id sid tid token lemma pos cord-261579-f0prnpsu 1 1 key key NNP cord-261579-f0prnpsu 1 2 : : : cord-261579-f0prnpsu 1 3 cord-261579-f0prnpsu cord-261579-f0prnpsu VBN cord-261579-f0prnpsu 1 4 authors author NNS cord-261579-f0prnpsu 1 5 : : : cord-261579-f0prnpsu 1 6 Eichler Eichler NNP cord-261579-f0prnpsu 1 7 , , , cord-261579-f0prnpsu 1 8 Robert Robert NNP cord-261579-f0prnpsu 1 9 ; ; : cord-261579-f0prnpsu 1 10 Lenz Lenz NNP cord-261579-f0prnpsu 1 11 , , , cord-261579-f0prnpsu 1 12 Oliver Oliver NNP cord-261579-f0prnpsu 1 13 ; ; : cord-261579-f0prnpsu 1 14 Garten Garten NNP cord-261579-f0prnpsu 1 15 , , , cord-261579-f0prnpsu 1 16 Wolfgang Wolfgang NNP cord-261579-f0prnpsu 1 17 ; ; : cord-261579-f0prnpsu 1 18 Strecker Strecker NNP cord-261579-f0prnpsu 1 19 , , , cord-261579-f0prnpsu 1 20 Thomas Thomas NNP cord-261579-f0prnpsu 1 21 title title NN cord-261579-f0prnpsu 1 22 : : : cord-261579-f0prnpsu 1 23 The the DT cord-261579-f0prnpsu 1 24 role role NN cord-261579-f0prnpsu 1 25 of of IN cord-261579-f0prnpsu 1 26 single single JJ cord-261579-f0prnpsu 1 27 N n CD cord-261579-f0prnpsu 1 28 - - HYPH cord-261579-f0prnpsu 1 29 glycans glycan NNS cord-261579-f0prnpsu 1 30 in in IN cord-261579-f0prnpsu 1 31 proteolytic proteolytic JJ cord-261579-f0prnpsu 1 32 processing processing NN cord-261579-f0prnpsu 1 33 and and CC cord-261579-f0prnpsu 1 34 cell cell NN cord-261579-f0prnpsu 1 35 surface surface NN cord-261579-f0prnpsu 1 36 transport transport NN cord-261579-f0prnpsu 1 37 of of IN cord-261579-f0prnpsu 1 38 the the DT cord-261579-f0prnpsu 1 39 Lassa Lassa NNP cord-261579-f0prnpsu 1 40 virus virus NN cord-261579-f0prnpsu 1 41 glycoprotein glycoprotein NN cord-261579-f0prnpsu 1 42 GP gp NN cord-261579-f0prnpsu 1 43 - - HYPH cord-261579-f0prnpsu 1 44 C C NNP cord-261579-f0prnpsu 1 45 date date NN cord-261579-f0prnpsu 1 46 : : : cord-261579-f0prnpsu 1 47 2006 2006 CD cord-261579-f0prnpsu 1 48 - - HYPH cord-261579-f0prnpsu 1 49 05 05 CD cord-261579-f0prnpsu 1 50 - - HYPH cord-261579-f0prnpsu 1 51 31 31 CD cord-261579-f0prnpsu 1 52 journal journal NN cord-261579-f0prnpsu 1 53 : : : cord-261579-f0prnpsu 2 1 Virol Virol NNP cord-261579-f0prnpsu 2 2 J J NNP cord-261579-f0prnpsu 2 3 DOI DOI NNP cord-261579-f0prnpsu 2 4 : : : cord-261579-f0prnpsu 2 5 10.1186/1743 10.1186/1743 CD cord-261579-f0prnpsu 2 6 - - HYPH cord-261579-f0prnpsu 2 7 422x-3 422x-3 CD cord-261579-f0prnpsu 2 8 - - SYM cord-261579-f0prnpsu 2 9 41 41 CD cord-261579-f0prnpsu 3 1 sha sha NNP cord-261579-f0prnpsu 3 2 : : : cord-261579-f0prnpsu 3 3 48d2cdd1975a60aa6d7ab15b51b523b10529b038 48d2cdd1975a60aa6d7ab15b51b523b10529b038 CD cord-261579-f0prnpsu 3 4 doc_id doc_id CD cord-261579-f0prnpsu 3 5 : : : cord-261579-f0prnpsu 4 1 261579 261579 CD cord-261579-f0prnpsu 4 2 cord_uid cord_uid NNS cord-261579-f0prnpsu 4 3 : : : cord-261579-f0prnpsu 5 1 f0prnpsu f0prnpsu NNP cord-261579-f0prnpsu 5 2 Lassa Lassa NNP cord-261579-f0prnpsu 5 3 virus virus NN cord-261579-f0prnpsu 5 4 glycoprotein glycoprotein NN cord-261579-f0prnpsu 5 5 is be VBZ cord-261579-f0prnpsu 5 6 synthesised synthesise VBN cord-261579-f0prnpsu 5 7 as as IN cord-261579-f0prnpsu 5 8 a a DT cord-261579-f0prnpsu 5 9 precursor precursor NN cord-261579-f0prnpsu 5 10 ( ( -LRB- cord-261579-f0prnpsu 5 11 preGP preGP NNP cord-261579-f0prnpsu 5 12 - - HYPH cord-261579-f0prnpsu 5 13 C C NNP cord-261579-f0prnpsu 5 14 ) ) -RRB- cord-261579-f0prnpsu 5 15 into into IN cord-261579-f0prnpsu 5 16 the the DT cord-261579-f0prnpsu 5 17 lumen lumen NN cord-261579-f0prnpsu 5 18 of of IN cord-261579-f0prnpsu 5 19 the the DT cord-261579-f0prnpsu 5 20 endoplasmic endoplasmic NN cord-261579-f0prnpsu 5 21 reticulum reticulum NN cord-261579-f0prnpsu 5 22 . . . cord-261579-f0prnpsu 6 1 After after IN cord-261579-f0prnpsu 6 2 cotranslational cotranslational JJ cord-261579-f0prnpsu 6 3 cleavage cleavage NN cord-261579-f0prnpsu 6 4 of of IN cord-261579-f0prnpsu 6 5 the the DT cord-261579-f0prnpsu 6 6 signal signal NN cord-261579-f0prnpsu 6 7 peptide peptide NN cord-261579-f0prnpsu 6 8 , , , cord-261579-f0prnpsu 6 9 the the DT cord-261579-f0prnpsu 6 10 immature immature JJ cord-261579-f0prnpsu 6 11 GP GP NNP cord-261579-f0prnpsu 6 12 - - HYPH cord-261579-f0prnpsu 6 13 C C NNP cord-261579-f0prnpsu 6 14 is be VBZ cord-261579-f0prnpsu 6 15 posttranslationally posttranslationally RB cord-261579-f0prnpsu 6 16 processed process VBN cord-261579-f0prnpsu 6 17 into into IN cord-261579-f0prnpsu 6 18 the the DT cord-261579-f0prnpsu 6 19 N n CD cord-261579-f0prnpsu 6 20 - - HYPH cord-261579-f0prnpsu 6 21 terminal terminal NN cord-261579-f0prnpsu 6 22 subunit subunit NN cord-261579-f0prnpsu 6 23 GP-1 GP-1 NNP cord-261579-f0prnpsu 6 24 and and CC cord-261579-f0prnpsu 6 25 the the DT cord-261579-f0prnpsu 6 26 C C NNP cord-261579-f0prnpsu 6 27 - - HYPH cord-261579-f0prnpsu 6 28 terminal terminal NN cord-261579-f0prnpsu 6 29 subunit subunit NN cord-261579-f0prnpsu 6 30 GP-2 GP-2 NNP cord-261579-f0prnpsu 6 31 by by IN cord-261579-f0prnpsu 6 32 the the DT cord-261579-f0prnpsu 6 33 host host NN cord-261579-f0prnpsu 6 34 cell cell NN cord-261579-f0prnpsu 6 35 subtilase subtilase NNP cord-261579-f0prnpsu 6 36 SKI-1 SKI-1 NNP cord-261579-f0prnpsu 6 37 / / SYM cord-261579-f0prnpsu 6 38 S1P. S1P. NNP cord-261579-f0prnpsu 7 1 The the DT cord-261579-f0prnpsu 7 2 glycoprotein glycoprotein NN cord-261579-f0prnpsu 7 3 precursor precursor NN cord-261579-f0prnpsu 7 4 contains contain VBZ cord-261579-f0prnpsu 7 5 eleven eleven CD cord-261579-f0prnpsu 7 6 potential potential JJ cord-261579-f0prnpsu 7 7 N n CD cord-261579-f0prnpsu 7 8 - - HYPH cord-261579-f0prnpsu 7 9 glycosylation glycosylation NN cord-261579-f0prnpsu 7 10 sites site NNS cord-261579-f0prnpsu 7 11 . . . cord-261579-f0prnpsu 8 1 In in IN cord-261579-f0prnpsu 8 2 this this DT cord-261579-f0prnpsu 8 3 report report NN cord-261579-f0prnpsu 8 4 , , , cord-261579-f0prnpsu 8 5 we -PRON- PRP cord-261579-f0prnpsu 8 6 investigated investigate VBD cord-261579-f0prnpsu 8 7 the the DT cord-261579-f0prnpsu 8 8 effect effect NN cord-261579-f0prnpsu 8 9 of of IN cord-261579-f0prnpsu 8 10 each each DT cord-261579-f0prnpsu 8 11 N n NN cord-261579-f0prnpsu 8 12 - - HYPH cord-261579-f0prnpsu 8 13 glycan glycan NN cord-261579-f0prnpsu 8 14 on on IN cord-261579-f0prnpsu 8 15 proteolytic proteolytic JJ cord-261579-f0prnpsu 8 16 cleavage cleavage NN cord-261579-f0prnpsu 8 17 and and CC cord-261579-f0prnpsu 8 18 cell cell NN cord-261579-f0prnpsu 8 19 surface surface NN cord-261579-f0prnpsu 8 20 transport transport NN cord-261579-f0prnpsu 8 21 by by IN cord-261579-f0prnpsu 8 22 disrupting disrupt VBG cord-261579-f0prnpsu 8 23 the the DT cord-261579-f0prnpsu 8 24 consensus consensus NN cord-261579-f0prnpsu 8 25 sequences sequence NNS cord-261579-f0prnpsu 8 26 of of IN cord-261579-f0prnpsu 8 27 eleven eleven CD cord-261579-f0prnpsu 8 28 potential potential JJ cord-261579-f0prnpsu 8 29 N n CD cord-261579-f0prnpsu 8 30 - - HYPH cord-261579-f0prnpsu 8 31 glycan glycan NN cord-261579-f0prnpsu 8 32 attachment attachment NN cord-261579-f0prnpsu 8 33 sites site NNS cord-261579-f0prnpsu 8 34 individually individually RB cord-261579-f0prnpsu 8 35 . . . cord-261579-f0prnpsu 9 1 Five five CD cord-261579-f0prnpsu 9 2 glycoprotein glycoprotein NN cord-261579-f0prnpsu 9 3 mutants mutant NNS cord-261579-f0prnpsu 9 4 with with IN cord-261579-f0prnpsu 9 5 disrupted disrupt VBN cord-261579-f0prnpsu 9 6 N n NN cord-261579-f0prnpsu 9 7 - - HYPH cord-261579-f0prnpsu 9 8 glycosylation glycosylation NN cord-261579-f0prnpsu 9 9 sites site NNS cord-261579-f0prnpsu 9 10 were be VBD cord-261579-f0prnpsu 9 11 still still RB cord-261579-f0prnpsu 9 12 proteolytically proteolytically RB cord-261579-f0prnpsu 9 13 processed process VBN cord-261579-f0prnpsu 9 14 , , , cord-261579-f0prnpsu 9 15 whereas whereas IN cord-261579-f0prnpsu 9 16 the the DT cord-261579-f0prnpsu 9 17 remaining remain VBG cord-261579-f0prnpsu 9 18 N n CD cord-261579-f0prnpsu 9 19 - - HYPH cord-261579-f0prnpsu 9 20 glycosylation glycosylation NN cord-261579-f0prnpsu 9 21 sites site NNS cord-261579-f0prnpsu 9 22 are be VBP cord-261579-f0prnpsu 9 23 necessary necessary JJ cord-261579-f0prnpsu 9 24 for for IN cord-261579-f0prnpsu 9 25 GP gp NN cord-261579-f0prnpsu 9 26 - - HYPH cord-261579-f0prnpsu 9 27 C c NN cord-261579-f0prnpsu 9 28 cleavage cleavage NN cord-261579-f0prnpsu 9 29 . . . cord-261579-f0prnpsu 10 1 Despite despite IN cord-261579-f0prnpsu 10 2 the the DT cord-261579-f0prnpsu 10 3 lack lack NN cord-261579-f0prnpsu 10 4 of of IN cord-261579-f0prnpsu 10 5 proteolytic proteolytic JJ cord-261579-f0prnpsu 10 6 processing processing NN cord-261579-f0prnpsu 10 7 , , , cord-261579-f0prnpsu 10 8 all all DT cord-261579-f0prnpsu 10 9 cleavage cleavage NN cord-261579-f0prnpsu 10 10 - - HYPH cord-261579-f0prnpsu 10 11 defective defective JJ cord-261579-f0prnpsu 10 12 mutants mutant NNS cord-261579-f0prnpsu 10 13 were be VBD cord-261579-f0prnpsu 10 14 transported transport VBN cord-261579-f0prnpsu 10 15 to to IN cord-261579-f0prnpsu 10 16 the the DT cord-261579-f0prnpsu 10 17 cell cell NN cord-261579-f0prnpsu 10 18 surface surface NN cord-261579-f0prnpsu 10 19 and and CC cord-261579-f0prnpsu 10 20 remained remain VBD cord-261579-f0prnpsu 10 21 completely completely RB cord-261579-f0prnpsu 10 22 endo endo NN cord-261579-f0prnpsu 10 23 H h NN cord-261579-f0prnpsu 10 24 - - HYPH cord-261579-f0prnpsu 10 25 sensitive sensitive JJ cord-261579-f0prnpsu 10 26 . . . cord-261579-f0prnpsu 11 1 The the DT cord-261579-f0prnpsu 11 2 findings finding NNS cord-261579-f0prnpsu 11 3 indicate indicate VBP cord-261579-f0prnpsu 11 4 that that IN cord-261579-f0prnpsu 11 5 N n NN cord-261579-f0prnpsu 11 6 - - HYPH cord-261579-f0prnpsu 11 7 glycans glycan NNS cord-261579-f0prnpsu 11 8 are be VBP cord-261579-f0prnpsu 11 9 needed need VBN cord-261579-f0prnpsu 11 10 for for IN cord-261579-f0prnpsu 11 11 correct correct JJ cord-261579-f0prnpsu 11 12 conformation conformation NN cord-261579-f0prnpsu 11 13 of of IN cord-261579-f0prnpsu 11 14 GP GP NNP cord-261579-f0prnpsu 11 15 - - HYPH cord-261579-f0prnpsu 11 16 C C NNP cord-261579-f0prnpsu 11 17 in in IN cord-261579-f0prnpsu 11 18 order order NN cord-261579-f0prnpsu 11 19 to to TO cord-261579-f0prnpsu 11 20 be be VB cord-261579-f0prnpsu 11 21 cleaved cleave VBN cord-261579-f0prnpsu 11 22 by by IN cord-261579-f0prnpsu 11 23 SKI-1 SKI-1 NNP cord-261579-f0prnpsu 11 24 / / SYM cord-261579-f0prnpsu 11 25 S1P. S1P. NNP cord-261579-f0prnpsu 11 26 Lassa Lassa NNP cord-261579-f0prnpsu 11 27 virus virus NN cord-261579-f0prnpsu 11 28 belongs belong VBZ cord-261579-f0prnpsu 11 29 to to IN cord-261579-f0prnpsu 11 30 the the DT cord-261579-f0prnpsu 11 31 family family NN cord-261579-f0prnpsu 11 32 of of IN cord-261579-f0prnpsu 11 33 Arenaviridae Arenaviridae NNP cord-261579-f0prnpsu 11 34 which which WDT cord-261579-f0prnpsu 11 35 includes include VBZ cord-261579-f0prnpsu 11 36 other other JJ cord-261579-f0prnpsu 11 37 important important JJ cord-261579-f0prnpsu 11 38 human human JJ cord-261579-f0prnpsu 11 39 pathogens pathogen NNS cord-261579-f0prnpsu 11 40 like like IN cord-261579-f0prnpsu 11 41 Machupo Machupo NNP cord-261579-f0prnpsu 11 42 virus virus NN cord-261579-f0prnpsu 11 43 , , , cord-261579-f0prnpsu 11 44 Junin Junin NNP cord-261579-f0prnpsu 11 45 virus virus NN cord-261579-f0prnpsu 11 46 , , , cord-261579-f0prnpsu 11 47 Guanarito Guanarito NNP cord-261579-f0prnpsu 11 48 virus virus NN cord-261579-f0prnpsu 11 49 and and CC cord-261579-f0prnpsu 11 50 Sabia Sabia NNP cord-261579-f0prnpsu 11 51 virus virus NN cord-261579-f0prnpsu 11 52 as as RB cord-261579-f0prnpsu 11 53 well well RB cord-261579-f0prnpsu 11 54 as as IN cord-261579-f0prnpsu 11 55 the the DT cord-261579-f0prnpsu 11 56 prototype prototype NN cord-261579-f0prnpsu 11 57 of of IN cord-261579-f0prnpsu 11 58 this this DT cord-261579-f0prnpsu 11 59 family family NN cord-261579-f0prnpsu 11 60 , , , cord-261579-f0prnpsu 11 61 Lymphocytic Lymphocytic NNP cord-261579-f0prnpsu 11 62 Choriomeningitis Choriomeningitis NNP cord-261579-f0prnpsu 11 63 virus virus NN cord-261579-f0prnpsu 11 64 ( ( -LRB- cord-261579-f0prnpsu 11 65 LCMV LCMV NNP cord-261579-f0prnpsu 11 66 ) ) -RRB- cord-261579-f0prnpsu 11 67 . . . cord-261579-f0prnpsu 12 1 Lassa Lassa NNP cord-261579-f0prnpsu 12 2 virus virus NN cord-261579-f0prnpsu 12 3 is be VBZ cord-261579-f0prnpsu 12 4 endemic endemic JJ cord-261579-f0prnpsu 12 5 in in IN cord-261579-f0prnpsu 12 6 certain certain JJ cord-261579-f0prnpsu 12 7 parts part NNS cord-261579-f0prnpsu 12 8 of of IN cord-261579-f0prnpsu 12 9 West West NNP cord-261579-f0prnpsu 12 10 Africa Africa NNP cord-261579-f0prnpsu 12 11 and and CC cord-261579-f0prnpsu 12 12 causes cause VBZ cord-261579-f0prnpsu 12 13 an an DT cord-261579-f0prnpsu 12 14 estimated estimate VBN cord-261579-f0prnpsu 12 15 150 150 CD cord-261579-f0prnpsu 12 16 000 000 CD cord-261579-f0prnpsu 12 17 clinical clinical JJ cord-261579-f0prnpsu 12 18 cases case NNS cord-261579-f0prnpsu 12 19 of of IN cord-261579-f0prnpsu 12 20 a a DT cord-261579-f0prnpsu 12 21 systemic systemic JJ cord-261579-f0prnpsu 12 22 viral viral JJ cord-261579-f0prnpsu 12 23 illness illness NN cord-261579-f0prnpsu 12 24 per per IN cord-261579-f0prnpsu 12 25 year year NN cord-261579-f0prnpsu 12 26 , , , cord-261579-f0prnpsu 12 27 with with IN cord-261579-f0prnpsu 12 28 a a DT cord-261579-f0prnpsu 12 29 mortality mortality NN cord-261579-f0prnpsu 12 30 rate rate NN cord-261579-f0prnpsu 12 31 of of IN cord-261579-f0prnpsu 12 32 10 10 CD cord-261579-f0prnpsu 12 33 - - SYM cord-261579-f0prnpsu 12 34 15 15 CD cord-261579-f0prnpsu 12 35 % % NN cord-261579-f0prnpsu 12 36 [ [ -LRB- cord-261579-f0prnpsu 12 37 1 1 CD cord-261579-f0prnpsu 12 38 ] ] -RRB- cord-261579-f0prnpsu 12 39 . . . cord-261579-f0prnpsu 13 1 Lassa Lassa NNP cord-261579-f0prnpsu 13 2 fever fever NN cord-261579-f0prnpsu 13 3 is be VBZ cord-261579-f0prnpsu 13 4 not not RB cord-261579-f0prnpsu 13 5 only only RB cord-261579-f0prnpsu 13 6 a a DT cord-261579-f0prnpsu 13 7 public public JJ cord-261579-f0prnpsu 13 8 health health NN cord-261579-f0prnpsu 13 9 concern concern NN cord-261579-f0prnpsu 13 10 in in IN cord-261579-f0prnpsu 13 11 endemic endemic JJ cord-261579-f0prnpsu 13 12 areas area NNS cord-261579-f0prnpsu 13 13 , , , cord-261579-f0prnpsu 13 14 it -PRON- PRP cord-261579-f0prnpsu 13 15 is be VBZ cord-261579-f0prnpsu 13 16 also also RB cord-261579-f0prnpsu 13 17 sporadically sporadically RB cord-261579-f0prnpsu 13 18 exported export VBN cord-261579-f0prnpsu 13 19 to to IN cord-261579-f0prnpsu 13 20 other other JJ cord-261579-f0prnpsu 13 21 parts part NNS cord-261579-f0prnpsu 13 22 of of IN cord-261579-f0prnpsu 13 23 the the DT cord-261579-f0prnpsu 13 24 world world NN cord-261579-f0prnpsu 13 25 [ [ -LRB- cord-261579-f0prnpsu 13 26 2 2 CD cord-261579-f0prnpsu 13 27 ] ] -RRB- cord-261579-f0prnpsu 13 28 . . . cord-261579-f0prnpsu 14 1 Lassa Lassa NNP cord-261579-f0prnpsu 14 2 virus virus NN cord-261579-f0prnpsu 14 3 is be VBZ cord-261579-f0prnpsu 14 4 an an DT cord-261579-f0prnpsu 14 5 enveloped envelop VBN cord-261579-f0prnpsu 14 6 virus virus NN cord-261579-f0prnpsu 14 7 with with IN cord-261579-f0prnpsu 14 8 a a DT cord-261579-f0prnpsu 14 9 bi bi JJ cord-261579-f0prnpsu 14 10 - - JJ cord-261579-f0prnpsu 14 11 segmented segmented JJ cord-261579-f0prnpsu 14 12 RNA RNA NNP cord-261579-f0prnpsu 14 13 genome genome NN cord-261579-f0prnpsu 14 14 which which WDT cord-261579-f0prnpsu 14 15 encodes encode VBZ cord-261579-f0prnpsu 14 16 four four CD cord-261579-f0prnpsu 14 17 viral viral JJ cord-261579-f0prnpsu 14 18 proteins protein NNS cord-261579-f0prnpsu 14 19 in in IN cord-261579-f0prnpsu 14 20 an an DT cord-261579-f0prnpsu 14 21 ambisense ambisense NN cord-261579-f0prnpsu 14 22 coding coding NN cord-261579-f0prnpsu 14 23 strategy strategy NN cord-261579-f0prnpsu 