . cord-013178-li1x1m25 307 1 This this DT cord-013178-li1x1m25 307 2 study study NN cord-013178-li1x1m25 307 3 used use VBD cord-013178-li1x1m25 307 4 the the DT cord-013178-li1x1m25 307 5 commercial commercial JJ cord-013178-li1x1m25 307 6 capsid capsid NN cord-013178-li1x1m25 307 7 kit kit NN cord-013178-li1x1m25 307 8 ( ( -LRB- cord-013178-li1x1m25 307 9 A a NN cord-013178-li1x1m25 307 10 ) ) -RRB- cord-013178-li1x1m25 307 11 and and CC cord-013178-li1x1m25 307 12 the the DT cord-013178-li1x1m25 307 13 ORF3 ORF3 NNP cord-013178-li1x1m25 307 14 protein protein NN cord-013178-li1x1m25 307 15 ELISA ELISA NNP cord-013178-li1x1m25 307 16 ( ( -LRB- cord-013178-li1x1m25 307 17 B b NN cord-013178-li1x1m25 307 18 ) ) -RRB- cord-013178-li1x1m25 307 19 to to TO cord-013178-li1x1m25 307 20 measure measure VB cord-013178-li1x1m25 307 21 each each DT cord-013178-li1x1m25 307 22 specimen specimen NN cord-013178-li1x1m25 307 23 , , , cord-013178-li1x1m25 307 24 and and CC cord-013178-li1x1m25 307 25 the the DT cord-013178-li1x1m25 307 26 absorbance absorbance NN cord-013178-li1x1m25 307 27 was be VBD cord-013178-li1x1m25 307 28 measured measure VBN cord-013178-li1x1m25 307 29 at at IN cord-013178-li1x1m25 307 30 450 450 CD cord-013178-li1x1m25 307 31 or or CC cord-013178-li1x1m25 307 32 405 405 CD cord-013178-li1x1m25 307 33 nm nm NN cord-013178-li1x1m25 307 34 , , , cord-013178-li1x1m25 307 35 respectively respectively RB cord-013178-li1x1m25 307 36 . . . cord-013178-li1x1m25 308 1 The the DT cord-013178-li1x1m25 308 2 peptide peptide NN cord-013178-li1x1m25 308 3 N1 n1 NN cord-013178-li1x1m25 308 4 and and CC cord-013178-li1x1m25 308 5 PSBT PSBT NNP cord-013178-li1x1m25 308 6 were be VBD cord-013178-li1x1m25 308 7 used use VBN cord-013178-li1x1m25 308 8 as as IN cord-013178-li1x1m25 308 9 a a DT cord-013178-li1x1m25 308 10 positive positive JJ cord-013178-li1x1m25 308 11 ( ( -LRB- cord-013178-li1x1m25 308 12 pos pos NNP cord-013178-li1x1m25 308 13 ) ) -RRB- cord-013178-li1x1m25 308 14 control control NN cord-013178-li1x1m25 308 15 and and CC cord-013178-li1x1m25 308 16 a a DT cord-013178-li1x1m25 308 17 negative negative JJ cord-013178-li1x1m25 308 18 ( ( -LRB- cord-013178-li1x1m25 308 19 neg neg NNP cord-013178-li1x1m25 308 20 ) ) -RRB- cord-013178-li1x1m25 308 21 control control NN cord-013178-li1x1m25 308 22 , , , cord-013178-li1x1m25 308 23 respectively respectively RB cord-013178-li1x1m25 308 24 , , , cord-013178-li1x1m25 308 25 in in IN cord-013178-li1x1m25 308 26 the the DT cord-013178-li1x1m25 308 27 ORF3 ORF3 NNP cord-013178-li1x1m25 308 28 protein protein NN cord-013178-li1x1m25 308 29 ELISA ELISA NNP cord-013178-li1x1m25 308 30 . . . cord-013178-li1x1m25 309 1 The the DT cord-013178-li1x1m25 309 2 data datum NNS cord-013178-li1x1m25 309 3 represent represent VBP cord-013178-li1x1m25 309 4 the the DT cord-013178-li1x1m25 309 5 mean mean JJ cord-013178-li1x1m25 309 6 ± ± NNP cord-013178-li1x1m25 309 7 SE SE NNP cord-013178-li1x1m25 309 8 . . . cord-013178-li1x1m25 310 1 Treatments treatment NNS cord-013178-li1x1m25 310 2 with with IN cord-013178-li1x1m25 310 3 different different JJ cord-013178-li1x1m25 310 4 letters letter NNS cord-013178-li1x1m25 310 5 have have VBP cord-013178-li1x1m25 310 6 statistically statistically RB cord-013178-li1x1m25 310 7 significant significant JJ cord-013178-li1x1m25 310 8 differences difference NNS cord-013178-li1x1m25 310 9 ( ( -LRB- cord-013178-li1x1m25 310 10 p p NN cord-013178-li1x1m25 310 11 < < XX cord-013178-li1x1m25 310 12 0.05 0.05 CD cord-013178-li1x1m25 310 13 ) ) -RRB- cord-013178-li1x1m25 310 14 . . . cord-013178-li1x1m25 311 1 The the DT cord-013178-li1x1m25 311 2 anti anti JJ cord-013178-li1x1m25 311 3 - - JJ cord-013178-li1x1m25 311 4 viral viral JJ cord-013178-li1x1m25 311 5 protein protein NN cord-013178-li1x1m25 311 6 - - HYPH cord-013178-li1x1m25 311 7 specific specific JJ cord-013178-li1x1m25 311 8 antibodies antibody NNS cord-013178-li1x1m25 311 9 were be VBD cord-013178-li1x1m25 311 10 detected detect VBN cord-013178-li1x1m25 311 11 in in IN cord-013178-li1x1m25 311 12 the the DT cord-013178-li1x1m25 311 13 plasm plasm NN cord-013178-li1x1m25 311 14 samples sample NNS cord-013178-li1x1m25 311 15 using use VBG cord-013178-li1x1m25 311 16 iELISA iELISA NNP cord-013178-li1x1m25 311 17 techniques technique NNS cord-013178-li1x1m25 311 18 . . . cord-013178-li1x1m25 312 1 The the DT cord-013178-li1x1m25 312 2 cut cut VB cord-013178-li1x1m25 312 3 - - HYPH cord-013178-li1x1m25 312 4 off off RP cord-013178-li1x1m25 312 5 value value NN cord-013178-li1x1m25 312 6 of of IN cord-013178-li1x1m25 312 7 in in IN cord-013178-li1x1m25 312 8 the the DT cord-013178-li1x1m25 312 9 anti anti JJ cord-013178-li1x1m25 312 10 - - JJ cord-013178-li1x1m25 312 11 capsid capsid JJ cord-013178-li1x1m25 312 12 IgG igg NN cord-013178-li1x1m25 312 13 ELISA ELISA NNP cord-013178-li1x1m25 312 14 and and CC cord-013178-li1x1m25 312 15 the the DT cord-013178-li1x1m25 312 16 anti anti NNP cord-013178-li1x1m25 312 17 - - NNP cord-013178-li1x1m25 312 18 ORF3 ORF3 NNP cord-013178-li1x1m25 312 19 peptide peptide NN cord-013178-li1x1m25 312 20 ( ( -LRB- cord-013178-li1x1m25 312 21 P126 p126 CD cord-013178-li1x1m25 312 22 ) ) -RRB- cord-013178-li1x1m25 313 1 IgG IgG NNP cord-013178-li1x1m25 313 2 ELISA ELISA NNP cord-013178-li1x1m25 313 3 were be VBD cord-013178-li1x1m25 313 4 0.20 0.20 CD cord-013178-li1x1m25 313 5 and and CC cord-013178-li1x1m25 313 6 0.18 0.18 CD cord-013178-li1x1m25 313 7 , , , cord-013178-li1x1m25 313 8 respectively respectively RB cord-013178-li1x1m25 313 9 . . . cord-013178-li1x1m25 314 1 Non non JJ cord-013178-li1x1m25 314 2 - - JJ cord-013178-li1x1m25 314 3 vaccinated vaccinated JJ cord-013178-li1x1m25 314 4 suckers sucker NNS cord-013178-li1x1m25 314 5 showed show VBD cord-013178-li1x1m25 314 6 the the DT cord-013178-li1x1m25 314 7 highest high JJS cord-013178-li1x1m25 314 8 mean mean JJ cord-013178-li1x1m25 314 9 OD OD NNP cord-013178-li1x1m25 314 10 405 405 CD cord-013178-li1x1m25 314 11 value value NN cord-013178-li1x1m25 314 12 of of IN cord-013178-li1x1m25 314 13 anti anti JJ cord-013178-li1x1m25 314 14 - - JJ cord-013178-li1x1m25 314 15 capsid capsid JJ cord-013178-li1x1m25 314 16 IgG igg NN cord-013178-li1x1m25 314 17 and and CC cord-013178-li1x1m25 314 18 anti anti NNP cord-013178-li1x1m25 314 19 - - NNP cord-013178-li1x1m25 314 20 ORF3 orf3 JJ cord-013178-li1x1m25 314 21 IgG igg NN cord-013178-li1x1m25 314 22 at at IN cord-013178-li1x1m25 314 23 one one CD cord-013178-li1x1m25 314 24 week week NN cord-013178-li1x1m25 314 25 of of IN cord-013178-li1x1m25 314 26 age age NN cord-013178-li1x1m25 314 27 . . . cord-013178-li1x1m25 315 1 It -PRON- PRP cord-013178-li1x1m25 315 2 may may MD cord-013178-li1x1m25 315 3 mean mean VB cord-013178-li1x1m25 315 4 that that IN cord-013178-li1x1m25 315 5 non non JJ cord-013178-li1x1m25 315 6 - - JJ cord-013178-li1x1m25 315 7 vaccinated vaccinated JJ cord-013178-li1x1m25 315 8 suckers sucker NNS cord-013178-li1x1m25 315 9 absorbed absorb VBD cord-013178-li1x1m25 315 10 large large JJ cord-013178-li1x1m25 315 11 amounts amount NNS cord-013178-li1x1m25 315 12 of of IN cord-013178-li1x1m25 315 13 maternally maternally RB cord-013178-li1x1m25 315 14 derived derive VBN cord-013178-li1x1m25 315 15 antibodies antibody NNS cord-013178-li1x1m25 315 16 after after IN cord-013178-li1x1m25 315 17 birth birth NN cord-013178-li1x1m25 315 18 . . . cord-013178-li1x1m25 316 1 Notably notably RB cord-013178-li1x1m25 316 2 , , , cord-013178-li1x1m25 316 3 the the DT cord-013178-li1x1m25 316 4 non non JJ cord-013178-li1x1m25 316 5 - - JJ cord-013178-li1x1m25 316 6 vaccinated vaccinated JJ cord-013178-li1x1m25 316 7 group group NN cord-013178-li1x1m25 316 8 showed show VBD cord-013178-li1x1m25 316 9 the the DT cord-013178-li1x1m25 316 10 most most RBS cord-013178-li1x1m25 316 11 elevated elevated JJ cord-013178-li1x1m25 316 12 standard standard JJ cord-013178-li1x1m25 316 13 error error NN cord-013178-li1x1m25 316 14 of of IN cord-013178-li1x1m25 316 15 OD OD NNP cord-013178-li1x1m25 316 16 405 405 CD cord-013178-li1x1m25 316 17 value value NN cord-013178-li1x1m25 316 18 ( ( -LRB- cord-013178-li1x1m25 316 19 anti anti JJ cord-013178-li1x1m25 316 20 - - JJ cord-013178-li1x1m25 316 21 capsid capsid JJ cord-013178-li1x1m25 316 22 IgG igg NN cord-013178-li1x1m25 316 23 ) ) -RRB- cord-013178-li1x1m25 316 24 in in IN cord-013178-li1x1m25 316 25 this this DT cord-013178-li1x1m25 316 26 statistical statistical JJ cord-013178-li1x1m25 316 27 analysis analysis NN cord-013178-li1x1m25 316 28 . . . cord-013178-li1x1m25 317 1 A a DT cord-013178-li1x1m25 317 2 gradual gradual JJ cord-013178-li1x1m25 317 3 decrease decrease NN cord-013178-li1x1m25 317 4 of of IN cord-013178-li1x1m25 317 5 the the DT cord-013178-li1x1m25 317 6 mean mean NN cord-013178-li1x1m25 317 7 OD OD NNP cord-013178-li1x1m25 317 8 405 405 CD cord-013178-li1x1m25 317 9 value value NN cord-013178-li1x1m25 317 10 of of IN cord-013178-li1x1m25 317 11 anti anti JJ cord-013178-li1x1m25 317 12 - - JJ cord-013178-li1x1m25 317 13 capsid capsid JJ cord-013178-li1x1m25 317 14 IgG igg NN cord-013178-li1x1m25 317 15 for for IN cord-013178-li1x1m25 317 16 piglets piglet NNS cord-013178-li1x1m25 317 17 from from IN cord-013178-li1x1m25 317 18 the the DT cord-013178-li1x1m25 317 19 suckling suckling NN cord-013178-li1x1m25 317 20 period period NN cord-013178-li1x1m25 317 21 ( ( -LRB- cord-013178-li1x1m25 317 22 one one CD cord-013178-li1x1m25 317 23 week week NN cord-013178-li1x1m25 317 24 of of IN cord-013178-li1x1m25 317 25 age age NN cord-013178-li1x1m25 317 26 ) ) -RRB- cord-013178-li1x1m25 317 27 to to IN cord-013178-li1x1m25 317 28 the the DT cord-013178-li1x1m25 317 29 weaning weaning NN cord-013178-li1x1m25 317 30 period period NN cord-013178-li1x1m25 317 31 ( ( -LRB- cord-013178-li1x1m25 317 32 six six CD cord-013178-li1x1m25 317 33 weeks week NNS cord-013178-li1x1m25 317 34 of of IN cord-013178-li1x1m25 317 35 age age NN cord-013178-li1x1m25 317 36 ) ) -RRB- cord-013178-li1x1m25 317 37 was be VBD cord-013178-li1x1m25 317 38 observed observe VBN cord-013178-li1x1m25 317 39 . . . cord-013178-li1x1m25 318 1 From from IN cord-013178-li1x1m25 318 2 9 9 CD cord-013178-li1x1m25 318 3 to to TO cord-013178-li1x1m25 318 4 12 12 CD cord-013178-li1x1m25 318 5 weeks week NNS cord-013178-li1x1m25 318 6 old old JJ cord-013178-li1x1m25 318 7 , , , cord-013178-li1x1m25 318 8 the the DT cord-013178-li1x1m25 318 9 mean mean JJ cord-013178-li1x1m25 318 10 OD OD NNP cord-013178-li1x1m25 318 11 405 405 CD cord-013178-li1x1m25 318 12 value value NN cord-013178-li1x1m25 318 13 of of IN cord-013178-li1x1m25 318 14 anti anti JJ cord-013178-li1x1m25 318 15 - - JJ cord-013178-li1x1m25 318 16 capsid capsid JJ cord-013178-li1x1m25 318 17 IgG igg NN cord-013178-li1x1m25 318 18 increased increase VBD cord-013178-li1x1m25 318 19 significantly significantly RB cord-013178-li1x1m25 318 20 ( ( -LRB- cord-013178-li1x1m25 318 21 p p NN cord-013178-li1x1m25 318 22 < < XX cord-013178-li1x1m25 318 23 0.05 0.05 CD cord-013178-li1x1m25 318 24 ; ; : cord-013178-li1x1m25 318 25 Figure Figure NNP cord-013178-li1x1m25 318 26 6 6 CD cord-013178-li1x1m25 318 27 ) ) -RRB- cord-013178-li1x1m25 318 28 . . . cord-013178-li1x1m25 319 1 The the DT cord-013178-li1x1m25 319 2 mean mean NN cord-013178-li1x1m25 319 3 OD OD NNP cord-013178-li1x1m25 319 4 405 405 CD cord-013178-li1x1m25 319 5 value value NN cord-013178-li1x1m25 319 6 of of IN cord-013178-li1x1m25 319 7 anti anti JJ cord-013178-li1x1m25 319 8 - - JJ cord-013178-li1x1m25 319 9 capsid capsid JJ cord-013178-li1x1m25 319 10 IgG igg NN cord-013178-li1x1m25 319 11 also also RB cord-013178-li1x1m25 319 12 increased increase VBD cord-013178-li1x1m25 319 13 significantly significantly RB cord-013178-li1x1m25 319 14 for for IN cord-013178-li1x1m25 319 15 pigs pig NNS cord-013178-li1x1m25 319 16 from from IN cord-013178-li1x1m25 319 17 15 15 CD cord-013178-li1x1m25 319 18 to to TO cord-013178-li1x1m25 319 19 18 18 CD cord-013178-li1x1m25 319 20 weeks week NNS cord-013178-li1x1m25 319 21 old old JJ cord-013178-li1x1m25 320 1 ( ( -LRB- cord-013178-li1x1m25 320 2 p p NN cord-013178-li1x1m25 320 3 < < XX cord-013178-li1x1m25 320 4 0.05 0.05 CD cord-013178-li1x1m25 320 5 ) ) -RRB- cord-013178-li1x1m25 321 1 and and CC cord-013178-li1x1m25 321 2 reached reach VBD cord-013178-li1x1m25 321 3 a a DT cord-013178-li1x1m25 321 4 plateau plateau NN cord-013178-li1x1m25 321 5 at at IN cord-013178-li1x1m25 321 6 18 18 CD cord-013178-li1x1m25 321 7 weeks week NNS cord-013178-li1x1m25 321 8 old old JJ cord-013178-li1x1m25 321 9 . . . cord-013178-li1x1m25 322 1 The the DT cord-013178-li1x1m25 322 2 same same JJ cord-013178-li1x1m25 322 3 trend trend NN cord-013178-li1x1m25 322 4 was be VBD cord-013178-li1x1m25 322 5 observed observe VBN cord-013178-li1x1m25 322 6 in in IN cord-013178-li1x1m25 322 7 the the DT cord-013178-li1x1m25 322 8 result result NN cord-013178-li1x1m25 322 9 of of IN cord-013178-li1x1m25 322 10 the the DT cord-013178-li1x1m25 322 11 anti anti JJ cord-013178-li1x1m25 322 12 - - NNP cord-013178-li1x1m25 322 13 ORF3 ORF3 NNP cord-013178-li1x1m25 322 14 IgG igg NN cord-013178-li1x1m25 322 15 ELISA ELISA NNP cord-013178-li1x1m25 322 16 test test NN cord-013178-li1x1m25 322 17 , , , cord-013178-li1x1m25 322 18 except except IN cord-013178-li1x1m25 322 19 for for IN cord-013178-li1x1m25 322 20 OD OD NNP cord-013178-li1x1m25 322 21 405 405 CD cord-013178-li1x1m25 322 22 values value NNS cord-013178-li1x1m25 322 23 of of IN cord-013178-li1x1m25 322 24 anti anti JJ cord-013178-li1x1m25 322 25 - - JJ cord-013178-li1x1m25 322 26 ORF3 orf3 JJ cord-013178-li1x1m25 322 27 IgG igg NN cord-013178-li1x1m25 322 28 were be VBD cord-013178-li1x1m25 322 29 lower low JJR cord-013178-li1x1m25 322 30 than than IN cord-013178-li1x1m25 322 31 anti anti JJ cord-013178-li1x1m25 322 32 - - JJ cord-013178-li1x1m25 322 33 capsid capsid JJ cord-013178-li1x1m25 323 1 IgG. IgG. NNP cord-013178-li1x1m25 324 1 Notwithstanding notwithstanding IN cord-013178-li1x1m25 324 2 , , , cord-013178-li1x1m25 324 3 it -PRON- PRP cord-013178-li1x1m25 324 4 is be VBZ cord-013178-li1x1m25 324 5 worth worth JJ cord-013178-li1x1m25 324 6 mentioning mention VBG cord-013178-li1x1m25 324 7 that that IN cord-013178-li1x1m25 324 8 there there EX cord-013178-li1x1m25 324 9 was be VBD cord-013178-li1x1m25 324 10 a a DT cord-013178-li1x1m25 324 11 sharp sharp JJ cord-013178-li1x1m25 324 12 decrease decrease NN cord-013178-li1x1m25 324 13 of of IN cord-013178-li1x1m25 324 14 the the DT cord-013178-li1x1m25 324 15 mean mean NN cord-013178-li1x1m25 324 16 OD OD NNP cord-013178-li1x1m25 324 17 405 405 CD cord-013178-li1x1m25 324 18 value value NN cord-013178-li1x1m25 324 19 of of IN cord-013178-li1x1m25 324 20 anti anti JJ cord-013178-li1x1m25 324 21 - - JJ cord-013178-li1x1m25 324 22 ORF3 orf3 JJ cord-013178-li1x1m25 324 23 IgG igg NN cord-013178-li1x1m25 324 24 for for IN cord-013178-li1x1m25 324 25 suckers sucker NNS cord-013178-li1x1m25 324 26 from from IN cord-013178-li1x1m25 324 27 one one CD cord-013178-li1x1m25 324 28 to to TO cord-013178-li1x1m25 324 29 three three CD cord-013178-li1x1m25 324 30 weeks week NNS cord-013178-li1x1m25 324 31 old old JJ cord-013178-li1x1m25 324 32 . . . cord-013178-li1x1m25 325 1 The the DT cord-013178-li1x1m25 325 2 mean mean NN cord-013178-li1x1m25 325 3 OD OD NNP cord-013178-li1x1m25 325 4 405 405 CD cord-013178-li1x1m25 325 5 value value NN cord-013178-li1x1m25 325 6 of of IN cord-013178-li1x1m25 325 7 anti anti JJ cord-013178-li1x1m25 325 8 - - JJ cord-013178-li1x1m25 325 9 ORF3 ORF3 NNP cord-013178-li1x1m25 325 10 IgG igg NN cord-013178-li1x1m25 325 11 increased increase VBD cord-013178-li1x1m25 325 12 significantly significantly RB cord-013178-li1x1m25 325 13 for for IN cord-013178-li1x1m25 325 14 pigs pig NNS cord-013178-li1x1m25 325 15 from from IN cord-013178-li1x1m25 325 16 six six CD cord-013178-li1x1m25 325 17 to to TO cord-013178-li1x1m25 325 18 nine nine CD cord-013178-li1x1m25 325 19 weeks week NNS cord-013178-li1x1m25 325 20 old old JJ cord-013178-li1x1m25 326 1 ( ( -LRB- cord-013178-li1x1m25 326 2 p p NN cord-013178-li1x1m25 326 3 < < XX cord-013178-li1x1m25 326 4 0.05 0.05 CD cord-013178-li1x1m25 326 5 ; ; : cord-013178-li1x1m25 326 6 Figure Figure NNP cord-013178-li1x1m25 326 7 6 6 CD cord-013178-li1x1m25 326 8 ) ) -RRB- cord-013178-li1x1m25 326 9 and and CC cord-013178-li1x1m25 326 10 reached reach VBD cord-013178-li1x1m25 326 11 a a DT cord-013178-li1x1m25 326 12 plateau plateau NN cord-013178-li1x1m25 326 13 at at IN cord-013178-li1x1m25 326 14 nine nine CD cord-013178-li1x1m25 326 15 weeks week NNS cord-013178-li1x1m25 326 16 old old JJ cord-013178-li1x1m25 326 17 . . . cord-013178-li1x1m25 327 1 There there EX cord-013178-li1x1m25 327 2 was be VBD cord-013178-li1x1m25 327 3 a a DT cord-013178-li1x1m25 327 4 weak weak JJ cord-013178-li1x1m25 327 5 correlation correlation NN cord-013178-li1x1m25 327 6 ( ( -LRB- cord-013178-li1x1m25 327 7 r r NN cord-013178-li1x1m25 327 8 = = SYM cord-013178-li1x1m25 327 9 0.3771 0.3771 CD cord-013178-li1x1m25 327 10 ) ) -RRB- cord-013178-li1x1m25 327 11 between between IN cord-013178-li1x1m25 327 12 the the DT cord-013178-li1x1m25 327 13 OD OD NNP cord-013178-li1x1m25 327 14 405 405 CD cord-013178-li1x1m25 327 15 value value NN cord-013178-li1x1m25 327 16 of of IN cord-013178-li1x1m25 327 17 anti anti JJ cord-013178-li1x1m25 327 18 - - JJ cord-013178-li1x1m25 327 19 capsid capsid JJ cord-013178-li1x1m25 327 20 IgG igg NN cord-013178-li1x1m25 327 21 and and CC cord-013178-li1x1m25 327 22 the the DT cord-013178-li1x1m25 327 23 OD OD NNP cord-013178-li1x1m25 327 24 405 405 CD cord-013178-li1x1m25 327 25 value value NN cord-013178-li1x1m25 327 26 of of IN cord-013178-li1x1m25 327 27 anti anti JJ cord-013178-li1x1m25 327 28 - - JJ cord-013178-li1x1m25 327 29 ORF3 orf3 JJ cord-013178-li1x1m25 327 30 IgG igg NN cord-013178-li1x1m25 327 31 in in IN cord-013178-li1x1m25 327 32 the the DT cord-013178-li1x1m25 327 33 plasm plasm NN cord-013178-li1x1m25 327 34 samples sample NNS cord-013178-li1x1m25 327 35 . . . cord-013178-li1x1m25 328 1 It -PRON- PRP cord-013178-li1x1m25 328 2 points point VBZ cord-013178-li1x1m25 328 3 to to IN cord-013178-li1x1m25 328 4 the the DT cord-013178-li1x1m25 328 5 probability probability NN cord-013178-li1x1m25 328 6 that that IN cord-013178-li1x1m25 328 7 the the DT cord-013178-li1x1m25 328 8 amount amount NN cord-013178-li1x1m25 328 9 of of IN cord-013178-li1x1m25 328 10 anti anti JJ cord-013178-li1x1m25 328 11 - - JJ cord-013178-li1x1m25 328 12 capsid capsid JJ cord-013178-li1x1m25 328 13 IgG igg NN cord-013178-li1x1m25 328 14 reflects reflect VBZ cord-013178-li1x1m25 328 15 humoral humoral JJ cord-013178-li1x1m25 328 16 immunity immunity NN cord-013178-li1x1m25 328 17 in in IN cord-013178-li1x1m25 328 18 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 328 19 pigs pig NNS cord-013178-li1x1m25 328 20 before before IN cord-013178-li1x1m25 328 21 and and CC cord-013178-li1x1m25 328 22 after after IN cord-013178-li1x1m25 328 23 the the DT cord-013178-li1x1m25 328 24 injection injection NN cord-013178-li1x1m25 328 25 of of IN cord-013178-li1x1m25 328 26 a a DT cord-013178-li1x1m25 328 27 PCV2 PCV2 NNP cord-013178-li1x1m25 328 28 vaccine vaccine NN cord-013178-li1x1m25 328 29 . . . cord-013178-li1x1m25 329 1 Besides besides RB cord-013178-li1x1m25 329 2 , , , cord-013178-li1x1m25 329 3 the the DT cord-013178-li1x1m25 329 4 amount amount NN cord-013178-li1x1m25 329 5 of of IN cord-013178-li1x1m25 329 6 anti anti JJ cord-013178-li1x1m25 329 7 - - JJ cord-013178-li1x1m25 329 8 ORF3 orf3 JJ cord-013178-li1x1m25 329 9 IgG igg NN cord-013178-li1x1m25 329 10 reflects reflect VBZ cord-013178-li1x1m25 329 11 humoral humoral JJ cord-013178-li1x1m25 329 12 immunity immunity NN cord-013178-li1x1m25 329 13 in in IN cord-013178-li1x1m25 329 14 pigs pig NNS cord-013178-li1x1m25 329 15 , , , cord-013178-li1x1m25 329 16 which which WDT cord-013178-li1x1m25 329 17 were be VBD cord-013178-li1x1m25 329 18 caused cause VBN cord-013178-li1x1m25 329 19 by by IN cord-013178-li1x1m25 329 20 PCV2 PCV2 NNP cord-013178-li1x1m25 329 21 infecting infect VBG cord-013178-li1x1m25 329 22 only only RB cord-013178-li1x1m25 329 23 . . . cord-013178-li1x1m25 330 1 The the DT cord-013178-li1x1m25 330 2 mean mean NN cord-013178-li1x1m25 330 3 OD405 OD405 NNP cord-013178-li1x1m25 330 4 value value NN cord-013178-li1x1m25 330 5 of of IN cord-013178-li1x1m25 330 6 anti anti JJ cord-013178-li1x1m25 330 7 - - JJ cord-013178-li1x1m25 330 8 capsid capsid JJ cord-013178-li1x1m25 330 9 IgG igg NN cord-013178-li1x1m25 330 10 also also RB cord-013178-li1x1m25 330 11 increased increase VBD cord-013178-li1x1m25 330 12 significantly significantly RB cord-013178-li1x1m25 330 13 for for IN cord-013178-li1x1m25 330 14 pigs pig NNS cord-013178-li1x1m25 330 15 from from IN cord-013178-li1x1m25 330 16 15 15 CD cord-013178-li1x1m25 330 17 to to TO cord-013178-li1x1m25 330 18 18 18 CD cord-013178-li1x1m25 330 19 weeks week NNS cord-013178-li1x1m25 330 20 old old JJ cord-013178-li1x1m25 331 1 ( ( -LRB- cord-013178-li1x1m25 331 2 p p NN cord-013178-li1x1m25 331 3 < < XX cord-013178-li1x1m25 331 4 0.05 0.05 CD cord-013178-li1x1m25 331 5 ) ) -RRB- cord-013178-li1x1m25 332 1 and and CC cord-013178-li1x1m25 332 2 reached reach VBD cord-013178-li1x1m25 332 3 a a DT cord-013178-li1x1m25 332 4 plateau plateau NN cord-013178-li1x1m25 332 5 at at IN cord-013178-li1x1m25 332 6 18 18 CD cord-013178-li1x1m25 332 7 weeks week NNS cord-013178-li1x1m25 332 8 old old JJ cord-013178-li1x1m25 332 9 . . . cord-013178-li1x1m25 333 1 The the DT cord-013178-li1x1m25 333 2 same same JJ cord-013178-li1x1m25 333 3 trend trend NN cord-013178-li1x1m25 333 4 was be VBD cord-013178-li1x1m25 333 5 observed observe VBN cord-013178-li1x1m25 333 6 in in IN cord-013178-li1x1m25 333 7 the the DT cord-013178-li1x1m25 333 8 result result NN cord-013178-li1x1m25 333 9 of of IN cord-013178-li1x1m25 333 10 the the DT cord-013178-li1x1m25 333 11 anti anti JJ cord-013178-li1x1m25 333 12 - - NNP cord-013178-li1x1m25 333 13 ORF3 ORF3 NNP cord-013178-li1x1m25 333 14 IgG igg NN cord-013178-li1x1m25 333 15 ELISA ELISA NNP cord-013178-li1x1m25 333 16 test test NN cord-013178-li1x1m25 333 17 , , , cord-013178-li1x1m25 333 18 except except IN cord-013178-li1x1m25 333 19 for for IN cord-013178-li1x1m25 333 20 OD405 od405 JJ cord-013178-li1x1m25 333 21 values value NNS cord-013178-li1x1m25 333 22 of of IN cord-013178-li1x1m25 333 23 anti anti JJ cord-013178-li1x1m25 333 24 - - JJ cord-013178-li1x1m25 333 25 ORF3 orf3 JJ cord-013178-li1x1m25 333 26 IgG igg NN cord-013178-li1x1m25 333 27 were be VBD cord-013178-li1x1m25 333 28 lower low JJR cord-013178-li1x1m25 333 29 than than IN cord-013178-li1x1m25 333 30 anti anti JJ cord-013178-li1x1m25 333 31 - - JJ cord-013178-li1x1m25 333 32 capsid capsid JJ cord-013178-li1x1m25 334 1 IgG. IgG. NNP cord-013178-li1x1m25 335 1 Notwithstanding notwithstanding IN cord-013178-li1x1m25 335 2 , , , cord-013178-li1x1m25 335 3 it -PRON- PRP cord-013178-li1x1m25 335 4 is be VBZ cord-013178-li1x1m25 335 5 worth worth JJ cord-013178-li1x1m25 335 6 mentioning mention VBG cord-013178-li1x1m25 335 7 that that IN cord-013178-li1x1m25 335 8 there there EX cord-013178-li1x1m25 335 9 was be VBD cord-013178-li1x1m25 335 10 a a DT cord-013178-li1x1m25 335 11 sharp sharp JJ cord-013178-li1x1m25 335 12 decrease decrease NN cord-013178-li1x1m25 335 13 of of IN cord-013178-li1x1m25 335 14 the the DT cord-013178-li1x1m25 335 15 mean mean NN cord-013178-li1x1m25 335 16 OD405 OD405 NNP cord-013178-li1x1m25 335 17 value value NN cord-013178-li1x1m25 335 18 of of IN cord-013178-li1x1m25 335 19 anti anti JJ cord-013178-li1x1m25 335 20 - - JJ cord-013178-li1x1m25 335 21 ORF3 orf3 JJ cord-013178-li1x1m25 335 22 IgG igg NN cord-013178-li1x1m25 335 23 for for IN cord-013178-li1x1m25 335 24 suckers sucker NNS cord-013178-li1x1m25 335 25 from from IN cord-013178-li1x1m25 335 26 one one CD cord-013178-li1x1m25 335 27 to to TO cord-013178-li1x1m25 335 28 three three CD cord-013178-li1x1m25 335 29 weeks week NNS cord-013178-li1x1m25 335 30 old old JJ cord-013178-li1x1m25 335 31 . . . cord-013178-li1x1m25 336 1 The the DT cord-013178-li1x1m25 336 2 mean mean NN cord-013178-li1x1m25 336 3 OD405 OD405 NNP cord-013178-li1x1m25 336 4 value value NN cord-013178-li1x1m25 336 5 of of IN cord-013178-li1x1m25 336 6 anti anti JJ cord-013178-li1x1m25 336 7 - - JJ cord-013178-li1x1m25 336 8 ORF3 ORF3 NNP cord-013178-li1x1m25 336 9 IgG igg NN cord-013178-li1x1m25 336 10 increased increase VBD cord-013178-li1x1m25 336 11 significantly significantly RB cord-013178-li1x1m25 336 12 for for IN cord-013178-li1x1m25 336 13 pigs pig NNS cord-013178-li1x1m25 336 14 from from IN cord-013178-li1x1m25 336 15 six six CD cord-013178-li1x1m25 336 16 to to TO cord-013178-li1x1m25 336 17 nine nine CD cord-013178-li1x1m25 336 18 weeks week NNS cord-013178-li1x1m25 336 19 old old JJ cord-013178-li1x1m25 337 1 ( ( -LRB- cord-013178-li1x1m25 337 2 p p NN cord-013178-li1x1m25 337 3 < < XX cord-013178-li1x1m25 337 4 0.05 0.05 CD cord-013178-li1x1m25 337 5 ; ; : cord-013178-li1x1m25 337 6 Figure Figure NNP cord-013178-li1x1m25 337 7 6 6 CD cord-013178-li1x1m25 337 8 ) ) -RRB- cord-013178-li1x1m25 337 9 and and CC cord-013178-li1x1m25 337 10 reached reach VBD cord-013178-li1x1m25 337 11 a a DT cord-013178-li1x1m25 337 12 plateau plateau NN cord-013178-li1x1m25 337 13 at at IN cord-013178-li1x1m25 337 14 nine nine CD cord-013178-li1x1m25 337 15 weeks week NNS cord-013178-li1x1m25 337 16 old old JJ cord-013178-li1x1m25 337 17 . . . cord-013178-li1x1m25 338 1 There there EX cord-013178-li1x1m25 338 2 was be VBD cord-013178-li1x1m25 338 3 a a DT cord-013178-li1x1m25 338 4 weak weak JJ cord-013178-li1x1m25 