id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-331414-i0oxm5mr	Kautz, Tiffany F.	A Low Fidelity Virus Shows Increased Recombination during the Removal of an Alphavirus Reporter Gene	2020-06-19		.txt	text/plain	5064	241	45	To examine this, GFP was inserted into TC-83, a live-attenuated vaccine for the alphavirus Venezuelan equine encephalitis virus, as well as a low-fidelity variant of TC-83, and passaged until fluorescence was no longer observed. To examine reporter gene stability, three independent replicates of TC-83 GFP and low-fidelity TC-83 GFP were passaged 10 times on Vero cells using an approximate MOI of 0.5 ( Figure 1A ). All three TC-83 GFP replicates contained deletion variants present at a low frequency that were able to selectively remove the GFP reporter gene by passage 10 (Figure 2A) . To better understand the mechanisms underlying the conserved removal of the low-fidelity TC-83 GFP gene, M-fold was used to determine the RNA secondary structure of the virus genome ( Figure 4C-E) . To characterize the role of RNA structure of the virus genome in RNA recombination site selection, we modeled the three most common deletions that occurred at high levels during low-fidelity TC-83 GFP passaging.	./cache/cord-331414-i0oxm5mr.txt	./txt/cord-331414-i0oxm5mr.txt