id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-335865-pibq3iwh	Jumat, Muhammad Raihan	Imaging analysis of human metapneumovirus-infected cells provides evidence for the involvement of F-actin and the raft-lipid microdomains in virus morphogenesis	2014-11-19		.txt	text/plain	5427	265	46	title: Imaging analysis of human metapneumovirus-infected cells provides evidence for the involvement of F-actin and the raft-lipid microdomains in virus morphogenesis METHODS: HMPV-infected LLC-MK2 cells were stained with antibodies that recognised the HMPV fusion protein (F protein), attachment protein (G protein) and matrix protein (M protein), and fluorescent probes that detect GM1 within lipid-raft membranes (CTX-B-AF488) and F-actin (Phalloidin-FITC). Cells co-expressing recombinant HMPV G and F proteins formed virus-like particles and were co-stained with antibodies that recognise the recombinant G and F proteins and phalloidin-FITC and CTX-B-AF594, and the distribution of the G and F proteins, GM1 and F-actin determined. We also observed these co-stained filamentous structures spreading to the non-infected cells, suggesting that F-actin may play a role in virus transmission in the monolayer, in a similar manner to that proposed for RSV [17, 25, 30] . HMPV-infected cells were stained with anti-G and CTX-B-AF488, and examined using confocal microscopy at an optical plane that allowed the virus filaments to be visualized ( Figure 4D ).	./cache/cord-335865-pibq3iwh.txt	./txt/cord-335865-pibq3iwh.txt