338 5 correlation correlation NN cord-013178-li1x1m25 338 6 ( ( -LRB- cord-013178-li1x1m25 338 7 r r NN cord-013178-li1x1m25 338 8 = = SYM cord-013178-li1x1m25 338 9 0.3771 0.3771 CD cord-013178-li1x1m25 338 10 ) ) -RRB- cord-013178-li1x1m25 338 11 between between IN cord-013178-li1x1m25 338 12 the the DT cord-013178-li1x1m25 338 13 OD405 OD405 NNP cord-013178-li1x1m25 338 14 value value NN cord-013178-li1x1m25 338 15 of of IN cord-013178-li1x1m25 338 16 anti anti JJ cord-013178-li1x1m25 338 17 - - JJ cord-013178-li1x1m25 338 18 capsid capsid JJ cord-013178-li1x1m25 338 19 IgG igg NN cord-013178-li1x1m25 338 20 and and CC cord-013178-li1x1m25 338 21 the the DT cord-013178-li1x1m25 338 22 OD405 OD405 NNP cord-013178-li1x1m25 338 23 value value NN cord-013178-li1x1m25 338 24 of of IN cord-013178-li1x1m25 338 25 anti anti JJ cord-013178-li1x1m25 338 26 - - JJ cord-013178-li1x1m25 338 27 ORF3 orf3 JJ cord-013178-li1x1m25 338 28 IgG igg NN cord-013178-li1x1m25 338 29 in in IN cord-013178-li1x1m25 338 30 the the DT cord-013178-li1x1m25 338 31 plasm plasm NN cord-013178-li1x1m25 338 32 samples sample NNS cord-013178-li1x1m25 338 33 . . . cord-013178-li1x1m25 339 1 It -PRON- PRP cord-013178-li1x1m25 339 2 points point VBZ cord-013178-li1x1m25 339 3 to to IN cord-013178-li1x1m25 339 4 the the DT cord-013178-li1x1m25 339 5 probability probability NN cord-013178-li1x1m25 339 6 that that IN cord-013178-li1x1m25 339 7 the the DT cord-013178-li1x1m25 339 8 amount amount NN cord-013178-li1x1m25 339 9 of of IN cord-013178-li1x1m25 339 10 anti anti JJ cord-013178-li1x1m25 339 11 - - JJ cord-013178-li1x1m25 339 12 capsid capsid JJ cord-013178-li1x1m25 339 13 IgG igg NN cord-013178-li1x1m25 339 14 reflects reflect VBZ cord-013178-li1x1m25 339 15 humoral humoral JJ cord-013178-li1x1m25 339 16 immunity immunity NN cord-013178-li1x1m25 339 17 in in IN cord-013178-li1x1m25 339 18 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 339 19 pigs pig NNS cord-013178-li1x1m25 339 20 before before IN cord-013178-li1x1m25 339 21 and and CC cord-013178-li1x1m25 339 22 after after IN cord-013178-li1x1m25 339 23 the the DT cord-013178-li1x1m25 339 24 injection injection NN cord-013178-li1x1m25 339 25 of of IN cord-013178-li1x1m25 339 26 a a DT cord-013178-li1x1m25 339 27 PCV2 PCV2 NNP cord-013178-li1x1m25 339 28 vaccine vaccine NN cord-013178-li1x1m25 339 29 . . . cord-013178-li1x1m25 340 1 Besides besides RB cord-013178-li1x1m25 340 2 , , , cord-013178-li1x1m25 340 3 the the DT cord-013178-li1x1m25 340 4 amount amount NN cord-013178-li1x1m25 340 5 of of IN cord-013178-li1x1m25 340 6 anti anti JJ cord-013178-li1x1m25 340 7 - - JJ cord-013178-li1x1m25 340 8 ORF3 orf3 JJ cord-013178-li1x1m25 340 9 IgG igg NN cord-013178-li1x1m25 340 10 reflects reflect VBZ cord-013178-li1x1m25 340 11 humoral humoral JJ cord-013178-li1x1m25 340 12 immunity immunity NN cord-013178-li1x1m25 340 13 in in IN cord-013178-li1x1m25 340 14 pigs pig NNS cord-013178-li1x1m25 340 15 , , , cord-013178-li1x1m25 340 16 which which WDT cord-013178-li1x1m25 340 17 were be VBD cord-013178-li1x1m25 340 18 caused cause VBN cord-013178-li1x1m25 340 19 by by IN cord-013178-li1x1m25 340 20 PCV2 PCV2 NNP cord-013178-li1x1m25 340 21 infecting infect VBG cord-013178-li1x1m25 340 22 only only RB cord-013178-li1x1m25 340 23 . . . cord-013178-li1x1m25 341 1 Figure figure NN cord-013178-li1x1m25 341 2 6 6 CD cord-013178-li1x1m25 341 3 . . . cord-013178-li1x1m25 342 1 Assessment assessment NN cord-013178-li1x1m25 342 2 of of IN cord-013178-li1x1m25 342 3 the the DT cord-013178-li1x1m25 342 4 anti anti JJ cord-013178-li1x1m25 342 5 - - JJ cord-013178-li1x1m25 342 6 capsid capsid JJ cord-013178-li1x1m25 342 7 IgG igg NN cord-013178-li1x1m25 342 8 and and CC cord-013178-li1x1m25 342 9 the the DT cord-013178-li1x1m25 342 10 anti anti NNP cord-013178-li1x1m25 342 11 - - NNP cord-013178-li1x1m25 342 12 ORF3 ORF3 NNP cord-013178-li1x1m25 342 13 peptide peptide NN cord-013178-li1x1m25 342 14 ( ( -LRB- cord-013178-li1x1m25 342 15 P126 p126 CD cord-013178-li1x1m25 342 16 ) ) -RRB- cord-013178-li1x1m25 342 17 in in IN cord-013178-li1x1m25 342 18 the the DT cord-013178-li1x1m25 342 19 plasm plasm NN cord-013178-li1x1m25 342 20 samples sample NNS cord-013178-li1x1m25 342 21 from from IN cord-013178-li1x1m25 342 22 pigs pig NNS cord-013178-li1x1m25 342 23 of of IN cord-013178-li1x1m25 342 24 different different JJ cord-013178-li1x1m25 342 25 age age NN cord-013178-li1x1m25 342 26 groups group NNS cord-013178-li1x1m25 342 27 . . . cord-013178-li1x1m25 343 1 The the DT cord-013178-li1x1m25 343 2 experiments experiment NNS cord-013178-li1x1m25 343 3 were be VBD cord-013178-li1x1m25 343 4 repeated repeat VBN cord-013178-li1x1m25 343 5 three three CD cord-013178-li1x1m25 343 6 times time NNS cord-013178-li1x1m25 343 7 , , , cord-013178-li1x1m25 343 8 and and CC cord-013178-li1x1m25 343 9 data datum NNS cord-013178-li1x1m25 343 10 represent represent VBP cord-013178-li1x1m25 343 11 the the DT cord-013178-li1x1m25 343 12 mean mean JJ cord-013178-li1x1m25 343 13 ± ± NNP cord-013178-li1x1m25 343 14 SE SE NNP cord-013178-li1x1m25 343 15 . . . cord-013178-li1x1m25 344 1 Treatments treatment NNS cord-013178-li1x1m25 344 2 with with IN cord-013178-li1x1m25 344 3 different different JJ cord-013178-li1x1m25 344 4 letters letter NNS cord-013178-li1x1m25 344 5 at at IN cord-013178-li1x1m25 344 6 the the DT cord-013178-li1x1m25 344 7 same same JJ cord-013178-li1x1m25 344 8 kind kind NN cord-013178-li1x1m25 344 9 of of IN cord-013178-li1x1m25 344 10 antibody antibody NN cord-013178-li1x1m25 344 11 assay assay NN cord-013178-li1x1m25 344 12 have have VBP cord-013178-li1x1m25 344 13 statistically statistically RB cord-013178-li1x1m25 344 14 significant significant JJ cord-013178-li1x1m25 344 15 differences difference NNS cord-013178-li1x1m25 344 16 ( ( -LRB- cord-013178-li1x1m25 344 17 p p NN cord-013178-li1x1m25 344 18 < < XX cord-013178-li1x1m25 344 19 0.05 0.05 CD cord-013178-li1x1m25 344 20 ) ) -RRB- cord-013178-li1x1m25 344 21 . . . cord-013178-li1x1m25 345 1 According accord VBG cord-013178-li1x1m25 345 2 to to IN cord-013178-li1x1m25 345 3 a a DT cord-013178-li1x1m25 345 4 previous previous JJ cord-013178-li1x1m25 345 5 study study NN cord-013178-li1x1m25 345 6 , , , cord-013178-li1x1m25 345 7 the the DT cord-013178-li1x1m25 345 8 ORF3 ORF3 NNP cord-013178-li1x1m25 345 9 peptide peptide NN cord-013178-li1x1m25 345 10 ( ( -LRB- cord-013178-li1x1m25 345 11 residues residue NNS cord-013178-li1x1m25 345 12 35 35 CD cord-013178-li1x1m25 345 13 - - SYM cord-013178-li1x1m25 345 14 65 65 CD cord-013178-li1x1m25 345 15 ) ) -RRB- cord-013178-li1x1m25 345 16 of of IN cord-013178-li1x1m25 345 17 PCV2 PCV2 NNP cord-013178-li1x1m25 345 18 interacts interact VBZ cord-013178-li1x1m25 345 19 with with IN cord-013178-li1x1m25 345 20 the the DT cord-013178-li1x1m25 345 21 p53-binding p53-binding JJ cord-013178-li1x1m25 345 22 domain domain NN cord-013178-li1x1m25 345 23 of of IN cord-013178-li1x1m25 345 24 pPirh2 ppirh2 FW cord-013178-li1x1m25 345 25 [ [ -LRB- cord-013178-li1x1m25 345 26 42 42 CD cord-013178-li1x1m25 345 27 ] ] -RRB- cord-013178-li1x1m25 345 28 . . . cord-013178-li1x1m25 346 1 The the DT cord-013178-li1x1m25 346 2 peptide peptide NN cord-013178-li1x1m25 346 3 N1 n1 NN cord-013178-li1x1m25 346 4 ( ( -LRB- cord-013178-li1x1m25 346 5 residues residue NNS cord-013178-li1x1m25 346 6 35 35 CD cord-013178-li1x1m25 346 7 - - SYM cord-013178-li1x1m25 346 8 66 66 CD cord-013178-li1x1m25 346 9 ) ) -RRB- cord-013178-li1x1m25 346 10 of of IN cord-013178-li1x1m25 346 11 the the DT cord-013178-li1x1m25 346 12 ORF3 ORF3 NNP cord-013178-li1x1m25 346 13 protein protein NN cord-013178-li1x1m25 346 14 was be VBD cord-013178-li1x1m25 346 15 synthesized synthesize VBN cord-013178-li1x1m25 346 16 and and CC cord-013178-li1x1m25 346 17 used use VBN cord-013178-li1x1m25 346 18 in in IN cord-013178-li1x1m25 346 19 this this DT cord-013178-li1x1m25 346 20 study study NN cord-013178-li1x1m25 346 21 . . . cord-013178-li1x1m25 347 1 Subsequently subsequently RB cord-013178-li1x1m25 347 2 , , , cord-013178-li1x1m25 347 3 anti anti NNP cord-013178-li1x1m25 347 4 - - NNP cord-013178-li1x1m25 347 5 ORF3 ORF3 NNP cord-013178-li1x1m25 347 6 mAbs mAbs NNP cord-013178-li1x1m25 347 7 were be VBD cord-013178-li1x1m25 347 8 generated generate VBN cord-013178-li1x1m25 347 9 and and CC cord-013178-li1x1m25 347 10 characterized characterize VBN cord-013178-li1x1m25 347 11 in in IN cord-013178-li1x1m25 347 12 this this DT cord-013178-li1x1m25 347 13 study study NN cord-013178-li1x1m25 347 14 . . . cord-013178-li1x1m25 348 1 This this DT cord-013178-li1x1m25 348 2 work work NN cord-013178-li1x1m25 348 3 created create VBD cord-013178-li1x1m25 348 4 one one CD cord-013178-li1x1m25 348 5 hybridoma hybridoma NN cord-013178-li1x1m25 348 6 producing produce VBG cord-013178-li1x1m25 348 7 the the DT cord-013178-li1x1m25 348 8 mAb mAb NNP cord-013178-li1x1m25 348 9 7D3 7D3 NNP cord-013178-li1x1m25 348 10 with with IN cord-013178-li1x1m25 348 11 IgG1 IgG1 NNP cord-013178-li1x1m25 348 12 , , , cord-013178-li1x1m25 348 13 and and CC cord-013178-li1x1m25 348 14 the the DT cord-013178-li1x1m25 348 15 other other JJ cord-013178-li1x1m25 348 16 producing produce VBG cord-013178-li1x1m25 348 17 the the DT cord-013178-li1x1m25 348 18 defective defective JJ cord-013178-li1x1m25 349 1 -Ig -ig LS cord-013178-li1x1m25 349 2 mAb mAb NNS cord-013178-li1x1m25 349 3 ( ( -LRB- cord-013178-li1x1m25 349 4 6D10 6d10 CD cord-013178-li1x1m25 349 5 ) ) -RRB- cord-013178-li1x1m25 350 1 with with IN cord-013178-li1x1m25 350 2 a a DT cord-013178-li1x1m25 350 3 lambda lambda NN cord-013178-li1x1m25 350 4 - - HYPH cord-013178-li1x1m25 350 5 light light JJ cord-013178-li1x1m25 350 6 chain chain NN cord-013178-li1x1m25 350 7 . . . cord-013178-li1x1m25 351 1 The the DT cord-013178-li1x1m25 351 2 mAb mAb NNP cord-013178-li1x1m25 351 3 7D3 7D3 NNP cord-013178-li1x1m25 351 4 ( ( -LRB- cord-013178-li1x1m25 351 5 IgG1 igg1 NN cord-013178-li1x1m25 351 6 ) ) -RRB- cord-013178-li1x1m25 351 7 bound bind VBN cord-013178-li1x1m25 351 8 to to IN cord-013178-li1x1m25 351 9 the the DT cord-013178-li1x1m25 351 10 linear linear JJ cord-013178-li1x1m25 351 11 peptide peptide NN cord-013178-li1x1m25 351 12 N1 n1 NN cord-013178-li1x1m25 351 13 ( ( -LRB- cord-013178-li1x1m25 351 14 C35HNDVYISLPI C35HNDVYISLPI NNP cord-013178-li1x1m25 351 15 TLLHFPAHFQ TLLHFPAHFQ NNP cord-013178-li1x1m25 351 16 KFSQPAEISDKR66 kfsqpaeisdkr66 XX cord-013178-li1x1m25 351 17 ) ) -RRB- cord-013178-li1x1m25 351 18 and and CC cord-013178-li1x1m25 351 19 P126 P126 NNP cord-013178-li1x1m25 351 20 ( ( -LRB- cord-013178-li1x1m25 351 21 35HNDVYISLPI 35hndvyislpi CD cord-013178-li1x1m25 351 22 TLLHFPAHFQ TLLHFPAHFQ NNP cord-013178-li1x1m25 351 23 KFSQPAEISDKR66 kfsqpaeisdkr66 XX cord-013178-li1x1m25 351 24 ) ) -RRB- cord-013178-li1x1m25 351 25 . . . cord-013178-li1x1m25 352 1 However however RB cord-013178-li1x1m25 352 2 , , , cord-013178-li1x1m25 352 3 the the DT cord-013178-li1x1m25 352 4 defective defective JJ cord-013178-li1x1m25 352 5 - - HYPH cord-013178-li1x1m25 352 6 Ig ig NN cord-013178-li1x1m25 352 7 mAb mAb NNS cord-013178-li1x1m25 352 8 6D10 6d10 CD cord-013178-li1x1m25 352 9 minorly minorly RB cord-013178-li1x1m25 352 10 reacted react VBD cord-013178-li1x1m25 352 11 with with IN cord-013178-li1x1m25 352 12 all all DT cord-013178-li1x1m25 352 13 truncated truncate VBN cord-013178-li1x1m25 352 14 peptides peptide NNS cord-013178-li1x1m25 352 15 . . . cord-013178-li1x1m25 353 1 This this DT cord-013178-li1x1m25 353 2 finding finding NN cord-013178-li1x1m25 353 3 is be VBZ cord-013178-li1x1m25 353 4 similar similar JJ cord-013178-li1x1m25 353 5 to to IN cord-013178-li1x1m25 353 6 the the DT cord-013178-li1x1m25 353 7 previous previous JJ cord-013178-li1x1m25 353 8 study study NN cord-013178-li1x1m25 353 9 [ [ -LRB- cord-013178-li1x1m25 353 10 49 49 CD cord-013178-li1x1m25 353 11 ] ] -RRB- cord-013178-li1x1m25 353 12 . . . cord-013178-li1x1m25 354 1 The the DT cord-013178-li1x1m25 354 2 defective defective JJ cord-013178-li1x1m25 354 3 - - HYPH cord-013178-li1x1m25 354 4 Ig Ig NNP cord-013178-li1x1m25 354 5 mAbs mAbs NNP cord-013178-li1x1m25 354 6 likely likely RB cord-013178-li1x1m25 354 7 have have VB cord-013178-li1x1m25 354 8 broad broad JJ cord-013178-li1x1m25 354 9 binding binding NN cord-013178-li1x1m25 354 10 , , , cord-013178-li1x1m25 354 11 moderate moderate JJ cord-013178-li1x1m25 354 12 specificity specificity NN cord-013178-li1x1m25 354 13 , , , cord-013178-li1x1m25 354 14 and and CC cord-013178-li1x1m25 354 15 low low JJ cord-013178-li1x1m25 354 16 affinity affinity NN cord-013178-li1x1m25 354 17 with with IN cord-013178-li1x1m25 354 18 the the DT cord-013178-li1x1m25 354 19 associated associated JJ cord-013178-li1x1m25 354 20 peptide peptide NN cord-013178-li1x1m25 354 21 . . . cord-013178-li1x1m25 355 1 There there EX cord-013178-li1x1m25 355 2 were be VBD cord-013178-li1x1m25 355 3 concerns concern NNS cord-013178-li1x1m25 355 4 about about IN cord-013178-li1x1m25 355 5 this this DT cord-013178-li1x1m25 355 6 defective defective JJ cord-013178-li1x1m25 355 7 - - HYPH cord-013178-li1x1m25 355 8 Ig ig NN cord-013178-li1x1m25 355 9 mAb mAb NNS cord-013178-li1x1m25 355 10 since since IN cord-013178-li1x1m25 355 11 this this DT cord-013178-li1x1m25 355 12 seems seem VBZ cord-013178-li1x1m25 355 13 to to TO cord-013178-li1x1m25 355 14 be be VB cord-013178-li1x1m25 355 15 very very RB cord-013178-li1x1m25 355 16 rare rare JJ cord-013178-li1x1m25 355 17 . . . cord-013178-li1x1m25 356 1 Further further RB cord-013178-li1x1m25 356 2 , , , cord-013178-li1x1m25 356 3 the the DT cord-013178-li1x1m25 356 4 molecular molecular JJ cord-013178-li1x1m25 356 5 weight weight NN cord-013178-li1x1m25 356 6 of of IN cord-013178-li1x1m25 356 7 the the DT cord-013178-li1x1m25 356 8 mAb mAb NNS cord-013178-li1x1m25 356 9 6D10 6d10 CD cord-013178-li1x1m25 356 10 was be VBD cord-013178-li1x1m25 356 11 determined determine VBN cord-013178-li1x1m25 356 12 by by IN cord-013178-li1x1m25 356 13 Western western JJ cord-013178-li1x1m25 356 14 blotting blotting NN cord-013178-li1x1m25 356 15 . . . cord-013178-li1x1m25 357 1 Two two CD cord-013178-li1x1m25 357 2 bands band NNS cord-013178-li1x1m25 357 3 were be VBD cord-013178-li1x1m25 357 4 observed observe VBN cord-013178-li1x1m25 357 5 Figure Figure NNP cord-013178-li1x1m25 357 6 6 6 CD cord-013178-li1x1m25 357 7 . . . cord-013178-li1x1m25 358 1 Assessment assessment NN cord-013178-li1x1m25 358 2 of of IN cord-013178-li1x1m25 358 3 the the DT cord-013178-li1x1m25 358 4 anti anti JJ cord-013178-li1x1m25 358 5 - - JJ cord-013178-li1x1m25 358 6 capsid capsid JJ cord-013178-li1x1m25 358 7 IgG igg NN cord-013178-li1x1m25 358 8 and and CC cord-013178-li1x1m25 358 9 the the DT cord-013178-li1x1m25 358 10 anti anti NNP cord-013178-li1x1m25 358 11 - - NNP cord-013178-li1x1m25 358 12 ORF3 ORF3 NNP cord-013178-li1x1m25 358 13 peptide peptide NN cord-013178-li1x1m25 358 14 ( ( -LRB- cord-013178-li1x1m25 358 15 P126 p126 CD cord-013178-li1x1m25 358 16 ) ) -RRB- cord-013178-li1x1m25 358 17 in in IN cord-013178-li1x1m25 358 18 the the DT cord-013178-li1x1m25 358 19 plasm plasm NN cord-013178-li1x1m25 358 20 samples sample NNS cord-013178-li1x1m25 358 21 from from IN cord-013178-li1x1m25 358 22 pigs pig NNS cord-013178-li1x1m25 358 23 of of IN cord-013178-li1x1m25 358 24 different different JJ cord-013178-li1x1m25 358 25 age age NN cord-013178-li1x1m25 358 26 groups group NNS cord-013178-li1x1m25 358 27 . . . cord-013178-li1x1m25 359 1 The the DT cord-013178-li1x1m25 359 2 experiments experiment NNS cord-013178-li1x1m25 359 3 were be VBD cord-013178-li1x1m25 359 4 repeated repeat VBN cord-013178-li1x1m25 359 5 three three CD cord-013178-li1x1m25 359 6 times time NNS cord-013178-li1x1m25 359 7 , , , cord-013178-li1x1m25 359 8 and and CC cord-013178-li1x1m25 359 9 data datum NNS cord-013178-li1x1m25 359 10 represent represent VBP cord-013178-li1x1m25 359 11 the the DT cord-013178-li1x1m25 359 12 mean mean JJ cord-013178-li1x1m25 359 13 ± ± NNP cord-013178-li1x1m25 359 14 SE SE NNP cord-013178-li1x1m25 359 15 . . . cord-013178-li1x1m25 360 1 Treatments treatment NNS cord-013178-li1x1m25 360 2 with with IN cord-013178-li1x1m25 360 3 different different JJ cord-013178-li1x1m25 360 4 letters letter NNS cord-013178-li1x1m25 360 5 at at IN cord-013178-li1x1m25 360 6 the the DT cord-013178-li1x1m25 360 7 same same JJ cord-013178-li1x1m25 360 8 kind kind NN cord-013178-li1x1m25 360 9 of of IN cord-013178-li1x1m25 360 10 antibody antibody NN cord-013178-li1x1m25 360 11 assay assay NN cord-013178-li1x1m25 360 12 have have VBP cord-013178-li1x1m25 360 13 statistically statistically RB cord-013178-li1x1m25 360 14 significant significant JJ cord-013178-li1x1m25 360 15 differences difference NNS cord-013178-li1x1m25 360 16 ( ( -LRB- cord-013178-li1x1m25 360 17 p p NN cord-013178-li1x1m25 360 18 < < XX cord-013178-li1x1m25 360 19 0.05 0.05 CD cord-013178-li1x1m25 360 20 ) ) -RRB- cord-013178-li1x1m25 360 21 . . . cord-013178-li1x1m25 361 1 According accord VBG cord-013178-li1x1m25 361 2 to to IN cord-013178-li1x1m25 361 3 a a DT cord-013178-li1x1m25 361 4 previous previous JJ cord-013178-li1x1m25 361 5 study study NN cord-013178-li1x1m25 361 6 , , , cord-013178-li1x1m25 361 7 the the DT cord-013178-li1x1m25 361 8 ORF3 ORF3 NNP cord-013178-li1x1m25 361 9 peptide peptide NN cord-013178-li1x1m25 361 10 ( ( -LRB- cord-013178-li1x1m25 361 11 residues residue NNS cord-013178-li1x1m25 361 12 35 35 CD cord-013178-li1x1m25 361 13 - - SYM cord-013178-li1x1m25 361 14 65 65 CD cord-013178-li1x1m25 361 15 ) ) -RRB- cord-013178-li1x1m25 361 16 of of IN cord-013178-li1x1m25 361 17 PCV2 PCV2 NNP cord-013178-li1x1m25 361 18 interacts interact VBZ cord-013178-li1x1m25 361 19 with with IN cord-013178-li1x1m25 361 20 the the DT cord-013178-li1x1m25 361 21 p53-binding p53-binding JJ cord-013178-li1x1m25 361 22 domain domain NN cord-013178-li1x1m25 361 23 of of IN cord-013178-li1x1m25 361 24 pPirh2 ppirh2 FW cord-013178-li1x1m25 361 25 [ [ -LRB- cord-013178-li1x1m25 361 26 42 42 CD cord-013178-li1x1m25 361 27 ] ] -RRB- cord-013178-li1x1m25 361 28 . . . cord-013178-li1x1m25 362 1 The the DT cord-013178-li1x1m25 362 2 peptide peptide NN cord-013178-li1x1m25 362 3 N1 n1 NN cord-013178-li1x1m25 362 4 ( ( -LRB- cord-013178-li1x1m25 362 5 residues residue NNS cord-013178-li1x1m25 362 6 35 35 CD cord-013178-li1x1m25 362 7 - - SYM cord-013178-li1x1m25 362 8 66 66 CD cord-013178-li1x1m25 362 9 ) ) -RRB- cord-013178-li1x1m25 362 10 of of IN cord-013178-li1x1m25 362 11 the the DT cord-013178-li1x1m25 362 12 ORF3 ORF3 NNP cord-013178-li1x1m25 362 13 protein protein NN cord-013178-li1x1m25 362 14 was be VBD cord-013178-li1x1m25 362 15 synthesized synthesize VBN cord-013178-li1x1m25 362 16 and and CC cord-013178-li1x1m25 362 17 used use VBN cord-013178-li1x1m25 362 18 in in IN cord-013178-li1x1m25 362 19 this this DT cord-013178-li1x1m25 362 20 study study NN cord-013178-li1x1m25 362 21 . . . cord-013178-li1x1m25 363 1 Subsequently subsequently RB cord-013178-li1x1m25 363 2 , , , cord-013178-li1x1m25 363 3 anti anti NNP cord-013178-li1x1m25 363 4 - - NNP cord-013178-li1x1m25 363 5 ORF3 ORF3 NNP cord-013178-li1x1m25 363 6 mAbs mAbs NNP cord-013178-li1x1m25 363 7 were be VBD cord-013178-li1x1m25 363 8 generated generate VBN cord-013178-li1x1m25 363 9 and and CC cord-013178-li1x1m25 363 10 characterized characterize VBN cord-013178-li1x1m25 363 11 in in IN cord-013178-li1x1m25 363 12 this this DT cord-013178-li1x1m25 363 13 study study NN cord-013178-li1x1m25 363 14 . . . cord-013178-li1x1m25 364 1 This this DT cord-013178-li1x1m25 364 2 work work NN cord-013178-li1x1m25 364 3 created create VBD cord-013178-li1x1m25 364 4 one one CD cord-013178-li1x1m25 364 5 hybridoma hybridoma NN cord-013178-li1x1m25 364 6 producing produce VBG cord-013178-li1x1m25 364 7 the the DT cord-013178-li1x1m25 364 8 mAb mAb NNP cord-013178-li1x1m25 364 9 7D3 7D3 NNP cord-013178-li1x1m25 364 10 with with IN cord-013178-li1x1m25 364 11 IgG1 IgG1 NNP cord-013178-li1x1m25 364 12 , , , cord-013178-li1x1m25 364 13 and and CC cord-013178-li1x1m25 364 14 the the DT cord-013178-li1x1m25 364 15 other other JJ cord-013178-li1x1m25 364 16 producing produce VBG cord-013178-li1x1m25 364 17 the the DT cord-013178-li1x1m25 364 18 defective defective JJ cord-013178-li1x1m25 365 1 -Ig -ig LS cord-013178-li1x1m25 365 2 mAb mAb NNS cord-013178-li1x1m25 365 3 ( ( -LRB- cord-013178-li1x1m25 365 4 6D10 6d10 CD cord-013178-li1x1m25 365 5 ) ) -RRB- cord-013178-li1x1m25 366 1 with with IN cord-013178-li1x1m25 366 2 a a DT cord-013178-li1x1m25 366 3 lambda lambda NN cord-013178-li1x1m25 366 4 - - HYPH cord-013178-li1x1m25 366 5 light light JJ cord-013178-li1x1m25 366 6 chain chain NN cord-013178-li1x1m25 366 7 . . . cord-013178-li1x1m25 367 1 The the DT cord-013178-li1x1m25 367 2 mAb mAb NNP cord-013178-li1x1m25 367 3 7D3 7D3 NNP cord-013178-li1x1m25 367 4 ( ( -LRB- cord-013178-li1x1m25 367 5 IgG1 igg1 NN cord-013178-li1x1m25 367 6 ) ) -RRB- cord-013178-li1x1m25 367 7 bound bind VBN cord-013178-li1x1m25 367 8 to to IN cord-013178-li1x1m25 367 9 the the DT cord-013178-li1x1m25 367 10 linear linear JJ cord-013178-li1x1m25 367 11 peptide peptide NN cord-013178-li1x1m25 367 12 N1 n1 NN cord-013178-li1x1m25 367 13 ( ( -LRB- cord-013178-li1x1m25 367 14 C C NNP cord-013178-li1x1m25 367 15 35 35 CD cord-013178-li1x1m25 367 16 HNDVYISLPI HNDVYISLPI NNP cord-013178-li1x1m25 367 17 TLLHFPAHFQ TLLHFPAHFQ NNP cord-013178-li1x1m25 367 18 KFSQPAEISDKR KFSQPAEISDKR NNP cord-013178-li1x1m25 367 19 66 66 CD cord-013178-li1x1m25 367 20 ) ) -RRB- cord-013178-li1x1m25 367 21 and and CC cord-013178-li1x1m25 367 22 P126 P126 NNP cord-013178-li1x1m25 367 23 ( ( -LRB- cord-013178-li1x1m25 367 24 35 35 CD cord-013178-li1x1m25 367 25 HNDVYISLPI HNDVYISLPI NNP cord-013178-li1x1m25 367 26 TLLHFPAHFQ TLLHFPAHFQ NNP cord-013178-li1x1m25 367 27 KFSQPAEISDKR KFSQPAEISDKR NNP cord-013178-li1x1m25 367 28 66 66 CD cord-013178-li1x1m25 367 29 ) ) -RRB- cord-013178-li1x1m25 367 30 . . . cord-013178-li1x1m25 368 1 However however RB cord-013178-li1x1m25 368 2 , , , cord-013178-li1x1m25 368 3 the the DT cord-013178-li1x1m25 368 4 defective defective JJ cord-013178-li1x1m25 368 5 - - HYPH cord-013178-li1x1m25 368 6 Ig ig NN cord-013178-li1x1m25 368 7 mAb mAb NNS cord-013178-li1x1m25 368 8 6D10 6d10 CD cord-013178-li1x1m25 368 9 minorly minorly RB cord-013178-li1x1m25 368 10 reacted react VBD cord-013178-li1x1m25 368 11 with with IN cord-013178-li1x1m25 368 12 all all DT cord-013178-li1x1m25 368 13 truncated truncate VBN cord-013178-li1x1m25 368 14 peptides peptide NNS cord-013178-li1x1m25 368 15 . . . cord-013178-li1x1m25 369 1 This this DT cord-013178-li1x1m25 369 2 finding finding NN cord-013178-li1x1m25 369 3 is be VBZ cord-013178-li1x1m25 369 4 similar similar JJ cord-013178-li1x1m25 369 5 to to IN cord-013178-li1x1m25 369 6 the the DT cord-013178-li1x1m25 369 7 previous previous JJ cord-013178-li1x1m25 369 8 study study NN cord-013178-li1x1m25 369 9 [ [ -LRB- cord-013178-li1x1m25 369 10 49 49 CD cord-013178-li1x1m25 369 11 ] ] -RRB- cord-013178-li1x1m25 369 12 . . . cord-013178-li1x1m25 370 1 The the DT cord-013178-li1x1m25 370 2 defective defective JJ cord-013178-li1x1m25 370 3 - - HYPH cord-013178-li1x1m25 370 4 Ig Ig NNP cord-013178-li1x1m25 370 5 mAbs mAbs NNP cord-013178-li1x1m25 370 6 likely likely RB cord-013178-li1x1m25 370 7 have have VB cord-013178-li1x1m25 370 8 broad broad JJ cord-013178-li1x1m25 370 9 binding binding NN cord-013178-li1x1m25 370 10 , , , cord-013178-li1x1m25 370 11 moderate moderate JJ cord-013178-li1x1m25 370 12 specificity specificity NN cord-013178-li1x1m25 370 13 , , , cord-013178-li1x1m25 370 14 and and CC cord-013178-li1x1m25 370 15 low low JJ cord-013178-li1x1m25 370 16 affinity affinity NN cord-013178-li1x1m25 370 17 with with IN cord-013178-li1x1m25 370 18 the the DT cord-013178-li1x1m25 370 19 associated associated JJ cord-013178-li1x1m25 370 20 peptide peptide NN cord-013178-li1x1m25 370 21 . . . cord-013178-li1x1m25 371 1 There there EX cord-013178-li1x1m25 371 2 were be VBD cord-013178-li1x1m25 371 3 concerns concern NNS cord-013178-li1x1m25 371 4 about about IN cord-013178-li1x1m25 371 5 this this DT cord-013178-li1x1m25 371 6 defective defective JJ cord-013178-li1x1m25 371 7 - - HYPH cord-013178-li1x1m25 371 8 Ig ig NN cord-013178-li1x1m25 371 9 mAb mAb NNS cord-013178-li1x1m25 371 10 since since IN cord-013178-li1x1m25 371 11 this this DT cord-013178-li1x1m25 371 12 seems seem VBZ cord-013178-li1x1m25 371 13 to to TO cord-013178-li1x1m25 371 14 be be VB cord-013178-li1x1m25 371 15 very very RB cord-013178-li1x1m25 371 16 rare rare JJ cord-013178-li1x1m25 371 17 . . . cord-013178-li1x1m25 372 1 Further further RB cord-013178-li1x1m25 372 2 , , , cord-013178-li1x1m25 372 3 the the DT cord-013178-li1x1m25 372 4 molecular molecular JJ cord-013178-li1x1m25 372 5 weight weight NN cord-013178-li1x1m25 372 6 of of IN cord-013178-li1x1m25 372 7 the the DT cord-013178-li1x1m25 372 8 mAb mAb NNS cord-013178-li1x1m25 372 9 6D10 6d10 CD cord-013178-li1x1m25 372 10 was be VBD cord-013178-li1x1m25 372 11 determined determine VBN cord-013178-li1x1m25 372 12 by by IN cord-013178-li1x1m25 372 13 Western western JJ cord-013178-li1x1m25 372 14 blotting blotting NN cord-013178-li1x1m25 372 15 . . . cord-013178-li1x1m25 373 1 Two two CD cord-013178-li1x1m25 373 2 bands band NNS cord-013178-li1x1m25 373 3 were be VBD cord-013178-li1x1m25 373 4 observed observe VBN cord-013178-li1x1m25 373 5 at at IN cord-013178-li1x1m25 373 6 about about RB cord-013178-li1x1m25 373 7 33 33 CD cord-013178-li1x1m25 373 8 and and CC cord-013178-li1x1m25 373 9 6 6 CD cord-013178-li1x1m25 373 10 kDa kDa NNS cord-013178-li1x1m25 373 11 , , , cord-013178-li1x1m25 