id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-333913-roftm446	Holtz, Lori R	Klassevirus 1, a previously undescribed member of the family Picornaviridae, is globally widespread	2009-06-24		.txt	text/plain	3138	170	51	In an effort to identify novel viruses that may be causal agents of diarrhea, we used high throughput mass sequencing to analyze stool samples collected from patients with acute diarrhea. RESULTS: Sequences with limited similarity to known picornaviruses were detected in a stool sample collected in Australia from a child with acute diarrhea. We also detected klassevirus 1 by RT-PCR in a diarrhea specimen collected from a patient in St. Louis, United States as well as in untreated sewage collected in Barcelona, Spain. Extracted nucleic acid from a stool specimen collected in 1984 from a child with acute diarrhea was subjected to high throughput mass sequencing using 454 pyroseqencing technology. Phylogenetic analysis of the VP3/VP1, P2, and P3 regions of the genome demonstrated that this virus sequence is highly divergent from all previously described picornaviruses (Figure 2A -C).	./cache/cord-333913-roftm446.txt	./txt/cord-333913-roftm446.txt