id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-336536-ie5ok0lz	Yeh, Ming Te	Mapping Attenuation Determinants in Enterovirus-D68	2020-08-08		.txt	text/plain	8258	401	56	A single amino acid change from isoleucine to valine at position 88 in VP3 attenuated neurovirulence by reducing virus replication in the brain and spinal cord of infected mice. To identify the potential virulence determinant, sequences of NR-49129, NR-49130, NR-49131 and CA/14-4231 were aligned to reveal conserved genetic changes that we defined as nucleotides in 5 -UTR and amino acids in the coding region that are present only in the non-pathogenic strain but not in pathogenic ones (Supplementary Data S1 and S2). While mutation VP1-L1P allowed a 50% survival of infected mice, VP3-I88V did not cause disease, suggesting its role as major virulence determinant for EV-D68 in this mouse model ( Figure 5E ). While mutation VP1-L1P allowed a 50% survival of infected mice, VP3-I88V did not cause disease, suggesting its role as major virulence determinant for EV-D68 in this mouse model ( Figure 5E ).	./cache/cord-336536-ie5ok0lz.txt	./txt/cord-336536-ie5ok0lz.txt