14 24 : : : cord-261579-f0prnpsu 14 25 the the DT cord-261579-f0prnpsu 14 26 glycoprotein glycoprotein NN cord-261579-f0prnpsu 14 27 precursor precursor NN cord-261579-f0prnpsu 14 28 preGP preGP NNP cord-261579-f0prnpsu 14 29 - - HYPH cord-261579-f0prnpsu 14 30 C C NNP cord-261579-f0prnpsu 14 31 , , , cord-261579-f0prnpsu 14 32 a a DT cord-261579-f0prnpsu 14 33 nucleoprotein nucleoprotein NNP cord-261579-f0prnpsu 14 34 ( ( -LRB- cord-261579-f0prnpsu 14 35 NP NP NNP cord-261579-f0prnpsu 14 36 ) ) -RRB- cord-261579-f0prnpsu 14 37 , , , cord-261579-f0prnpsu 14 38 the the DT cord-261579-f0prnpsu 14 39 viral viral JJ cord-261579-f0prnpsu 14 40 polymerase polymerase NN cord-261579-f0prnpsu 14 41 L l NN cord-261579-f0prnpsu 14 42 and and CC cord-261579-f0prnpsu 14 43 the the DT cord-261579-f0prnpsu 14 44 matrix matrix NN cord-261579-f0prnpsu 14 45 protein protein NN cord-261579-f0prnpsu 14 46 Z Z NNP cord-261579-f0prnpsu 14 47 ( ( -LRB- cord-261579-f0prnpsu 14 48 for for IN cord-261579-f0prnpsu 14 49 review review NN cord-261579-f0prnpsu 14 50 see see VBP cord-261579-f0prnpsu 14 51 [ [ -LRB- cord-261579-f0prnpsu 14 52 3 3 CD cord-261579-f0prnpsu 14 53 ] ] -RRB- cord-261579-f0prnpsu 14 54 ) ) -RRB- cord-261579-f0prnpsu 14 55 . . . cord-261579-f0prnpsu 15 1 The the DT cord-261579-f0prnpsu 15 2 Lassa Lassa NNP cord-261579-f0prnpsu 15 3 virus virus NN cord-261579-f0prnpsu 15 4 glycoprotein glycoprotein NN cord-261579-f0prnpsu 15 5 is be VBZ cord-261579-f0prnpsu 15 6 translated translate VBN cord-261579-f0prnpsu 15 7 as as IN cord-261579-f0prnpsu 15 8 an an DT cord-261579-f0prnpsu 15 9 inactive inactive JJ cord-261579-f0prnpsu 15 10 precursor precursor NN cord-261579-f0prnpsu 15 11 ( ( -LRB- cord-261579-f0prnpsu 15 12 pre pre JJ cord-261579-f0prnpsu 15 13 - - JJ cord-261579-f0prnpsu 15 14 GP gp NN cord-261579-f0prnpsu 15 15 - - HYPH cord-261579-f0prnpsu 15 16 C C NNP cord-261579-f0prnpsu 15 17 ) ) -RRB- cord-261579-f0prnpsu 15 18 into into IN cord-261579-f0prnpsu 15 19 the the DT cord-261579-f0prnpsu 15 20 lumen lumen NN cord-261579-f0prnpsu 15 21 of of IN cord-261579-f0prnpsu 15 22 the the DT cord-261579-f0prnpsu 15 23 endoplasmic endoplasmic NN cord-261579-f0prnpsu 15 24 reticulum reticulum NN cord-261579-f0prnpsu 15 25 and and CC cord-261579-f0prnpsu 15 26 is be VBZ cord-261579-f0prnpsu 15 27 then then RB cord-261579-f0prnpsu 15 28 cotranslationally cotranslationally RB cord-261579-f0prnpsu 15 29 cleaved cleave VBN cord-261579-f0prnpsu 15 30 into into IN cord-261579-f0prnpsu 15 31 GP GP NNP cord-261579-f0prnpsu 15 32 - - HYPH cord-261579-f0prnpsu 15 33 C c NN cord-261579-f0prnpsu 15 34 and and CC cord-261579-f0prnpsu 15 35 a a DT cord-261579-f0prnpsu 15 36 stable stable JJ cord-261579-f0prnpsu 15 37 signal signal NN cord-261579-f0prnpsu 15 38 peptide peptide NN cord-261579-f0prnpsu 15 39 [ [ -LRB- cord-261579-f0prnpsu 15 40 4 4 CD cord-261579-f0prnpsu 15 41 ] ] -RRB- cord-261579-f0prnpsu 15 42 . . . cord-261579-f0prnpsu 16 1 The the DT cord-261579-f0prnpsu 16 2 latter latter JJ cord-261579-f0prnpsu 16 3 one one NN cord-261579-f0prnpsu 16 4 plays play VBZ cord-261579-f0prnpsu 16 5 an an DT cord-261579-f0prnpsu 16 6 important important JJ cord-261579-f0prnpsu 16 7 role role NN cord-261579-f0prnpsu 16 8 in in IN cord-261579-f0prnpsu 16 9 the the DT cord-261579-f0prnpsu 16 10 subsequent subsequent JJ cord-261579-f0prnpsu 16 11 posttranslational posttranslational JJ cord-261579-f0prnpsu 16 12 maturation maturation NN cord-261579-f0prnpsu 16 13 cleavage cleavage NN cord-261579-f0prnpsu 16 14 of of IN cord-261579-f0prnpsu 16 15 GP gp NN cord-261579-f0prnpsu 16 16 - - HYPH cord-261579-f0prnpsu 16 17 C C NNP cord-261579-f0prnpsu 16 18 into into IN cord-261579-f0prnpsu 16 19 its -PRON- PRP$ cord-261579-f0prnpsu 16 20 subunits subunit NNS cord-261579-f0prnpsu 16 21 GP1 gp1 NN cord-261579-f0prnpsu 16 22 and and CC cord-261579-f0prnpsu 16 23 GP2 GP2 NNP cord-261579-f0prnpsu 17 1 [ [ -LRB- cord-261579-f0prnpsu 17 2 5 5 CD cord-261579-f0prnpsu 17 3 , , , cord-261579-f0prnpsu 17 4 6 6 CD cord-261579-f0prnpsu 17 5 ] ] -RRB- cord-261579-f0prnpsu 17 6 . . . cord-261579-f0prnpsu 18 1 Lassa Lassa NNP cord-261579-f0prnpsu 18 2 virus virus NN cord-261579-f0prnpsu 18 3 GP GP NNP cord-261579-f0prnpsu 18 4 - - HYPH cord-261579-f0prnpsu 18 5 C C NNP cord-261579-f0prnpsu 18 6 is be VBZ cord-261579-f0prnpsu 18 7 cleaved cleave VBN cord-261579-f0prnpsu 18 8 at at IN cord-261579-f0prnpsu 18 9 the the DT cord-261579-f0prnpsu 18 10 C C NNP cord-261579-f0prnpsu 18 11 - - HYPH cord-261579-f0prnpsu 18 12 terminus terminus NN cord-261579-f0prnpsu 18 13 of of IN cord-261579-f0prnpsu 18 14 a a DT cord-261579-f0prnpsu 18 15 non non JJ cord-261579-f0prnpsu 18 16 - - JJ cord-261579-f0prnpsu 18 17 basic basic JJ cord-261579-f0prnpsu 18 18 amino amino NN cord-261579-f0prnpsu 18 19 acid acid NN cord-261579-f0prnpsu 18 20 residue residue NN cord-261579-f0prnpsu 18 21 of of IN cord-261579-f0prnpsu 18 22 the the DT cord-261579-f0prnpsu 18 23 cleavage cleavage NN cord-261579-f0prnpsu 18 24 motif motif NN cord-261579-f0prnpsu 18 25 R r NN cord-261579-f0prnpsu 18 26 - - HYPH cord-261579-f0prnpsu 18 27 R R NNP cord-261579-f0prnpsu 18 28 - - HYPH cord-261579-f0prnpsu 18 29 L L NNP cord-261579-f0prnpsu 18 30 - - HYPH cord-261579-f0prnpsu 18 31 L L NNP cord-261579-f0prnpsu 18 32 by by IN cord-261579-f0prnpsu 18 33 the the DT cord-261579-f0prnpsu 18 34 subtilase subtilase NN cord-261579-f0prnpsu 19 1 SKI-1/ ski-1/ NN cord-261579-f0prnpsu 20 1 S1P S1P NNP cord-261579-f0prnpsu 20 2 , , , cord-261579-f0prnpsu 20 3 which which WDT cord-261579-f0prnpsu 20 4 is be VBZ cord-261579-f0prnpsu 20 5 unusual unusual JJ cord-261579-f0prnpsu 20 6 for for IN cord-261579-f0prnpsu 20 7 fusiogenic fusiogenic JJ cord-261579-f0prnpsu 20 8 glycoproteins glycoprotein NNS cord-261579-f0prnpsu 20 9 of of IN cord-261579-f0prnpsu 20 10 enveloped envelop VBN cord-261579-f0prnpsu 20 11 viruses virus NNS cord-261579-f0prnpsu 20 12 [ [ -LRB- cord-261579-f0prnpsu 20 13 7 7 CD cord-261579-f0prnpsu 20 14 ] ] -RRB- cord-261579-f0prnpsu 20 15 . . . cord-261579-f0prnpsu 21 1 So so RB cord-261579-f0prnpsu 21 2 far far RB cord-261579-f0prnpsu 21 3 , , , cord-261579-f0prnpsu 21 4 only only RB cord-261579-f0prnpsu 21 5 viral viral JJ cord-261579-f0prnpsu 21 6 glycoproteins glycoprotein NNS cord-261579-f0prnpsu 21 7 of of IN cord-261579-f0prnpsu 21 8 the the DT cord-261579-f0prnpsu 21 9 arenavirus arenavirus NNP cord-261579-f0prnpsu 21 10 family family NN cord-261579-f0prnpsu 21 11 and and CC cord-261579-f0prnpsu 21 12 the the DT cord-261579-f0prnpsu 21 13 glycoprotein glycoprotein NN cord-261579-f0prnpsu 21 14 of of IN cord-261579-f0prnpsu 21 15 the the DT cord-261579-f0prnpsu 21 16 Crimean Crimean NNP cord-261579-f0prnpsu 21 17 - - HYPH cord-261579-f0prnpsu 21 18 Congo Congo NNP cord-261579-f0prnpsu 21 19 hemorrhagic hemorrhagic JJ cord-261579-f0prnpsu 21 20 fever fever NN cord-261579-f0prnpsu 21 21 virus virus NN cord-261579-f0prnpsu 21 22 belonging belong VBG cord-261579-f0prnpsu 21 23 to to IN cord-261579-f0prnpsu 21 24 the the DT cord-261579-f0prnpsu 21 25 family family NN cord-261579-f0prnpsu 21 26 of of IN cord-261579-f0prnpsu 21 27 Bunyaviridae Bunyaviridae NNP cord-261579-f0prnpsu 21 28 are be VBP cord-261579-f0prnpsu 21 29 known know VBN cord-261579-f0prnpsu 21 30 to to TO cord-261579-f0prnpsu 21 31 be be VB cord-261579-f0prnpsu 21 32 cleaved cleave VBN cord-261579-f0prnpsu 21 33 by by IN cord-261579-f0prnpsu 21 34 SKI-1/ SKI-1/ NNP cord-261579-f0prnpsu 21 35 S1P s1p NN cord-261579-f0prnpsu 21 36 , , , cord-261579-f0prnpsu 21 37 an an DT cord-261579-f0prnpsu 21 38 enzyme enzyme NN cord-261579-f0prnpsu 21 39 that that WDT cord-261579-f0prnpsu 21 40 normally normally RB cord-261579-f0prnpsu 21 41 plays play VBZ cord-261579-f0prnpsu 21 42 a a DT cord-261579-f0prnpsu 21 43 role role NN cord-261579-f0prnpsu 21 44 in in IN cord-261579-f0prnpsu 21 45 regulation regulation NN cord-261579-f0prnpsu 21 46 of of IN cord-261579-f0prnpsu 21 47 the the DT cord-261579-f0prnpsu 21 48 lipid lipid NN cord-261579-f0prnpsu 21 49 metabolism metabolism NN cord-261579-f0prnpsu 21 50 of of IN cord-261579-f0prnpsu 21 51 the the DT cord-261579-f0prnpsu 21 52 cell cell NN cord-261579-f0prnpsu 21 53 [ [ -LRB- cord-261579-f0prnpsu 21 54 6 6 CD cord-261579-f0prnpsu 21 55 , , , cord-261579-f0prnpsu 21 56 [ [ -LRB- cord-261579-f0prnpsu 21 57 8 8 CD cord-261579-f0prnpsu 21 58 ] ] -RRB- cord-261579-f0prnpsu 21 59 [ [ -LRB- cord-261579-f0prnpsu 21 60 9 9 CD cord-261579-f0prnpsu 21 61 ] ] -RRB- cord-261579-f0prnpsu 21 62 [ [ -LRB- cord-261579-f0prnpsu 21 63 10 10 CD cord-261579-f0prnpsu 21 64 ] ] -RRB- cord-261579-f0prnpsu 21 65 . . . cord-261579-f0prnpsu 22 1 Fusiogenic fusiogenic JJ cord-261579-f0prnpsu 22 2 glyc glyc NN cord-261579-f0prnpsu 22 3 - - HYPH cord-261579-f0prnpsu 22 4 oproteins oprotein NNS cord-261579-f0prnpsu 22 5 of of IN cord-261579-f0prnpsu 22 6 most most JJS cord-261579-f0prnpsu 22 7 other other JJ cord-261579-f0prnpsu 22 8 enveloped envelop VBN cord-261579-f0prnpsu 22 9 viruses virus NNS cord-261579-f0prnpsu 22 10 are be VBP cord-261579-f0prnpsu 22 11 proteolytically proteolytically RB cord-261579-f0prnpsu 22 12 cleaved cleave VBN cord-261579-f0prnpsu 22 13 C c NN cord-261579-f0prnpsu 22 14 - - HYPH cord-261579-f0prnpsu 22 15 terminally terminally RB cord-261579-f0prnpsu 22 16 of of IN cord-261579-f0prnpsu 22 17 a a DT cord-261579-f0prnpsu 22 18 single single JJ cord-261579-f0prnpsu 22 19 arginine arginine NN cord-261579-f0prnpsu 22 20 residue residue NN cord-261579-f0prnpsu 22 21 or or CC cord-261579-f0prnpsu 22 22 at at IN cord-261579-f0prnpsu 22 23 a a DT cord-261579-f0prnpsu 22 24 multibasic multibasic NN cord-261579-f0prnpsu 22 25 recognition recognition NN cord-261579-f0prnpsu 22 26 motif motif NN cord-261579-f0prnpsu 22 27 by by IN cord-261579-f0prnpsu 22 28 the the DT cord-261579-f0prnpsu 22 29 subtilase subtilase NN cord-261579-f0prnpsu 22 30 furin furin NN cord-261579-f0prnpsu 22 31 [ [ -LRB- cord-261579-f0prnpsu 22 32 11 11 CD cord-261579-f0prnpsu 22 33 ] ] -RRB- cord-261579-f0prnpsu 22 34 . . . cord-261579-f0prnpsu 23 1 N N NNS cord-261579-f0prnpsu 23 2 - - HYPH cord-261579-f0prnpsu 23 3 glycans glycan NNS cord-261579-f0prnpsu 23 4 play play VBP cord-261579-f0prnpsu 23 5 not not RB cord-261579-f0prnpsu 23 6 only only RB cord-261579-f0prnpsu 23 7 an an DT cord-261579-f0prnpsu 23 8 important important JJ cord-261579-f0prnpsu 23 9 role role NN cord-261579-f0prnpsu 23 10 in in IN cord-261579-f0prnpsu 23 11 folding folding NN cord-261579-f0prnpsu 23 12 and and CC cord-261579-f0prnpsu 23 13 intracellular intracellular JJ cord-261579-f0prnpsu 23 14 transport transport NN cord-261579-f0prnpsu 23 15 of of IN cord-261579-f0prnpsu 23 16 viral viral JJ cord-261579-f0prnpsu 23 17 glycoproteins glycoprotein NNS cord-261579-f0prnpsu 23 18 but but CC cord-261579-f0prnpsu 23 19 also also RB cord-261579-f0prnpsu 23 20 are be VBP cord-261579-f0prnpsu 23 21 known know VBN cord-261579-f0prnpsu 23 22 to to TO cord-261579-f0prnpsu 23 23 modulate modulate VB cord-261579-f0prnpsu 23 24 their -PRON- PRP$ cord-261579-f0prnpsu 23 25 antigenicity antigenicity NN cord-261579-f0prnpsu 23 26 and and CC cord-261579-f0prnpsu 23 27 activity activity NN cord-261579-f0prnpsu 23 28 [ [ -LRB- cord-261579-f0prnpsu 23 29 12 12 CD cord-261579-f0prnpsu 23 30 ] ] -RRB- cord-261579-f0prnpsu 23 31 [ [ -LRB- cord-261579-f0prnpsu 23 32 13 13 CD cord-261579-f0prnpsu 23 33 ] ] -RRB- cord-261579-f0prnpsu 23 34 [ [ -LRB- cord-261579-f0prnpsu 23 35 14 14 CD cord-261579-f0prnpsu 23 36 ] ] -RRB- cord-261579-f0prnpsu 23 37 [ [ -LRB- cord-261579-f0prnpsu 23 38 15 15 CD cord-261579-f0prnpsu 23 39 ] ] -RRB- cord-261579-f0prnpsu 23 40 [ [ -LRB- cord-261579-f0prnpsu 23 41 16 16 CD cord-261579-f0prnpsu 23 42 ] ] -RRB- cord-261579-f0prnpsu 23 43 [ [ -LRB- cord-261579-f0prnpsu 23 44 17 17 CD cord-261579-f0prnpsu 23 45 ] ] -RRB- cord-261579-f0prnpsu 23 46 [ [ -LRB- cord-261579-f0prnpsu 23 47 18 18 CD cord-261579-f0prnpsu 23 48 ] ] -RRB- cord-261579-f0prnpsu 23 49 [ [ -LRB- cord-261579-f0prnpsu 23 50 19 19 CD cord-261579-f0prnpsu 23 51 ] ] -RRB- cord-261579-f0prnpsu 23 52 [ [ -LRB- cord-261579-f0prnpsu 23 53 20 20 CD cord-261579-f0prnpsu 23 54 ] ] -RRB- cord-261579-f0prnpsu 23 55 [ [ -LRB- cord-261579-f0prnpsu 23 56 21 21 CD cord-261579-f0prnpsu 23 57 ] ] -RRB- cord-261579-f0prnpsu 23 58 . . . cord-261579-f0prnpsu 24 1 Glycoproteins glycoprotein NNS cord-261579-f0prnpsu 24 2 of of IN cord-261579-f0prnpsu 24 3 arenaviruses arenavirus NNS cord-261579-f0prnpsu 24 4 vary vary VBP cord-261579-f0prnpsu 24 5 considerably considerably RB cord-261579-f0prnpsu 24 6 in in IN cord-261579-f0prnpsu 24 7 number number NN cord-261579-f0prnpsu 24 8 and and CC cord-261579-f0prnpsu 24 9 position position NN cord-261579-f0prnpsu 24 10 of of IN cord-261579-f0prnpsu 24 11 potential potential JJ cord-261579-f0prnpsu 24 12 N n CD cord-261579-f0prnpsu 24 13 - - HYPH cord-261579-f0prnpsu 24 14 glycosylation glycosylation NN cord-261579-f0prnpsu 24 15 sites site NNS cord-261579-f0prnpsu 24 16 as as IN cord-261579-f0prnpsu 24 17 shown show VBN cord-261579-f0prnpsu 24 18 by by IN cord-261579-f0prnpsu 24 19 an an DT cord-261579-f0prnpsu 24 20 N n CD cord-261579-f0prnpsu 24 21 - - HYPH cord-261579-f0prnpsu 24 22 glycosylation glycosylation NN cord-261579-f0prnpsu 24 23 prediction prediction NN cord-261579-f0prnpsu 24 24 program program NN cord-261579-f0prnpsu 24 25 ( ( -LRB- cord-261579-f0prnpsu 24 26 Table Table NNP cord-261579-f0prnpsu 24 27 _SP cord-261579-f0prnpsu 24 28 1 1 CD cord-261579-f0prnpsu 24 29 ) ) -RRB- cord-261579-f0prnpsu 24 30 . . . cord-261579-f0prnpsu 25 1 The the DT cord-261579-f0prnpsu 25 2 Lassa Lassa NNP cord-261579-f0prnpsu 25 3 virus virus NN cord-261579-f0prnpsu 25 4 glycoprotein glycoprotein NN cord-261579-f0prnpsu 25 5 GP GP NNP cord-261579-f0prnpsu 25 6 - - HYPH cord-261579-f0prnpsu 25 7 C C NNP cord-261579-f0prnpsu 25 8 contains contain VBZ cord-261579-f0prnpsu 25 9 eleven eleven CD cord-261579-f0prnpsu 25 10 potential potential JJ cord-261579-f0prnpsu 25 11 N n CD cord-261579-f0prnpsu 25 12 - - HYPH cord-261579-f0prnpsu 25 13 glycosylation glycosylation NN cord-261579-f0prnpsu 25 14 consensus consensus NN cord-261579-f0prnpsu 25 15 sites site NNS cord-261579-f0prnpsu 25 16 ( ( -LRB- cord-261579-f0prnpsu 25 17 N N NNP cord-261579-f0prnpsu 25 18 - - HYPH cord-261579-f0prnpsu 25 19 X X NNP cord-261579-f0prnpsu 25 20 - - HYPH cord-261579-f0prnpsu 25 21 S S NNP cord-261579-f0prnpsu 25 22 / / SYM cord-261579-f0prnpsu 25 23 T T NNP cord-261579-f0prnpsu 25 24 , , , cord-261579-f0prnpsu 25 25 in in IN cord-261579-f0prnpsu 25 26 which which WDT cord-261579-f0prnpsu 25 27 X x NN cord-261579-f0prnpsu 25 28 can can MD cord-261579-f0prnpsu 25 29 be be VB cord-261579-f0prnpsu 25 30 any any DT cord-261579-f0prnpsu 25 31 amino amino NN cord-261579-f0prnpsu 25 32 acid acid NN cord-261579-f0prnpsu 25 33 except except IN cord-261579-f0prnpsu 25 34 proline proline NN cord-261579-f0prnpsu 25 35 ) ) -RRB- cord-261579-f0prnpsu 25 36 , , , cord-261579-f0prnpsu 25 37 where where WRB cord-261579-f0prnpsu 25 38 seven seven CD cord-261579-f0prnpsu 25 39 of of IN cord-261579-f0prnpsu 25 40 these these DT cord-261579-f0prnpsu 25 41 are be VBP cord-261579-f0prnpsu 25 42 located locate VBN cord-261579-f0prnpsu 25 43 in in IN cord-261579-f0prnpsu 25 44 the the DT cord-261579-f0prnpsu 25 45 GP1-and GP1-and NNP cord-261579-f0prnpsu 25 46 four four CD cord-261579-f0prnpsu 25 47 in in IN cord-261579-f0prnpsu 25 48 the the DT cord-261579-f0prnpsu 25 49 GP2subunit GP2subunit NNP cord-261579-f0prnpsu 25 50 ( ( -LRB- cord-261579-f0prnpsu 25 51 Table table NN cord-261579-f0prnpsu 25 52 1 1 CD cord-261579-f0prnpsu 25 53 ) ) -RRB- cord-261579-f0prnpsu 25 54 . . . cord-261579-f0prnpsu 26 1 Using use VBG cord-261579-f0prnpsu 26 2 limited limited JJ cord-261579-f0prnpsu 26 3 N N NNP cord-261579-f0prnpsu 26 4 - - HYPH cord-261579-f0prnpsu 26 5 Glycanase Glycanase NNP cord-261579-f0prnpsu 26 6 digestion digestion NN cord-261579-f0prnpsu 26 7 , , , cord-261579-f0prnpsu 26 8 Wright Wright NNP cord-261579-f0prnpsu 26 9 et et NNP cord-261579-f0prnpsu 26 10 al al NNP cord-261579-f0prnpsu 26 11 . . . cord-261579-f0prnpsu 27 1 [ [ -LRB- cord-261579-f0prnpsu 27 2 22 22 CD cord-261579-f0prnpsu 27 3 ] ] -RRB- cord-261579-f0prnpsu 27 4 demonstrated demonstrate VBN cord-261579-f0prnpsu 27 5 for for IN cord-261579-f0prnpsu 27 6 LCMV LCMV NNP cord-261579-f0prnpsu 27 7 GP GP NNP cord-261579-f0prnpsu 27 8 that that IN cord-261579-f0prnpsu 27 9 five five CD cord-261579-f0prnpsu 27 10 potential potential JJ cord-261579-f0prnpsu 27 11 glycosylation glycosylation NN cord-261579-f0prnpsu 27 12 sites site NNS cord-261579-f0prnpsu 27 13 on on IN cord-261579-f0prnpsu 27 14 GP-1 GP-1 NNP cord-261579-f0prnpsu 27 15 were be VBD cord-261579-f0prnpsu 27 16 utilized utilize VBN cord-261579-f0prnpsu 27 17 and and CC cord-261579-f0prnpsu 27 18 two two CD cord-261579-f0prnpsu 27 19 of of IN cord-261579-f0prnpsu 27 20 the the DT cord-261579-f0prnpsu 27 21 three three CD cord-261579-f0prnpsu 27 22 sites site NNS cord-261579-f0prnpsu 27 23 on on IN cord-261579-f0prnpsu 27 24 GP-2 GP-2 NNP cord-261579-f0prnpsu 27 25 . . . cord-261579-f0prnpsu 28 1 However however RB cord-261579-f0prnpsu 28 2 , , , cord-261579-f0prnpsu 28 3 for for IN cord-261579-f0prnpsu 28 4 Lassa Lassa NNP cord-261579-f0prnpsu 28 5 virus virus NN cord-261579-f0prnpsu 28 6 GP gp NN cord-261579-f0prnpsu 28 7 it -PRON- PRP cord-261579-f0prnpsu 28 8 has have VBZ cord-261579-f0prnpsu 28 9 not not RB cord-261579-f0prnpsu 28 10 yet yet RB cord-261579-f0prnpsu 28 11 been be VBN cord-261579-f0prnpsu 28 12 shown show VBN cord-261579-f0prnpsu 28 13 which which WDT cord-261579-f0prnpsu 28 14 sites site NNS cord-261579-f0prnpsu 28 15 are be VBP cord-261579-f0prnpsu 28 16 actually actually RB cord-261579-f0prnpsu 28 17 used use VBN cord-261579-f0prnpsu 28 18 and and CC cord-261579-f0prnpsu 28 19 how how WRB cord-261579-f0prnpsu 28 20 N n NN cord-261579-f0prnpsu 28 21 - - HYPH cord-261579-f0prnpsu 28 22 glycosylation glycosylation NN cord-261579-f0prnpsu 28 23 affects affect VBZ cord-261579-f0prnpsu 28 24 the the DT cord-261579-f0prnpsu 28 25 characteristics characteristic NNS cord-261579-f0prnpsu 28 26 of of IN cord-261579-f0prnpsu 28 27 the the DT cord-261579-f0prnpsu 28 28 glycoprotein glycoprotein NN cord-261579-f0prnpsu 28 29 in in IN cord-261579-f0prnpsu 28 30 respect respect NN cord-261579-f0prnpsu 28 31 to to IN cord-261579-f0prnpsu 28 32 cleavage cleavage NN cord-261579-f0prnpsu 28 33 and and CC cord-261579-f0prnpsu 28 34 transport transport NN cord-261579-f0prnpsu 28 35 along along IN cord-261579-f0prnpsu 28 36 the the DT cord-261579-f0prnpsu 28 37 exocytotic exocytotic JJ cord-261579-f0prnpsu 28 38 pathway pathway NN cord-261579-f0prnpsu 28 39 . . . cord-261579-f0prnpsu 29 1 To to TO cord-261579-f0prnpsu 29 2 address address VB cord-261579-f0prnpsu 29 3 this this DT cord-261579-f0prnpsu 29 4 question question NN cord-261579-f0prnpsu 29 5 , , , cord-261579-f0prnpsu 29 6 we -PRON- PRP cord-261579-f0prnpsu 29 7 investigated investigate VBD cord-261579-f0prnpsu 29 8 in in IN cord-261579-f0prnpsu 29 9 the the DT cord-261579-f0prnpsu 29 10 present present JJ cord-261579-f0prnpsu 29 11 report report NN cord-261579-f0prnpsu 29 12 each each DT cord-261579-f0prnpsu 29 13 potential potential JJ cord-261579-f0prnpsu 29 14 N n CD cord-261579-f0prnpsu 29 15 - - HYPH cord-261579-f0prnpsu 29 16 glycosylation glycosylation NN cord-261579-f0prnpsu 29 17 site site NN cord-261579-f0prnpsu 29 18 of of IN cord-261579-f0prnpsu 29 19 the the DT cord-261579-f0prnpsu 29 20 Lassa Lassa NNP cord-261579-f0prnpsu 29 21 virus virus NN cord-261579-f0prnpsu 29 22 glycoprotein glycoprotein NN cord-261579-f0prnpsu 29 23 concerning concern VBG cord-261579-f0prnpsu 29 24 N n CD cord-261579-f0prnpsu 29 25 - - HYPH cord-261579-f0prnpsu 29 26 glycan glycan NN cord-261579-f0prnpsu 29 27 maturation maturation NN cord-261579-f0prnpsu 29 28 , , , cord-261579-f0prnpsu 29 29 cleavage cleavage NN cord-261579-f0prnpsu 29 30 of of IN cord-261579-f0prnpsu 29 31 GP gp NN cord-261579-f0prnpsu 29 32 - - HYPH cord-261579-f0prnpsu 29 33 C c NN cord-261579-f0prnpsu 29 34 and and CC cord-261579-f0prnpsu 29 35 glycoprotein glycoprotein NN cord-261579-f0prnpsu 29 36 transport transport NN cord-261579-f0prnpsu 29 37 to to IN cord-261579-f0prnpsu 29 38 the the DT cord-261579-f0prnpsu 29 39 cell cell NN cord-261579-f0prnpsu 29 40 surface surface NN cord-261579-f0prnpsu 29 41 . . . cord-261579-f0prnpsu 30 1 We -PRON- PRP cord-261579-f0prnpsu 30 2 provide provide VBP cord-261579-f0prnpsu 30 3 evidence evidence NN cord-261579-f0prnpsu 30 4 that that IN cord-261579-f0prnpsu 30 5 each each DT cord-261579-f0prnpsu 30 6 of of IN cord-261579-f0prnpsu 30 7 the the DT cord-261579-f0prnpsu 30 8 11 11 CD cord-261579-f0prnpsu 30 9 N n CD cord-261579-f0prnpsu 30 10 - - HYPH cord-261579-f0prnpsu 30 11 glycosylation glycosylation NN cord-261579-f0prnpsu 30 12 sites site NNS cord-261579-f0prnpsu 30 13 is be VBZ cord-261579-f0prnpsu 30 14 used use VBN cord-261579-f0prnpsu 30 15 for for IN cord-261579-f0prnpsu 30 16 N n CD cord-261579-f0prnpsu 30 17 - - HYPH cord-261579-f0prnpsu 30 18 glycan glycan NN cord-261579-f0prnpsu 30 19 attachment attachment NN cord-261579-f0prnpsu 30 20 . . . cord-261579-f0prnpsu 31 1 However however RB cord-261579-f0prnpsu 31 2 , , , cord-261579-f0prnpsu 31 3 seven seven CD cord-261579-f0prnpsu 31 4 N n CD cord-261579-f0prnpsu 31 5 - - HYPH cord-261579-f0prnpsu 31 6 glycan glycan NN cord-261579-f0prnpsu 31 7 mutants mutant NNS cord-261579-f0prnpsu 31 8 failed fail VBD cord-261579-f0prnpsu 31 9 in in IN cord-261579-f0prnpsu 31 10 their -PRON- PRP$ cord-261579-f0prnpsu 31 11 ability ability NN cord-261579-f0prnpsu 31 12 to to TO cord-261579-f0prnpsu 31 13 get get VB cord-261579-f0prnpsu 31 14 proteolytically proteolytically RB cord-261579-f0prnpsu 31 15 processed process VBN cord-261579-f0prnpsu 31 16 , , , cord-261579-f0prnpsu 31 17 while while IN cord-261579-f0prnpsu 31 18 4 4 CD cord-261579-f0prnpsu 31 19 N n CD cord-261579-f0prnpsu 31 20 - - HYPH cord-261579-f0prnpsu 31 21 glycosylation glycosylation NN cord-261579-f0prnpsu 31 22 sites site NNS cord-261579-f0prnpsu 31 23 are be VBP cord-261579-f0prnpsu 31 24 individually individually RB cord-261579-f0prnpsu 31 25 dispensable dispensable JJ cord-261579-f0prnpsu 31 26 for for IN cord-261579-f0prnpsu 31 27 cleavage cleavage NN cord-261579-f0prnpsu 31 28 of of IN cord-261579-f0prnpsu 31 29 GP gp NN cord-261579-f0prnpsu 31 30 - - HYPH cord-261579-f0prnpsu 31 31 C. c. NN cord-261579-f0prnpsu 32 1 Furthermore furthermore RB cord-261579-f0prnpsu 32 2 , , , cord-261579-f0prnpsu 32 3 we -PRON- PRP cord-261579-f0prnpsu 32 4 demonstrate demonstrate VBP cord-261579-f0prnpsu 32 5 that that IN cord-261579-f0prnpsu 32 6 both both DT cord-261579-f0prnpsu 32 7 uncleaved uncleaved JJ cord-261579-f0prnpsu 32 8 precursor precursor NN cord-261579-f0prnpsu 32 9 and and CC cord-261579-f0prnpsu 32 10 cleavage cleavage NN cord-261579-f0prnpsu 32 11 - - HYPH cord-261579-f0prnpsu 32 12 defective defective JJ cord-261579-f0prnpsu 32 13 N n CD cord-261579-f0prnpsu 32 14 - - HYPH cord-261579-f0prnpsu 32 15 glycan glycan NN cord-261579-f0prnpsu 32 16 mutants mutant NNS cord-261579-f0prnpsu 32 17 are be VBP cord-261579-f0prnpsu 32 18 transported transport VBN cord-261579-f0prnpsu 32 19 to to IN cord-261579-f0prnpsu 32 20 the the DT cord-261579-f0prnpsu 32 21 cell cell NN cord-261579-f0prnpsu 32 22 surface surface NN cord-261579-f0prnpsu 32 23 and and CC cord-261579-f0prnpsu 32 24 remain remain VB cord-261579-f0prnpsu 32 25 mannose mannose NN cord-261579-f0prnpsu 32 26 - - HYPH cord-261579-f0prnpsu 32 27 rich rich JJ cord-261579-f0prnpsu 32 28 . . . cord-261579-f0prnpsu 33 1 Vero Vero NNP cord-261579-f0prnpsu 33 2 cells cell NNS cord-261579-f0prnpsu 33 3 were be VBD cord-261579-f0prnpsu 33 4 cultured cultured JJ cord-261579-f0prnpsu 33 5 in in IN cord-261579-f0prnpsu 33 6 Dulbecco Dulbecco NNP cord-261579-f0prnpsu 33 7 's 's POS cord-261579-f0prnpsu 33 8 Modified modify VBN cord-261579-f0prnpsu 33 9 Eagle Eagle NNP cord-261579-f0prnpsu 33 10 's 's POS cord-261579-f0prnpsu 33 11 Medium Medium NNP cord-261579-f0prnpsu 33 12 ( ( -LRB- cord-261579-f0prnpsu 33 13 DMEM DMEM NNP cord-261579-f0prnpsu 33 14 , , , cord-261579-f0prnpsu 33 15 GIBCO GIBCO NNP cord-261579-f0prnpsu 33 16 ) ) -RRB- cord-261579-f0prnpsu 33 17 supplemented supplement VBD cord-261579-f0prnpsu 33 18 with with IN cord-261579-f0prnpsu 33 19 10 10 CD cord-261579-f0prnpsu 33 20 % % NN cord-261579-f0prnpsu 33 21 fetal fetal JJ cord-261579-f0prnpsu 33 22 calf calf NN cord-261579-f0prnpsu 33 23 serum serum NN cord-261579-f0prnpsu 33 24 ( ( -LRB- cord-261579-f0prnpsu 33 25 FCS FCS NNP cord-261579-f0prnpsu 33 26 ) ) -RRB- cord-261579-f0prnpsu 33 27 , , , cord-261579-f0prnpsu 33 28 100 100 CD cord-261579-f0prnpsu 33 29 units unit NNS cord-261579-f0prnpsu 33 30 / / SYM cord-261579-f0prnpsu 33 31 ml ml NN cord-261579-f0prnpsu 33 32 penicillin penicillin NN cord-261579-f0prnpsu 33 33 , , , cord-261579-f0prnpsu 33 34 and and CC cord-261579-f0prnpsu 33 35 0.1 0.1 CD cord-261579-f0prnpsu 33 36 mg mg NNP cord-261579-f0prnpsu 33 37 / / SYM cord-261579-f0prnpsu 33 38 ml ml NN cord-261579-f0prnpsu 33 39 streptomycin streptomycin NNS cord-261579-f0prnpsu 33 40 . . . cord-261579-f0prnpsu 34 1 Oligopeptide oligopeptide NN cord-261579-f0prnpsu 34 2 comprising comprise VBG cord-261579-f0prnpsu 34 3 the the DT cord-261579-f0prnpsu 34 4 amino amino NN cord-261579-f0prnpsu 34 5 acids acid NNS cord-261579-f0prnpsu 34 6 477 477 CD cord-261579-f0prnpsu 34 7 - - SYM cord-261579-f0prnpsu 34 8 491 491 CD cord-261579-f0prnpsu 34 9 of of IN cord-261579-f0prnpsu 34 10 preGP preGP NNP cord-261579-f0prnpsu 34 11 - - HYPH cord-261579-f0prnpsu 34 12 C C NNP cord-261579-f0prnpsu 34 13 was be VBD cord-261579-f0prnpsu 34 14 chemically chemically RB cord-261579-f0prnpsu 34 15 synthesized synthesize VBN cord-261579-f0prnpsu 34 16 and and CC cord-261579-f0prnpsu 34 17 covalently covalently RB cord-261579-f0prnpsu 34 18 linked link VBN cord-261579-f0prnpsu 34 19 to to IN cord-261579-f0prnpsu 34 20 keyhole keyhole VB cord-261579-f0prnpsu 34 21 limpet limpet NNP cord-261579-f0prnpsu 34 22 hemocyanin hemocyanin NNP cord-261579-f0prnpsu 34 23 ( ( -LRB- cord-261579-f0prnpsu 34 24 KLH KLH NNP cord-261579-f0prnpsu 34 25 , , , cord-261579-f0prnpsu 34 26 Pierce Pierce NNP cord-261579-f0prnpsu 34 27 ) ) -RRB- cord-261579-f0prnpsu 34 28 as as IN cord-261579-f0prnpsu 34 29 a a DT cord-261579-f0prnpsu 34 30 carrier carrier NN cord-261579-f0prnpsu 34 31 protein protein NN cord-261579-f0prnpsu 34 32 by by IN cord-261579-f0prnpsu 34 33 the the DT cord-261579-f0prnpsu 34 34 cross cross JJ cord-261579-f0prnpsu 34 35 - - JJ cord-261579-f0prnpsu 34 36 linker linker JJ cord-261579-f0prnpsu 34 37 agent agent NN cord-261579-f0prnpsu 34 38 N-[α n-[α NN cord-261579-f0prnpsu 34 39 - - HYPH cord-261579-f0prnpsu 34 40 maleimidoacetoxy maleimidoacetoxy NNP cord-261579-f0prnpsu 34 41 ] ] -RRB- cord-261579-f0prnpsu 34 42 succinimide succinimide NN cord-261579-f0prnpsu 34 43 ester ester NN cord-261579-f0prnpsu 34 44 ( ( -LRB- cord-261579-f0prnpsu 34 45 AMAS AMAS NNP cord-261579-f0prnpsu 34 46 ) ) -RRB- cord-261579-f0prnpsu 34 47 and and CC cord-261579-f0prnpsu 34 48 used use VBN cord-261579-f0prnpsu 34 49 for for IN cord-261579-f0prnpsu 34 50 repeated repeat VBN cord-261579-f0prnpsu 34 51 immunization immunization NN cord-261579-f0prnpsu 34 52 of of IN cord-261579-f0prnpsu 34 53 rabbits rabbit NNS cord-261579-f0prnpsu 34 54 as as IN cord-261579-f0prnpsu 34 55 described describe VBN cord-261579-f0prnpsu 34 56 before before IN cord-261579-f0prnpsu 34 57 [ [ -LRB- cord-261579-f0prnpsu 34 58 7 7 CD cord-261579-f0prnpsu 34 59 ] ] -RRB- cord-261579-f0prnpsu 34 60 . . . cord-261579-f0prnpsu 35 1 The the DT cord-261579-f0prnpsu 35 2 obtained obtain VBN cord-261579-f0prnpsu 35 3 antiserum antiserum NN cord-261579-f0prnpsu 35 4 Rb rb NN cord-261579-f0prnpsu 35 5 - - HYPH cord-261579-f0prnpsu 35 6 α α NNP cord-261579-f0prnpsu 35 7 - - HYPH cord-261579-f0prnpsu 35 8 GP2 GP2 NNP cord-261579-f0prnpsu 35 9 was be VBD cord-261579-f0prnpsu 35 10 tested test VBN cord-261579-f0prnpsu 35 11 by by IN cord-261579-f0prnpsu 35 12 peptide peptide JJ cord-261579-f0prnpsu 35 13 standard standard NN cord-261579-f0prnpsu 35 14 ELISA ELISA NNP cord-261579-f0prnpsu 35 15 [ [ -LRB- cord-261579-f0prnpsu 35 16 18 18 CD cord-261579-f0prnpsu 35 17 ] ] -RRB- cord-261579-f0prnpsu 35 18 and and CC cord-261579-f0prnpsu 35 19 used use VBN cord-261579-f0prnpsu 35 20 for for IN cord-261579-f0prnpsu 35 21 immunoblot immunoblot NNP cord-261579-f0prnpsu 35 22 analyses analysis NNS cord-261579-f0prnpsu 35 23 . . . cord-261579-f0prnpsu 36 1 The the DT cord-261579-f0prnpsu 36 2 full full JJ cord-261579-f0prnpsu 36 3 length length NN cord-261579-f0prnpsu 36 4 glycoprotein glycoprotein NN cord-261579-f0prnpsu 36 5 of of IN cord-261579-f0prnpsu 36 6 Lassa Lassa NNP cord-261579-f0prnpsu 36 7 virus virus NN cord-261579-f0prnpsu 36 8 ( ( -LRB- cord-261579-f0prnpsu 36 9 strain strain VBP cord-261579-f0prnpsu 36 10 Josiah Josiah NNP cord-261579-f0prnpsu 36 11 ) ) -RRB- cord-261579-f0prnpsu 36 12 was be VBD cord-261579-f0prnpsu 36 13 expressed express VBN cord-261579-f0prnpsu 36 14 using use VBG cord-261579-f0prnpsu 36 15 the the DT cord-261579-f0prnpsu 36 16 beta beta NN cord-261579-f0prnpsu 36 17 - - HYPH cord-261579-f0prnpsu 36 18 actin actin JJ cord-261579-f0prnpsu 36 19 promotor promotor NN cord-261579-f0prnpsu 36 20 - - HYPH cord-261579-f0prnpsu 36 21 driven drive VBN cord-261579-f0prnpsu 36 22 pCAGGS pcaggs NN cord-261579-f0prnpsu 36 23 vector vector NN cord-261579-f0prnpsu 36 24 [ [ -LRB- cord-261579-f0prnpsu 36 25 6 6 CD cord-261579-f0prnpsu 36 26 , , , cord-261579-f0prnpsu 36 27 23 23 CD cord-261579-f0prnpsu 36 28 ] ] -RRB- cord-261579-f0prnpsu 36 29 . . . cord-261579-f0prnpsu 37 1 Lassa Lassa NNP cord-261579-f0prnpsu 37 2 virus virus NN cord-261579-f0prnpsu 37 3 N n CD cord-261579-f0prnpsu 37 4 - - HYPH cord-261579-f0prnpsu 37 5 glycosylation glycosylation NN cord-261579-f0prnpsu 37 6 mutants mutant NNS cord-261579-f0prnpsu 37 7 were be VBD cord-261579-f0prnpsu 37 8 generated generate VBN cord-261579-f0prnpsu 37 9 by by IN cord-261579-f0prnpsu 37 10 recombinant recombinant JJ cord-261579-f0prnpsu 37 11 PCR PCR NNP cord-261579-f0prnpsu 37 12 using use VBG cord-261579-f0prnpsu 37 13 overlapping overlap VBG cord-261579-f0prnpsu 37 14 oligonucleotides oligonucleotide NNS cord-261579-f0prnpsu 37 15 , , , cord-261579-f0prnpsu 37 16 which which WDT cord-261579-f0prnpsu 37 17 will will MD cord-261579-f0prnpsu 37 18 be be VB cord-261579-f0prnpsu 37 19 made make VBN cord-261579-f0prnpsu 37 20 available available JJ cord-261579-f0prnpsu 37 21 on on IN cord-261579-f0prnpsu 37 22 request request NN cord-261579-f0prnpsu 37 23 . . . cord-261579-f0prnpsu 38 1 Sequences sequence NNS cord-261579-f0prnpsu 38 2 of of IN cord-261579-f0prnpsu 38 3 mutants mutant NNS cord-261579-f0prnpsu 38 4 were be VBD cord-261579-f0prnpsu 38 5 confirmed confirm VBN cord-261579-f0prnpsu 38 6 by by IN cord-261579-f0prnpsu 38 7 DNA dna NN cord-261579-f0prnpsu 38 8 sequencing sequencing NN cord-261579-f0prnpsu 38 9 . . . cord-261579-f0prnpsu 39 1 Vero Vero NNP cord-261579-f0prnpsu 39 2 cells cell NNS cord-261579-f0prnpsu 39 3 were be VBD cord-261579-f0prnpsu 39 4 transfected transfecte VBN cord-261579-f0prnpsu 39 5 with with IN cord-261579-f0prnpsu 39 6 wild wild JJ cord-261579-f0prnpsu 39 7 type type NN cord-261579-f0prnpsu 39 8 and and CC cord-261579-f0prnpsu 39 9 mutated mutate VBN cord-261579-f0prnpsu 