373 12 respectively respectively RB cord-013178-li1x1m25 373 13 . . . cord-013178-li1x1m25 374 1 The the DT cord-013178-li1x1m25 374 2 small small JJ cord-013178-li1x1m25 374 3 one one NN cord-013178-li1x1m25 374 4 ( ( -LRB- cord-013178-li1x1m25 374 5 6 6 CD cord-013178-li1x1m25 374 6 kDa kDa NNPS cord-013178-li1x1m25 374 7 ) ) -RRB- cord-013178-li1x1m25 374 8 implied imply VBD cord-013178-li1x1m25 374 9 some some DT cord-013178-li1x1m25 374 10 degradation degradation NN cord-013178-li1x1m25 374 11 of of IN cord-013178-li1x1m25 374 12 this this DT cord-013178-li1x1m25 374 13 mAb mab NN cord-013178-li1x1m25 374 14 . . . cord-013178-li1x1m25 375 1 Since since IN cord-013178-li1x1m25 375 2 the the DT cord-013178-li1x1m25 375 3 molecular molecular JJ cord-013178-li1x1m25 375 4 weight weight NN cord-013178-li1x1m25 375 5 of of IN cord-013178-li1x1m25 375 6 the the DT cord-013178-li1x1m25 375 7 light light JJ cord-013178-li1x1m25 375 8 chain chain NN cord-013178-li1x1m25 375 9 was be VBD cord-013178-li1x1m25 375 10 25 25 CD cord-013178-li1x1m25 375 11 kDa kDa NNS cord-013178-li1x1m25 375 12 , , , cord-013178-li1x1m25 375 13 the the DT cord-013178-li1x1m25 375 14 author author NN cord-013178-li1x1m25 375 15 suggested suggest VBD cord-013178-li1x1m25 375 16 that that IN cord-013178-li1x1m25 375 17 the the DT cord-013178-li1x1m25 375 18 mAb mAb NNS cord-013178-li1x1m25 375 19 6D10 6d10 CD cord-013178-li1x1m25 375 20 might may MD cord-013178-li1x1m25 375 21 contain contain VB cord-013178-li1x1m25 375 22 a a DT cord-013178-li1x1m25 375 23 short short JJ cord-013178-li1x1m25 375 24 fragment fragment NN cord-013178-li1x1m25 375 25 of of IN cord-013178-li1x1m25 375 26 a a DT cord-013178-li1x1m25 375 27 protein protein NN cord-013178-li1x1m25 375 28 . . . cord-013178-li1x1m25 376 1 Interestingly interestingly RB cord-013178-li1x1m25 376 2 , , , cord-013178-li1x1m25 376 3 the the DT cord-013178-li1x1m25 376 4 author author NN cord-013178-li1x1m25 376 5 also also RB cord-013178-li1x1m25 376 6 tested test VBD cord-013178-li1x1m25 376 7 the the DT cord-013178-li1x1m25 376 8 molecular molecular JJ cord-013178-li1x1m25 376 9 weight weight NN cord-013178-li1x1m25 376 10 of of IN cord-013178-li1x1m25 376 11 another another DT cord-013178-li1x1m25 376 12 defective defective JJ cord-013178-li1x1m25 376 13 - - HYPH cord-013178-li1x1m25 376 14 Ig ig NN cord-013178-li1x1m25 376 15 mAb mAb NNS cord-013178-li1x1m25 376 16 6C3 6c3 CD cord-013178-li1x1m25 376 17 [ [ -LRB- cord-013178-li1x1m25 376 18 49 49 CD cord-013178-li1x1m25 376 19 ] ] -RRB- cord-013178-li1x1m25 376 20 ( ( -LRB- cord-013178-li1x1m25 376 21 which which WDT cord-013178-li1x1m25 376 22 against against IN cord-013178-li1x1m25 376 23 peptide peptide NN cord-013178-li1x1m25 376 24 C3 C3 NNP cord-013178-li1x1m25 376 25 ) ) -RRB- cord-013178-li1x1m25 376 26 , , , cord-013178-li1x1m25 376 27 and and CC cord-013178-li1x1m25 376 28 two two CD cord-013178-li1x1m25 376 29 molecules molecule NNS cord-013178-li1x1m25 376 30 at at IN cord-013178-li1x1m25 376 31 about about RB cord-013178-li1x1m25 376 32 33 33 CD cord-013178-li1x1m25 376 33 and and CC cord-013178-li1x1m25 376 34 6 6 CD cord-013178-li1x1m25 376 35 kDa kDa NNS cord-013178-li1x1m25 376 36 were be VBD cord-013178-li1x1m25 376 37 again again RB cord-013178-li1x1m25 376 38 shown show VBN cord-013178-li1x1m25 376 39 ( ( -LRB- cord-013178-li1x1m25 376 40 data datum NNS cord-013178-li1x1m25 376 41 not not RB cord-013178-li1x1m25 376 42 shown show VBN cord-013178-li1x1m25 376 43 ) ) -RRB- cord-013178-li1x1m25 376 44 . . . cord-013178-li1x1m25 377 1 According accord VBG cord-013178-li1x1m25 377 2 to to IN cord-013178-li1x1m25 377 3 previous previous JJ cord-013178-li1x1m25 377 4 documents document NNS cord-013178-li1x1m25 377 5 , , , cord-013178-li1x1m25 377 6 human human JJ cord-013178-li1x1m25 377 7 patients patient NNS cord-013178-li1x1m25 377 8 with with IN cord-013178-li1x1m25 377 9 abnormal abnormal JJ cord-013178-li1x1m25 377 10 serum serum NN cord-013178-li1x1m25 377 11 immunoglobulin immunoglobulin NN cord-013178-li1x1m25 377 12 - - HYPH cord-013178-li1x1m25 377 13 free free JJ cord-013178-li1x1m25 377 14 light light JJ cord-013178-li1x1m25 377 15 chain chain NN cord-013178-li1x1m25 377 16 production production NN cord-013178-li1x1m25 377 17 should should MD cord-013178-li1x1m25 377 18 only only RB cord-013178-li1x1m25 377 19 be be VB cord-013178-li1x1m25 377 20 due due JJ cord-013178-li1x1m25 377 21 to to IN cord-013178-li1x1m25 377 22 monoclonal monoclonal JJ cord-013178-li1x1m25 377 23 plasmaproliferative plasmaproliferative JJ cord-013178-li1x1m25 377 24 disorders disorder NNS cord-013178-li1x1m25 377 25 ( ( -LRB- cord-013178-li1x1m25 377 26 included include VBD cord-013178-li1x1m25 377 27 multiple multiple JJ cord-013178-li1x1m25 377 28 myeloma myeloma NN cord-013178-li1x1m25 377 29 , , , cord-013178-li1x1m25 377 30 light light JJ cord-013178-li1x1m25 377 31 chain chain NN cord-013178-li1x1m25 377 32 myelomas myeloma NNS cord-013178-li1x1m25 377 33 , , , cord-013178-li1x1m25 377 34 and and CC cord-013178-li1x1m25 377 35 light light JJ cord-013178-li1x1m25 377 36 chain chain NN cord-013178-li1x1m25 377 37 amyloidosis amyloidosis NN cord-013178-li1x1m25 377 38 ) ) -RRB- cord-013178-li1x1m25 377 39 [ [ -LRB- cord-013178-li1x1m25 377 40 51 51 CD cord-013178-li1x1m25 377 41 ] ] -RRB- cord-013178-li1x1m25 377 42 [ [ -LRB- cord-013178-li1x1m25 377 43 52 52 CD cord-013178-li1x1m25 377 44 ] ] -RRB- cord-013178-li1x1m25 378 1 [ [ -LRB- cord-013178-li1x1m25 378 2 53 53 CD cord-013178-li1x1m25 378 3 ] ] -RRB- cord-013178-li1x1m25 378 4 . . . cord-013178-li1x1m25 379 1 Arguably arguably RB cord-013178-li1x1m25 379 2 , , , cord-013178-li1x1m25 379 3 the the DT cord-013178-li1x1m25 379 4 defective defective JJ cord-013178-li1x1m25 379 5 - - HYPH cord-013178-li1x1m25 379 6 Ig ig NN cord-013178-li1x1m25 379 7 mAb mab NN cord-013178-li1x1m25 379 8 ( ( -LRB- cord-013178-li1x1m25 379 9 6D10 6d10 CD cord-013178-li1x1m25 379 10 ) ) -RRB- cord-013178-li1x1m25 379 11 might may MD cord-013178-li1x1m25 379 12 be be VB cord-013178-li1x1m25 379 13 related relate VBN cord-013178-li1x1m25 379 14 to to IN cord-013178-li1x1m25 379 15 the the DT cord-013178-li1x1m25 379 16 phenotype phenotype NN cord-013178-li1x1m25 379 17 of of IN cord-013178-li1x1m25 379 18 splenocytes splenocytes NNP cord-013178-li1x1m25 379 19 , , , cord-013178-li1x1m25 379 20 which which WDT cord-013178-li1x1m25 379 21 fused fuse VBD cord-013178-li1x1m25 379 22 with with IN cord-013178-li1x1m25 379 23 the the DT cord-013178-li1x1m25 379 24 myeloma myeloma NN cord-013178-li1x1m25 379 25 cell cell NN cord-013178-li1x1m25 379 26 . . . cord-013178-li1x1m25 380 1 Further further JJ cord-013178-li1x1m25 380 2 research research NN cord-013178-li1x1m25 380 3 should should MD cord-013178-li1x1m25 380 4 be be VB cord-013178-li1x1m25 380 5 carried carry VBN cord-013178-li1x1m25 380 6 out out RP cord-013178-li1x1m25 380 7 using use VBG cord-013178-li1x1m25 380 8 advanced advanced JJ cord-013178-li1x1m25 380 9 techniques technique NNS cord-013178-li1x1m25 380 10 ( ( -LRB- cord-013178-li1x1m25 380 11 such such JJ cord-013178-li1x1m25 380 12 as as IN cord-013178-li1x1m25 380 13 liquid liquid JJ cord-013178-li1x1m25 380 14 chromatography chromatography NN cord-013178-li1x1m25 380 15 with with IN cord-013178-li1x1m25 380 16 mass mass NN cord-013178-li1x1m25 380 17 spectrometry spectrometry NN cord-013178-li1x1m25 380 18 and and CC cord-013178-li1x1m25 380 19 spectroscopic spectroscopic JJ cord-013178-li1x1m25 380 20 techniques technique NNS cord-013178-li1x1m25 380 21 ) ) -RRB- cord-013178-li1x1m25 380 22 to to TO cord-013178-li1x1m25 380 23 investigate investigate VB cord-013178-li1x1m25 380 24 the the DT cord-013178-li1x1m25 380 25 constitution constitution NN cord-013178-li1x1m25 380 26 of of IN cord-013178-li1x1m25 380 27 the the DT cord-013178-li1x1m25 380 28 mAb mAb NNP cord-013178-li1x1m25 380 29 6D10 6d10 CD cord-013178-li1x1m25 380 30 . . . cord-013178-li1x1m25 381 1 Based base VBN cord-013178-li1x1m25 381 2 on on IN cord-013178-li1x1m25 381 3 previous previous JJ cord-013178-li1x1m25 381 4 reports report NNS cord-013178-li1x1m25 381 5 , , , cord-013178-li1x1m25 381 6 lymphocyte lymphocyte NN cord-013178-li1x1m25 381 7 depletion depletion NN cord-013178-li1x1m25 381 8 and and CC cord-013178-li1x1m25 381 9 apoptosis apoptosis NN cord-013178-li1x1m25 381 10 in in IN cord-013178-li1x1m25 381 11 lymphoid lymphoid JJ cord-013178-li1x1m25 381 12 tissues tissue NNS cord-013178-li1x1m25 381 13 are be VBP cord-013178-li1x1m25 381 14 histological histological JJ cord-013178-li1x1m25 381 15 hallmarks hallmark NNS cord-013178-li1x1m25 381 16 in in IN cord-013178-li1x1m25 381 17 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 381 18 pigs pig NNS cord-013178-li1x1m25 381 19 [ [ -LRB- cord-013178-li1x1m25 381 20 54 54 CD cord-013178-li1x1m25 381 21 ] ] -RRB- cord-013178-li1x1m25 382 1 [ [ -LRB- cord-013178-li1x1m25 382 2 55 55 CD cord-013178-li1x1m25 382 3 ] ] -RRB- cord-013178-li1x1m25 382 4 [ [ -LRB- cord-013178-li1x1m25 382 5 56 56 CD cord-013178-li1x1m25 382 6 ] ] -RRB- cord-013178-li1x1m25 382 7 . . . cord-013178-li1x1m25 383 1 Interestingly interestingly RB cord-013178-li1x1m25 383 2 , , , cord-013178-li1x1m25 383 3 these these DT cord-013178-li1x1m25 383 4 histopathological histopathological JJ cord-013178-li1x1m25 383 5 lesions lesion NNS cord-013178-li1x1m25 383 6 are be VBP cord-013178-li1x1m25 383 7 similar similar JJ cord-013178-li1x1m25 383 8 to to IN cord-013178-li1x1m25 383 9 Marek Marek NNP cord-013178-li1x1m25 383 10 's 's POS cord-013178-li1x1m25 383 11 disease disease NN cord-013178-li1x1m25 383 12 and and CC cord-013178-li1x1m25 383 13 African african JJ cord-013178-li1x1m25 383 14 swine swine NN cord-013178-li1x1m25 383 15 fever fever NN cord-013178-li1x1m25 383 16 [ [ -LRB- cord-013178-li1x1m25 383 17 57 57 CD cord-013178-li1x1m25 383 18 , , , cord-013178-li1x1m25 383 19 58 58 CD cord-013178-li1x1m25 383 20 ] ] -RRB- cord-013178-li1x1m25 383 21 . . . cord-013178-li1x1m25 384 1 More More JJR cord-013178-li1x1m25 384 2 recent recent JJ cord-013178-li1x1m25 384 3 evidence evidence NN cord-013178-li1x1m25 384 4 shows show VBZ cord-013178-li1x1m25 384 5 that that IN cord-013178-li1x1m25 384 6 these these DT cord-013178-li1x1m25 384 7 viruses virus NNS cord-013178-li1x1m25 384 8 caused cause VBD cord-013178-li1x1m25 384 9 an an DT cord-013178-li1x1m25 384 10 early early JJ cord-013178-li1x1m25 384 11 cytolytic cytolytic JJ cord-013178-li1x1m25 384 12 infection infection NN cord-013178-li1x1m25 384 13 in in IN cord-013178-li1x1m25 384 14 lymphocytes lymphocyte NNS cord-013178-li1x1m25 384 15 by by IN cord-013178-li1x1m25 384 16 apoptosis apoptosis NN cord-013178-li1x1m25 384 17 [ [ -LRB- cord-013178-li1x1m25 384 18 57 57 CD cord-013178-li1x1m25 384 19 ] ] -RRB- cord-013178-li1x1m25 384 20 [ [ -LRB- cord-013178-li1x1m25 384 21 58 58 CD cord-013178-li1x1m25 384 22 ] ] -RRB- cord-013178-li1x1m25 384 23 [ [ -LRB- cord-013178-li1x1m25 384 24 59 59 CD cord-013178-li1x1m25 384 25 ] ] -RRB- cord-013178-li1x1m25 384 26 . . . cord-013178-li1x1m25 385 1 Therefore therefore RB cord-013178-li1x1m25 385 2 , , , cord-013178-li1x1m25 385 3 this this DT cord-013178-li1x1m25 385 4 study study NN cord-013178-li1x1m25 385 5 hypothesized hypothesize VBD cord-013178-li1x1m25 385 6 that that IN cord-013178-li1x1m25 385 7 lymphocyte lymphocyte NN cord-013178-li1x1m25 385 8 and and CC cord-013178-li1x1m25 385 9 PBMCs pbmcs JJ cord-013178-li1x1m25 385 10 lineage lineage NN cord-013178-li1x1m25 385 11 cells cell NNS cord-013178-li1x1m25 385 12 should should MD cord-013178-li1x1m25 385 13 be be VB cord-013178-li1x1m25 385 14 the the DT cord-013178-li1x1m25 385 15 primary primary JJ cord-013178-li1x1m25 385 16 target target NN cord-013178-li1x1m25 385 17 cells cell NNS cord-013178-li1x1m25 385 18 for for IN cord-013178-li1x1m25 385 19 PCV2 pcv2 JJ cord-013178-li1x1m25 385 20 infection infection NN cord-013178-li1x1m25 385 21 . . . cord-013178-li1x1m25 386 1 To to IN cord-013178-li1x1m25 386 2 the the DT cord-013178-li1x1m25 386 3 best good JJS cord-013178-li1x1m25 386 4 of of IN cord-013178-li1x1m25 386 5 the the DT cord-013178-li1x1m25 386 6 author author NN cord-013178-li1x1m25 386 7 's 's POS cord-013178-li1x1m25 386 8 knowledge knowledge NN cord-013178-li1x1m25 386 9 , , , cord-013178-li1x1m25 386 10 the the DT cord-013178-li1x1m25 386 11 study study NN cord-013178-li1x1m25 386 12 of of IN cord-013178-li1x1m25 386 13 the the DT cord-013178-li1x1m25 386 14 ORF3 ORF3 NNP cord-013178-li1x1m25 386 15 protein protein NN cord-013178-li1x1m25 386 16 by by IN cord-013178-li1x1m25 386 17 indirect indirect JJ cord-013178-li1x1m25 386 18 immunofluorescence immunofluorescence NN cord-013178-li1x1m25 386 19 assay assay NN cord-013178-li1x1m25 386 20 in in IN cord-013178-li1x1m25 386 21 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 386 22 PBMCs pbmc NNS cord-013178-li1x1m25 386 23 has have VBZ cord-013178-li1x1m25 386 24 never never RB cord-013178-li1x1m25 386 25 been be VBN cord-013178-li1x1m25 386 26 performed perform VBN cord-013178-li1x1m25 386 27 , , , cord-013178-li1x1m25 386 28 and and CC cord-013178-li1x1m25 386 29 only only RB cord-013178-li1x1m25 386 30 a a DT cord-013178-li1x1m25 386 31 single single JJ cord-013178-li1x1m25 386 32 article article NN cord-013178-li1x1m25 386 33 mentions mention VBZ cord-013178-li1x1m25 386 34 the the DT cord-013178-li1x1m25 386 35 transient transient JJ cord-013178-li1x1m25 386 36 expression expression NN cord-013178-li1x1m25 386 37 of of IN cord-013178-li1x1m25 386 38 the the DT cord-013178-li1x1m25 386 39 ORF3 ORF3 NNP cord-013178-li1x1m25 386 40 in in IN cord-013178-li1x1m25 386 41 porcine porcine JJ cord-013178-li1x1m25 386 42 PBMCs pbmc NNS cord-013178-li1x1m25 386 43 and and CC cord-013178-li1x1m25 386 44 detecting detect VBG cord-013178-li1x1m25 386 45 apoptosis apoptosis NN cord-013178-li1x1m25 386 46 with with IN cord-013178-li1x1m25 386 47 a a DT cord-013178-li1x1m25 386 48 TUNEL TUNEL NNP cord-013178-li1x1m25 386 49 assay assay NN cord-013178-li1x1m25 386 50 [ [ -LRB- cord-013178-li1x1m25 386 51 50 50 CD cord-013178-li1x1m25 386 52 ] ] -RRB- cord-013178-li1x1m25 386 53 . . . cord-013178-li1x1m25 387 1 Therefore therefore RB cord-013178-li1x1m25 387 2 , , , cord-013178-li1x1m25 387 3 the the DT cord-013178-li1x1m25 387 4 author author NN cord-013178-li1x1m25 387 5 explored explore VBD cord-013178-li1x1m25 387 6 the the DT cord-013178-li1x1m25 387 7 relation relation NN cord-013178-li1x1m25 387 8 of of IN cord-013178-li1x1m25 387 9 the the DT cord-013178-li1x1m25 387 10 capsid capsid NN cord-013178-li1x1m25 387 11 , , , cord-013178-li1x1m25 387 12 p53 p53 NN cord-013178-li1x1m25 387 13 protein protein NN cord-013178-li1x1m25 387 14 , , , cord-013178-li1x1m25 387 15 and and CC cord-013178-li1x1m25 387 16 the the DT cord-013178-li1x1m25 387 17 ORF3 ORF3 NNP cord-013178-li1x1m25 387 18 protein protein NN cord-013178-li1x1m25 387 19 by by IN cord-013178-li1x1m25 387 20 IFA IFA NNP cord-013178-li1x1m25 387 21 and and CC cord-013178-li1x1m25 387 22 shed shed VBD cord-013178-li1x1m25 387 23 light light NN cord-013178-li1x1m25 387 24 on on IN cord-013178-li1x1m25 387 25 p53 p53 NN cord-013178-li1x1m25 387 26 protein protein NN cord-013178-li1x1m25 387 27 ( ( -LRB- cord-013178-li1x1m25 387 28 the the DT cord-013178-li1x1m25 387 29 marker marker NN cord-013178-li1x1m25 387 30 of of IN cord-013178-li1x1m25 387 31 apoptosis apoptosis NN cord-013178-li1x1m25 387 32 ) ) -RRB- cord-013178-li1x1m25 387 33 accompanied accompany VBD cord-013178-li1x1m25 387 34 the the DT cord-013178-li1x1m25 387 35 peptide peptide NN cord-013178-li1x1m25 387 36 ( ( -LRB- cord-013178-li1x1m25 387 37 residues residue NNS cord-013178-li1x1m25 387 38 35 35 CD cord-013178-li1x1m25 387 39 - - SYM cord-013178-li1x1m25 387 40 66 66 CD cord-013178-li1x1m25 387 41 , , , cord-013178-li1x1m25 387 42 P126 p126 CD cord-013178-li1x1m25 387 43 ) ) -RRB- cord-013178-li1x1m25 387 44 of of IN cord-013178-li1x1m25 387 45 the the DT cord-013178-li1x1m25 387 46 ORF3 ORF3 NNP cord-013178-li1x1m25 387 47 protein protein NN cord-013178-li1x1m25 387 48 in in IN cord-013178-li1x1m25 387 49 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 387 50 PBMCs pbmc NNS cord-013178-li1x1m25 387 51 . . . cord-013178-li1x1m25 388 1 It -PRON- PRP cord-013178-li1x1m25 388 2 is be VBZ cord-013178-li1x1m25 388 3 worth worth JJ cord-013178-li1x1m25 388 4 highlighting highlight VBG cord-013178-li1x1m25 388 5 that that IN cord-013178-li1x1m25 388 6 3 3 CD cord-013178-li1x1m25 388 7 - - SYM cord-013178-li1x1m25 388 8 5 5 CD cord-013178-li1x1m25 388 9 % % NN cord-013178-li1x1m25 388 10 of of IN cord-013178-li1x1m25 388 11 PBMCs pbmc NNS cord-013178-li1x1m25 388 12 were be VBD cord-013178-li1x1m25 388 13 positive positive JJ cord-013178-li1x1m25 388 14 for for IN cord-013178-li1x1m25 388 15 some some DT cord-013178-li1x1m25 388 16 antibodies antibody NNS cord-013178-li1x1m25 388 17 staining stain VBG cord-013178-li1x1m25 388 18 , , , cord-013178-li1x1m25 388 19 including include VBG cord-013178-li1x1m25 388 20 anti anti JJ cord-013178-li1x1m25 388 21 - - JJ cord-013178-li1x1m25 388 22 ORF3 ORF3 NNP cord-013178-li1x1m25 388 23 mAb mAb . cord-013178-li1x1m25 388 24 ( ( -LRB- cord-013178-li1x1m25 388 25 7D3 7d3 CD cord-013178-li1x1m25 388 26 ) ) -RRB- cord-013178-li1x1m25 388 27 staining staining NN cord-013178-li1x1m25 388 28 , , , cord-013178-li1x1m25 388 29 anti anti JJ cord-013178-li1x1m25 388 30 - - JJ cord-013178-li1x1m25 388 31 p53 p53 JJ cord-013178-li1x1m25 388 32 protein protein NN cord-013178-li1x1m25 388 33 rabbit rabbit NN cord-013178-li1x1m25 388 34 polyclonal polyclonal JJ cord-013178-li1x1m25 388 35 antibody antibody NN cord-013178-li1x1m25 388 36 staining staining NN cord-013178-li1x1m25 388 37 , , , cord-013178-li1x1m25 388 38 and and CC cord-013178-li1x1m25 388 39 TUNEL TUNEL NNP cord-013178-li1x1m25 388 40 assay assay NN cord-013178-li1x1m25 388 41 ( ( -LRB- cord-013178-li1x1m25 388 42 Figure Figure NNP cord-013178-li1x1m25 388 43 S3 S3 NNP cord-013178-li1x1m25 388 44 ) ) -RRB- cord-013178-li1x1m25 388 45 . . . cord-013178-li1x1m25 389 1 There there EX cord-013178-li1x1m25 389 2 is be VBZ cord-013178-li1x1m25 389 3 a a DT cord-013178-li1x1m25 389 4 strong strong JJ cord-013178-li1x1m25 389 5 probability probability NN cord-013178-li1x1m25 389 6 that that IN cord-013178-li1x1m25 389 7 3 3 CD cord-013178-li1x1m25 389 8 - - SYM cord-013178-li1x1m25 389 9 5 5 CD cord-013178-li1x1m25 389 10 % % NN cord-013178-li1x1m25 389 11 of of IN cord-013178-li1x1m25 389 12 PBMCs pbmc NNS cord-013178-li1x1m25 389 13 were be VBD cord-013178-li1x1m25 389 14 undergoing undergo VBG cord-013178-li1x1m25 389 15 the the DT cord-013178-li1x1m25 389 16 process process NN cord-013178-li1x1m25 389 17 of of IN cord-013178-li1x1m25 389 18 apoptosis apoptosis NN cord-013178-li1x1m25 389 19 . . . cord-013178-li1x1m25 390 1 However however RB cord-013178-li1x1m25 390 2 , , , cord-013178-li1x1m25 390 3 the the DT cord-013178-li1x1m25 390 4 data datum NNS cord-013178-li1x1m25 390 5 revealed reveal VBD cord-013178-li1x1m25 390 6 that that IN cord-013178-li1x1m25 390 7 the the DT cord-013178-li1x1m25 390 8 variable variable JJ cord-013178-li1x1m25 390 9 percentage percentage NN cord-013178-li1x1m25 390 10 ( ( -LRB- cord-013178-li1x1m25 390 11 4 4 CD cord-013178-li1x1m25 390 12 - - SYM cord-013178-li1x1m25 390 13 82 82 CD cord-013178-li1x1m25 390 14 % % NN cord-013178-li1x1m25 390 15 ) ) -RRB- cord-013178-li1x1m25 390 16 of of IN cord-013178-li1x1m25 390 17 PBMCs pbmc NNS cord-013178-li1x1m25 390 18 was be VBD cord-013178-li1x1m25 390 19 positive positive JJ cord-013178-li1x1m25 390 20 for for IN cord-013178-li1x1m25 390 21 anti anti JJ cord-013178-li1x1m25 390 22 - - JJ cord-013178-li1x1m25 390 23 capsid capsid JJ cord-013178-li1x1m25 390 24 mAbs mAbs NNP cord-013178-li1x1m25 390 25 ( ( -LRB- cord-013178-li1x1m25 390 26 1H3 1h3 CD cord-013178-li1x1m25 390 27 , , , cord-013178-li1x1m25 390 28 6B8 6b8 CD cord-013178-li1x1m25 390 29 , , , cord-013178-li1x1m25 390 30 and and CC cord-013178-li1x1m25 390 31 36A9 36a9 LS cord-013178-li1x1m25 390 32 ) ) -RRB- cord-013178-li1x1m25 390 33 staining staining NN cord-013178-li1x1m25 390 34 and and CC cord-013178-li1x1m25 390 35 anti anti JJ cord-013178-li1x1m25 390 36 - - JJ cord-013178-li1x1m25 390 37 VLP vlp JJ cord-013178-li1x1m25 390 38 rabbit rabbit NN cord-013178-li1x1m25 390 39 serum serum NN cord-013178-li1x1m25 390 40 staining staining NN cord-013178-li1x1m25 390 41 . . . cord-013178-li1x1m25 391 1 It -PRON- PRP cord-013178-li1x1m25 391 2 was be VBD cord-013178-li1x1m25 391 3 because because IN cord-013178-li1x1m25 391 4 these these DT cord-013178-li1x1m25 391 5 mAbs mAbs NNP cord-013178-li1x1m25 391 6 or or CC cord-013178-li1x1m25 391 7 antiserum antiserum NN cord-013178-li1x1m25 391 8 recognized recognize VBD cord-013178-li1x1m25 391 9 different different JJ cord-013178-li1x1m25 391 10 epitopes epitope NNS cord-013178-li1x1m25 391 11 of of IN cord-013178-li1x1m25 391 12 proteins protein NNS cord-013178-li1x1m25 391 13 presented present VBN cord-013178-li1x1m25 391 14 in in IN cord-013178-li1x1m25 391 15 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 391 16 cells cell NNS cord-013178-li1x1m25 391 17 . . . cord-013178-li1x1m25 392 1 That that DT cord-013178-li1x1m25 392 2 means mean VBZ cord-013178-li1x1m25 392 3 that that IN cord-013178-li1x1m25 392 4 the the DT cord-013178-li1x1m25 392 5 variety variety NN cord-013178-li1x1m25 392 6 of of IN cord-013178-li1x1m25 392 7 interaction interaction NN cord-013178-li1x1m25 392 8 between between IN cord-013178-li1x1m25 392 9 antigen antigen NN cord-013178-li1x1m25 392 10 - - HYPH cord-013178-li1x1m25 392 11 binding bind VBG cord-013178-li1x1m25 392 12 sites site NNS cord-013178-li1x1m25 392 13 of of IN cord-013178-li1x1m25 392 14 antibodies antibody NNS cord-013178-li1x1m25 392 15 and and CC cord-013178-li1x1m25 392 16 epitopes epitope NNS cord-013178-li1x1m25 392 17 , , , cord-013178-li1x1m25 392 18 which which WDT cord-013178-li1x1m25 392 19 includes include VBZ cord-013178-li1x1m25 392 20 linear linear JJ cord-013178-li1x1m25 392 21 form form NN cord-013178-li1x1m25 392 22 epitopes epitope NNS cord-013178-li1x1m25 392 23 , , , cord-013178-li1x1m25 392 24 conformational conformational JJ cord-013178-li1x1m25 392 25 epitopes epitope NNS cord-013178-li1x1m25 392 26 , , , cord-013178-li1x1m25 392 27 degradation degradation NN cord-013178-li1x1m25 392 28 , , , cord-013178-li1x1m25 392 29 and and CC cord-013178-li1x1m25 392 30 different different JJ cord-013178-li1x1m25 392 31 PCV2 pcv2 JJ cord-013178-li1x1m25 392 32 strains strain NNS cord-013178-li1x1m25 392 33 . . . cord-013178-li1x1m25 393 1 Curiously curiously RB cord-013178-li1x1m25 393 2 , , , cord-013178-li1x1m25 393 3 this this DT cord-013178-li1x1m25 393 4 study study NN cord-013178-li1x1m25 393 5 ( ( -LRB- cord-013178-li1x1m25 393 6 Figure Figure NNP cord-013178-li1x1m25 393 7 S3 S3 NNP cord-013178-li1x1m25 393 8 ) ) -RRB- cord-013178-li1x1m25 393 9 showed show VBD cord-013178-li1x1m25 393 10 that that IN cord-013178-li1x1m25 393 11 the the DT cord-013178-li1x1m25 393 12 percentage percentage NN cord-013178-li1x1m25 393 13 of of IN cord-013178-li1x1m25 393 14 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 393 15 PBMCs pbmc NNS cord-013178-li1x1m25 393 16 ( ( -LRB- cord-013178-li1x1m25 393 17 by by IN cord-013178-li1x1m25 393 18 mAb mab NN cord-013178-li1x1m25 393 19 1H3 1h3 CD cord-013178-li1x1m25 393 20 staining staining NN cord-013178-li1x1m25 393 21 ) ) -RRB- cord-013178-li1x1m25 393 22 was be VBD cord-013178-li1x1m25 393 23 about about RB cord-013178-li1x1m25 393 24 20-fold 20-fold CD cord-013178-li1x1m25 394 1 the the DT cord-013178-li1x1m25 394 2 rate rate NN cord-013178-li1x1m25 394 3 of of IN cord-013178-li1x1m25 394 4 ORF3-positive ORF3-positive NNP cord-013178-li1x1m25 394 5 PBMCs pbmc NNS cord-013178-li1x1m25 394 6 ( ( -LRB- cord-013178-li1x1m25 394 7 by by IN cord-013178-li1x1m25 394 8 mAb mab NN cord-013178-li1x1m25 394 9 7D3 7d3 CD cord-013178-li1x1m25 394 10 staining staining NN cord-013178-li1x1m25 394 11 ) ) -RRB- cord-013178-li1x1m25 394 12 or or CC cord-013178-li1x1m25 394 13 the the DT cord-013178-li1x1m25 394 14 percentage percentage NN cord-013178-li1x1m25 394 15 of of IN cord-013178-li1x1m25 394 16 p53-positive p53-positive JJ cord-013178-li1x1m25 394 17 PBMCs pbmc NNS cord-013178-li1x1m25 394 18 . . . cord-013178-li1x1m25 395 1 It -PRON- PRP cord-013178-li1x1m25 395 2 seems seem VBZ cord-013178-li1x1m25 395 3 likely likely JJ cord-013178-li1x1m25 395 4 that that IN cord-013178-li1x1m25 395 5 not not RB cord-013178-li1x1m25 395 6 all all DT cord-013178-li1x1m25 395 7 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 395 8 cells cell NNS cord-013178-li1x1m25 395 9 contained contain VBD cord-013178-li1x1m25 395 10 ORF3 ORF3 NNP cord-013178-li1x1m25 395 11 proteins protein NNS cord-013178-li1x1m25 395 12 or or CC cord-013178-li1x1m25 395 13 p53 p53 NN cord-013178-li1x1m25 395 14 protein protein NN cord-013178-li1x1m25 395 15 . . . cord-013178-li1x1m25 396 1 This this DT cord-013178-li1x1m25 396 2 finding finding NN cord-013178-li1x1m25 396 3 concurs concur VBZ cord-013178-li1x1m25 396 4 with with IN cord-013178-li1x1m25 396 5 the the DT cord-013178-li1x1m25 396 6 previous previous JJ cord-013178-li1x1m25 396 7 study study NN cord-013178-li1x1m25 396 8 , , , cord-013178-li1x1m25 396 9 which which WDT cord-013178-li1x1m25 396 10 indicated indicate VBD cord-013178-li1x1m25 396 11 that that IN cord-013178-li1x1m25 396 12 the the DT cord-013178-li1x1m25 396 13 apoptosis apoptosis NN cord-013178-li1x1m25 396 14 statistically statistically RB cord-013178-li1x1m25 396 