id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-347053-m5m4zgfy	Pharo, Elizabeth A.	Host–Pathogen Responses to Pandemic Influenza H1N1pdm09 in a Human Respiratory Airway Model	2020-06-24		.txt	text/plain	9450	554	50	wdNHBE cells produced an innate immune response to IAV-infection with increased transcription of proand anti-inflammatory cytokines and chemokines and the antiviral viperin but reduced expression of the mucin-encoding MUC5B, which may impair mucociliary clearance. The cytopathic effect of H1N1pdm09 included damage to the airway epithelium, the induction of innate immune responses including the expression of pro-and anti-inflammatory cytokines and chemokines and antiviral genes and proteins, consistent with pulmonary host defense. Key genes upregulated in our H1N1pdm09 in vitro challenge model, mimic the innate immune and inflammatory response in human patients in vivo infected with the 2009 pandemic IAV. The more than 350-fold induction of the antiviral-encoding RSAD2 (viperin) gene in our pandemic IAV-infected wdNHBE cells confirms the antiviral response of the airway epithelium in vitro. The disruption of the airway epithelium by IAV H1N1pdm09 and poly(I:C), plus the induction of the innate immune response and antiviral, and pro-and anti-inflammatory genes demonstrated the viability of this model to investigate pandemic influenza.	./cache/cord-347053-m5m4zgfy.txt	./txt/cord-347053-m5m4zgfy.txt