id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-281837-knswbb2d	Chang, Chia-Wen	Daphne Genkwa Sieb. et Zucc. Water-Soluble Extracts Act on Enterovirus 71 by Inhibiting Viral Entry	2012-04-11		.txt	text/plain	4915	293	57	An assay to measure the inhibition of virus-induced cell death in RD cells was first employed using 1 mg/mL of herbal extracts followed by EC 50 determination if the candidates possessed antiviral activity from the primary screening. DGFW but not DMSO protected the cells from virus-induced CPE (panel E versus F, Figure 2 ), indicating that DGFW inhibited viral replication. To test the mechanism of inhibition of viral entry into host cells by DGFW, we performed attachment and penetration assays. Unbound DGFW was removed, the cells were then infected with EV71 for 1 h, and the progeny viruses in the cells and culture medium were harvested for plaque assay at 10 h p.i. Similarly, EV71 was pretreated with DGFW or DMSO for 30 min at 37 °C, and the infectivity of the treated virus was then determined by plaque assay (Figure 6 ). A neutralization assay (EC 50 ) was used to test the antiviral efficacy of extracts or compounds by measuring the inhibition of CPE induced by enterovirus on RD cells.	./cache/cord-281837-knswbb2d.txt	./txt/cord-281837-knswbb2d.txt