id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-342117-r2chpw7y	Wu, Xinwei	Inhibitory effect of small interfering RNA on dengue virus replication in mosquito cells	2010-10-14		.txt	text/plain	2928	191	59	The antiviral effect of siRNA was evaluated by measurement of cell survival rate using the MTT method and viral RNA was quantitated with real-time RT-PCR. CONCLUSIONS: Our data showed that synthetic siRNA against the DEN-1 membrane glycoprotein precursor gene effectively inhibited DEN-1 viral RNA replication and increased C6/36 cell survival rate. Therefore, in this study, we designed and synthesized 21 nt siRNAs against the DEN-I viral PrM gene, and investigated their inhibition effects of dengue virus replication in transfected C6/36 cells. These data indicate that siRNA against DEN viral genome can effectively inhibit viral RNA replication in the C6/36 cells, protect host cell from viral attack, suggesting its potential role in prevention and treatment of dengue fever. Our data showed that synthetic siRNA against the DEN membrane glycoprotein precursor gene effectively inhibited DEN viral RNA replication and increased C6/36 cell survival rate. Inhibition of viral gene expression and replication in mosquito cells by dsRNA-triggered RNA interference	./cache/cord-342117-r2chpw7y.txt	./txt/cord-342117-r2chpw7y.txt