id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-014923-gfiqjgxg	Zhang, Yi	The functional motif of SARS-CoV S protein involved in the interaction with ACE2	2009-09-16		.txt	text/plain	1846	115	61	It has been established that the S protein in this pathogen plays an important rule in the adsorption and penetration of SARS-CoV into the host cell by interaction with the ACE2 receptor. To determinant which functional motif of the S protein was involved in the interaction with ACE2, seven truncated S proteins deleted from the N or C terminal were obtained by an E.coli expression system and purified by column chromatography to homogeneity. The adsorption were quantified by ELISA, and the results indicated that amino acids from 388 to 496 of the S protein was responsible for the interaction with the ACE2 receptor, and the interaction could be completely disrupted by an antibody specific to these amino acids. Our results showed that S1-1, S1-2, S1-3, S1-5 and S1-7 can interact with purified ACE2 protein specifically, but S1-4 and S1-6 cannot interact with this receptor. Inactivated SARS-CoV vaccine elicits high titers of spike protein specific antibodies that block receptor binding and virus entry	./cache/cord-014923-gfiqjgxg.txt	./txt/cord-014923-gfiqjgxg.txt