39 10 recombinant recombinant JJ cord-261579-f0prnpsu 39 11 DNA dna NN cord-261579-f0prnpsu 39 12 using use VBG cord-261579-f0prnpsu 39 13 Lipofectamine Lipofectamine NNP cord-261579-f0prnpsu 39 14 2000 2000 CD cord-261579-f0prnpsu 40 1 ( ( -LRB- cord-261579-f0prnpsu 40 2 Gibco Gibco NNP cord-261579-f0prnpsu 40 3 / / SYM cord-261579-f0prnpsu 40 4 Invitrogen Invitrogen NNP cord-261579-f0prnpsu 40 5 ) ) -RRB- cord-261579-f0prnpsu 40 6 . . . cord-261579-f0prnpsu 40 7 _SP cord-261579-f0prnpsu 41 1 Lassa Lassa NNP cord-261579-f0prnpsu 41 2 virus virus NN cord-261579-f0prnpsu 41 3 preGP preGP NNP cord-261579-f0prnpsu 41 4 - - HYPH cord-261579-f0prnpsu 41 5 C C NNP cord-261579-f0prnpsu 41 6 contains contain VBZ cord-261579-f0prnpsu 41 7 11 11 CD cord-261579-f0prnpsu 41 8 potential potential JJ cord-261579-f0prnpsu 41 9 N n CD cord-261579-f0prnpsu 41 10 - - HYPH cord-261579-f0prnpsu 41 11 glycosylation glycosylation NN cord-261579-f0prnpsu 41 12 sites site NNS cord-261579-f0prnpsu 41 13 , , , cord-261579-f0prnpsu 41 14 7 7 CD cord-261579-f0prnpsu 41 15 in in IN cord-261579-f0prnpsu 41 16 the the DT cord-261579-f0prnpsu 41 17 GP-1-and GP-1-and NNP cord-261579-f0prnpsu 41 18 4 4 CD cord-261579-f0prnpsu 41 19 in in IN cord-261579-f0prnpsu 41 20 the the DT cord-261579-f0prnpsu 41 21 GP-2-subunit gp-2-subunit NN cord-261579-f0prnpsu 42 1 ( ( -LRB- cord-261579-f0prnpsu 42 2 Fig Fig NNP cord-261579-f0prnpsu 42 3 . . NNP cord-261579-f0prnpsu 42 4 1 1 CD cord-261579-f0prnpsu 42 5 ) ) -RRB- cord-261579-f0prnpsu 42 6 . . . cord-261579-f0prnpsu 43 1 To to TO cord-261579-f0prnpsu 43 2 investigate investigate VB cord-261579-f0prnpsu 43 3 whether whether IN cord-261579-f0prnpsu 43 4 each each DT cord-261579-f0prnpsu 43 5 N n CD cord-261579-f0prnpsu 43 6 - - HYPH cord-261579-f0prnpsu 43 7 glycosylation glycosylation NN cord-261579-f0prnpsu 43 8 site site NN cord-261579-f0prnpsu 43 9 is be VBZ cord-261579-f0prnpsu 43 10 used use VBN cord-261579-f0prnpsu 43 11 for for IN cord-261579-f0prnpsu 43 12 N n CD cord-261579-f0prnpsu 43 13 - - HYPH cord-261579-f0prnpsu 43 14 glycan glycan NN cord-261579-f0prnpsu 43 15 attachment attachment NN cord-261579-f0prnpsu 43 16 , , , cord-261579-f0prnpsu 43 17 the the DT cord-261579-f0prnpsu 43 18 corresponding corresponding JJ cord-261579-f0prnpsu 43 19 serine serine NN cord-261579-f0prnpsu 43 20 or or CC cord-261579-f0prnpsu 43 21 threonine threonine NN cord-261579-f0prnpsu 43 22 residues residue NNS cord-261579-f0prnpsu 43 23 of of IN cord-261579-f0prnpsu 43 24 potential potential JJ cord-261579-f0prnpsu 43 25 N n CD cord-261579-f0prnpsu 43 26 - - HYPH cord-261579-f0prnpsu 43 27 glycosylation glycosylation NN cord-261579-f0prnpsu 43 28 motifs motifs NN cord-261579-f0prnpsu 43 29 ( ( -LRB- cord-261579-f0prnpsu 43 30 N n CD cord-261579-f0prnpsu 43 31 - - HYPH cord-261579-f0prnpsu 43 32 X X NNP cord-261579-f0prnpsu 43 33 - - HYPH cord-261579-f0prnpsu 43 34 S/ S/ NNP cord-261579-f0prnpsu 43 35 T T NNP cord-261579-f0prnpsu 43 36 ) ) -RRB- cord-261579-f0prnpsu 43 37 were be VBD cord-261579-f0prnpsu 43 38 individually individually RB cord-261579-f0prnpsu 43 39 changed change VBN cord-261579-f0prnpsu 43 40 to to IN cord-261579-f0prnpsu 43 41 alanine alanine NN cord-261579-f0prnpsu 43 42 . . . cord-261579-f0prnpsu 44 1 GP-1 GP-1 NNP cord-261579-f0prnpsu 44 2 mutants mutant NNS cord-261579-f0prnpsu 44 3 were be VBD cord-261579-f0prnpsu 44 4 designated designate VBN cord-261579-f0prnpsu 44 5 T81A T81A NNP cord-261579-f0prnpsu 44 6 , , , cord-261579-f0prnpsu 44 7 S91A s91a JJ cord-261579-f0prnpsu 44 8 , , , cord-261579-f0prnpsu 44 9 T101A T101A NNP cord-261579-f0prnpsu 44 10 , , , cord-261579-f0prnpsu 44 11 S111A S111A NNP cord-261579-f0prnpsu 44 12 , , , cord-261579-f0prnpsu 44 13 S121A S121A NNP cord-261579-f0prnpsu 44 14 , , , cord-261579-f0prnpsu 44 15 S169A S169A NNP cord-261579-f0prnpsu 44 16 and and CC cord-261579-f0prnpsu 44 17 T226A T226A NNP cord-261579-f0prnpsu 44 18 and and CC cord-261579-f0prnpsu 44 19 GP-2 GP-2 NNP cord-261579-f0prnpsu 44 20 mutants mutant NNS cord-261579-f0prnpsu 44 21 S367A S367A NNP cord-261579-f0prnpsu 44 22 , , , cord-261579-f0prnpsu 44 23 S375A S375A NNP cord-261579-f0prnpsu 44 24 , , , cord-261579-f0prnpsu 44 25 S392A S392A NNP cord-261579-f0prnpsu 44 26 and and CC cord-261579-f0prnpsu 44 27 S397A. S397A. NNS cord-261579-f0prnpsu 45 1 All all DT cord-261579-f0prnpsu 45 2 cDNA cdna NN cord-261579-f0prnpsu 45 3 constructs construct NNS cord-261579-f0prnpsu 45 4 were be VBD cord-261579-f0prnpsu 45 5 expressed express VBN cord-261579-f0prnpsu 45 6 transiently transiently RB cord-261579-f0prnpsu 45 7 in in IN cord-261579-f0prnpsu 45 8 Vero Vero NNP cord-261579-f0prnpsu 45 9 cells cell NNS cord-261579-f0prnpsu 45 10 using use VBG cord-261579-f0prnpsu 45 11 the the DT cord-261579-f0prnpsu 45 12 beta beta NN cord-261579-f0prnpsu 45 13 - - HYPH cord-261579-f0prnpsu 45 14 actin actin JJ cord-261579-f0prnpsu 45 15 promoterdriven promoterdriven NN cord-261579-f0prnpsu 45 16 mammalian mammalian JJ cord-261579-f0prnpsu 45 17 expression expression NN cord-261579-f0prnpsu 45 18 vector vector NN cord-261579-f0prnpsu 45 19 pCAGGS pcaggs NN cord-261579-f0prnpsu 45 20 . . . cord-261579-f0prnpsu 46 1 Western western JJ cord-261579-f0prnpsu 46 2 blotting blotting NN cord-261579-f0prnpsu 46 3 was be VBD cord-261579-f0prnpsu 46 4 performed perform VBN cord-261579-f0prnpsu 46 5 using use VBG cord-261579-f0prnpsu 46 6 antiserum antiserum NN cord-261579-f0prnpsu 46 7 directed direct VBN cord-261579-f0prnpsu 46 8 against against IN cord-261579-f0prnpsu 46 9 the the DT cord-261579-f0prnpsu 46 10 C C NNP cord-261579-f0prnpsu 46 11 - - HYPH cord-261579-f0prnpsu 46 12 terminus terminus NN cord-261579-f0prnpsu 46 13 of of IN cord-261579-f0prnpsu 46 14 GP-2 GP-2 NNP cord-261579-f0prnpsu 46 15 . . . cord-261579-f0prnpsu 47 1 As as IN cord-261579-f0prnpsu 47 2 shown show VBN cord-261579-f0prnpsu 47 3 in in IN cord-261579-f0prnpsu 47 4 Fig Fig NNP cord-261579-f0prnpsu 47 5 . . . cord-261579-f0prnpsu 48 1 2A 2a LS cord-261579-f0prnpsu 49 1 ( ( -LRB- cord-261579-f0prnpsu 49 2 lanes lane NNS cord-261579-f0prnpsu 49 3 2 2 CD cord-261579-f0prnpsu 49 4 - - SYM cord-261579-f0prnpsu 49 5 8 8 CD cord-261579-f0prnpsu 49 6 , , , cord-261579-f0prnpsu 49 7 upper upper JJ cord-261579-f0prnpsu 49 8 panel panel NN cord-261579-f0prnpsu 49 9 ) ) -RRB- cord-261579-f0prnpsu 49 10 and and CC cord-261579-f0prnpsu 49 11 Fig Fig NNP cord-261579-f0prnpsu 49 12 . . . cord-261579-f0prnpsu 49 13 2B 2B NNP cord-261579-f0prnpsu 49 14 ( ( -LRB- cord-261579-f0prnpsu 49 15 lanes lane NNS cord-261579-f0prnpsu 49 16 2 2 CD cord-261579-f0prnpsu 49 17 - - SYM cord-261579-f0prnpsu 49 18 5 5 CD cord-261579-f0prnpsu 49 19 , , , cord-261579-f0prnpsu 49 20 upper upper JJ cord-261579-f0prnpsu 49 21 panel panel NN cord-261579-f0prnpsu 49 22 ) ) -RRB- cord-261579-f0prnpsu 49 23 , , , cord-261579-f0prnpsu 49 24 all all DT cord-261579-f0prnpsu 49 25 mutants mutant NNS cord-261579-f0prnpsu 49 26 showed show VBD cord-261579-f0prnpsu 49 27 faster fast JJR cord-261579-f0prnpsu 49 28 migration migration NN cord-261579-f0prnpsu 49 29 on on IN cord-261579-f0prnpsu 49 30 a a DT cord-261579-f0prnpsu 49 31 SDS sds NN cord-261579-f0prnpsu 49 32 gel gel NN cord-261579-f0prnpsu 49 33 compared compare VBN cord-261579-f0prnpsu 49 34 to to IN cord-261579-f0prnpsu 49 35 wild wild JJ cord-261579-f0prnpsu 49 36 - - HYPH cord-261579-f0prnpsu 49 37 type type NN cord-261579-f0prnpsu 49 38 GP gp NN cord-261579-f0prnpsu 49 39 - - HYPH cord-261579-f0prnpsu 49 40 C C NNP cord-261579-f0prnpsu 49 41 indicating indicate VBG cord-261579-f0prnpsu 49 42 that that IN cord-261579-f0prnpsu 49 43 each each DT cord-261579-f0prnpsu 49 44 N n CD cord-261579-f0prnpsu 49 45 - - HYPH cord-261579-f0prnpsu 49 46 glycosylation glycosylation NN cord-261579-f0prnpsu 49 47 site site NN cord-261579-f0prnpsu 49 48 is be VBZ cord-261579-f0prnpsu 49 49 used use VBN cord-261579-f0prnpsu 49 50 for for IN cord-261579-f0prnpsu 49 51 N n CD cord-261579-f0prnpsu 49 52 - - HYPH cord-261579-f0prnpsu 49 53 glycan glycan NN cord-261579-f0prnpsu 49 54 attachment attachment NN cord-261579-f0prnpsu 49 55 . . . cord-261579-f0prnpsu 50 1 Next next RB cord-261579-f0prnpsu 50 2 we -PRON- PRP cord-261579-f0prnpsu 50 3 determined determine VBD cord-261579-f0prnpsu 50 4 the the DT cord-261579-f0prnpsu 50 5 effect effect NN cord-261579-f0prnpsu 50 6 of of IN cord-261579-f0prnpsu 50 7 the the DT cord-261579-f0prnpsu 50 8 individual individual JJ cord-261579-f0prnpsu 50 9 N n CD cord-261579-f0prnpsu 50 10 - - HYPH cord-261579-f0prnpsu 50 11 glycans glycan NNS cord-261579-f0prnpsu 50 12 on on IN cord-261579-f0prnpsu 50 13 the the DT cord-261579-f0prnpsu 50 14 maturation maturation NN cord-261579-f0prnpsu 50 15 cleavage cleavage NN cord-261579-f0prnpsu 50 16 by by IN cord-261579-f0prnpsu 50 17 analysing analyse VBG cord-261579-f0prnpsu 50 18 the the DT cord-261579-f0prnpsu 50 19 presence presence NN cord-261579-f0prnpsu 50 20 of of IN cord-261579-f0prnpsu 50 21 the the DT cord-261579-f0prnpsu 50 22 cleaved cleave VBN cord-261579-f0prnpsu 50 23 subunit subunit NN cord-261579-f0prnpsu 50 24 GP-2 GP-2 NNP cord-261579-f0prnpsu 50 25 . . . cord-261579-f0prnpsu 51 1 Disruption disruption NN cord-261579-f0prnpsu 51 2 of of IN cord-261579-f0prnpsu 51 3 the the DT cord-261579-f0prnpsu 51 4 N n CD cord-261579-f0prnpsu 51 5 - - HYPH cord-261579-f0prnpsu 51 6 glycosylation glycosylation NN cord-261579-f0prnpsu 51 7 sites site NNS cord-261579-f0prnpsu 51 8 within within IN cord-261579-f0prnpsu 51 9 the the DT cord-261579-f0prnpsu 51 10 GP-1 GP-1 NNP cord-261579-f0prnpsu 51 11 mutants mutant NNS cord-261579-f0prnpsu 51 12 S111A S111A NNP cord-261579-f0prnpsu 51 13 , , , cord-261579-f0prnpsu 51 14 S169A S169A NNP cord-261579-f0prnpsu 51 15 and and CC cord-261579-f0prnpsu 51 16 T226A T226A NNP cord-261579-f0prnpsu 51 17 has have VBZ cord-261579-f0prnpsu 51 18 no no DT cord-261579-f0prnpsu 51 19 influence influence NN cord-261579-f0prnpsu 51 20 on on IN cord-261579-f0prnpsu 51 21 GP gp NN cord-261579-f0prnpsu 51 22 - - HYPH cord-261579-f0prnpsu 51 23 C c NN cord-261579-f0prnpsu 51 24 cleavage cleavage NN cord-261579-f0prnpsu 52 1 ( ( -LRB- cord-261579-f0prnpsu 52 2 Fig Fig NNP cord-261579-f0prnpsu 52 3 . . . cord-261579-f0prnpsu 52 4 2A 2a CD cord-261579-f0prnpsu 52 5 , , , cord-261579-f0prnpsu 52 6 lanes lane VBZ cord-261579-f0prnpsu 52 7 5 5 CD cord-261579-f0prnpsu 52 8 , , , cord-261579-f0prnpsu 52 9 7 7 CD cord-261579-f0prnpsu 52 10 and and CC cord-261579-f0prnpsu 52 11 8 8 CD cord-261579-f0prnpsu 52 12 , , , cord-261579-f0prnpsu 52 13 lower low JJR cord-261579-f0prnpsu 52 14 panel panel NN cord-261579-f0prnpsu 52 15 ) ) -RRB- cord-261579-f0prnpsu 52 16 . . . cord-261579-f0prnpsu 53 1 In in IN cord-261579-f0prnpsu 53 2 contrast contrast NN cord-261579-f0prnpsu 53 3 , , , cord-261579-f0prnpsu 53 4 the the DT cord-261579-f0prnpsu 53 5 lack lack NN cord-261579-f0prnpsu 53 6 of of IN cord-261579-f0prnpsu 53 7 N n CD cord-261579-f0prnpsu 53 8 - - HYPH cord-261579-f0prnpsu 53 9 glycan glycan NN cord-261579-f0prnpsu 53 10 attachment attachment NN cord-261579-f0prnpsu 53 11 of of IN cord-261579-f0prnpsu 53 12 the the DT cord-261579-f0prnpsu 53 13 GP-1 GP-1 NNP cord-261579-f0prnpsu 53 14 mutants mutant NNS cord-261579-f0prnpsu 53 15 T81A T81A NNP cord-261579-f0prnpsu 53 16 , , , cord-261579-f0prnpsu 53 17 S91A s91a JJ cord-261579-f0prnpsu 53 18 , , , cord-261579-f0prnpsu 53 19 S101A S101A NNP cord-261579-f0prnpsu 53 20 and and CC cord-261579-f0prnpsu 53 21 S121A S121A NNP cord-261579-f0prnpsu 53 22 abolished abolish VBD cord-261579-f0prnpsu 53 23 the the DT cord-261579-f0prnpsu 53 24 proteolytical proteolytical JJ cord-261579-f0prnpsu 53 25 processing processing NN cord-261579-f0prnpsu 53 26 by by IN cord-261579-f0prnpsu 53 27 SKI-1 SKI-1 NNP cord-261579-f0prnpsu 53 28 / / SYM cord-261579-f0prnpsu 53 29 S1P s1p NN cord-261579-f0prnpsu 53 30 ( ( -LRB- cord-261579-f0prnpsu 53 31 Fig Fig NNP cord-261579-f0prnpsu 53 32 mutants mutant NNS cord-261579-f0prnpsu 53 33 S392A S392A NNP cord-261579-f0prnpsu 53 34 and and CC cord-261579-f0prnpsu 53 35 S397A S397A NNP cord-261579-f0prnpsu 53 36 revealed reveal VBD cord-261579-f0prnpsu 53 37 significantly significantly RB cord-261579-f0prnpsu 53 38 faster fast RBR cord-261579-f0prnpsu 53 39 electrophoretic electrophoretic JJ cord-261579-f0prnpsu 53 40 mobility mobility NN cord-261579-f0prnpsu 53 41 than than IN cord-261579-f0prnpsu 53 42 wild wild JJ cord-261579-f0prnpsu 53 43 type type NN cord-261579-f0prnpsu 54 1 GP-2 GP-2 NNP cord-261579-f0prnpsu 55 1 ( ( -LRB- cord-261579-f0prnpsu 55 2 Fig Fig NNP cord-261579-f0prnpsu 55 3 . . . cord-261579-f0prnpsu 55 4 2B 2b CD cord-261579-f0prnpsu 55 5 ; ; . cord-261579-f0prnpsu 56 1 lanes lane NNS cord-261579-f0prnpsu 56 2 4 4 CD cord-261579-f0prnpsu 56 3 - - SYM cord-261579-f0prnpsu 56 4 5 5 CD cord-261579-f0prnpsu 56 5 , , , cord-261579-f0prnpsu 56 6 lower low JJR cord-261579-f0prnpsu 56 7 panel panel NN cord-261579-f0prnpsu 56 8 ) ) -RRB- cord-261579-f0prnpsu 56 9 showing show VBG cord-261579-f0prnpsu 56 10 that that IN cord-261579-f0prnpsu 56 11 the the DT cord-261579-f0prnpsu 56 12 respective respective JJ cord-261579-f0prnpsu 56 13 N n CD cord-261579-f0prnpsu 56 14 - - HYPH cord-261579-f0prnpsu 56 15 glycosylation glycosylation NN cord-261579-f0prnpsu 56 16 sites site NNS cord-261579-f0prnpsu 56 17 are be VBP cord-261579-f0prnpsu 56 18 used use VBN cord-261579-f0prnpsu 56 19 for for IN cord-261579-f0prnpsu 56 20 N n CD cord-261579-f0prnpsu 56 21 - - HYPH cord-261579-f0prnpsu 56 22 glycan glycan NN cord-261579-f0prnpsu 56 23 attachment attachment NN cord-261579-f0prnpsu 56 24 . . . cord-261579-f0prnpsu 57 1 N n NN cord-261579-f0prnpsu 57 2 - - HYPH cord-261579-f0prnpsu 57 3 glycosylation glycosylation NN cord-261579-f0prnpsu 57 4 has have VBZ cord-261579-f0prnpsu 57 5 long long RB cord-261579-f0prnpsu 57 6 been be VBN cord-261579-f0prnpsu 57 7 known know VBN cord-261579-f0prnpsu 57 8 to to TO cord-261579-f0prnpsu 57 9 be be VB cord-261579-f0prnpsu 57 10 essential essential JJ cord-261579-f0prnpsu 57 11 for for IN cord-261579-f0prnpsu 57 12 correct correct JJ cord-261579-f0prnpsu 57 13 folding folding NN cord-261579-f0prnpsu 57 14 and and CC cord-261579-f0prnpsu 57 15 intracellular intracellular JJ cord-261579-f0prnpsu 57 16 transport transport NN cord-261579-f0prnpsu 57 17 of of IN cord-261579-f0prnpsu 57 18 viral viral JJ cord-261579-f0prnpsu 57 19 glyco glyco NNS cord-261579-f0prnpsu 57 20 - - HYPH cord-261579-f0prnpsu 57 21 proteins protein NNS cord-261579-f0prnpsu 57 22 , , , cord-261579-f0prnpsu 57 23 as as IN cord-261579-f0prnpsu 57 24 shown show VBN cord-261579-f0prnpsu 57 25 for for IN cord-261579-f0prnpsu 57 26 instances instance NNS cord-261579-f0prnpsu 57 27 for for IN cord-261579-f0prnpsu 57 28 influenza influenza NN cord-261579-f0prnpsu 57 29 hemagglutinin hemagglutinin NNS cord-261579-f0prnpsu 57 30 [ [ -LRB- cord-261579-f0prnpsu 57 31 19 19 CD cord-261579-f0prnpsu 57 32 ] ] -RRB- cord-261579-f0prnpsu 57 33 . . . cord-261579-f0prnpsu 58 1 In in IN cord-261579-f0prnpsu 58 2 order order NN cord-261579-f0prnpsu 58 3 to to TO cord-261579-f0prnpsu 58 4 investigate investigate VB cord-261579-f0prnpsu 58 5 the the DT cord-261579-f0prnpsu 58 6 importance importance NN cord-261579-f0prnpsu 58 7 of of IN cord-261579-f0prnpsu 58 8 the the DT cord-261579-f0prnpsu 58 9 individual individual JJ cord-261579-f0prnpsu 58 10 N n CD cord-261579-f0prnpsu 58 11 - - HYPH cord-261579-f0prnpsu 58 12 glycosylation glycosylation NN cord-261579-f0prnpsu 58 13 sites site NNS cord-261579-f0prnpsu 58 14 of of IN cord-261579-f0prnpsu 58 15 Lassa Lassa NNP cord-261579-f0prnpsu 58 16 virus virus NN cord-261579-f0prnpsu 58 17 GP gp NN cord-261579-f0prnpsu 58 18 - - HYPH cord-261579-f0prnpsu 58 19 C C NNP cord-261579-f0prnpsu 