id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-322238-8iwljdoi	Chen, Qin	Detection of swine transmissible gastroenteritis coronavirus using loop-mediated isothermal amplification	2010-08-29		.txt	text/plain	1708	87	50	Loop-mediated isothermal amplification (LAMP) was developed to detect the TGEV by incubation at 60°C for 1 h and the product specificity was confirmed by HphI digestion. By using serial sample dilutions as templates, the detection limit of LAMP was about 10 pg RNA, 10 times more sensitive than that of PCR and could be comparable to the nest-PCR. Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method, which amplifies DNA/RNA with high specificity, sensitivity and rapidity under isothermal condition [5] . As a kind of nucleic acid amplification method, LAMP could not only qualitatively detect the TGEV, but also quantitatively analyze the virus. In conclusion, this study demonstrates that the LAMP method established could detect only the TGEV and no cross-reaction with other viruses, the detection limit was about 10 pg RNA, which was 10 times more sensitive than that of PCR and could be comparable to the nest-PCR.	./cache/cord-322238-8iwljdoi.txt	./txt/cord-322238-8iwljdoi.txt