id author title date pages extension mime words sentences flesch summary cache txt cord-322005-70snojec Yao, Pingping Isolation and Growth Characteristics of SARS-CoV-2 in Vero Cell 2020-06-19 .txt text/plain 890 71 65 title: Isolation and Growth Characteristics of SARS-CoV-2 in Vero Cell In a recent report, 149 mutations were found among 103 sequenced isolates of SARS-CoV-2 . To investigate whether our viruses show mutations different from other reported SARS-CoV-2, all the seven isolates were sequenced at the 3rd passage and the sequences were aligned with coding sequence (CDS) of SARS-CoV-2 Wuhan-Hu-1 (NC_045512). It is interesting to note that these seven patients infected with SARS-CoV-2 have high viral load in the early stage of clinical sign, which is consistent with previous reports (Kim et al. A The cytopathic effect was observed in Vero cells infected with the isolated viruses at 5 dpi. In conclusion, seven SARS-CoV-2 strains were isolated, sequenced and characterized in Vero cells, and a deletion mutation was identified after short passage in Vero cells. Viral load kinetics of SARS-CoV-2 infection in first two patients in Korea SARS-CoV-2 viral load in upper respiratory specimens of infected patients ./cache/cord-322005-70snojec.txt ./txt/cord-322005-70snojec.txt