id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-014946-l3vwb2v7	Tian, Hong	Genetic variation analyses of nsp2 gene of PRRSV in Ningxia Hui Autonomous Region of China	2009-05-28		.txt	text/plain	634	30	60	title: Genetic variation analyses of nsp2 gene of PRRSV in Ningxia Hui Autonomous Region of China To gain a better understanding of the genetic diversity and evolution of PRRSV in the Ningxia Hui Nationality Autonomous Region (Ningxia) of China, the nsp2 genes from a series of PRRSV strains collected from the region in 2007 were partially sequenced. Comparison of the nucleotide sequence with ch-1a indicated that nsp2 genes of seventeen Ningxia isolates (NX strain) have deletions of 87 nucleotides. The nsp2 genes of the seventeen isolates had 74.9%–100% nucleotide sequence identities with each other. The amplified PCR products were analyzed by agarose gel electrophoresis and purified using the QIAquick gel extraction kit (Qiagen) per the manufacturer's protocol. All nucleotide sequences were obtained from clinical sample RNA by direct sequencing of PCR products. The nsp2 genes of seventeen isolates had 74.9%-100% nucleotide sequence identity with each other (Fig.3) .	./cache/cord-014946-l3vwb2v7.txt	./txt/cord-014946-l3vwb2v7.txt