15 decreases decrease VBZ cord-013178-li1x1m25 396 16 in in IN cord-013178-li1x1m25 396 17 the the DT cord-013178-li1x1m25 396 18 initial initial JJ cord-013178-li1x1m25 396 19 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 396 20 pigs pig NNS cord-013178-li1x1m25 396 21 [ [ -LRB- cord-013178-li1x1m25 396 22 60 60 CD cord-013178-li1x1m25 396 23 ] ] -RRB- cord-013178-li1x1m25 396 24 . . . cord-013178-li1x1m25 397 1 On on IN cord-013178-li1x1m25 397 2 the the DT cord-013178-li1x1m25 397 3 other other JJ cord-013178-li1x1m25 397 4 hand hand NN cord-013178-li1x1m25 397 5 , , , cord-013178-li1x1m25 397 6 this this DT cord-013178-li1x1m25 397 7 study study NN cord-013178-li1x1m25 397 8 showed show VBD cord-013178-li1x1m25 397 9 the the DT cord-013178-li1x1m25 397 10 ORF3 ORF3 NNP cord-013178-li1x1m25 397 11 protein protein NN cord-013178-li1x1m25 397 12 colocalized colocalize VBN cord-013178-li1x1m25 397 13 with with IN cord-013178-li1x1m25 397 14 the the DT cord-013178-li1x1m25 397 15 capsid capsid JJ cord-013178-li1x1m25 397 16 protein protein NN cord-013178-li1x1m25 397 17 marker marker NN cord-013178-li1x1m25 397 18 of of IN cord-013178-li1x1m25 397 19 PCV2 PCV2 NNP cord-013178-li1x1m25 397 20 . . . cord-013178-li1x1m25 398 1 Moreover moreover RB cord-013178-li1x1m25 398 2 , , , cord-013178-li1x1m25 398 3 the the DT cord-013178-li1x1m25 398 4 p53 p53 NN cord-013178-li1x1m25 398 5 protein protein NN cord-013178-li1x1m25 398 6 was be VBD cord-013178-li1x1m25 398 7 also also RB cord-013178-li1x1m25 398 8 colocalized colocalize VBN cord-013178-li1x1m25 398 9 with with IN cord-013178-li1x1m25 398 10 the the DT cord-013178-li1x1m25 398 11 ORF3 ORF3 NNP cord-013178-li1x1m25 398 12 protein protein NN cord-013178-li1x1m25 398 13 . . . cord-013178-li1x1m25 399 1 Remarkably remarkably RB cord-013178-li1x1m25 399 2 , , , cord-013178-li1x1m25 399 3 the the DT cord-013178-li1x1m25 399 4 p53 p53 NN cord-013178-li1x1m25 399 5 / / SYM cord-013178-li1x1m25 399 6 ORF3 ORF3 NNP cord-013178-li1x1m25 399 7 dual dual JJ cord-013178-li1x1m25 399 8 - - HYPH cord-013178-li1x1m25 399 9 positive positive JJ cord-013178-li1x1m25 399 10 cell cell NN cord-013178-li1x1m25 399 11 presented present VBD cord-013178-li1x1m25 399 12 a a DT cord-013178-li1x1m25 399 13 segmented segmented JJ cord-013178-li1x1m25 399 14 nucleus nucleus NN cord-013178-li1x1m25 399 15 ( ( -LRB- cord-013178-li1x1m25 399 16 Figure figure NN cord-013178-li1x1m25 399 17 3W 3w CD cord-013178-li1x1m25 399 18 ) ) -RRB- cord-013178-li1x1m25 399 19 . . . cord-013178-li1x1m25 400 1 However however RB cord-013178-li1x1m25 400 2 , , , cord-013178-li1x1m25 400 3 these these DT cord-013178-li1x1m25 400 4 p53 p53 NN cord-013178-li1x1m25 400 5 / / SYM cord-013178-li1x1m25 400 6 ORF3 ORF3 NNP cord-013178-li1x1m25 400 7 dual dual JJ cord-013178-li1x1m25 400 8 - - HYPH cord-013178-li1x1m25 400 9 positive positive JJ cord-013178-li1x1m25 400 10 cells cell NNS cord-013178-li1x1m25 400 11 were be VBD cord-013178-li1x1m25 400 12 very very RB cord-013178-li1x1m25 400 13 few few JJ cord-013178-li1x1m25 400 14 in in IN cord-013178-li1x1m25 400 15 these these DT cord-013178-li1x1m25 400 16 samples sample NNS cord-013178-li1x1m25 400 17 . . . cord-013178-li1x1m25 401 1 This this DT cord-013178-li1x1m25 401 2 finding finding NN cord-013178-li1x1m25 401 3 will will MD cord-013178-li1x1m25 401 4 be be VB cord-013178-li1x1m25 401 5 confirmed confirm VBN cord-013178-li1x1m25 401 6 by by IN cord-013178-li1x1m25 401 7 flow flow NN cord-013178-li1x1m25 401 8 cytometry cytometry NN cord-013178-li1x1m25 401 9 in in IN cord-013178-li1x1m25 401 10 the the DT cord-013178-li1x1m25 401 11 future future NN cord-013178-li1x1m25 401 12 . . . cord-013178-li1x1m25 402 1 Although although IN cord-013178-li1x1m25 402 2 a a DT cord-013178-li1x1m25 402 3 previous previous JJ cord-013178-li1x1m25 402 4 study study NN cord-013178-li1x1m25 402 5 indicated indicate VBD cord-013178-li1x1m25 402 6 that that IN cord-013178-li1x1m25 402 7 PCV2-infected PCV2-infected NNP cord-013178-li1x1m25 402 8 pigs pig NNS cord-013178-li1x1m25 402 9 had have VBD cord-013178-li1x1m25 402 10 a a DT cord-013178-li1x1m25 402 11 reduction reduction NN cord-013178-li1x1m25 402 12 of of IN cord-013178-li1x1m25 402 13 lymphocytes lymphocyte NNS cord-013178-li1x1m25 402 14 in in IN cord-013178-li1x1m25 402 15 the the DT cord-013178-li1x1m25 402 16 peripheral peripheral JJ cord-013178-li1x1m25 402 17 blood blood NN cord-013178-li1x1m25 402 18 [ [ -LRB- cord-013178-li1x1m25 402 19 11 11 CD cord-013178-li1x1m25 402 20 ] ] -RRB- cord-013178-li1x1m25 402 21 , , , cord-013178-li1x1m25 402 22 the the DT cord-013178-li1x1m25 402 23 mechanism mechanism NN cord-013178-li1x1m25 402 24 of of IN cord-013178-li1x1m25 402 25 lymphocyte lymphocyte NN cord-013178-li1x1m25 402 26 lysis lysis NN cord-013178-li1x1m25 402 27 was be VBD cord-013178-li1x1m25 402 28 still still RB cord-013178-li1x1m25 402 29 unclear unclear JJ cord-013178-li1x1m25 402 30 . . . cord-013178-li1x1m25 403 1 This this DT cord-013178-li1x1m25 403 2 study study NN cord-013178-li1x1m25 403 3 confirmed confirm VBD cord-013178-li1x1m25 403 4 that that IN cord-013178-li1x1m25 403 5 the the DT cord-013178-li1x1m25 403 6 ORF3 ORF3 NNP cord-013178-li1x1m25 403 7 protein protein NN cord-013178-li1x1m25 403 8 was be VBD cord-013178-li1x1m25 403 9 related relate VBN cord-013178-li1x1m25 403 10 to to IN cord-013178-li1x1m25 403 11 the the DT cord-013178-li1x1m25 403 12 p53 p53 NN cord-013178-li1x1m25 403 13 protein protein NN cord-013178-li1x1m25 403 14 ( ( -LRB- cord-013178-li1x1m25 403 15 apoptosis apoptosis NN cord-013178-li1x1m25 403 16 marker marker NN cord-013178-li1x1m25 403 17 ) ) -RRB- cord-013178-li1x1m25 403 18 in in IN cord-013178-li1x1m25 403 19 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 403 20 PBMCs pbmc NNS cord-013178-li1x1m25 403 21 , , , cord-013178-li1x1m25 403 22 while while IN cord-013178-li1x1m25 403 23 other other JJ cord-013178-li1x1m25 403 24 factors factor NNS cord-013178-li1x1m25 403 25 ( ( -LRB- cord-013178-li1x1m25 403 26 proliferative proliferative JJ cord-013178-li1x1m25 403 27 activity activity NN cord-013178-li1x1m25 403 28 [ [ -LRB- cord-013178-li1x1m25 403 29 60 60 CD cord-013178-li1x1m25 403 30 ] ] -RRB- cord-013178-li1x1m25 403 31 , , , cord-013178-li1x1m25 403 32 caspases caspase VBZ cord-013178-li1x1m25 403 33 8 8 CD cord-013178-li1x1m25 403 34 and and CC cord-013178-li1x1m25 403 35 3 3 CD cord-013178-li1x1m25 403 36 [ [ -LRB- cord-013178-li1x1m25 403 37 34 34 CD cord-013178-li1x1m25 403 38 , , , cord-013178-li1x1m25 403 39 61 61 CD cord-013178-li1x1m25 403 40 ] ] -RRB- cord-013178-li1x1m25 403 41 , , , cord-013178-li1x1m25 403 42 granzymes granzymes NNPS cord-013178-li1x1m25 403 43 [ [ -LRB- cord-013178-li1x1m25 403 44 62 62 CD cord-013178-li1x1m25 403 45 ] ] -RRB- cord-013178-li1x1m25 403 46 , , , cord-013178-li1x1m25 403 47 or or CC cord-013178-li1x1m25 403 48 corticosteroids corticosteroid NNS cord-013178-li1x1m25 403 49 [ [ -LRB- cord-013178-li1x1m25 403 50 63 63 CD cord-013178-li1x1m25 403 51 , , , cord-013178-li1x1m25 403 52 64 64 CD cord-013178-li1x1m25 403 53 ] ] -RRB- cord-013178-li1x1m25 403 54 ) ) -RRB- cord-013178-li1x1m25 403 55 causing cause VBG cord-013178-li1x1m25 403 56 lymphocyte lymphocyte NN cord-013178-li1x1m25 403 57 lysis lysis NN cord-013178-li1x1m25 403 58 or or CC cord-013178-li1x1m25 403 59 depletion depletion NN cord-013178-li1x1m25 403 60 need need NN cord-013178-li1x1m25 403 61 to to TO cord-013178-li1x1m25 403 62 be be VB cord-013178-li1x1m25 403 63 considered consider VBN cord-013178-li1x1m25 403 64 . . . cord-013178-li1x1m25 404 1 Although although IN cord-013178-li1x1m25 404 2 the the DT cord-013178-li1x1m25 404 3 experiment experiment NN cord-013178-li1x1m25 404 4 on on IN cord-013178-li1x1m25 404 5 apoptosis apoptosis NN cord-013178-li1x1m25 404 6 in in IN cord-013178-li1x1m25 404 7 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 404 8 PBMCs PBMCs NNP cord-013178-li1x1m25 404 9 was be VBD cord-013178-li1x1m25 404 10 carried carry VBN cord-013178-li1x1m25 404 11 out out RP cord-013178-li1x1m25 404 12 by by IN cord-013178-li1x1m25 404 13 the the DT cord-013178-li1x1m25 404 14 TUNEL TUNEL NNP cord-013178-li1x1m25 404 15 assay assay NN cord-013178-li1x1m25 404 16 ( ( -LRB- cord-013178-li1x1m25 404 17 Figure figure NN cord-013178-li1x1m25 404 18 4 4 CD cord-013178-li1x1m25 404 19 ) ) -RRB- cord-013178-li1x1m25 404 20 , , , cord-013178-li1x1m25 404 21 it -PRON- PRP cord-013178-li1x1m25 404 22 did do VBD cord-013178-li1x1m25 404 23 not not RB cord-013178-li1x1m25 404 24 clarify clarify VB cord-013178-li1x1m25 404 25 the the DT cord-013178-li1x1m25 404 26 relationship relationship NN cord-013178-li1x1m25 404 27 between between IN cord-013178-li1x1m25 404 28 the the DT cord-013178-li1x1m25 404 29 TUNEL TUNEL NNP cord-013178-li1x1m25 404 30 result result NN cord-013178-li1x1m25 404 31 and and CC cord-013178-li1x1m25 404 32 the the DT cord-013178-li1x1m25 404 33 ORF3 ORF3 NNP cord-013178-li1x1m25 404 34 protein protein NN cord-013178-li1x1m25 404 35 in in IN cord-013178-li1x1m25 404 36 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 404 37 cells cell NNS cord-013178-li1x1m25 404 38 . . . cord-013178-li1x1m25 405 1 Only only RB cord-013178-li1x1m25 405 2 the the DT cord-013178-li1x1m25 405 3 IFA IFA NNP cord-013178-li1x1m25 405 4 data datum NNS cord-013178-li1x1m25 405 5 confirm confirm VBP cord-013178-li1x1m25 405 6 previous previous JJ cord-013178-li1x1m25 405 7 reports report NNS cord-013178-li1x1m25 405 8 that that IN cord-013178-li1x1m25 405 9 exogenous exogenous JJ cord-013178-li1x1m25 405 10 ORF3 ORF3 NNP cord-013178-li1x1m25 405 11 protein protein NN cord-013178-li1x1m25 405 12 was be VBD cord-013178-li1x1m25 405 13 related relate VBN cord-013178-li1x1m25 405 14 to to IN cord-013178-li1x1m25 405 15 the the DT cord-013178-li1x1m25 405 16 accumulation accumulation NN cord-013178-li1x1m25 405 17 of of IN cord-013178-li1x1m25 405 18 p53 p53 NN cord-013178-li1x1m25 405 19 protein protein NN cord-013178-li1x1m25 405 20 [ [ -LRB- cord-013178-li1x1m25 405 21 37 37 CD cord-013178-li1x1m25 405 22 , , , cord-013178-li1x1m25 405 23 42 42 CD cord-013178-li1x1m25 405 24 ] ] -RRB- cord-013178-li1x1m25 405 25 , , , cord-013178-li1x1m25 405 26 but but CC cord-013178-li1x1m25 405 27 more more JJR cord-013178-li1x1m25 405 28 proof proof NN cord-013178-li1x1m25 405 29 are be VBP cord-013178-li1x1m25 405 30 needed need VBN cord-013178-li1x1m25 405 31 to to TO cord-013178-li1x1m25 405 32 make make VB cord-013178-li1x1m25 405 33 sure sure JJ cord-013178-li1x1m25 405 34 the the DT cord-013178-li1x1m25 405 35 ORF3 ORF3 NNP cord-013178-li1x1m25 405 36 protein protein NN cord-013178-li1x1m25 405 37 is be VBZ cord-013178-li1x1m25 405 38 a a DT cord-013178-li1x1m25 405 39 major major JJ cord-013178-li1x1m25 405 40 factor factor NN cord-013178-li1x1m25 405 41 leading lead VBG cord-013178-li1x1m25 405 42 to to IN cord-013178-li1x1m25 405 43 apoptosis apoptosis NN cord-013178-li1x1m25 405 44 in in IN cord-013178-li1x1m25 405 45 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 405 46 cells cell NNS cord-013178-li1x1m25 405 47 [ [ -LRB- cord-013178-li1x1m25 405 48 34 34 CD cord-013178-li1x1m25 405 49 , , , cord-013178-li1x1m25 405 50 37 37 CD cord-013178-li1x1m25 405 51 , , , cord-013178-li1x1m25 405 52 42 42 CD cord-013178-li1x1m25 405 53 ] ] -RRB- cord-013178-li1x1m25 405 54 and and CC cord-013178-li1x1m25 405 55 depletion depletion NN cord-013178-li1x1m25 405 56 of of IN cord-013178-li1x1m25 405 57 lymphocytes lymphocyte NNS cord-013178-li1x1m25 405 58 . . . cord-013178-li1x1m25 406 1 PCV2 PCV2 NNP cord-013178-li1x1m25 406 2 is be VBZ cord-013178-li1x1m25 406 3 one one CD cord-013178-li1x1m25 406 4 of of IN cord-013178-li1x1m25 406 5 the the DT cord-013178-li1x1m25 406 6 most most RBS cord-013178-li1x1m25 406 7 critical critical JJ cord-013178-li1x1m25 406 8 pathogens pathogen NNS cord-013178-li1x1m25 406 9 in in IN cord-013178-li1x1m25 406 10 modern modern JJ cord-013178-li1x1m25 406 11 swine swine NN cord-013178-li1x1m25 406 12 production production NN cord-013178-li1x1m25 406 13 and and CC cord-013178-li1x1m25 406 14 causes cause VBZ cord-013178-li1x1m25 406 15 endemic endemic JJ cord-013178-li1x1m25 406 16 disease disease NN cord-013178-li1x1m25 406 17 in in IN cord-013178-li1x1m25 406 18 pig pig NN cord-013178-li1x1m25 406 19 farms farm NNS cord-013178-li1x1m25 406 20 [ [ -LRB- cord-013178-li1x1m25 406 21 7 7 CD cord-013178-li1x1m25 406 22 ] ] -RRB- cord-013178-li1x1m25 406 23 . . . cord-013178-li1x1m25 407 1 The the DT cord-013178-li1x1m25 407 2 commercial commercial JJ cord-013178-li1x1m25 407 3 vaccines vaccine NNS cord-013178-li1x1m25 407 4 were be VBD cord-013178-li1x1m25 407 5 confirmed confirm VBN cord-013178-li1x1m25 407 6 protective protective JJ cord-013178-li1x1m25 407 7 in in IN cord-013178-li1x1m25 407 8 the the DT cord-013178-li1x1m25 407 9 field field NN cord-013178-li1x1m25 407 10 against against IN cord-013178-li1x1m25 407 11 PCV2 PCV2 NNP cord-013178-li1x1m25 407 12 and and CC cord-013178-li1x1m25 407 13 mainly mainly RB cord-013178-li1x1m25 407 14 administered administer VBN cord-013178-li1x1m25 407 15 to to IN cord-013178-li1x1m25 407 16 suckers sucker NNS cord-013178-li1x1m25 407 17 in in IN cord-013178-li1x1m25 407 18 herds herd NNS cord-013178-li1x1m25 407 19 with with IN cord-013178-li1x1m25 407 20 PCV2 pcv2 JJ cord-013178-li1x1m25 407 21 infection infection NN cord-013178-li1x1m25 407 22 [ [ -LRB- cord-013178-li1x1m25 407 23 13 13 CD cord-013178-li1x1m25 407 24 , , , cord-013178-li1x1m25 407 25 14 14 CD cord-013178-li1x1m25 407 26 , , , cord-013178-li1x1m25 407 27 [ [ -LRB- cord-013178-li1x1m25 407 28 65 65 CD cord-013178-li1x1m25 407 29 ] ] -RRB- cord-013178-li1x1m25 407 30 [ [ -LRB- cord-013178-li1x1m25 407 31 66 66 CD cord-013178-li1x1m25 407 32 ] ] -RRB- cord-013178-li1x1m25 408 1 [ [ -LRB- cord-013178-li1x1m25 408 2 67 67 CD cord-013178-li1x1m25 408 3 ] ] -RRB- cord-013178-li1x1m25 408 4 . . . cord-013178-li1x1m25 409 1 Most Most JJS cord-013178-li1x1m25 409 2 researchers researcher NNS cord-013178-li1x1m25 409 3 utilized utilize VBD cord-013178-li1x1m25 409 4 the the DT cord-013178-li1x1m25 409 5 quantitative quantitative JJ cord-013178-li1x1m25 409 6 real real JJ cord-013178-li1x1m25 409 7 - - HYPH cord-013178-li1x1m25 409 8 time time NN cord-013178-li1x1m25 409 9 PCR PCR NNP cord-013178-li1x1m25 409 10 to to TO cord-013178-li1x1m25 409 11 detect detect VB cord-013178-li1x1m25 409 12 PCV2 pcv2 JJ cord-013178-li1x1m25 409 13 nucleic nucleic JJ cord-013178-li1x1m25 409 14 acid acid NN cord-013178-li1x1m25 409 15 , , , cord-013178-li1x1m25 409 16 and and CC cord-013178-li1x1m25 409 17 then then RB cord-013178-li1x1m25 409 18 they -PRON- PRP cord-013178-li1x1m25 409 19 evaluated evaluate VBD cord-013178-li1x1m25 409 20 the the DT cord-013178-li1x1m25 409 21 efficacy efficacy NN cord-013178-li1x1m25 409 22 of of IN cord-013178-li1x1m25 409 23 vaccination vaccination NN cord-013178-li1x1m25 409 24 in in IN cord-013178-li1x1m25 409 25 regard regard NN cord-013178-li1x1m25 409 26 to to IN cord-013178-li1x1m25 409 27 PCV2 pcv2 JJ cord-013178-li1x1m25 409 28 viremia viremia NN cord-013178-li1x1m25 409 29 in in IN cord-013178-li1x1m25 409 30 pigs pig NNS cord-013178-li1x1m25 409 31 [ [ -LRB- cord-013178-li1x1m25 409 32 13 13 CD cord-013178-li1x1m25 409 33 , , , cord-013178-li1x1m25 409 34 14 14 CD cord-013178-li1x1m25 409 35 , , , cord-013178-li1x1m25 409 36 66 66 CD cord-013178-li1x1m25 409 37 ] ] -RRB- cord-013178-li1x1m25 409 38 . . . cord-013178-li1x1m25 410 1 However however RB cord-013178-li1x1m25 410 2 , , , cord-013178-li1x1m25 410 3 little little JJ cord-013178-li1x1m25 410 4 is be VBZ cord-013178-li1x1m25 410 5 known know VBN cord-013178-li1x1m25 410 6 about about IN cord-013178-li1x1m25 410 7 the the DT cord-013178-li1x1m25 410 8 capsid capsid JJ cord-013178-li1x1m25 410 9 protein protein NN cord-013178-li1x1m25 410 10 or or CC cord-013178-li1x1m25 410 11 the the DT cord-013178-li1x1m25 410 12 ORF3 ORF3 NNP cord-013178-li1x1m25 410 13 protein protein NN cord-013178-li1x1m25 410 14 in in IN cord-013178-li1x1m25 410 15 blood blood NN cord-013178-li1x1m25 410 16 from from IN cord-013178-li1x1m25 410 17 the the DT cord-013178-li1x1m25 410 18 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 410 19 herd herd NN cord-013178-li1x1m25 410 20 with with IN cord-013178-li1x1m25 410 21 vaccination vaccination NN cord-013178-li1x1m25 410 22 . . . cord-013178-li1x1m25 411 1 Although although IN cord-013178-li1x1m25 411 2 the the DT cord-013178-li1x1m25 411 3 capsid capsid JJ cord-013178-li1x1m25 411 4 antigen antigen NN cord-013178-li1x1m25 411 5 - - HYPH cord-013178-li1x1m25 411 6 ELISA ELISA NNP cord-013178-li1x1m25 411 7 could could MD cord-013178-li1x1m25 411 8 detect detect VB cord-013178-li1x1m25 411 9 capsid capsid NN cord-013178-li1x1m25 411 10 protein protein NN cord-013178-li1x1m25 411 11 , , , cord-013178-li1x1m25 411 12 this this DT cord-013178-li1x1m25 411 13 assay assay NN cord-013178-li1x1m25 411 14 could could MD cord-013178-li1x1m25 411 15 not not RB cord-013178-li1x1m25 411 16 differentiate differentiate VB cord-013178-li1x1m25 411 17 from from IN cord-013178-li1x1m25 411 18 native native JJ cord-013178-li1x1m25 411 19 viral viral JJ cord-013178-li1x1m25 411 20 proteins protein NNS cord-013178-li1x1m25 411 21 or or CC cord-013178-li1x1m25 411 22 vaccine vaccine NN cord-013178-li1x1m25 411 23 ones one NNS cord-013178-li1x1m25 411 24 in in IN cord-013178-li1x1m25 411 25 blood blood NN cord-013178-li1x1m25 411 26 . . . cord-013178-li1x1m25 412 1 The the DT cord-013178-li1x1m25 412 2 ORF3 ORF3 NNP cord-013178-li1x1m25 412 3 protein protein NN cord-013178-li1x1m25 412 4 - - HYPH cord-013178-li1x1m25 412 5 ELISA ELISA NNP cord-013178-li1x1m25 412 6 , , , cord-013178-li1x1m25 412 7 by by IN cord-013178-li1x1m25 412 8 contrast contrast NN cord-013178-li1x1m25 412 9 , , , cord-013178-li1x1m25 412 10 only only RB cord-013178-li1x1m25 412 11 identified identify VBD cord-013178-li1x1m25 412 12 native native JJ cord-013178-li1x1m25 412 13 ORF3 ORF3 NNP cord-013178-li1x1m25 412 14 proteins protein NNS cord-013178-li1x1m25 412 15 in in IN cord-013178-li1x1m25 412 16 pig pig NN cord-013178-li1x1m25 412 17 blood blood NN cord-013178-li1x1m25 412 18 since since IN cord-013178-li1x1m25 412 19 no no DT cord-013178-li1x1m25 412 20 commercial commercial JJ cord-013178-li1x1m25 412 21 vaccine vaccine NN cord-013178-li1x1m25 412 22 contains contain VBZ cord-013178-li1x1m25 412 23 the the DT cord-013178-li1x1m25 412 24 ORF3 ORF3 NNP cord-013178-li1x1m25 412 25 protein protein NN cord-013178-li1x1m25 412 26 of of IN cord-013178-li1x1m25 412 27 PCV2 PCV2 NNP cord-013178-li1x1m25 412 28 . . . cord-013178-li1x1m25 413 1 For for IN cord-013178-li1x1m25 413 2 these these DT cord-013178-li1x1m25 413 3 purposes purpose NNS cord-013178-li1x1m25 413 4 , , , cord-013178-li1x1m25 413 5 this this DT cord-013178-li1x1m25 413 6 study study NN cord-013178-li1x1m25 413 7 used use VBD cord-013178-li1x1m25 413 8 the the DT cord-013178-li1x1m25 413 9 commercial commercial JJ cord-013178-li1x1m25 413 10 capsid capsid NN cord-013178-li1x1m25 413 11 antigen antigen NN cord-013178-li1x1m25 413 12 - - HYPH cord-013178-li1x1m25 413 13 ELISA ELISA NNP cord-013178-li1x1m25 413 14 and and CC cord-013178-li1x1m25 413 15 homemade homemade NNP cord-013178-li1x1m25 413 16 ORF3 ORF3 NNP cord-013178-li1x1m25 413 17 protein protein NN cord-013178-li1x1m25 413 18 - - HYPH cord-013178-li1x1m25 413 19 ELISA ELISA NNP cord-013178-li1x1m25 413 20 ( ( -LRB- cord-013178-li1x1m25 413 21 anti anti JJ cord-013178-li1x1m25 413 22 - - JJ cord-013178-li1x1m25 413 23 N1 n1 JJ cord-013178-li1x1m25 413 24 polyclonal polyclonal JJ cord-013178-li1x1m25 413 25 antibodies antibody NNS cord-013178-li1x1m25 413 26 and and CC cord-013178-li1x1m25 413 27 mAb mAb NNP cord-013178-li1x1m25 413 28 7D3 7d3 CD cord-013178-li1x1m25 413 29 based base VBN cord-013178-li1x1m25 413 30 ) ) -RRB- cord-013178-li1x1m25 413 31 to to TO cord-013178-li1x1m25 413 32 detect detect VB cord-013178-li1x1m25 413 33 viral viral JJ cord-013178-li1x1m25 413 34 proteins protein NNS cord-013178-li1x1m25 413 35 in in IN cord-013178-li1x1m25 413 36 pig pig NN cord-013178-li1x1m25 413 37 blood blood NN cord-013178-li1x1m25 413 38 . . . cord-013178-li1x1m25 414 1 This this DT cord-013178-li1x1m25 414 2 study study NN cord-013178-li1x1m25 414 3 found find VBD cord-013178-li1x1m25 414 4 non non JJ cord-013178-li1x1m25 414 5 - - JJ cord-013178-li1x1m25 414 6 vaccinated vaccinated JJ cord-013178-li1x1m25 414 7 suckers sucker NNS cord-013178-li1x1m25 414 8 had have VBD cord-013178-li1x1m25 414 9 the the DT cord-013178-li1x1m25 414 10 highest high JJS cord-013178-li1x1m25 414 11 of of IN cord-013178-li1x1m25 414 12 PCV2 pcv2 JJ cord-013178-li1x1m25 414 13 proteins protein NNS cord-013178-li1x1m25 414 14 in in IN cord-013178-li1x1m25 414 15 blood blood NN cord-013178-li1x1m25 414 16 at at IN cord-013178-li1x1m25 414 17 one one CD cord-013178-li1x1m25 414 18 week week NN cord-013178-li1x1m25 414 19 of of IN cord-013178-li1x1m25 414 20 age age NN cord-013178-li1x1m25 414 21 . . . cord-013178-li1x1m25 415 1 The the DT cord-013178-li1x1m25 415 2 author author NN cord-013178-li1x1m25 415 3 suggests suggest VBZ cord-013178-li1x1m25 415 4 that that IN cord-013178-li1x1m25 415 5 in in IN cord-013178-li1x1m25 415 6 these these DT cord-013178-li1x1m25 415 7 suckers sucker NNS cord-013178-li1x1m25 415 8 it -PRON- PRP cord-013178-li1x1m25 415 9 was be VBD cord-013178-li1x1m25 415 10 caused cause VBN cord-013178-li1x1m25 415 11 by by IN cord-013178-li1x1m25 415 12 PCV2 pcv2 JJ cord-013178-li1x1m25 415 13 infection infection NN cord-013178-li1x1m25 415 14 , , , cord-013178-li1x1m25 415 15 and and CC cord-013178-li1x1m25 415 16 these these DT cord-013178-li1x1m25 415 17 viruses virus NNS cord-013178-li1x1m25 415 18 majorly majorly RB cord-013178-li1x1m25 415 19 stemmed stem VBN cord-013178-li1x1m25 415 20 from from IN cord-013178-li1x1m25 415 21 the the DT cord-013178-li1x1m25 415 22 sow sow VB cord-013178-li1x1m25 415 23 - - HYPH cord-013178-li1x1m25 415 24 to to IN cord-013178-li1x1m25 415 25 - - HYPH cord-013178-li1x1m25 415 26 newborn newborn JJ cord-013178-li1x1m25 415 27 transmission transmission NN cord-013178-li1x1m25 415 28 [ [ -LRB- cord-013178-li1x1m25 415 29 68 68 CD cord-013178-li1x1m25 415 30 ] ] -RRB- cord-013178-li1x1m25 415 31 . . . cord-013178-li1x1m25 416 1 After after IN cord-013178-li1x1m25 416 2 inoculated inoculate VBN cord-013178-li1x1m25 416 3 with with IN cord-013178-li1x1m25 416 4 the the DT cord-013178-li1x1m25 416 5 PCV2 PCV2 NNP cord-013178-li1x1m25 416 6 subunit subunit NN cord-013178-li1x1m25 416 7 vaccine vaccine NN cord-013178-li1x1m25 416 8 , , , cord-013178-li1x1m25 416 9 the the DT cord-013178-li1x1m25 416 10 capsid capsid NN cord-013178-li1x1m25 416 11 protein protein NN cord-013178-li1x1m25 416 12 , , , cord-013178-li1x1m25 416 13 and and CC cord-013178-li1x1m25 416 14 the the DT cord-013178-li1x1m25 416 15 ORF3 ORF3 NNP cord-013178-li1x1m25 416 16 protein protein NN cord-013178-li1x1m25 416 17 were be VBD cord-013178-li1x1m25 416 18 gradually gradually RB cord-013178-li1x1m25 416 19 decreased decrease VBN cord-013178-li1x1m25 416 20 . . . cord-013178-li1x1m25 417 1 However however RB cord-013178-li1x1m25 417 2 , , , cord-013178-li1x1m25 417 3 these these DT cord-013178-li1x1m25 417 4 proteins protein NNS cord-013178-li1x1m25 417 5 were be VBD cord-013178-li1x1m25 417 6 detected detect VBN cord-013178-li1x1m25 417 7 again again RB cord-013178-li1x1m25 417 8 in in IN cord-013178-li1x1m25 417 9 gilts gilt NNS cord-013178-li1x1m25 417 10 and and CC cord-013178-li1x1m25 417 11 sows sow NNS cord-013178-li1x1m25 417 12 ( ( -LRB- cord-013178-li1x1m25 417 13 ≥34 ≥34 VBZ cord-013178-li1x1m25 417 14 weeks week NNS cord-013178-li1x1m25 417 15 of of IN cord-013178-li1x1m25 417 16 age age NN cord-013178-li1x1m25 417 17 ) ) -RRB- cord-013178-li1x1m25 417 18 . . . cord-013178-li1x1m25 418 1 That that DT cord-013178-li1x1m25 418 2 means mean VBZ cord-013178-li1x1m25 418 3 PCV2 pcv2 JJ cord-013178-li1x1m25 418 4 viral viral JJ cord-013178-li1x1m25 418 5 proteins protein NNS cord-013178-li1x1m25 418 6 ( ( -LRB- cord-013178-li1x1m25 418 7 the the DT cord-013178-li1x1m25 418 8 capsid capsid NN cord-013178-li1x1m25 418 9 protein protein NN cord-013178-li1x1m25 418 10 or or CC cord-013178-li1x1m25 418 11 the the DT cord-013178-li1x1m25 418 12 ORF3 ORF3 NNP cord-013178-li1x1m25 418 13 protein protein NN cord-013178-li1x1m25 418 14 ) ) -RRB- cord-013178-li1x1m25 418 15 in in IN cord-013178-li1x1m25 418 16 gilts gilt NNS cord-013178-li1x1m25 418 17 and and CC cord-013178-li1x1m25 418 18 sows sow NNS cord-013178-li1x1m25 418 19 were be VBD cord-013178-li1x1m25 418 20 higher high JJR cord-013178-li1x1m25 418 21 than than IN cord-013178-li1x1m25 418 22 that that DT cord-013178-li1x1m25 418 23 of of IN cord-013178-li1x1m25 418 24 pigs pig NNS cord-013178-li1x1m25 418 25 at at IN cord-013178-li1x1m25 418 26 9 9 CD cord-013178-li1x1m25 418 27 - - SYM cord-013178-li1x1m25 418 28 24 24 CD cord-013178-li1x1m25 418 29 weeks week NNS cord-013178-li1x1m25 418 30 of of IN cord-013178-li1x1m25 418 31 age age NN cord-013178-li1x1m25 418 32 . . . cord-013178-li1x1m25 419 1 To to TO cord-013178-li1x1m25 419 2 put put VB cord-013178-li1x1m25 419 3 it -PRON- PRP cord-013178-li1x1m25 419 4 another another DT cord-013178-li1x1m25 419 5 way way NN cord-013178-li1x1m25 419 6 , , , cord-013178-li1x1m25 419 7 vaccinated vaccinate VBN cord-013178-li1x1m25 419 8 - - HYPH cord-013178-li1x1m25 419 9 pigs pig NNS cord-013178-li1x1m25 419 10 reduced reduce VBN cord-013178-li1x1m25 419 11 viremia viremia NN cord-013178-li1x1m25 419 12 