id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-315688-ba5dus2j	He, Lei	In vitro inhibition of transmissible gastroenteritis coronavirus replication in swine testicular cells by short hairpin RNAs targeting the ORF 7 gene	2012-08-28		.txt	text/plain	4249	212	51	title: In vitro inhibition of transmissible gastroenteritis coronavirus replication in swine testicular cells by short hairpin RNAs targeting the ORF 7 gene In the reporter assays, three of four shRNA expression plasmids were able to inhibit significantly the expression of ORF 7 gene and replication of TGEV, as shown by real-time quantitative RT-PCR analysis of viral ORF 7 and N genes and detection of virus titers (TCID(50)/ml). Here, we demonstrate that RNAi targeting of the ORF 7 gene of TGEV, introduced by short hairpin RNAs (shRNAs), is capable of inhibiting virus replication and protecting swine testicular (ST) cells from the destruction induced by TGEV, which may be not only a new anti-TGEV strategy, but also a new approach to the study of its pathogenesis. The present study demonstrated the use of RNAi against TGEV via shRNA-expressing plasmid vector pGPU6-GFP, which significantly reduced viral genomic RNA replication and protected ST cells from TGEV destruction, by targeting ORF 7 gene.	./cache/cord-315688-ba5dus2j.txt	./txt/cord-315688-ba5dus2j.txt