58 20 for for IN cord-261579-f0prnpsu 58 21 intracellular intracellular JJ cord-261579-f0prnpsu 58 22 trafficking trafficking NN cord-261579-f0prnpsu 58 23 we -PRON- PRP cord-261579-f0prnpsu 58 24 analysed analyse VBD cord-261579-f0prnpsu 58 25 cell cell NN cord-261579-f0prnpsu 58 26 surface surface NN cord-261579-f0prnpsu 58 27 expression expression NN cord-261579-f0prnpsu 58 28 of of IN cord-261579-f0prnpsu 58 29 the the DT cord-261579-f0prnpsu 58 30 mutated mutate VBN cord-261579-f0prnpsu 58 31 glycoproteins glycoprotein NNS cord-261579-f0prnpsu 58 32 using use VBG cord-261579-f0prnpsu 58 33 a a DT cord-261579-f0prnpsu 58 34 biotinylation biotinylation NN cord-261579-f0prnpsu 58 35 approach approach NN cord-261579-f0prnpsu 58 36 . . . cord-261579-f0prnpsu 59 1 Transfected Transfected NNP cord-261579-f0prnpsu 59 2 Vero Vero NNP cord-261579-f0prnpsu 59 3 cells cell NNS cord-261579-f0prnpsu 59 4 were be VBD cord-261579-f0prnpsu 59 5 labelled label VBN cord-261579-f0prnpsu 59 6 with with IN cord-261579-f0prnpsu 59 7 nonmembrane nonmembrane NN cord-261579-f0prnpsu 59 8 - - HYPH cord-261579-f0prnpsu 59 9 permeating permeate VBG cord-261579-f0prnpsu 59 10 biotin biotin NN cord-261579-f0prnpsu 59 11 as as IN cord-261579-f0prnpsu 59 12 described describe VBN cord-261579-f0prnpsu 59 13 in in IN cord-261579-f0prnpsu 59 14 material material NN cord-261579-f0prnpsu 59 15 and and CC cord-261579-f0prnpsu 59 16 methods method NNS cord-261579-f0prnpsu 59 17 . . . cord-261579-f0prnpsu 60 1 After after IN cord-261579-f0prnpsu 60 2 biotinylation biotinylation NN cord-261579-f0prnpsu 60 3 , , , cord-261579-f0prnpsu 60 4 cells cell NNS cord-261579-f0prnpsu 60 5 were be VBD cord-261579-f0prnpsu 60 6 lysed lyse VBN cord-261579-f0prnpsu 60 7 and and CC cord-261579-f0prnpsu 60 8 GP gp NN cord-261579-f0prnpsu 60 9 - - HYPH cord-261579-f0prnpsu 60 10 Proteolytic Proteolytic NNP cord-261579-f0prnpsu 60 11 processing processing NN cord-261579-f0prnpsu 60 12 of of IN cord-261579-f0prnpsu 60 13 N n CD cord-261579-f0prnpsu 60 14 - - HYPH cord-261579-f0prnpsu 60 15 glycosylation glycosylation NN cord-261579-f0prnpsu 60 16 mutants mutant NNS cord-261579-f0prnpsu 60 17 Figure figure VBP cord-261579-f0prnpsu 60 18 2 2 CD cord-261579-f0prnpsu 60 19 Proteolytic Proteolytic NNP cord-261579-f0prnpsu 60 20 processing processing NN cord-261579-f0prnpsu 60 21 of of IN cord-261579-f0prnpsu 60 22 N n CD cord-261579-f0prnpsu 60 23 - - HYPH cord-261579-f0prnpsu 60 24 glycosylation glycosylation NN cord-261579-f0prnpsu 60 25 mutants mutant NNS cord-261579-f0prnpsu 60 26 . . . cord-261579-f0prnpsu 61 1 A a DT cord-261579-f0prnpsu 61 2 Vero Vero NNP cord-261579-f0prnpsu 61 3 cells cell NNS cord-261579-f0prnpsu 61 4 expressing express VBG cord-261579-f0prnpsu 61 5 either either CC cord-261579-f0prnpsu 61 6 wild wild JJ cord-261579-f0prnpsu 61 7 - - HYPH cord-261579-f0prnpsu 61 8 type type NN cord-261579-f0prnpsu 61 9 GP gp NN cord-261579-f0prnpsu 61 10 - - HYPH cord-261579-f0prnpsu 61 11 C C NNP cord-261579-f0prnpsu 61 12 or or CC cord-261579-f0prnpsu 61 13 GP-1 GP-1 NNP cord-261579-f0prnpsu 61 14 N N NNP cord-261579-f0prnpsu 61 15 - - HYPH cord-261579-f0prnpsu 61 16 glycosylation glycosylation NN cord-261579-f0prnpsu 61 17 mutants mutant NNS cord-261579-f0prnpsu 61 18 T81A T81A NNP cord-261579-f0prnpsu 61 19 , , , cord-261579-f0prnpsu 61 20 S91A S91A NNP cord-261579-f0prnpsu 61 21 , , , cord-261579-f0prnpsu 61 22 T101A T101A NNP cord-261579-f0prnpsu 61 23 , , , cord-261579-f0prnpsu 61 24 S111A S111A NNP cord-261579-f0prnpsu 61 25 , , , cord-261579-f0prnpsu 61 26 S121A S121A NNP cord-261579-f0prnpsu 61 27 , , , cord-261579-f0prnpsu 61 28 S169A S169A NNP cord-261579-f0prnpsu 61 29 and and CC cord-261579-f0prnpsu 61 30 T226A. T226A. NNP cord-261579-f0prnpsu 61 31 B B NNP cord-261579-f0prnpsu 61 32 Vero Vero NNP cord-261579-f0prnpsu 61 33 cells cell NNS cord-261579-f0prnpsu 61 34 expressing express VBG cord-261579-f0prnpsu 61 35 either either CC cord-261579-f0prnpsu 61 36 wild wild JJ cord-261579-f0prnpsu 61 37 - - HYPH cord-261579-f0prnpsu 61 38 type type NN cord-261579-f0prnpsu 61 39 GP gp NN cord-261579-f0prnpsu 61 40 - - HYPH cord-261579-f0prnpsu 61 41 C C NNP cord-261579-f0prnpsu 61 42 or or CC cord-261579-f0prnpsu 61 43 GP-2 GP-2 NNP cord-261579-f0prnpsu 61 44 N n CD cord-261579-f0prnpsu 61 45 - - HYPH cord-261579-f0prnpsu 61 46 glycosylation glycosylation NN cord-261579-f0prnpsu 61 47 mutants mutant NNS cord-261579-f0prnpsu 61 48 S367A s367a NN cord-261579-f0prnpsu 61 49 , , , cord-261579-f0prnpsu 61 50 S375A S375A NNP cord-261579-f0prnpsu 61 51 , , , cord-261579-f0prnpsu 61 52 S392A S392A NNP cord-261579-f0prnpsu 61 53 and and CC cord-261579-f0prnpsu 61 54 S397A. S397A. NNS cord-261579-f0prnpsu 62 1 The the DT cord-261579-f0prnpsu 62 2 expressed express VBN cord-261579-f0prnpsu 62 3 proteins protein NNS cord-261579-f0prnpsu 62 4 were be VBD cord-261579-f0prnpsu 62 5 separated separate VBN cord-261579-f0prnpsu 62 6 by by IN cord-261579-f0prnpsu 62 7 SDS SDS NNP cord-261579-f0prnpsu 62 8 - - HYPH cord-261579-f0prnpsu 62 9 PAGE PAGE NNP cord-261579-f0prnpsu 62 10 on on IN cord-261579-f0prnpsu 62 11 12 12 CD cord-261579-f0prnpsu 62 12 % % NN cord-261579-f0prnpsu 62 13 acrylamide acrylamide NN cord-261579-f0prnpsu 62 14 gels gel NNS cord-261579-f0prnpsu 62 15 and and CC cord-261579-f0prnpsu 62 16 immunoblotted immunoblotte VBN cord-261579-f0prnpsu 62 17 . . . cord-261579-f0prnpsu 63 1 Non non JJ cord-261579-f0prnpsu 63 2 - - JJ cord-261579-f0prnpsu 63 3 cleaved cleaved JJ cord-261579-f0prnpsu 63 4 GP gp NN cord-261579-f0prnpsu 63 5 - - HYPH cord-261579-f0prnpsu 63 6 C C NNP cord-261579-f0prnpsu 63 7 and and CC cord-261579-f0prnpsu 63 8 its -PRON- PRP$ cord-261579-f0prnpsu 63 9 cleaved cleaved JJ cord-261579-f0prnpsu 63 10 form form NN cord-261579-f0prnpsu 64 1 GP-2 GP-2 NNP cord-261579-f0prnpsu 64 2 are be VBP cord-261579-f0prnpsu 64 3 detected detect VBN cord-261579-f0prnpsu 64 4 by by IN cord-261579-f0prnpsu 64 5 immunostaining immunostaine VBG cord-261579-f0prnpsu 64 6 using use VBG cord-261579-f0prnpsu 64 7 the the DT cord-261579-f0prnpsu 64 8 antiserum antiserum NN cord-261579-f0prnpsu 65 1 rb rb NN cord-261579-f0prnpsu 65 2 - - HYPH cord-261579-f0prnpsu 65 3 α α NN cord-261579-f0prnpsu 65 4 - - HYPH cord-261579-f0prnpsu 65 5 GP-2 GP-2 NNP cord-261579-f0prnpsu 65 6 . . . cord-261579-f0prnpsu 65 7 C C NNP cord-261579-f0prnpsu 65 8 and and CC cord-261579-f0prnpsu 65 9 GP GP NNP cord-261579-f0prnpsu 65 10 - - HYPH cord-261579-f0prnpsu 65 11 C C NNP cord-261579-f0prnpsu 65 12 N n NN cord-261579-f0prnpsu 65 13 - - HYPH cord-261579-f0prnpsu 65 14 glycan glycan NN cord-261579-f0prnpsu 65 15 mutants mutant NNS cord-261579-f0prnpsu 65 16 were be VBD cord-261579-f0prnpsu 65 17 immunoprecipitated immunoprecipitate VBN cord-261579-f0prnpsu 65 18 using use VBG cord-261579-f0prnpsu 65 19 polyclonal polyclonal JJ cord-261579-f0prnpsu 65 20 antiserum antiserum NN cord-261579-f0prnpsu 65 21 directed direct VBN cord-261579-f0prnpsu 65 22 against against IN cord-261579-f0prnpsu 65 23 the the DT cord-261579-f0prnpsu 65 24 C C NNP cord-261579-f0prnpsu 65 25 - - HYPH cord-261579-f0prnpsu 65 26 terminus terminus NN cord-261579-f0prnpsu 65 27 of of IN cord-261579-f0prnpsu 65 28 GP GP NNP cord-261579-f0prnpsu 65 29 - - HYPH cord-261579-f0prnpsu 65 30 C. C. NNP cord-261579-f0prnpsu 66 1 The the DT cord-261579-f0prnpsu 66 2 precipitates precipitate NNS cord-261579-f0prnpsu 66 3 were be VBD cord-261579-f0prnpsu 66 4 separated separate VBN cord-261579-f0prnpsu 66 5 on on IN cord-261579-f0prnpsu 66 6 an an DT cord-261579-f0prnpsu 66 7 SDS sds NN cord-261579-f0prnpsu 66 8 gel gel NN cord-261579-f0prnpsu 66 9 and and CC cord-261579-f0prnpsu 66 10 transferred transfer VBN cord-261579-f0prnpsu 66 11 to to IN cord-261579-f0prnpsu 66 12 nitrocellulose nitrocellulose NN cord-261579-f0prnpsu 66 13 . . . cord-261579-f0prnpsu 67 1 Surface surface NN cord-261579-f0prnpsu 67 2 - - HYPH cord-261579-f0prnpsu 67 3 expressed express VBN cord-261579-f0prnpsu 67 4 glycoprotein glycoprotein NN cord-261579-f0prnpsu 67 5 protein protein NN cord-261579-f0prnpsu 67 6 was be VBD cord-261579-f0prnpsu 67 7 then then RB cord-261579-f0prnpsu 67 8 visualised visualise VBN cord-261579-f0prnpsu 67 9 with with IN cord-261579-f0prnpsu 67 10 a a DT cord-261579-f0prnpsu 67 11 streptavidin streptavidin NN cord-261579-f0prnpsu 67 12 - - HYPH cord-261579-f0prnpsu 67 13 peroxidase peroxidase NN cord-261579-f0prnpsu 67 14 complex complex NN cord-261579-f0prnpsu 67 15 . . . cord-261579-f0prnpsu 68 1 Fig Fig NNP cord-261579-f0prnpsu 68 2 . . . cord-261579-f0prnpsu 69 1 3A 3A NNP cord-261579-f0prnpsu 69 2 ( ( -LRB- cord-261579-f0prnpsu 69 3 lanes lane NNS cord-261579-f0prnpsu 69 4 2 2 CD cord-261579-f0prnpsu 69 5 - - SYM cord-261579-f0prnpsu 69 6 8 8 CD cord-261579-f0prnpsu 69 7 ) ) -RRB- cord-261579-f0prnpsu 69 8 and and CC cord-261579-f0prnpsu 69 9 B B NNP cord-261579-f0prnpsu 69 10 ( ( -LRB- cord-261579-f0prnpsu 69 11 lanes lane NNS cord-261579-f0prnpsu 69 12 2 2 CD cord-261579-f0prnpsu 69 13 - - SYM cord-261579-f0prnpsu 69 14 5 5 CD cord-261579-f0prnpsu 69 15 ) ) -RRB- cord-261579-f0prnpsu 69 16 demonstrate demonstrate VBP cord-261579-f0prnpsu 69 17 that that IN cord-261579-f0prnpsu 69 18 all all DT cord-261579-f0prnpsu 69 19 GP-1 GP-1 NNP cord-261579-f0prnpsu 69 20 and and CC cord-261579-f0prnpsu 69 21 GP-2 GP-2 NNP cord-261579-f0prnpsu 69 22 N n CD cord-261579-f0prnpsu 69 23 - - HYPH cord-261579-f0prnpsu 69 24 glycosylation glycosylation NN cord-261579-f0prnpsu 69 25 mutants mutant NNS cord-261579-f0prnpsu 69 26 are be VBP cord-261579-f0prnpsu 69 27 transported transport VBN cord-261579-f0prnpsu 69 28 to to IN cord-261579-f0prnpsu 69 29 the the DT cord-261579-f0prnpsu 69 30 cell cell NN cord-261579-f0prnpsu 69 31 surface surface NN cord-261579-f0prnpsu 69 32 in in IN cord-261579-f0prnpsu 69 33 a a DT cord-261579-f0prnpsu 69 34 similar similar JJ cord-261579-f0prnpsu 69 35 manner manner NN cord-261579-f0prnpsu 69 36 compared compare VBN cord-261579-f0prnpsu 69 37 to to IN cord-261579-f0prnpsu 69 38 wild wild JJ cord-261579-f0prnpsu 69 39 - - HYPH cord-261579-f0prnpsu 69 40 type type NN cord-261579-f0prnpsu 69 41 GP gp NN cord-261579-f0prnpsu 69 42 indicating indicate VBG cord-261579-f0prnpsu 69 43 that that IN cord-261579-f0prnpsu 69 44 a a DT cord-261579-f0prnpsu 69 45 loss loss NN cord-261579-f0prnpsu 69 46 of of IN cord-261579-f0prnpsu 69 47 a a DT cord-261579-f0prnpsu 69 48 single single JJ cord-261579-f0prnpsu 69 49 N n CD cord-261579-f0prnpsu 69 50 - - HYPH cord-261579-f0prnpsu 69 51 glycosylation glycosylation NN cord-261579-f0prnpsu 69 52 attachment attachment NN cord-261579-f0prnpsu 69 53 site site NN cord-261579-f0prnpsu 69 54 and and CC cord-261579-f0prnpsu 69 55 proteolytic proteolytic JJ cord-261579-f0prnpsu 69 56 cleavage cleavage NN cord-261579-f0prnpsu 69 57 are be VBP cord-261579-f0prnpsu 69 58 not not RB cord-261579-f0prnpsu 69 59 necessary necessary JJ cord-261579-f0prnpsu 69 60 for for IN cord-261579-f0prnpsu 69 61 cell cell NN cord-261579-f0prnpsu 69 62 surface surface NN cord-261579-f0prnpsu 69 63 transport transport NN cord-261579-f0prnpsu 69 64 of of IN cord-261579-f0prnpsu 69 65 GP gp NN cord-261579-f0prnpsu 69 66 . . . cord-261579-f0prnpsu 70 1 For for IN cord-261579-f0prnpsu 70 2 LCMV LCMV NNP cord-261579-f0prnpsu 70 3 it -PRON- PRP cord-261579-f0prnpsu 70 4 was be VBD cord-261579-f0prnpsu 70 5 shown show VBN cord-261579-f0prnpsu 70 6 that that IN cord-261579-f0prnpsu 70 7 maturation maturation NN cord-261579-f0prnpsu 70 8 cleavage cleavage NN cord-261579-f0prnpsu 70 9 deficient deficient JJ cord-261579-f0prnpsu 70 10 mutants mutant NNS cord-261579-f0prnpsu 70 11 are be VBP cord-261579-f0prnpsu 70 12 efficiently efficiently RB cord-261579-f0prnpsu 70 13 transported transport VBN cord-261579-f0prnpsu 70 14 to to IN cord-261579-f0prnpsu 70 15 the the DT cord-261579-f0prnpsu 70 16 cell cell NN cord-261579-f0prnpsu 70 17 surface surface NN cord-261579-f0prnpsu 70 18 [ [ -LRB- cord-261579-f0prnpsu 70 19 10 10 CD cord-261579-f0prnpsu 70 20 ] ] -RRB- cord-261579-f0prnpsu 70 21 . . . cord-261579-f0prnpsu 71 1 Both both DT cord-261579-f0prnpsu 71 2 cleavage cleavage NN cord-261579-f0prnpsu 71 3 and and CC cord-261579-f0prnpsu 71 4 cell cell NN cord-261579-f0prnpsu 71 5 surface surface NN cord-261579-f0prnpsu 71 6 expression expression NN cord-261579-f0prnpsu 71 7 of of IN cord-261579-f0prnpsu 71 8 LCMV LCMV NNP cord-261579-f0prnpsu 71 9 GP GP NNP cord-261579-f0prnpsu 71 10 - - HYPH cord-261579-f0prnpsu 71 11 C C NNP cord-261579-f0prnpsu 71 12 are be VBP cord-261579-f0prnpsu 71 13 impaired impair VBN cord-261579-f0prnpsu 71 14 in in IN cord-261579-f0prnpsu 71 15 parallel parallel NN cord-261579-f0prnpsu 71 16 either either CC cord-261579-f0prnpsu 71 17 when when WRB cord-261579-f0prnpsu 71 18 no no DT cord-261579-f0prnpsu 71 19 N n NN cord-261579-f0prnpsu 71 20 - - HYPH cord-261579-f0prnpsu 71 21 glycosylation glycosylation NN cord-261579-f0prnpsu 71 22 occurs occur VBZ cord-261579-f0prnpsu 71 23 or or CC cord-261579-f0prnpsu 71 24 a a DT cord-261579-f0prnpsu 71 25 proline proline NN cord-261579-f0prnpsu 71 26 residue residue NN cord-261579-f0prnpsu 71 27 is be VBZ cord-261579-f0prnpsu 71 28 present present JJ cord-261579-f0prnpsu 71 29 at at IN cord-261579-f0prnpsu 71 30 position position NN cord-261579-f0prnpsu 71 31 110 110 CD cord-261579-f0prnpsu 71 32 [ [ -LRB- cord-261579-f0prnpsu 71 33 22 22 CD cord-261579-f0prnpsu 71 34 , , , cord-261579-f0prnpsu 71 35 24 24 CD cord-261579-f0prnpsu 71 36 ] ] -RRB- cord-261579-f0prnpsu 71 37 . . . cord-261579-f0prnpsu 72 1 Since since IN cord-261579-f0prnpsu 72 2 many many JJ cord-261579-f0prnpsu 72 3 cleavage cleavage NN cord-261579-f0prnpsu 72 4 deficient deficient JJ cord-261579-f0prnpsu 72 5 mutants mutant NNS cord-261579-f0prnpsu 72 6 have have VBP cord-261579-f0prnpsu 72 7 been be VBN cord-261579-f0prnpsu 72 8 shown show VBN cord-261579-f0prnpsu 72 9 to to TO cord-261579-f0prnpsu 72 10 exhibit exhibit VB cord-261579-f0prnpsu 72 11 regular regular JJ cord-261579-f0prnpsu 72 12 cell cell NN cord-261579-f0prnpsu 72 13 surface surface NN cord-261579-f0prnpsu 72 14 transport transport NN cord-261579-f0prnpsu 72 15 , , , cord-261579-f0prnpsu 72 16 the the DT cord-261579-f0prnpsu 72 17 two two CD cord-261579-f0prnpsu 72 18 reported report VBN cord-261579-f0prnpsu 72 19 cases case NNS cord-261579-f0prnpsu 72 20 in in IN cord-261579-f0prnpsu 72 21 which which WDT cord-261579-f0prnpsu 72 22 cell cell NN cord-261579-f0prnpsu 72 23 surface surface NN cord-261579-f0prnpsu 72 24 expression expression NN cord-261579-f0prnpsu 72 25 was be VBD cord-261579-f0prnpsu 72 26 abolished abolish VBN cord-261579-f0prnpsu 72 27 might may MD cord-261579-f0prnpsu 72 28 be be VB cord-261579-f0prnpsu 72 29 due due JJ cord-261579-f0prnpsu 72 30 to to IN cord-261579-f0prnpsu 72 31 misfolding misfolde VBG cord-261579-f0prnpsu 72 32 in in IN cord-261579-f0prnpsu 72 33 the the DT cord-261579-f0prnpsu 72 34 endoplasmic endoplasmic NN cord-261579-f0prnpsu 72 35 reticulum reticulum NN cord-261579-f0prnpsu 72 36 with with IN cord-261579-f0prnpsu 72 37 subsequent subsequent JJ cord-261579-f0prnpsu 72 38 degradation degradation NN cord-261579-f0prnpsu 72 39 [ [ -LRB- cord-261579-f0prnpsu 72 40 22 22 CD cord-261579-f0prnpsu 72 41 , , , cord-261579-f0prnpsu 72 42 24 24 CD cord-261579-f0prnpsu 72 43 ] ] -RRB- cord-261579-f0prnpsu 72 44 . . . cord-261579-f0prnpsu 73 1 Cell cell NN cord-261579-f0prnpsu 73 2 surface surface NN cord-261579-f0prnpsu 73 3 transport transport NN cord-261579-f0prnpsu 73 4 of of IN cord-261579-f0prnpsu 73 5 GP gp NN cord-261579-f0prnpsu 73 6 - - HYPH cord-261579-f0prnpsu 73 7 C C NNP cord-261579-f0prnpsu 73 8 N n NN cord-261579-f0prnpsu 73 9 - - HYPH cord-261579-f0prnpsu 73 10 glycosylation glycosylation