compared compare VBN cord-013178-li1x1m25 419 13 to to IN cord-013178-li1x1m25 419 14 non non JJ cord-013178-li1x1m25 419 15 - - JJ cord-013178-li1x1m25 419 16 vaccinated vaccinated JJ cord-013178-li1x1m25 419 17 suckers sucker NNS cord-013178-li1x1m25 419 18 , , , cord-013178-li1x1m25 419 19 and and CC cord-013178-li1x1m25 419 20 the the DT cord-013178-li1x1m25 419 21 immunity immunity NN cord-013178-li1x1m25 419 22 could could MD cord-013178-li1x1m25 419 23 not not RB cord-013178-li1x1m25 419 24 continue continue VB cord-013178-li1x1m25 419 25 to to TO cord-013178-li1x1m25 419 26 protect protect VB cord-013178-li1x1m25 419 27 vaccinated vaccinated JJ cord-013178-li1x1m25 419 28 pigs pig NNS cord-013178-li1x1m25 419 29 after after IN cord-013178-li1x1m25 419 30 32 32 CD cord-013178-li1x1m25 419 31 weeks week NNS cord-013178-li1x1m25 419 32 post post NN cord-013178-li1x1m25 419 33 - - NN cord-013178-li1x1m25 419 34 vaccination vaccination NN cord-013178-li1x1m25 419 35 . . . cord-013178-li1x1m25 420 1 Since since IN cord-013178-li1x1m25 420 2 PCV2 PCV2 NNP cord-013178-li1x1m25 420 3 is be VBZ cord-013178-li1x1m25 420 4 highly highly RB cord-013178-li1x1m25 420 5 resistant resistant JJ cord-013178-li1x1m25 420 6 to to IN cord-013178-li1x1m25 420 7 environmental environmental JJ cord-013178-li1x1m25 420 8 conditions condition NNS cord-013178-li1x1m25 420 9 , , , cord-013178-li1x1m25 420 10 being be VBG cord-013178-li1x1m25 420 11 able able JJ cord-013178-li1x1m25 420 12 to to TO cord-013178-li1x1m25 420 13 remain remain VB cord-013178-li1x1m25 420 14 in in IN cord-013178-li1x1m25 420 15 the the DT cord-013178-li1x1m25 420 16 farm farm NN cord-013178-li1x1m25 420 17 environment environment NN cord-013178-li1x1m25 420 18 and and CC cord-013178-li1x1m25 420 19 thus thus RB cord-013178-li1x1m25 420 20 represent represent VB cord-013178-li1x1m25 420 21 a a DT cord-013178-li1x1m25 420 22 risk risk NN cord-013178-li1x1m25 420 23 for for IN cord-013178-li1x1m25 420 24 infection infection NN cord-013178-li1x1m25 420 25 maintenance maintenance NN cord-013178-li1x1m25 420 26 [ [ -LRB- cord-013178-li1x1m25 420 27 69 69 CD cord-013178-li1x1m25 420 28 ] ] -RRB- cord-013178-li1x1m25 420 29 , , , cord-013178-li1x1m25 420 30 even even RB cord-013178-li1x1m25 420 31 mass mass JJ cord-013178-li1x1m25 420 32 PCV2 pcv2 JJ cord-013178-li1x1m25 420 33 vaccination vaccination NN cord-013178-li1x1m25 420 34 ( ( -LRB- cord-013178-li1x1m25 420 35 without without IN cord-013178-li1x1m25 420 36 implementing implement VBG cord-013178-li1x1m25 420 37 further further JJ cord-013178-li1x1m25 420 38 farm farm NN cord-013178-li1x1m25 420 39 management management NN cord-013178-li1x1m25 420 40 practices practice NNS cord-013178-li1x1m25 420 41 or or CC cord-013178-li1x1m25 420 42 biosafety biosafety NN cord-013178-li1x1m25 420 43 measures measure NNS cord-013178-li1x1m25 420 44 ) ) -RRB- cord-013178-li1x1m25 420 45 was be VBD cord-013178-li1x1m25 420 46 not not RB cord-013178-li1x1m25 420 47 able able JJ cord-013178-li1x1m25 420 48 to to TO cord-013178-li1x1m25 420 49 clear clear VB cord-013178-li1x1m25 420 50 out out RP cord-013178-li1x1m25 420 51 PCV2 pcv2 JJ cord-013178-li1x1m25 420 52 infection infection NN cord-013178-li1x1m25 420 53 , , , cord-013178-li1x1m25 420 54 and and CC cord-013178-li1x1m25 420 55 the the DT cord-013178-li1x1m25 420 56 virus virus NN cord-013178-li1x1m25 420 57 became become VBD cord-013178-li1x1m25 420 58 detectable detectable JJ cord-013178-li1x1m25 420 59 again again RB cord-013178-li1x1m25 420 60 when when WRB cord-013178-li1x1m25 420 61 vaccination vaccination NN cord-013178-li1x1m25 420 62 was be VBD cord-013178-li1x1m25 420 63 stopped stop VBN cord-013178-li1x1m25 420 64 [ [ -LRB- cord-013178-li1x1m25 420 65 12 12 CD cord-013178-li1x1m25 420 66 , , , cord-013178-li1x1m25 420 67 67 67 CD cord-013178-li1x1m25 420 68 ] ] -RRB- cord-013178-li1x1m25 420 69 . . . cord-013178-li1x1m25 421 1 Another another DT cord-013178-li1x1m25 421 2 key key JJ cord-013178-li1x1m25 421 3 point point NN cord-013178-li1x1m25 421 4 to to TO cord-013178-li1x1m25 421 5 mention mention VB cord-013178-li1x1m25 421 6 is be VBZ cord-013178-li1x1m25 421 7 that that IN cord-013178-li1x1m25 421 8 PCV2-specific PCV2-specific NNP cord-013178-li1x1m25 421 9 antibodies antibody NNS cord-013178-li1x1m25 421 10 response response NN cord-013178-li1x1m25 421 11 play play VBP cord-013178-li1x1m25 421 12 roles role NNS cord-013178-li1x1m25 421 13 in in IN cord-013178-li1x1m25 421 14 the the DT cord-013178-li1x1m25 421 15 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 421 16 herd herd NN cord-013178-li1x1m25 421 17 . . . cord-013178-li1x1m25 422 1 It -PRON- PRP cord-013178-li1x1m25 422 2 is be VBZ cord-013178-li1x1m25 422 3 worth worth JJ cord-013178-li1x1m25 422 4 noting note VBG cord-013178-li1x1m25 422 5 that that IN cord-013178-li1x1m25 422 6 there there EX cord-013178-li1x1m25 422 7 were be VBD cord-013178-li1x1m25 422 8 two two CD cord-013178-li1x1m25 422 9 different different JJ cord-013178-li1x1m25 422 10 antibody antibody NN cord-013178-li1x1m25 422 11 profiles profile NNS cord-013178-li1x1m25 422 12 in in IN cord-013178-li1x1m25 422 13 this this DT cord-013178-li1x1m25 422 14 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 422 15 herd herd NN cord-013178-li1x1m25 422 16 with with IN cord-013178-li1x1m25 422 17 the the DT cord-013178-li1x1m25 422 18 PCV2 PCV2 NNP cord-013178-li1x1m25 422 19 vaccine vaccine NN cord-013178-li1x1m25 422 20 . . . cord-013178-li1x1m25 423 1 The the DT cord-013178-li1x1m25 423 2 data datum NNS cord-013178-li1x1m25 423 3 show show VBP cord-013178-li1x1m25 423 4 that that IN cord-013178-li1x1m25 423 5 PCV2-infected PCV2-infected NNP cord-013178-li1x1m25 423 6 herd herd NN cord-013178-li1x1m25 423 7 had have VBD cord-013178-li1x1m25 423 8 a a DT cord-013178-li1x1m25 423 9 higher high JJR cord-013178-li1x1m25 423 10 OD OD NNP cord-013178-li1x1m25 423 11 405 405 CD cord-013178-li1x1m25 423 12 value value NN cord-013178-li1x1m25 423 13 of of IN cord-013178-li1x1m25 423 14 anti anti JJ cord-013178-li1x1m25 423 15 - - JJ cord-013178-li1x1m25 423 16 capsid capsid JJ cord-013178-li1x1m25 423 17 IgG igg NN cord-013178-li1x1m25 423 18 at at IN cord-013178-li1x1m25 423 19 age age NN cord-013178-li1x1m25 423 20 1 1 CD cord-013178-li1x1m25 423 21 , , , cord-013178-li1x1m25 423 22 3 3 CD cord-013178-li1x1m25 423 23 , , , cord-013178-li1x1m25 423 24 and and CC cord-013178-li1x1m25 423 25 ≥12 ≥12 NNP cord-013178-li1x1m25 423 26 weeks week NNS cord-013178-li1x1m25 423 27 , , , cord-013178-li1x1m25 423 28 compared compare VBN cord-013178-li1x1m25 423 29 with with IN cord-013178-li1x1m25 423 30 6 6 CD cord-013178-li1x1m25 423 31 - - SYM cord-013178-li1x1m25 423 32 9 9 CD cord-013178-li1x1m25 423 33 weeks week NNS cord-013178-li1x1m25 423 34 . . . cord-013178-li1x1m25 424 1 In in IN cord-013178-li1x1m25 424 2 addition addition NN cord-013178-li1x1m25 424 3 , , , cord-013178-li1x1m25 424 4 PCV2-infected PCV2-infected NNP cord-013178-li1x1m25 424 5 herd herd NN cord-013178-li1x1m25 424 6 had have VBD cord-013178-li1x1m25 424 7 a a DT cord-013178-li1x1m25 424 8 higher high JJR cord-013178-li1x1m25 424 9 OD OD NNP cord-013178-li1x1m25 424 10 405 405 CD cord-013178-li1x1m25 424 11 value value NN cord-013178-li1x1m25 424 12 of of IN cord-013178-li1x1m25 424 13 anti anti JJ cord-013178-li1x1m25 424 14 - - JJ cord-013178-li1x1m25 424 15 ORF3 orf3 JJ cord-013178-li1x1m25 424 16 IgG igg NN cord-013178-li1x1m25 424 17 at at IN cord-013178-li1x1m25 424 18 age age NN cord-013178-li1x1m25 424 19 1 1 CD cord-013178-li1x1m25 424 20 and and CC cord-013178-li1x1m25 424 21 ≥9 ≥9 NNPS cord-013178-li1x1m25 424 22 weeks week NNS cord-013178-li1x1m25 424 23 , , , cord-013178-li1x1m25 424 24 compared compare VBN cord-013178-li1x1m25 424 25 with with IN cord-013178-li1x1m25 424 26 3 3 CD cord-013178-li1x1m25 424 27 - - SYM cord-013178-li1x1m25 424 28 6 6 CD cord-013178-li1x1m25 424 29 weeks week NNS cord-013178-li1x1m25 424 30 . . . cord-013178-li1x1m25 425 1 According accord VBG cord-013178-li1x1m25 425 2 to to IN cord-013178-li1x1m25 425 3 previous previous JJ cord-013178-li1x1m25 425 4 studies study NNS cord-013178-li1x1m25 425 5 , , , cord-013178-li1x1m25 425 6 newborn newborn JJ cord-013178-li1x1m25 425 7 suckers sucker NNS cord-013178-li1x1m25 425 8 received receive VBD cord-013178-li1x1m25 425 9 colostral colostral JJ cord-013178-li1x1m25 425 10 antibodies antibody NNS cord-013178-li1x1m25 425 11 from from IN cord-013178-li1x1m25 425 12 seropositive seropositive JJ cord-013178-li1x1m25 425 13 sows sow NNS cord-013178-li1x1m25 425 14 and and CC cord-013178-li1x1m25 425 15 showed show VBD cord-013178-li1x1m25 425 16 various various JJ cord-013178-li1x1m25 425 17 levels level NNS cord-013178-li1x1m25 425 18 of of IN cord-013178-li1x1m25 425 19 maternally maternally RB cord-013178-li1x1m25 425 20 derived derive VBN cord-013178-li1x1m25 425 21 antibodies antibody NNS cord-013178-li1x1m25 425 22 [ [ -LRB- cord-013178-li1x1m25 425 23 48 48 CD cord-013178-li1x1m25 425 24 , , , cord-013178-li1x1m25 425 25 68 68 CD cord-013178-li1x1m25 425 26 ] ] -RRB- cord-013178-li1x1m25 425 27 . . . cord-013178-li1x1m25 426 1 These these DT cord-013178-li1x1m25 426 2 maternally maternally RB cord-013178-li1x1m25 426 3 derived derive VBN cord-013178-li1x1m25 426 4 antibodies antibody NNS cord-013178-li1x1m25 426 5 might may MD cord-013178-li1x1m25 426 6 interfere interfere VB cord-013178-li1x1m25 426 7 with with IN cord-013178-li1x1m25 426 8 the the DT cord-013178-li1x1m25 426 9 viral viral JJ cord-013178-li1x1m25 426 10 protein protein NN cord-013178-li1x1m25 426 11 - - HYPH cord-013178-li1x1m25 426 12 specific specific JJ cord-013178-li1x1m25 426 13 antibodies antibody NNS cord-013178-li1x1m25 426 14 response response NN cord-013178-li1x1m25 426 15 while while IN cord-013178-li1x1m25 426 16 suckers sucker NNS cord-013178-li1x1m25 426 17 immunized immunize VBN cord-013178-li1x1m25 426 18 with with IN cord-013178-li1x1m25 426 19 the the DT cord-013178-li1x1m25 426 20 PCV2 PCV2 NNP cord-013178-li1x1m25 426 21 subunit subunit NN cord-013178-li1x1m25 426 22 vaccine vaccine NN cord-013178-li1x1m25 426 23 . . . cord-013178-li1x1m25 427 1 It -PRON- PRP cord-013178-li1x1m25 427 2 may may MD cord-013178-li1x1m25 427 3 be be VB cord-013178-li1x1m25 427 4 assumed assume VBN cord-013178-li1x1m25 427 5 that that IN cord-013178-li1x1m25 427 6 the the DT cord-013178-li1x1m25 427 7 artificial artificial JJ cord-013178-li1x1m25 427 8 capsid capsid NN cord-013178-li1x1m25 427 9 protein protein NN cord-013178-li1x1m25 427 10 ( ( -LRB- cord-013178-li1x1m25 427 11 PCV2 pcv2 JJ cord-013178-li1x1m25 427 12 vaccine vaccine NN cord-013178-li1x1m25 427 13 ) ) -RRB- cord-013178-li1x1m25 427 14 elicited elicit VBD cord-013178-li1x1m25 427 15 anti anti JJ cord-013178-li1x1m25 427 16 - - JJ cord-013178-li1x1m25 427 17 capsid capsid JJ cord-013178-li1x1m25 427 18 IgG igg NN cord-013178-li1x1m25 427 19 producing produce VBG cord-013178-li1x1m25 427 20 while while IN cord-013178-li1x1m25 427 21 maternally maternally RB cord-013178-li1x1m25 427 22 derived derive VBN cord-013178-li1x1m25 427 23 antibodies antibody NNS cord-013178-li1x1m25 427 24 progressed progress VBD cord-013178-li1x1m25 427 25 to to TO cord-013178-li1x1m25 427 26 degrade degrade VB cord-013178-li1x1m25 427 27 . . . cord-013178-li1x1m25 428 1 The the DT cord-013178-li1x1m25 428 2 total total JJ cord-013178-li1x1m25 428 3 level level NN cord-013178-li1x1m25 428 4 of of IN cord-013178-li1x1m25 428 5 anti anti JJ cord-013178-li1x1m25 428 6 - - JJ cord-013178-li1x1m25 428 7 capsid capsid JJ cord-013178-li1x1m25 428 8 IgG igg NN cord-013178-li1x1m25 428 9 decreased decrease VBD cord-013178-li1x1m25 428 10 slowly slowly RB cord-013178-li1x1m25 428 11 but but CC cord-013178-li1x1m25 428 12 still still RB cord-013178-li1x1m25 428 13 maintained maintain VBD cord-013178-li1x1m25 428 14 a a DT cord-013178-li1x1m25 428 15 high high JJ cord-013178-li1x1m25 428 16 level level NN cord-013178-li1x1m25 428 17 of of IN cord-013178-li1x1m25 428 18 anti anti JJ cord-013178-li1x1m25 428 19 - - JJ cord-013178-li1x1m25 428 20 capsid capsid JJ cord-013178-li1x1m25 428 21 IgG igg NN cord-013178-li1x1m25 428 22 during during IN cord-013178-li1x1m25 428 23 the the DT cord-013178-li1x1m25 428 24 age age NN cord-013178-li1x1m25 428 25 of of IN cord-013178-li1x1m25 428 26 1 1 CD cord-013178-li1x1m25 428 27 - - SYM cord-013178-li1x1m25 428 28 6 6 CD cord-013178-li1x1m25 428 29 weeks week NNS cord-013178-li1x1m25 428 30 . . . cord-013178-li1x1m25 429 1 This this DT cord-013178-li1x1m25 429 2 phenomenon phenomenon NN cord-013178-li1x1m25 429 3 could could MD cord-013178-li1x1m25 429 4 neutralize neutralize VB cord-013178-li1x1m25 429 5 the the DT cord-013178-li1x1m25 429 6 PCV2 PCV2 NNP cord-013178-li1x1m25 429 7 virus virus NN cord-013178-li1x1m25 429 8 and and CC cord-013178-li1x1m25 429 9 prevent prevent VB cord-013178-li1x1m25 429 10 piglets piglet NNS cord-013178-li1x1m25 429 11 from from IN cord-013178-li1x1m25 429 12 suffering suffer VBG cord-013178-li1x1m25 429 13 PCV2 pcv2 JJ cord-013178-li1x1m25 429 14 infection infection NN cord-013178-li1x1m25 429 15 . . . cord-013178-li1x1m25 430 1 In in IN cord-013178-li1x1m25 430 2 contrast contrast NN cord-013178-li1x1m25 430 3 with with IN cord-013178-li1x1m25 430 4 dual dual JJ cord-013178-li1x1m25 430 5 - - HYPH cord-013178-li1x1m25 430 6 source source NN cord-013178-li1x1m25 430 7 capsid capsid NN cord-013178-li1x1m25 430 8 proteins protein NNS cord-013178-li1x1m25 430 9 ( ( -LRB- cord-013178-li1x1m25 431 1 PCV2 pcv2 JJ cord-013178-li1x1m25 431 2 virus virus NN cord-013178-li1x1m25 431 3 and and CC cord-013178-li1x1m25 431 4 subunit subunit NN cord-013178-li1x1m25 431 5 vaccine vaccine NN cord-013178-li1x1m25 431 6 ) ) -RRB- cord-013178-li1x1m25 431 7 , , , cord-013178-li1x1m25 431 8 the the DT cord-013178-li1x1m25 431 9 piglet piglet NN cord-013178-li1x1m25 431 10 's 's POS cord-013178-li1x1m25 431 11 immunity immunity NN cord-013178-li1x1m25 431 12 response response NN cord-013178-li1x1m25 431 13 to to IN cord-013178-li1x1m25 431 14 the the DT cord-013178-li1x1m25 431 15 ORF3 ORF3 NNP cord-013178-li1x1m25 431 16 protein protein NN cord-013178-li1x1m25 431 17 was be VBD cord-013178-li1x1m25 431 18 only only RB cord-013178-li1x1m25 431 19 caused cause VBN cord-013178-li1x1m25 431 20 by by IN cord-013178-li1x1m25 431 21 PCV2 pcv2 JJ cord-013178-li1x1m25 431 22 virus virus NN cord-013178-li1x1m25 431 23 infection infection NN cord-013178-li1x1m25 431 24 . . . cord-013178-li1x1m25 432 1 The the DT cord-013178-li1x1m25 432 2 total total JJ cord-013178-li1x1m25 432 3 level level NN cord-013178-li1x1m25 432 4 of of IN cord-013178-li1x1m25 432 5 anti anti JJ cord-013178-li1x1m25 432 6 - - JJ cord-013178-li1x1m25 432 7 ORF3 orf3 JJ cord-013178-li1x1m25 432 8 IgG igg NN cord-013178-li1x1m25 432 9 decreased decrease VBD cord-013178-li1x1m25 432 10 quickly quickly RB cord-013178-li1x1m25 432 11 at at IN cord-013178-li1x1m25 432 12 1 1 CD cord-013178-li1x1m25 432 13 - - SYM cord-013178-li1x1m25 432 14 3 3 CD cord-013178-li1x1m25 432 15 weeks week NNS cord-013178-li1x1m25 432 16 of of IN cord-013178-li1x1m25 432 17 age age NN cord-013178-li1x1m25 432 18 . . . cord-013178-li1x1m25 433 1 Then then RB cord-013178-li1x1m25 433 2 , , , cord-013178-li1x1m25 433 3 it -PRON- PRP cord-013178-li1x1m25 433 4 increased increase VBD cord-013178-li1x1m25 433 5 significantly significantly RB cord-013178-li1x1m25 433 6 at at IN cord-013178-li1x1m25 433 7 6 6 CD cord-013178-li1x1m25 433 8 - - SYM cord-013178-li1x1m25 433 9 9 9 CD cord-013178-li1x1m25 433 10 weeks week NNS cord-013178-li1x1m25 433 11 of of IN cord-013178-li1x1m25 433 12 age age NN cord-013178-li1x1m25 433 13 . . . cord-013178-li1x1m25 434 1 The the DT cord-013178-li1x1m25 434 2 weak weak JJ cord-013178-li1x1m25 434 3 correlation correlation NN cord-013178-li1x1m25 434 4 ( ( -LRB- cord-013178-li1x1m25 434 5 r r NN cord-013178-li1x1m25 434 6 = = SYM cord-013178-li1x1m25 434 7 0.3771 0.3771 CD cord-013178-li1x1m25 434 8 ) ) -RRB- cord-013178-li1x1m25 434 9 between between IN cord-013178-li1x1m25 434 10 the the DT cord-013178-li1x1m25 434 11 anti anti JJ cord-013178-li1x1m25 434 12 - - JJ cord-013178-li1x1m25 434 13 capsid capsid JJ cord-013178-li1x1m25 434 14 IgG igg NN cord-013178-li1x1m25 434 15 and and CC cord-013178-li1x1m25 434 16 the the DT cord-013178-li1x1m25 434 17 anti anti NNP cord-013178-li1x1m25 434 18 - - NNP cord-013178-li1x1m25 434 19 ORF3 orf3 JJ cord-013178-li1x1m25 434 20 IgG igg NN cord-013178-li1x1m25 434 21 is be VBZ cord-013178-li1x1m25 434 22 worth worth JJ cord-013178-li1x1m25 434 23 mentioning mention VBG cord-013178-li1x1m25 434 24 . . . cord-013178-li1x1m25 435 1 It -PRON- PRP cord-013178-li1x1m25 435 2 might may MD cord-013178-li1x1m25 435 3 reflect reflect VB cord-013178-li1x1m25 435 4 two two CD cord-013178-li1x1m25 435 5 kinds kind NNS cord-013178-li1x1m25 435 6 of of IN cord-013178-li1x1m25 435 7 antibody antibody NN cord-013178-li1x1m25 435 8 responses response NNS cord-013178-li1x1m25 435 9 in in IN cord-013178-li1x1m25 435 10 virus virus NN cord-013178-li1x1m25 435 11 infection infection NN cord-013178-li1x1m25 435 12 and and CC cord-013178-li1x1m25 435 13 post post NN cord-013178-li1x1m25 435 14 - - NN cord-013178-li1x1m25 435 15 immunization immunization NN cord-013178-li1x1m25 435 16 . . . cord-013178-li1x1m25 436 1 These these DT cord-013178-li1x1m25 436 2 data datum NNS cord-013178-li1x1m25 436 3 interpret interpret VBP cord-013178-li1x1m25 436 4 the the DT cord-013178-li1x1m25 436 5 interaction interaction NN cord-013178-li1x1m25 436 6 of of IN cord-013178-li1x1m25 436 7 the the DT cord-013178-li1x1m25 436 8 viral viral JJ cord-013178-li1x1m25 436 9 protein protein NN cord-013178-li1x1m25 436 10 with with IN cord-013178-li1x1m25 436 11 the the DT cord-013178-li1x1m25 436 12 host host NN cord-013178-li1x1m25 436 13 immune immune JJ cord-013178-li1x1m25 436 14 system system NN cord-013178-li1x1m25 436 15 . . . cord-013178-li1x1m25 437 1 According accord VBG cord-013178-li1x1m25 437 2 to to IN cord-013178-li1x1m25 437 3 previous previous JJ cord-013178-li1x1m25 437 4 reports report NNS cord-013178-li1x1m25 437 5 , , , cord-013178-li1x1m25 437 6 ORF2 ORF2 NNP cord-013178-li1x1m25 437 7 encodes encode VBZ cord-013178-li1x1m25 437 8 a a DT cord-013178-li1x1m25 437 9 30-kDa 30-kda CD cord-013178-li1x1m25 437 10 protein protein NN cord-013178-li1x1m25 437 11 that that WDT cord-013178-li1x1m25 437 12 is be VBZ cord-013178-li1x1m25 437 13 involved involve VBN cord-013178-li1x1m25 437 14 in in IN cord-013178-li1x1m25 437 15 viral viral JJ cord-013178-li1x1m25 437 16 capsid capsid NN cord-013178-li1x1m25 437 17 formation formation NN cord-013178-li1x1m25 437 18 and and CC cord-013178-li1x1m25 437 19 contributed contribute VBD cord-013178-li1x1m25 437 20 to to IN cord-013178-li1x1m25 437 21 self self NN cord-013178-li1x1m25 437 22 - - HYPH cord-013178-li1x1m25 437 23 assembled assemble VBN cord-013178-li1x1m25 437 24 virus virus NN cord-013178-li1x1m25 437 25 - - HYPH cord-013178-li1x1m25 437 26 like like JJ cord-013178-li1x1m25 437 27 particles particle NNS cord-013178-li1x1m25 437 28 ( ( -LRB- cord-013178-li1x1m25 437 29 VLP VLP NNP cord-013178-li1x1m25 437 30 ) ) -RRB- cord-013178-li1x1m25 437 31 [ [ -LRB- cord-013178-li1x1m25 437 32 32 32 CD cord-013178-li1x1m25 437 33 ] ] -RRB- cord-013178-li1x1m25 437 34 . . . cord-013178-li1x1m25 438 1 The the DT cord-013178-li1x1m25 438 2 monomer monomer NN cord-013178-li1x1m25 438 3 structures structure NNS cord-013178-li1x1m25 438 4 were be VBD cord-013178-li1x1m25 438 5 assembled assemble VBN cord-013178-li1x1m25 438 6 into into IN cord-013178-li1x1m25 438 7 a a DT cord-013178-li1x1m25 438 8 VLP VLP NNP cord-013178-li1x1m25 438 9 model model NN cord-013178-li1x1m25 438 10 consisting consist VBG cord-013178-li1x1m25 438 11 of of IN cord-013178-li1x1m25 438 12 60 60 CD cord-013178-li1x1m25 438 13 capsid capsid NN cord-013178-li1x1m25 438 14 subunits subunit NNS cord-013178-li1x1m25 438 15 to to TO cord-013178-li1x1m25 438 16 form form VB cord-013178-li1x1m25 438 17 an an DT cord-013178-li1x1m25 438 18 icosahedron icosahedron NN cord-013178-li1x1m25 438 19 [ [ -LRB- cord-013178-li1x1m25 438 20 70 70 CD cord-013178-li1x1m25 438 21 ] ] -RRB- cord-013178-li1x1m25 438 22 . . . cord-013178-li1x1m25 439 1 Vaccination vaccination NN cord-013178-li1x1m25 439 2 with with IN cord-013178-li1x1m25 439 3 this this DT cord-013178-li1x1m25 439 4 VLP VLP NNP cord-013178-li1x1m25 439 5 ( ( -LRB- cord-013178-li1x1m25 439 6 subunit subunit NN cord-013178-li1x1m25 439 7 vaccine vaccine NN cord-013178-li1x1m25 439 8 ) ) -RRB- cord-013178-li1x1m25 439 9 induced induce VBN cord-013178-li1x1m25 439 10 both both CC cord-013178-li1x1m25 439 11 humoral humoral JJ cord-013178-li1x1m25 439 12 and and CC cord-013178-li1x1m25 439 13 cell cell NN cord-013178-li1x1m25 439 14 - - HYPH cord-013178-li1x1m25 439 15 mediated mediate VBN cord-013178-li1x1m25 439 16 responses response NNS cord-013178-li1x1m25 439 17 against against IN cord-013178-li1x1m25 439 18 PCV2 PCV2 NNP cord-013178-li1x1m25 439 19 [ [ -LRB- cord-013178-li1x1m25 439 20 71 71 CD cord-013178-li1x1m25 439 21 , , , cord-013178-li1x1m25 439 22 72 72 CD cord-013178-li1x1m25 439 23 ] ] -RRB- cord-013178-li1x1m25 439 24 . . . cord-013178-li1x1m25 440 1 However however RB cord-013178-li1x1m25 440 2 , , , cord-013178-li1x1m25 440 3 ORF3 ORF3 NNP cord-013178-li1x1m25 440 4 encodes encode VBZ cord-013178-li1x1m25 440 5 an an DT cord-013178-li1x1m25 440 6 11.9-kDa 11.9-kda CD cord-013178-li1x1m25 440 7 protein protein NN cord-013178-li1x1m25 440 8 [ [ -LRB- cord-013178-li1x1m25 440 9 28 28 CD cord-013178-li1x1m25 440 10 ] ] -RRB- cord-013178-li1x1m25 440 11 that that WDT cord-013178-li1x1m25 440 12 is be VBZ cord-013178-li1x1m25 440 13 the the DT cord-013178-li1x1m25 440 14 non non JJ cord-013178-li1x1m25 440 15 - - JJ cord-013178-li1x1m25 440 16 structural structural JJ cord-013178-li1x1m25 440 17 protein protein NN cord-013178-li1x1m25 440 18 and and CC cord-013178-li1x1m25 440 19 involved involve VBN cord-013178-li1x1m25 440 20 in in IN cord-013178-li1x1m25 440 21 PCV2-induced pcv2-induced JJ cord-013178-li1x1m25 440 22 apoptosis apoptosis NN cord-013178-li1x1m25 440 23 [ [ -LRB- cord-013178-li1x1m25 440 24 34 34 CD cord-013178-li1x1m25 440 25 , , , cord-013178-li1x1m25 440 26 37 37 CD cord-013178-li1x1m25 440 27 , , , cord-013178-li1x1m25 440 28 42 42 CD cord-013178-li1x1m25 440 29 ] ] -RRB- cord-013178-li1x1m25 440 30 . . . cord-013178-li1x1m25 441 1 Until until IN cord-013178-li1x1m25 441 2 now now RB cord-013178-li1x1m25 441 3 , , , cord-013178-li1x1m25 441 4 no no DT cord-013178-li1x1m25 441 5 report report NN cord-013178-li1x1m25 441 6 mentions mention VBZ cord-013178-li1x1m25 441 7 that that IN cord-013178-li1x1m25 441 8 the the DT cord-013178-li1x1m25 441 9 ORF3 ORF3 NNP cord-013178-li1x1m25 441 10 protein protein NN cord-013178-li1x1m25 441 11 was be VBD cord-013178-li1x1m25 441 12 solely solely RB cord-013178-li1x1m25 441 13 used use VBN cord-013178-li1x1m25 441 14 as as IN cord-013178-li1x1m25 441 15 the the DT cord-013178-li1x1m25 441 16 vaccine vaccine NN cord-013178-li1x1m25 441 17 against against IN cord-013178-li1x1m25 441 18 PCV2 PCV2 NNP cord-013178-li1x1m25 441 19 , , , cord-013178-li1x1m25 441 20 reducing reduce VBG cord-013178-li1x1m25 441 21 viremia viremia NN cord-013178-li1x1m25 441 22 or or CC cord-013178-li1x1m25 441 23 pathological pathological JJ cord-013178-li1x1m25 441 24 lesions lesion NNS cord-013178-li1x1m25 441 25 in in IN cord-013178-li1x1m25 441 26 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 441 27 herds herd NNS cord-013178-li1x1m25 441 28 . . . cord-013178-li1x1m25 442 1 These these DT cord-013178-li1x1m25 442 2 differences difference NNS cord-013178-li1x1m25 442 3 contributed contribute VBD cord-013178-li1x1m25 442 4 to to IN cord-013178-li1x1m25 442 5 their -PRON- PRP$ cord-013178-li1x1m25 442 6 application application NN cord-013178-li1x1m25 442 7 in in IN cord-013178-li1x1m25 442 8 different different JJ cord-013178-li1x1m25 442 9 ways way NNS cord-013178-li1x1m25 442 10 [ [ -LRB- cord-013178-li1x1m25 442 11 36 36 CD cord-013178-li1x1m25 442 12 , , , cord-013178-li1x1m25 442 13 73 73 CD cord-013178-li1x1m25 442 14 ] ] -RRB- cord-013178-li1x1m25 442 15 . . . cord-013178-li1x1m25 443 1 In in IN cord-013178-li1x1m25 443 2 this this DT cord-013178-li1x1m25 443 3 study study NN cord-013178-li1x1m25 443 4 , , , cord-013178-li1x1m25 443 5 the the DT cord-013178-li1x1m25 443 6 percentage percentage NN cord-013178-li1x1m25 443 7 of of IN cord-013178-li1x1m25 443 8 ORF3-positive ORF3-positive NNP cord-013178-li1x1m25 443 9 PBMCs PBMCs NNP cord-013178-li1x1m25 443 10 was be VBD cord-013178-li1x1m25 443 11 significantly significantly RB cord-013178-li1x1m25 443 12 lower low JJR cord-013178-li1x1m25 443 13 than than IN cord-013178-li1x1m25 443 14 capsid capsid RB cord-013178-li1x1m25 443 15 - - HYPH cord-013178-li1x1m25 443 16 positive positive JJ cord-013178-li1x1m25 443 17 PBMCs pbmc NNS cord-013178-li1x1m25 443 18 . . . cord-013178-li1x1m25 444 1 Similarly similarly RB cord-013178-li1x1m25 444 2 , , , cord-013178-li1x1m25 444 3 the the DT cord-013178-li1x1m25 444 4 antigen antigen NN cord-013178-li1x1m25 444 5 - - HYPH cord-013178-li1x1m25 444 6 ELISA ELISA NNP cord-013178-li1x1m25 444 7 result result NN cord-013178-li1x1m25 444 8 shows show VBZ cord-013178-li1x1m25 444 9 that that IN cord-013178-li1x1m25 444 10 the the DT cord-013178-li1x1m25 444 11 amount amount NN cord-013178-li1x1m25 444 12 of of IN cord-013178-li1x1m25 444 13 ORF3 ORF3 NNP cord-013178-li1x1m25 444 14 protein protein NN cord-013178-li1x1m25 444 15 was be VBD cord-013178-li1x1m25 