id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-013178-li1x1m25	Hung, Ling-Chu	The Monoclonal Antibody Recognized the Open Reading Frame Protein in Porcine Circovirus Type 2-Infected Peripheral Blood Mononuclear Cells	2020-08-29		.txt	text/plain	9764	534	59	title: The Monoclonal Antibody Recognized the Open Reading Frame Protein in Porcine Circovirus Type 2-Infected Peripheral Blood Mononuclear Cells The purpose of this study in the context of the open reading frame 3 (ORF3) protein of porcine circovirus type 2 (PCV2) was especially its location and its relation to the capsid protein and the apoptosis protein in PCV2-infected porcine peripheral blood mononuclear cells (PBMCs). The mAb 7D3 binds to the ORF3 peptide (residues 35–66) and the native ORF3 protein in PCV2-infected PBMCs, as shown by immunofluorescence assay (IFA). Overall, this study provides a blueprint to explore the ORF3 protein in PCV2-infected PBMCs. The Porcine circovirus (PCV) is a small virus and contains closed circular single-stranded DNA [1] . For these purposes, this study used the commercial capsid antigen-ELISA and homemade ORF3 protein-ELISA (anti-N1 polyclonal antibodies and mAb 7D3 based) to detect viral proteins in pig blood.	./cache/cord-013178-li1x1m25.txt	./txt/cord-013178-li1x1m25.txt