NN cord-261579-f0prnpsu 73 11 mutants mutant NNS cord-261579-f0prnpsu 73 12 Figure figure VBP cord-261579-f0prnpsu 73 13 3 3 CD cord-261579-f0prnpsu 73 14 Cell cell NN cord-261579-f0prnpsu 73 15 surface surface NN cord-261579-f0prnpsu 73 16 transport transport NN cord-261579-f0prnpsu 73 17 of of IN cord-261579-f0prnpsu 73 18 GP gp NN cord-261579-f0prnpsu 73 19 - - HYPH cord-261579-f0prnpsu 73 20 C C NNP cord-261579-f0prnpsu 73 21 N n NN cord-261579-f0prnpsu 73 22 - - HYPH cord-261579-f0prnpsu 73 23 glycosylation glycosylation NN cord-261579-f0prnpsu 73 24 mutants mutant NNS cord-261579-f0prnpsu 73 25 . . . cord-261579-f0prnpsu 74 1 Vero Vero NNP cord-261579-f0prnpsu 74 2 cells cell NNS cord-261579-f0prnpsu 74 3 were be VBD cord-261579-f0prnpsu 74 4 transfected transfecte VBN cord-261579-f0prnpsu 74 5 with with IN cord-261579-f0prnpsu 74 6 GP-1 GP-1 NNP cord-261579-f0prnpsu 74 7 ( ( -LRB- cord-261579-f0prnpsu 74 8 A a NN cord-261579-f0prnpsu 74 9 ) ) -RRB- cord-261579-f0prnpsu 74 10 and and CC cord-261579-f0prnpsu 74 11 GP-2 GP-2 NNP cord-261579-f0prnpsu 74 12 ( ( -LRB- cord-261579-f0prnpsu 74 13 B b NN cord-261579-f0prnpsu 74 14 ) ) -RRB- cord-261579-f0prnpsu 74 15 N n CD cord-261579-f0prnpsu 74 16 - - HYPH cord-261579-f0prnpsu 74 17 glycosylation glycosylation NN cord-261579-f0prnpsu 74 18 mutants mutant NNS cord-261579-f0prnpsu 74 19 of of IN cord-261579-f0prnpsu 74 20 Lassa Lassa NNP cord-261579-f0prnpsu 74 21 virus virus NN cord-261579-f0prnpsu 74 22 glycoprotein glycoprotein NN cord-261579-f0prnpsu 74 23 . . . cord-261579-f0prnpsu 75 1 At at IN cord-261579-f0prnpsu 75 2 24 24 CD cord-261579-f0prnpsu 75 3 h h NN cord-261579-f0prnpsu 75 4 post post NN cord-261579-f0prnpsu 75 5 - - NN cord-261579-f0prnpsu 75 6 transfection transfection JJ cord-261579-f0prnpsu 75 7 , , , cord-261579-f0prnpsu 75 8 cells cell NNS cord-261579-f0prnpsu 75 9 were be VBD cord-261579-f0prnpsu 75 10 surface surface NN cord-261579-f0prnpsu 75 11 labelled label VBN cord-261579-f0prnpsu 75 12 with with IN cord-261579-f0prnpsu 75 13 biotin biotin NN cord-261579-f0prnpsu 75 14 . . . cord-261579-f0prnpsu 76 1 Following follow VBG cord-261579-f0prnpsu 76 2 cell cell NN cord-261579-f0prnpsu 76 3 lysis lysis NN cord-261579-f0prnpsu 76 4 and and CC cord-261579-f0prnpsu 76 5 immunoprecipitation immunoprecipitation NN cord-261579-f0prnpsu 76 6 using use VBG cord-261579-f0prnpsu 76 7 α α NN cord-261579-f0prnpsu 76 8 - - HYPH cord-261579-f0prnpsu 76 9 GP-2 GP-2 NNP cord-261579-f0prnpsu 76 10 , , , cord-261579-f0prnpsu 76 11 samples sample NNS cord-261579-f0prnpsu 76 12 were be VBD cord-261579-f0prnpsu 76 13 subjected subject VBN cord-261579-f0prnpsu 76 14 to to IN cord-261579-f0prnpsu 76 15 SDS SDS NNP cord-261579-f0prnpsu 76 16 - - HYPH cord-261579-f0prnpsu 76 17 PAGE PAGE NNP cord-261579-f0prnpsu 76 18 and and CC cord-261579-f0prnpsu 76 19 blotted blot VBD cord-261579-f0prnpsu 76 20 to to IN cord-261579-f0prnpsu 76 21 nitrocellulose nitrocellulose NN cord-261579-f0prnpsu 76 22 . . . cord-261579-f0prnpsu 77 1 Surface surface NN cord-261579-f0prnpsu 77 2 biotin biotin NN cord-261579-f0prnpsu 77 3 - - HYPH cord-261579-f0prnpsu 77 4 labelled label VBN cord-261579-f0prnpsu 77 5 proteins protein NNS cord-261579-f0prnpsu 77 6 were be VBD cord-261579-f0prnpsu 77 7 visualized visualize VBN cord-261579-f0prnpsu 77 8 with with IN cord-261579-f0prnpsu 77 9 streptavidin streptavidin NN cord-261579-f0prnpsu 77 10 - - HYPH cord-261579-f0prnpsu 77 11 peroxidase peroxidase NN cord-261579-f0prnpsu 77 12 and and CC cord-261579-f0prnpsu 77 13 chemiluminescence chemiluminescence NN cord-261579-f0prnpsu 77 14 . . . cord-261579-f0prnpsu 78 1 LCMV LCMV NNP cord-261579-f0prnpsu 78 2 and and CC cord-261579-f0prnpsu 78 3 Lassa Lassa NNP cord-261579-f0prnpsu 78 4 virus virus NN cord-261579-f0prnpsu 78 5 GP GP NNP cord-261579-f0prnpsu 78 6 - - HYPH cord-261579-f0prnpsu 78 7 C C NNP cord-261579-f0prnpsu 78 8 are be VBP cord-261579-f0prnpsu 78 9 cleaved cleave VBN cord-261579-f0prnpsu 78 10 by by IN cord-261579-f0prnpsu 78 11 the the DT cord-261579-f0prnpsu 78 12 same same JJ cord-261579-f0prnpsu 78 13 protease protease NN cord-261579-f0prnpsu 78 14 , , , cord-261579-f0prnpsu 78 15 SKI-1 SKI-1 NNP cord-261579-f0prnpsu 78 16 / / SYM cord-261579-f0prnpsu 78 17 S1P s1p NN cord-261579-f0prnpsu 78 18 , , , cord-261579-f0prnpsu 78 19 although although IN cord-261579-f0prnpsu 78 20 proteolytic proteolytic JJ cord-261579-f0prnpsu 78 21 processing processing NN cord-261579-f0prnpsu 78 22 of of IN cord-261579-f0prnpsu 78 23 the the DT cord-261579-f0prnpsu 78 24 GPs gps NNS cord-261579-f0prnpsu 78 25 occurs occur VBZ cord-261579-f0prnpsu 78 26 in in IN cord-261579-f0prnpsu 78 27 different different JJ cord-261579-f0prnpsu 78 28 subcellular subcellular JJ cord-261579-f0prnpsu 78 29 compartments compartment NNS cord-261579-f0prnpsu 78 30 . . . cord-261579-f0prnpsu 79 1 Lassa Lassa NNP cord-261579-f0prnpsu 79 2 virus virus NN cord-261579-f0prnpsu 79 3 GP GP NNP cord-261579-f0prnpsu 79 4 - - HYPH cord-261579-f0prnpsu 79 5 C C NNP cord-261579-f0prnpsu 79 6 is be VBZ cord-261579-f0prnpsu 79 7 cleaved cleave VBN cord-261579-f0prnpsu 79 8 early early RB cord-261579-f0prnpsu 79 9 along along IN cord-261579-f0prnpsu 79 10 the the DT cord-261579-f0prnpsu 79 11 secretory secretory JJ cord-261579-f0prnpsu 79 12 pathway pathway NN cord-261579-f0prnpsu 79 13 in in IN cord-261579-f0prnpsu 79 14 the the DT cord-261579-f0prnpsu 79 15 ER ER NNP cord-261579-f0prnpsu 79 16 or or CC cord-261579-f0prnpsu 79 17 an an DT cord-261579-f0prnpsu 79 18 early early JJ cord-261579-f0prnpsu 79 19 Golgi Golgi NNP cord-261579-f0prnpsu 79 20 stack stack NN cord-261579-f0prnpsu 79 21 , , , cord-261579-f0prnpsu 79 22 whereas whereas IN cord-261579-f0prnpsu 79 23 LCMV LCMV NNP cord-261579-f0prnpsu 79 24 GP GP NNP cord-261579-f0prnpsu 79 25 - - HYPH cord-261579-f0prnpsu 79 26 C C NNP cord-261579-f0prnpsu 79 27 is be VBZ cord-261579-f0prnpsu 79 28 cleaved cleave VBN cord-261579-f0prnpsu 79 29 in in IN cord-261579-f0prnpsu 79 30 the the DT cord-261579-f0prnpsu 79 31 Golgi Golgi NNP cord-261579-f0prnpsu 79 32 or or CC cord-261579-f0prnpsu 79 33 post post JJ cord-261579-f0prnpsu 79 34 - - JJ cord-261579-f0prnpsu 79 35 Golgi golgi JJ cord-261579-f0prnpsu 79 36 compartment compartment NN cord-261579-f0prnpsu 79 37 [ [ -LRB- cord-261579-f0prnpsu 79 38 6 6 CD cord-261579-f0prnpsu 79 39 , , , cord-261579-f0prnpsu 79 40 10 10 CD cord-261579-f0prnpsu 79 41 , , , cord-261579-f0prnpsu 79 42 22 22 CD cord-261579-f0prnpsu 79 43 ] ] -RRB- cord-261579-f0prnpsu 79 44 . . . cord-261579-f0prnpsu 80 1 Furthermore furthermore RB cord-261579-f0prnpsu 80 2 , , , cord-261579-f0prnpsu 80 3 for for IN cord-261579-f0prnpsu 80 4 LCMV LCMV NNP cord-261579-f0prnpsu 80 5 GP GP NNP cord-261579-f0prnpsu 80 6 - - HYPH cord-261579-f0prnpsu 80 7 C C NNP cord-261579-f0prnpsu 80 8 it -PRON- PRP cord-261579-f0prnpsu 80 9 has have VBZ cord-261579-f0prnpsu 80 10 been be VBN cord-261579-f0prnpsu 80 11 shown show VBN cord-261579-f0prnpsu 80 12 that that DT cord-261579-f0prnpsu 80 13 transition transition NN cord-261579-f0prnpsu 80 14 of of IN cord-261579-f0prnpsu 80 15 mannose mannose NN cord-261579-f0prnpsu 80 16 - - HYPH cord-261579-f0prnpsu 80 17 rich rich JJ cord-261579-f0prnpsu 80 18 carbohydrates carbohydrate NNS cord-261579-f0prnpsu 80 19 to to IN cord-261579-f0prnpsu 80 20 complex complex JJ cord-261579-f0prnpsu 80 21 carbohydrates carbohydrate NNS cord-261579-f0prnpsu 80 22 occurred occur VBD cord-261579-f0prnpsu 80 23 before before IN cord-261579-f0prnpsu 80 24 maturation maturation NN cord-261579-f0prnpsu 80 25 cleavage cleavage NN cord-261579-f0prnpsu 80 26 [ [ -LRB- cord-261579-f0prnpsu 80 27 22 22 CD cord-261579-f0prnpsu 80 28 ] ] -RRB- cord-261579-f0prnpsu 80 29 . . . cord-261579-f0prnpsu 81 1 In in IN cord-261579-f0prnpsu 81 2 order order NN cord-261579-f0prnpsu 81 3 to to TO cord-261579-f0prnpsu 81 4 analyse analyse VB cord-261579-f0prnpsu 81 5 the the DT cord-261579-f0prnpsu 81 6 glycosylation glycosylation NN cord-261579-f0prnpsu 81 7 status status NN cord-261579-f0prnpsu 81 8 of of IN cord-261579-f0prnpsu 81 9 Lassa Lassa NNP cord-261579-f0prnpsu 81 10 virus virus NN cord-261579-f0prnpsu 81 11 glycoproteins glycoprotein NNS cord-261579-f0prnpsu 81 12 on on IN cord-261579-f0prnpsu 81 13 the the DT cord-261579-f0prnpsu 81 14 outer outer JJ cord-261579-f0prnpsu 81 15 cell cell NN cord-261579-f0prnpsu 81 16 membrane membrane NN cord-261579-f0prnpsu 81 17 , , , cord-261579-f0prnpsu 81 18 cell cell NN cord-261579-f0prnpsu 81 19 surface surface NN cord-261579-f0prnpsu 81 20 biotinylation biotinylation NN cord-261579-f0prnpsu 81 21 was be VBD cord-261579-f0prnpsu 81 22 combined combine VBN cord-261579-f0prnpsu 81 23 with with IN cord-261579-f0prnpsu 81 24 a a DT cord-261579-f0prnpsu 81 25 subsequent subsequent JJ cord-261579-f0prnpsu 81 26 detachment detachment NN cord-261579-f0prnpsu 81 27 of of IN cord-261579-f0prnpsu 81 28 mannose mannose NN cord-261579-f0prnpsu 81 29 - - HYPH cord-261579-f0prnpsu 81 30 rich rich JJ cord-261579-f0prnpsu 81 31 N n CD cord-261579-f0prnpsu 81 32 - - HYPH cord-261579-f0prnpsu 81 33 glycans glycan NNS cord-261579-f0prnpsu 81 34 by by IN cord-261579-f0prnpsu 81 35 digestion digestion NN cord-261579-f0prnpsu 81 36 with with IN cord-261579-f0prnpsu 81 37 endo endo NN cord-261579-f0prnpsu 81 38 H h NN cord-261579-f0prnpsu 81 39 or or CC cord-261579-f0prnpsu 81 40 removal removal NN cord-261579-f0prnpsu 81 41 of of IN cord-261579-f0prnpsu 81 42 complex complex JJ cord-261579-f0prnpsu 81 43 N n CD cord-261579-f0prnpsu 81 44 - - HYPH cord-261579-f0prnpsu 81 45 glycans glycan NNS cord-261579-f0prnpsu 81 46 using use VBG cord-261579-f0prnpsu 81 47 PNGase PNGase NNP cord-261579-f0prnpsu 81 48 F F NNP cord-261579-f0prnpsu 81 49 ( ( -LRB- cord-261579-f0prnpsu 81 50 Fig Fig NNP cord-261579-f0prnpsu 81 51 . . NNP cord-261579-f0prnpsu 81 52 4 4 CD cord-261579-f0prnpsu 81 53 ) ) -RRB- cord-261579-f0prnpsu 81 54 . . . cord-261579-f0prnpsu 82 1 The the DT cord-261579-f0prnpsu 82 2 GP-2 GP-2 NNP cord-261579-f0prnpsu 82 3 subunit subunit NN cord-261579-f0prnpsu 82 4 is be VBZ cord-261579-f0prnpsu 82 5 detectable detectable JJ cord-261579-f0prnpsu 82 6 at at IN cord-261579-f0prnpsu 82 7 the the DT cord-261579-f0prnpsu 82 8 cell cell NN cord-261579-f0prnpsu 82 9 surface surface NN cord-261579-f0prnpsu 82 10 and and CC cord-261579-f0prnpsu 82 11 displays display VBZ cord-261579-f0prnpsu 82 12 partial partial JJ cord-261579-f0prnpsu 82 13 endo endo NN cord-261579-f0prnpsu 82 14 H h NN cord-261579-f0prnpsu 82 15 - - HYPH cord-261579-f0prnpsu 82 16 resistance resistance NN cord-261579-f0prnpsu 82 17 confirming confirm VBG cord-261579-f0prnpsu 82 18 a a DT cord-261579-f0prnpsu 82 19 modification modification NN cord-261579-f0prnpsu 82 20 of of IN cord-261579-f0prnpsu 82 21 its -PRON- PRP$ cord-261579-f0prnpsu 82 22 carbohydrate carbohydrate NN cord-261579-f0prnpsu 82 23 decoration decoration NN cord-261579-f0prnpsu 82 24 during during IN cord-261579-f0prnpsu 82 25 glycoprotein glycoprotein NN cord-261579-f0prnpsu 82 26 transport transport NN cord-261579-f0prnpsu 82 27 to to IN cord-261579-f0prnpsu 82 28 the the DT cord-261579-f0prnpsu 82 29 cell cell NN cord-261579-f0prnpsu 82 30 surface surface NN cord-261579-f0prnpsu 82 31 ( ( -LRB- cord-261579-f0prnpsu 82 32 lane lane NN cord-261579-f0prnpsu 82 33 2 2 CD cord-261579-f0prnpsu 82 34 ) ) -RRB- cord-261579-f0prnpsu 82 35 . . . cord-261579-f0prnpsu 83 1 Interestingly interestingly RB cord-261579-f0prnpsu 83 2 , , , cord-261579-f0prnpsu 83 3 the the DT cord-261579-f0prnpsu 83 4 glycoprotein glycoprotein NN cord-261579-f0prnpsu 83 5 precursor precursor NN cord-261579-f0prnpsu 83 6 GP gp NN cord-261579-f0prnpsu 83 7 - - HYPH cord-261579-f0prnpsu 83 8 C C NNP cord-261579-f0prnpsu 83 9 which which WDT cord-261579-f0prnpsu 83 10 is be VBZ cord-261579-f0prnpsu 83 11 transported transport VBN cord-261579-f0prnpsu 83 12 to to IN cord-261579-f0prnpsu 83 13 the the DT cord-261579-f0prnpsu 83 14 cell cell NN cord-261579-f0prnpsu 83 15 surface surface NN cord-261579-f0prnpsu 83 16 remains remain VBZ cord-261579-f0prnpsu 83 17 completely completely RB cord-261579-f0prnpsu 83 18 endo endo NN cord-261579-f0prnpsu 83 19 H h NN cord-261579-f0prnpsu 83 20 - - HYPH cord-261579-f0prnpsu 83 21 sensitive sensitive JJ cord-261579-f0prnpsu 83 22 suggesting suggest VBG cord-261579-f0prnpsu 83 23 that that IN cord-261579-f0prnpsu 83 24 cleavage cleavage NN cord-261579-f0prnpsu 83 25 might may MD cord-261579-f0prnpsu 83 26 be be VB cord-261579-f0prnpsu 83 27 a a DT cord-261579-f0prnpsu 83 28 prerequisite prerequisite NN cord-261579-f0prnpsu 83 29 for for IN cord-261579-f0prnpsu 83 30 further further JJ cord-261579-f0prnpsu 83 31 complex complex JJ cord-261579-f0prnpsu 83 32 glycosylation glycosylation NN cord-261579-f0prnpsu 83 33 . . . cord-261579-f0prnpsu 84 1 In in IN cord-261579-f0prnpsu 84 2 addition addition NN cord-261579-f0prnpsu 84 3 , , , cord-261579-f0prnpsu 84 4 uncleaved uncleaved JJ cord-261579-f0prnpsu 84 5 endo endo NN cord-261579-f0prnpsu 84 6 Hsensitive Hsensitive NNP cord-261579-f0prnpsu 84 7 Lassa Lassa NNP cord-261579-f0prnpsu 84 8 virus virus NN cord-261579-f0prnpsu 84 9 GP gp NN cord-261579-f0prnpsu 84 10 - - HYPH cord-261579-f0prnpsu 84 11 C C NNP cord-261579-f0prnpsu 84 12 is be VBZ cord-261579-f0prnpsu 84 13 also also RB cord-261579-f0prnpsu 84 14 transported transport VBN cord-261579-f0prnpsu 84 15 to to IN cord-261579-f0prnpsu 84 16 the the DT cord-261579-f0prnpsu 84 17 cell cell NN cord-261579-f0prnpsu 84 18 surface surface NN cord-261579-f0prnpsu 84 19 of of IN cord-261579-f0prnpsu 84 20 SKI-1 SKI-1 NNP cord-261579-f0prnpsu 84 21 / / SYM cord-261579-f0prnpsu 84 22 S1P s1p NN cord-261579-f0prnpsu 84 23 deficient deficient JJ cord-261579-f0prnpsu 84 24 cells cell NNS cord-261579-f0prnpsu 84 25 ( ( -LRB- cord-261579-f0prnpsu 84 26 data datum NNS cord-261579-f0prnpsu 84 27 not not RB cord-261579-f0prnpsu 84 28 shown show VBN cord-261579-f0prnpsu 84 29 ) ) -RRB- cord-261579-f0prnpsu 84 30 . . . cord-261579-f0prnpsu 85 1 The the DT cord-261579-f0prnpsu 85 2 finding finding NN cord-261579-f0prnpsu 85 3 that that IN cord-261579-f0prnpsu 85 4 only only RB cord-261579-f0prnpsu 85 5 an an DT cord-261579-f0prnpsu 85 6 endo endo NN cord-261579-f0prnpsu 85 7 H h NN cord-261579-f0prnpsu 85 8 - - HYPH cord-261579-f0prnpsu 85 9 sensitive sensitive JJ cord-261579-f0prnpsu 85 10 form form NN cord-261579-f0prnpsu 85 11 of of IN cord-261579-f0prnpsu 85 12 Lassa Lassa NNP cord-261579-f0prnpsu 85 13 virus virus NN cord-261579-f0prnpsu 85 14 GP GP NNP cord-261579-f0prnpsu 85 15 - - HYPH cord-261579-f0prnpsu 85 16 C C NNP cord-261579-f0prnpsu 85 17 was be VBD cord-261579-f0prnpsu 85 18 found find VBN cord-261579-f0prnpsu 85 19 at at IN cord-261579-f0prnpsu 85 20 the the DT cord-261579-f0prnpsu 85 21 cell cell NN cord-261579-f0prnpsu 85 22 surface surface NN cord-261579-f0prnpsu 85 23 suggests suggest VBZ cord-261579-f0prnpsu 85 24 that that IN cord-261579-f0prnpsu 85 25 partial partial JJ cord-261579-f0prnpsu 85 26 endo endo NN cord-261579-f0prnpsu 85 27 H h NN cord-261579-f0prnpsu 85 28 - - HYPH cord-261579-f0prnpsu 85 29 resistance resistance NN cord-261579-f0prnpsu 85 30 of of IN cord-261579-f0prnpsu 85 31 the the DT cord-261579-f0prnpsu 85 32 Lassa Lassa NNP cord-261579-f0prnpsu 85 33 virus virus NN cord-261579-f0prnpsu 85 34 glycoprotein glycoprotein NN cord-261579-f0prnpsu 85 35 is be VBZ cord-261579-f0prnpsu 85 36 only only RB cord-261579-f0prnpsu 85 37 acquired acquire VBN cord-261579-f0prnpsu 85 38 when when WRB cord-261579-f0prnpsu 85 39 the the DT cord-261579-f0prnpsu 85 40 glycoprotein glycoprotein NN cord-261579-f0prnpsu 85 41 is be VBZ cord-261579-f0prnpsu 85 42 cleaved cleave VBN cord-261579-f0prnpsu 85 43 into into IN cord-261579-f0prnpsu 85 