444 16 less less JJR cord-013178-li1x1m25 444 17 than than IN cord-013178-li1x1m25 444 18 the the DT cord-013178-li1x1m25 444 19 amount amount NN cord-013178-li1x1m25 444 20 of of IN cord-013178-li1x1m25 444 21 capsid capsid NN cord-013178-li1x1m25 444 22 protein protein NN cord-013178-li1x1m25 444 23 in in IN cord-013178-li1x1m25 444 24 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 444 25 blood blood NN cord-013178-li1x1m25 444 26 from from IN cord-013178-li1x1m25 444 27 suckers sucker NNS cord-013178-li1x1m25 444 28 at at IN cord-013178-li1x1m25 444 29 one one CD cord-013178-li1x1m25 444 30 week week NN cord-013178-li1x1m25 444 31 of of IN cord-013178-li1x1m25 444 32 age age NN cord-013178-li1x1m25 444 33 . . . cord-013178-li1x1m25 445 1 That that DT cord-013178-li1x1m25 445 2 might may MD cord-013178-li1x1m25 445 3 be be VB cord-013178-li1x1m25 445 4 because because IN cord-013178-li1x1m25 445 5 mAb mAb NNP cord-013178-li1x1m25 445 6 7D3 7d3 CD cord-013178-li1x1m25 445 7 only only RB cord-013178-li1x1m25 445 8 recognizes recognize VBZ cord-013178-li1x1m25 445 9 the the DT cord-013178-li1x1m25 445 10 ORF3 ORF3 NNP cord-013178-li1x1m25 445 11 protein protein NN cord-013178-li1x1m25 445 12 ( ( -LRB- cord-013178-li1x1m25 445 13 peptide peptide JJ cord-013178-li1x1m25 445 14 P126 p126 CD cord-013178-li1x1m25 445 15 ) ) -RRB- cord-013178-li1x1m25 445 16 for for IN cord-013178-li1x1m25 445 17 PCV2b pcv2b NN cord-013178-li1x1m25 445 18 strain strain NN cord-013178-li1x1m25 445 19 ( ( -LRB- cord-013178-li1x1m25 445 20 GenBank genbank NN cord-013178-li1x1m25 445 21 : : : cord-013178-li1x1m25 445 22 AAC35332.1 AAC35332.1 NNP cord-013178-li1x1m25 445 23 ) ) -RRB- cord-013178-li1x1m25 445 24 , , , cord-013178-li1x1m25 445 25 and and CC cord-013178-li1x1m25 445 26 it -PRON- PRP cord-013178-li1x1m25 445 27 causes cause VBZ cord-013178-li1x1m25 445 28 low low JJ cord-013178-li1x1m25 445 29 detection detection NN cord-013178-li1x1m25 445 30 . . . cord-013178-li1x1m25 446 1 However however RB cord-013178-li1x1m25 446 2 , , , cord-013178-li1x1m25 446 3 PCV2d PCV2d NNP cord-013178-li1x1m25 446 4 ( ( -LRB- cord-013178-li1x1m25 446 5 PCV2b PCV2b NNP cord-013178-li1x1m25 446 6 mutant mutant JJ cord-013178-li1x1m25 446 7 strain strain NN cord-013178-li1x1m25 446 8 ) ) -RRB- cord-013178-li1x1m25 446 9 and and CC cord-013178-li1x1m25 446 10 PCV2b pcv2b NN cord-013178-li1x1m25 446 11 are be VBP cord-013178-li1x1m25 446 12 still still RB cord-013178-li1x1m25 446 13 predominant predominant JJ cord-013178-li1x1m25 446 14 genotypes genotype NNS cord-013178-li1x1m25 446 15 in in IN cord-013178-li1x1m25 446 16 the the DT cord-013178-li1x1m25 446 17 field field NN cord-013178-li1x1m25 446 18 farms farm NNS cord-013178-li1x1m25 446 19 . . . cord-013178-li1x1m25 447 1 Besides besides IN cord-013178-li1x1m25 447 2 the the DT cord-013178-li1x1m25 447 3 similar similar JJ cord-013178-li1x1m25 447 4 percentages percentage NNS cord-013178-li1x1m25 447 5 of of IN cord-013178-li1x1m25 447 6 PBMCs pbmc NNS cord-013178-li1x1m25 447 7 were be VBD cord-013178-li1x1m25 447 8 positive positive JJ cord-013178-li1x1m25 447 9 for for IN cord-013178-li1x1m25 447 10 these these DT cord-013178-li1x1m25 447 11 antibodies antibody NNS cord-013178-li1x1m25 447 12 ( ( -LRB- cord-013178-li1x1m25 447 13 mAb mAb NNP cord-013178-li1x1m25 447 14 7D3 7d3 CD cord-013178-li1x1m25 447 15 and and CC cord-013178-li1x1m25 447 16 anti anti JJ cord-013178-li1x1m25 447 17 - - JJ cord-013178-li1x1m25 447 18 p53 p53 JJ cord-013178-li1x1m25 447 19 protein protein NN cord-013178-li1x1m25 447 20 rabbit rabbit NN cord-013178-li1x1m25 447 21 polyclonal polyclonal JJ cord-013178-li1x1m25 447 22 antibody antibody NN cord-013178-li1x1m25 447 23 ) ) -RRB- cord-013178-li1x1m25 447 24 staining staining NN cord-013178-li1x1m25 447 25 or or CC cord-013178-li1x1m25 447 26 TUNEL TUNEL NNP cord-013178-li1x1m25 447 27 assay assay NN cord-013178-li1x1m25 447 28 . . . cord-013178-li1x1m25 448 1 In in IN cord-013178-li1x1m25 448 2 contrast contrast NN cord-013178-li1x1m25 448 3 to to IN cord-013178-li1x1m25 448 4 the the DT cord-013178-li1x1m25 448 5 ORF3 ORF3 NNP cord-013178-li1x1m25 448 6 protein protein NN cord-013178-li1x1m25 448 7 , , , cord-013178-li1x1m25 448 8 the the DT cord-013178-li1x1m25 448 9 detection detection NN cord-013178-li1x1m25 448 10 of of IN cord-013178-li1x1m25 448 11 the the DT cord-013178-li1x1m25 448 12 capsid capsid JJ cord-013178-li1x1m25 448 13 protein protein NN cord-013178-li1x1m25 448 14 could could MD cord-013178-li1x1m25 448 15 be be VB cord-013178-li1x1m25 448 16 the the DT cord-013178-li1x1m25 448 17 variable variable JJ cord-013178-li1x1m25 448 18 result result NN cord-013178-li1x1m25 448 19 in in IN cord-013178-li1x1m25 448 20 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 448 21 PBMCs pbmc NNS cord-013178-li1x1m25 448 22 via via IN cord-013178-li1x1m25 448 23 different different JJ cord-013178-li1x1m25 448 24 antibodies antibody NNS cord-013178-li1x1m25 448 25 , , , cord-013178-li1x1m25 448 26 due due IN cord-013178-li1x1m25 448 27 to to IN cord-013178-li1x1m25 448 28 different different JJ cord-013178-li1x1m25 448 29 PCV2 PCV2 NNP cord-013178-li1x1m25 448 30 strains strain NNS cord-013178-li1x1m25 448 31 [ [ -LRB- cord-013178-li1x1m25 448 32 49 49 CD cord-013178-li1x1m25 448 33 , , , cord-013178-li1x1m25 448 34 74 74 CD cord-013178-li1x1m25 448 35 ] ] -RRB- cord-013178-li1x1m25 448 36 , , , cord-013178-li1x1m25 448 37 conformational conformational JJ cord-013178-li1x1m25 448 38 epitopes epitope NNS cord-013178-li1x1m25 448 39 [ [ -LRB- cord-013178-li1x1m25 448 40 75 75 CD cord-013178-li1x1m25 448 41 ] ] -RRB- cord-013178-li1x1m25 448 42 , , , cord-013178-li1x1m25 448 43 or or CC cord-013178-li1x1m25 448 44 linear linear JJ cord-013178-li1x1m25 448 45 epitopes epitope NNS cord-013178-li1x1m25 448 46 [ [ -LRB- cord-013178-li1x1m25 448 47 49 49 CD cord-013178-li1x1m25 448 48 , , , cord-013178-li1x1m25 448 49 76 76 CD cord-013178-li1x1m25 448 50 ] ] -RRB- cord-013178-li1x1m25 448 51 . . . cord-013178-li1x1m25 449 1 Previous previous JJ cord-013178-li1x1m25 449 2 studies study NNS cord-013178-li1x1m25 449 3 indicated indicate VBD cord-013178-li1x1m25 449 4 that that IN cord-013178-li1x1m25 449 5 antibody antibody NN cord-013178-li1x1m25 449 6 binding bind VBG cord-013178-li1x1m25 449 7 residues residue NNS cord-013178-li1x1m25 449 8 were be VBD cord-013178-li1x1m25 449 9 often often RB cord-013178-li1x1m25 449 10 on on IN cord-013178-li1x1m25 449 11 the the DT cord-013178-li1x1m25 449 12 exterior exterior NN cord-013178-li1x1m25 449 13 of of IN cord-013178-li1x1m25 449 14 the the DT cord-013178-li1x1m25 449 15 capsid capsid NN cord-013178-li1x1m25 449 16 of of IN cord-013178-li1x1m25 449 17 PCV2 PCV2 NNP cord-013178-li1x1m25 449 18 [ [ -LRB- cord-013178-li1x1m25 449 19 48 48 CD cord-013178-li1x1m25 449 20 , , , cord-013178-li1x1m25 449 21 49 49 CD cord-013178-li1x1m25 449 22 , , , cord-013178-li1x1m25 449 23 70 70 CD cord-013178-li1x1m25 449 24 , , , cord-013178-li1x1m25 449 25 74 74 CD cord-013178-li1x1m25 449 26 , , , cord-013178-li1x1m25 449 27 77 77 CD cord-013178-li1x1m25 449 28 , , , cord-013178-li1x1m25 449 29 78 78 CD cord-013178-li1x1m25 449 30 ] ] -RRB- cord-013178-li1x1m25 449 31 . . . cord-013178-li1x1m25 450 1 Although although IN cord-013178-li1x1m25 450 2 an an DT cord-013178-li1x1m25 450 3 epitope epitope NN cord-013178-li1x1m25 450 4 might may MD cord-013178-li1x1m25 450 5 be be VB cord-013178-li1x1m25 450 6 on on IN cord-013178-li1x1m25 450 7 the the DT cord-013178-li1x1m25 450 8 surface surface NN cord-013178-li1x1m25 450 9 of of IN cord-013178-li1x1m25 450 10 the the DT cord-013178-li1x1m25 450 11 single single JJ cord-013178-li1x1m25 450 12 capsid capsid JJ cord-013178-li1x1m25 450 13 unit unit NN cord-013178-li1x1m25 450 14 , , , cord-013178-li1x1m25 450 15 it -PRON- PRP cord-013178-li1x1m25 450 16 could could MD cord-013178-li1x1m25 450 17 bury bury VB cord-013178-li1x1m25 450 18 in in IN cord-013178-li1x1m25 450 19 the the DT cord-013178-li1x1m25 450 20 VLP VLP NNP cord-013178-li1x1m25 450 21 and and CC cord-013178-li1x1m25 450 22 be be VB cord-013178-li1x1m25 450 23 inaccessible inaccessible JJ cord-013178-li1x1m25 450 24 to to IN cord-013178-li1x1m25 450 25 the the DT cord-013178-li1x1m25 450 26 antibody antibody NN cord-013178-li1x1m25 450 27 [ [ -LRB- cord-013178-li1x1m25 450 28 79 79 CD cord-013178-li1x1m25 450 29 , , , cord-013178-li1x1m25 450 30 80 80 CD cord-013178-li1x1m25 450 31 ] ] -RRB- cord-013178-li1x1m25 450 32 . . . cord-013178-li1x1m25 451 1 In in IN cord-013178-li1x1m25 451 2 general general JJ cord-013178-li1x1m25 451 3 , , , cord-013178-li1x1m25 451 4 these these DT cord-013178-li1x1m25 451 5 results result NNS cord-013178-li1x1m25 451 6 confirm confirm VBP cord-013178-li1x1m25 451 7 that that IN cord-013178-li1x1m25 451 8 mAb mAb NNP cord-013178-li1x1m25 451 9 7D3 7D3 NNP cord-013178-li1x1m25 451 10 recognized recognize VBD cord-013178-li1x1m25 451 11 the the DT cord-013178-li1x1m25 451 12 native native JJ cord-013178-li1x1m25 451 13 ORF3 ORF3 NNP cord-013178-li1x1m25 451 14 protein protein NN cord-013178-li1x1m25 451 15 in in IN cord-013178-li1x1m25 451 16 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 451 17 PBMCs pbmc NNS cord-013178-li1x1m25 451 18 . . . cord-013178-li1x1m25 452 1 This this DT cord-013178-li1x1m25 452 2 study study NN cord-013178-li1x1m25 452 3 used use VBD cord-013178-li1x1m25 452 4 mAb mAb NNP cord-013178-li1x1m25 452 5 7D3 7d3 CD cord-013178-li1x1m25 452 6 or or CC cord-013178-li1x1m25 452 7 its -PRON- PRP$ cord-013178-li1x1m25 452 8 minimal minimal JJ cord-013178-li1x1m25 452 9 linear linear JJ cord-013178-li1x1m25 452 10 epitope epitope NN cord-013178-li1x1m25 452 11 to to TO cord-013178-li1x1m25 452 12 design design VB cord-013178-li1x1m25 452 13 various various JJ cord-013178-li1x1m25 452 14 immunoassays immunoassay NNS cord-013178-li1x1m25 452 15 . . . cord-013178-li1x1m25 453 1 These these DT cord-013178-li1x1m25 453 2 assays assay NNS cord-013178-li1x1m25 453 3 evaluate evaluate VBP cord-013178-li1x1m25 453 4 the the DT cord-013178-li1x1m25 453 5 viral viral JJ cord-013178-li1x1m25 453 6 load load NN cord-013178-li1x1m25 453 7 and and CC cord-013178-li1x1m25 453 8 the the DT cord-013178-li1x1m25 453 9 immune immune JJ cord-013178-li1x1m25 453 10 response response NN cord-013178-li1x1m25 453 11 of of IN cord-013178-li1x1m25 453 12 viral viral JJ cord-013178-li1x1m25 453 13 proteins protein NNS cord-013178-li1x1m25 453 14 stimuli stimulus NNS cord-013178-li1x1m25 453 15 . . . cord-013178-li1x1m25 454 1 These these DT cord-013178-li1x1m25 454 2 may may MD cord-013178-li1x1m25 454 3 serve serve VB cord-013178-li1x1m25 454 4 as as IN cord-013178-li1x1m25 454 5 surveillance surveillance NN cord-013178-li1x1m25 454 6 tools tool NNS cord-013178-li1x1m25 454 7 for for IN cord-013178-li1x1m25 454 8 monitoring monitor VBG cord-013178-li1x1m25 454 9 natural natural JJ cord-013178-li1x1m25 454 10 PCV2 PCV2 NNP cord-013178-li1x1m25 454 11 infection infection NN cord-013178-li1x1m25 454 12 in in IN cord-013178-li1x1m25 454 13 the the DT cord-013178-li1x1m25 454 14 herd herd NN cord-013178-li1x1m25 454 15 . . . cord-013178-li1x1m25 455 1 Overall overall RB cord-013178-li1x1m25 455 2 , , , cord-013178-li1x1m25 455 3 the the DT cord-013178-li1x1m25 455 4 author author NN cord-013178-li1x1m25 455 5 generated generate VBD cord-013178-li1x1m25 455 6 anti anti NNP cord-013178-li1x1m25 455 7 - - NNP cord-013178-li1x1m25 455 8 ORF3 ORF3 NNP cord-013178-li1x1m25 455 9 mAbs mAbs NNP cord-013178-li1x1m25 455 10 against against IN cord-013178-li1x1m25 455 11 the the DT cord-013178-li1x1m25 455 12 ORF3 ORF3 NNP cord-013178-li1x1m25 455 13 peptide peptide NN cord-013178-li1x1m25 455 14 ( ( -LRB- cord-013178-li1x1m25 455 15 residues residue NNS cord-013178-li1x1m25 455 16 36 36 CD cord-013178-li1x1m25 455 17 - - SYM cord-013178-li1x1m25 455 18 66 66 CD cord-013178-li1x1m25 455 19 ) ) -RRB- cord-013178-li1x1m25 455 20 of of IN cord-013178-li1x1m25 455 21 PCV2 PCV2 NNP cord-013178-li1x1m25 455 22 . . . cord-013178-li1x1m25 456 1 This this DT cord-013178-li1x1m25 456 2 study study NN cord-013178-li1x1m25 456 3 confirmed confirm VBD cord-013178-li1x1m25 456 4 the the DT cord-013178-li1x1m25 456 5 defective defective JJ cord-013178-li1x1m25 456 6 - - HYPH cord-013178-li1x1m25 456 7 Ig ig NN cord-013178-li1x1m25 456 8 mAb mab NN cord-013178-li1x1m25 456 9 ( ( -LRB- cord-013178-li1x1m25 456 10 6D10 6d10 CD cord-013178-li1x1m25 456 11 ) ) -RRB- cord-013178-li1x1m25 456 12 with with IN cord-013178-li1x1m25 456 13 a a DT cord-013178-li1x1m25 456 14 lambda lambda NN cord-013178-li1x1m25 456 15 - - HYPH cord-013178-li1x1m25 456 16 light light JJ cord-013178-li1x1m25 456 17 chain chain NN cord-013178-li1x1m25 456 18 , , , cord-013178-li1x1m25 456 19 and and CC cord-013178-li1x1m25 456 20 its -PRON- PRP$ cord-013178-li1x1m25 456 21 molecular molecular JJ cord-013178-li1x1m25 456 22 weight weight NN cord-013178-li1x1m25 456 23 was be VBD cord-013178-li1x1m25 456 24 about about RB cord-013178-li1x1m25 456 25 33 33 CD cord-013178-li1x1m25 456 26 kDa kDa NNS cord-013178-li1x1m25 456 27 . . . cord-013178-li1x1m25 457 1 The the DT cord-013178-li1x1m25 457 2 mAb mAb NNP cord-013178-li1x1m25 457 3 7D3 7D3 NNP cord-013178-li1x1m25 457 4 contained contain VBD cord-013178-li1x1m25 457 5 heavy heavy JJ cord-013178-li1x1m25 457 6 chains chain NNS cord-013178-li1x1m25 457 7 ( ( -LRB- cord-013178-li1x1m25 457 8 γ γ NN cord-013178-li1x1m25 457 9 1 1 CD cord-013178-li1x1m25 457 10 ) ) -RRB- cord-013178-li1x1m25 457 11 and and CC cord-013178-li1x1m25 457 12 kappa kappa NN cord-013178-li1x1m25 457 13 - - HYPH cord-013178-li1x1m25 457 14 light light JJ cord-013178-li1x1m25 457 15 chains chain NNS cord-013178-li1x1m25 457 16 , , , cord-013178-li1x1m25 457 17 and and CC cord-013178-li1x1m25 457 18 it -PRON- PRP cord-013178-li1x1m25 457 19 bound bind VBD cord-013178-li1x1m25 457 20 to to IN cord-013178-li1x1m25 457 21 one one CD cord-013178-li1x1m25 457 22 minimal minimal JJ cord-013178-li1x1m25 457 23 linear linear JJ cord-013178-li1x1m25 457 24 epitope epitope NN cord-013178-li1x1m25 457 25 ( ( -LRB- cord-013178-li1x1m25 457 26 HNDVYISLPITLLHFPAHFQ HNDVYISLPITLLHFPAHFQ NNP cord-013178-li1x1m25 457 27 KFSQPAEISDKR KFSQPAEISDKR NNP cord-013178-li1x1m25 457 28 ) ) -RRB- cord-013178-li1x1m25 457 29 . . . cord-013178-li1x1m25 458 1 The the DT cord-013178-li1x1m25 458 2 data datum NNS cord-013178-li1x1m25 458 3 show show VBP cord-013178-li1x1m25 458 4 that that IN cord-013178-li1x1m25 458 5 3 3 CD cord-013178-li1x1m25 458 6 - - SYM cord-013178-li1x1m25 458 7 5 5 CD cord-013178-li1x1m25 458 8 % % NN cord-013178-li1x1m25 458 9 of of IN cord-013178-li1x1m25 458 10 PBMCs pbmc NNS cord-013178-li1x1m25 458 11 were be VBD cord-013178-li1x1m25 458 12 positive positive JJ cord-013178-li1x1m25 458 13 for for IN cord-013178-li1x1m25 458 14 ORF3 ORF3 NNP cord-013178-li1x1m25 458 15 protein protein NN cord-013178-li1x1m25 458 16 or or CC cord-013178-li1x1m25 458 17 p53 p53 NN cord-013178-li1x1m25 458 18 protein protein NN cord-013178-li1x1m25 458 19 . . . cord-013178-li1x1m25 459 1 Otherwise otherwise RB cord-013178-li1x1m25 459 2 , , , cord-013178-li1x1m25 459 3 78 78 CD cord-013178-li1x1m25 459 4 - - SYM cord-013178-li1x1m25 459 5 82 82 CD cord-013178-li1x1m25 459 6 % % NN cord-013178-li1x1m25 459 7 of of IN cord-013178-li1x1m25 459 8 PBMCs pbmc NNS cord-013178-li1x1m25 459 9 were be VBD cord-013178-li1x1m25 459 10 positive positive JJ cord-013178-li1x1m25 459 11 for for IN cord-013178-li1x1m25 459 12 anti anti JJ cord-013178-li1x1m25 459 13 - - JJ cord-013178-li1x1m25 459 14 capsid capsid JJ cord-013178-li1x1m25 459 15 peptide peptide NN cord-013178-li1x1m25 459 16 mAb mAb NNS cord-013178-li1x1m25 459 17 ( ( -LRB- cord-013178-li1x1m25 459 18 1H3 1h3 CD cord-013178-li1x1m25 459 19 ) ) -RRB- cord-013178-li1x1m25 459 20 staining staining NN cord-013178-li1x1m25 459 21 . . . cord-013178-li1x1m25 460 1 This this DT cord-013178-li1x1m25 460 2 study study NN cord-013178-li1x1m25 460 3 confirmed confirm VBD cord-013178-li1x1m25 460 4 the the DT cord-013178-li1x1m25 460 5 ORF3 ORF3 NNP cord-013178-li1x1m25 460 6 protein protein NN cord-013178-li1x1m25 460 7 colocalized colocalize VBN cord-013178-li1x1m25 460 8 with with IN cord-013178-li1x1m25 460 9 the the DT cord-013178-li1x1m25 460 10 p53 p53 NN cord-013178-li1x1m25 460 11 protein protein NN cord-013178-li1x1m25 460 12 in in IN cord-013178-li1x1m25 460 13 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 460 14 PBMCs pbmc NNS cord-013178-li1x1m25 460 15 . . . cord-013178-li1x1m25 461 1 The the DT cord-013178-li1x1m25 461 2 author author NN cord-013178-li1x1m25 461 3 devised devise VBD cord-013178-li1x1m25 461 4 the the DT cord-013178-li1x1m25 461 5 ORF3 ORF3 NNP cord-013178-li1x1m25 461 6 protein protein NN cord-013178-li1x1m25 461 7 - - HYPH cord-013178-li1x1m25 461 8 ELISA ELISA NNP cord-013178-li1x1m25 461 9 ( ( -LRB- cord-013178-li1x1m25 461 10 anti anti NNP cord-013178-li1x1m25 461 11 - - JJ cord-013178-li1x1m25 461 12 ORF3 orf3 JJ cord-013178-li1x1m25 461 13 antisera antisera NN cord-013178-li1x1m25 461 14 and and CC cord-013178-li1x1m25 461 15 mAb mAb NNPS cord-013178-li1x1m25 461 16 7D3-based 7d3-based CD cord-013178-li1x1m25 461 17 ) ) -RRB- cord-013178-li1x1m25 461 18 to to TO cord-013178-li1x1m25 461 19 detect detect VB cord-013178-li1x1m25 461 20 the the DT cord-013178-li1x1m25 461 21 ORF3 ORF3 NNP cord-013178-li1x1m25 461 22 protein protein NN cord-013178-li1x1m25 461 23 in in IN cord-013178-li1x1m25 461 24 blood blood NN cord-013178-li1x1m25 461 25 samples sample NNS cord-013178-li1x1m25 461 26 . . . cord-013178-li1x1m25 462 1 The the DT cord-013178-li1x1m25 462 2 results result NNS cord-013178-li1x1m25 462 3 show show VBP cord-013178-li1x1m25 462 4 that that IN cord-013178-li1x1m25 462 5 the the DT cord-013178-li1x1m25 462 6 amount amount NN cord-013178-li1x1m25 462 7 of of IN cord-013178-li1x1m25 462 8 ORF3 ORF3 NNP cord-013178-li1x1m25 462 9 protein protein NN cord-013178-li1x1m25 462 10 was be VBD cord-013178-li1x1m25 462 11 less less JJR cord-013178-li1x1m25 462 12 than than IN cord-013178-li1x1m25 462 13 the the DT cord-013178-li1x1m25 462 14 amount amount NN cord-013178-li1x1m25 462 15 of of IN cord-013178-li1x1m25 462 16 capsid capsid NN cord-013178-li1x1m25 462 17 protein protein NN cord-013178-li1x1m25 462 18 in in IN cord-013178-li1x1m25 462 19 PCV2-infected pcv2-infected JJ cord-013178-li1x1m25 462 20 blood blood NN cord-013178-li1x1m25 462 21 from from IN cord-013178-li1x1m25 462 22 suckers sucker NNS cord-013178-li1x1m25 462 23 at at IN cord-013178-li1x1m25 462 24 one one CD cord-013178-li1x1m25 462 25 week week NN cord-013178-li1x1m25 462 26 of of IN cord-013178-li1x1m25 462 27 age age NN cord-013178-li1x1m25 462 28 . . . cord-013178-li1x1m25 463 1 The the DT cord-013178-li1x1m25 463 2 correlation correlation NN cord-013178-li1x1m25 463 3 between between IN cord-013178-li1x1m25 463 4 the the DT cord-013178-li1x1m25 463 5 ORF3 ORF3 NNP cord-013178-li1x1m25 463 6 protein protein NN cord-013178-li1x1m25 463 7 and and CC cord-013178-li1x1m25 463 8 the the DT cord-013178-li1x1m25 463 9 capsid capsid JJ cord-013178-li1x1m25 463 10 protein protein NN cord-013178-li1x1m25 463 11 is be VBZ cord-013178-li1x1m25 463 12 worth worth JJ cord-013178-li1x1m25 463 13 noting note VBG cord-013178-li1x1m25 463 14 ( ( -LRB- cord-013178-li1x1m25 463 15 r r NN cord-013178-li1x1m25 463 16 = = SYM cord-013178-li1x1m25 463 17 0.7583 0.7583 CD cord-013178-li1x1m25 463 18 ) ) -RRB- cord-013178-li1x1m25 463 19 . . . cord-013178-li1x1m25 464 1 Furthermore furthermore RB cord-013178-li1x1m25 464 2 , , , cord-013178-li1x1m25 464 3 the the DT cord-013178-li1x1m25 464 4 antibody antibody NN cord-013178-li1x1m25 464 5 level level NN cord-013178-li1x1m25 464 6 of of IN cord-013178-li1x1m25 464 7 anti anti JJ cord-013178-li1x1m25 464 8 - - JJ cord-013178-li1x1m25 464 9 capsid capsid JJ cord-013178-li1x1m25 464 10 IgG igg NN cord-013178-li1x1m25 464 11 and and CC cord-013178-li1x1m25 464 12 anti anti NNP cord-013178-li1x1m25 464 13 - - NNP cord-013178-li1x1m25 464 14 ORF3 orf3 JJ cord-013178-li1x1m25 464 15 IgG igg NN cord-013178-li1x1m25 464 16 could could MD cord-013178-li1x1m25 464 17 imply imply VB cord-013178-li1x1m25 464 18 the the DT cord-013178-li1x1m25 464 19 immunity immunity NN cord-013178-li1x1m25 464 20 response response NN cord-013178-li1x1m25 464 21 of of IN cord-013178-li1x1m25 464 22 pig pig NN cord-013178-li1x1m25 464 23 herd herd NN cord-013178-li1x1m25 464 24 , , , cord-013178-li1x1m25 464 25 but but CC cord-013178-li1x1m25 464 26 the the DT cord-013178-li1x1m25 464 27 sample sample NN cord-013178-li1x1m25 464 28 size size NN cord-013178-li1x1m25 464 29 needs need VBZ cord-013178-li1x1m25 464 30 to to TO cord-013178-li1x1m25 464 31 be be VB cord-013178-li1x1m25 464 32 considered consider VBN cord-013178-li1x1m25 464 33 . . . cord-013178-li1x1m25 464 34 _SP cord-013178-li1x1m25 465 1 The the DT cord-013178-li1x1m25 465 2 author author NN cord-013178-li1x1m25 465 3 declares declare VBZ cord-013178-li1x1m25 465 4 no no DT cord-013178-li1x1m25 465 5 conflict conflict NN cord-013178-li1x1m25 465 6 of of IN cord-013178-li1x1m25 465 7 interest interest NN cord-013178-li1x1m25 465 8 . . . cord-013178-li1x1m25 465 9 _SP cord-013178-li1x1m25 466 1 A a DT cord-013178-li1x1m25 466 2 very very RB cord-013178-li1x1m25 466 3 small small JJ cord-013178-li1x1m25 466 4 porcine porcine JJ cord-013178-li1x1m25 466 5 virus virus NN cord-013178-li1x1m25 466 6 with with IN cord-013178-li1x1m25 466 7 circular circular JJ cord-013178-li1x1m25 466 8 single single RB cord-013178-li1x1m25 466 9 - - HYPH cord-013178-li1x1m25 466 10 stranded strand VBN cord-013178-li1x1m25 466 11 DNA dna NN cord-013178-li1x1m25 466 12 Characterization characterization NN cord-013178-li1x1m25 466 13 of of IN cord-013178-li1x1m25 466 14 novel novel JJ cord-013178-li1x1m25 466 15 circovirus circovirus NNP cord-013178-li1x1m25 466 16 DNAs dna NNS cord-013178-li1x1m25 466 17 associated associate VBN cord-013178-li1x1m25 466 18 with with IN cord-013178-li1x1m25 466 19 wasting waste VBG cord-013178-li1x1m25 466 20 syndromes syndrome NNS cord-013178-li1x1m25 466 21 in in IN cord-013178-li1x1m25 466 22 pigs pig NNS cord-013178-li1x1m25 466 23 Studies Studies NNPS cord-013178-li1x1m25 466 24 on on IN cord-013178-li1x1m25 466 25 epidemiology epidemiology NN cord-013178-li1x1m25 466 26 and and CC cord-013178-li1x1m25 466 27 pathogenicity pathogenicity NN cord-013178-li1x1m25 466 28 of of IN cord-013178-li1x1m25 466 29 porcine porcine JJ cord-013178-li1x1m25 466 30 circovirus circovirus JJ cord-013178-li1x1m25 466 31 Experimental experimental JJ cord-013178-li1x1m25 466 32 reproduction reproduction NN cord-013178-li1x1m25 466 33 of of IN cord-013178-li1x1m25 466 34 severe severe JJ cord-013178-li1x1m25 466 35 wasting waste VBG cord-013178-li1x1m25 466 36 disease disease NN cord-013178-li1x1m25 466 37 by by IN cord-013178-li1x1m25 466 38 co co NN cord-013178-li1x1m25 466 39 - - NN cord-013178-li1x1m25 466 40 infection infection NN cord-013178-li1x1m25 466 41 of of IN cord-013178-li1x1m25 466 42 pigs pig NNS cord-013178-li1x1m25 466 43 with with IN cord-013178-li1x1m25 466 44 porcine porcine JJ cord-013178-li1x1m25 466 45 circovirus circovirus JJ cord-013178-li1x1m25 466 46 and and CC cord-013178-li1x1m25 466 47 porcine porcine JJ cord-013178-li1x1m25 466 48 parvovirus parvovirus NN cord-013178-li1x1m25 466 49 Reproduction reproduction NN cord-013178-li1x1m25 466 50 of of IN cord-013178-li1x1m25 466 51 lesions lesion NNS cord-013178-li1x1m25 466 52 of of IN cord-013178-li1x1m25 466 53 postweaning postweane VBG cord-013178-li1x1m25 466 54 multisystemic multisystemic NNP cord-013178-li1x1m25 466 55 wasting waste VBG cord-013178-li1x1m25 466 56 syndrome syndrome NN cord-013178-li1x1m25 466 57 by by IN cord-013178-li1x1m25 466 58 infection infection NN cord-013178-li1x1m25 466 59 of of IN cord-013178-li1x1m25 466 60 conventional conventional JJ cord-013178-li1x1m25 466 61 pigs pig NNS cord-013178-li1x1m25 466 62 with with IN cord-013178-li1x1m25 466 63 porcine porcine JJ cord-013178-li1x1m25 466 64 circovirus circovirus JJ cord-013178-li1x1m25 466 65 type type NN cord-013178-li1x1m25 466 66 2 2 CD cord-013178-li1x1m25 466 67 alone alone RB cord-013178-li1x1m25 466 68 or or CC cord-013178-li1x1m25 466 69 in in IN cord-013178-li1x1m25 466 70 combination combination NN cord-013178-li1x1m25 466 71 with with IN cord-013178-li1x1m25 466 72 porcine porcine JJ cord-013178-li1x1m25 466 73 parvovirus parvovirus NN cord-013178-li1x1m25 466 74 Enteritis enteritis NN cord-013178-li1x1m25 466 75 associated associate VBN cord-013178-li1x1m25 466 76 with with IN cord-013178-li1x1m25 466 77 porcine porcine JJ cord-013178-li1x1m25 466 78 circovirus circovirus NNP cord-013178-li1x1m25 466 79 2 2 CD cord-013178-li1x1m25 466 80 in in IN cord-013178-li1x1m25 466 81 pigs pig NNS cord-013178-li1x1m25 466 82 Porcine porcine JJ cord-013178-li1x1m25 466 83 circovirus circovirus JJ cord-013178-li1x1m25 466 84 type type NN cord-013178-li1x1m25 466 85 2 2 CD cord-013178-li1x1m25 466 86 associated associate VBD cord-013178-li1x1m25 466 87 disease disease NN cord-013178-li1x1m25 466 88 : : : cord-013178-li1x1m25 466 89 Update update VB cord-013178-li1x1m25 466 90 on on IN cord-013178-li1x1m25 466 91 current current JJ cord-013178-li1x1m25 466 92 terminology terminology NN cord-013178-li1x1m25 466 93 , , , cord-013178-li1x1m25 466 94 clinical clinical JJ cord-013178-li1x1m25 466 95 manifestations manifestation NNS cord-013178-li1x1m25 466 96 , , , cord-013178-li1x1m25 466 97 pathogenesis pathogenesis NN cord-013178-li1x1m25 466 98 , , , cord-013178-li1x1m25 466 99 diagnosis