id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-297339-et2305rz	Lauber, Chris	Genetics-Based Classification of Filoviruses Calls for Expanded Sampling of Genomic Sequences	2012-08-31		.txt	text/plain	4480	235	46	In DEmARC, virus clusters are delimited objectively by devising a universal family-wide threshold on intra-cluster genetic divergence of viruses that is specific for each level of the classification. Based on our experience with other virus families, we conclude that the current sampling of filovirus genomic sequences needs to be considerably expanded in order to resolve these uncertainties in the framework of genetics-based classification. The DEmARC specifics include (i) the use of pairwise evolutionary distances (PEDs) instead of uncorrected p-distances, and (ii) a quantitative method to devise taxon levels and associated PED thresholds for virus clustering in a systematic and family-wide manner. The first selected threshold (PED of 0.120) results in seven clusters ( Figure 1B ) that match the official or tentative ICTV species of the family Filoviridae. In the high-sampling case of picornaviruses, no PED values with zero frequency are observed which suggests that the current sampling of filovirus genome sequences may strongly underestimate the natural genetic diversity in the family.	./cache/cord-297339-et2305rz.txt	./txt/cord-297339-et2305rz.txt