id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-341168-3gd1w2kn	Beer, Christiane	Amphotropic murine leukemia virus is preferentially attached to cholesterol-rich microdomains after binding to mouse fibroblasts	2006-04-02		.txt	text/plain	3191	158	57	We have previously shown that A-MLV entry is closely associated with cholesterol-rich microdomains like rafts and caveolae [1] and that A-MLV envelope protein is associated with rafts in infected cells suggesting a possible role of rafts in A-MLV assembly [2] . As rafts are suggested to be pre-caveolae [11] and a large fraction of the A-MLV receptor Pit2 was found associated with cholesterol-rich microdomains [1] , we have here investigated if rafts and caveolae are involved in the early steps of A-MLV binding. As GM1 is a general marker for cholesterol-rich microdomains, we investigated if these regions of preferred A-MLV binding were also enriched in caveolin-1 (cav-1), a major structural protein of caveolae. Using confocal microscopy we found that A-MLV binds preferentially to large GM1-positive membrane regions of NIH3T3 cells, which are most likely rafts. In addition, A-MLV binding to large rafts was independent of plasma membrane cholesterol indicating that the A-MLV receptor Pit2 or other virus interacting proteins were still present in these regions.	./cache/cord-341168-3gd1w2kn.txt	./txt/cord-341168-3gd1w2kn.txt