diagnosis NN cord-013178-li1x1m25 466 100 , , , cord-013178-li1x1m25 466 101 and and CC cord-013178-li1x1m25 466 102 intervention intervention NN cord-013178-li1x1m25 466 103 strategies strategy NNS cord-013178-li1x1m25 467 1 Porcine porcine JJ cord-013178-li1x1m25 467 2 circovirus circovirus JJ cord-013178-li1x1m25 467 3 type type NN cord-013178-li1x1m25 467 4 2 2 CD cord-013178-li1x1m25 467 5 ( ( -LRB- cord-013178-li1x1m25 467 6 PCV2 PCV2 NNP cord-013178-li1x1m25 467 7 ) ) -RRB- cord-013178-li1x1m25 467 8 infections infection NNS cord-013178-li1x1m25 467 9 : : : cord-013178-li1x1m25 468 1 Clinical clinical JJ cord-013178-li1x1m25 468 2 signs sign NNS cord-013178-li1x1m25 468 3 , , , cord-013178-li1x1m25 468 4 pathology pathology NN cord-013178-li1x1m25 468 5 and and CC cord-013178-li1x1m25 468 6 laboratory laboratory NN cord-013178-li1x1m25 468 7 diagnosis diagnosis NN cord-013178-li1x1m25 468 8 Porcine Porcine NNP cord-013178-li1x1m25 468 9 circovirus circovirus NNP cord-013178-li1x1m25 468 10 induces induce VBZ cord-013178-li1x1m25 468 11 B b NN cord-013178-li1x1m25 468 12 lymphocyte lymphocyte NN cord-013178-li1x1m25 468 13 depletion depletion NN cord-013178-li1x1m25 468 14 in in IN cord-013178-li1x1m25 468 15 pigs pig NNS cord-013178-li1x1m25 468 16 with with IN cord-013178-li1x1m25 468 17 wasting waste VBG cord-013178-li1x1m25 468 18 disease disease NN cord-013178-li1x1m25 468 19 syndrome syndrome NN cord-013178-li1x1m25 468 20 Postweaning Postweaning NNP cord-013178-li1x1m25 468 21 mulstisystemic mulstisystemic NNP cord-013178-li1x1m25 468 22 wasting waste VBG cord-013178-li1x1m25 468 23 syndrome syndrome NN cord-013178-li1x1m25 468 24 ( ( -LRB- cord-013178-li1x1m25 468 25 PMWS PMWS NNP cord-013178-li1x1m25 468 26 ) ) -RRB- cord-013178-li1x1m25 468 27 in in IN cord-013178-li1x1m25 468 28 pigs pig NNS cord-013178-li1x1m25 468 29 . . . cord-013178-li1x1m25 469 1 A a DT cord-013178-li1x1m25 469 2 review review NN cord-013178-li1x1m25 469 3 Changes change NNS cord-013178-li1x1m25 469 4 in in IN cord-013178-li1x1m25 469 5 peripheral peripheral JJ cord-013178-li1x1m25 469 6 blood blood NN cord-013178-li1x1m25 469 7 leukocyte leukocyte NN cord-013178-li1x1m25 469 8 populations population NNS cord-013178-li1x1m25 469 9 in in IN cord-013178-li1x1m25 469 10 pigs pig NNS cord-013178-li1x1m25 469 11 with with IN cord-013178-li1x1m25 469 12 natural natural JJ cord-013178-li1x1m25 469 13 postweaning postweaning NNP cord-013178-li1x1m25 469 14 multisystemic multisystemic NNP cord-013178-li1x1m25 469 15 wasting waste VBG cord-013178-li1x1m25 469 16 syndrome syndrome NN cord-013178-li1x1m25 469 17 ( ( -LRB- cord-013178-li1x1m25 469 18 PMWS PMWS NNP cord-013178-li1x1m25 469 19 ) ) -RRB- cord-013178-li1x1m25 470 1 Can Can MD cord-013178-li1x1m25 470 2 Porcine porcine JJ cord-013178-li1x1m25 470 3 circovirus circovirus JJ cord-013178-li1x1m25 470 4 type type NN cord-013178-li1x1m25 470 5 2 2 CD cord-013178-li1x1m25 471 1 ( ( -LRB- cord-013178-li1x1m25 471 2 PCV2 pcv2 JJ cord-013178-li1x1m25 471 3 ) ) -RRB- cord-013178-li1x1m25 471 4 infection infection NN cord-013178-li1x1m25 471 5 be be VB cord-013178-li1x1m25 471 6 eradicated eradicate VBN cord-013178-li1x1m25 471 7 by by IN cord-013178-li1x1m25 471 8 mass mass NN cord-013178-li1x1m25 471 9 vaccination vaccination NN cord-013178-li1x1m25 471 10 ? ? . cord-013178-li1x1m25 472 1 Comparative comparative JJ cord-013178-li1x1m25 472 2 efficacy efficacy NN cord-013178-li1x1m25 472 3 of of IN cord-013178-li1x1m25 472 4 three three CD cord-013178-li1x1m25 472 5 commercial commercial JJ cord-013178-li1x1m25 472 6 PCV2 PCV2 NNP cord-013178-li1x1m25 472 7 vaccines vaccine NNS cord-013178-li1x1m25 472 8 in in IN cord-013178-li1x1m25 472 9 conventionally conventionally RB cord-013178-li1x1m25 472 10 reared rear VBN cord-013178-li1x1m25 472 11 pigs pig NNS cord-013178-li1x1m25 472 12 Efficacy Efficacy NNP cord-013178-li1x1m25 472 13 of of IN cord-013178-li1x1m25 472 14 the the DT cord-013178-li1x1m25 472 15 Porcine porcine JJ cord-013178-li1x1m25 472 16 circovirus circovirus NNP cord-013178-li1x1m25 472 17 2 2 CD cord-013178-li1x1m25 472 18 ( ( -LRB- cord-013178-li1x1m25 472 19 PCV2 PCV2 NNP cord-013178-li1x1m25 472 20 ) ) -RRB- cord-013178-li1x1m25 472 21 vaccination vaccination NN cord-013178-li1x1m25 472 22 under under IN cord-013178-li1x1m25 472 23 field field NN cord-013178-li1x1m25 472 24 conditions condition NNS cord-013178-li1x1m25 472 25 Evaluation Evaluation NNP cord-013178-li1x1m25 472 26 of of IN cord-013178-li1x1m25 472 27 natural natural JJ cord-013178-li1x1m25 472 28 porcine porcine JJ cord-013178-li1x1m25 472 29 circovirus circovirus NN cord-013178-li1x1m25 472 30 type type NN cord-013178-li1x1m25 472 31 2 2 CD cord-013178-li1x1m25 472 32 ( ( -LRB- cord-013178-li1x1m25 472 33 PCV2 pcv2 JJ cord-013178-li1x1m25 472 34 ) ) -RRB- cord-013178-li1x1m25 472 35 subclinical subclinical JJ cord-013178-li1x1m25 472 36 infection infection NN cord-013178-li1x1m25 472 37 and and CC cord-013178-li1x1m25 472 38 seroconversion seroconversion NN cord-013178-li1x1m25 472 39 dynamics dynamic NNS cord-013178-li1x1m25 472 40 in in IN cord-013178-li1x1m25 472 41 piglets piglet NNS cord-013178-li1x1m25 472 42 vaccinated vaccinate VBN cord-013178-li1x1m25 472 43 at at IN cord-013178-li1x1m25 472 44 different different JJ cord-013178-li1x1m25 472 45 ages age NNS cord-013178-li1x1m25 473 1 A a DT cord-013178-li1x1m25 473 2 new new JJ cord-013178-li1x1m25 473 3 emerging emerge VBG cord-013178-li1x1m25 473 4 genotype genotype NN cord-013178-li1x1m25 473 5 subgroup subgroup NN cord-013178-li1x1m25 473 6 within within IN cord-013178-li1x1m25 473 7 PCV-2b PCV-2b NNP cord-013178-li1x1m25 473 8 dominates dominate VBZ cord-013178-li1x1m25 473 9 the the DT cord-013178-li1x1m25 473 10 PMWS PMWS NNP cord-013178-li1x1m25 473 11 epizooty epizooty NNS cord-013178-li1x1m25 473 12 in in IN cord-013178-li1x1m25 473 13 Switzerland Switzerland NNP cord-013178-li1x1m25 473 14 Epidemiology Epidemiology NNP cord-013178-li1x1m25 473 15 and and CC cord-013178-li1x1m25 473 16 horizontal horizontal JJ cord-013178-li1x1m25 473 17 transmission transmission NN cord-013178-li1x1m25 473 18 of of IN cord-013178-li1x1m25 473 19 porcine porcine JJ cord-013178-li1x1m25 473 20 circovirus circovirus JJ cord-013178-li1x1m25 473 21 type type NN cord-013178-li1x1m25 473 22 2 2 CD cord-013178-li1x1m25 474 1 ( ( -LRB- cord-013178-li1x1m25 474 2 PCV2 PCV2 NNP cord-013178-li1x1m25 474 3 ) ) -RRB- cord-013178-li1x1m25 474 4 Genotypic genotypic JJ cord-013178-li1x1m25 474 5 shift shift NN cord-013178-li1x1m25 474 6 of of IN cord-013178-li1x1m25 474 7 porcine porcine JJ cord-013178-li1x1m25 474 8 circovirus circovirus JJ cord-013178-li1x1m25 474 9 type type NN cord-013178-li1x1m25 474 10 2 2 CD cord-013178-li1x1m25 474 11 from from IN cord-013178-li1x1m25 474 12 PCV-2a PCV-2a NNP cord-013178-li1x1m25 474 13 to to IN cord-013178-li1x1m25 474 14 PCV-2b PCV-2b NNP cord-013178-li1x1m25 474 15 in in IN cord-013178-li1x1m25 474 16 Spain Spain NNP cord-013178-li1x1m25 474 17 from from IN cord-013178-li1x1m25 474 18 Efficacy Efficacy NNP cord-013178-li1x1m25 474 19 and and CC cord-013178-li1x1m25 474 20 future future JJ cord-013178-li1x1m25 474 21 prospects prospect NNS cord-013178-li1x1m25 474 22 of of IN cord-013178-li1x1m25 474 23 commercially commercially RB cord-013178-li1x1m25 474 24 available available JJ cord-013178-li1x1m25 474 25 and and CC cord-013178-li1x1m25 474 26 experimental experimental JJ cord-013178-li1x1m25 474 27 vaccines vaccine NNS cord-013178-li1x1m25 474 28 against against IN cord-013178-li1x1m25 474 29 porcine porcine JJ cord-013178-li1x1m25 474 30 circovirus circovirus JJ cord-013178-li1x1m25 474 31 type type NN cord-013178-li1x1m25 474 32 2 2 CD cord-013178-li1x1m25 475 1 ( ( -LRB- cord-013178-li1x1m25 475 2 PCV2 PCV2 NNP cord-013178-li1x1m25 475 3 ) ) -RRB- cord-013178-li1x1m25 476 1 Genetic genetic JJ cord-013178-li1x1m25 476 2 variation variation NN cord-013178-li1x1m25 476 3 analysis analysis NN cord-013178-li1x1m25 476 4 of of IN cord-013178-li1x1m25 476 5 Chinese chinese JJ cord-013178-li1x1m25 476 6 strains strain NNS cord-013178-li1x1m25 476 7 of of IN cord-013178-li1x1m25 476 8 porcine porcine JJ cord-013178-li1x1m25 476 9 circovirus circovirus JJ cord-013178-li1x1m25 476 10 type type NN cord-013178-li1x1m25 476 11 2 2 CD cord-013178-li1x1m25 476 12 Porcine porcine JJ cord-013178-li1x1m25 476 13 circovirus circovirus JJ cord-013178-li1x1m25 476 14 type type NN cord-013178-li1x1m25 476 15 2 2 CD cord-013178-li1x1m25 477 1 ( ( -LRB- cord-013178-li1x1m25 477 2 PCV2 PCV2 NNP cord-013178-li1x1m25 477 3 ) ) -RRB- cord-013178-li1x1m25 477 4 : : : cord-013178-li1x1m25 478 1 Genetic genetic JJ cord-013178-li1x1m25 478 2 variation variation NN cord-013178-li1x1m25 478 3 and and CC cord-013178-li1x1m25 478 4 newly newly RB cord-013178-li1x1m25 478 5 emerging emerge VBG cord-013178-li1x1m25 478 6 genotypes genotype NNS cord-013178-li1x1m25 478 7 in in IN cord-013178-li1x1m25 478 8 China China NNP cord-013178-li1x1m25 478 9 Genetic genetic JJ cord-013178-li1x1m25 478 10 analysis analysis NN cord-013178-li1x1m25 478 11 of of IN cord-013178-li1x1m25 478 12 porcine porcine JJ cord-013178-li1x1m25 478 13 circovirus circovirus NNP cord-013178-li1x1m25 478 14 type type NN cord-013178-li1x1m25 478 15 2 2 CD cord-013178-li1x1m25 479 1 ( ( -LRB- cord-013178-li1x1m25 479 2 PCV2 PCV2 NNP cord-013178-li1x1m25 479 3 ) ) -RRB- cord-013178-li1x1m25 480 1 in in IN cord-013178-li1x1m25 480 2 Queensland Queensland NNP cord-013178-li1x1m25 480 3 Detection Detection NNP cord-013178-li1x1m25 480 4 and and CC cord-013178-li1x1m25 480 5 genotyping genotyping NN cord-013178-li1x1m25 480 6 of of IN cord-013178-li1x1m25 480 7 Porcine porcine JJ cord-013178-li1x1m25 480 8 circovirus circovirus NNP cord-013178-li1x1m25 480 9 2 2 CD cord-013178-li1x1m25 480 10 ( ( -LRB- cord-013178-li1x1m25 480 11 PCV-2 PCV-2 NNP cord-013178-li1x1m25 480 12 ) ) -RRB- cord-013178-li1x1m25 480 13 and and CC cord-013178-li1x1m25 480 14 detection detection NN cord-013178-li1x1m25 480 15 of of IN cord-013178-li1x1m25 480 16 Porcine porcine JJ cord-013178-li1x1m25 480 17 circovirus circovirus NNP cord-013178-li1x1m25 480 18 3 3 CD cord-013178-li1x1m25 480 19 ( ( -LRB- cord-013178-li1x1m25 480 20 PCV-3 PCV-3 NNP cord-013178-li1x1m25 480 21 ) ) -RRB- cord-013178-li1x1m25 480 22 in in IN cord-013178-li1x1m25 480 23 sera sera NNP cord-013178-li1x1m25 480 24 from from IN cord-013178-li1x1m25 480 25 fattening fatten VBG cord-013178-li1x1m25 480 26 pigs pig NNS cord-013178-li1x1m25 480 27 of of IN cord-013178-li1x1m25 480 28 different different JJ cord-013178-li1x1m25 480 29 European european JJ cord-013178-li1x1m25 480 30 countries country NNS cord-013178-li1x1m25 480 31 Genomic Genomic NNP cord-013178-li1x1m25 480 32 analysis analysis NN cord-013178-li1x1m25 480 33 of of IN cord-013178-li1x1m25 480 34 porcine porcine JJ cord-013178-li1x1m25 480 35 circovirus circovirus JJ cord-013178-li1x1m25 480 36 type type NN cord-013178-li1x1m25 480 37 2 2 CD cord-013178-li1x1m25 480 38 from from IN cord-013178-li1x1m25 480 39 southern southern JJ cord-013178-li1x1m25 480 40 China China NNP cord-013178-li1x1m25 480 41 Genetic genetic JJ cord-013178-li1x1m25 480 42 diversity diversity NN cord-013178-li1x1m25 480 43 and and CC cord-013178-li1x1m25 480 44 prevalence prevalence NN cord-013178-li1x1m25 480 45 of of IN cord-013178-li1x1m25 480 46 porcine porcine JJ cord-013178-li1x1m25 480 47 circovirus circovirus NN cord-013178-li1x1m25 480 48 type type NN cord-013178-li1x1m25 480 49 3 3 CD cord-013178-li1x1m25 480 50 ( ( -LRB- cord-013178-li1x1m25 480 51 PCV3 PCV3 NNP cord-013178-li1x1m25 480 52 ) ) -RRB- cord-013178-li1x1m25 480 53 and and CC cord-013178-li1x1m25 480 54 type type NN cord-013178-li1x1m25 480 55 2 2 CD cord-013178-li1x1m25 481 1 ( ( -LRB- cord-013178-li1x1m25 481 2 PCV2 PCV2 NNP cord-013178-li1x1m25 481 3 ) ) -RRB- cord-013178-li1x1m25 482 1 in in IN cord-013178-li1x1m25 482 2 the the DT cord-013178-li1x1m25 482 3 Midwest Midwest NNP cord-013178-li1x1m25 482 4 of of IN cord-013178-li1x1m25 482 5 the the DT cord-013178-li1x1m25 482 6 USA USA NNP cord-013178-li1x1m25 482 7 during during IN cord-013178-li1x1m25 482 8 Global global JJ cord-013178-li1x1m25 482 9 molecular molecular JJ cord-013178-li1x1m25 482 10 genetic genetic JJ cord-013178-li1x1m25 482 11 analysis analysis NN cord-013178-li1x1m25 482 12 of of IN cord-013178-li1x1m25 482 13 porcine porcine JJ cord-013178-li1x1m25 482 14 circovirus circovirus NNP cord-013178-li1x1m25 482 15 type type NN cord-013178-li1x1m25 482 16 2 2 CD cord-013178-li1x1m25 482 17 ( ( -LRB- cord-013178-li1x1m25 482 18 PCV2 PCV2 NNP cord-013178-li1x1m25 482 19 ) ) -RRB- cord-013178-li1x1m25 482 20 sequences sequence NNS cord-013178-li1x1m25 482 21 confirms confirm VBZ cord-013178-li1x1m25 482 22 the the DT cord-013178-li1x1m25 482 23 presence presence NN cord-013178-li1x1m25 482 24 of of IN cord-013178-li1x1m25 482 25 four four CD cord-013178-li1x1m25 482 26 main main JJ cord-013178-li1x1m25 482 27 PCV2 PCV2 NNP cord-013178-li1x1m25 482 28 genotypes genotype NNS cord-013178-li1x1m25 482 29 and and CC cord-013178-li1x1m25 482 30 reveals reveal VBZ cord-013178-li1x1m25 482 31 a a DT cord-013178-li1x1m25 482 32 rapid rapid JJ cord-013178-li1x1m25 482 33 increase increase NN cord-013178-li1x1m25 482 34 of of IN cord-013178-li1x1m25 482 35 PCV2d PCV2d NNP cord-013178-li1x1m25 482 36 Nucleotide Nucleotide NNP cord-013178-li1x1m25 482 37 Sequence sequence NN cord-013178-li1x1m25 482 38 of of IN cord-013178-li1x1m25 482 39 Porcine porcine JJ cord-013178-li1x1m25 482 40 Circovirus Circovirus NNP cord-013178-li1x1m25 482 41 Associated Associated NNP cord-013178-li1x1m25 482 42 with with IN cord-013178-li1x1m25 482 43 Postweaning Postweaning NNP cord-013178-li1x1m25 482 44 Multisystemic Multisystemic NNP cord-013178-li1x1m25 482 45 Wasting Wasting NNP cord-013178-li1x1m25 482 46 Syndrome Syndrome NNP cord-013178-li1x1m25 482 47 in in IN cord-013178-li1x1m25 482 48 Pigs pig NNS cord-013178-li1x1m25 483 1 The the DT cord-013178-li1x1m25 483 2 essential essential JJ cord-013178-li1x1m25 483 3 and and CC cord-013178-li1x1m25 483 4 nonessential nonessential JJ cord-013178-li1x1m25 483 5 transcription transcription NN cord-013178-li1x1m25 483 6 units unit NNS cord-013178-li1x1m25 483 7 for for IN cord-013178-li1x1m25 483 8 viral viral JJ cord-013178-li1x1m25 483 9 protein protein NN cord-013178-li1x1m25 483 10 synthesis synthesis NN cord-013178-li1x1m25 483 11 and and CC cord-013178-li1x1m25 483 12 DNA dna NN cord-013178-li1x1m25 483 13 replication replication NN cord-013178-li1x1m25 483 14 of of IN cord-013178-li1x1m25 483 15 porcine porcine JJ cord-013178-li1x1m25 483 16 circovirus circovirus JJ cord-013178-li1x1m25 483 17 type type NN cord-013178-li1x1m25 483 18 2 2 CD cord-013178-li1x1m25 483 19 Molecular molecular JJ cord-013178-li1x1m25 483 20 biology biology NN cord-013178-li1x1m25 483 21 of of IN cord-013178-li1x1m25 483 22 Porcine porcine JJ cord-013178-li1x1m25 483 23 circovirus circovirus NN cord-013178-li1x1m25 483 24 : : : cord-013178-li1x1m25 484 1 Analyses analysis NNS cord-013178-li1x1m25 484 2 of of IN cord-013178-li1x1m25 484 3 gene gene NN cord-013178-li1x1m25 484 4 expression expression NN cord-013178-li1x1m25 484 5 and and CC cord-013178-li1x1m25 484 6 viral viral JJ cord-013178-li1x1m25 484 7 replication replication NN cord-013178-li1x1m25 484 8 Replacement replacement NN cord-013178-li1x1m25 484 9 of of IN cord-013178-li1x1m25 484 10 the the DT cord-013178-li1x1m25 484 11 replication replication NN cord-013178-li1x1m25 484 12 factors factor NNS cord-013178-li1x1m25 484 13 of of IN cord-013178-li1x1m25 484 14 porcine porcine JJ cord-013178-li1x1m25 484 15 circovirus circovirus NN cord-013178-li1x1m25 484 16 ( ( -LRB- cord-013178-li1x1m25 484 17 PCV pcv NN cord-013178-li1x1m25 484 18 ) ) -RRB- cord-013178-li1x1m25 484 19 type type NN cord-013178-li1x1m25 484 20 2 2 CD cord-013178-li1x1m25 484 21 with with IN cord-013178-li1x1m25 484 22 those those DT cord-013178-li1x1m25 484 23 of of IN cord-013178-li1x1m25 484 24 PCV PCV NNP cord-013178-li1x1m25 484 25 type type NN cord-013178-li1x1m25 484 26 1 1 CD cord-013178-li1x1m25 484 27 greatly greatly RB cord-013178-li1x1m25 484 28 enhances enhance VBZ cord-013178-li1x1m25 484 29 viral viral JJ cord-013178-li1x1m25 484 30 replication replication NN cord-013178-li1x1m25 484 31 in in IN cord-013178-li1x1m25 484 32 vitro vitro FW cord-013178-li1x1m25 484 33 Open open JJ cord-013178-li1x1m25 484 34 reading reading NN cord-013178-li1x1m25 484 35 frame frame NN cord-013178-li1x1m25 484 36 2 2 CD cord-013178-li1x1m25 484 37 of of IN cord-013178-li1x1m25 484 38 porcine porcine JJ cord-013178-li1x1m25 484 39 circovirus circovirus NN cord-013178-li1x1m25 484 40 type type NN cord-013178-li1x1m25 484 41 2 2 CD cord-013178-li1x1m25 484 42 encodes encode VBZ cord-013178-li1x1m25 484 43 a a DT cord-013178-li1x1m25 484 44 major major JJ cord-013178-li1x1m25 484 45 capsid capsid NN cord-013178-li1x1m25 484 46 protein protein NN cord-013178-li1x1m25 484 47 A a DT cord-013178-li1x1m25 484 48 porcine porcine JJ cord-013178-li1x1m25 484 49 circovirus circovirus NN cord-013178-li1x1m25 484 50 type type NN cord-013178-li1x1m25 484 51 2 2 CD cord-013178-li1x1m25 484 52 ( ( -LRB- cord-013178-li1x1m25 484 53 PCV2 pcv2 JJ cord-013178-li1x1m25 484 54 ) ) -RRB- cord-013178-li1x1m25 484 55 mutant mutant JJ cord-013178-li1x1m25 484 56 with with IN cord-013178-li1x1m25 484 57 234 234 CD cord-013178-li1x1m25 484 58 amino amino JJ cord-013178-li1x1m25 484 59 acids acid NNS cord-013178-li1x1m25 484 60 in in IN cord-013178-li1x1m25 484 61 capsid capsid NN cord-013178-li1x1m25 484 62 protein protein NN cord-013178-li1x1m25 484 63 showed show VBD cord-013178-li1x1m25 484 64 more more JJR cord-013178-li1x1m25 484 65 virulence virulence NN cord-013178-li1x1m25 484 66 in in IN cord-013178-li1x1m25 484 67 vivo vivo NN cord-013178-li1x1m25 484 68 , , , cord-013178-li1x1m25 484 69 compared compare VBN cord-013178-li1x1m25 484 70 with with IN cord-013178-li1x1m25 484 71 classical classical JJ cord-013178-li1x1m25 484 72 PCV2a PCV2a NNP cord-013178-li1x1m25 484 73 / / SYM cord-013178-li1x1m25 484 74 b b TO cord-013178-li1x1m25 484 75 strain strain VB cord-013178-li1x1m25 484 76 Characterization characterization NN cord-013178-li1x1m25 484 77 of of IN cord-013178-li1x1m25 484 78 a a DT cord-013178-li1x1m25 484 79 Previously previously RB cord-013178-li1x1m25 484 80 Unidentified unidentified JJ cord-013178-li1x1m25 484 81 Viral Viral NNP cord-013178-li1x1m25 484 82 Protein Protein NNP cord-013178-li1x1m25 484 83 in in IN cord-013178-li1x1m25 484 84 Porcine Porcine NNP cord-013178-li1x1m25 484 85 Circovirus Circovirus NNP cord-013178-li1x1m25 484 86 Type Type NNP cord-013178-li1x1m25 484 87 2-Infected 2-infected CD cord-013178-li1x1m25 484 88 Cells Cells NNPS cord-013178-li1x1m25 484 89 and and CC cord-013178-li1x1m25 484 90 Its -PRON- PRP$ cord-013178-li1x1m25 484 91 Role role NN cord-013178-li1x1m25 484 92 in in IN cord-013178-li1x1m25 484 93 Virus Virus NNP cord-013178-li1x1m25 484 94 - - HYPH cord-013178-li1x1m25 484 95 Induced induce VBN cord-013178-li1x1m25 484 96 Apoptosis Apoptosis NNP cord-013178-li1x1m25 485 1 The the DT cord-013178-li1x1m25 485 2 ORF3 ORF3 NNP cord-013178-li1x1m25 485 3 protein protein NN cord-013178-li1x1m25 485 4 of of IN cord-013178-li1x1m25 485 5 porcine porcine JJ cord-013178-li1x1m25 485 6 circovirus circovirus JJ cord-013178-li1x1m25 485 7 type type NN cord-013178-li1x1m25 485 8 2 2 CD cord-013178-li1x1m25 485 9 is be VBZ cord-013178-li1x1m25 485 10 involved involve VBN cord-013178-li1x1m25 485 11 in in IN cord-013178-li1x1m25 485 12 viral viral JJ cord-013178-li1x1m25 485 13 pathogenesis pathogenesis NN cord-013178-li1x1m25 485 14 in in IN cord-013178-li1x1m25 485 15 vivo vivo NNP cord-013178-li1x1m25 485 16 Attenuation attenuation NN cord-013178-li1x1m25 485 17 of of IN cord-013178-li1x1m25 485 18 porcine porcine JJ cord-013178-li1x1m25 485 19 circovirus circovirus NNP cord-013178-li1x1m25 485 20 2 2 CD cord-013178-li1x1m25 485 21 in in IN cord-013178-li1x1m25 485 22 SPF SPF NNP cord-013178-li1x1m25 485 23 piglets piglet NNS cord-013178-li1x1m25 485 24 by by IN cord-013178-li1x1m25 485 25 abrogation abrogation NN cord-013178-li1x1m25 485 26 of of IN cord-013178-li1x1m25 485 27 ORF3 ORF3 NNP cord-013178-li1x1m25 485 28 function function NN cord-013178-li1x1m25 486 1 The the DT cord-013178-li1x1m25 486 2 ORF3 ORF3 NNP cord-013178-li1x1m25 486 3 protein protein NN cord-013178-li1x1m25 486 4 of of IN cord-013178-li1x1m25 486 5 porcine porcine JJ cord-013178-li1x1m25 486 6 circovirus circovirus NNP cord-013178-li1x1m25 486 7 type type NN cord-013178-li1x1m25 486 8 2 2 CD cord-013178-li1x1m25 486 9 interacts interact VBZ cord-013178-li1x1m25 486 10 with with IN cord-013178-li1x1m25 486 11 porcine porcine JJ cord-013178-li1x1m25 486 12 ubiquitin ubiquitin NN cord-013178-li1x1m25 486 13 E3 e3 NN cord-013178-li1x1m25 486 14 ligase ligase NN cord-013178-li1x1m25 486 15 Pirh2 pirh2 NN cord-013178-li1x1m25 486 16 and and CC cord-013178-li1x1m25 486 17 facilitates facilitate VBZ cord-013178-li1x1m25 486 18 p53 p53 NN cord-013178-li1x1m25 486 19 expression expression NN cord-013178-li1x1m25 486 20 in in IN cord-013178-li1x1m25 486 21 viral viral JJ cord-013178-li1x1m25 486 22 infection infection NN cord-013178-li1x1m25 487 1 Pirh2 Pirh2 NNP cord-013178-li1x1m25 487 2 , , , cord-013178-li1x1m25 487 3 a a DT cord-013178-li1x1m25 487 4 p53-induced p53-induce VBN cord-013178-li1x1m25 487 5 ubiquitin ubiquitin NN cord-013178-li1x1m25 487 6 - - HYPH cord-013178-li1x1m25 487 7 protein protein NN cord-013178-li1x1m25 487 8 ligase ligase NN cord-013178-li1x1m25 487 9 , , , cord-013178-li1x1m25 487 10 promotes promote VBZ cord-013178-li1x1m25 487 11 p53 p53 NN cord-013178-li1x1m25 487 12 degradation degradation NN cord-013178-li1x1m25 487 13 PCDH10 PCDH10 NNP cord-013178-li1x1m25 487 14 , , , cord-013178-li1x1m25 487 15 a a DT cord-013178-li1x1m25 487 16 novel novel NN cord-013178-li1x1m25 487 17 p53 p53 NN cord-013178-li1x1m25 487 18 transcriptional transcriptional JJ cord-013178-li1x1m25 487 19 target target NN cord-013178-li1x1m25 487 20 in in IN cord-013178-li1x1m25 487 21 regulating regulate VBG cord-013178-li1x1m25 487 22 cell cell NN cord-013178-li1x1m25 487 23 migration migration NN cord-013178-li1x1m25 488 1 Maf Maf NNP cord-013178-li1x1m25 488 2 transcriptionally transcriptionally RB cord-013178-li1x1m25 488 3 activates activate VBZ cord-013178-li1x1m25 488 4 the the DT cord-013178-li1x1m25 488 5 mouse mouse NN cord-013178-li1x1m25 488 6 p53 p53 NN cord-013178-li1x1m25 488 7 promoter promoter NN cord-013178-li1x1m25 488 8 and and CC cord-013178-li1x1m25 488 9 causes cause VBZ cord-013178-li1x1m25 488 10 a a DT cord-013178-li1x1m25 488 11 p53-dependent p53-dependent CD cord-013178-li1x1m25 488 12 cell cell NN cord-013178-li1x1m25 488 13 death death NN cord-013178-li1x1m25 488 14 p53 p53 NN cord-013178-li1x1m25 488 15 and and CC cord-013178-li1x1m25 488 16 Apoptosis apoptosis NN cord-013178-li1x1m25 488 17 : : : cord-013178-li1x1m25 489 1 It -PRON- PRP cord-013178-li1x1m25 489 2 's be VBZ cord-013178-li1x1m25 489 3 Not not RB cord-013178-li1x1m25 489 4 Just just RB cord-013178-li1x1m25 489 5 in in IN cord-013178-li1x1m25 489 6 the the DT cord-013178-li1x1m25 489 7 Nucleus nucleus NN cord-013178-li1x1m25 490 1 Anymore anymore RB cord-013178-li1x1m25 490 2 Porcine porcine JJ cord-013178-li1x1m25 490 3 circovirus circovirus JJ cord-013178-li1x1m25 490 4 type type NN cord-013178-li1x1m25 490 5 2 2 CD cord-013178-li1x1m25 490 6 ORF3 ORF3 NNP cord-013178-li1x1m25 490 7 protein protein NN cord-013178-li1x1m25 490 8 competes compete VBZ cord-013178-li1x1m25 490 9 with with IN cord-013178-li1x1m25 490 10 p53 p53 NN cord-013178-li1x1m25 490 11 in in IN cord-013178-li1x1m25 490 12 binding bind VBG cord-013178-li1x1m25 490 13 to to IN cord-013178-li1x1m25 490 14 Pirh2 pirh2 NN cord-013178-li1x1m25 490 15 and and CC cord-013178-li1x1m25 490 16 mediates mediate VBZ cord-013178-li1x1m25 490 17 the the DT cord-013178-li1x1m25 490 18 deregulation deregulation NN cord-013178-li1x1m25 490 19 of of IN cord-013178-li1x1m25 490 20 p53 p53 NN cord-013178-li1x1m25 490 21 homeostasis homeostasis NN cord-013178-li1x1m25 491 1 The the DT cord-013178-li1x1m25 491 2 ORF3 ORF3 NNP cord-013178-li1x1m25 491 3 protein protein NN cord-013178-li1x1m25 491 4 of of IN cord-013178-li1x1m25 491 5 porcine porcine JJ cord-013178-li1x1m25 491 6 circovirus circovirus NNP cord-013178-li1x1m25 491 7 type type NN cord-013178-li1x1m25 491 8 2 2 CD cord-013178-li1x1m25 491 9 promotes promote VBZ cord-013178-li1x1m25 491 10 secretion secretion NN cord-013178-li1x1m25 491 11 of of IN cord-013178-li1x1m25 492 1 IL-6 IL-6 NNP cord-013178-li1x1m25 492 2 and and CC cord-013178-li1x1m25 492 3 IL-8 IL-8 NNP cord-013178-li1x1m25 492 4 in in IN cord-013178-li1x1m25 492 5 porcine porcine JJ cord-013178-li1x1m25 492 6 epithelial epithelial JJ cord-013178-li1x1m25 492 7 cells cell NNS cord-013178-li1x1m25 492 8 by by IN cord-013178-li1x1m25 492 9 facilitating facilitate VBG cord-013178-li1x1m25 492 10 proteasomal proteasomal JJ cord-013178-li1x1m25 492 11 degradation degradation NN cord-013178-li1x1m25 492 12 of of IN cord-013178-li1x1m25 492 13 regulator regulator NN cord-013178-li1x1m25 492 14 of of IN cord-013178-li1x1m25 492 15 G g NN cord-013178-li1x1m25 492 16 protein protein NN cord-013178-li1x1m25 492 17 signalling signal VBG cord-013178-li1x1m25 492 18 16 16 CD cord-013178-li1x1m25 492 19 through through IN cord-013178-li1x1m25 492 20 physical physical JJ cord-013178-li1x1m25 492 21 interaction interaction NN cord-013178-li1x1m25 492 22 ORF3 ORF3 NNP cord-013178-li1x1m25 492 