id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003807-e2txo10z	Ke, Fei	Ranaviruses Bind Cells from Different Species through Interaction with Heparan Sulfate	2019-06-29		.txt	text/plain	5544	300	54	Here, viral cell-binding proteins and their cognate cellular receptors were investigated using two ranaviruses, Andrias davidianus ranavirus (ADRV) and Rana grylio virus (RGV), and two different cell lines, Chinese giant salamander thymus cells (GSTC) and Epithelioma papulosum cyprinid (EPC) cells. Furthermore, recombinant viral envelope proteins ADRV-58L and RGV-53R bound heparin-Sepharose beads implying the potential that cell surface HS is involved in the initial interaction between ranaviruses and susceptible host cells. Rana grylio virus (RGV) and Andrias davidianus ranavirus (ADRV) are ranaviruses isolated from diseased pig frogs R. Furthermore, since ranavirus viral envelope proteins [38] [39] [40] [45] [46] [47] [48] are likely involved in the initial interaction between virus and host, we examined the ability of RGV-53R and its ADRV homolog, ADVR-58L, to bind heparin-Sepharose beads. The effect of heparin on virus infection was tested by monitoring viral plaque formation following incubation of ADRV and RGV in the presence of increasing concentrations of heparin.	./cache/cord-003807-e2txo10z.txt	./txt/cord-003807-e2txo10z.txt