44 its -PRON- PRP$ cord-261579-f0prnpsu 85 45 subunits subunit NNS cord-261579-f0prnpsu 85 46 . . . cord-261579-f0prnpsu 86 1 This this DT cord-261579-f0prnpsu 86 2 observation observation NN cord-261579-f0prnpsu 86 3 is be VBZ cord-261579-f0prnpsu 86 4 in in IN cord-261579-f0prnpsu 86 5 contrast contrast NN cord-261579-f0prnpsu 86 6 to to IN cord-261579-f0prnpsu 86 7 findings finding NNS cord-261579-f0prnpsu 86 8 for for IN cord-261579-f0prnpsu 86 9 the the DT cord-261579-f0prnpsu 86 10 LCMV LCMV NNP cord-261579-f0prnpsu 86 11 glycoprotein glycoprotein NN cord-261579-f0prnpsu 86 12 where where WRB cord-261579-f0prnpsu 86 13 an an DT cord-261579-f0prnpsu 86 14 endo endo NN cord-261579-f0prnpsu 86 15 H h NN cord-261579-f0prnpsu 86 16 - - HYPH cord-261579-f0prnpsu 86 17 resistant resistant JJ cord-261579-f0prnpsu 86 18 form form NN cord-261579-f0prnpsu 86 19 of of IN cord-261579-f0prnpsu 86 20 GP GP NNP cord-261579-f0prnpsu 86 21 - - HYPH cord-261579-f0prnpsu 86 22 C C NNP cord-261579-f0prnpsu 86 23 was be VBD cord-261579-f0prnpsu 86 24 described describe VBN cord-261579-f0prnpsu 86 25 [ [ -LRB- cord-261579-f0prnpsu 86 26 22 22 CD cord-261579-f0prnpsu 86 27 ] ] -RRB- cord-261579-f0prnpsu 86 28 . . . cord-261579-f0prnpsu 87 1 However however RB cord-261579-f0prnpsu 87 2 , , , cord-261579-f0prnpsu 87 3 since since IN cord-261579-f0prnpsu 87 4 cleavage cleavage NN cord-261579-f0prnpsu 87 5 of of IN cord-261579-f0prnpsu 87 6 Lassa Lassa NNP cord-261579-f0prnpsu 87 7 virus virus NN cord-261579-f0prnpsu 87 8 GP gp NN cord-261579-f0prnpsu 87 9 - - HYPH cord-261579-f0prnpsu 87 10 C C NNP cord-261579-f0prnpsu 87 11 by by IN cord-261579-f0prnpsu 87 12 SKI-1 SKI-1 NNP cord-261579-f0prnpsu 87 13 / / SYM cord-261579-f0prnpsu 87 14 S1P s1p NN cord-261579-f0prnpsu 87 15 occurs occur VBZ cord-261579-f0prnpsu 87 16 earlier early RBR cord-261579-f0prnpsu 87 17 in in IN cord-261579-f0prnpsu 87 18 the the DT cord-261579-f0prnpsu 87 19 exocytotic exocytotic JJ cord-261579-f0prnpsu 87 20 pathway pathway NN cord-261579-f0prnpsu 87 21 than than IN cord-261579-f0prnpsu 87 22 LCMV LCMV NNP cord-261579-f0prnpsu 87 23 GP GP NNP cord-261579-f0prnpsu 87 24 - - HYPH cord-261579-f0prnpsu 87 25 C C NNP cord-261579-f0prnpsu 87 26 [ [ -LRB- cord-261579-f0prnpsu 87 27 6 6 CD cord-261579-f0prnpsu 87 28 , , , cord-261579-f0prnpsu 87 29 10 10 CD cord-261579-f0prnpsu 87 30 , , , cord-261579-f0prnpsu 87 31 22 22 CD cord-261579-f0prnpsu 87 32 ] ] -RRB- cord-261579-f0prnpsu 87 33 , , , cord-261579-f0prnpsu 87 34 cleavage cleavage NN cord-261579-f0prnpsu 87 35 of of IN cord-261579-f0prnpsu 87 36 Lassa Lassa NNP cord-261579-f0prnpsu 87 37 virus virus NN cord-261579-f0prnpsu 87 38 GP gp NN cord-261579-f0prnpsu 87 39 - - HYPH cord-261579-f0prnpsu 87 40 C C NNP cord-261579-f0prnpsu 87 41 occurs occur VBZ cord-261579-f0prnpsu 87 42 prior prior RB cord-261579-f0prnpsu 87 43 to to IN cord-261579-f0prnpsu 87 44 trimming trimming NN cord-261579-f0prnpsu 87 45 of of IN cord-261579-f0prnpsu 87 46 the the DT cord-261579-f0prnpsu 87 47 mannose mannose NN cord-261579-f0prnpsu 87 48 - - HYPH cord-261579-f0prnpsu 87 49 rich rich JJ cord-261579-f0prnpsu 87 50 N n CD cord-261579-f0prnpsu 87 51 - - HYPH cord-261579-f0prnpsu 87 52 glycans glycan NNS cord-261579-f0prnpsu 87 53 to to IN cord-261579-f0prnpsu 87 54 complex complex JJ cord-261579-f0prnpsu 87 55 sugar sugar NN cord-261579-f0prnpsu 87 56 types type NNS cord-261579-f0prnpsu 87 57 . . . cord-261579-f0prnpsu 88 1 It -PRON- PRP cord-261579-f0prnpsu 88 2 can can MD cord-261579-f0prnpsu 88 3 not not RB cord-261579-f0prnpsu 88 4 be be VB cord-261579-f0prnpsu 88 5 ruled rule VBN cord-261579-f0prnpsu 88 6 out out RP cord-261579-f0prnpsu 88 7 that that IN cord-261579-f0prnpsu 88 8 the the DT cord-261579-f0prnpsu 88 9 use use NN cord-261579-f0prnpsu 88 10 of of IN cord-261579-f0prnpsu 88 11 glycosylation glycosylation NN cord-261579-f0prnpsu 88 12 sites site NNS cord-261579-f0prnpsu 88 13 and and CC cord-261579-f0prnpsu 88 14 the the DT cord-261579-f0prnpsu 88 15 N n CD cord-261579-f0prnpsu 88 16 - - HYPH cord-261579-f0prnpsu 88 17 glycan glycan NN cord-261579-f0prnpsu 88 18 trimming trimming NN cord-261579-f0prnpsu 88 19 differs differ VBZ cord-261579-f0prnpsu 88 20 between between IN cord-261579-f0prnpsu 88 21 recombinant recombinant JJ cord-261579-f0prnpsu 88 22 expressed express VBD cord-261579-f0prnpsu 88 23 glycoproteins glycoprotein NNS cord-261579-f0prnpsu 88 24 and and CC cord-261579-f0prnpsu 88 25 GP gp NN cord-261579-f0prnpsu 88 26 in in IN cord-261579-f0prnpsu 88 27 LASV LASV NNP cord-261579-f0prnpsu 88 28 - - HYPH cord-261579-f0prnpsu 88 29 infected infect VBN cord-261579-f0prnpsu 88 30 cells cell NNS cord-261579-f0prnpsu 88 31 . . . cord-261579-f0prnpsu 89 1 It -PRON- PRP cord-261579-f0prnpsu 89 2 was be VBD cord-261579-f0prnpsu 89 3 shown show VBN cord-261579-f0prnpsu 89 4 recently recently RB cord-261579-f0prnpsu 89 5 that that IN cord-261579-f0prnpsu 89 6 recombinant recombinant NN cord-261579-f0prnpsu 89 7 expressed express VBD cord-261579-f0prnpsu 89 8 M M NNP cord-261579-f0prnpsu 89 9 protein protein NN cord-261579-f0prnpsu 89 10 of of IN cord-261579-f0prnpsu 89 11 SARS SARS NNP cord-261579-f0prnpsu 89 12 - - HYPH cord-261579-f0prnpsu 89 13 CoV CoV NNP cord-261579-f0prnpsu 89 14 gained gain VBD cord-261579-f0prnpsu 89 15 complex complex JJ cord-261579-f0prnpsu 89 16 - - HYPH cord-261579-f0prnpsu 89 17 type type NN cord-261579-f0prnpsu 89 18 N n NN cord-261579-f0prnpsu 89 19 - - HYPH cord-261579-f0prnpsu 89 20 glycosylation glycosylation NN cord-261579-f0prnpsu 89 21 whereas whereas RB cord-261579-f0prnpsu 89 22 in in IN cord-261579-f0prnpsu 89 23 SARS SARS NNP cord-261579-f0prnpsu 89 24 - - HYPH cord-261579-f0prnpsu 89 25 CoV CoV NNP cord-261579-f0prnpsu 89 26 infected infect VBD cord-261579-f0prnpsu 89 27 cells cell NNS cord-261579-f0prnpsu 89 28 N n CD cord-261579-f0prnpsu 89 29 - - HYPH cord-261579-f0prnpsu 89 30 glycosylation glycosylation NN cord-261579-f0prnpsu 89 31 of of IN cord-261579-f0prnpsu 89 32 M M NNP cord-261579-f0prnpsu 89 33 remained remain VBD cord-261579-f0prnpsu 89 34 endo endo NN cord-261579-f0prnpsu 89 35 H h NN cord-261579-f0prnpsu 89 36 - - HYPH cord-261579-f0prnpsu 89 37 sensitive sensitive JJ cord-261579-f0prnpsu 89 38 [ [ -LRB- cord-261579-f0prnpsu 89 39 25 25 CD cord-261579-f0prnpsu 89 40 ] ] -RRB- cord-261579-f0prnpsu 89 41 . . . cord-261579-f0prnpsu 90 1 However however RB cord-261579-f0prnpsu 90 2 , , , cord-261579-f0prnpsu 90 3 in in IN cord-261579-f0prnpsu 90 4 general general JJ cord-261579-f0prnpsu 90 5 a a DT cord-261579-f0prnpsu 90 6 good good JJ cord-261579-f0prnpsu 90 7 correlation correlation NN cord-261579-f0prnpsu 90 8 between between IN cord-261579-f0prnpsu 90 9 results result NNS cord-261579-f0prnpsu 90 10 obtained obtain VBN cord-261579-f0prnpsu 90 11 after after IN cord-261579-f0prnpsu 90 12 solitary solitary JJ cord-261579-f0prnpsu 90 13 expression expression NN cord-261579-f0prnpsu 90 14 and and CC cord-261579-f0prnpsu 90 15 studies study NNS cord-261579-f0prnpsu 90 16 using use VBG cord-261579-f0prnpsu 90 17 the the DT cord-261579-f0prnpsu 90 18 homologues homologue NNS cord-261579-f0prnpsu 90 19 viral viral JJ cord-261579-f0prnpsu 90 20 system system NN cord-261579-f0prnpsu 90 21 have have VBP cord-261579-f0prnpsu 90 22 been be VBN cord-261579-f0prnpsu 90 23 observed observe VBN cord-261579-f0prnpsu 90 24 . . . cord-261579-f0prnpsu 91 1 To to TO cord-261579-f0prnpsu 91 2 address address VB cord-261579-f0prnpsu 91 3 this this DT cord-261579-f0prnpsu 91 4 kind kind NN cord-261579-f0prnpsu 91 5 of of IN cord-261579-f0prnpsu 91 6 issues issue NNS cord-261579-f0prnpsu 91 7 , , , cord-261579-f0prnpsu 91 8 the the DT cord-261579-f0prnpsu 91 9 development development NN cord-261579-f0prnpsu 91 10 of of IN cord-261579-f0prnpsu 91 11 a a DT cord-261579-f0prnpsu 91 12 " " `` cord-261579-f0prnpsu 91 13 reverse reverse JJ cord-261579-f0prnpsu 91 14 genetic genetic JJ cord-261579-f0prnpsu 91 15 system system NN cord-261579-f0prnpsu 91 16 " " '' cord-261579-f0prnpsu 91 17 for for IN cord-261579-f0prnpsu 91 18 LASV LASV NNP cord-261579-f0prnpsu 91 19 will will MD cord-261579-f0prnpsu 91 20 be be VB cord-261579-f0prnpsu 91 21 necessary necessary JJ cord-261579-f0prnpsu 91 22 that that WDT cord-261579-f0prnpsu 91 23 would would MD cord-261579-f0prnpsu 91 24 allow allow VB cord-261579-f0prnpsu 91 25 analysing analyse VBG cord-261579-f0prnpsu 91 26 the the DT cord-261579-f0prnpsu 91 27 role role NN cord-261579-f0prnpsu 91 28 of of IN cord-261579-f0prnpsu 91 29 glycosylation glycosylation NN cord-261579-f0prnpsu 91 30 of of IN cord-261579-f0prnpsu 91 31 GP gp NN cord-261579-f0prnpsu 91 32 in in IN cord-261579-f0prnpsu 91 33 the the DT cord-261579-f0prnpsu 91 34 correct correct JJ cord-261579-f0prnpsu 91 35 viral viral JJ cord-261579-f0prnpsu 91 36 context context NN cord-261579-f0prnpsu 91 37 . . . cord-261579-f0prnpsu 92 1 Taken take VBN cord-261579-f0prnpsu 92 2 together together RB cord-261579-f0prnpsu 92 3 , , , cord-261579-f0prnpsu 92 4 our -PRON- PRP$ cord-261579-f0prnpsu 92 5 study study NN cord-261579-f0prnpsu 92 6 suggest suggest VBP cord-261579-f0prnpsu 92 7 that that IN cord-261579-f0prnpsu 92 8 individual individual JJ cord-261579-f0prnpsu 92 9 N n CD cord-261579-f0prnpsu 92 10 - - HYPH cord-261579-f0prnpsu 92 11 linked link VBN cord-261579-f0prnpsu 92 12 oligosaccharides oligosaccharide NNS cord-261579-f0prnpsu 92 13 of of IN cord-261579-f0prnpsu 92 14 the the DT cord-261579-f0prnpsu 92 15 Lassa Lassa NNP cord-261579-f0prnpsu 92 16 virus virus NN cord-261579-f0prnpsu 92 17 glycoprotein glycoprotein NN cord-261579-f0prnpsu 92 18 differ differ VBP cord-261579-f0prnpsu 92 19 greatly greatly RB cord-261579-f0prnpsu 92 20 in in IN cord-261579-f0prnpsu 92 21 terms term NNS cord-261579-f0prnpsu 92 22 of of IN cord-261579-f0prnpsu 92 23 their -PRON- PRP$ cord-261579-f0prnpsu 92 24 importance importance NN cord-261579-f0prnpsu 92 25 for for IN cord-261579-f0prnpsu 92 26 correct correct JJ cord-261579-f0prnpsu 92 27 protein protein NN cord-261579-f0prnpsu 92 28 folding folding NN cord-261579-f0prnpsu 92 29 which which WDT cord-261579-f0prnpsu 92 30 seems seem VBZ cord-261579-f0prnpsu 92 31 to to TO cord-261579-f0prnpsu 92 32 be be VB cord-261579-f0prnpsu 92 33 important important JJ cord-261579-f0prnpsu 92 34 for for IN cord-261579-f0prnpsu 92 35 activation activation NN cord-261579-f0prnpsu 92 36 cleavage cleavage NN cord-261579-f0prnpsu 92 37 by by IN cord-261579-f0prnpsu 92 38 SKI-1/ ski-1/ NN cord-261579-f0prnpsu 93 1 S1P. s1p. RB cord-261579-f0prnpsu 94 1 The the DT cord-261579-f0prnpsu 94 2 differences difference NNS cord-261579-f0prnpsu 94 3 between between IN cord-261579-f0prnpsu 94 4 the the DT cord-261579-f0prnpsu 94 5 precursor precursor NN cord-261579-f0prnpsu 94 6 glycoprotein glycoprotein NN cord-261579-f0prnpsu 94 7 GP GP NNP cord-261579-f0prnpsu 94 8 - - HYPH cord-261579-f0prnpsu 94 9 C C NNP cord-261579-f0prnpsu 94 10 and and CC cord-261579-f0prnpsu 94 11 the the DT cord-261579-f0prnpsu 94 12 cleaved cleaved JJ cord-261579-f0prnpsu 94 13 subunits subunit NNS cord-261579-f0prnpsu 94 14 GP-1 GP-1 NNP cord-261579-f0prnpsu 94 15 and and CC cord-261579-f0prnpsu 94 16 GP-2 GP-2 NNP cord-261579-f0prnpsu 94 17 , , , cord-261579-f0prnpsu 94 18 not not RB cord-261579-f0prnpsu 94 19 only only RB cord-261579-f0prnpsu 94 20 with with IN cord-261579-f0prnpsu 94 21 respect respect NN cord-261579-f0prnpsu 94 22 to to IN cord-261579-f0prnpsu 94 23 N n NN cord-261579-f0prnpsu 94 24 - - HYPH cord-261579-f0prnpsu 94 25 glycosylation glycosylation NN cord-261579-f0prnpsu 94 26 , , , cord-261579-f0prnpsu 94 27 remain remain VBP cord-261579-f0prnpsu 94 28 an an DT cord-261579-f0prnpsu 94 29 enigma enigma NN cord-261579-f0prnpsu 94 30 since since IN cord-261579-f0prnpsu 94 31 only only RB cord-261579-f0prnpsu 94 32 the the DT cord-261579-f0prnpsu 94 33 cleaved cleaved JJ cord-261579-f0prnpsu 94 34 glycoprotein glycoprotein NN cord-261579-f0prnpsu 94 35 subunits subunit NNS cord-261579-f0prnpsu 94 36 are be VBP cord-261579-f0prnpsu 94 37 incorporated incorporate VBN cord-261579-f0prnpsu 94 38 into into IN cord-261579-f0prnpsu 94 39 virus virus NN cord-261579-f0prnpsu 94 40 particles particle NNS cord-261579-f0prnpsu 94 41 [ [ -LRB- cord-261579-f0prnpsu 94 42 6 6 CD cord-261579-f0prnpsu 94 43 ] ] -RRB- cord-261579-f0prnpsu 94 44 . . . cord-261579-f0prnpsu 94 45 _SP cord-261579-f0prnpsu 95 1 Lassa Lassa NNP cord-261579-f0prnpsu 95 2 fever fever NN cord-261579-f0prnpsu 96 1 Imported import VBN cord-261579-f0prnpsu 96 2 lassa lassa NNP cord-261579-f0prnpsu 96 3 fever fever NN cord-261579-f0prnpsu 96 4 in in IN cord-261579-f0prnpsu 96 5 Germany Germany NNP cord-261579-f0prnpsu 96 6 : : : cord-261579-f0prnpsu 97 1 molecular molecular JJ cord-261579-f0prnpsu 97 2 characterization characterization NN cord-261579-f0prnpsu 97 3 of of IN cord-261579-f0prnpsu 97 4 a a DT cord-261579-f0prnpsu 97 5 new new JJ cord-261579-f0prnpsu 97 6 lassa lassa NNP cord-261579-f0prnpsu 97 7 virus virus NN cord-261579-f0prnpsu 97 8 strain strain NN cord-261579-f0prnpsu 98 1 Lassa Lassa NNP cord-261579-f0prnpsu 98 2 virus virus NN cord-261579-f0prnpsu 98 3 Signal Signal NNP cord-261579-f0prnpsu 98 4 peptide peptide NN cord-261579-f0prnpsu 98 5 of of IN cord-261579-f0prnpsu 98 6 Lassa Lassa NNP cord-261579-f0prnpsu 98 7 virus virus NN cord-261579-f0prnpsu 98 8 glycoprotein glycoprotein NN cord-261579-f0prnpsu 98 9 GP GP NNP cord-261579-f0prnpsu 98 10 - - HYPH cord-261579-f0prnpsu 98 11 C C NNP cord-261579-f0prnpsu 98 12 exhibits exhibit VBZ cord-261579-f0prnpsu 98 13 an an DT cord-261579-f0prnpsu 98 14 unusual unusual JJ cord-261579-f0prnpsu 98 15 length length NN cord-261579-f0prnpsu 98 16 Identification identification NN cord-261579-f0prnpsu 98 17 of of IN cord-261579-f0prnpsu 98 18 Lassa Lassa NNP cord-261579-f0prnpsu 98 19 virus virus NN cord-261579-f0prnpsu 98 20 glycoprotein glycoprotein NN cord-261579-f0prnpsu 98 21 signal signal NN cord-261579-f0prnpsu 98 22 peptide peptide NN cord-261579-f0prnpsu 98 23 as as IN cord-261579-f0prnpsu 98 24 a a DT cord-261579-f0prnpsu 98 25 trans trans JJ cord-261579-f0prnpsu 98 26 - - JJ cord-261579-f0prnpsu 98 27 acting acting JJ cord-261579-f0prnpsu 98 28 maturation maturation NN cord-261579-f0prnpsu 98 29 factor factor NN cord-261579-f0prnpsu 99 1 The the DT cord-261579-f0prnpsu 99 2 Lassa Lassa NNP cord-261579-f0prnpsu 99 3 virus virus NN cord-261579-f0prnpsu 99 4 glycoprotein glycoprotein NN cord-261579-f0prnpsu 99 5 precursor precursor NN cord-261579-f0prnpsu 99 6 GP GP NNP cord-261579-f0prnpsu 99 7 - - HYPH cord-261579-f0prnpsu 99 8 C C NNP cord-261579-f0prnpsu 99 9 is be VBZ cord-261579-f0prnpsu 99 10 proteolytically proteolytically RB cord-261579-f0prnpsu 99 11 processed process VBN cord-261579-f0prnpsu 99 12 by by IN cord-261579-f0prnpsu 99 13 subtilase subtilase NNP cord-261579-f0prnpsu 100 1 SKI-1 SKI-1 NNP cord-261579-f0prnpsu 100 2 / / SYM cord-261579-f0prnpsu 100 3 S1P s1p NN cord-261579-f0prnpsu 100 4 Identification identification NN cord-261579-f0prnpsu 100 5 of of IN cord-261579-f0prnpsu 100 6 a a DT cord-261579-f0prnpsu 100 7 novel novel JJ cord-261579-f0prnpsu 100 8 consensus consensus NN cord-261579-f0prnpsu 100 9 sequence sequence NN cord-261579-f0prnpsu 100 10 at at IN cord-261579-f0prnpsu 100 11 the the DT cord-261579-f0prnpsu 100 12 cleavage cleavage NN cord-261579-f0prnpsu 100 13 site site NN cord-261579-f0prnpsu 100 14 of of IN cord-261579-f0prnpsu 100 15 the the DT cord-261579-f0prnpsu 100 16 Lassa Lassa NNP cord-261579-f0prnpsu 100 17 virus virus NN cord-261579-f0prnpsu 100 18 glycoprotein glycoprotein NN cord-261579-f0prnpsu 100 19 Characterization characterization NN cord-261579-f0prnpsu 100 20 of of IN cord-261579-f0prnpsu 100 21 the the DT cord-261579-f0prnpsu 100 22 