id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-253282-zwl0safn	Plant, Ewan P.	Altering SARS Coronavirus Frameshift Efficiency Affects Genomic and Subgenomic RNA Production	2013-01-18		.txt	text/plain	5007	266	55	In previous studies, differences in the amount of genomic and subgenomic RNA produced by coronaviruses with mutations in the programmed ribosomal frameshift signal of ORF1a/b were observed. Here, analyses using synonymous protein coding mutations demonstrate that the region of the genome that harbors the frameshift signal affects the regulation of genomic and subgenomic RNA production without altering protein sequence. Here we describe deletion and mutagenesis experiments with a dual luciferase reporter to show that the effect the sequence between stems 1 and 2 has on frameshifting efficiency is due to structural changes those mutations cause in the pseudoknot. Similar to previously described viruses containing mutations in the slippery site of the frameshift signal [7] , here we show that mutations to the SARS-CoV frameshift stimulating mRNA pseudoknot can also affect the production of viral genomic RNA.	./cache/cord-253282-zwl0safn.txt	./txt/cord-253282-zwl0safn.txt