id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-281552-zfjy3m3i	Alsaadi, Entedar A. J.	Identification of a Membrane Binding Peptide in the Envelope Protein of MHV Coronavirus	2020-09-22		.txt	text/plain	4781	205	49	Here, we test E-derived peptides for membrane binding activity in vitro and confirm those identified as positive in the context of the full length protein expressed in two different cell types. Relative densitometry of the HSP and LSP bands revealed significant differences among the mutants with regard to their localisation to the different membrane fractions of E expressing insect cells ( Figure 5B ) and confirmed a role for the amphipathic MHV CoV E 50-64 peptide in membrane interaction. An amphipathic helix, EPTM, detected in the post-TM region of E, was suggested by bioinformatics analysis and assessed for direct membrane interaction in vitro by binding to GUVs. For comparison, the predicted E protein TM domain, ETM, and an established membrane active peptide from the influenza M2 protein were also included. Following expression of the complete E protein with mutations in the same identified peptide, altered membrane binding in two distinct cell types, mammalian and insect, was apparent.	./cache/cord-281552-zfjy3m3i.txt	./txt/cord-281552-zfjy3m3i.txt