glycoproteins glycoprotein NNS cord-261579-f0prnpsu 100 23 of of IN cord-261579-f0prnpsu 100 24 Crimean Crimean NNP cord-261579-f0prnpsu 100 25 - - HYPH cord-261579-f0prnpsu 100 26 Congo Congo NNP cord-261579-f0prnpsu 100 27 hemorrhagic hemorrhagic JJ cord-261579-f0prnpsu 100 28 fever fever NN cord-261579-f0prnpsu 100 29 virus virus NN cord-261579-f0prnpsu 100 30 Crimean Crimean NNP cord-261579-f0prnpsu 100 31 - - HYPH cord-261579-f0prnpsu 100 32 Congo Congo NNP cord-261579-f0prnpsu 100 33 hemorrhagic hemorrhagic JJ cord-261579-f0prnpsu 100 34 fever fever NN cord-261579-f0prnpsu 100 35 virus virus NN cord-261579-f0prnpsu 100 36 glycoprotein glycoprotein NN cord-261579-f0prnpsu 100 37 proteolytic proteolytic JJ cord-261579-f0prnpsu 100 38 processing processing NN cord-261579-f0prnpsu 100 39 by by IN cord-261579-f0prnpsu 100 40 subtilase subtilase NNP cord-261579-f0prnpsu 101 1 SKI-1 SKI-1 NNP cord-261579-f0prnpsu 101 2 Endoproteolytic endoproteolytic JJ cord-261579-f0prnpsu 101 3 processing processing NN cord-261579-f0prnpsu 101 4 of of IN cord-261579-f0prnpsu 101 5 the the DT cord-261579-f0prnpsu 101 6 lymphocytic lymphocytic JJ cord-261579-f0prnpsu 101 7 choriomeningitis choriomeningitis NNP cord-261579-f0prnpsu 101 8 virus virus NN cord-261579-f0prnpsu 101 9 glycoprotein glycoprotein NN cord-261579-f0prnpsu 101 10 by by IN cord-261579-f0prnpsu 101 11 the the DT cord-261579-f0prnpsu 101 12 subtilase subtilase NNP cord-261579-f0prnpsu 101 13 SKI-1 SKI-1 NNP cord-261579-f0prnpsu 101 14 / / SYM cord-261579-f0prnpsu 101 15 S1P S1P VBD cord-261579-f0prnpsu 101 16 Host host NN cord-261579-f0prnpsu 101 17 cell cell NN cord-261579-f0prnpsu 101 18 proteases protease NNS cord-261579-f0prnpsu 101 19 controlling control VBG cord-261579-f0prnpsu 101 20 virus virus NN cord-261579-f0prnpsu 101 21 pathogenicity pathogenicity NN cord-261579-f0prnpsu 101 22 Individual individual JJ cord-261579-f0prnpsu 101 23 roles role NNS cord-261579-f0prnpsu 101 24 of of IN cord-261579-f0prnpsu 101 25 N n NN cord-261579-f0prnpsu 101 26 - - HYPH cord-261579-f0prnpsu 101 27 linked link VBN cord-261579-f0prnpsu 101 28 oligosaccharide oligosaccharide NN cord-261579-f0prnpsu 101 29 chains chain NNS cord-261579-f0prnpsu 101 30 in in IN cord-261579-f0prnpsu 101 31 intracellular intracellular JJ cord-261579-f0prnpsu 101 32 transport transport NN cord-261579-f0prnpsu 101 33 of of IN cord-261579-f0prnpsu 101 34 the the DT cord-261579-f0prnpsu 101 35 paramyxovirus paramyxovirus NNP cord-261579-f0prnpsu 101 36 SV5 SV5 NNP cord-261579-f0prnpsu 101 37 fusion fusion NN cord-261579-f0prnpsu 101 38 protein protein NN cord-261579-f0prnpsu 101 39 Folding Folding NNP cord-261579-f0prnpsu 101 40 and and CC cord-261579-f0prnpsu 101 41 assembly assembly NN cord-261579-f0prnpsu 101 42 of of IN cord-261579-f0prnpsu 101 43 viral viral JJ cord-261579-f0prnpsu 101 44 membrane membrane NN cord-261579-f0prnpsu 101 45 proteins protein NNS cord-261579-f0prnpsu 101 46 Influence influence NN cord-261579-f0prnpsu 101 47 of of IN cord-261579-f0prnpsu 101 48 N n NN cord-261579-f0prnpsu 101 49 - - HYPH cord-261579-f0prnpsu 101 50 linked link VBN cord-261579-f0prnpsu 101 51 oligosaccharide oligosaccharide NN cord-261579-f0prnpsu 101 52 chains chain NNS cord-261579-f0prnpsu 101 53 on on IN cord-261579-f0prnpsu 101 54 the the DT cord-261579-f0prnpsu 101 55 processing processing NN cord-261579-f0prnpsu 101 56 , , , cord-261579-f0prnpsu 101 57 cell cell NN cord-261579-f0prnpsu 101 58 surface surface NN cord-261579-f0prnpsu 101 59 expression expression NN cord-261579-f0prnpsu 101 60 and and CC cord-261579-f0prnpsu 101 61 function function NN cord-261579-f0prnpsu 101 62 of of IN cord-261579-f0prnpsu 101 63 the the DT cord-261579-f0prnpsu 101 64 measles measles NNP cord-261579-f0prnpsu 101 65 virus virus NN cord-261579-f0prnpsu 101 66 fusion fusion NN cord-261579-f0prnpsu 101 67 protein protein NN cord-261579-f0prnpsu 101 68 Importance importance NN cord-261579-f0prnpsu 101 69 of of IN cord-261579-f0prnpsu 101 70 hemagglutinin hemagglutinin NN cord-261579-f0prnpsu 101 71 glycosylation glycosylation NN cord-261579-f0prnpsu 101 72 for for IN cord-261579-f0prnpsu 101 73 the the DT cord-261579-f0prnpsu 101 74 biological biological JJ cord-261579-f0prnpsu 101 75 functions function NNS cord-261579-f0prnpsu 101 76 of of IN cord-261579-f0prnpsu 101 77 influenza influenza NN cord-261579-f0prnpsu 101 78 virus virus NN cord-261579-f0prnpsu 101 79 Structure structure NN cord-261579-f0prnpsu 101 80 - - HYPH cord-261579-f0prnpsu 101 81 based base VBN cord-261579-f0prnpsu 101 82 , , , cord-261579-f0prnpsu 101 83 targeted target VBN cord-261579-f0prnpsu 101 84 deglycosylation deglycosylation NN cord-261579-f0prnpsu 101 85 of of IN cord-261579-f0prnpsu 101 86 HIV-1 HIV-1 NNP cord-261579-f0prnpsu 101 87 gp120 gp120 NNP cord-261579-f0prnpsu 101 88 and and CC cord-261579-f0prnpsu 101 89 effects effect NNS cord-261579-f0prnpsu 101 90 on on IN cord-261579-f0prnpsu 101 91 neutralization neutralization NN cord-261579-f0prnpsu 101 92 sensitivity sensitivity NN cord-261579-f0prnpsu 101 93 and and CC cord-261579-f0prnpsu 101 94 antibody antibody NN cord-261579-f0prnpsu 101 95 recognition recognition NN cord-261579-f0prnpsu 101 96 Influence influence NN cord-261579-f0prnpsu 101 97 of of IN cord-261579-f0prnpsu 101 98 N n NN cord-261579-f0prnpsu 101 99 - - HYPH cord-261579-f0prnpsu 101 100 glycans glycan NNS cord-261579-f0prnpsu 101 101 on on IN cord-261579-f0prnpsu 101 102 processing processing NN cord-261579-f0prnpsu 101 103 and and CC cord-261579-f0prnpsu 101 104 biological biological JJ cord-261579-f0prnpsu 101 105 activity activity NN cord-261579-f0prnpsu 101 106 of of IN cord-261579-f0prnpsu 101 107 the the DT cord-261579-f0prnpsu 101 108 nipah nipah NNP cord-261579-f0prnpsu 101 109 virus virus NN cord-261579-f0prnpsu 101 110 fusion fusion NN cord-261579-f0prnpsu 101 111 protein protein NN cord-261579-f0prnpsu 101 112 Carbohydrate Carbohydrate NNP cord-261579-f0prnpsu 101 113 masking masking NN cord-261579-f0prnpsu 101 114 of of IN cord-261579-f0prnpsu 101 115 an an DT cord-261579-f0prnpsu 101 116 antigenic antigenic JJ cord-261579-f0prnpsu 101 117 epitope epitope NN cord-261579-f0prnpsu 101 118 of of IN cord-261579-f0prnpsu 101 119 influenza influenza NN cord-261579-f0prnpsu 101 120 virus virus NN cord-261579-f0prnpsu 101 121 haemagglutinin haemagglutinin NNP cord-261579-f0prnpsu 101 122 independent independent JJ cord-261579-f0prnpsu 101 123 of of IN cord-261579-f0prnpsu 101 124 oligosaccharide oligosaccharide NN cord-261579-f0prnpsu 101 125 size size NN cord-261579-f0prnpsu 101 126 Role role NN cord-261579-f0prnpsu 101 127 of of IN cord-261579-f0prnpsu 101 128 conserved conserved JJ cord-261579-f0prnpsu 101 129 glycosylation glycosylation NN cord-261579-f0prnpsu 101 130 sites site NNS cord-261579-f0prnpsu 101 131 in in IN cord-261579-f0prnpsu 101 132 maturation maturation NN cord-261579-f0prnpsu 101 133 and and CC cord-261579-f0prnpsu 101 134 transport transport NN cord-261579-f0prnpsu 101 135 of of IN cord-261579-f0prnpsu 101 136 influenza influenza NN cord-261579-f0prnpsu 101 137 A a DT cord-261579-f0prnpsu 101 138 virus virus NN cord-261579-f0prnpsu 101 139 hemagglutinin hemagglutinin NN cord-261579-f0prnpsu 101 140 Functional functional JJ cord-261579-f0prnpsu 101 141 analysis analysis NN cord-261579-f0prnpsu 101 142 of of IN cord-261579-f0prnpsu 101 143 the the DT cord-261579-f0prnpsu 101 144 individual individual JJ cord-261579-f0prnpsu 101 145 oligosaccharide oligosaccharide NN cord-261579-f0prnpsu 101 146 chains chain NNS cord-261579-f0prnpsu 101 147 of of IN cord-261579-f0prnpsu 101 148 sendai sendai NNP cord-261579-f0prnpsu 101 149 virus virus NNP cord-261579-f0prnpsu 101 150 fusion fusion NNP cord-261579-f0prnpsu 101 151 protein protein NNP cord-261579-f0prnpsu 101 152 N N NNP cord-261579-f0prnpsu 101 153 - - HYPH cord-261579-f0prnpsu 101 154 Glycans Glycans NNP cord-261579-f0prnpsu 101 155 attached attach VBN cord-261579-f0prnpsu 101 156 to to IN cord-261579-f0prnpsu 101 157 the the DT cord-261579-f0prnpsu 101 158 stem stem NN cord-261579-f0prnpsu 101 159 domain domain NN cord-261579-f0prnpsu 101 160 of of IN cord-261579-f0prnpsu 101 161 haemagglutinin haemagglutinin NNP cord-261579-f0prnpsu 102 1 efficiently efficiently RB cord-261579-f0prnpsu 102 2 regulate regulate VB cord-261579-f0prnpsu 102 3 influenza influenza NN cord-261579-f0prnpsu 102 4 A a DT cord-261579-f0prnpsu 102 5 virus virus NN cord-261579-f0prnpsu 102 6 replication replication NN cord-261579-f0prnpsu 102 7 Post post JJ cord-261579-f0prnpsu 102 8 - - JJ cord-261579-f0prnpsu 102 9 translational translational JJ cord-261579-f0prnpsu 102 10 processing processing NN cord-261579-f0prnpsu 102 11 of of IN cord-261579-f0prnpsu 102 12 the the DT cord-261579-f0prnpsu 102 13 glycoproteins glycoprotein NNS cord-261579-f0prnpsu 102 14 of of IN cord-261579-f0prnpsu 102 15 lymphocytic lymphocytic JJ cord-261579-f0prnpsu 102 16 choriomeningitis choriomeningitis NNP cord-261579-f0prnpsu 102 17 virus virus NN cord-261579-f0prnpsu 102 18 Efficient efficient JJ cord-261579-f0prnpsu 102 19 selection selection NN cord-261579-f0prnpsu 102 20 for for IN cord-261579-f0prnpsu 102 21 highexpression highexpression NN cord-261579-f0prnpsu 102 22 transfectants transfectants NNP cord-261579-f0prnpsu 102 23 with with IN cord-261579-f0prnpsu 102 24 a a DT cord-261579-f0prnpsu 102 25 novel novel JJ cord-261579-f0prnpsu 102 26 eukaryotic eukaryotic JJ cord-261579-f0prnpsu 102 27 vector vector NN cord-261579-f0prnpsu 102 28 Recombinant recombinant JJ cord-261579-f0prnpsu 102 29 expression expression NN cord-261579-f0prnpsu 102 30 of of IN cord-261579-f0prnpsu 102 31 lymphocytic lymphocytic JJ cord-261579-f0prnpsu 102 32 choriomeningitis choriomeningitis NNP cord-261579-f0prnpsu 102 33 virus virus NN cord-261579-f0prnpsu 102 34 strain strain VBP cord-261579-f0prnpsu 102 35 WE we PRP cord-261579-f0prnpsu 102 36 glycoproteins glycoprotein NNS cord-261579-f0prnpsu 103 1 : : : cord-261579-f0prnpsu 103 2 a a DT cord-261579-f0prnpsu 103 3 single single JJ cord-261579-f0prnpsu 103 4 amino amino NN cord-261579-f0prnpsu 103 5 acid acid NN cord-261579-f0prnpsu 103 6 makes make VBZ cord-261579-f0prnpsu 103 7 the the DT cord-261579-f0prnpsu 103 8 difference difference NN cord-261579-f0prnpsu 103 9 Characterization characterization NN cord-261579-f0prnpsu 103 10 of of IN cord-261579-f0prnpsu 103 11 severe severe JJ cord-261579-f0prnpsu 103 12 acute acute JJ cord-261579-f0prnpsu 103 13 respiratory respiratory JJ cord-261579-f0prnpsu 103 14 syndrome syndrome NN cord-261579-f0prnpsu 103 15 coronavirus coronavirus NN cord-261579-f0prnpsu 103 16 membrane membrane NN cord-261579-f0prnpsu 103 17 protein protein NN cord-261579-f0prnpsu 104 1 We -PRON- PRP cord-261579-f0prnpsu 104 2 thank thank VBP cord-261579-f0prnpsu 104 3 E. E. NNP cord-261579-f0prnpsu 104 4 Lecompte Lecompte NNP cord-261579-f0prnpsu 104 5 , , , cord-261579-f0prnpsu 104 6 M. M. NNP cord-261579-f0prnpsu 104 7 Eickmann Eickmann NNP cord-261579-f0prnpsu 104 8 and and CC cord-261579-f0prnpsu 104 9 H.-D. H.-D. NNP cord-261579-f0prnpsu 105 1 Klenk Klenk NNP cord-261579-f0prnpsu 105 2 for for IN cord-261579-f0prnpsu 105 3 helpful helpful JJ cord-261579-f0prnpsu 105 4 discussions discussion NNS cord-261579-f0prnpsu 105 5 and and CC cord-261579-f0prnpsu 105 6 P. P. NNP cord-261579-f0prnpsu 105 7 Neubauer Neubauer NNP cord-261579-f0prnpsu 105 8 - - HYPH cord-261579-f0prnpsu 105 9 Rädel Rädel NNP cord-261579-f0prnpsu 105 10 for for IN cord-261579-f0prnpsu 105 11 excellent excellent JJ cord-261579-f0prnpsu 105 12 technical technical JJ cord-261579-f0prnpsu 105 13 assistance assistance NN cord-261579-f0prnpsu 105 14 . . . cord-261579-f0prnpsu 106 1 This this DT cord-261579-f0prnpsu 106 2 work work NN cord-261579-f0prnpsu 106 3 was be VBD cord-261579-f0prnpsu 106 4 supported support VBN cord-261579-f0prnpsu 106 5 by by IN cord-261579-f0prnpsu 106 6 the the DT cord-261579-f0prnpsu 106 7 Deutsche Deutsche NNP cord-261579-f0prnpsu 106 8 Forschungsgemeinschaft Forschungsgemeinschaft NNP cord-261579-f0prnpsu 106 9 , , , cord-261579-f0prnpsu 106 10 Sachbeihilfe Sachbeihilfe NNP cord-261579-f0prnpsu 106 11 Ga Ga NNP cord-261579-f0prnpsu 106 12 282/4 282/4 NNP cord-261579-f0prnpsu 106 13 - - SYM cord-261579-f0prnpsu 106 14 1 1 CD cord-261579-f0prnpsu 106 15 , , , cord-261579-f0prnpsu 106 16 SFB SFB NNP cord-261579-f0prnpsu 106 17 286 286 CD cord-261579-f0prnpsu 106 18 TP1 tp1 NN cord-261579-f0prnpsu 106 19 , , , cord-261579-f0prnpsu 106 20 and and CC cord-261579-f0prnpsu 106 21 the the DT cord-261579-f0prnpsu 106 22 Graduiertenkolleg Graduiertenkolleg NNP cord-261579-f0prnpsu 106 23 Protein Protein NNP cord-261579-f0prnpsu 106 24 Function Function NNP cord-261579-f0prnpsu 106 25 at at IN cord-261579-f0prnpsu 106 26 the the DT cord-261579-f0prnpsu 106 27 Atomic Atomic NNP cord-261579-f0prnpsu 106 28 Level Level NNP cord-261579-f0prnpsu 106 29 . . . cord-261579-f0prnpsu 107 1 The the DT cord-261579-f0prnpsu 107 2 author(s author(s NNP cord-261579-f0prnpsu 107 3 ) ) -RRB- cord-261579-f0prnpsu 107 4 declare declare VBP cord-261579-f0prnpsu 107 5 that that IN cord-261579-f0prnpsu 107 6 they -PRON- PRP cord-261579-f0prnpsu 107 7 have have VBP cord-261579-f0prnpsu 107 8 no no DT cord-261579-f0prnpsu 107 9 competing compete VBG cord-261579-f0prnpsu 107 10 interests interest NNS cord-261579-f0prnpsu 107 11 . . . cord-261579-f0prnpsu 108 1 RE RE NNP cord-261579-f0prnpsu 108 2 and and CC cord-261579-f0prnpsu 108 3 TS TS NNP cord-261579-f0prnpsu 108 4 carried carry VBD cord-261579-f0prnpsu 108 5 out out RP cord-261579-f0prnpsu 108 6 experiments experiment NNS cord-261579-f0prnpsu 108 7 , , , cord-261579-f0prnpsu 108 8 participated participate VBD cord-261579-f0prnpsu 108 9 in in IN cord-261579-f0prnpsu 108 10 the the DT cord-261579-f0prnpsu 108 11 analysis analysis NN cord-261579-f0prnpsu 108 12 of of IN cord-261579-f0prnpsu 108 13 the the DT cord-261579-f0prnpsu 108 14 results result NNS cord-261579-f0prnpsu 108 15 and and CC cord-261579-f0prnpsu 108 16 drafted draft VBD cord-261579-f0prnpsu 108 17 the the DT cord-261579-f0prnpsu 108 18 manuscript manuscript NN cord-261579-f0prnpsu 108 19 . . . cord-261579-f0prnpsu 109 1 OL OL NNP cord-261579-f0prnpsu 109 2 helped help VBD cord-261579-f0prnpsu 109 3 to to TO cord-261579-f0prnpsu 109 4 draft draft VB cord-261579-f0prnpsu 109 5 the the DT cord-261579-f0prnpsu 109 6 manuscript manuscript NN cord-261579-f0prnpsu 109 7 and and CC cord-261579-f0prnpsu 109 8 revised revise VBD cord-261579-f0prnpsu 109 9 it -PRON- PRP cord-261579-f0prnpsu 109 10 critically critically RB cord-261579-f0prnpsu 109 11 . . . cord-261579-f0prnpsu 110 1 WG WG NNP cord-261579-f0prnpsu 110 2 designed design VBD cord-261579-f0prnpsu 110 3 the the DT cord-261579-f0prnpsu 110 4 study study NN cord-261579-f0prnpsu 110 5 , , , cord-261579-f0prnpsu 110 6 participated participate VBD cord-261579-f0prnpsu 110 7 in in IN cord-261579-f0prnpsu 110 8 the the DT cord-261579-f0prnpsu 110 9 analysis analysis NN cord-261579-f0prnpsu 110 10 of of IN cord-261579-f0prnpsu 110 11 the the DT cord-261579-f0prnpsu 110 12 results result NNS cord-261579-f0prnpsu 110 13 and and CC cord-261579-f0prnpsu 110 14 helped help VBD cord-261579-f0prnpsu 110 15 to to TO cord-261579-f0prnpsu 110 16 draft draft VB cord-261579-f0prnpsu 110 17 the the DT cord-261579-f0prnpsu 110 18 manuscript manuscript NN cord-261579-f0prnpsu 110 19 . . . cord-261579-f0prnpsu 111 1 All all DT cord-261579-f0prnpsu 111 2 authors author NNS cord-261579-f0prnpsu 111 3 read read VBP cord-261579-f0prnpsu 111 4 and and CC cord-261579-f0prnpsu 111 5 approved approve VBD cord-261579-f0prnpsu 111 6 the the DT cord-261579-f0prnpsu 111 7 final final JJ cord-261579-f0prnpsu 111 8 manuscript manuscript NN cord-261579-f0prnpsu 111 9 . . .