23 of of IN cord-013178-li1x1m25 492 24 porcine porcine JJ cord-013178-li1x1m25 492 25 circovirus circovirus NNP cord-013178-li1x1m25 492 26 2 2 CD cord-013178-li1x1m25 492 27 enhances enhance VBZ cord-013178-li1x1m25 492 28 the the DT cord-013178-li1x1m25 492 29 in in FW cord-013178-li1x1m25 492 30 vitro vitro FW cord-013178-li1x1m25 492 31 and and CC cord-013178-li1x1m25 492 32 in in IN cord-013178-li1x1m25 492 33 vivo vivo NN cord-013178-li1x1m25 492 34 spread spread NN cord-013178-li1x1m25 492 35 of of IN cord-013178-li1x1m25 492 36 the the DT cord-013178-li1x1m25 492 37 of of IN cord-013178-li1x1m25 492 38 the the DT cord-013178-li1x1m25 492 39 virus virus NN cord-013178-li1x1m25 492 40 T T NNP cord-013178-li1x1m25 492 41 lymphocyte lymphocyte NN cord-013178-li1x1m25 492 42 epitope epitope NN cord-013178-li1x1m25 492 43 mapping mapping NN cord-013178-li1x1m25 492 44 of of IN cord-013178-li1x1m25 492 45 porcine porcine JJ cord-013178-li1x1m25 492 46 circovirus circovirus JJ cord-013178-li1x1m25 492 47 type type NN cord-013178-li1x1m25 492 48 2 2 CD cord-013178-li1x1m25 492 49 . . . cord-013178-li1x1m25 493 1 Viral Viral NNP cord-013178-li1x1m25 493 2 Immunol Immunol NNP cord-013178-li1x1m25 493 3 Characterization Characterization NNP cord-013178-li1x1m25 493 4 of of IN cord-013178-li1x1m25 493 5 specific specific JJ cord-013178-li1x1m25 493 6 antigenic antigenic JJ cord-013178-li1x1m25 493 7 epitopes epitope NNS cord-013178-li1x1m25 493 8 and and CC cord-013178-li1x1m25 493 9 the the DT cord-013178-li1x1m25 493 10 nuclear nuclear JJ cord-013178-li1x1m25 493 11 export export NN cord-013178-li1x1m25 493 12 signal signal NN cord-013178-li1x1m25 493 13 of of IN cord-013178-li1x1m25 493 14 the the DT cord-013178-li1x1m25 493 15 Porcine porcine JJ cord-013178-li1x1m25 493 16 circovirus circovirus NNP cord-013178-li1x1m25 493 17 2 2 CD cord-013178-li1x1m25 493 18 ORF3 ORF3 NNP cord-013178-li1x1m25 493 19 protein protein NN cord-013178-li1x1m25 493 20 Analysis Analysis NNP cord-013178-li1x1m25 493 21 of of IN cord-013178-li1x1m25 493 22 Putative Putative NNP cord-013178-li1x1m25 493 23 ORF3 ORF3 NNP cord-013178-li1x1m25 493 24 Gene Gene NNP cord-013178-li1x1m25 493 25 Within within IN cord-013178-li1x1m25 493 26 Porcine Porcine NNP cord-013178-li1x1m25 493 27 Circovirus Circovirus NNP cord-013178-li1x1m25 493 28 Type type NN cord-013178-li1x1m25 493 29 2 2 CD cord-013178-li1x1m25 493 30 Peptides peptide NNS cord-013178-li1x1m25 493 31 mimicking mimic VBG cord-013178-li1x1m25 493 32 viral viral JJ cord-013178-li1x1m25 493 33 proteins protein NNS cord-013178-li1x1m25 493 34 of of IN cord-013178-li1x1m25 493 35 porcine porcine JJ cord-013178-li1x1m25 493 36 circovirus circovirus JJ cord-013178-li1x1m25 493 37 type type NN cord-013178-li1x1m25 493 38 2 2 CD cord-013178-li1x1m25 493 39 were be VBD cord-013178-li1x1m25 493 40 profiled profile VBN cord-013178-li1x1m25 493 41 by by IN cord-013178-li1x1m25 493 42 the the DT cord-013178-li1x1m25 493 43 spectrum spectrum NNP cord-013178-li1x1m25 493 44 of of IN cord-013178-li1x1m25 493 45 mouse mouse NN cord-013178-li1x1m25 493 46 anti anti JJ cord-013178-li1x1m25 493 47 - - JJ cord-013178-li1x1m25 493 48 PCV2 pcv2 JJ cord-013178-li1x1m25 493 49 antibodies antibody NNS cord-013178-li1x1m25 494 1 Versatile versatile JJ cord-013178-li1x1m25 494 2 carboxyl carboxyl NN cord-013178-li1x1m25 494 3 - - HYPH cord-013178-li1x1m25 494 4 terminus terminus NN cord-013178-li1x1m25 494 5 of of IN cord-013178-li1x1m25 494 6 capsid capsid JJ cord-013178-li1x1m25 494 7 protein protein NN cord-013178-li1x1m25 494 8 of of IN cord-013178-li1x1m25 494 9 porcine porcine JJ cord-013178-li1x1m25 494 10 circovirus circovirus NNP cord-013178-li1x1m25 494 11 type type NN cord-013178-li1x1m25 494 12 2 2 CD cord-013178-li1x1m25 494 13 were be VBD cord-013178-li1x1m25 494 14 recognized recognize VBN cord-013178-li1x1m25 494 15 by by IN cord-013178-li1x1m25 494 16 monoclonal monoclonal JJ cord-013178-li1x1m25 494 17 antibodies antibody NNS cord-013178-li1x1m25 494 18 with with IN cord-013178-li1x1m25 494 19 pluripotency pluripotency NN cord-013178-li1x1m25 494 20 of of IN cord-013178-li1x1m25 494 21 binding bind VBG cord-013178-li1x1m25 494 22 The the DT cord-013178-li1x1m25 494 23 Porcine Porcine NNP cord-013178-li1x1m25 494 24 Circovirus Circovirus NNP cord-013178-li1x1m25 494 25 Type type NN cord-013178-li1x1m25 494 26 2 2 CD cord-013178-li1x1m25 494 27 Nonstructural Nonstructural NNP cord-013178-li1x1m25 494 28 Protein Protein NNP cord-013178-li1x1m25 494 29 ORF3 ORF3 NNP cord-013178-li1x1m25 495 1 Induces induce VBZ cord-013178-li1x1m25 495 2 Apoptosis Apoptosis NNP cord-013178-li1x1m25 495 3 in in IN cord-013178-li1x1m25 495 4 Porcine Porcine NNP cord-013178-li1x1m25 495 5 Peripheral Peripheral NNP cord-013178-li1x1m25 495 6 Blood Blood NNP cord-013178-li1x1m25 495 7 Mononuclear Mononuclear NNP cord-013178-li1x1m25 495 8 Cells Cells NNPS cord-013178-li1x1m25 495 9 Light Light NNP cord-013178-li1x1m25 495 10 chain chain NN cord-013178-li1x1m25 495 11 multiple multiple JJ cord-013178-li1x1m25 495 12 myeloma myeloma NN cord-013178-li1x1m25 495 13 : : : cord-013178-li1x1m25 496 1 An an DT cord-013178-li1x1m25 496 2 evaluation evaluation NN cord-013178-li1x1m25 496 3 of of IN cord-013178-li1x1m25 496 4 its -PRON- PRP$ cord-013178-li1x1m25 496 5 biochemical biochemical JJ cord-013178-li1x1m25 496 6 investigations investigation NNS cord-013178-li1x1m25 496 7 International International NNP cord-013178-li1x1m25 496 8 Myeloma Myeloma NNP cord-013178-li1x1m25 496 9 Working Working NNP cord-013178-li1x1m25 496 10 Group Group NNP cord-013178-li1x1m25 496 11 guidelines guideline NNS cord-013178-li1x1m25 496 12 for for IN cord-013178-li1x1m25 496 13 serum serum NN cord-013178-li1x1m25 496 14 - - HYPH cord-013178-li1x1m25 496 15 free free JJ cord-013178-li1x1m25 496 16 light light JJ cord-013178-li1x1m25 496 17 chain chain NN cord-013178-li1x1m25 496 18 analysis analysis NN cord-013178-li1x1m25 496 19 in in IN cord-013178-li1x1m25 496 20 multiple multiple JJ cord-013178-li1x1m25 496 21 myeloma myeloma NN cord-013178-li1x1m25 496 22 and and CC cord-013178-li1x1m25 496 23 related related JJ cord-013178-li1x1m25 496 24 disorders disorder NNS cord-013178-li1x1m25 496 25 Treatment Treatment NNP cord-013178-li1x1m25 496 26 of of IN cord-013178-li1x1m25 496 27 AL AL NNP cord-013178-li1x1m25 496 28 amyloidosis amyloidosis NN cord-013178-li1x1m25 496 29 Suppression Suppression NNP cord-013178-li1x1m25 496 30 of of IN cord-013178-li1x1m25 496 31 lymphocyte lymphocyte NN cord-013178-li1x1m25 496 32 apoptosis apoptosis NN cord-013178-li1x1m25 496 33 in in IN cord-013178-li1x1m25 496 34 spleen spleen NNS cord-013178-li1x1m25 496 35 by by IN cord-013178-li1x1m25 496 36 CXCL13 CXCL13 NNS cord-013178-li1x1m25 496 37 after after IN cord-013178-li1x1m25 496 38 porcine porcine JJ cord-013178-li1x1m25 496 39 circovirus circovirus NN cord-013178-li1x1m25 496 40 type type NN cord-013178-li1x1m25 496 41 2 2 CD cord-013178-li1x1m25 496 42 infection infection NN cord-013178-li1x1m25 496 43 and and CC cord-013178-li1x1m25 496 44 regulatory regulatory JJ cord-013178-li1x1m25 496 45 mechanism mechanism NN cord-013178-li1x1m25 496 46 of of IN cord-013178-li1x1m25 496 47 CXCL13 CXCL13 NNP cord-013178-li1x1m25 496 48 expression expression NN cord-013178-li1x1m25 496 49 in in IN cord-013178-li1x1m25 496 50 pigs pig NNS cord-013178-li1x1m25 496 51 Apoptosis Apoptosis NNP cord-013178-li1x1m25 496 52 in in IN cord-013178-li1x1m25 496 53 lymphoid lymphoid JJ cord-013178-li1x1m25 496 54 organs organ NNS cord-013178-li1x1m25 496 55 of of IN cord-013178-li1x1m25 496 56 pigs pig NNS cord-013178-li1x1m25 496 57 naturally naturally RB cord-013178-li1x1m25 496 58 infected infect VBN cord-013178-li1x1m25 496 59 by by IN cord-013178-li1x1m25 496 60 porcine porcine JJ cord-013178-li1x1m25 496 61 circovirus circovirus NN cord-013178-li1x1m25 496 62 type type NN cord-013178-li1x1m25 496 63 2 2 CD cord-013178-li1x1m25 496 64 Immunosuppression Immunosuppression NNP cord-013178-li1x1m25 496 65 in in IN cord-013178-li1x1m25 496 66 postweaning postweane VBG cord-013178-li1x1m25 496 67 multisystemic multisystemic NNP cord-013178-li1x1m25 496 68 wasting waste VBG cord-013178-li1x1m25 496 69 syndrome syndrome NN cord-013178-li1x1m25 496 70 affected affect VBD cord-013178-li1x1m25 496 71 pigs pig NNS cord-013178-li1x1m25 496 72 Pathology Pathology NNP cord-013178-li1x1m25 496 73 of of IN cord-013178-li1x1m25 496 74 African african JJ cord-013178-li1x1m25 496 75 swine swine NN cord-013178-li1x1m25 496 76 fever fever NN cord-013178-li1x1m25 496 77 : : : cord-013178-li1x1m25 496 78 The the DT cord-013178-li1x1m25 496 79 role role NN cord-013178-li1x1m25 496 80 of of IN cord-013178-li1x1m25 496 81 monocyte monocyte NNP cord-013178-li1x1m25 496 82 - - HYPH cord-013178-li1x1m25 496 83 macrophage macrophage NN cord-013178-li1x1m25 496 84 Atrophy atrophy NN cord-013178-li1x1m25 496 85 of of IN cord-013178-li1x1m25 496 86 primary primary JJ cord-013178-li1x1m25 496 87 lymphoid lymphoid JJ cord-013178-li1x1m25 496 88 organs organ NNS cord-013178-li1x1m25 496 89 induced induce VBN cord-013178-li1x1m25 496 90 by by IN cord-013178-li1x1m25 496 91 Marek Marek NNP cord-013178-li1x1m25 496 92 's 's POS cord-013178-li1x1m25 496 93 disease disease NN cord-013178-li1x1m25 496 94 virus virus NN cord-013178-li1x1m25 496 95 during during IN cord-013178-li1x1m25 496 96 early early JJ cord-013178-li1x1m25 496 97 infection infection NN cord-013178-li1x1m25 496 98 is be VBZ cord-013178-li1x1m25 496 99 associated associate VBN cord-013178-li1x1m25 496 100 with with IN cord-013178-li1x1m25 496 101 increased increase VBN cord-013178-li1x1m25 496 102 apoptosis apoptosis NN cord-013178-li1x1m25 496 103 , , , cord-013178-li1x1m25 496 104 inhibition inhibition NN cord-013178-li1x1m25 496 105 of of IN cord-013178-li1x1m25 496 106 cell cell NN cord-013178-li1x1m25 496 107 proliferation proliferation NN cord-013178-li1x1m25 496 108 and and CC cord-013178-li1x1m25 496 109 a a DT cord-013178-li1x1m25 496 110 severe severe JJ cord-013178-li1x1m25 496 111 B b NN cord-013178-li1x1m25 496 112 - - HYPH cord-013178-li1x1m25 496 113 lymphopenia lymphopenia NNP cord-013178-li1x1m25 496 114 Transcriptomic Transcriptomic NNP cord-013178-li1x1m25 496 115 characteristics characteristic NNS cord-013178-li1x1m25 496 116 of of IN cord-013178-li1x1m25 496 117 bronchoalveolar bronchoalveolar NNP cord-013178-li1x1m25 496 118 lavage lavage NN cord-013178-li1x1m25 496 119 fluid fluid NN cord-013178-li1x1m25 496 120 and and CC cord-013178-li1x1m25 496 121 peripheral peripheral JJ cord-013178-li1x1m25 496 122 blood blood NN cord-013178-li1x1m25 496 123 mononuclear mononuclear NN cord-013178-li1x1m25 496 124 cells cell NNS cord-013178-li1x1m25 496 125 in in IN cord-013178-li1x1m25 496 126 COVID-19 covid-19 CD cord-013178-li1x1m25 496 127 patients patient NNS cord-013178-li1x1m25 496 128 Apoptosis Apoptosis NNP cord-013178-li1x1m25 496 129 and and CC cord-013178-li1x1m25 496 130 proliferative proliferative JJ cord-013178-li1x1m25 496 131 activity activity NN cord-013178-li1x1m25 496 132 in in IN cord-013178-li1x1m25 496 133 lymph lymph NN cord-013178-li1x1m25 496 134 node node NN cord-013178-li1x1m25 496 135 reaction reaction NN cord-013178-li1x1m25 496 136 in in IN cord-013178-li1x1m25 496 137 postweaning postweane VBG cord-013178-li1x1m25 496 138 multisystemic multisystemic NNP cord-013178-li1x1m25 496 139 wasting waste VBG cord-013178-li1x1m25 496 140 syndrome syndrome NN cord-013178-li1x1m25 496 141 ( ( -LRB- cord-013178-li1x1m25 496 142 PMWS PMWS NNP cord-013178-li1x1m25 496 143 ) ) -RRB- cord-013178-li1x1m25 496 144 . . . cord-013178-li1x1m25 497 1 Veterinary Veterinary NNP cord-013178-li1x1m25 497 2 Immunology Immunology NNP cord-013178-li1x1m25 497 3 and and CC cord-013178-li1x1m25 497 4 Immunopathology Immunopathology NNP cord-013178-li1x1m25 497 5 Porcine porcine JJ cord-013178-li1x1m25 497 6 circovirus circovirus JJ cord-013178-li1x1m25 497 7 type type NN cord-013178-li1x1m25 497 8 2 2 CD cord-013178-li1x1m25 498 1 ( ( -LRB- cord-013178-li1x1m25 498 2 PCV2 PCV2 NNP cord-013178-li1x1m25 498 3 ) ) -RRB- cord-013178-li1x1m25 498 4 causes cause VBZ cord-013178-li1x1m25 498 5 apoptosis apoptosis NN cord-013178-li1x1m25 498 6 in in IN cord-013178-li1x1m25 498 7 experimentally experimentally RB cord-013178-li1x1m25 498 8 inoculated inoculate VBN cord-013178-li1x1m25 498 9 BALB BALB NNP cord-013178-li1x1m25 498 10 / / SYM cord-013178-li1x1m25 498 11 c c NN cord-013178-li1x1m25 498 12 mice mouse NNS cord-013178-li1x1m25 499 1 Granzyme Granzyme NNP cord-013178-li1x1m25 499 2 B B NNP cord-013178-li1x1m25 499 3 - - HYPH cord-013178-li1x1m25 499 4 Activated activate VBN cord-013178-li1x1m25 499 5 p53 p53 NN cord-013178-li1x1m25 499 6 Interacts interact VBZ cord-013178-li1x1m25 499 7 with with IN cord-013178-li1x1m25 499 8 Bcl-2 Bcl-2 NNP cord-013178-li1x1m25 499 9 To to TO cord-013178-li1x1m25 499 10 Promote promote VB cord-013178-li1x1m25 499 11 Cytotoxic Cytotoxic NNP cord-013178-li1x1m25 499 12 Lymphocyte Lymphocyte NNP cord-013178-li1x1m25 499 13 - - HYPH cord-013178-li1x1m25 499 14 Mediated mediate VBN cord-013178-li1x1m25 499 15 Apoptosis apoptosis NN cord-013178-li1x1m25 499 16 Calcium calcium NN cord-013178-li1x1m25 499 17 dependence dependence NN cord-013178-li1x1m25 499 18 of of IN cord-013178-li1x1m25 499 19 glucocorticoid glucocorticoid NN cord-013178-li1x1m25 499 20 - - HYPH cord-013178-li1x1m25 499 21 induced induce VBN cord-013178-li1x1m25 499 22 lymphocytolysis lymphocytolysis NNP cord-013178-li1x1m25 499 23 Stress stress NN cord-013178-li1x1m25 499 24 - - HYPH cord-013178-li1x1m25 499 25 induced induce VBN cord-013178-li1x1m25 499 26 enhancement enhancement NN cord-013178-li1x1m25 499 27 of of IN cord-013178-li1x1m25 499 28 activity activity NN cord-013178-li1x1m25 499 29 of of IN cord-013178-li1x1m25 499 30 lymphocyte lymphocyte NN cord-013178-li1x1m25 499 31 lysosomal lysosomal NN cord-013178-li1x1m25 499 32 enzymes enzyme NNS cord-013178-li1x1m25 499 33 in in IN cord-013178-li1x1m25 499 34 pigs pig NNS cord-013178-li1x1m25 499 35 of of IN cord-013178-li1x1m25 499 36 different different JJ cord-013178-li1x1m25 499 37 stress stress NN cord-013178-li1x1m25 499 38 - - HYPH cord-013178-li1x1m25 499 39 susceptibility susceptibility NN cord-013178-li1x1m25 499 40 Studies study NNS cord-013178-li1x1m25 499 41 on on IN cord-013178-li1x1m25 499 42 porcine porcine JJ cord-013178-li1x1m25 499 43 circovirus circovirus JJ cord-013178-li1x1m25 499 44 type type NN cord-013178-li1x1m25 499 45 2 2 CD cord-013178-li1x1m25 499 46 vaccination vaccination NN cord-013178-li1x1m25 499 47 of of IN cord-013178-li1x1m25 499 48 5-day 5-day JJ cord-013178-li1x1m25 499 49 - - HYPH cord-013178-li1x1m25 499 50 old old JJ cord-013178-li1x1m25 499 51 piglets piglet NNS cord-013178-li1x1m25 500 1 Inactivated inactivate VBN cord-013178-li1x1m25 501 1 PCV2 pcv2 IN cord-013178-li1x1m25 501 2 one one CD cord-013178-li1x1m25 501 3 shot shot NN cord-013178-li1x1m25 501 4 vaccine vaccine NN cord-013178-li1x1m25 501 5 applied apply VBN cord-013178-li1x1m25 501 6 in in IN cord-013178-li1x1m25 501 7 3-week 3-week JJ cord-013178-li1x1m25 501 8 - - JJ cord-013178-li1x1m25 501 9 old old JJ cord-013178-li1x1m25 501 10 piglets piglet NNS cord-013178-li1x1m25 501 11 : : : cord-013178-li1x1m25 502 1 Improvement improvement NN cord-013178-li1x1m25 502 2 of of IN cord-013178-li1x1m25 502 3 production production NN cord-013178-li1x1m25 502 4 parameters parameter NNS cord-013178-li1x1m25 502 5 and and CC cord-013178-li1x1m25 502 6 interaction interaction NN cord-013178-li1x1m25 502 7 with with IN cord-013178-li1x1m25 502 8 maternally maternally RB cord-013178-li1x1m25 502 9 derived derive VBN cord-013178-li1x1m25 502 10 immunity immunity NN cord-013178-li1x1m25 502 11 Ten ten CD cord-013178-li1x1m25 502 12 years year NNS cord-013178-li1x1m25 502 13 of of IN cord-013178-li1x1m25 502 14 PCV2 pcv2 JJ cord-013178-li1x1m25 502 15 vaccines vaccine NNS cord-013178-li1x1m25 502 16 and and CC cord-013178-li1x1m25 502 17 vaccination vaccination NN cord-013178-li1x1m25 503 1 : : : cord-013178-li1x1m25 503 2 Is be VBZ cord-013178-li1x1m25 503 3 eradication eradication NN cord-013178-li1x1m25 503 4 a a DT cord-013178-li1x1m25 503 5 possibility possibility NN cord-013178-li1x1m25 503 6 ? ? . cord-013178-li1x1m25 504 1 Fetal fetal JJ cord-013178-li1x1m25 504 2 infections infection NNS cord-013178-li1x1m25 504 3 and and CC cord-013178-li1x1m25 504 4 antibody antibody NN cord-013178-li1x1m25 504 5 profiles profile NNS cord-013178-li1x1m25 504 6 in in IN cord-013178-li1x1m25 504 7 pigs pig NNS cord-013178-li1x1m25 504 8 naturally naturally RB cord-013178-li1x1m25 504 9 infected infect VBN cord-013178-li1x1m25 504 10 with with IN cord-013178-li1x1m25 504 11 porcine porcine JJ cord-013178-li1x1m25 504 12 circovirus circovirus JJ cord-013178-li1x1m25 504 13 type type NN cord-013178-li1x1m25 504 14 2 2 CD cord-013178-li1x1m25 505 1 ( ( -LRB- cord-013178-li1x1m25 505 2 PCV2 pcv2 JJ cord-013178-li1x1m25 505 3 ) ) -RRB- cord-013178-li1x1m25 505 4 Environmental environmental JJ cord-013178-li1x1m25 505 5 distribution distribution NN cord-013178-li1x1m25 505 6 of of IN cord-013178-li1x1m25 505 7 Porcine Porcine NNP cord-013178-li1x1m25 505 8 Circovirus Circovirus NNP cord-013178-li1x1m25 505 9 Type type NN cord-013178-li1x1m25 505 10 2 2 CD cord-013178-li1x1m25 505 11 ( ( -LRB- cord-013178-li1x1m25 505 12 PCV2 PCV2 NNP cord-013178-li1x1m25 505 13 ) ) -RRB- cord-013178-li1x1m25 505 14 in in IN cord-013178-li1x1m25 505 15 swine swine NN cord-013178-li1x1m25 505 16 herds herd NNS cord-013178-li1x1m25 505 17 with with IN cord-013178-li1x1m25 505 18 natural natural JJ cord-013178-li1x1m25 505 19 infection infection NN cord-013178-li1x1m25 506 1 The the DT cord-013178-li1x1m25 506 2 2.3-angstrom 2.3-angstrom CD cord-013178-li1x1m25 506 3 structure structure NN cord-013178-li1x1m25 506 4 of of IN cord-013178-li1x1m25 506 5 porcine porcine JJ cord-013178-li1x1m25 506 6 circovirus circovirus NNP cord-013178-li1x1m25 506 7 2 2 CD cord-013178-li1x1m25 506 8 One one CD cord-013178-li1x1m25 506 9 dose dose NN cord-013178-li1x1m25 506 10 of of IN cord-013178-li1x1m25 506 11 a a DT cord-013178-li1x1m25 506 12 porcine porcine JJ cord-013178-li1x1m25 506 13 circovirus circovirus NN cord-013178-li1x1m25 506 14 2 2 CD cord-013178-li1x1m25 506 15 ( ( -LRB- cord-013178-li1x1m25 506 16 PCV2 pcv2 JJ cord-013178-li1x1m25 506 17 ) ) -RRB- cord-013178-li1x1m25 506 18 sub sub JJ cord-013178-li1x1m25 506 19 - - JJ cord-013178-li1x1m25 506 20 unit unit NN cord-013178-li1x1m25 506 21 vaccine vaccine NN cord-013178-li1x1m25 506 22 administered administer VBN cord-013178-li1x1m25 506 23 to to IN cord-013178-li1x1m25 506 24 3-week 3-week JJ cord-013178-li1x1m25 506 25 - - JJ cord-013178-li1x1m25 506 26 old old JJ cord-013178-li1x1m25 506 27 conventional conventional JJ cord-013178-li1x1m25 506 28 piglets piglet NNS cord-013178-li1x1m25 506 29 elicits elicit VBZ cord-013178-li1x1m25 506 30 cell cell NN cord-013178-li1x1m25 506 31 - - HYPH cord-013178-li1x1m25 506 32 mediated mediate VBN cord-013178-li1x1m25 506 33 immunity immunity NN cord-013178-li1x1m25 506 34 and and CC cord-013178-li1x1m25 506 35 significantly significantly RB cord-013178-li1x1m25 506 36 reduces reduce VBZ cord-013178-li1x1m25 506 37 PCV2 pcv2 JJ cord-013178-li1x1m25 506 38 viremia viremia NN cord-013178-li1x1m25 506 39 in in IN cord-013178-li1x1m25 506 40 an an DT cord-013178-li1x1m25 506 41 experimental experimental JJ cord-013178-li1x1m25 506 42 model model NN cord-013178-li1x1m25 506 43 Immunogenicity Immunogenicity NNP cord-013178-li1x1m25 506 44 of of IN cord-013178-li1x1m25 506 45 empty empty JJ cord-013178-li1x1m25 506 46 capsids capsid NNS cord-013178-li1x1m25 506 47 of of IN cord-013178-li1x1m25 506 48 porcine porcine JJ cord-013178-li1x1m25 506 49 circovius circovius NN cord-013178-li1x1m25 506 50 type type NN cord-013178-li1x1m25 506 51 2 2 CD cord-013178-li1x1m25 506 52 produced produce VBN cord-013178-li1x1m25 506 53 in in IN cord-013178-li1x1m25 506 54 insect insect NN cord-013178-li1x1m25 506 55 cells cell NNS cord-013178-li1x1m25 507 1 Rüütel Rüütel NNP cord-013178-li1x1m25 507 2 Boudinot Boudinot NNP cord-013178-li1x1m25 507 3 , , , cord-013178-li1x1m25 507 4 S. S. NNP cord-013178-li1x1m25 507 5 Porcine porcine JJ cord-013178-li1x1m25 507 6 circovirus circovirus JJ cord-013178-li1x1m25 507 7 type type NN cord-013178-li1x1m25 507 8 2 2 CD cord-013178-li1x1m25 507 9 ORF3 ORF3 NNP cord-013178-li1x1m25 507 10 protein protein NN cord-013178-li1x1m25 507 11 induces induce VBZ cord-013178-li1x1m25 507 12 apoptosis apoptosis NN cord-013178-li1x1m25 507 13 in in IN cord-013178-li1x1m25 507 14 melanoma melanoma NN cord-013178-li1x1m25 507 15 cells cell NNS cord-013178-li1x1m25 507 16 Antigenic antigenic JJ cord-013178-li1x1m25 507 17 subtyping subtyping NN cord-013178-li1x1m25 507 18 and and CC cord-013178-li1x1m25 507 19 epitopes epitope NNS cord-013178-li1x1m25 507 20 ' ' POS cord-013178-li1x1m25 507 21 competition competition NN cord-013178-li1x1m25 507 22 analysis analysis NN cord-013178-li1x1m25 507 23 of of IN cord-013178-li1x1m25 507 24 porcine porcine JJ cord-013178-li1x1m25 507 25 circovirus circovirus NNP cord-013178-li1x1m25 507 26 type type NN cord-013178-li1x1m25 507 27 2 2 CD cord-013178-li1x1m25 507 28 using use VBG cord-013178-li1x1m25 507 29 monoclonal monoclonal JJ cord-013178-li1x1m25 507 30 antibodies antibody NNS cord-013178-li1x1m25 507 31 Identification Identification NNP cord-013178-li1x1m25 507 32 of of IN cord-013178-li1x1m25 507 33 one one CD cord-013178-li1x1m25 507 34 critical critical JJ cord-013178-li1x1m25 507 35 amino amino NN cord-013178-li1x1m25 507 36 acid acid NN cord-013178-li1x1m25 507 37 that that WDT cord-013178-li1x1m25 507 38 determines determine VBZ cord-013178-li1x1m25 507 39 a a DT cord-013178-li1x1m25 507 40 conformational conformational JJ cord-013178-li1x1m25 507 41 neutralizing neutralize VBG cord-013178-li1x1m25 507 42 epitope epitope NN cord-013178-li1x1m25 507 43 in in IN cord-013178-li1x1m25 507 44 the the DT cord-013178-li1x1m25 507 45 capsid capsid JJ cord-013178-li1x1m25 507 46 protein protein NN cord-013178-li1x1m25 507 47 of of IN cord-013178-li1x1m25 507 48 porcine porcine JJ cord-013178-li1x1m25 507 49 circovirus circovirus JJ cord-013178-li1x1m25 507 50 type type NN cord-013178-li1x1m25 507 51 2 2 CD cord-013178-li1x1m25 507 52 Fine fine JJ cord-013178-li1x1m25 507 53 mapping mapping NN cord-013178-li1x1m25 507 54 of of IN cord-013178-li1x1m25 507 55 antigenic antigenic JJ cord-013178-li1x1m25 507 56 epitopes epitope NNS cord-013178-li1x1m25 507 57 on on IN cord-013178-li1x1m25 507 58 capsid capsid JJ cord-013178-li1x1m25 507 59 proteins protein NNS cord-013178-li1x1m25 507 60 of of IN cord-013178-li1x1m25 507 61 porcine porcine JJ cord-013178-li1x1m25 507 62 circovirus circovirus NN cord-013178-li1x1m25 507 63 , , , cord-013178-li1x1m25 507 64 and and CC cord-013178-li1x1m25 507 65 antigenic antigenic JJ cord-013178-li1x1m25 507 66 phenotype phenotype NN cord-013178-li1x1m25 507 67 of of IN cord-013178-li1x1m25 507 68 porcine porcine JJ cord-013178-li1x1m25 507 69 circovirus circovirus JJ cord-013178-li1x1m25 507 70 type type NN cord-013178-li1x1m25 507 71 2 2 CD cord-013178-li1x1m25 508 1 In in IN cord-013178-li1x1m25 508 2 vitro vitro FW cord-013178-li1x1m25 508 3 and and CC cord-013178-li1x1m25 508 4 in in IN cord-013178-li1x1m25 508 5 silico silico NN cord-013178-li1x1m25 508 6 studies study NNS cord-013178-li1x1m25 508 7 reveal reveal VBP cord-013178-li1x1m25 508 8 capsid capsid NN cord-013178-li1x1m25 508 9 - - HYPH cord-013178-li1x1m25 508 10 mutant mutant JJ cord-013178-li1x1m25 508 11 Porcine Porcine NNP cord-013178-li1x1m25 508 12 circovirus circovirus NNP cord-013178-li1x1m25 508 13 2b 2b CD cord-013178-li1x1m25 508 14 with with IN cord-013178-li1x1m25 508 15 novel novel NN cord-013178-li1x1m25 508 16 cytopathogenic cytopathogenic NNP cord-013178-li1x1m25 508 17 and and CC cord-013178-li1x1m25 508 18 structural structural JJ cord-013178-li1x1m25 508 19 characteristics characteristic NNS cord-013178-li1x1m25 508 20 Structure Structure NNP cord-013178-li1x1m25 508 21 analysis analysis NN cord-013178-li1x1m25 508 22 of of IN cord-013178-li1x1m25 508 23 capsid capsid NN cord-013178-li1x1m25 508 24 protein protein NN cord-013178-li1x1m25 508 25 of of IN cord-013178-li1x1m25 508 26 Porcine porcine JJ cord-013178-li1x1m25 508 27 circovirus circovirus JJ cord-013178-li1x1m25 508 28 type type NN cord-013178-li1x1m25 508 29 2 2 CD cord-013178-li1x1m25 508 30 from from IN cord-013178-li1x1m25 508 31 pigs pig NNS cord-013178-li1x1m25 508 32 with with IN cord-013178-li1x1m25 508 33 systemic systemic JJ cord-013178-li1x1m25 508 34 disease disease NN cord-013178-li1x1m25 508 35 Antibody Antibody NNP cord-013178-li1x1m25 508 36 recognition recognition NN cord-013178-li1x1m25 508 37 of of IN cord-013178-li1x1m25 508 38 porcine porcine JJ cord-013178-li1x1m25 508 39 circovirus circovirus JJ cord-013178-li1x1m25 508 40 type type NN cord-013178-li1x1m25 508 41 2 2 CD cord-013178-li1x1m25 508 42 capsid capsid NN cord-013178-li1x1m25 508 43 protein protein NN cord-013178-li1x1m25 508 44 epitopes epitope NNS cord-013178-li1x1m25 508 45 after after IN cord-013178-li1x1m25 508 46 vaccination vaccination NN cord-013178-li1x1m25 508 47 , , , cord-013178-li1x1m25 508 48 infection infection NN cord-013178-li1x1m25 508 49 , , , cord-013178-li1x1m25 508 50 and and CC cord-013178-li1x1m25 508 51 disease disease NN cord-013178-li1x1m25 508 52 Viruses virus NNS cord-013178-li1x1m25 508 53 2020 2020 CD cord-013178-li1x1m25 508 54 , , , cord-013178-li1x1m25 508 55 12 12 CD cord-013178-li1x1m25 508 56 _SP