id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-252142-aqwlcs9g	Uematsu, Jun	Legume Lectins Inhibit Human Parainfluenza Virus Type 2 Infection by Interfering with the Entr	2012-06-29		.txt	text/plain	3037	167	55	Three lectins with different sugar binding specificities were investigated for anti-viral activity against human parainfluenza virus type 2 (hPIV-2). The lectins, concanavalin A (Con A), lens culinaris agglutinin (LCA) and peanut agglutinin (PNA), inhibited cell fusion and hemadsorption induced by hPIV-2. Using a recombinant green fluorescence protein-expressing hPIV-2, without matrix protein (rghPIV-2ΔM), it was found that virus entry into the cells was not completely prevented. The inhibitory effect of lectins on hPIV-2 entry into the cells and replication in the cells was analyzed using a recombinant green fluorescence protein-expressing hPIV-2 without matrix (M) protein (rghPIV-2ΔM) [9] . RNA was prepared from the lectin-treated infected cells at four days post infection, and virus-synthesized RNA was analyzed using hPIV-2 specific primers by polymerase chain reaction (PCR). These results indicated that the lectins bound to the cell surface receptors, and largely prevented the binding of hPIV-2 to the cells non-specifically.	./cache/cord-252142-aqwlcs9g.txt	./txt/cord-252142-aqwlcs9g.txt