id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-328259-3g4klpyg	Guajardo-Leiva, Sergio	Metagenomic Insights into the Sewage RNA Virosphere of a Large City	2020-09-21		.txt	text/plain	7626	370	47	Despite the overrepresentation of dsRNA viruses, our results show that Santiago's sewage RNA virosphere was composed mostly of unknown sequences (88%), while known viral sequences were dominated by viruses that infect bacteria (60%), invertebrates (37%) and humans (2.4%). Viral sequences identified as Partitiviridae-like viruses included in the "unclassified RNA viruses ShiM-2016" category in the NCBI taxonomy (~25% abundance; Figure 2B ) and Totiviriade family were also highly abundant in treated and untreated sewage samples from the EU [5, 7] . Therefore, the abundance of these viruses in the Trebal metagenome can expand the known sequence space associated with this family (only 10 genomes are currently available in the NCBI database) and contribute to a better understanding of the bacteriophage biology related to RNA genomes. Taken together, our results show that metagenomic surveys of RNA viruses in sewage samples and the use of HMMs could uncover extraordinary viral diversity through the detection of remote homologs in these human-impacted environments.	./cache/cord-328259-3g4klpyg.txt	./txt/cord-328259-3g4klpyg.txt