id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-319954-lh32rhhe	Loo, Liat Hui	Evidence for the interaction of the human metapneumovirus G and F proteins during virus-like particle formation	2013-09-25		.txt	text/plain	5843	285	48	Immunoblotting of lysates prepared from pCAGGS/M transfected cells with MAbM3F8 (anti-M) revealed the presence of a 28 kDa protein, the expected size of the HMPV M protein ( Figure 1C ). Only pCAGGS/F-cmyc transfected cells immunoprecipitated and immunoblotted with anti-cmyc showed the presence of F proteins species corresponding in size to the monomeric and oligomeric F protein ( Figure 1D ). Similarly lysates immunoprecipitated with anti-FLAG and immunoblotted using anti-cmyc revealed increasing amounts of F145 with increasing DSP concentration, together with a cmyc-tagged protein species >300 kDa ( Figure 2F ) whose molecular mass was consistent with higher oligomeric forms of the F protein. We failed to detect the presence of the M protein in detergent extracts prepared from pCAGGS/M transfected cells that were immunoprecipitated with influenza virus anti-NP (i.e. a non-specific antibody) and immunblotted with anti-M ( Figure 4C ); confirming the specificity of the M protein immunoprecipitation.	./cache/cord-319954-lh32rhhe.txt	./txt/cord-319954-lh32rhhe.txt